FastQCFastQC Report
Fri 27 May 2016
SRR939132_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939132_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778242
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81480.4582053511276868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63310.3560257827674748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35930.20205348878274162No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23790.13378381570112505No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA139050.027.322281
TACGCTT950.001247927524.9269375
GGTATCA99350.024.8083271
GAGTACT70750.023.08477612-13
ATCAACG166650.022.6516553
TCAACGC168000.022.4696334
CAACGCA168400.022.4155
TATCAAC168850.022.4119912
AACGCAG171300.022.107276
AGTACTT73550.021.43357312-13
GTACTTT77100.021.12299214-15
GTACATG112800.021.050331
GTGTAAG7900.021.0396691
TACATGG113050.020.3191682
ACGCAGA188900.019.9472777
CGCAGAG189400.019.7946458
GCAGAGT195150.018.8232969
ACATGGG119200.018.7150763
AGAGTAC157800.018.3141710-11
ACTTTTT90550.017.95956216-17
Produced by FastQC (version 0.11.5)