##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939131_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 659728 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30490899279703 33.0 31.0 34.0 31.0 34.0 2 32.47904136250091 34.0 31.0 34.0 31.0 34.0 3 32.60244070283511 34.0 31.0 34.0 31.0 34.0 4 36.0051566706279 37.0 35.0 37.0 35.0 37.0 5 36.00001818931438 37.0 35.0 37.0 35.0 37.0 6 36.12180474377319 37.0 35.0 37.0 35.0 37.0 7 36.09299893289356 37.0 35.0 37.0 35.0 37.0 8 36.12070580602915 37.0 36.0 37.0 35.0 37.0 9 37.75897036354376 39.0 38.0 39.0 35.0 39.0 10-11 37.67482659520287 39.0 37.0 39.0 35.0 39.0 12-13 37.68159984114665 39.0 37.0 39.0 35.0 39.0 14-15 38.7091384631242 40.0 38.0 41.0 34.5 41.0 16-17 38.78700919166687 40.0 38.0 41.0 34.5 41.0 18-19 38.960535250891276 40.0 38.5 41.0 35.0 41.0 20-21 39.04471085053234 40.0 39.0 41.0 35.0 41.0 22-23 39.020256833119106 40.0 38.5 41.0 35.0 41.0 24-25 38.85919424368831 40.0 38.0 41.0 34.5 41.0 26-27 38.59743409405088 40.0 38.0 41.0 34.5 41.0 28-29 38.53902365823491 40.0 38.0 41.0 34.5 41.0 30-31 38.50828447481386 40.0 38.0 41.0 34.0 41.0 32-33 38.297464257997234 40.0 37.5 41.0 34.0 41.0 34-35 38.0857345754614 40.0 37.5 41.0 34.0 41.0 36-37 37.90918454272064 40.0 37.5 41.0 33.5 41.0 38-39 37.74810603763976 40.0 37.0 41.0 33.0 41.0 40-41 37.825923714015474 40.0 37.0 41.0 33.0 41.0 42-43 37.64763508597483 40.0 37.0 41.0 33.0 41.0 44-45 37.44893501564281 40.0 37.0 41.0 33.0 41.0 46-47 37.17586414401086 40.0 37.0 41.0 32.0 41.0 48-49 36.835408683578684 40.0 36.5 41.0 31.0 41.0 50-51 36.57725532340601 39.0 36.0 41.0 31.0 41.0 52-53 36.54108208231271 40.0 36.0 41.0 31.0 41.0 54-55 36.434742045206505 39.5 36.0 41.0 31.0 41.0 56-57 36.0641052069944 39.0 35.5 40.5 29.0 41.0 58-59 35.82126649164505 39.0 35.0 40.0 28.0 41.0 60-61 35.16904618266922 38.0 35.0 40.0 28.0 41.0 62-63 34.94320083428322 38.0 34.5 40.0 27.5 41.0 64-65 34.546359257148396 37.5 34.0 39.5 26.5 41.0 66-67 33.94975959789489 37.0 33.5 39.0 26.0 40.0 68-69 33.38133594451047 36.0 33.0 39.0 25.5 40.0 70-71 32.713619855455576 35.5 32.5 37.5 24.0 39.0 72-73 32.122943091698396 35.0 31.5 37.0 22.5 39.0 74-75 31.69143495501176 35.0 31.0 37.0 21.0 38.5 76-77 29.685096736837 33.0 29.0 34.5 18.5 36.0 78-79 30.677533771493707 34.0 31.0 35.5 19.0 37.0 80-81 30.612767686076687 34.0 31.0 35.0 18.0 36.5 82-83 30.290402256687607 34.0 30.5 35.0 14.5 36.0 84-85 29.892237406931343 34.0 30.5 35.0 9.0 36.0 86-87 29.59502325200689 34.0 30.0 35.0 4.0 35.5 88-89 28.97546943588873 34.0 29.0 35.0 2.0 35.0 90-91 28.454219769359494 34.0 29.0 35.0 2.0 35.0 92-93 28.279697845172556 34.0 29.0 35.0 2.0 35.0 94-95 27.926068015909586 34.0 29.0 35.0 2.0 35.0 96-97 27.261629792884342 33.0 27.0 35.0 2.0 35.0 98-99 26.62071944801494 33.0 25.5 35.0 2.0 35.0 100-101 23.018995707321807 29.0 12.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28955390709434425 1101 2 0.28403119489154705 1101 3 0.3657743801757718 1101 4 0.20140453481761966 1101 5 0.2664907347117733 1101 6 0.23947380637611104 1101 7 0.12871633317608655 1101 8 0.21998418270863596 1101 9 0.36246980007219776 1101 10-11 0.26689542757159757 1101 12-13 0.2721822190064884 1101 14-15 0.4604708479798276 1101 16-17 0.4330892379125473 1101 18-19 0.45869590269179383 1101 20-21 0.5992062244997598 1101 22-23 0.5198152276608496 1101 24-25 0.4988612856581298 1101 26-27 0.41923346587811494 1101 28-29 0.4417685135279825 1101 30-31 0.39141524514073467 1101 32-33 0.3693547318854016 1101 34-35 0.4045905753819028 1101 36-37 0.36266290121714917 1101 38-39 0.2926865685166291 1101 40-41 0.37384684854249883 1101 42-43 0.21754330970917835 1101 44-45 0.3913074249202282 1101 46-47 0.42393987556032187 1101 48-49 0.35403667604577294 1101 50-51 0.29791743826697115 1101 52-53 0.22398229106835998 1101 54-55 0.2745251128024151 