##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939131_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 659728 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.087846809594254 33.0 31.0 34.0 30.0 34.0 2 32.1142410205418 34.0 31.0 34.0 30.0 34.0 3 32.20297607498848 34.0 31.0 34.0 30.0 34.0 4 35.55785717750346 37.0 35.0 37.0 33.0 37.0 5 35.6568237212912 37.0 35.0 37.0 35.0 37.0 6 35.838157543714985 37.0 36.0 37.0 35.0 37.0 7 35.80532886280407 37.0 36.0 37.0 35.0 37.0 8 35.82842322896709 37.0 36.0 37.0 35.0 37.0 9 37.41822235830524 39.0 37.0 39.0 35.0 39.0 10-11 37.34357568573715 39.0 37.0 39.0 34.5 39.0 12-13 37.34023415710717 39.0 37.0 39.0 35.0 39.0 14-15 38.401262338418256 40.0 38.0 41.0 34.0 41.0 16-17 38.46854233866078 40.0 38.0 41.0 34.0 41.0 18-19 38.53575412897436 40.0 38.0 41.0 34.0 41.0 20-21 38.41221154778939 40.0 38.0 41.0 34.0 41.0 22-23 38.362493330584726 40.0 38.0 41.0 34.0 41.0 24-25 38.33507748647928 40.0 38.0 41.0 34.0 41.0 26-27 38.144199579220526 40.0 38.0 41.0 34.0 41.0 28-29 38.11823509082531 40.0 38.0 41.0 34.0 41.0 30-31 38.10991120583029 40.0 38.0 41.0 34.0 41.0 32-33 37.85409517255596 40.0 37.0 41.0 33.5 41.0 34-35 37.43041374627119 40.0 37.0 41.0 33.0 41.0 36-37 37.07022136395605 39.5 36.0 41.0 32.0 41.0 38-39 36.80539934639731 39.0 35.5 41.0 31.0 41.0 40-41 36.92029821380933 39.5 36.0 41.0 31.0 41.0 42-43 36.74440905949119 40.0 36.0 41.0 31.0 41.0 44-45 36.49871992700022 39.0 35.5 41.0 30.0 41.0 46-47 36.09694374045061 39.0 35.0 41.0 29.5 41.0 48-49 35.9678352290641 39.0 35.5 41.0 28.5 41.0 50-51 34.83649549511314 38.0 34.0 40.0 26.5 40.5 52-53 35.17367915262047 38.5 35.0 39.5 28.0 40.5 54-55 35.83733447723911 39.0 35.5 41.0 28.0 41.0 56-57 35.628382454587346 39.0 35.0 41.0 27.5 41.0 58-59 35.48731219532898 39.0 35.0 41.0 27.0 41.0 60-61 34.90822581427497 39.0 35.0 40.0 26.0 41.0 62-63 34.70065466374021 38.0 35.0 40.0 26.0 41.0 64-65 34.29214994664468 37.5 34.5 39.5 25.0 41.0 66-67 33.667718362719185 37.0 34.0 39.0 22.0 41.0 68-69 33.12569801493949 36.5 33.0 39.0 21.5 40.0 70-71 32.380742518128685 35.5 32.5 38.0 19.5 39.5 72-73 31.808979609778575 35.0 31.5 37.0 17.0 39.0 74-75 31.392689108238546 35.0 31.0 37.0 13.5 39.0 76-77 30.96927370067664 35.0 31.0 36.5 7.5 38.5 78-79 30.448543945383552 34.5 31.0 36.0 4.5 37.0 80-81 29.87138335798996 34.0 30.0 35.5 2.0 37.0 82-83 29.333110766861495 34.0 29.5 35.0 2.0 36.0 84-85 28.903063080542285 34.0 29.0 35.0 2.0 36.0 86-87 28.789078984066162 34.0 29.0 35.0 2.0 36.0 88-89 28.228267558751483 34.0 29.0 35.0 2.0 35.0 90-91 27.70114350156428 34.0 27.0 35.0 2.0 35.0 92-93 27.17503577231829 34.0 25.5 35.0 2.0 35.0 94-95 26.62026319937914 33.0 25.0 35.0 2.0 35.0 96-97 25.91538558314942 33.0 23.5 35.0 2.0 35.0 98-99 25.292688047195206 33.0 19.0 35.0 2.0 35.0 100-101 22.751211863070843 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30826095099487105 1101 2 0.419197001689092 1101 3 0.38484928023179066 1101 4 0.1897301658837236 1101 5 0.4024126199780085 1101 6 0.33858227985446376 1101 7 0.31231603613714753 1101 8 0.2534369768925515 1101 9 0.5941114254059983 1101 10-11 0.5226695135506034 1101 12-13 0.47220421878314056 1101 14-15 0.4537317296982195 1101 16-17 0.6597667691346558 1101 18-19 0.7984480056465841 1101 20-21 0.873218935385168 1101 22-23 0.8486345502820498 1101 24-25 0.7956069790583982 1101 26-27 0.7738806676051766 1101 28-29 0.7338265851533095 1101 30-31 0.8433769525518215 1101 32-33 0.676828958291047 1101 34-35 0.4741050910212863 1101 36-37 0.580760825721967 1101 38-39 0.7481844918509708 1101 40-41 0.7549127042238339 1101 42-43 0.7074959466931503 1101 44-45 0.4649797070636055 1101 46-47 0.16193328155127062 1101 48-49 0.3056588543614893 1101 50-51 0.12909185594279649 1101 52-53 0.29569347891752074 1101 54-55 0.31224265071955415 1101 56-57 