FastQCFastQC Report
Fri 27 May 2016
SRR939134_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939134_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214021
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53080.43722472675513846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42090.34669910981770496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21970.18096886297683484No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16250.13385270930239263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62600.029.049991
GTATCAA90250.028.9885141
GTGTAGC7850.024.7990911
ATCAACG106900.024.3675843
TATCAAC106750.024.3110872
TCAACGC107250.024.2439024
CAACGCA108050.024.0205695
GAGTACT48050.023.88982812-13
GTACTTT48500.023.47345214-15
AACGCAG111000.023.3260466
ACGCAGA120450.021.4566867
CGCAGAG120250.021.4530098
GTATATG5050.020.6848751
TAGACCG1150.003786812320.5920544
AGTACTT49750.020.3141912-13
ACTTTTT57000.020.18189416-17
GCAGAGT125750.020.1006499
GTATAGC6200.019.145641
AGAGTAC97900.018.95348510-11
TACATGG69800.018.861712
Produced by FastQC (version 0.11.5)