FastQCFastQC Report
Fri 27 May 2016
SRR939132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778242
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78030.43880416726182375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63810.35883754854513616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37200.20919537385800133No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23430.13175934434120892No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA135200.028.4382171
GGTATCA95450.027.8524741
ATCAACG163550.023.4135063
TCAACGC166200.023.0401844
TATCAAC166550.022.9632782
CAACGCA167650.022.8692155
GAGTACT73100.022.81726812-13
AACGCAG170600.022.4737646
AGTACTT76950.022.10732712-13
GTACTTT78100.021.35649714-15
GTATACG1150.003659451820.7146551
ACGCAGA186950.020.4829047
CGCAGAG187500.020.3722068
GCAGAGT195350.019.3106639
GTACATG106600.019.0396041
GGACCGT1507.7773485E-418.9803166
TACTTTT88650.018.97549814-15
AGAGTAC160800.018.85690910-11
TACATGG106850.018.6072832
ACCGCGA901.2438184E-418.45360612-13
Produced by FastQC (version 0.11.5)