##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939134_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1214021 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98229684659491 33.0 31.0 34.0 30.0 34.0 2 32.10507725978381 34.0 31.0 34.0 30.0 34.0 3 32.14347445390154 34.0 31.0 34.0 30.0 34.0 4 35.59953987616359 37.0 35.0 37.0 33.0 37.0 5 35.648449244288194 37.0 35.0 37.0 33.0 37.0 6 35.73127235855063 37.0 36.0 37.0 35.0 37.0 7 35.66768532010567 37.0 36.0 37.0 33.0 37.0 8 35.659821370470524 37.0 36.0 37.0 33.0 37.0 9 37.30134075110727 39.0 37.0 39.0 34.0 39.0 10-11 37.20509735828293 39.0 37.0 39.0 34.0 39.0 12-13 37.104632045079946 39.0 37.0 39.0 33.5 39.0 14-15 38.34324941660812 40.0 38.0 41.0 33.5 41.0 16-17 38.25502318328925 40.0 38.0 41.0 33.0 41.0 18-19 38.18276248928149 40.0 38.0 41.0 33.0 41.0 20-21 38.180463929371896 40.0 38.0 41.0 33.5 41.0 22-23 38.21990393905871 40.0 38.0 41.0 33.5 41.0 24-25 38.16422080013443 40.0 38.0 41.0 33.0 41.0 26-27 38.03996471230728 40.0 38.0 41.0 33.0 41.0 28-29 37.898075898192864 40.0 38.0 41.0 33.0 41.0 30-31 37.66723886983833 40.0 37.5 41.0 32.5 41.0 32-33 37.447169365274576 40.0 37.0 41.0 32.0 41.0 34-35 37.127880819195056 40.0 37.0 41.0 31.0 41.0 36-37 36.85204250997306 40.0 36.0 41.0 30.0 41.0 38-39 36.65373786779635 40.0 36.0 41.0 30.0 41.0 40-41 36.460342942996874 40.0 36.0 41.0 30.0 41.0 42-43 36.231925971626524 39.0 35.5 41.0 30.0 41.0 44-45 35.92696378398726 39.0 35.0 41.0 28.5 41.0 46-47 35.72696312502008 39.0 35.0 41.0 27.0 41.0 48-49 35.55748459046425 39.0 35.0 41.0 26.0 41.0 50-51 34.42644031693027 38.0 34.0 40.0 24.5 40.5 52-53 34.570248372968834 38.5 34.5 39.5 24.0 40.5 54-55 35.092533407576965 39.0 35.0 40.5 25.0 41.0 56-57 35.000774698296 39.0 35.0 41.0 25.0 41.0 58-59 34.79335077399814 39.0 35.0 41.0 24.5 41.0 60-61 34.49564134393062 38.0 34.5 40.0 23.5 41.0 62-63 34.11589914836729 38.0 34.0 40.0 22.5 41.0 64-65 33.74708592355486 37.0 34.0 40.0 22.0 41.0 66-67 33.34982796837946 36.5 33.5 39.5 20.5 41.0 68-69 32.9468584151345 36.0 33.0 39.0 19.5 41.0 70-71 32.515062342414176 35.5 33.0 39.0 18.0 40.5 72-73 32.07802542130655 35.0 32.0 38.0 14.5 40.0 74-75 31.63942180571835 35.0 32.0 37.0 12.0 39.5 76-77 31.19681414077681 35.0 31.0 37.0 7.5 39.0 78-79 30.779719625937275 35.0 31.0 36.0 6.5 39.0 80-81 30.334852527262708 35.0 31.0 36.0 2.0 37.5 82-83 29.939748159216357 34.0 30.0 35.0 2.0 37.0 84-85 29.55916454492962 34.0 30.0 35.0 2.0 37.0 86-87 29.158281858386303 34.0 29.5 35.0 2.0 36.0 88-89 28.732633537640616 34.0 29.0 35.0 2.0 36.0 90-91 28.308037916971784 34.0 29.0 35.0 2.0 35.5 92-93 27.890624626756868 34.0 28.0 35.0 2.0 35.0 94-95 27.40723471834507 34.0 27.0 35.0 2.0 35.0 96-97 26.799661620350882 33.5 25.5 35.0 2.0 35.0 98-99 26.0589602651025 33.0 24.5 35.0 2.0 35.0 100-101 23.12521035468085 30.0 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3489398969480142 1101 2 0.4823441136981117 1101 3 0.43244130053449936 1101 4 0.3261001529826828 1101 5 0.39815601121849653 1101 6 0.364502057881694 1101 7 0.36744153226771914 1101 8 0.3694340519121937 1101 9 0.5647639089357952 1101 10-11 0.45423656403154666 1101 12-13 0.46812416201647267 1101 14-15 0.6084524757884751 1101 16-17 0.7060240578913053 1101 18-19 0.7680780721495708 1101 20-21 0.7000650283743042 1101 22-23 0.7966083117565432 1101 24-25 