##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939133_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1805555 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.509496525998934 34.0 31.0 34.0 31.0 34.0 2 32.719240344381646 34.0 31.0 34.0 31.0 34.0 3 32.79718424528746 34.0 31.0 34.0 31.0 34.0 4 36.26227226531454 37.0 35.0 37.0 35.0 37.0 5 36.22915557820172 37.0 37.0 37.0 35.0 37.0 6 36.265316758559 37.0 37.0 37.0 35.0 37.0 7 36.22014948312292 37.0 37.0 37.0 35.0 37.0 8 36.20917335666873 37.0 37.0 37.0 35.0 37.0 9 37.9464607835264 39.0 38.0 39.0 35.0 39.0 10-11 37.896187875750115 39.0 38.0 39.0 35.0 39.0 12-13 37.831877179039125 39.0 38.0 39.0 35.0 39.0 14-15 39.181312117326804 40.5 39.0 41.0 36.0 41.0 16-17 39.13205967140298 40.0 38.5 41.0 36.0 41.0 18-19 39.1872053191401 40.5 38.5 41.0 36.0 41.0 20-21 39.25817158712972 41.0 39.0 41.0 36.0 41.0 22-23 39.21943751367308 41.0 39.0 41.0 36.0 41.0 24-25 39.16996657537433 41.0 39.0 41.0 36.0 41.0 26-27 39.03821567329713 40.5 39.0 41.0 35.0 41.0 28-29 38.894913752281155 40.0 38.0 41.0 35.0 41.0 30-31 38.69816261481927 40.0 38.0 41.0 35.0 41.0 32-33 38.54334152102816 40.0 38.0 41.0 34.0 41.0 34-35 38.49058876633501 40.0 38.0 41.0 34.0 41.0 36-37 38.38570024175392 40.0 38.0 41.0 34.0 41.0 38-39 38.25503017077851 40.0 38.0 41.0 34.0 41.0 40-41 38.08589630335271 40.0 38.0 41.0 33.5 41.0 42-43 37.91377415808436 40.0 38.0 41.0 33.0 41.0 44-45 37.66550894323352 40.0 37.5 41.0 33.0 41.0 46-47 37.33782161163742 40.0 37.0 41.0 32.5 41.0 48-49 36.99786464549682 40.0 37.0 41.0 31.5 41.0 50-51 36.74902592277721 40.0 36.5 41.0 31.0 41.0 52-53 36.52337342257644 40.0 36.0 41.0 31.0 41.0 54-55 36.29595526029392 39.0 36.0 41.0 30.0 41.0 56-57 36.04238835150411 39.0 35.0 41.0 29.5 41.0 58-59 35.76679331285948 39.0 35.0 40.0 29.0 41.0 60-61 35.432959394756736 38.0 35.0 40.0 28.0 41.0 62-63 35.06225038838473 38.0 34.5 40.0 28.0 41.0 64-65 34.67777027008316 37.5 34.0 40.0 27.0 41.0 66-67 34.32786899319046 37.0 34.0 39.5 26.5 41.0 68-69 33.87801894708275 36.0 34.0 39.0 26.0 40.5 70-71 33.4685276826239 36.0 33.0 39.0 26.0 40.0 72-73 33.026165638820196 35.0 33.0 38.0 25.0 40.0 74-75 32.548621614960496 35.0 32.5 37.0 23.5 39.5 76-77 30.562173680668824 33.5 30.0 35.5 20.0 38.5 78-79 31.521066098789568 34.5 31.5 36.0 21.0 39.0 80-81 31.55219946221522 35.0 32.0 36.0 22.0 37.5 82-83 31.271416545051245 35.0 32.0 35.5 20.5 37.0 84-85 30.90695990983382 34.5 31.5 35.0 19.5 37.0 86-87 30.378042208628372 34.0 31.0 35.0 15.5 36.0 88-89 29.85725386377042 34.0 30.5 35.0 7.0 36.0 90-91 29.444339275181314 34.0 30.0 35.0 4.5 35.5 92-93 29.188925842746414 34.0 30.0 35.0 2.0 35.0 94-95 28.90579184793595 34.0 29.5 35.0 2.0 35.0 96-97 28.41186588057412 34.0 29.0 35.0 2.0 35.0 98-99 27.71109908033818 34.0 29.0 35.0 2.0 35.0 100-101 24.200137353888415 30.0 14.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16847605059692228 1101 2 0.16136416561915468 1101 3 0.19879444349602693 1101 4 0.16804914286679917 1101 5 0.1251468474354951 1101 6 0.21164424727892595 1101 7 0.17911241115873366 1101 8 0.1674964777447343 1101 9 0.18258211929254742 1101 10-11 0.21276946102872074 1101 12-13 0.2753632006320501 1101 14-15 0.3418301532079795 1101 16-17 0.31439812625787766 1101 18-19 0.36793032840455453 1101 20-21 0.3470833109421747 1101 22-23 0.3076705370670041 1101 24-25 