##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939133_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1805555 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08366402574278 33.0 31.0 34.0 30.0 34.0 2 32.19030990471074 34.0 31.0 34.0 30.0 34.0 3 32.2373691191905 34.0 31.0 34.0 30.0 34.0 4 35.68657393433044 37.0 35.0 37.0 35.0 37.0 5 35.740521335545026 37.0 35.0 37.0 35.0 37.0 6 35.82644726967608 37.0 36.0 37.0 35.0 37.0 7 35.75755931001825 37.0 36.0 37.0 35.0 37.0 8 35.752262877619344 37.0 36.0 37.0 35.0 37.0 9 37.42252991462459 39.0 38.0 39.0 35.0 39.0 10-11 37.33769838083027 39.0 37.0 39.0 34.5 39.0 12-13 37.2582236486842 39.0 37.0 39.0 34.0 39.0 14-15 38.53870222729299 40.0 38.0 41.0 34.0 41.0 16-17 38.47857916263975 40.0 38.0 41.0 34.0 41.0 18-19 38.451905092893874 40.0 38.0 41.0 34.0 41.0 20-21 38.37297756091617 40.0 38.0 41.0 34.0 41.0 22-23 38.293505044155395 40.0 38.0 41.0 34.0 41.0 24-25 38.322668930051975 40.0 38.0 41.0 34.0 41.0 26-27 38.24410084433872 40.0 38.0 41.0 34.0 41.0 28-29 38.121438837365794 40.0 38.0 41.0 33.5 41.0 30-31 37.94720099913877 40.0 38.0 41.0 33.0 41.0 32-33 37.73470096452337 40.0 37.5 41.0 33.0 41.0 34-35 37.429679516824464 40.0 37.0 41.0 32.5 41.0 36-37 37.1759220848991 40.0 37.0 41.0 31.0 41.0 38-39 36.94431019824929 40.0 36.5 41.0 30.5 41.0 40-41 36.7939423058284 40.0 36.0 41.0 30.5 41.0 42-43 36.59082636640811 40.0 36.0 41.0 30.0 41.0 44-45 36.29855833801795 40.0 36.0 41.0 30.0 41.0 46-47 35.89279667470667 39.0 35.0 41.0 28.5 41.0 48-49 35.826301331169645 39.5 35.5 41.0 27.5 41.0 50-51 34.829507547540786 38.5 34.0 40.0 25.5 40.5 52-53 34.98627236500688 38.5 35.0 40.0 26.0 40.5 54-55 35.498976215069604 39.0 35.0 41.0 27.0 41.0 56-57 35.40246738537458 39.0 35.0 41.0 26.0 41.0 58-59 35.23283810241172 39.0 35.0 41.0 26.0 41.0 60-61 34.959064110481265 39.0 35.0 41.0 26.0 41.0 62-63 34.609114372035194 38.0 34.5 40.0 25.5 41.0 64-65 34.2526442008136 37.5 34.0 40.0 23.5 41.0 66-67 33.86837288257627 37.0 34.0 40.0 22.5 41.0 68-69 33.4776135315734 36.5 34.0 39.0 22.0 41.0 70-71 33.08268648698046 36.0 33.0 39.0 21.5 41.0 72-73 32.639958073833256 35.5 33.0 39.0 20.0 40.0 74-75 32.18983553533401 35.0 32.5 37.5 18.5 40.0 76-77 31.754589862950727 35.0 32.0 37.0 17.0 39.0 78-79 31.334424041361245 35.0 32.0 36.5 12.0 39.0 80-81 30.915806497171232 35.0 31.0 36.0 7.5 38.5 82-83 30.501602277416083 35.0 31.0 36.0 6.5 37.0 84-85 30.132281209932678 34.5 31.0 35.0 2.0 37.0 86-87 29.76624195884368 34.0 30.5 35.0 2.0 36.5 88-89 29.38402845662414 34.0 30.0 35.0 2.0 36.0 90-91 29.033005640924813 34.0 29.5 35.0 2.0 36.0 92-93 28.653702878062425 34.0 29.0 35.0 2.0 35.0 94-95 28.232781887009814 34.0 29.0 35.0 2.0 35.0 96-97 27.710274403161353 34.0 28.5 35.0 2.0 35.0 98-99 27.06045481860148 34.0 27.0 35.0 2.0 35.0 100-101 24.62763305465632 31.5 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3044938392770504 1101 2 0.3470816546930635 1101 3 0.3837062264947235 1101 4 0.29626262023300143 1101 5 0.29311328347641563 1101 6 0.34935823774995356 1101 7 0.2703306738317508 1101 8 0.3418785038552201 1101 9 0.46214784214293303 1101 10-11 0.5392942129978522 1101 12-13 0.46248082258473033 1101 14-15 0.4905923485172323 1101 16-17 0.5981146794623768 1101 18-19 0.5631671760897987 1101 20-21 0.6289778688283505 1101 22-23 0.5862198134708692 1101 24-25 0.6025967925750848 1101 26-27 0.5004762216699277 1101 28-29 0.559752396993197 1101 30-31 