0.7941268533462136 1101 26-27 0.7037014146725653 1101 28-29 0.655587547208988 1101 30-31 0.6056098869470361 1101 32-33 0.6046007135459135 1101 34-35 0.6753806813518679 1101 36-37 0.6159128217955967 1101 38-39 0.5959762009082468 1101 40-41 0.6422484315821464 1101 42-43 0.623560684745101 1101 44-45 0.6338947216473301 1101 46-47 0.6938732663096019 1101 48-49 0.6679976459573638 1101 50-51 0.43266248621903713 1101 52-53 0.4347104524748815 1101 54-55 0.5294655638898718 1101 56-57 0.5572638166930659 1101 58-59 0.603819235187423 1101 60-61 0.4887784043202785 1101 62-63 0.3470122815734271 1101 64-65 0.47052043058652515 1101 66-67 0.3846230996171798 1101 68-69 0.5364283082240178 1101 70-71 0.4147685821312379 1101 72-73 0.32218414799631034 1101 74-75 0.32653741871294883 1101 76-77 0.2975187690134291 1101 78-79 0.20187132800683472 1101 80-81 0.25200845386975956 1101 82-83 0.3224312873363111 1101 84-85 0.23745648820509757 1101 86-87 -0.1462630027077232 1101 88-89 0.013807033352534859 1101 90-91 0.12585160293019015 1101 92-93 -0.052964136032187525 1101 94-95 -0.2845546462861286 1101 96-97 -0.37133789077664403 1101 98-99 -0.536228114868333 1101 100-101 -0.4895526604244509 1102 1 0.2944288704193134 1102 2 0.4051966661530244 1102 3 0.5388429989833199 1102 4 0.2777479161179315 1102 5 0.2602144240869251 1102 6 0.37404626919320805 1102 7 0.3220730568550607 1102 8 0.440250569709562 1102 9 0.3677400197175018 1102 10-11 0.4298500521634452 1102 12-13 0.3628461557811775 1102 14-15 0.40910469543921835 1102 16-17 0.4499943732901954 1102 18-19 0.5055740077309281 1102 20-21 0.48320677616995056 1102 22-23 0.6251950416687535 1102 24-25 0.6385122176137656 1102 26-27 0.6804529400249706 1102 28-29 0.6982591613091742 1102 30-31 0.7160377506885922 1102 32-33 0.7900912171512857 1102 34-35 0.6605192541193077 1102 36-37 0.583607866073848 1102 38-39 0.5356984815730925 1102 40-41 0.5097196937514994 1102 42-43 0.6852442147644169 1102 44-45 0.681194991333463 1102 46-47 0.7911228503043972 1102 48-49 1.0042111379146021 1102 50-51 0.9023897398196326 1102 52-53 0.9645385036578631 1102 54-55 0.9283762996930989 1102 56-57 0.9203407250571587 1102 58-59 0.9163873739705792 1102 60-61 0.77260393078204 1102 62-63 0.7665128840637294 1102 64-65 0.9020325855891116 1102 66-67 0.7764665286476529 1102 68-69 0.8118715689397433 1102 70-71 0.7406967040829286 1102 72-73 0.7926143069389866 1102 74-75 0.5889506314207189 1102 76-77 0.35161857200866464 1102 78-79 0.4219237350990035 1102 80-81 0.3604662699858956 1102 82-83 0.0845002198765279 1102 84-85 0.34146257048806206 1102 86-87 0.35881991143262937 1102 88-89 0.31667794741638033 1102 90-91 0.40661686123594976 1102 92-93 0.34530585612587217 1102 94-95 0.08350954283844558 1102 96-97 0.16738884640370344 1102 98-99 0.2886862932566814 1102 100-101 -0.08028482958076921 1103 1 0.20446626591143868 1103 2 0.186168577777174 1103 3 0.20508591193441106 1103 4 0.36640214236529545 1103 5 0.38155026901162614 1103 6 0.3262023095395108 1103 7 0.44685151408222623 1103 8 0.3967214745320007 1103 9 0.5023637913730141 1103 10-11 0.4335896013760987 1103 12-13 0.4762203798015179 1103 14-15 0.7012627772267805 1103 16-17 0.6610654804009073 1103 18-19 0.6018094044664721 1103 20-21 0.64800676132311 1103 22-23 0.6676199027115572 1103 24-25 0.802787923534872 1103 26-27 0.6181193870820465 1103 28-29 