##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939130_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 650934 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11473820694571 33.0 31.0 34.0 31.0 34.0 2 32.14439405531129 34.0 31.0 34.0 30.0 34.0 3 32.24880402621464 34.0 31.0 34.0 30.0 34.0 4 35.59851382782279 37.0 35.0 37.0 35.0 37.0 5 35.689667462446266 37.0 35.0 37.0 35.0 37.0 6 35.85799635600537 37.0 36.0 37.0 35.0 37.0 7 35.832757238061 37.0 36.0 37.0 35.0 37.0 8 35.85614516986361 37.0 36.0 37.0 35.0 37.0 9 37.44734489210888 39.0 38.0 39.0 35.0 39.0 10-11 37.36003880577755 39.0 37.5 39.0 34.5 39.0 12-13 37.35624349012342 39.0 37.0 39.0 35.0 39.0 14-15 38.39747424470069 40.0 38.0 41.0 34.0 41.0 16-17 38.459309238724664 40.0 38.0 41.0 34.0 41.0 18-19 38.500761213886506 40.0 38.0 41.0 34.0 41.0 20-21 38.4820504075682 40.0 38.0 41.0 34.0 41.0 22-23 38.55114727453167 40.0 38.0 41.0 34.0 41.0 24-25 38.4105923181152 40.0 38.0 41.0 34.0 41.0 26-27 38.1935841421711 40.0 38.0 41.0 34.0 41.0 28-29 38.158522215769956 40.0 38.0 41.0 34.0 41.0 30-31 38.11339475277063 40.0 38.0 41.0 34.0 41.0 32-33 37.86138610058777 40.0 37.0 41.0 33.5 41.0 34-35 37.43522384757902 40.0 37.0 41.0 33.0 41.0 36-37 37.07036274030854 39.5 36.0 41.0 32.0 41.0 38-39 36.8627902367982 39.0 36.0 41.0 31.0 41.0 40-41 36.94321083243462 40.0 36.0 41.0 31.5 41.0 42-43 36.74365373447999 40.0 36.0 41.0 31.0 41.0 44-45 36.48345608003269 39.0 35.5 41.0 30.0 41.0 46-47 36.265646747596534 39.5 35.5 41.0 30.0 41.0 48-49 36.03332135055167 39.5 35.5 41.0 28.5 41.0 50-51 34.795391698697564 38.0 34.0 40.0 26.0 40.5 52-53 35.11191073134911 38.5 35.0 39.5 27.5 40.5 54-55 35.80165423837133 39.0 35.5 41.0 28.0 41.0 56-57 35.60902718247933 39.0 35.0 41.0 27.5 41.0 58-59 35.46153066209477 39.0 35.0 41.0 27.0 41.0 60-61 34.87931188108165 39.0 35.0 40.0 26.0 41.0 62-63 34.660067687353866 38.0 35.0 40.0 26.0 41.0 64-65 34.250529239523516 37.5 34.0 39.5 24.0 41.0 66-67 33.62759895780525 37.0 33.5 39.0 22.0 41.0 68-69 33.077782847416174 36.5 33.0 39.0 21.5 40.0 70-71 32.292326103721734 35.5 32.0 38.0 19.0 39.5 72-73 31.732361499015262 35.0 31.5 37.0 15.5 39.0 74-75 31.320323565830023 35.0 31.0 37.0 12.5 39.0 76-77 30.89973330629526 35.0 31.0 36.5 7.5 38.5 78-79 30.365947238890577 34.5 31.0 36.0 2.0 37.0 80-81 29.75841406348416 34.0 30.0 35.5 2.0 37.0 82-83 29.28680941539388 34.0 29.5 35.0 2.0 36.0 84-85 28.847406956772883 34.0 29.0 35.0 2.0 36.0 86-87 28.667772001462513 34.0 29.0 35.0 2.0 36.0 88-89 28.065286956895783 34.0 28.0 35.0 2.0 35.0 90-91 27.49291710065844 34.0 26.5 35.0 2.0 35.0 92-93 26.957083974719403 33.5 25.0 35.0 2.0 35.0 94-95 26.373729133829237 33.0 25.0 35.0 2.0 35.0 96-97 25.60107783584818 33.0 21.5 35.0 2.0 35.0 98-99 24.944786260972695 33.0 10.5 35.0 2.0 35.0 100-101 22.03215763810156 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37178238306750444 1101 2 0.36148033354923825 1101 3 0.3784214197806648 1101 4 0.48517604004977954 1101 5 0.22927202213362818 1101 6 0.24149985228563509 1101 7 0.3396640141634393 1101 8 0.3108851643912729 1101 9 0.6359511502492268 1101 10-11 0.5521605527573428 1101 12-13 0.46445626724048594 1101 14-15 0.7372909011891409 1101 16-17 0.5551475421048835 1101 18-19 0.8205047613334742 1101 20-21 0.6933623408473224 1101 22-23 0.5495977034149249 1101 24-25 0.7933501904696882 1101 26-27 0.5221212544250093 1101 28-29 0.6902618293301757 1101 30-31 