0.5786588501513492 1101 32-33 0.5013187886488026 1101 34-35 0.5466044911126247 1101 36-37 0.38867938419894443 1101 38-39 0.45050980490928794 1101 40-41 0.3251788734592296 1101 42-43 0.24408427682249112 1101 44-45 0.21391943015603232 1101 46-47 0.21230558885963546 1101 48-49 0.29346366415418146 1101 50-51 0.2729046613567121 1101 52-53 0.4233766482954948 1101 54-55 0.3735351655832204 1101 56-57 0.33056710469293904 1101 58-59 0.39295770478862835 1101 60-61 0.3616514579923731 1101 62-63 0.4635154125121588 1101 64-65 0.36163081706576605 1101 66-67 0.2001650264276833 1101 68-69 0.33892543864396885 1101 70-71 0.25685557392210256 1101 72-73 0.18267806782944263 1101 74-75 0.07305741199394333 1101 76-77 0.09284881970364367 1101 78-79 0.004470373517666104 1101 80-81 -0.246470838194373 1101 82-83 -0.02253952482799093 1101 84-85 -0.3580626462869745 1101 86-87 -0.31103787059984356 1101 88-89 -0.21106492864663196 1101 90-91 -0.48889496050150427 1101 92-93 -0.5054966457911441 1101 94-95 -0.5418587573706759 1101 96-97 -0.3875330715818812 1101 98-99 -0.46995520396612633 1101 100-101 -0.7028274036677153 1102 1 0.3775780743176824 1102 2 0.45536178333487953 1102 3 0.4871694162414002 1102 4 0.4050631778920959 1102 5 0.42894208846999504 1102 6 0.4241621857228921 1102 7 0.40687794019318346 1102 8 0.4646070727942373 1102 9 0.4744527802776233 1102 10-11 0.40648540135037337 1102 12-13 0.5941188669756627 1102 14-15 0.7161389178047912 1102 16-17 0.7755528537625267 1102 18-19 0.7543919665715677 1102 20-21 0.7495997696220584 1102 22-23 0.7783052525481935 1102 24-25 0.6784099134064974 1102 26-27 0.6272284119077298 1102 28-29 0.7515661356855432 1102 30-31 0.6665511522378154 1102 32-33 0.6531307846564687 1102 34-35 0.8593387633833416 1102 36-37 0.7994424424521398 1102 38-39 0.7053188225103995 1102 40-41 0.6838542746727754 1102 42-43 0.6116426171529667 1102 44-45 0.7208727420490604 1102 46-47 0.8149584302277972 1102 48-49 0.9356647541242324 1102 50-51 0.9805498982826109 1102 52-53 1.0233144661374212 1102 54-55 1.1125863529851898 1102 56-57 1.001959351330349 1102 58-59 0.9544713056504648 1102 60-61 1.1087217673981158 1102 62-63 1.0275800661139698 1102 64-65 1.0091439268073756 1102 66-67 0.9654685704146289 1102 68-69 0.8550821059971483 1102 70-71 0.7320298423906095 1102 72-73 0.7613701829733728 1102 74-75 0.8076600006728327 1102 76-77 0.6646208923894363 1102 78-79 0.7656734596538151 1102 80-81 0.8569551214703957 1102 82-83 0.7026208813759673 1102 84-85 0.6610007399077951 1102 86-87 0.6299433400907297 1102 88-89 0.6640039866113625 1102 90-91 0.49089663025275954 1102 92-93 0.5222033272764541 1102 94-95 0.26921996444932006 1102 96-97 0.38737108076044535 1102 98-99 0.013450477185948273 1102 100-101 -0.2274103475374254 1103 1 0.27939609608036164 1103 2 0.3443545227300717 1103 3 0.4016600065434943 1103 4 0.3455484174690895 1103 5 0.36963362567437485 1103 6 0.372855315131595 1103 7 0.3355069986875563 1103 8 0.3640536286955367 1103 9 0.49767217148912124 1103 10-11 0.4731859406330443 1103 12-13 0.5238354852255398 1103 14-15 0.5157975124548315 1103 16-17 0.5657171646557302 1103 18-19 0.5519625153844956 1103 20-21 0.5233019891395756 1103 22-23 0.6565184624856855 1103 24-25 0.5478470538687787 1103 26-27 0.5034578730460737 1103 28-29 0.46699023232010006 1103 30-31 0.5595747329369303 1103 32-33 0.6138657247382966 1103 34-35 0.5412431316112176 1103 36-37 0.4324770461042604 1103 38-39 0.5979839658661987 