0.7194112765206953 1103 30-31 0.8099486881740958 1103 32-33 0.7407347351113316 1103 34-35 0.6965970887068877 1103 36-37 0.45606418387058056 1103 38-39 0.7241931042312331 1103 40-41 0.8022164692022358 1103 42-43 0.6582600730512738 1103 44-45 0.7388378690232926 1103 46-47 0.8934268951418787 1103 48-49 0.7618595831714927 1103 50-51 0.8092629013625867 1103 52-53 0.8978251312897001 1103 54-55 0.8175724358015515 1103 56-57 0.9864184668519584 1103 58-59 1.0142327255160453 1103 60-61 1.0198085481509978 1103 62-63 0.6910073953705265 1103 64-65 0.7520052117177016 1103 66-67 0.720995002113554 1103 68-69 0.4952997055678381 1103 70-71 0.7754383225746722 1103 72-73 0.8268223669202399 1103 74-75 0.9075592964109269 1103 76-77 0.8014130359911462 1103 78-79 0.8198841496971667 1103 80-81 0.8580629368690786 1103 82-83 0.6794225987123461 1103 84-85 0.7064722673340356 1103 86-87 0.6901272689725069 1103 88-89 0.6186767039930672 1103 90-91 0.7134037254893855 1103 92-93 0.5056657065439047 1103 94-95 0.6077284595754797 1103 96-97 0.44102548841842193 1103 98-99 0.2558051700928168 1103 100-101 0.09229583054709778 1104 1 0.16740562034510376 1104 2 0.3044527371539658 1104 3 0.22398059499702327 1104 4 0.34164726474230633 1104 5 0.34885554046403655 1104 6 0.28091673115961413 1104 7 0.29050652015222767 1104 8 0.2605844622542648 1104 9 0.46166835172228815 1104 10-11 0.33699608971176787 1104 12-13 0.368581546523167 1104 14-15 0.4330551735482615 1104 16-17 0.3953195107861518 1104 18-19 0.34404252457564155 1104 20-21 0.35772733302439974 1104 22-23 0.4847598907317092 1104 24-25 0.4241719480583157 1104 26-27 0.3586336075432399 1104 28-29 0.440899083664398 1104 30-31 0.4486606465585439 1104 32-33 0.38677113590443213 1104 34-35 0.5154751953787624 1104 36-37 0.5757633423985311 1104 38-39 0.280750628311047 1104 40-41 0.6216463214401315 1104 42-43 0.6951353353106029 1104 44-45 0.5938112667189301 1104 46-47 1.053287365174647 1104 48-49 1.0140401393054006 1104 50-51 1.1131337688763878 1104 52-53 1.089553512312854 1104 54-55 1.0170636927564303 1104 56-57 0.9893213372101712 1104 58-59 1.0309084144372562 1104 60-61 1.0217592770317125 1104 62-63 1.1121830538616422 1104 64-65 1.2172060022593882 1104 66-67 1.023901716272512 1104 68-69 0.9183996668095205 1104 70-71 0.985036773522971 1104 72-73 0.8860669479619787 1104 74-75 0.8229976818864806 1104 76-77 0.7410785772783726 1104 78-79 0.8644714376074525 1104 80-81 0.7625773206918076 1104 82-83 0.6942472573347942 1104 84-85 0.7085154876703044 1104 86-87 0.6751152250634895 1104 88-89 0.701771267897854 1104 90-91 0.6857011331660985 1104 92-93 0.6316130432704625 1104 94-95 0.5502215967895552 1104 96-97 0.5955995312319544 1104 98-99 0.44165533873957585 1104 100-101 -0.03461766151384893 1105 1 0.28761296796113456 1105 2 0.32609589215628887 1105 3 0.42682990929645825 1105 4 0.2712268930261672 1105 5 0.23191996855826602 1105 6 0.21395059357037383 1105 7 0.21109656260109944 1105 8 0.3175372195596182 1105 9 0.3290171841443694 1105 10-11 0.3987259046646301 1105 12-13 0.295738922365679 1105 14-15 0.32069509732119883 1105 16-17 0.3147461858947693 1105 18-19 0.49860039820213586 1105 20-21 0.48935899470595245 1105 22-23 0.5863120348520283 1105 24-25 0.6115972882574994 1105 26-27 0.5128230555207551 1105 28-29 0.44489289567297163 1105 30-31 0.6875100201179762 1105 32-33 0.64512679570214 