0.7459999012358054 1101 32-33 0.6144141741787053 1101 34-35 0.7956818208126393 1101 36-37 0.5663926046543892 1101 38-39 0.6519217900165941 1101 40-41 0.6341228024092942 1101 42-43 0.7230488199752116 1101 44-45 0.9277665464688951 1101 46-47 1.0884987096764576 1101 48-49 1.3530931950767098 1101 50-51 1.09123657698143 1101 52-53 0.96065336215824 1101 54-55 1.2338989856845117 1101 56-57 1.0155191380781545 1101 58-59 1.1528827391829068 1101 60-61 0.8023124935320638 1101 62-63 0.7673795256370184 1101 64-65 0.6476570531646573 1101 66-67 0.8354514298462874 1101 68-69 0.7088095350868073 1101 70-71 0.6817183010054606 1101 72-73 0.6890628929228058 1101 74-75 0.527901145075969 1101 76-77 0.6278544830822241 1101 78-79 0.5300176452442074 1101 80-81 0.56419996929775 1101 82-83 0.42564410104029093 1101 84-85 0.38182135664159844 1101 86-87 0.07658328051105201 1101 88-89 0.2722456423545019 1101 90-91 0.4710686603996841 1101 92-93 0.343732033731758 1101 94-95 0.39556542486596413 1101 96-97 0.41997146812907005 1101 98-99 0.18549515356087554 1101 100-101 0.07703902282377939 1102 1 0.255015584568973 1102 2 0.27709788026903936 1102 3 0.2527182326699986 1102 4 0.09957288413596643 1102 5 0.17338940280566817 1102 6 0.19819660881936585 1102 7 0.07705696367485615 1102 8 0.31982155300341475 1102 9 0.3850378875616727 1102 10-11 0.3278702811434542 1102 12-13 0.32023623886141905 1102 14-15 0.19779669273378886 1102 16-17 0.2711203303990217 1102 18-19 0.3727745839139658 1102 20-21 0.4567342173882949 1102 22-23 0.5359465647298194 1102 24-25 0.43716264707084207 1102 26-27 0.4828132207324103 1102 28-29 0.40890647705733585 1102 30-31 0.3980795236532657 1102 32-33 0.41917219730463273 1102 34-35 0.36531118208224456 1102 36-37 0.46692999559120096 1102 38-39 0.45281542887780546 1102 40-41 0.4542981085913027 1102 42-43 0.14708448502165083 1102 44-45 0.41448775730930265 1102 46-47 0.3562431772669541 1102 48-49 0.4754452364578299 1102 50-51 0.2173322406189797 1102 52-53 0.14211337483825304 1102 54-55 0.2606024847637016 1102 56-57 0.23999055270444813 1102 58-59 0.36931844850874995 1102 60-61 0.5128811819560255 1102 62-63 0.500641408115456 1102 64-65 0.6063665098362563 1102 66-67 0.5256868151274006 1102 68-69 0.442851338868671 1102 70-71 0.388015033386786 1102 72-73 0.16742636644070075 1102 74-75 -0.0375770221592866 1102 76-77 -0.180023225257397 1102 78-79 -0.25274156537479797 1102 80-81 -0.11672164609537816 1102 82-83 -0.48072508854600215 1102 84-85 -0.342589676980559 1102 86-87 -0.32721468464910686 1102 88-89 -0.10365629889478001 1102 90-91 -0.3771083364504406 1102 92-93 -0.6911078056547701 1102 94-95 -0.5850573045566954 1102 96-97 -0.7745409049450309 1102 98-99 -1.1651021152050411 1102 100-101 -1.408117313659428 1103 1 0.21905567039308949 1103 2 0.4566187172486025 1103 3 0.2597351965447885 1103 4 0.212570310709026 1103 5 0.362750194669232 1103 6 0.3278888995582676 1103 7 0.3490785623110071 1103 8 0.34030849464431867 1103 9 0.46445760247522827 1103 10-11 0.46168848181890354 1103 12-13 0.4228313811129354 1103 14-15 0.44860048351483073 1103 16-17 0.5251392843042098 1103 18-19 0.6278079995710257 1103 20-21 0.6836446789845994 1103 22-23 0.5315386798015425 1103 24-25 0.6476229469366643 1103 26-27 0.6973816045295465 1103 28-29 0.6301142375276854 1103 30-31 0.7185214141173191 1103 32-33 0.6702952356337804 1103 34-35 0.7732333829132685 1103 36-37 0.690949403249256 1103 38-39 0.8423492950466454 1103 40-41 