0.27902169876919203 1101 26-27 0.31843786865341883 1101 28-29 0.3172146767601802 1101 30-31 0.2965734188846554 1101 32-33 0.35057548454637555 1101 34-35 0.41134753175626315 1101 36-37 0.4396847814280349 1101 38-39 0.5000203711701587 1101 40-41 0.5164729377371913 1101 42-43 0.4687481733789056 1101 44-45 0.6427375163710565 1101 46-47 0.672382703801297 1101 48-49 0.8813188910785996 1101 50-51 0.8553751774779883 1101 52-53 0.8706030365119588 1101 54-55 0.7995696441204885 1101 56-57 0.7279574066769499 1101 58-59 0.7256926872009686 1101 60-61 0.711252301286855 1101 62-63 0.8432943263129502 1101 64-65 0.8615950880774221 1101 66-67 0.784460784568914 1101 68-69 0.667287949101059 1101 70-71 0.7317524579195975 1101 72-73 0.7221016198862387 1101 74-75 0.8458958243244794 1101 76-77 0.6248850800266332 1101 78-79 0.6597490926477292 1101 80-81 0.6620703199797155 1101 82-83 0.7319845178610684 1101 84-85 0.7600989337865371 1101 86-87 0.9634593645571847 1101 88-89 0.9545973381587274 1101 90-91 0.9487713929745105 1101 92-93 0.7195005839965738 1101 94-95 0.7503610590400989 1101 96-97 0.7341689565232592 1101 98-99 0.8223615363294172 1101 100-101 0.4596884166044042 1102 1 0.22575096702727393 1102 2 0.15751283246537184 1102 3 0.16239213681521392 1102 4 0.1155349241440291 1102 5 0.16983720405215053 1102 6 0.14105205133780174 1102 7 0.21831885592354894 1102 8 0.14870479193493935 1102 9 0.3161398892898575 1102 10-11 0.32244492561260785 1102 12-13 0.2717970340630842 1102 14-15 0.3842114526170519 1102 16-17 0.43245154746476544 1102 18-19 0.3110673192633797 1102 20-21 0.3895157026976861 1102 22-23 0.42982481218938773 1102 24-25 0.35896300198048436 1102 26-27 0.39367392321529593 1102 28-29 0.38412069852790154 1102 30-31 0.41472862871973604 1102 32-33 0.509259995248037 1102 34-35 0.40066883528440655 1102 36-37 0.4423665355193762 1102 38-39 0.5165564685070123 1102 40-41 0.6194493639661758 1102 42-43 0.553686624924353 1102 44-45 0.48324622187318766 1102 46-47 0.5369919463671593 1102 48-49 0.23786029568245937 1102 50-51 0.32862219511079616 1102 52-53 0.4021567191215567 1102 54-55 0.20266488975981645 1102 56-57 0.2847309051956657 1102 58-59 0.33664704470481155 1102 60-61 0.2254801335455383 1102 62-63 0.2608487059964162 1102 64-65 0.3886431386469553 1102 66-67 0.354845838671352 1102 68-69 0.168046016939023 1102 70-71 0.19927594455637632 1102 72-73 0.3451161475394713 1102 74-75 0.40117932743755347 1102 76-77 0.4554371150070686 1102 78-79 0.45688237634095685 1102 80-81 0.47380888559179724 1102 82-83 0.5414072931656442 1102 84-85 0.46284603855357176 1102 86-87 0.6331734850559094 1102 88-89 0.5958142073464785 1102 90-91 0.7300826839870069 1102 92-93 0.7246708126051189 1102 94-95 0.7819531599257026 1102 96-97 0.6978288320005213 1102 98-99 0.610474842519114 1102 100-101 0.4946367697673786 1103 1 0.1855298190326451 1103 2 0.14034841057715397 1103 3 0.14709893774757177 1103 4 0.04316043462539909 1103 5 0.09326901675380839 1103 6 0.11751077458895764 1103 7 0.214921260305168 1103 8 0.17148556147484584 1103 9 0.21202120882918507 1103 10-11 0.2388610880174511 1103 12-13 0.21926634776944098 1103 14-15 0.2663762763837667 1103 16-17 0.2630123019246611 1103 18-19 0.2690451814994361 1103 20-21 0.18671575781245053 1103 22-23 0.17559915550078387 1103 24-25 0.1376027772347257 1103 26-27 0.17061241284108775 1103 28-29 