1105 34-35 0.5839138659586709 1105 36-37 0.6906934311949655 1105 38-39 0.6023445039461421 1105 40-41 0.7854413473429886 1105 42-43 0.6084679772772859 1105 44-45 0.558038500742903 1105 46-47 0.5574527192843206 1105 48-49 0.531227098362983 1105 50-51 0.6803291490916621 1105 52-53 0.5742952322875325 1105 54-55 0.6265352339218566 1105 56-57 0.6624034185460772 1105 58-59 0.6159184418527914 1105 60-61 0.8008224076438708 1105 62-63 0.7592702689847926 1105 64-65 0.7726733609164924 1105 66-67 0.7266590216268085 1105 68-69 0.6943781585489859 1105 70-71 0.6289177108290076 1105 72-73 0.4274916363011698 1105 74-75 0.41245259906283493 1105 76-77 0.3919221745474175 1105 78-79 0.28934301495266723 1105 80-81 0.2800426457952767 1105 82-83 0.12881837089254589 1105 84-85 0.25726424399007186 1105 86-87 0.1828997276381834 1105 88-89 0.2253610358793665 1105 90-91 0.3427011425229374 1105 92-93 0.3071181567845471 1105 94-95 0.23483481613576274 1105 96-97 0.30560020180562475 1105 98-99 0.43141817398742077 1105 100-101 0.5656307626380297 1106 1 0.12406264496008745 1106 2 0.2031603116125993 1106 3 0.25729347237849964 1106 4 0.24385496563722597 1106 5 0.26588558295896547 1106 6 0.19103749323119246 1106 7 0.2503799605143868 1106 8 0.15463548083731382 1106 9 0.2514711111171195 1106 10-11 0.37869308052817985 1106 12-13 0.2301931774278998 1106 14-15 0.24702268250673853 1106 16-17 0.33147381239528073 1106 18-19 0.3032899511346656 1106 20-21 0.39558806191416807 1106 22-23 0.32160003209364874 1106 24-25 0.16872131063441032 1106 26-27 0.3665551492024548 1106 28-29 0.26293538629735025 1106 30-31 0.2711356214254792 1106 32-33 0.10776788556675143 1106 34-35 0.27272138224542886 1106 36-37 0.3808941885348247 1106 38-39 0.21296381839103873 1106 40-41 0.4199796753322076 1106 42-43 0.49286580398832314 1106 44-45 0.4118864130457993 1106 46-47 0.21954182899227703 1106 48-49 0.19669431447824337 1106 50-51 0.15496259264887868 1106 52-53 -0.047707723555582504 1106 54-55 -0.00413057505412695 1106 56-57 0.17451348952580759 1106 58-59 0.1635107815712118 1106 60-61 0.35848223354379627 1106 62-63 0.2982772211399265 1106 64-65 0.3516309021896902 1106 66-67 0.1641750653538523 1106 68-69 0.22642004463813947 1106 70-71 0.1839380113650364 1106 72-73 0.08068245830199317 1106 74-75 0.1801208246608006 1106 76-77 0.24071545843442976 1106 78-79 0.2603249442098914 1106 80-81 0.2555092239587964 1106 82-83 0.2014533546475583 1106 84-85 0.06544126648698168 1106 86-87 0.1293645635858489 1106 88-89 0.229703664574739 1106 90-91 0.2211079187856626 1106 92-93 0.06071081244097343 1106 94-95 0.4741848192495759 1106 96-97 0.5330713914489316 1106 98-99 0.4679109127294758 1106 100-101 0.557134992106235 1107 1 0.08581165235848331 1107 2 0.1164677145610824 1107 3 0.13052196699030105 1107 4 0.08304174525688524 1107 5 0.22046698511071838 1107 6 0.16359597256993652 1107 7 0.14569926145237844 1107 8 0.05378260049192818 1107 9 0.17396072205845314 1107 10-11 0.12541870795088528 1107 12-13 0.2177189571401712 1107 14-15 0.08926150076326422 1107 16-17 0.35927804974944166 1107 18-19 0.4134073581549771 1107 20-21 0.2820423022817806 1107 22-23 0.2232174471988273 1107 24-25 0.23083149125042013 1107 26-27 0.31241591597157736 1107 28-29 0.37996168270634456 1107 30-31 0.4902209138699405 1107 32-33 0.4973638331356369 1107 34-35 0.4468411970871884 1107 36-37 