1101 56-57 0.22440706261341603 1101 58-59 0.2634646800136693 1101 60-61 0.11032993690792381 1101 62-63 0.05967268037411344 1101 64-65 0.17590622262621736 1101 66-67 0.1206086501049981 1101 68-69 0.2894168114736573 1101 70-71 0.1675560797018818 1101 72-73 0.013948623683596395 1101 74-75 -0.006772642143939578 1101 76-77 0.15837785678161254 1101 78-79 0.27359202850163555 1101 80-81 -0.05034060350562086 1101 82-83 0.08135108476561115 1101 84-85 0.2448708279891143 1101 86-87 0.47583353109676807 1101 88-89 0.4116776442681065 1101 90-91 0.49016069650805605 1101 92-93 0.643733931153136 1101 94-95 0.47690202480331934 1101 96-97 0.09617002322252333 1101 98-99 0.32341480762031694 1101 100-101 0.35703440421400856 1102 1 0.22347521587284547 1102 2 0.1075243174067424 1102 3 0.16723474405901584 1102 4 0.04217134010026058 1102 5 0.20098652727090638 1102 6 0.2532157195376641 1102 7 0.2004713158436502 1102 8 0.1056194769030867 1102 9 0.19070241377755082 1102 10-11 0.2777714500548001 1102 12-13 0.28486492769750527 1102 14-15 0.34275063463744004 1102 16-17 0.3206813674847595 1102 18-19 0.31436501309060105 1102 20-21 0.4553763761504257 1102 22-23 0.46009502737491204 1102 24-25 0.2129201441327453 1102 26-27 0.22238790409172537 1102 28-29 -0.012230350560905379 1102 30-31 0.2020069634347763 1102 32-33 0.20925328327232506 1102 34-35 0.39643029440711075 1102 36-37 0.5723919146065413 1102 38-39 0.4567979792007222 1102 40-41 0.40494894344983123 1102 42-43 0.4496168044637514 1102 44-45 0.2460935929175676 1102 46-47 0.29275356655332985 1102 48-49 0.24974175696311818 1102 50-51 0.45050979632704014 1102 52-53 0.28472415853233457 1102 54-55 0.4373423029239092 1102 56-57 0.430403563484937 1102 58-59 0.6232400437989867 1102 60-61 0.6519979636214188 1102 62-63 0.5702859418099351 1102 64-65 0.519150489212798 1102 66-67 0.8061799676966075 1102 68-69 0.43149872763819275 1102 70-71 0.429270979069301 1102 72-73 0.4954231538146132 1102 74-75 0.42494702282756336 1102 76-77 0.49872522362214866 1102 78-79 0.5153925781781616 1102 80-81 0.4178245083555119 1102 82-83 0.6184134488969306 1102 84-85 0.5134028260828742 1102 86-87 0.8961434912093154 1102 88-89 0.4749181897665693 1102 90-91 0.8790356222494999 1102 92-93 0.6255952586023632 1102 94-95 0.8062672855079747 1102 96-97 0.5501215576817557 1102 98-99 0.40265766411035386 1102 100-101 -0.09420138873681339 1103 1 0.14281306291216822 1103 2 0.17181893307795093 1103 3 0.20577682558836585 1103 4 0.16770932332160982 1103 5 0.2013591007256892 1103 6 0.2119243028592237 1103 7 0.2439660548495226 1103 8 0.30499766465440814 1103 9 0.46312426970725085 1103 10-11 0.4087599259724186 1103 12-13 0.1987587057208131 1103 14-15 0.34311678441196847 1103 16-17 0.1875987718040406 1103 18-19 0.3436891134541824 1103 20-21 0.4183599186816096 1103 22-23 0.38856092405643494 1103 24-25 0.3623799126233038 1103 26-27 0.5162584283925113 1103 28-29 0.6470383776788751 1103 30-31 0.7492627672298866 1103 32-33 0.5219066486384136 1103 34-35 0.4398447374097927 1103 36-37 0.5676608740988129 1103 38-39 0.5040023935537903 1103 40-41 0.5332330499801472 1103 42-43 0.4125939233146809 1103 44-45 0.609214602206201 1103 46-47 0.5776855397794236 1103 48-49 0.7597048850384525 1103 50-51 0.7184254405579367 1103 52-53 0.6543362967185473 1103 54-55 0.9236470166204782 1103 56-57 0.7521624956123318 1103 58-59 0.8414717330984232 1103 60-61 0.8047370208821079 1103 62-63 0.675184640372315 1103 64-65 0.6348313350830139 1103 66-67 0.47249226133587285 1103 68-69 0.7003072634245129 1103 70-71 0.61401602319998 1103 72-73 0.534646145450985 1103 74-75 0.7237509335641015 1103 76-77 0.6716731789647206 1103 78-79 0.9517371186923107 1103 80-81 0.8860851042160895 1103 82-83 0.9226196747278337 1103 84-85 0.9555479498671922 1103 86-87 1.0028183373621715 1103 88-89 1.138220989206424 1103 90-91 1.3414603045985132 