0.23404682943311883 1103 30-31 0.08949429528306752 1103 32-33 0.1706496145351437 1103 34-35 0.35103320511978353 1103 36-37 0.3525355924062339 1103 38-39 0.3107188963124159 1103 40-41 0.3880528936075862 1103 42-43 0.3521066683272309 1103 44-45 0.4767269872029445 1103 46-47 0.46466970105287686 1103 48-49 0.42433363965600535 1103 50-51 0.41353625052409626 1103 52-53 0.4863948531805633 1103 54-55 0.5553984290080294 1103 56-57 0.6248083337856869 1103 58-59 0.5381554286556991 1103 60-61 0.4482718112160171 1103 62-63 0.47895027026061143 1103 64-65 0.6001697793637533 1103 66-67 0.5788081309862889 1103 68-69 0.5614988421448359 1103 70-71 0.5461642567815161 1103 72-73 0.5401018521472949 1103 74-75 0.40435206246949207 1103 76-77 0.3673491763375516 1103 78-79 0.49352565750663047 1103 80-81 0.5940004094002269 1103 82-83 0.6872229725508099 1103 84-85 0.601534307146192 1103 86-87 0.8268869571712827 1103 88-89 0.8711807779450496 1103 90-91 0.9369280143502685 1103 92-93 0.7065346102420733 1103 94-95 0.6746604048381108 1103 96-97 0.6180142319461694 1103 98-99 0.7456805468972938 1103 100-101 0.5054448134611356 1104 1 0.02351499899030074 1104 2 0.07339100349646799 1104 3 0.10516615722534084 1104 4 0.0376709174529708 1104 5 0.13192021647152075 1104 6 0.09319701018058169 1104 7 0.10381725536515773 1104 8 0.027591110169268518 1104 9 0.10084222153208344 1104 10-11 0.03491679549804161 1104 12-13 0.09642043651326304 1104 14-15 0.02865996698596973 1104 16-17 0.19091878716343302 1104 18-19 0.18080307670056328 1104 20-21 0.14351709867342066 1104 22-23 0.22550616267556478 1104 24-25 0.2943355680486448 1104 26-27 0.20118646894552938 1104 28-29 0.1498111348906619 1104 30-31 0.12056547706382048 1104 32-33 0.09147890470216424 1104 34-35 0.21014484796852173 1104 36-37 0.19268882840953694 1104 38-39 0.20844296123831185 1104 40-41 0.34705959791245533 1104 42-43 0.3119458973321727 1104 44-45 0.24407180312860532 1104 46-47 -0.014508727552161815 1104 48-49 0.10989347116623804 1104 50-51 0.1748828454429372 1104 52-53 0.1418207823736921 1104 54-55 0.12900610955379221 1104 56-57 0.453044888419889 1104 58-59 0.40523413072579473 1104 60-61 0.5245145335166512 1104 62-63 0.257662632057837 1104 64-65 0.08888254547761676 1104 66-67 0.25794925219306464 1104 68-69 0.1751924951088526 1104 70-71 0.2503693335281625 1104 72-73 0.09090791248603836 1104 74-75 0.1911086712086103 1104 76-77 0.19521964064007236 1104 78-79 0.24207397701968603 1104 80-81 0.2121935551306393 1104 82-83 0.18939764468677822 1104 84-85 0.03881652750610698 1104 86-87 0.14882481002943493 1104 88-89 0.20285531040799754 1104 90-91 0.31054947841754554 1104 92-93 0.5396464883295842 1104 94-95 0.31151460090962146 1104 96-97 0.3314243119273499 1104 98-99 0.3734774990850127 1104 100-101 0.1054946179189109 1105 1 0.08900739744730402 1105 2 0.08277486723596894 1105 3 0.0913656693651177 1105 4 0.06740876349336133 1105 5 0.06846860539823041 1105 6 0.08523559935689207 1105 7 0.05820019727575243 1105 8 0.12920253801134152 1105 9 0.1829069764674074 1105 10-11 0.09223718068070497 1105 12-13 0.10483372077693076 1105 14-15 0.09023760625305499 1105 16-17 0.09000209922378843 1105 18-19 0.11086001736314444 1105 20-21 0.05946615698974256 1105 22-23 0.1232487454983513 1105 24-25 0.11339774895402144 1105 26-27 0.13155257684206134 1105 28-29 0.13957847945612656 1105 30-31 0.09262005642619897 