0.585988244978978 1107 38-39 0.5207158862804349 1107 40-41 0.5772645847008064 1107 42-43 0.6637373383350607 1107 44-45 0.8154886638737722 1107 46-47 0.9408240012647013 1107 48-49 0.601206113244352 1107 50-51 0.5987819296012873 1107 52-53 0.6534644356781527 1107 54-55 0.552681442885465 1107 56-57 0.6116466385240926 1107 58-59 0.6012723455085762 1107 60-61 0.5573630669616492 1107 62-63 0.5692482728933896 1107 64-65 0.7034782216141124 1107 66-67 0.6785709123763368 1107 68-69 0.7530067739846089 1107 70-71 0.8131188081804837 1107 72-73 0.7336000675910697 1107 74-75 0.8096136239810825 1107 76-77 0.8000113691068975 1107 78-79 0.6936295395033234 1107 80-81 0.7509541343857791 1107 82-83 0.6331042264171032 1107 84-85 0.7078533214699831 1107 86-87 0.7126104333194725 1107 88-89 0.6316657891611257 1107 90-91 0.6592597614746616 1107 92-93 0.5388037531729957 1107 94-95 0.6502766075200448 1107 96-97 0.6988321851186257 1107 98-99 0.5933076226281493 1107 100-101 0.5273286468058238 1108 1 -0.1475911430745498 1108 2 -0.09622755252757287 1108 3 0.0035656745135739243 1108 4 0.1766584028333611 1108 5 0.044696002884755615 1108 6 0.07243765040921346 1108 7 -0.13725847676314373 1108 8 0.034895283373181485 1108 9 0.09196502305895393 1108 10-11 0.14532405406987436 1108 12-13 0.11468435429461721 1108 14-15 0.07064326779505592 1108 16-17 0.0032511080157675565 1108 18-19 -0.045093202105029206 1108 20-21 0.18000146378224713 1108 22-23 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-2.4379845133344986 2316 92-93 -2.1554261026370156 2316 94-95 -1.8289931125817382 2316 96-97 -2.0423162457549964 2316 98-99 -2.5640469151157035 2316 100-101 -2.4331989189278502 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4189.0 3 1166.0 4 1230.0 5 1278.0 6 1508.0 7 1793.0 8 2303.0 9 2675.0 10 3308.0 11 4081.0 12 5223.0 13 7973.0 14 6941.0 15 7275.0 16 9070.0 17 12728.0 18 10811.0 19 7467.0 20 6935.0 21 7451.0 22 8024.0 23 8487.0 24 9643.0 25 10886.0 26 12695.0 27 14528.0 28 16985.0 29 20252.0 30 23821.0 31 29599.0 32 36794.0 33 47947.0 34 67657.0 35 101020.0 36 162290.0 37 255330.0 38 242464.0 39 40188.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.07761984980164 19.901488668964053 16.669602489577827 21.351288991656475 2 15.077258269484034 20.881215152962053 40.02932490860706 24.01220166894685 3 18.799841003590615 25.900251194534746 30.4415449041156 24.858362897759044 4 12.948625637447389 17.961850892364488 38.147705519306726 30.941817950881394 5 13.68077504239441 37.80839046406207 33.735062981267276 14.775771512276235 6 29.85030335003957 36.820265101556316 18.16901872856766 15.160412819836456 7 27.238821965962188 33.79139722540074 21.227135277377485 17.742645531259583 8 26.452565984882646 33.5058196617927 20.354018777570126 19.68759557575453 9 26.703445183000873 17.34215314251043 20.965650393602118 34.988751280886575 10-11 24.907167084409636 27.062448761053084 26.82120065140699 21.20918350313029 12-13 26.29675459951245 24.533371145638654 27.347241446683412 21.82263280816548 14-15 23.26468517691127 25.617099746598367 26.531703231571502 24.58651184491886 16-17 21.739291721778283 28.74429597339309 27.083548868015285 22.43286343681334 18-19 21.677690089637018 27.135469589574157 29.812419746114323 21.374420574674502 20-21 23.0259551072195 26.00358291446101 29.730275627195525 21.240186351123967 22-23 