1103 92-93 1.4703541778823954 1103 94-95 1.0235482701296839 1103 96-97 1.080871715061921 1103 98-99 1.139437730145783 1103 100-101 0.609949593443897 1104 1 0.17942089931948857 1104 2 0.26571853023820324 1104 3 0.21745556540447097 1104 4 0.18080010979245031 1104 5 0.21912258742715807 1104 6 0.2095156944929215 1104 7 0.18216116417023898 1104 8 0.21726711567234958 1104 9 0.3353174882703982 1104 10-11 0.17292254316627265 1104 12-13 0.18841975430846247 1104 14-15 0.3509176647583203 1104 16-17 0.4087060090700234 1104 18-19 0.40632045787915416 1104 20-21 0.4876902051253609 1104 22-23 0.40521344014895533 1104 24-25 0.3345077976845161 1104 26-27 0.39048089950426146 1104 28-29 0.39847902655797185 1104 30-31 0.46514934586191714 1104 32-33 0.32459044051056196 1104 34-35 0.4709353552780584 1104 36-37 0.4400151683464557 1104 38-39 0.6748750925801161 1104 40-41 0.5274192715359831 1104 42-43 0.7185775086486714 1104 44-45 0.7567192839387147 1104 46-47 0.821174770289268 1104 48-49 0.5044315380964832 1104 50-51 0.8188553771513227 1104 52-53 0.7658398102759989 1104 54-55 0.6909844807171623 1104 56-57 0.7965531478032659 1104 58-59 0.8649722238523765 1104 60-61 1.0962779882996543 1104 62-63 0.8133619862063242 1104 64-65 0.5320454784313497 1104 66-67 0.7299950763969036 1104 68-69 0.6863855070672855 1104 70-71 0.7743615982853385 1104 72-73 0.489416996472535 1104 74-75 0.5635528857411778 1104 76-77 0.5272319459980999 1104 78-79 0.39268707383198276 1104 80-81 0.6329283218760082 1104 82-83 0.640127547866431 1104 84-85 0.4083828819143278 1104 86-87 0.6955717380161097 1104 88-89 1.1330650619223128 1104 90-91 1.0309304709731322 1104 92-93 0.8783272737269421 1104 94-95 0.9021092470612402 1104 96-97 1.2223759512018866 1104 98-99 1.2264953741655127 1104 100-101 1.3267198302703171 1105 1 0.0022979534363258836 1105 2 0.06532276322452191 1105 3 0.061256649461945756 1105 4 -0.013785338600101227 1105 5 0.08902421924911863 1105 6 -0.0017435902585773988 1105 7 0.03351910388746404 1105 8 0.08058123423037955 1105 9 0.2152021996185809 1105 10-11 0.22441527309830178 1105 12-13 0.1975553762075748 1105 14-15 0.26550655460317074 1105 16-17 0.1854002073550376 1105 18-19 0.2722644195643156 1105 20-21 0.19967362879682327 1105 22-23 0.30177342179454314 1105 24-25 0.33068921957788433 1105 26-27 0.27611427011999723 1105 28-29 0.13317746930081142 1105 30-31 0.23719328173618948 1105 32-33 0.259592878205936 1105 34-35 0.40837118653218596 1105 36-37 0.12118038673528275 1105 38-39 0.24769622309719352 1105 40-41 0.2747464102551902 1105 42-43 0.44219160713156214 1105 44-45 0.46233602086837067 1105 46-47 0.295029770432663 1105 48-49 0.356854894086041 1105 50-51 0.4315414306659022 1105 52-53 0.24955313730000483 1105 54-55 0.321011151972435 1105 56-57 0.49646854543439645 1105 58-59 0.4976602848807943 1105 60-61 0.4545862277100241 1105 62-63 0.624165278529027 1105 64-65 0.505263809900562 1105 66-67 0.2610169009401915 1105 68-69 0.06720514628837293 1105 70-71 0.5245594817921813 1105 72-73 0.47117729932033825 1105 74-75 0.4379556503745228 1105 76-77 0.7353953576244727 1105 78-79 0.5548253628566151 1105 80-81 0.5614364523070492 1105 82-83 0.524070305043292 1105 84-85 0.5522757380329466 1105 86-87 0.6385683826001234 1105 88-89 0.6647364605858819 1105 90-91 0.41246277760653527 1105 92-93 0.5542313719230485 1105 94-95 0.35881577721696445 1105 96-97 0.203116723807927 1105 98-99 0.2620928963198601 1105 100-101 0.33765934674626763 1106 1 0.13533517617326396 1106 2 0.1230152805279161 1106 3 0.1416970004347462 1106 4 0.013545851110507101 1106 5 0.154008687067531 1106 6 -0.006027202444528257 1106 7 0.1333883065688113 1106 8 0.0227088706138332 1106 9 0.16796801791930704 1106 10-11 0.16037908584014815 1106 12-13 0.18586264090864546 1106 14-15 0.11565915147493655 1106 16-17 0.0803508858661246 1106 18-19 