1103 40-41 0.4645258327748323 1103 42-43 0.3958920420839718 1103 44-45 0.3847358277869688 1103 46-47 0.4419575843692485 1103 48-49 0.5684230184684296 1103 50-51 0.5617745177419522 1103 52-53 0.5632181301210721 1103 54-55 0.6390366559250253 1103 56-57 0.3625978405734074 1103 58-59 0.42367229465430967 1103 60-61 0.5234764492825903 1103 62-63 0.5032756029662266 1103 64-65 0.3567523670212509 1103 66-67 0.28441675870810457 1103 68-69 0.24753942075986402 1103 70-71 0.29238280653156323 1103 72-73 0.22755574367042186 1103 74-75 0.26380567971352775 1103 76-77 0.3602306568886391 1103 78-79 0.17927972589640717 1103 80-81 0.20222462884391135 1103 82-83 -0.011892290670129313 1103 84-85 0.21110082679009778 1103 86-87 0.09818192578462615 1103 88-89 -0.026429965614852335 1103 90-91 -0.02984355338657707 1103 92-93 -0.04273583597424491 1103 94-95 -0.014277756325498103 1103 96-97 -0.1694621932479663 1103 98-99 -0.43307021190171113 1103 100-101 -0.5140436774260841 1104 1 0.15025515865387717 1104 2 0.1736928401847848 1104 3 0.29930688374885506 1104 4 0.24599941429337235 1104 5 0.24118259944525278 1104 6 0.26091952061266 1104 7 0.1689355302095521 1104 8 0.23804908564684268 1104 9 0.17174040543407187 1104 10-11 0.28374558836299 1104 12-13 0.21388433402582052 1104 14-15 0.27994087578110793 1104 16-17 0.40753912566490413 1104 18-19 0.4055311609972918 1104 20-21 0.4330750572412043 1104 22-23 0.38927178523328365 1104 24-25 0.36854053040370616 1104 26-27 0.3742142024391555 1104 28-29 0.4271916922707035 1104 30-31 0.3849141751270011 1104 32-33 0.45300908806458295 1104 34-35 0.21807707509510266 1104 36-37 0.3352120643352663 1104 38-39 0.4736643204886377 1104 40-41 0.2601320136734486 1104 42-43 0.2245560696657236 1104 44-45 0.38029753385612963 1104 46-47 0.4004164396385974 1104 48-49 0.40290332457286837 1104 50-51 0.3834694130607019 1104 52-53 0.4202333912361027 1104 54-55 0.46248812322684074 1104 56-57 0.3171230625799879 1104 58-59 0.35423219967665176 1104 60-61 0.3669513301929612 1104 62-63 0.4431336878286132 1104 64-65 0.4046322776305189 1104 66-67 0.3903434891832802 1104 68-69 0.3829873300753164 1104 70-71 0.34401477548004067 1104 72-73 0.37558672167560303 1104 74-75 0.41130443000757566 1104 76-77 0.49783375762963544 1104 78-79 0.369481479895466 1104 80-81 0.23466809802762967 1104 82-83 0.3374942507318899 1104 84-85 0.3767102054657059 1104 86-87 0.34273449429791825 1104 88-89 0.4064314199414909 1104 90-91 0.3301950772297495 1104 92-93 0.12124690338120203 1104 94-95 0.09685381565756401 1104 96-97 -0.048860055515667966 1104 98-99 -0.26006900630303065 1104 100-101 -0.32060977951971026 1105 1 0.23070458570175134 1105 2 0.23399215094040215 1105 3 0.22115068338580102 1105 4 0.17952314075127873 1105 5 0.19928370280354812 1105 6 0.25488827770364963 1105 7 0.1971183217911303 1105 8 0.24983750463935905 1105 9 0.26015762519808305 1105 10-11 0.27281517935459476 1105 12-13 0.3379027635130001 1105 14-15 0.41839284140397837 1105 16-17 0.32201875324602725 1105 18-19 0.2885975184967222 1105 20-21 0.29696170064941185 1105 22-23 0.3846151310467576 1105 24-25 0.40032465934630324 1105 26-27 0.3181015904014899 1105 28-29 0.4621486854066248 1105 30-31 0.27352834718282537 1105 32-33 0.11882093569334984 1105 34-35 0.2329754257304515 1105 36-37 0.0670119707341712 1105 38-39 0.32754202924743225 1105 40-41 0.21360163586540892 1105 42-43 0.044418468973645986 1105 44-45 -0.01037391213888128 1105 46-47 