FastQCFastQC Report
Fri 27 May 2016
SRR939134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214021
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53270.43878977381775114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43940.3619377259536697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22890.18854698559580108No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17240.14200742820758455No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12830.105681862175366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94150.028.6066061
GGTATCA66300.026.1713491
ATCAACG112050.023.92783
TCAACGC112600.023.8530654
TATCAAC112800.023.8107762
CAACGCA112950.023.7791525
GTAAGGT6450.023.542714
AACGCAG115650.023.2239996
GAGTACT53200.023.1468512-13
GTACTTT54350.022.43880714-15
TAAGGTG7150.021.9015125
GGTAAGG6950.021.1662083
AGTACTT55500.020.8195912-13
ACGCAGA129250.020.8170267
CGCAGAG130350.020.6413568
CTCCGAC2000.020.1676494-95
GCAGAGT136200.019.5108919
AGAGTAC105450.019.14781410-11
AGGTAAG7450.019.1087022
ACTTTTT64150.018.97391316-17
Produced by FastQC (version 0.11.5)