22.481321632359265 25.918355652280383 29.79143468579982 21.80888802956053 24-25 22.233606641867414 26.252338845408197 29.853315532407386 21.660738980317003 26-27 21.742911215473487 26.396749281535143 29.885086854876285 21.975252648115088 28-29 21.749294263694498 26.6880672328499 29.747377942766818 21.815260560688788 30-31 21.99656268280587 26.288385883171618 29.668730513589935 22.04632092043258 32-33 21.695094124946152 26.475235647311262 29.50100277644372 22.32866745129887 34-35 22.389862716362863 26.55474194329621 29.22523889700297 21.830156443337952 36-37 22.463942148431805 26.14140408644609 29.447521498893586 21.947132266228518 38-39 22.07100442282048 26.579482633919387 29.170535892825576 22.17897705043456 40-41 22.310937203810106 26.404307434040454 29.335723409028265 21.949031953121185 42-43 22.022741245365744 26.504377058376647 28.971344706026038 22.501536990231575 44-45 22.18286940809577 26.53793410699291 29.047966452301022 22.2312300326103 46-47 22.61664474357016 26.806957922973957 28.354451133852614 22.221946199603273 48-49 22.37065861841803 26.93220697602295 28.143791915948857 22.553342489610166 50-51 22.438063785791833 27.12192140764989 28.136601265513555 22.303413541044723 52-53 22.723877507080275 27.084209741797643 27.861052683880157 22.33086006724192 54-55 22.320470764951644 27.118212497562418 28.050269469296396 22.51104726818954 56-57 22.49976303621593 27.232002238428198 27.809159095208113 22.459075630147755 58-59 22.542482521317307 26.77527668114239 27.978142800206435 22.70409799733386 60-61 22.22219464565001 27.068708270613868 28.40483455918091 22.304262524555213 62-63 22.200861865553943 27.09913109711925 28.445785676142222 22.25422136118459 64-65 22.05029357934924 27.1816382473023 28.174093796324666 22.59397437702379 66-67 22.14013460528648 27.146917876860844 27.99221787305095 22.72072964480173 68-69 22.32520612773106 26.93164888516862 28.1656609651835 22.577484021916824 70-71 22.30540806944594 27.003238712135648 27.925401939914018 22.765951278504396 72-73 22.227380073403445 26.97951403655596 27.99497116411505 22.798134725925546 74-75 22.168708626755095 27.53401483794422 27.673035152634533 22.62424138266615 76-77 22.233331842850067 26.96267664296798 28.00696718121461 22.797024332967336 78-79 22.332392915741565 27.135766167152603 27.86131986284473 22.6705210542611 80-81 22.41069994552502 27.27097742020409 27.64352666707509 22.674795967195795 82-83 22.378404918947652 27.20511402307454 27.89913872048408 22.51734233749373 84-85 22.24389630002517 27.311490720256472 27.774981122577397 22.669631857140963 86-87 22.257424447345077 27.31540876324275 27.676731798372806 22.75043499103937 88-89 22.28529174553807 27.20686748444515 27.529722720430055 22.978118049586723 90-91 22.065041040934926 27.225200483993994 27.738470979822814 22.971287495248262 92-93 22.259161802817182 27.045529275445862 27.84776420289603 22.847544718840926 94-95 21.98728962179026 26.963733556615928 27.958898113651337 23.090078707942475 96-97 22.01250595275069 26.781373584280598 28.06178437584115 23.144336087127567 98-99 21.869030389239843 26.845146769862122 27.790594388436478 23.495228452461557 100-101 22.280713230569486 26.10086379925941 28.35620623869741 23.262216731473693 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1055.0 1 581.0 2 144.0 3 203.5 4 185.5 5 181.5 6 352.0 7 