1105 32-33 0.09870568879565411 1105 34-35 0.27841000141758343 1105 36-37 0.0699478410598644 1105 38-39 0.2331025936413127 1105 40-41 0.0969376328568643 1105 42-43 0.3614108820156261 1105 44-45 0.2554312171290647 1105 46-47 0.4007255588104428 1105 48-49 0.4078712976354879 1105 50-51 0.43999020305582803 1105 52-53 0.31655912478350245 1105 54-55 0.35960941111947875 1105 56-57 0.33568490004911666 1105 58-59 0.40861901556809954 1105 60-61 0.5166488510796157 1105 62-63 0.5337390445833705 1105 64-65 0.3672856896232872 1105 66-67 0.35742575973787893 1105 68-69 0.36733088999199026 1105 70-71 0.46296488464003716 1105 72-73 0.5016617558034895 1105 74-75 0.2841766950910696 1105 76-77 0.2704126928864987 1105 78-79 0.49944701281501125 1105 80-81 0.4343809133108074 1105 82-83 0.28292268297813195 1105 84-85 0.32433477262750543 1105 86-87 0.4675191457453387 1105 88-89 0.5344375233646588 1105 90-91 0.5035919747302309 1105 92-93 0.49482450767380826 1105 94-95 0.2735724775830981 1105 96-97 0.5277866332343599 1105 98-99 0.5381787201537662 1105 100-101 -0.0754325646883025 1106 1 -7.800215344175854E-4 1106 2 -0.02148701943767861 1106 3 -0.011395283074499218 1106 4 -0.029364878102981606 1106 5 -0.05100076336071169 1106 6 0.014167082017102928 1106 7 0.0127639134054931 1106 8 0.06469316487211074 1106 9 0.052885211894384554 1106 10-11 0.11392441418445998 1106 12-13 0.13842011860321435 1106 14-15 0.05894299058940078 1106 16-17 -0.005094369850674241 1106 18-19 -0.02037402708312186 1106 20-21 -0.04070340819789209 1106 22-23 -0.01360541817397376 1106 24-25 0.04959572443808469 1106 26-27 0.061371581831487276 1106 28-29 0.05116281033238579 1106 30-31 0.04135079854338386 1106 32-33 0.1368996056659384 1106 34-35 0.06011988924105793 1106 36-37 0.12981444749751603 1106 38-39 0.20877760339430296 1106 40-41 0.16667632109646036 1106 42-43 0.03797854725186056 1106 44-45 0.10768142074416431 1106 46-47 0.2502043725727745 1106 48-49 0.21631037394153196 1106 50-51 0.20484726182701962 1106 52-53 0.26554635349631184 1106 54-55 0.21266107118148625 1106 56-57 0.346166458337791 1106 58-59 0.2633611943524343 1106 60-61 0.2865937210054952 1106 62-63 0.31314072415135996 1106 64-65 0.28490267420329474 1106 66-67 0.2927569166139037 1106 68-69 -0.008645264796555807 1106 70-71 0.06198462332827148 1106 72-73 -0.08119436531156765 1106 74-75 0.1668031931165146 1106 76-77 0.04615956679570132 1106 78-79 0.255486852934375 1106 80-81 0.27388344224468497 1106 82-83 0.3347459792843175 1106 84-85 0.3458988104348961 1106 86-87 0.2535552557727847 1106 88-89 0.4040268368224176 1106 90-91 0.414713124009193 1106 92-93 0.38312947177594836 1106 94-95 0.3071321830084983 1106 96-97 0.4878974066980071 1106 98-99 0.4857841038056989 1106 100-101 0.21304093797067125 1107 1 -0.08688724549522675 1107 2 -0.011118163098537082 1107 3 -0.011928964673444398 1107 4 -0.03593830503918127 1107 5 0.04943026720893329 1107 6 0.06840709477877027 1107 7 0.006211561380553121 1107 8 0.013435089938802491 1107 9 0.13121546937144046 1107 10-11 0.1211022047296737 1107 12-13 0.0735578642231971 1107 14-15 0.14088273190621692 1107 16-17 0.19480051497655637 1107 18-19 0.18130460581008379 1107 20-21 0.1469918589606536 1107 22-23 0.1021034640256957 1107 24-25 0.06642485165144052 1107 26-27 0.1090250363660985 1107 28-29 0.1090414875801784 1107 30-31 0.11955687615871824 1107 