0.22839908798214026 1105 48-49 0.4252333889590787 1105 50-51 0.22916376295858498 1105 52-53 0.28943934199624977 1105 54-55 0.40191722749278114 1105 56-57 0.2988972641343324 1105 58-59 0.17646653567619808 1105 60-61 0.2689150030429701 1105 62-63 0.14370752632418515 1105 64-65 0.270053766171479 1105 66-67 0.30602645803964634 1105 68-69 0.19397659431403014 1105 70-71 0.07200717393704537 1105 72-73 0.15753057520407765 1105 74-75 0.19558831076546568 1105 76-77 0.25173291663803354 1105 78-79 0.06001908342394202 1105 80-81 -0.06906708179991838 1105 82-83 -0.009700198439880126 1105 84-85 0.07593533735814617 1105 86-87 -0.005314596636960545 1105 88-89 -0.05959280288583102 1105 90-91 0.026880215929544704 1105 92-93 0.04985976360357469 1105 94-95 -0.013476695461111632 1105 96-97 0.3519174717137368 1105 98-99 0.2367790479288736 1105 100-101 0.15285572989713003 1106 1 0.20258482579647819 1106 2 0.18771574086299125 1106 3 0.2553265383981511 1106 4 0.2788068946610309 1106 5 0.3264285056562102 1106 6 0.2375796245748205 1106 7 0.29215216638660024 1106 8 0.2473089129714907 1106 9 0.25002854348511505 1106 10-11 0.2270390806885345 1106 12-13 0.23850214479683274 1106 14-15 0.28751076021801936 1106 16-17 0.3863221158041128 1106 18-19 0.27408232778294206 1106 20-21 0.2757472306304578 1106 22-23 0.32729884662895614 1106 24-25 0.3129766862577128 1106 26-27 0.3339448991641305 1106 28-29 0.3289790996863715 1106 30-31 0.27275118766493023 1106 32-33 0.38169266159660964 1106 34-35 0.3450354288787878 1106 36-37 0.38879245653587446 1106 38-39 0.5757873460999221 1106 40-41 0.5518646792097641 1106 42-43 0.5644820858624016 1106 44-45 0.5433226431725657 1106 46-47 0.646942435756749 1106 48-49 0.7072680343265745 1106 50-51 0.6212438196286101 1106 52-53 0.59919165020451 1106 54-55 0.6505249450187378 1106 56-57 0.5965639034241832 1106 58-59 0.6388335014346325 1106 60-61 0.7305881374837 1106 62-63 0.7227976234434621 1106 64-65 0.6532825542680385 1106 66-67 0.6315110277786715 1106 68-69 0.6402867121027995 1106 70-71 0.4506652305933798 1106 72-73 0.42948781260902535 1106 74-75 0.4548204593668501 1106 76-77 0.4261053030071196 1106 78-79 0.4584844414785465 1106 80-81 0.5451170204798892 1106 82-83 0.6038819559790802 1106 84-85 0.6803400968185507 1106 86-87 0.5300316174908453 1106 88-89 0.6102117608302962 1106 90-91 0.5955921022609836 1106 92-93 0.7097681196938872 1106 94-95 0.5174976113919918 1106 96-97 0.6242851903347706 1106 98-99 0.5453337913053957 1106 100-101 0.5449867758970335 1107 1 0.13136232531337555 1107 2 0.16220537135819768 1107 3 0.09725951628959706 1107 4 0.16513498369182855 1107 5 0.1533820850839902 1107 6 0.09155350984170951 1107 7 0.11814537831980232 1107 8 0.2067046920234219 1107 9 0.2884824750260222 1107 10-11 0.21540897196792486 1107 12-13 0.2590691506939251 1107 14-15 0.26644460315350926 1107 16-17 0.3330583577778228 1107 18-19 0.28607687035361806 1107 20-21 0.21245230118925917 1107 22-23 0.3265023770980875 1107 24-25 0.30580521818588124 1107 26-27 0.20730964816517172 1107 28-29 0.2449221403615951 1107 30-31 0.21439410993158248 1107 32-33 0.36020873494508265 1107 34-35 0.32418407379442016 1107 36-37 0.4190957472049419 1107 38-39 0.5065505886014705 1107 40-41 0.4138108791763031 1107 42-43 0.35972521900785637 1107 44-45 0.36410516435447704 1107 46-47 0.485602317815065 1107 48-49 0.47117326077487576 1107 50-51 0.4156371475062386 1107 52-53 0.47851087220957567 1107 54-55 