FastQCFastQC Report
Fri 27 May 2016
SRR939133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805555
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82240.4554832170717591No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64940.35966780297470863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38560.2135631426348131No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23540.1303754247308999No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102650.027.6018071
CCGTCGA350.00842908927.11959330-31
GTATCAA137950.025.2678851
GAGTACT74200.023.08491512-13
GTACTTT79700.021.34301814-15
GTACATG111300.021.0498711
ATCAACG166200.020.9510523
TCAACGC167650.020.826444
CAACGCA167650.020.7698485
TATCAAC169150.020.7539352
TACATGG110000.020.744032
AACGCAG171900.020.3391326
AGTACTT78600.019.80050712-13
ACATGGG114100.019.3333953
CATGGGG42300.018.95344
ACTTTTT92700.018.47533416-17
CGCAGAG189700.018.3056248
ACGCAGA190000.018.301697
GCAGAGT197250.017.2696829
AGAGTAC160600.017.254110-11
Produced by FastQC (version 0.11.5)