FastQCFastQC Report
Fri 27 May 2016
SRR939133_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939133_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805555
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83510.4625170653898663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64310.356178571131868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37700.20880006424617362No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23980.1328123485576457No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA136650.028.6929261
GGTATCA96200.026.1520231
ATCAACG164400.023.7431053
TCAACGC165250.023.5923124
TATCAAC167650.023.36762
CAACGCA166550.023.2943925
GAGTACT69600.022.99465612-13
AACGCAG169200.022.9766316
AGTACTT74450.021.9418812-13
GTACATG112750.021.8501931
TACATGG113100.021.3609092
GTACACG2005.424208E-721.3607731
GTACTTT76900.020.90532714-15
ACGCAGA187750.020.7065037
CGCAGAG187200.020.5902738
ACATGGG116800.019.5486323
GCAGAGT193800.019.4964949
TACTTTT86550.018.76595114-15
AGAGTAC160000.018.46596510-11
ACTTTTT92050.017.8004916-17
Produced by FastQC (version 0.11.5)