0.29165917351413384 1101 58-59 0.09269326879355333 1101 60-61 0.2545357265619259 1101 62-63 0.4488542420509489 1101 64-65 0.5032889166242853 1101 66-67 0.43442422868178454 1101 68-69 0.47384784787433887 1101 70-71 0.043851547755735965 1101 72-73 -0.2164286716333912 1101 74-75 -0.0773292981922431 1101 76-77 -0.08978362434320175 1101 78-79 -0.051929761280877784 1101 80-81 -0.20310383979142088 1101 82-83 -0.11103782646969407 1101 84-85 -0.341123544912481 1101 86-87 -0.19307291548129513 1101 88-89 0.21508219728543665 1101 90-91 0.16828618069250112 1101 92-93 0.15591367025376002 1101 94-95 -0.029926155026036128 1101 96-97 -0.17180984247190878 1101 98-99 0.10602195360509015 1101 100-101 -0.25847097073358327 1102 1 0.22445160127134045 1102 2 0.42898281063406785 1102 3 0.44328015612371274 1102 4 0.41034825820587884 1102 5 0.3516883134386859 1102 6 0.3670551322317124 1102 7 0.30299046703915167 1102 8 0.2315870085544418 1102 9 0.6301276415163528 1102 10-11 0.4416199708527557 1102 12-13 0.3732118502779045 1102 14-15 0.4051568411676598 1102 16-17 0.5060025947266027 1102 18-19 0.6018701010761731 1102 20-21 0.5982125703660159 1102 22-23 0.5701208964999225 1102 24-25 0.81782089592798 1102 26-27 0.7441315472834731 1102 28-29 0.7493392792863887 1102 30-31 0.7792599928766677 1102 32-33 0.7912768989625434 1102 34-35 0.7124195686653749 1102 36-37 0.6047161465515813 1102 38-39 0.6360459106953442 1102 40-41 0.5784711089868253 1102 42-43 0.47641147799271977 1102 44-45 0.5010122437359072 1102 46-47 0.5123700197890386 1102 48-49 0.42859022313437123 1102 50-51 0.37626023844453726 1102 52-53 0.43815729623167243 1102 54-55 0.30817883079410535 1102 56-57 0.178791063239359 1102 58-59 0.4100237471165826 1102 60-61 0.4200670261356265 1102 62-63 0.5837681674072286 1102 64-65 0.677618631145009 1102 66-67 0.6181855959531859 1102 68-69 0.2541616596395002 1102 70-71 0.15844310937238504 1102 72-73 0.22095912450320654 1102 74-75 0.2188546280127035 1102 76-77 -0.1745917924580489 1102 78-79 -0.023068479529225527 1102 80-81 0.09511199637625012 1102 82-83 -0.21366315206490682 1102 84-85 -0.2647531073255749 1102 86-87 -0.43717629644891076 1102 88-89 -0.35275585051422453 1102 90-91 -0.388573025083673 1102 92-93 -0.597622995520716 1102 94-95 -0.16392449685693933 1102 96-97 -0.46130723444610666 1102 98-99 -0.6295490375752806 1102 100-101 -0.9120164496115102 1103 1 0.3049274675046263 1103 2 0.2551259045354257 1103 3 0.32139082328713897 1103 4 0.20574761840732236 1103 5 0.1214314947020938 1103 6 0.2655674078846815 1103 7 0.26516783769834973 1103 8 0.3585189303910994 1103 9 0.5470951505416224 1103 10-11 0.45841147103904234 1103 12-13 0.4569445224004127 1103 14-15 0.6081901029840182 1103 16-17 0.745766460240425 1103 18-19 0.582900973283472 1103 20-21 0.6389097966208794 1103 22-23 0.5671949101438187 1103 24-25 0.42951906004016394 1103 26-27 0.5384196108008155 1103 28-29 0.3759018395083942 1103 30-31 0.4026524668928815 1103 32-33 0.5089240333587881 1103 34-35 0.477194033251628 1103 36-37 0.275496109808131 1103 38-39 0.31736070956991824 1103 40-41 0.3416781955908803 1103 42-43 0.3157038453758432 1103 44-45 0.31045698081467776 1103 46-47 0.15797879196679787 1103 48-49 0.15163003710459577 1103 50-51 0.5212298544369318 1103 52-53 0.5115735378648267 1103 54-55 0.5510999351532178 1103 56-57 0.8757154051333131 1103 58-59 0.9640629572608574 1103 60-61 0.9108329857692254 1103 62-63 0.5719518075783512 1103 64-65 0.5534810102192296 1103 66-67 0.44818610984760454 1103 68-69 0.5301550817909231 1103 70-71 0.521348925372493 1103 72-73 0.22007586846816096 1103 74-75 0.19450887984666565 1103 76-77 0.48521010124289177 1103 78-79 0.5831278024446434 1103 80-81 0.6356935963865276 1103 82-83 0.6644250322176326 1103 84-85 0.5864262803692206 1103 86-87 0.2951842775711562 1103 88-89 0.6906328977674399 