0.143273738873269 1106 20-21 0.2108785990949329 1106 22-23 0.2531522435286959 1106 24-25 0.3382727391157303 1106 26-27 0.2864063841316593 1106 28-29 0.23829491439318673 1106 30-31 0.20199245335317073 1106 32-33 0.14582101052922525 1106 34-35 0.09279813077981913 1106 36-37 0.23742377874574316 1106 38-39 0.312498119658315 1106 40-41 0.20810517640048687 1106 42-43 0.21953914650700312 1106 44-45 0.33512745364399876 1106 46-47 0.1615156824714603 1106 48-49 0.2523042373745099 1106 50-51 0.28746000933503524 1106 52-53 0.36050741273735554 1106 54-55 0.19711339187652044 1106 56-57 0.3914846226048141 1106 58-59 0.3378586608140921 1106 60-61 0.1285003374707756 1106 62-63 0.4402233743380535 1106 64-65 0.3557379501806892 1106 66-67 0.26554303315448635 1106 68-69 0.3163031897784805 1106 70-71 0.3690251202465902 1106 72-73 0.31997014906691135 1106 74-75 0.2527384723266728 1106 76-77 0.37239464913899667 1106 78-79 0.3822219512265548 1106 80-81 0.43451400421723463 1106 82-83 0.6251548328104768 1106 84-85 0.6603115982978096 1106 86-87 0.5309276392353652 1106 88-89 0.6286118761398605 1106 90-91 0.7974483354025992 1106 92-93 0.8826906885070578 1106 94-95 0.9420410210994667 1106 96-97 0.845770274410139 1106 98-99 1.052735438147753 1106 100-101 0.2269067171751722 1107 1 -0.0151043990117401 1107 2 -0.018553274812830978 1107 3 0.04714982002752777 1107 4 -0.024604503978771675 1107 5 0.0729867058234035 1107 6 0.06887700545043174 1107 7 0.08654087320085324 1107 8 0.024224647113832987 1107 9 0.1708802967029115 1107 10-11 0.12915160506068446 1107 12-13 0.032560484530215206 1107 14-15 0.17701206897196897 1107 16-17 0.2534103192142041 1107 18-19 0.12689465559501656 1107 20-21 0.040345045766926546 1107 22-23 0.23621530480085795 1107 24-25 0.06660334736454132 1107 26-27 0.11176905229587675 1107 28-29 0.019133511529609848 1107 30-31 0.05428127586817055 1107 32-33 0.11905637334648134 1107 34-35 -0.008786228394605189 1107 36-37 -0.3392720272746246 1107 38-39 -0.3846460083229246 1107 40-41 -0.1135214754231697 1107 42-43 -0.034451569983154684 1107 44-45 -0.21557899760018273 1107 46-47 -0.3089167362605494 1107 48-49 -0.35579671831015247 1107 50-51 -0.48430877759648183 1107 52-53 -0.5717001045201258 1107 54-55 -0.7210421317621396 1107 56-57 -0.6303475287541644 1107 58-59 -0.5021275232558438 1107 60-61 -0.32258480941045065 1107 62-63 -0.693501658835082 1107 64-65 -0.8182384037059123 1107 66-67 -0.7908103067720589 1107 68-69 -0.9516065792768131 1107 70-71 -0.7991130092355121 1107 72-73 -0.7097490098510661 1107 74-75 -0.6702451721964025 1107 76-77 -0.7822257358185389 1107 78-79 -0.5718400813054849 1107 80-81 -0.4843956803521614 1107 82-83 -0.7616787846274171 1107 84-85 -0.697057560371686 1107 86-87 -0.6979508849450013 1107 88-89 -0.5669705050704756 1107 90-91 -0.21687078276335825 1107 92-93 -0.4839160097341413 1107 94-95 -0.36231696058200313 1107 96-97 0.011904348568879897 1107 98-99 0.265688369560106 1107 100-101 -0.2566520775588721 1108 1 -0.009034818434393799 1108 2 0.030276968930387227 1108 3 -0.004066016081800683 1108 4 -0.0044685684137633075 1108 5 -0.1625917403255741 1108 6 -0.09005288597391825 1108 7 -0.10504811371438905 1108 8 -0.0060752559264827255 1108 9 -0.11187959846542839 1108 10-11 0.15478952301934612 1108 12-13 0.0188997282446266 1108 14-15 0.08120557555275099 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-0.7061053217482538 2316 52-53 -0.613202802917165 2316 54-55 -0.7369265007903181 2316 56-57 -0.9141697524379424 2316 58-59 -0.7478159342160495 2316 60-61 -0.5715407106239141 2316 62-63 -0.46483580333178054 2316 64-65 -0.7099142964423564 2316 66-67 -0.5374204112367522 2316 68-69 -0.4227335482662582 2316 70-71 -0.19463553363435437 2316 72-73 -0.1090907617446959 2316 74-75 -0.5048820304364696 2316 76-77 -0.5657766582428216 2316 78-79 -0.303773427062044 2316 