0.6010146286875653 1107 56-57 0.6500791013532634 1107 58-59 0.5367976974300177 1107 60-61 0.3107395915228821 1107 62-63 0.4508435641561661 1107 64-65 0.43725417641368125 1107 66-67 0.32412031578387257 1107 68-69 0.5613506319844177 1107 70-71 0.484765409697701 1107 72-73 0.602256044352643 1107 74-75 0.7351138610390962 1107 76-77 0.6116544957407513 1107 78-79 0.604576861124773 1107 80-81 0.5611003952929714 1107 82-83 0.5533415628314735 1107 84-85 0.5582104997319455 1107 86-87 0.524447090236773 1107 88-89 0.5090228440288627 1107 90-91 0.42725170937276147 1107 92-93 0.48552230971047905 1107 94-95 0.24615731836642496 1107 96-97 0.357841085538638 1107 98-99 0.4074994945784276 1107 100-101 0.5730324572262155 1108 1 -0.026294282758371423 1108 2 0.04938174731424283 1108 3 -0.029361779904562013 1108 4 0.10629517798090404 1108 5 0.07206722548993838 1108 6 0.08211947157632693 1108 7 0.17613774110841263 1108 8 0.18201575160284023 1108 9 0.23969702960258843 1108 10-11 0.152678675093334 1108 12-13 0.2705339534612605 1108 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2316 50-51 -1.9473441282367148 2316 52-53 -2.0572845545278113 2316 54-55 -1.9019067057345183 2316 56-57 -1.8667437510499312 2316 58-59 -1.815535557767518 2316 60-61 -1.697569506832913 2316 62-63 -2.0038313179317555 2316 64-65 -2.0756628304871683 2316 66-67 -2.1309540892463517 2316 68-69 -2.100479857978563 2316 70-71 -1.8408709033572492 2316 72-73 -1.7220454344747047 2316 74-75 -1.8475515829310964 2316 76-77 -1.8550993223951693 2316 78-79 -1.6725523179599016 2316 80-81 -1.5008983012988502 2316 82-83 -1.5259257466094773 2316 84-85 -1.5898511313829324 2316 86-87 -1.6148805469108787 2316 88-89 -1.6282570770453937 2316 90-91 -1.5701774081477744 2316 92-93 -1.795842559575199 2316 94-95 -1.8246739005778139 2316 96-97 -1.9521994076957476 2316 98-99 -1.978261801411147 2316 100-101 -1.8087744374424304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5474.0 3 1347.0 4 1343.0 5 1454.0 6 1754.0 7 2147.0 8 2659.0 9 3166.0 10 3887.0 11 4878.0 12 6607.0 13 10165.0 14 9299.0 15 10117.0 16 12908.0 17 18100.0 18 16994.0 19 10060.0 20 9515.0 21 9979.0 22 10448.0 23 11617.0 24 12839.0 25 14462.0 26 16869.0 27 19467.0 28 23218.0 29 27541.0 30 32871.0 31 40502.0 32 51175.0 33 67098.0 34 93855.0 35 138283.0 36 225637.0 37 383288.0 38 413343.0 39 81174.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.64971727865072 19.912863474068825 15.78849386626655 21.648925381013907 2 14.983494982507953 21.245697688410335 40.176922253687636 23.593885075394077 3 18.63433074987552 26.13142255781175 30.61281068307305 24.621436009239677 4 12.658495280492147 17.836645472965554 38.292171417227564 31.21268782931474 5 13.482931699065473 38.066110202157454 33.70871578713846 14.742242311638613 6 29.792527256157996 36.9813458566427 18.31655946022053 14.909567426978773 7 27.05296414377526 34.111061772005655 21.26737193868868 17.568602145530406 8 26.202980569913 34.17554216246273 20.420302381318024 19.201174886306244 9 26.832250897471997 17.1778579965365 21.079061795298777 34.91082931069272 10-11 24.50490706795726 27.352444969579754 27.16499827187615 20.977649690586837 12-13 25.94060904335081 24.942222759326818 27.784824195872194 21.33234400145018 14-15 23.095176455653117 25.861517472604902 26.96890759423709 24.07439847750489 16-17 21.451743028951217 29.214480569529233 27.329537691235977 22.00423871028357 18-19 21.465495185616472 27.503619415200077 