1103 90-91 0.4850121593401866 1103 92-93 0.4394833934716651 1103 94-95 0.253675744673437 1103 96-97 0.337204398838832 1103 98-99 0.16641881832872585 1103 100-101 0.6826718091857167 1104 1 0.5022466863929296 1104 2 0.49535749222984293 1104 3 0.5051315125104239 1104 4 0.39523283858423497 1104 5 0.44497082426595824 1104 6 0.41372693001678584 1104 7 0.2576258827513698 1104 8 0.33637177646296124 1104 9 0.5826281454654776 1104 10-11 0.6872127066551315 1104 12-13 0.6363542327050453 1104 14-15 0.6709930607550874 1104 16-17 0.6013177003726611 1104 18-19 0.6464039328610056 1104 20-21 0.8479499059716957 1104 22-23 0.8287201011765646 1104 24-25 0.7501710703249174 1104 26-27 0.7886378909340124 1104 28-29 0.8820046509565174 1104 30-31 0.9570990590131245 1104 32-33 0.873712286575774 1104 34-35 0.8920346737005929 1104 36-37 0.956069479029722 1104 38-39 1.03034792588101 1104 40-41 1.070566743260251 1104 42-43 1.0386461411409087 1104 44-45 1.0674449558374022 1104 46-47 0.8791823735674384 1104 48-49 1.1092987450668517 1104 50-51 1.095908756834099 1104 52-53 1.117433380943993 1104 54-55 0.9872823511078366 1104 56-57 0.9268546768905708 1104 58-59 0.953845941647053 1104 60-61 1.1162360964869293 1104 62-63 1.1725563682782933 1104 64-65 1.283304399010774 1104 66-67 1.0969357410294478 1104 68-69 0.9851276537631506 1104 70-71 0.6781688999616691 1104 72-73 0.9111312002238527 1104 74-75 0.8122883235785672 1104 76-77 0.8588093449398677 1104 78-79 0.7823979941096546 1104 80-81 0.522005776116881 1104 82-83 0.8272559900609657 1104 84-85 0.7075235821256989 1104 86-87 0.7022496291376541 1104 88-89 0.8899949176264137 1104 90-91 0.9584723059040066 1104 92-93 0.7634367025984865 1104 94-95 0.49641379044904 1104 96-97 0.786736526663681 1104 98-99 0.8568840410346752 1104 100-101 0.4912085193879818 1105 1 0.3134744244984091 1105 2 0.33036566567903947 1105 3 0.4436901175750947 1105 4 0.26469568523886267 1105 5 0.19775696668472165 1105 6 0.23966114092045387 1105 7 0.1917134677122192 1105 8 0.27397824639326984 1105 9 0.42336633698229775 1105 10-11 0.39664658775296147 1105 12-13 0.3889066431555861 1105 14-15 0.6017180600381238 1105 16-17 0.5278039775816481 1105 18-19 0.500221077029174 1105 20-21 0.5459639012110244 1105 22-23 0.6427483210853708 1105 24-25 0.47499828074105466 1105 26-27 0.49286800937733233 1105 28-29 0.4939608297973166 1105 30-31 0.5496999365834583 1105 32-33 0.5987847004677747 1105 34-35 0.6901562143240625 1105 36-37 0.508788226341089 1105 38-39 0.8395122265492247 1105 40-41 0.6384723327523503 1105 42-43 0.4417850324196948 1105 44-45 0.566835531993874 1105 46-47 0.76236084154759 1105 48-49 0.78845071386435 1105 50-51 0.4366469483509192 1105 52-53 0.42403810146938525 1105 54-55 0.5295304798181562 1105 56-57 0.6169633694254912 1105 58-59 0.6471680902604362 1105 60-61 0.4953163683616566 1105 62-63 0.4487960080729536 1105 64-65 0.5675439814478977 1105 66-67 0.5297471207650304 1105 68-69 0.4524453284885581 1105 70-71 0.4996105816846921 1105 72-73 0.44132564227840376 1105 74-75 0.5950064973145395 1105 76-77 0.5603895451176726 1105 78-79 0.7982372782875764 1105 80-81 0.7533846513355584 1105 82-83 0.7218199293766894 1105 84-85 0.5740310816238257 1105 86-87 0.3228065708337198 1105 88-89 0.3387359196626072 1105 90-91 0.2598116044213157 1105 92-93 0.49999066170256157 1105 94-95 0.2951619621775059 1105 96-97 0.20680449534646428 1105 98-99 0.25308267018951014 1105 100-101 -0.2463950826654191 1106 1 0.38431207232662956 1106 2 0.37751264916587957 1106 3 0.2443005050621494 1106 4 0.33337517730927146 1106 5 0.3831403663250299 1106 6 0.3665617894571582 1106 7 0.48678676870954973 1106 8 0.44758988735463134 1106 9 0.4538475621171827 1106 10-11 0.5062710904506531 1106 12-13 0.5572809414824746 1106 14-15 0.5834364098119522 1106 16-17 0.6584977483593448 1106 18-19 0.5550605778194111 