32-33 0.150083908034496 1107 34-35 0.06264271823339129 1107 36-37 0.1075674469437331 1107 38-39 0.17362011220361495 1107 40-41 0.28177548990196044 1107 42-43 0.1795398609775276 1107 44-45 0.07056120685651734 1107 46-47 0.15034755227757302 1107 48-49 0.22567363278424324 1107 50-51 -0.005969290473125 1107 52-53 -0.00266998922194972 1107 54-55 -0.037845537262221285 1107 56-57 -0.03842894875815972 1107 58-59 -0.009672873079502153 1107 60-61 0.025451415423169976 1107 62-63 -0.08502854694203421 1107 64-65 0.037315913657089084 1107 66-67 0.14381241301561687 1107 68-69 0.17049212935875602 1107 70-71 0.25379857660551863 1107 72-73 0.12346296311596205 1107 74-75 0.1772477570214832 1107 76-77 0.211989289294241 1107 78-79 0.3791195775378142 1107 80-81 0.317342340980062 1107 82-83 0.17798592590318307 1107 84-85 0.47834263120606835 1107 86-87 0.4812210375093322 1107 88-89 0.4817817492221188 1107 90-91 0.4944489159524821 1107 92-93 0.44171545546850766 1107 94-95 0.4367064114397827 1107 96-97 0.33703686943691835 1107 98-99 0.43330676419874337 1107 100-101 0.16554894967924128 1108 1 -0.0034976225345104694 1108 2 0.0055809553129435585 1108 3 -0.03187439368716127 1108 4 0.0014573215976056986 1108 5 0.010987033800532231 1108 6 0.04668312010724662 1108 7 0.02642780030802072 1108 8 0.10570411758867948 1108 9 0.021251461682176398 1108 10-11 0.005808137387866452 1108 12-13 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-0.6671711141753285 2316 84-85 -0.8078471666626257 2316 86-87 -0.8631185389602862 2316 88-89 -1.1491126309248614 2316 90-91 -1.3535094213520402 2316 92-93 -1.209721255821428 2316 94-95 -0.9185927413448987 2316 96-97 -1.164248764995726 2316 98-99 -1.1823750908653565 2316 100-101 -0.6152474172636495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 11.0 5 23.0 6 67.0 7 169.0 8 367.0 9 720.0 10 1282.0 11 2061.0 12 3210.0 13 4704.0 14 5933.0 15 8152.0 16 11401.0 17 16913.0 18 16654.0 19 9858.0 20 8209.0 21 8293.0 22 8624.0 23 9253.0 24 10725.0 25 12513.0 26 14907.0 27 18077.0 28 22324.0 29 27302.0 30 33876.0 31 41651.0 32 52562.0 33 68567.0 34 96022.0 35 141776.0 36 233382.0 37 422854.0 38 442500.0 39 50604.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.9752105293285 20.429667115521735 16.089648009078616 21.505474346071146 2 15.292915474743223 21.618560498018617 39.64016604312801 23.44835798411015 3 18.65359958572295 26.191004981847687 30.790754089462798 24.364641342966568 4 12.97656399278892 18.232787148549892 38.046085552641706 30.74456330601948 5 13.464336450565062 38.09000556615556 33.824281176701895 14.62137680657748 6 29.76536300472708 37.361586880488275 18.026257854233187 14.846792260551464 7 27.063312942557825 34.25988131073271 21.16562497403845 17.511180772671008 8 26.168851128877268 34.46031829548255 20.243581613409727 19.12724896223045 9 26.631534348164415 17.232152994508613 21.023175699438674 35.113136957888294 10-11 24.54425370592422 27.524445392137043 26.98394676429131 20.947354137647427 12-13 25.977663377742577 24.969663067588634 27.770270083160025 21.282403471508758 14-15 23.04596276731998 25.963321171673254 26.855216244470135 24.135499816536626 16-17 21.42668632867399 29.34011811050879 27.297872487728554 21.935323073088668 18-19 21.38711996428797 27.58336222566592 30.263647611757943 20.765870198288173 20-21 22.85424703207601 26.35624503269078 