80-81 -0.09585208397535538 2316 82-83 -0.2879904394372126 2316 84-85 -0.513224923104211 2316 86-87 -0.5755218526044281 2316 88-89 -0.8391121590398747 2316 90-91 -0.6393242961603463 2316 92-93 -0.45769113478849377 2316 94-95 -0.338431488079344 2316 96-97 -0.9377295317516392 2316 98-99 -0.9492105456002413 2316 100-101 -0.6187296423149178 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 3.0 5 25.0 6 44.0 7 100.0 8 153.0 9 273.0 10 515.0 11 825.0 12 1319.0 13 1876.0 14 2274.0 15 2980.0 16 4095.0 17 5665.0 18 5289.0 19 3755.0 20 3490.0 21 3815.0 22 4160.0 23 4470.0 24 5151.0 25 6033.0 26 7103.0 27 8064.0 28 9913.0 29 11919.0 30 14588.0 31 17901.0 32 21949.0 33 28738.0 34 39410.0 35 60805.0 36 93159.0 37 157453.0 38 122965.0 39 9446.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.13078213471509 20.495010805738524 15.347342518337816 16.026864541208568 2 13.474947250988286 28.67196784129217 32.30073606092208 25.552348846797468 3 29.478663934227438 25.23267164649674 31.24833264618146 14.040331773094367 4 10.708049377925448 17.00746368200228 44.853485072636 27.43100186743628 5 8.999011713918463 44.370710353357744 35.042017316227295 11.588260616496497 6 23.772221279072586 41.06525719690539 15.192624839327722 19.969896684694298 7 22.203241335823247 36.53763369146073 23.025246768365147 18.233878204350884 8 22.518371207527952 24.911327092377462 21.107183566560764 31.46311813353382 9 18.867017922537773 25.567506608784225 28.062928964664223 27.502546504013775 10-11 24.86267067639997 24.33169427400383 26.94784820410836 23.857786845487837 12-13 32.58191254577644 24.32403960420052 24.687219581403244 18.406828268619797 14-15 23.731698702936544 28.05132775824932 32.66070050824515 15.556273030568981 16-17 17.035233236019025 30.555631345514296 31.874300837630624 20.534834580836055 18-19 15.979476374715318 36.84991151649734 29.16272248769378 18.007889621093558 20-21 21.012599131763395 27.873760095069482 25.83761792738826 25.27602284577887 22-23 17.9551269614144 28.368585235127203 29.140570053112796 24.535717750345597 24-25 25.204744401076205 28.51763992572663 27.005646292015612 19.271969381181552 26-27 22.29519055724903 23.310768307304734 36.57845792855486 17.81558320689138 28-29 16.62003128562074 35.35987558508961 30.12961402274877 17.890479106540877 30-31 19.195913934323723 28.07525479172146 28.471567995112974 24.257263278841847 32-33 19.947766352193632 29.81978936773943 30.515000121262098 19.71744415880484 34-35 24.644759910226412 26.394526266960455 29.091220546357626 19.869493276455504 36-37 21.34915617092182 24.71287371901879 35.48327427818538 18.454695831874012 38-39 19.816357424576996 29.60757068905005 32.819761778602526 17.75631010777043 40-41 18.60450308958274 31.27543069207866 30.893250216786228 19.226816001552372 42-43 17.02776122887362 31.98104784123967 25.82568925554186 25.165501674344853 44-45 20.69990003160487 25.870365472022872 27.965988008340048 25.463746488032214 46-47 24.63179371201505 30.185980838797157 28.730738943958674 16.451486505229123 48-49 22.089321930619683 28.11674846830351 30.012308140046017 19.78162146103079 50-51 14.593309050561823 30.08320108165953 36.328360064087114 18.995129803691526 52-53 19.407482634875763 33.53505803532519 26.61970283185103 20.43775649794802 54-55 16.414643610700168 27.106777338539516 30.650358935803844 25.82822011495647 56-57 22.41545000363786 26.01344796643465 28.30590788931196 23.265194140615527 58-59 27.18232764435794 25.59659965105192 27.292757129409413 19.928315575180726 60-61 20.32336134504737 28.764318831769785 33.98765584626731 16.92466397691554 62-63 18.11767042065428 29.105796056835054 32.38320232651734 20.393331195993323 64-65 16.923721722680668 34.68628925173863 28.85499214813647 19.534996877444232 66-67 19.975534500674165 27.67845300433755 26.14898033892447 26.197032156063816 68-69 