1106 20-21 0.6458985025516952 1106 22-23 0.6244176218647013 1106 24-25 0.7267149955483987 1106 26-27 0.6771123823676817 1106 28-29 0.6100134757180768 1106 30-31 0.6280475304577848 1106 32-33 0.6348466111986042 1106 34-35 0.8106150949170114 1106 36-37 0.8688895942630523 1106 38-39 0.9272690525342924 1106 40-41 1.0561081712176659 1106 42-43 0.8072790647920272 1106 44-45 0.8401883032620674 1106 46-47 0.8344328890706123 1106 48-49 0.6925953538992289 1106 50-51 0.8457455368298596 1106 52-53 0.6298122580991787 1106 54-55 0.7489893059122181 1106 56-57 0.659832190614658 1106 58-59 0.5778618610381372 1106 60-61 0.8784898383055264 1106 62-63 0.6557991145092714 1106 64-65 0.6502787353854416 1106 66-67 0.555745812791848 1106 68-69 0.6109471542011207 1106 70-71 0.7558725033153024 1106 72-73 0.6229377192308228 1106 74-75 0.325286076583172 1106 76-77 0.4023754844059191 1106 78-79 0.11728463781670584 1106 80-81 0.37985203375422216 1106 82-83 0.40832600600211677 1106 84-85 0.40218247952016384 1106 86-87 0.26818779051871644 1106 88-89 0.13521529183548253 1106 90-91 0.3780932546474851 1106 92-93 0.5816490627053419 1106 94-95 0.6513911844784523 1106 96-97 0.6178568687728081 1106 98-99 0.32316550849132497 1106 100-101 -0.13375951669452135 1107 1 0.02471273429853227 1107 2 -0.011087717602336511 1107 3 0.053854369795253376 1107 4 0.03657371421221711 1107 5 -0.0697960157897981 1107 6 0.008987628228233291 1107 7 -0.13843873410581864 1107 8 -0.027643318587209365 1107 9 -0.0015910936623981797 1107 10-11 -0.09979723379630201 1107 12-13 0.01683083040835953 1107 14-15 0.12206883152582293 1107 16-17 0.06792264784596824 1107 18-19 0.06662272217103293 1107 20-21 0.15879626963896953 1107 22-23 -0.018254219977684727 1107 24-25 -0.008393121559002736 1107 26-27 -0.15844574641864995 1107 28-29 -0.132728191238499 1107 30-31 -0.05067991304848363 1107 32-33 -0.017157195636798406 1107 34-35 0.024282429187977073 1107 36-37 -0.014297462496571711 1107 38-39 0.09481702313320284 1107 40-41 0.10595982391102154 1107 42-43 -0.018091058010718086 1107 44-45 -0.18273222532150157 1107 46-47 -0.11342439932946746 1107 48-49 0.002372857788067506 1107 50-51 0.011689517706223285 1107 52-53 0.12713977018811562 1107 54-55 0.20385395452541388 1107 56-57 0.06257152178449132 1107 58-59 0.3280904699528904 1107 60-61 0.09334246010189418 1107 62-63 0.0033790903562405106 1107 64-65 -0.029332082785984426 1107 66-67 -0.05466856571494105 1107 68-69 -0.028281887082663104 1107 70-71 0.03994144381237419 1107 72-73 0.09100080535177568 1107 74-75 0.20640198627270934 1107 76-77 0.15601210556615897 1107 78-79 -0.009456641498662322 1107 80-81 0.11743384841202698 1107 82-83 0.13035868713741294 1107 84-85 0.1790726031180725 1107 86-87 0.16793532676068423 1107 88-89 0.28504482910122064 1107 90-91 -0.03418677139405091 1107 92-93 0.30401968745374575 1107 94-95 -0.07335374264003391 1107 96-97 -0.15382867469518757 1107 98-99 0.03014106224672375 1107 100-101 0.22447438004382647 1108 1 0.1930985954637805 1108 2 0.08742956714235106 1108 3 0.007107319385809774 1108 4 0.08917194589787414 1108 5 0.16605362949133706 1108 6 0.18707665834333653 1108 7 0.23135448255588642 1108 8 0.20756072875983733 1108 9 0.15734359774658913 1108 10-11 0.2943595049021326 1108 12-13 0.18477989297135622 1108 14-15 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2316 70-71 -1.9635580683934428 2316 72-73 -1.6813673897366677 2316 74-75 -1.6846965919990744 2316 76-77 -1.9553054502744018 2316 78-79 -1.7524959829217046 2316 80-81 -1.717298924414731 2316 82-83 -1.941236527729533 2316 84-85 -1.9190169610095076 2316 86-87 -1.9044783592460206 2316 88-89 -2.0793712246255645 2316 90-91 -2.4280495167070235 2316 92-93 -2.4476072066309733 2316 94-95 -2.588293581357373 2316 96-97 -2.683979653389361 2316 98-99 -2.8855317466073807 2316 100-101 -2.399498420406335 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2744.0 3 430.0 4 424.0 5 441.0 6 470.0 7 490.0 8 661.0 9 837.0 10 1070.0 11 1521.0 12 2209.0 13 3665.0 14 3234.0 15 3432.0 16 4382.0 17 5704.0 18 5552.0 19 3742.0 20 3931.0 21 4258.0 22 4525.0 23 5080.0 24 5752.0 25 6642.0 26 7416.0 27 8938.0 28 10144.0 29 12142.0 30 14408.0 31 17478.0 32 22250.0 33 29048.0 34 40193.0 35 63444.0 36 93013.0 37 135672.0 38 117108.0 39 17276.