30.003433847210413 20.786074088022797 22-23 22.23125299423169 26.520903544893397 29.86023688007289 21.387606580802025 24-25 21.920959089383487 26.73545841534915 30.02723275950876 21.316349735758603 26-27 21.654337890438942 26.78786640170321 30.150864122042698 21.40693158581515 28-29 21.40480350917031 27.085743718690374 30.234360072110793 21.275092700028523 30-31 21.78174527348304 26.753484482180884 30.040847297570483 21.423922946765586 32-33 21.562095865260268 26.806328248100996 29.832461486911228 21.799114399727507 34-35 22.06157351965321 26.87043649057193 29.659667854614664 21.408322135160198 36-37 22.11702962222631 26.50881220789409 29.892947131184776 21.481211038694823 38-39 21.81767266081535 26.849882404037622 29.730547223552545 21.601897711594482 40-41 21.898838232793267 26.809469181013114 29.718180191628164 21.57351239456546 42-43 21.77273238381848 26.964238928558178 29.332653130180802 21.930375557442545 44-45 21.887635060138734 27.04525968544862 29.182211829241005 21.884893425171644 46-47 22.12831948469496 27.250132578616004 28.654925901361512 21.96662203532753 48-49 22.03269109614173 27.166508588162802 28.707739385086157 22.093060930609308 50-51 22.101615931070445 27.48849590997996 28.45872470685582 21.951163452093777 52-53 22.378114836903062 27.56746967132489 28.029129041977193 22.025286449794855 54-55 21.95480060147711 27.444193059751708 28.291411781972858 22.309594556798327 56-57 22.057622171576053 27.694171598206648 28.020304006247386 22.227902223969913 58-59 22.250657518138695 27.23549936069048 28.17495341224013 22.338889708930697 60-61 22.205100282438153 27.350581687439174 28.404753424680294 22.039564605442376 62-63 22.09502556672161 27.332077152124207 28.339929946296593 22.23296733485759 64-65 21.878091224991884 27.30070218231484 28.390336491436784 22.430870101256485 66-67 21.832868496051304 27.493775168301006 28.130312846429785 22.543043489217908 68-69 21.970052996708095 27.284456652891087 28.27845301786579 22.46703733253503 70-71 21.912049733198305 27.337529828094357 28.160518001533603 22.58990243717373 72-73 21.976760608234034 27.266879159039743 28.061537865088575 22.694822367637652 74-75 21.794628244501 27.68281774855931 27.808430094901563 22.714123912038126 76-77 21.842984468521013 27.24708545394093 28.107281050330073 22.80264902720798 78-79 21.87393730206818 27.345661644732395 28.118668812647257 22.661732240552166 80-81 21.72511397897464 27.556949331939062 27.99035052325232 22.727586165833973 82-83 21.784025935652135 27.430075841345452 27.954820302229994 22.83107792077242 84-85 21.69039436627519 27.33807610402342 27.876691654366663 23.094837875334733 86-87 21.66596850016925 27.345375065115228 27.891303377508653 23.097353057206867 88-89 21.732627220563845 27.121737715904082 27.84174472327155 23.303890340260523 90-91 21.592036093817903 27.16347844800142 28.015387750800958 23.22909770737972 92-93 21.659517433697673 27.1023868007344 27.94788859935034 23.290207166217588 94-95 21.394003505847234 27.048303707170373 27.94689167596667 23.610801111015725 96-97 21.34952964600912 26.912472896145506 28.050820938714132 23.687176519131235 98-99 21.222455672779304 26.879574682439394 27.775493119300986 24.12247652548031 100-101 21.38411700426631 26.418286818189117 28.32724374578035 23.870352431764218 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 377.0 1 267.0 2 