30.20556171688481 20.82532368229864 20-21 22.88547214778152 26.346176929873028 29.919792552320025 20.84855837002543 22-23 22.24843081154213 26.51676203783796 29.818933395435494 21.41587375518442 24-25 21.887935050242895 26.698830993101307 30.008512455358133 21.404721501297665 26-27 21.642941949909414 26.794548518704808 30.076561636109854 21.485947895275924 28-29 21.41112758673083 27.039883815457777 30.13206620167711 21.41692239613428 30-31 21.731660707458754 26.73709039282533 29.970199801886487 21.56104909782943 32-33 21.545615054959953 26.79984797338037 29.79541552695215 21.859121444707526 34-35 22.09196941576555 26.85039063802358 29.625420922194685 21.43221902401618 36-37 22.10266838052425 26.51341203015595 29.777960084631687 21.60595950468811 38-39 21.768310800359224 26.826851277919538 29.69152879617275 21.713309125548484 40-41 21.86981426912869 26.7685932528965 29.670933649903397 21.690658828071413 42-43 21.752177429743927 26.926562403051058 29.291706165027513 22.0295540021775 44-45 21.8336311139518 27.01718427440078 29.112815956551945 22.03636865509547 46-47 22.10647059511042 27.271910144877527 28.59648956816134 22.025129691850715 48-49 22.06401771840965 27.21074481944416 28.626124690691796 22.09911277145439 50-51 22.094903896725505 27.472513099932378 28.45934220668813 21.973240796653986 52-53 22.224572203004943 27.58129464770232 28.081376742425718 22.112756406867014 54-55 21.875915615740368 27.47101819670202 28.317815805868456 22.33525038168916 56-57 22.092398304391633 27.627633809571762 28.02608174594917 22.253886140087427 58-59 22.19332731639412 27.23517934697204 28.19451494337912 22.376978393254724 60-61 22.168316955554104 27.29222415756031 28.525916462900124 22.013542423985466 62-63 22.09385532307004 27.23733535727978 28.409191447119852 22.259617872530324 64-65 21.80514803186816 27.274455294322937 28.45307734460209 22.467319329206813 66-67 21.804123080074636 27.404471327481705 28.183324204085448 22.608081388358205 68-69 21.91614336182038 27.274410121609744 28.35210401534576 22.457342501224115 70-71 21.89368728226585 27.27645960513087 28.24960391729415 22.580249195309136 72-73 21.93601518949716 27.21125942072508 28.200235512908538 22.652489876869218 74-75 21.735518074381734 27.634892284072006 28.013934780085485 22.615654861460772 76-77 21.834628738487517 27.241492442534632 28.15077586564333 22.773102953334526 78-79 21.849166397265968 27.32602232696546 28.195625526165998 22.629185749602577 80-81 21.71871174519798 27.535027487814105 28.076325980992234 22.669934785995682 82-83 21.754550673115904 27.45897180401073 28.039275320419087 22.747202202454275 84-85 21.754122354486313 27.31077238147709 28.03134269384666 22.903762570189937 86-87 21.723851319068963 27.289493946226585 28.017989213929116 22.968665520775332 88-89 21.779407622955475 27.107060679295525 28.058121167121037 23.055410530627967 90-91 21.662913654747026 27.021685821408592 28.25892720455817 23.056473319286212 92-93 21.797185091544783 26.91775345044631 28.24457735320452 23.040484104804392 94-95 21.55655305574351 26.874972688120746 28.284279574555377 23.284194681580367 96-97 21.58551096155211 26.730266353260774 28.336875153954384 23.347347531232735 98-99 21.469546090759582 26.655955059119336 28.085462040358067 23.78903680976302 100-101 21.667605868970842 26.31152296871413 28.585811783858244 23.435059378456785 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1792.0 1 1046.5 2 