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.34711435076124 20.154604917975444 15.078009632771497 16.420271098491817 2 13.304814165093509 27.849134712855783 32.696988642916544 26.149062479134166 3 29.26477304972072 25.27855713247378 31.171389075613675 14.285280742191828 4 10.58285606757294 16.484258576367075 45.14569884151135 27.78718651454864 5 9.012122509133242 43.87719945299208 35.470519399505505 11.640158638369176 6 23.992778742803395 40.13380564805474 15.713212928477697 20.160202680664174 7 21.921412088137167 36.498515420059505 23.42146746592749 18.15860502587584 8 22.170888428654877 24.50230243443562 21.703270443114317 31.623538693795183 9 18.98101792010292 25.078889718242337 28.916419377742177 27.023672983912565 10-11 24.644090321562725 24.03239900721529 27.036619555916964 24.286891115305025 12-13 32.1745245126411 24.24987426789018 25.020879495159974 18.554721724308738 14-15 23.942332856693127 27.577972395325638 32.81512302248048 15.664571725500757 16-17 17.075677496086115 30.382043471401353 32.06645408318062 20.47582494933191 18-19 15.938042409603456 36.66863400779249 29.436303664413078 17.957019918190976 20-21 20.8732906851826 28.139965039846075 25.68547089992049 25.30127337505083 22-23 17.711226035150972 28.303180947698436 29.371488923892166 24.61410409325843 24-25 24.999734411874066 28.280430708057946 26.86598423165355 19.85385064841444 26-27 22.22868906758292 23.29232914944458 36.44900378120749 18.029978001765016 28-29 16.781711937366406 35.0880614195299 30.100532139705454 18.02969450339824 30-31 18.806928413125107 28.292411264703226 28.58095604453134 24.319704277640323 32-33 19.797766908457206 29.774604482418788 30.578347494050075 19.849281115073936 34-35 24.44745037926483 26.383921239438674 28.896740816438253 20.27188756485824 36-37 21.227910122011213 24.73822647388966 35.341291756209074 18.69257164789005 38-39 19.967978634150946 29.483942876197755 32.53716412206185 18.010914367589447 40-41 18.60773117328539 31.389833140254435 31.054545675971497 18.947890010488674 42-43 17.048952656941566 31.94687563529269 25.65017805326903 25.35399365449671 44-45 20.23375488099807 26.178064292110605 27.982129912218177 25.60605091467315 46-47 24.389950440612502 30.191018389060993 28.65146378387599 16.76756738645052 48-49 21.839699377307678 28.11528947272218 29.900655593872997 20.144355556097146 50-51 14.498772832810559 29.987427533169303 36.38918143417503 19.12461819984511 52-53 19.287621409130303 33.42012394887908 26.704830168103193 20.587424473887424 54-55 16.275035939670087 26.93061511659073 31.078601276772012 25.715747666967182 56-57 22.27826824390626 25.918180759331396 28.235395321113 23.56815567564934 58-59 26.88667828628403 25.553365249225568 27.533013159597218 20.026943304893177 60-61 20.633094663675116 28.18493132446063 34.16163953958135 17.020334472282897 62-63 18.248872064818013 28.814918132386012 32.65537944759727 20.280830355198702 64-65 17.066895956631907 34.18984508457098 29.397298937807637 19.345960020989477 66-67 19.827102946104866 27.731849617646166 26.33785759985376 26.103189836395213 68-69 16.886423140589045 28.666736241193046 29.76923856267154 24.677602055546373 70-71 23.88764561875498 27.9508792348836 26.649736526599465 21.511738619761953 72-73 20.70036261107392 23.375733162119367 34.825362515870424 21.098541710936292 74-75 16.777848700015692 34.31198404706664 