FastQCFastQC Report
Fri 27 May 2016
SRR939135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233423
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54480.4416976171191878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44400.35997382892973456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24180.19603980143065272No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18760.15209705024148243No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13310.1079110734922245No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC12450.10093860743637828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAC650.00587620729.1981533
GGTATCA68650.028.9250411
GTATCAA96450.028.0430071
GTAAGGT7300.027.9482334
TAAGGTG7650.027.9099985
GGTAAGG7250.024.868773
GAGTACT54500.023.46422412-13
ATCAACG115250.023.2191813
TCAACGC115400.023.1890014
TATCAAC116050.023.181772
CAACGCA116450.022.9799125
AGGTAAG8150.022.7046992
GTACTTT56050.022.60370314-15
AACGCAG120050.022.4093676
AGTACTT56550.020.34806312-13
ACGCAGA132600.020.1452927
CGCAGAG132400.020.1398878
AGAGTAC108050.019.27896310-11
GCAGAGT139850.018.8973739
ACTTTTT67450.018.88811716-17
Produced by FastQC (version 0.11.5)