192.5 3 251.5 4 240.5 5 255.5 6 445.5 7 942.5 8 1677.5 9 2178.0 10 2083.5 11 1890.5 12 2010.5 13 2257.0 14 2516.0 15 2637.0 16 2696.0 17 2785.5 18 2957.5 19 3210.0 20 3532.0 21 3937.0 22 4504.0 23 5482.5 24 6610.0 25 8344.5 26 10826.0 27 13431.0 28 15617.0 29 18366.0 30 22368.5 31 26501.0 32 30785.5 33 34748.0 34 38805.0 35 42340.5 36 45540.0 37 49587.0 38 52055.0 39 53016.0 40 54548.5 41 57674.5 42 60463.5 43 62641.5 44 65489.0 45 67399.0 46 67534.5 47 69563.5 48 78298.0 49 78243.5 50 69611.5 51 64542.5 52 60359.0 53 58052.0 54 55276.5 55 50142.0 56 44273.5 57 39739.0 58 36250.0 59 32017.0 60 26833.0 61 21794.0 62 17464.0 63 13019.0 64 9186.0 65 6845.0 66 5040.5 67 3872.0 68 2950.0 69 2038.5 70 1382.0 71 1074.5 72 944.0 73 848.5 74 665.0 75 525.5 76 378.5 77 212.5 78 115.5 79 66.0 80 44.0 81 29.0 82 14.5 83 6.0 84 2.5 85 1.5 86 0.5 87 0.5 88 0.5 89 1.0 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34128010500926304 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.69231067455713E-4 16-17 0.0023538468781067316 18-19 5.5384632426040746E-5 20-21 0.0 22-23 0.0 24-25 4.984616918343667E-4 26-27 0.0012738465457989372 28-29 0.0 30-31 0.002769231621302037 32-33 0.0 34-35 0.01113231111763419 36-37 0.030960009526156778 38-39 0.024009238156688664 40-41 0.015950774138699733 42-43 0.011741542074320639 44-45 0.003489231842840567 46-47 0.0012461542295859169 48-49 9.69231067455713E-4 50-51 8.307694863906112E-5 52-53 1.6615389727812224E-4 54-55 0.0 56-57 0.0 58-59 0.0050676938669827285 60-61 0.011298465014912312 62-63 0.004596924491361382 64-65 0.0022707699294676705 66-67 0.004680001440000443 68-69 0.0036276934239056692 70-71 2.7692316213020373E-5 72-73 0.0 74-75 0.0 76-77 0.22087391411505047 78-79 4.984616918343667E-4 80-81 0.0037938473211837913 82-83 5.261540080473871E-4 84-85 0.0 86-87 0.11196003444924137 88-89 0.41247704999293844 90-91 0.40106781571317407 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0018553851862723651 100-101 7.753848539645706E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1805555.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.159375080822684 #Duplication Level Percentage of deduplicated Percentage of total 1 71.34836748975256 35.78789526322875 2 15.131711823497696 15.179944179394807 3 5.618590005955839 8.45474890602302 4 2.6945392231991354 5.406256142657362 5 1.4144945321178704 3.547508089313652 6 0.8881526070034654 2.6729507846218397 7 0.5621763679378272 1.9738890710677643 8 0.38493713629536397 1.5446564961581544 9 0.28574564136550346 1.289954052266628 >10 1.5108795876282857 13.693652415938157 >50 0.1054640671873774 3.583452237038118 >100 0.05151511064659396 4.53541485005611 >500 0.0024316439701227923 0.7779451905402146 >1k 7.737048995845248E-4 0.7357547479802728 >5k 2.2105854273843562E-4 0.8159775737151425 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8224 0.4554832170717591 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6494 0.35966780297470863 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3856 0.2135631426348131 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2354 0.1303754247308999 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.5384632426040746E-5 0.0 0.0 0.0 0.0 3 5.5384632426040746E-5 0.0 0.0 0.0 0.0 4 5.5384632426040746E-5 0.0 0.0 5.5384632426040746E-5 0.0 5 5.5384632426040746E-5 0.0 0.0 