28.62303882662458 20.28712842629309 76-77 19.11355062542168 26.349495961754315 28.77891613881879 25.758037274005215 78-79 19.387325493133925 27.159690198684878 31.380592270802833 22.072392037378368 80-81 23.173468875005142 26.522978485601996 28.56295576673879 21.740596872654073 82-83 19.958347928056895 24.669600840656077 33.94156526506556 21.430485966221465 84-85 18.805092076487753 27.805089030623126 31.505281529267716 21.884537363621412 86-87 18.958888421001333 29.40092032551775 30.130571087645663 21.509620165835255 88-89 18.418745844626937 28.523851555794522 26.805573854640198 26.251828744938344 90-91 19.89087887219666 23.697245616988344 29.690681287242366 26.72119422357263 92-93 23.05873141484436 28.07867515954615 29.84668793694776 19.015905488661733 94-95 20.628985524908675 25.819270060538845 30.57211800657488 22.979626407977598 96-97 15.101973664166326 26.733278086173385 34.89292308254779 23.271825167112503 98-99 19.623022038617847 29.309472888905137 27.48056312111186 23.58694195136515 100-101 18.028881850643838 24.192719300182844 30.909124139564387 26.86927470960893 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3195.0 1 1906.5 2 771.5 3 936.0 4 758.0 5 721.5 6 1355.5 7 2396.5 8 3206.0 9 3257.0 10 2509.5 11 1791.5 12 1618.5 13 1559.0 14 1476.5 15 1465.0 16 1543.5 17 1437.5 18 1198.5 19 1150.0 20 1186.0 21 1298.5 22 1414.0 23 1519.5 24 1649.5 25 2029.0 26 2917.0 27 3762.0 28 3880.0 29 4376.0 30 6047.0 31 7600.5 32 9894.5 33 12945.5 34 15103.5 35 16697.5 36 19081.5 37 20554.0 38 19021.5 39 17133.0 40 15677.0 41 13861.5 42 11488.0 43 10799.0 44 11328.5 45 11593.5 46 18645.5 47 52045.5 48 120925.5 49 117532.5 50 46238.5 51 13373.0 52 4902.0 53 2586.5 54 1470.0 55 982.0 56 723.5 57 590.0 58 544.5 59 491.0 60 414.0 61 361.0 62 323.5 63 281.0 64 236.5 65 219.0 66 207.5 67 195.5 68 163.0 69 133.5 70 109.5 71 93.0 72 87.5 73 73.0 74 55.5 75 48.0 76 41.0 77 29.5 78 23.0 79 14.0 80 11.0 81 12.5 82 9.5 83 6.0 84 2.5 85 0.5 86 1.0 87 1.5 88 1.5 89 1.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.5 95 1.0 96 1.5 97 1.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3305907889311959 2 0.11459268061989183 3 0.10716537724638033 4 0.11610845681856766 5 0.13202410690466373 6 0.08533819998544855 7 0.09382654669803313 8 0.0974644095748551 9 0.08791501952319744 10-11 0.08670239856425678 12-13 0.08920342929207188 14-15 0.09102236073048287 16-17 0.08124560424902383 18-19 0.09579705575631169 20-21 0.1048917129483666 22-23 0.11247059394174569 24-25 0.123232604952344 26-27 0.12300523852254261 28-29 0.11891264278611792 30-31 0.11087902893313609 32-33 0.1040580360390949 34-35 0.324376106516625 36-37 0.47731792496301506 38-39 0.45154972958552614 40-41 0.42881308660538886 42-43 0.46117490844711756 44-45 0.46989062158950357 46-47 0.476256881623942 48-49 0.48868624645308373 50-51 0.47512004947493514 52-53 0.4800463221206315 54-55 0.4656464482332113 56-57 0.4435919045424781 58-59 0.423356292290156 60-61 0.3855376761331943 62-63 0.4542781267431426 64-65 0.4863367933451362 66-67 0.4953556617272573 68-69 0.5089218587054058 70-71 0.5009640336623578 72-73 0.4910356995610312 74-75 0.4997514127034172 76-77 0.4745895278053986 78-79 0.45238340649479786 80-81 0.4554907477020833 82-83 0.4695874663497684 84-85 0.46989062158950357 86-87 0.4717853418378483 88-89 0.4848210171464603 90-91 0.5004335119928213 92-93 0.49361251909878007 94-95 0.49869036936434413 96-97 0.4977809036451386 98-99 0.4917177988504353 100-101 0.4784547571120219 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 659728.