17.031503666054775 28.985782953186366 29.17427743780895 24.808435942949913 70-71 23.811422140201827 28.331318612608992 26.366189070487433 21.491070176701744 72-73 20.759464506584532 23.720002789028204 34.307926903208596 21.21260580117867 74-75 16.54105934566973 34.81222564450804 27.835941478912524 20.810773530909707 76-77 19.273907439490387 26.473691126218945 28.174803605322463 26.07759782896821 78-79 19.352261884280836 27.637513424123483 30.52643784962405 22.483786841971636 80-81 23.104930912354074 26.700398890110815 28.020988153047078 22.173682044488032 82-83 19.769934965474327 25.04969862511207 33.345257466541064 21.835108942872537 84-85 18.305285136666274 28.316691361205955 30.90980745838244 22.468216043745333 86-87 18.571288750566193 29.676566164370154 29.041822804479168 22.710322280584485 88-89 17.931572359585616 28.92496395241338 25.93227238659753 27.211191301403474 90-91 19.81030870105741 23.885795673285422 28.630741068181436 27.673154557475733 92-93 22.585216937889555 28.443388790531856 28.872959765236583 20.09843450634201 94-95 20.192185264230105 26.38958783013606 29.679276914122184 23.738949991511653 96-97 14.074436737564572 27.551885019280675 33.99696541605025 24.376712827104505 98-99 18.815619644986022 29.761770325463587 26.26688120196356 25.15572882758683 100-101 17.3583853186534 25.069669549954032 29.37368268276841 28.198262448624156 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2528.0 1 1533.0 2 718.5 3 897.5 4 706.5 5 646.0 6 1250.5 7 2326.5 8 3199.0 9 3275.5 10 2489.0 11 1701.0 12 1462.5 13 1374.0 14 1278.0 15 1158.5 16 1056.0 17 1014.0 18 985.0 19 1018.5 20 1104.0 21 1178.0 22 1238.5 23 1365.0 24 1477.0 25 1835.0 26 2752.5 27 3530.0 28 3631.5 29 4218.0 30 5863.0 31 7456.0 32 9987.5 33 13147.0 34 15195.0 35 16754.0 36 19163.5 37 20594.5 38 19033.0 39 17236.0 40 15943.5 41 14162.0 42 11578.5 43 10925.0 44 11600.5 45 11777.5 46 16776.0 47 52538.5 48 121112.5 49 112041.0 50 46857.5 51 20451.0 52 9067.0 53 3328.0 54 1069.5 55 672.0 56 539.5 57 462.0 58 415.5 59 359.5 60 304.5 61 270.0 62 228.0 63 190.5 64 166.0 65 138.5 66 115.5 67 105.0 68 90.5 69 75.5 70 62.5 71 48.5 72 34.0 73 27.0 74 25.0 75 16.0 76 10.5 77 8.5 78 4.5 79 6.5 80 6.5 81 2.0 82 1.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.334531807047753 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010610433390730724 16-17 0.001515776198675818 18-19 7.578880993379089E-5 20-21 0.0 22-23 0.0 24-25 4.547328596027454E-4 26-27 7.57888099337909E-4 28-29 0.0 30-31 0.00341049644702059 32-33 0.0 34-35 0.010383066960929352 36-37 0.026526083476826813 38-39 0.019932457012587005 40-41 0.013945141027817526 42-43 0.010155700531127979 44-45 0.002955763587417845 46-47 0.001439987388742027 48-49 3.0315523973516357E-4 50-51 1.5157761986758179E-4 52-53 7.578880993379089E-5 54-55 0.0 56-57 0.0 58-59 0.0046989062158950354 60-61 0.009625178861591444 62-63 0.0044715397860936625 64-65 0.0018189314384109815 66-67 0.0035620740668881722 68-69 0.004092595736424708 70-71 7.578880993379089E-5 72-73 0.0 74-75 0.0 76-77 0.2157707418815027 78-79 2.273664298013727E-4 80-81 0.00341049644702059 82-83 2.273664298013727E-4 84-85 7.578880993379089E-5 86-87 0.10936325273446026 88-89 0.3956933766643223 90-91 0.3872808187616715 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0011368321490068634 100-101 9.852545291392815E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 659728.