314.0 3 336.0 4 319.0 5 331.5 6 598.0 7 1214.5 8 1994.5 9 2481.5 10 2454.0 11 2399.5 12 2555.5 13 2758.5 14 2916.5 15 3022.5 16 3240.0 17 3419.5 18 3498.5 19 3637.0 20 3821.5 21 4209.0 22 4835.5 23 5716.0 24 6803.0 25 8430.0 26 10965.5 27 13756.5 28 15829.0 29 18418.5 30 22325.5 31 26431.5 32 30673.0 33 34466.0 34 38514.5 35 41889.5 36 44894.5 37 49294.0 38 52047.5 39 52941.0 40 54210.5 41 57392.5 42 60163.0 43 62309.0 44 65071.0 45 66648.5 46 66687.0 47 68532.5 48 77235.0 49 77687.5 50 68859.0 51 63509.5 52 59674.0 53 57536.0 54 54801.5 55 49404.0 56 43722.5 57 39423.5 58 35841.0 59 31870.0 60 27052.0 61 22035.5 62 17840.0 63 13404.0 64 9423.5 65 7162.0 66 5403.0 67 4186.5 68 3128.5 69 2264.0 70 1620.5 71 1205.0 72 1078.5 73 953.0 74 730.0 75 568.0 76 412.5 77 252.0 78 155.0 79 104.5 80 78.0 81 53.0 82 40.0 83 32.5 84 18.5 85 18.0 86 16.5 87 9.5 88 6.5 89 5.5 90 4.0 91 3.5 92 3.0 93 3.5 94 3.5 95 3.0 96 2.5 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33673856515032774 2 0.11985234456995217 3 0.11237541919243668 4 0.12123696038060319 5 0.13873850422723208 6 0.08634464195219753 7 0.09481849071338175 8 0.10002464616142959 9 0.08856002724923916 10-11 0.08725848838722719 12-13 0.091052335708411 14-15 0.0927138746811922 16-17 0.08133233271764084 18-19 0.09645233736994996 20-21 0.10631080194178522 22-23 0.11492311228403454 24-25 0.12821542406628433 26-27 0.1274954238447458 28-29 0.12278773008853235 30-31 0.11317849636261426 32-33 0.10653234047148938 34-35 0.32870779344855183 36-37 0.4889632273733007 38-39 0.46174168053590176 40-41 0.44052936631672807 42-43 0.47295706860217496 44-45 0.47910476280146547 46-47 0.48716322681945434 48-49 0.49876630731271 50-51 0.48683091902489817 52-53 0.49015399697046064 54-55 0.47677860823957174 56-57 0.4544862936880904 58-59 0.43490782612548495 60-61 0.3950308907787356 62-63 0.46556322017329854 64-65 0.49655092201566825 66-67 0.505717078682178 68-69 0.5183170825591024 70-71 0.5102586185411133 72-73 0.5012586157718818 74-75 0.5087909257818233 76-77 0.4849201492061998 78-79 0.4627939885519965 80-81 0.4649539892166121 82-83 0.47910476280146547 84-85 0.47880014732312226 86-87 0.4818739944227675 88-89 0.49452938293211784 90-91 0.5106463109680957 92-93 0.5031416932743672 94-95 0.5081816948251369 96-97 0.5074616946035984 98-99 0.5018678467285682 100-101 0.4883539964166143 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1805555.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.49962586105092 #Duplication Level Percentage of deduplicated Percentage of total 1 72.5824810721504 37.379706192825566 2 14.643891368776115 15.083098532836859 3 5.354729815979436 8.272997463298651 4 2.4751289671018153 5.098728630543717 5 1.3178673473551599 3.393483766164319 6 0.8270136316978887 2.5554535568658143 7 0.5552046258853469 2.0014981356594084 8 0.3822048585785012 1.5746725775254953 9 0.28152036023440846 1.3048373901906285 >10 1.43036294848634 13.311624933127794 >50 0.09939621561844444 3.4836672807942413 >100 0.04706857233152181 4.285307917802912 >500 0.0021587695204549904 0.7132484900421698 >1k 7.555693321592467E-4 0.719974627310902 >5k 2.1587695204549904E-4 0.8217005050115623 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8351 0.4625170653898663 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6431 0.356178571131868 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3770 0.20880006424617362 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2398 