692.0 8 1253.5 9 1620.0 10 1465.5 11 1300.5 12 1369.0 13 1533.5 14 1684.0 15 1786.0 16 1863.0 17 1944.5 18 2056.0 19 2093.0 20 2224.0 21 2494.0 22 2857.0 23 3383.5 24 4023.5 25 5093.5 26 6712.0 27 8558.0 28 10306.5 29 12093.0 30 14265.0 31 17143.0 32 20540.5 33 23077.0 34 25139.0 35 27506.5 36 30623.5 37 33488.5 38 34960.0 39 36091.0 40 37228.0 41 38731.0 42 39392.5 43 40047.5 44 41672.0 45 43390.5 46 43711.0 47 44861.0 48 49024.0 49 48137.0 50 43760.5 51 41357.5 52 39566.0 53 39143.5 54 38692.0 55 36275.5 56 32822.0 57 29379.5 58 27228.5 59 24864.5 60 20491.0 61 16964.5 62 14121.0 63 10345.5 64 7211.0 65 5433.5 66 4005.0 67 3040.5 68 2382.0 69 1896.5 70 1461.0 71 991.0 72 736.5 73 595.5 74 461.0 75 348.5 76 252.0 77 168.0 78 118.0 79 82.0 80 54.0 81 40.5 82 31.0 83 20.5 84 17.5 85 13.5 86 5.0 87 5.0 88 5.5 89 6.0 90 4.0 91 3.5 92 5.0 93 3.0 94 1.0 95 1.5 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3970277285154046 2 0.11531925724513827 3 0.11655482071562188 4 0.12915756811455484 5 0.13154632415748985 6 0.0742161791270497 7 0.08377120329878973 8 0.08055873827553231 9 0.08187667264404816 10-11 0.07862302217177462 12-13 0.08335934880862851 14-15 0.07635782247588797 16-17 0.07845828037571013 18-19 0.08842515903761138 20-21 0.08583047574959576 22-23 0.09781544141328692 24-25 0.11120071234352617 26-27 0.11251864671204205 28-29 0.1054759349302854 30-31 0.09526194357428744 32-33 0.09377926740970709 34-35 0.3451340627550924 36-37 0.5368111424761186 38-39 0.519554439338364 40-41 0.49175426125248245 42-43 0.519224955746235 44-45 0.529397761653217 46-47 0.52639122387504 48-49 0.5329808957176193 50-51 0.531127550511894 52-53 0.5305509542256682 54-55 0.524661435016363 56-57 0.4967377005834331 58-59 0.4865237092274351 60-61 0.43372396358876825 62-63 0.5087226662471243 64-65 0.5363169170879252 66-67 0.5468603920360521 68-69 0.561810710028904 70-71 0.5560859326156632 72-73 0.5423711780932949 74-75 0.5472310610771971 76-77 0.5237965405870244 78-79 0.4980968204009651 80-81 0.5046864922435443 82-83 0.5175363523365741 84-85 0.513088323842833 86-87 0.5137884764761071 88-89 0.5237965405870244 90-91 0.5410120582757629 92-93 0.5472722465262133 94-95 0.5422064362972304 96-97 0.5433184434206657 98-99 0.5439362251559076 100-101 0.5216548972381861 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1214021.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.68655511134858 #Duplication Level Percentage of deduplicated Percentage of total 1 80.04185767038138 49.375064643987 2 12.045459266824997 14.860857738090093 3 3.4608797104496998 6.404692409772106 4 1.448058796534776 3.573030350276622 5 0.7874372958317749 2.428714707302904 6 0.47747069026363376 1.7672113229400777 7 0.32424701632659975 1.40011770096248 8 0.22946088182437574 1.13237210660464 9 0.1692749368326914 0.9397788947909883 >10 0.930185818714053 10.368581635883869 >50 0.05558226064377495 2.335285277004172 >100 0.02660910211987746 3.0663234435363025 >500 0.002540558145921184 0.9646422555570722 >1k 8.022815197645844E-4 0.9455061287260126 >5k 1.3371358662743075E-4 0.4378213845657921 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5308 0.43722472675513846 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4209 0.34669910981770496 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2197 0.18096886297683484 