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.026429073800111 #Duplication Level Percentage of deduplicated Percentage of total 1 72.80862006772246 9.48436325273446 2 9.3740909249584 2.442218611306478 3 3.8969501623244396 1.5229003468095943 4 2.355158891772071 1.227172410447942 5 1.5720452879367925 1.023906822205515 6 1.204342615110718 0.941297019377683 7 0.8482761028171144 0.7735005941842699 8 0.6970060158949953 0.726359954405452 9 0.5829716426767824 0.6834634879829263 >10 5.2886349620079365 14.400328620279874 >50 0.8215129335924318 7.46792617563602 >100 0.4666100373520753 10.931171634370529 >500 0.030254017384423834 2.787967162224432 >1k 0.03141763343767091 10.013520723692189 >5k 0.010472544479223635 8.9609657313317 >10k+ 0.011636160532470706 26.612937453010936 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 46478 7.045024616205467 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 25722 3.8988795382339387 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 19151 2.902862998084059 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13511 2.0479652220308977 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 13138 1.9914267698202894 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 12733 1.930037833773919 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11857 1.7972558387699173 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 11407 1.7290459098295055 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 10798 1.6367351393301481 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10778 1.6337035869327967 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9915 1.5028921009870735 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8073 1.2236861251909879 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6613 1.0023828001843185 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6314 0.9570610918439115 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6303 0.9553937380253681 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6232 0.9446317270147697 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5517 0.8362537288094487 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5079 0.7698627313074479 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5072 0.7688016879683748 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4517 0.6846761089418669 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4333 0.6567858268862319 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4261 0.645872238255766 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4066 0.6163146023815876 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3962 0.6005505299153591 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3907 0.5922137608226421 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3362 0.50960395799481 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3264 0.49474935124778696 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3141 0.47610530400407447 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3079 0.4667074915722843 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2275 0.34483908519874856 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2213 0.3354412727669585 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2049 0.3105825431086751 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2035 0.30846045643052894 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1972 0.29891106637887127 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1936 0.29345427206363833 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1833 0.27784177721727743 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1705 0.25843984187422697 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1638 0.24828414134309898 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1537 0.2329748017364732 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1517 0.22994324933912155 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1415 0.21448233211262824 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1344 0.20372032110202992 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1279 0.1938677758106371 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1219 0.1847731186185822 