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.538112676739503 #Duplication Level Percentage of deduplicated Percentage of total 1 75.42434731837517 10.965276598840735 2 8.46192342981066 2.4604079256905877 3 3.6199849862373847 1.5788324885407319 4 2.023730085912086 1.1768486406519052 5 1.4023271332054383 1.0193594936094876 6 1.064517474351489 0.928564499308806 7 0.82679956626908 0.8414073678849465 8 0.7016848778046543 0.8160939053670604 9 0.564058720493786 0.7380314311352557 >10 4.71578113270498 14.355006911939466 >50 0.7121111018433564 7.205848470884971 >100 0.4107932271248645 10.751552154827444 >500 0.02398031528901493 2.4496459146799894 >1k 0.030236049712236215 11.289349550117624 >5k 0.0072983568270915 7.444734799796279 >10k+ 0.010426224038702143 25.97903984672471 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 42740 6.478427473140445 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 27249 4.130338563771736 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 19760 2.9951737685834163 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14876 2.254868673150147 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 11742 1.7798244124851452 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 11685 1.7711844881526932 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 11655 1.766637159556666 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11355 1.7211638735963912 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10304 1.5618557951155627 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 10025 1.5195656391725074 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 9880 1.4975868842917082 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 7276 1.102878762156525 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7271 1.1021208740571873 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6681 1.0126900783353139 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6141 0.9308381636068197 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6068 0.9197729973564862 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5798 0.8788470399922391 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4945 0.7495513302451919 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4907 0.7437913806902239 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4657 0.7058969757233284 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4345 0.6586047583246429 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4106 0.6223777071762909 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3992 0.6050978585113864 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3716 0.563262435427934 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3657 0.5543193558557467 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3588 0.5438605000848835 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3538 0.5362816190915044 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3272 0.4959619722067276 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2910 0.44109087381466305 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2516 0.3813692915868358 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2109 0.31967720030073 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2024 0.3067931026119855 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1972 0.29891106637887127 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1740 0.2637450585695923 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1723 0.26116823903184344 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1691 0.2563177551960808 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1666 0.2525283146993913 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1523 0.23085271505832705 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1521 0.2305495598185919 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1448 0.21948439356825844 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1339 0.202962433002692 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1286 0.1949288191497102 