0.1328123485576457 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.1076926485208149E-4 0.0 5 0.0 0.0 0.0 1.1076926485208149E-4 0.0 6 0.0 0.0 0.0 1.1076926485208149E-4 0.0 7 1.1076926485208149E-4 0.0 0.0 1.1076926485208149E-4 0.0 8 1.1076926485208149E-4 0.0 0.0 1.1076926485208149E-4 0.0 9 1.1076926485208149E-4 0.0 0.0 2.2153852970416298E-4 0.0 10-11 1.1076926485208149E-4 0.0 0.0 2.2153852970416298E-4 0.0 12-13 1.1076926485208149E-4 0.0 0.0 2.7692316213020373E-4 0.0 14-15 1.6615389727812224E-4 0.0 0.0 3.600001107692648E-4 0.0 16-17 1.938462134911426E-4 0.0 0.0 3.876924269822852E-4 0.0 18-19 2.2153852970416298E-4 0.0 0.0 3.876924269822852E-4 0.0 20-21 2.2153852970416298E-4 0.0 0.0 4.153847431953056E-4 0.0 22-23 2.2153852970416298E-4 0.0 0.0 9.415387512426927E-4 0.0 24-25 2.7692316213020373E-4 0.0 0.0 0.001329231178224978 0.0 26-27 2.7692316213020373E-4 0.0 0.0 0.0018276928700593446 0.0 28-29 2.7692316213020373E-4 0.0 0.0 0.004320001329231178 0.0 30-31 2.7692316213020373E-4 0.0 0.0 0.01121538806627325 0.0 32-33 3.0461547834322413E-4 0.0 0.0 0.02157231432994287 0.0 34-35 3.323077945562445E-4 0.0 0.0 0.03752308846864261 0.0 36-37 3.876924269822852E-4 0.0 0.0 0.06241848074414792 0.0 38-39 3.876924269822852E-4 0.0 0.0 0.10057849248569 0.0 40-41 4.153847431953056E-4 0.0 0.0 0.14535696780214394 0.0 42-43 4.7076937562134637E-4 0.0 0.0 0.19797236860688266 0.0 44-45 5.538463242604075E-4 0.0 0.0 0.2631877732885456 0.0 46-47 7.476925377515501E-4 0.0 0.0 0.3424154899739969 0.0 48-49 8.307694863906112E-4 0.0 0.0 0.4306985940611059 0.0 50-51 8.307694863906112E-4 0.0 0.0 0.5303909324279792 0.0 52-53 8.307694863906112E-4 0.0 0.0 0.6300555784786395 0.0 54-55 8.307694863906112E-4 0.0 0.0 0.7409356125955731 0.0 56-57 8.307694863906112E-4 0.0 0.0 0.8808648864199651 0.0 58-59 8.307694863906112E-4 0.0 0.0 1.0362741650074354 0.0 60-61 8.307694863906112E-4 0.0 0.0 1.2136988349842568 0.0 62-63 8.307694863906112E-4 0.0 0.0 1.3979635070656946 0.0 64-65 8.307694863906112E-4 0.0 0.0 1.5961020295698551 0.0 66-67 9.415387512426927E-4 0.0 0.0 1.8210744064844326 0.0 68-69 9.415387512426927E-4 0.0 0.0 2.0855637186349902 0.0 70-71 9.969233836687335E-4 0.0 0.0 2.3763607311879174 0.0 72-73 0.0010523080160947742 0.0 0.0 2.6813085173256974 0.0 74-75 0.0010523080160947742 0.0 0.0 3.0043116936343672 0.0 76-77 0.0010523080160947742 0.0 0.0 3.348914876589193 0.0 78-79 0.001107692648520815 0.0 0.0 3.7439180750517154 0.0 80-81 0.0012738465457989372 0.0 0.0 4.165505897078738 0.0 82-83 0.0012738465457989372 0.0 0.0 4.6215983451071825 0.0 84-85 0.0013015388620119576 0.0 0.0 5.0847523337699485 0.0 86-87 0.001329231178224978 0.0 0.0 5.579087870488575 0.0 88-89 0.001329231178224978 0.0 0.0 6.126315731174071 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13665 0.0 28.692926 1 GGTATCA 9620 0.0 26.152023 1 ATCAACG 16440 0.0 23.743105 3 TCAACGC 16525 0.0 23.592312 4 TATCAAC 16765 0.0 23.3676 2 CAACGCA 16655 0.0 23.294392 5 GAGTACT 6960 0.0 22.994656 12-13 AACGCAG 16920 0.0 22.976631 6 AGTACTT 7445 0.0 21.94188 12-13 GTACATG 11275 0.0 21.850193 1 TACATGG 11310 0.0 21.360909 2 GTACACG 200 5.424208E-7 21.360773 1 GTACTTT 7690 0.0 20.905327 14-15 ACGCAGA 18775 0.0 20.706503 7 CGCAGAG 18720 0.0 20.590273 8 ACATGGG 11680 0.0 19.548632 3 GCAGAGT 19380 0.0 19.496494 9 TACTTTT 8655 0.0 18.765951 14-15 AGAGTAC 16000 0.0 18.465965 10-11 ACTTTTT 9205 0.0 17.80049 16-17 >>END_MODULE