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1147 0.17385952998811632 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1056 0.16006596658016636 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 984 0.14915237794970046 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 880 0.13338830548347197 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 876 0.13278199500400165 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 869 0.1317209516649286 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 848 0.12853782164770935 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 833 0.12626415734969565 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 831 0.12596100210996047 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 809 0.12262629447287368 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 807 0.1223231392331385 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 794 0.12035263017485993 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 781 0.11838212111658139 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 774 0.1173210777775083 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 758 0.114895835859627 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 745 0.11292532680134844 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 723 0.10959061916426163 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 665 0.10079911721194189 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.578880993379089E-5 0.0 12-13 0.0 0.0 0.0 1.5157761986758179E-4 0.0 14-15 0.0 0.0 0.0 1.5157761986758179E-4 0.0 16-17 0.0 0.0 0.0 1.5157761986758179E-4 0.0 18-19 0.0 0.0 0.0 1.5157761986758179E-4 0.0 20-21 0.0 0.0 0.0 6.063104794703271E-4 0.0 22-23 0.0 0.0 0.0 0.0026526083476826816 0.0 24-25 0.0 0.0 0.0 0.005987315984769481 0.0 26-27 0.0 0.0 0.0 0.011671476729803797 0.0 28-29 0.0 0.0 0.0 0.031376567312589435 0.0 30-31 0.0 0.0 0.0 0.07275725753643926 0.0 32-33 0.0 0.0 0.0 0.15680704775301335 0.0 34-35 0.0 0.0 0.0 0.27859966531661534 0.0 36-37 0.0 0.0 0.0 0.44298559406300775 0.0 38-39 0.0 0.0 0.0 0.683615065602794 0.0 40-41 0.0 0.0 0.0 0.9384170446001989 0.0 42-43 0.0 0.0 0.0 1.1974631969538962 0.0 44-45 0.0 0.0 0.0 1.4996331821599205 0.0 46-47 0.0 0.0 0.0 1.902450706958019 0.0 48-49 0.0 0.0 0.0 2.2295552106322605 0.0 50-51 0.0 0.0 0.0 2.5912952004462446 0.0 52-53 0.0 0.0 0.0 2.934012198966847 0.0 54-55 0.0 0.0 0.0 3.330160308490772 0.0 56-57 0.0 0.0 0.0 3.9295740062571243 0.0 58-59 0.0 0.0 0.0 4.423489680595639 0.0 60-61 0.0 0.0 0.0 4.988874202701719 0.0 62-63 0.0 0.0 0.0 5.51628853103097 0.0 64-65 0.0 0.0 0.0 6.004671622244318 0.0 66-67 0.0 0.0 0.0 6.629171416098756 0.0 68-69 0.0 0.0 0.0 7.368870201052555 0.0 70-71 0.0 0.0 0.0 8.112434215312978 0.0 72-73 0.0 0.0 0.0 8.749590740426358 0.0 74-75 0.0 0.0 0.0 9.562577304586132 0.0 76-77 0.0 0.0 0.0 10.217013678364417 0.0 78-79 0.0 0.0 0.0 11.335959668226906 0.0 80-81 0.0 0.0 0.0 12.425878543884753 0.0 82-83 0.0 0.0 0.0 13.249854485484928 0.0 84-85 0.0 0.0 0.0 14.255950937356001 0.0 86-87 0.0 0.0 0.0 15.20619103630587 0.0 88-89 0.0 0.0 0.0 16.242981956200133 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 75 0.0 69.83965 1 ATCACGC 25 0.0050435257 56.933655 6 GTAAGCG 280 0.0 40.666897 94-95 ACAATCA 95 1.9461368E-8 39.95344 8 AGGTTAG 60 8.343972E-5 39.53726 5 TACAATC 100 3.0586307E-8 37.95577 7 CTATACA 105 4.7002686E-8 36.148354 4 GGTGGTA 145 1.6370905E-11 35.99254 5 CTTATGT 150 2.5465852E-11 34.792786 2 TGGGCGA 55 0.0025936035 34.505245 6 ATGAGAG 70 2.0664763E-4 33.88908 7 GGTACTC 170 3.6379788E-12 33.490383 8 TATGGTG 190 0.0 32.46217 2 ATGGGCG 60 0.0039723245 31.629807 5 ATGGTGG 195 0.0 31.629807 3 CCCTATA 155 1.371518E-9 30.60949 2 CAATCAA 140 1.7231287E-8 30.500172 9 TACTCCC 125 9.620635E-9 30.364614 5 ATGGGGG 110 2.7560309E-6 30.192087 5 GCTTATG 160 1.8517312E-9 29.761217 1 >>END_MODULE