5.5384632426040746E-5 0.0 6 1.1076926485208149E-4 0.0 0.0 5.5384632426040746E-5 0.0 7 1.6615389727812224E-4 0.0 0.0 5.5384632426040746E-5 0.0 8 1.6615389727812224E-4 0.0 0.0 5.5384632426040746E-5 0.0 9 1.6615389727812224E-4 0.0 0.0 5.5384632426040746E-5 0.0 10-11 1.6615389727812224E-4 0.0 0.0 1.1076926485208149E-4 0.0 12-13 2.2153852970416298E-4 0.0 0.0 1.1076926485208149E-4 0.0 14-15 2.2153852970416298E-4 0.0 0.0 1.3846158106510187E-4 0.0 16-17 2.7692316213020373E-4 0.0 0.0 1.6615389727812224E-4 0.0 18-19 3.0461547834322413E-4 0.0 0.0 1.6615389727812224E-4 0.0 20-21 3.323077945562445E-4 0.0 0.0 1.6615389727812224E-4 0.0 22-23 3.323077945562445E-4 0.0 0.0 6.369232728994686E-4 0.0 24-25 4.4307705940832597E-4 0.0 0.0 0.0010246156998817539 0.0 26-27 4.4307705940832597E-4 0.0 0.0 0.0014676927592900799 0.0 28-29 5.261540080473871E-4 0.0 0.0 0.003960001218461913 0.0 30-31 6.092309566864483E-4 0.0 0.0 0.010993849536569088 0.0 32-33 6.092309566864483E-4 0.0 0.0 0.021350775800238708 0.0 34-35 6.369232728994686E-4 0.0 0.0 0.037412319203790526 0.0 36-37 6.64615589112489E-4 0.0 0.0 0.06264001927385209 0.0 38-39 6.64615589112489E-4 0.0 0.0 0.10129849270722852 0.0 40-41 6.923079053255093E-4 0.0 0.0 0.146769275929008 0.0 42-43 9.138464350296723E-4 0.0 0.0 0.20021544622013732 0.0 44-45 0.0011630772809468556 0.0 0.0 0.266455466601682 0.0 46-47 0.0011630772809468556 0.0 0.0 0.34607087571411566 0.0 48-49 0.0012738465457989372 0.0 0.0 0.4353509031848933 0.0 50-51 0.0012738465457989372 0.0 0.0 0.5357909340895182 0.0 52-53 0.0012738465457989372 0.0 0.0 0.6364248112076343 0.0 54-55 0.0012738465457989372 0.0 0.0 0.7483294610244495 0.0 56-57 0.0012738465457989372 0.0 0.0 0.8891725812838711 0.0 58-59 0.0012738465457989372 0.0 0.0 1.0462157065279096 0.0 60-61 0.0012738465457989372 0.0 0.0 1.2245542229397608 0.0 62-63 0.0012738465457989372 0.0 0.0 1.409815818404867 0.0 64-65 0.0012738465457989372 0.0 0.0 1.6095051106169571 0.0 66-67 0.0012738465457989372 0.0 0.0 1.8355021032314163 0.0 68-69 0.0013846158106510186 0.0 0.0 2.1018744928844595 0.0 70-71 0.0013846158106510186 0.0 0.0 2.3950253523154927 0.0 72-73 0.0013846158106510186 0.0 0.0 2.702188523750315 0.0 74-75 0.0013846158106510186 0.0 0.0 3.0275178546208785 0.0 76-77 0.0013846158106510186 0.0 0.0 3.3756102694185444 0.0 78-79 0.0013846158106510186 0.0 0.0 3.773105776340239 0.0 80-81 0.0015784620241421612 0.0 0.0 4.196493598921108 0.0 82-83 0.0016061543403551816 0.0 0.0 4.653915278127778 0.0 84-85 0.0016061543403551816 0.0 0.0 5.120004652309124 0.0 86-87 0.0016061543403551816 2.7692316213020373E-5 0.0 5.617192497597692 0.0 88-89 0.0016338466565682021 5.5384632426040746E-5 0.0 6.16802035939088 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10265 0.0 27.601807 1 CCGTCGA 35 0.008429089 27.119593 30-31 GTATCAA 13795 0.0 25.267885 1 GAGTACT 7420 0.0 23.084915 12-13 GTACTTT 7970 0.0 21.343018 14-15 GTACATG 11130 0.0 21.049871 1 ATCAACG 16620 0.0 20.951052 3 TCAACGC 16765 0.0 20.82644 4 CAACGCA 16765 0.0 20.769848 5 TATCAAC 16915 0.0 20.753935 2 TACATGG 11000 0.0 20.74403 2 AACGCAG 17190 0.0 20.339132 6 AGTACTT 7860 0.0 19.800507 12-13 ACATGGG 11410 0.0 19.333395 3 CATGGGG 4230 0.0 18.9534 4 ACTTTTT 9270 0.0 18.475334 16-17 CGCAGAG 18970 0.0 18.305624 8 ACGCAGA 19000 0.0 18.30169 7 GCAGAGT 19725 0.0 17.269682 9 AGAGTAC 16060 0.0 17.2541 10-11 >>END_MODULE