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1093 0.1656743385152669 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1086 0.16461329517619383 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1055 0.1599143889602988 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1054 0.1597628113404312 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 910 0.1379356340794994 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 904 0.13702616836029394 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 893 0.13535881454175056 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 834 0.1264157349695632 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 826 0.12520311401062256 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 802 0.1215652511338006 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 780 0.1182305434967138 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 780 0.1182305434967138 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 747 0.1132284820410836 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 744 0.11277374918148085 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 743 0.11262217156161328 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 718 0.10883273106492372 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 696 0.10549802342783693 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 692 0.1048917129483666 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.578880993379089E-5 0.0 12-13 0.0 0.0 0.0 1.5157761986758179E-4 0.0 14-15 0.0 0.0 0.0 1.5157761986758179E-4 0.0 16-17 0.0 0.0 0.0 1.5157761986758179E-4 0.0 18-19 0.0 0.0 0.0 1.5157761986758179E-4 0.0 20-21 0.0 0.0 0.0 6.063104794703271E-4 0.0 22-23 0.0 0.0 0.0 0.0026526083476826816 0.0 24-25 0.0 0.0 0.0 0.005987315984769481 0.0 26-27 0.0 0.0 0.0 0.011595687919870007 0.0 28-29 0.0 0.0 0.0 0.031376567312589435 0.0 30-31 0.0 0.0 0.0 0.0724541022967041 0.0 32-33 0.0 0.0 0.0 0.15635231489341062 0.0 34-35 0.0 0.0 0.0 0.2762502122086678 0.0 36-37 0.0 0.0 0.0 0.4394235199961196 0.0 38-39 0.0 0.0 0.0 0.6777035384279582 0.0 40-41 0.0 0.0 0.0 0.9272760895399316 0.0 42-43 0.0 0.0 0.0 1.1842001552154828 0.0 44-45 0.0 0.0 0.0 1.4825806999248174 0.0 46-47 0.0 0.0 0.0 1.8823666723255645 0.0 48-49 0.0 0.0 0.0 2.207576455751461 0.0 50-51 0.0 0.0 0.0 2.565451216258822 0.0 52-53 0.0 0.0 0.0 2.9062734945310797 0.0 54-55 0.0 0.0 0.0 3.300451094996726 0.0 56-57 0.0 0.0 0.0 3.8961511410763223 0.0 58-59 0.0 0.0 0.0 4.3863531637280815 0.0 60-61 0.0 0.0 0.0 4.949842965585818 0.0 62-63 0.0 0.0 0.0 5.473392064608445 0.0 64-65 0.0 0.0 0.0 5.959425702713846 0.0 66-67 0.0 0.0 0.0 6.582182353939805 0.0 68-69 0.0 0.0 0.0 7.321199039604201 0.0 70-71 0.0 0.0 0.0 8.06309570004608 0.0 72-73 0.0 0.0 0.0 8.69623541823297 0.0 74-75 0.0 0.0 0.0 9.507403050954332 0.0 76-77 0.0 0.0 0.0 10.158580505905464 0.0 78-79 0.0 0.0 0.0 11.273509688841461 0.0 80-81 0.0 0.0 0.0 12.363883297358912 0.0 82-83 0.0 0.0 0.0 13.188465549438558 0.0 84-85 0.0 0.0 0.0 14.191530448912278 0.0 86-87 0.0 0.0 0.0 15.14017898285354 0.0 88-89 0.0 0.0 0.0 16.180531976814688 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTGCA 25 0.005080706 56.82809 9 CTTATGT 145 0.0 45.741104 2 CTTTAGG 75 1.3901808E-7 44.2164 2 TGAGAGA 45 9.766174E-4 42.094883 8 CCTTTAG 80 2.2711174E-7 41.538006 1 GCTTATG 175 0.0 37.977604 1 TAGGCAT 105 4.7617505E-8 36.09502 5 GTAAGCG 135 0.0 35.22876 94-95 ATGGGGC 95 8.873885E-7 34.907684 5 GGCATTC 95 8.9002606E-7 34.89444 7 TTAGGAC 70 2.0844713E-4 33.839085 2 CATTCCG 100 1.3290773E-6 33.14972 9 TACCTGG 115 1.05954314E-7 32.956326 2 GCGCCGT 30 0.0039272886 31.705883 94-95 GCTTTGC 210 0.0 31.648005 1 GGACCTA 90 2.4553323E-5 31.583143 5 CATATGC 75 3.125066E-4 31.583141 3 GGATGAC 105 1.9453655E-6 31.571161 8 TGGGGAG 60 0.004008572 31.57116 6 GGCGAGC 60 0.004008572 31.57116 8 >>END_MODULE