FastQCFastQC Report
Fri 27 May 2016
SRR939135_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939135_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233423
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53470.4335090232629033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43070.3491908290991817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23700.19214819246925022No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16200.1313418024473356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83750.029.8720971
GGTATCA57550.028.5410981
ATCAACG101250.024.7982373
TATCAAC101200.024.7168662
TCAACGC103600.024.235734
CAACGCA103700.024.2123585
AACGCAG105700.023.7893896
GAGTACT50150.022.1901712-13
GAAACGA1303.0032144E-421.8560496
GTACTTT50500.021.70949714-15
ACGCAGA116350.021.4083467
GTATAGG3351.8189894E-1221.2562371
CGCAGAG116500.021.137758
AGTACTT52300.020.9610512-13
GTACATG67750.020.250161
TACATGG66950.019.9543912
AGAGTAC96350.019.7823510-11
GTAAGGT5750.019.773494
GCAGAGT122150.019.6949
GTATATG4950.019.1807141
Produced by FastQC (version 0.11.5)