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1625 0.13385270930239263 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.237089803224161E-5 0.0 10-11 0.0 0.0 0.0 8.237089803224161E-5 8.237089803224161E-5 12-13 0.0 0.0 0.0 1.235563470483624E-4 1.6474179606448323E-4 14-15 0.0 0.0 0.0 1.6474179606448323E-4 2.0592724508060404E-4 16-17 0.0 0.0 0.0 1.6474179606448323E-4 2.471126940967248E-4 18-19 0.0 0.0 0.0 2.471126940967248E-4 2.471126940967248E-4 20-21 0.0 0.0 0.0 2.471126940967248E-4 2.471126940967248E-4 22-23 0.0 0.0 0.0 2.8829814311284567E-4 2.471126940967248E-4 24-25 0.0 0.0 0.0 6.58967184257933E-4 3.2948359212896645E-4 26-27 0.0 0.0 0.0 8.648944293385369E-4 3.2948359212896645E-4 28-29 0.0 0.0 0.0 0.001688603409660953 3.2948359212896645E-4 30-31 0.0 0.0 0.0 0.0034595777173541476 3.2948359212896645E-4 32-33 0.0 0.0 0.0 0.006877969985692175 3.2948359212896645E-4 34-35 0.0 0.0 0.0 0.013014601889094176 3.2948359212896645E-4 36-37 0.0 0.0 0.0 0.02487601120573697 3.2948359212896645E-4 38-39 0.0 0.0 0.0 0.04629244469411979 3.2948359212896645E-4 40-41 0.0 0.0 0.0 0.0678736199785671 3.2948359212896645E-4 42-43 0.0 0.0 0.0 0.09373808196069096 3.7066904114508724E-4 44-45 0.0 0.0 0.0 0.12660407027555537 4.118544901612081E-4 46-47 0.0 0.0 0.0 0.16840730102691798 4.118544901612081E-4 48-49 0.0 0.0 0.0 0.2141643348838282 4.118544901612081E-4 50-51 0.0 0.0 0.0 0.2701353600967364 4.118544901612081E-4 52-53 0.0 0.0 0.0 0.33240775900911107 5.765962862256913E-4 54-55 0.0 0.0 0.0 0.3985515901290011 5.765962862256913E-4 56-57 0.0 0.0 0.0 0.48178738259058124 5.765962862256913E-4 58-59 0.0 0.0 0.0 0.5756902063473366 5.765962862256913E-4 60-61 0.0 0.0 0.0 0.6869732895888951 5.765962862256913E-4 62-63 0.0 0.0 0.0 0.801139354261582 6.589671842579329E-4 64-65 0.0 0.0 0.0 0.9212361235925903 6.589671842579329E-4 66-67 0.0 0.0 0.0 1.0667031295175289 6.589671842579329E-4 68-69 0.0 0.0 0.0 1.2357282122796889 6.589671842579329E-4 70-71 0.0 0.0 0.0 1.4241104560794253 7.001526332740537E-4 72-73 0.0 8.237089803224161E-5 0.0 1.6299553302619971 7.413380822901746E-4 74-75 0.0 8.237089803224161E-5 0.0 1.8447374468810671 7.413380822901746E-4 76-77 0.0 8.237089803224161E-5 0.0 2.076776266637892 7.413380822901746E-4 78-79 0.0 8.237089803224161E-5 0.0 2.348353117450192 7.413380822901746E-4 80-81 0.0 8.237089803224161E-5 0.0 2.6481420008385355 8.237089803224162E-4 82-83 0.0 8.237089803224161E-5 0.0 2.968111754244778 9.884507763868993E-4 84-85 0.0 8.237089803224161E-5 0.0 3.30624429066713 9.884507763868993E-4 86-87 0.0 8.237089803224161E-5 0.0 3.667234751293429 9.884507763868993E-4 88-89 0.0 8.237089803224161E-5 0.0 4.055325237372335 9.884507763868993E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6260 0.0 29.04999 1 GTATCAA 9025 0.0 28.988514 1 GTGTAGC 785 0.0 24.799091 1 ATCAACG 10690 0.0 24.367584 3 TATCAAC 10675 0.0 24.311087 2 TCAACGC 10725 0.0 24.243902 4 CAACGCA 10805 0.0 24.020569 5 GAGTACT 4805 0.0 23.889828 12-13 GTACTTT 4850 0.0 23.473452 14-15 AACGCAG 11100 0.0 23.326046 6 ACGCAGA 12045 0.0 21.456686 7 CGCAGAG 12025 0.0 21.453009 8 GTATATG 505 0.0 20.684875 1 TAGACCG 115 0.0037868123 20.592054 4 AGTACTT 4975 0.0 20.31419 12-13 ACTTTTT 5700 0.0 20.181894 16-17 GCAGAGT 12575 0.0 20.100649 9 GTATAGC 620 0.0 19.14564 1 AGAGTAC 9790 0.0 18.953485 10-11 TACATGG 6980 0.0 18.86171 2 >>END_MODULE