0.8150980748839487 1103 42-43 0.8544592654376757 1103 44-45 0.885355203134452 1103 46-47 0.7496323222021033 1103 48-49 0.8878660727891656 1103 50-51 0.7660004759741099 1103 52-53 0.8791911415963156 1103 54-55 0.8919785227233703 1103 56-57 0.9443332916863554 1103 58-59 1.0270348400067206 1103 60-61 0.8478172352136397 1103 62-63 0.7472593287605633 1103 64-65 0.926215280490112 1103 66-67 0.9988100608864414 1103 68-69 0.8113570010561588 1103 70-71 0.9810602984756898 1103 72-73 0.8703527482802116 1103 74-75 0.8068149165970446 1103 76-77 0.852655083411225 1103 78-79 1.0599816648269922 1103 80-81 0.976360891549902 1103 82-83 1.029636682127304 1103 84-85 1.1391370471182576 1103 86-87 0.8500065976564777 1103 88-89 1.1352251354828304 1103 90-91 1.1748544687793867 1103 92-93 1.4821026254752674 1103 94-95 1.404647367153114 1103 96-97 1.5704879083615992 1103 98-99 1.2298071018119892 1103 100-101 1.08736946592688 1104 1 0.2007033693716238 1104 2 0.17575320767164015 1104 3 0.3396059652523533 1104 4 0.259938923537419 1104 5 0.3543513752408103 1104 6 0.2781154186939716 1104 7 0.2877969376890448 1104 8 0.22071489305078984 1104 9 0.4271032002306896 1104 10-11 0.31856543273772786 1104 12-13 0.32605973426417023 1104 14-15 0.4404081426551514 1104 16-17 0.512334576592842 1104 18-19 0.6003493863782623 1104 20-21 0.5993406939482213 1104 22-23 0.6978450211227667 1104 24-25 0.6131683540030863 1104 26-27 0.5352200168308059 1104 28-29 0.4681469825995279 1104 30-31 0.5463820532292232 1104 32-33 0.6931748829198057 1104 34-35 0.6519788434211975 1104 36-37 0.5699438526194953 1104 38-39 0.26503218040245713 1104 40-41 0.4452372657308743 1104 42-43 0.5473406131354395 1104 44-45 0.3586617752258334 1104 46-47 0.666184615936082 1104 48-49 0.7775427516421161 1104 50-51 0.8252355336059196 1104 52-53 0.8304561264747932 1104 54-55 0.948884623557781 1104 56-57 0.88059214293677 1104 58-59 0.7622108933510674 1104 60-61 0.8338526037778848 1104 62-63 0.811638131913611 1104 64-65 0.9179227367956031 1104 66-67 0.8492133013853902 1104 68-69 0.7541806562126467 1104 70-71 0.8263562308478853 1104 72-73 0.8210954638377643 1104 74-75 0.635340538158264 1104 76-77 0.7400362064117196 1104 78-79 0.8114179054595496 1104 80-81 0.7123750049673632 1104 82-83 1.0136254837014 1104 84-85 0.8755155344805523 1104 86-87 0.7837907425315542 1104 88-89 0.8968425914239511 1104 90-91 0.8813603655643938 1104 92-93 0.7281279130270413 1104 94-95 0.5442967179602007 1104 96-97 0.8149220963542625 1104 98-99 1.049815482412722 1104 100-101 0.5538349172040107 1105 1 0.19100441520705402 1105 2 0.31645878565307584 1105 3 0.22767962994977609 1105 4 0.20840943975624526 1105 5 0.22345736208557554 1105 6 0.34947304128078827 1105 7 0.30688877601478026 1105 8 0.12856472240232364 1105 9 0.16385123531901513 1105 10-11 0.2787546763983286 1105 12-13 0.2937043812152993 1105 14-15 0.3574963774397091 1105 16-17 0.36318897389481464 1105 18-19 0.4610615477522302 1105 20-21 0.3147316839403089 1105 22-23 0.4210768893625172 1105 24-25 0.5617500371571111 1105 26-27 0.6631688280664676 1105 28-29 0.38702543815874435 1105 30-31 0.5191533727374349 1105 32-33 0.4367445253953832 1105 34-35 0.25644688798037407 1105 36-37 0.2730934862017236 1105 38-39 0.32455268998013054 1105 40-41 0.3282174635791435 1105 42-43 0.08830201944048355 1105 44-45 0.13450014716441672 1105 46-47 0.2584372911611581 1105 48-49 0.4476281624977858 1105 50-51 0.3201911534554327 1105 52-53 0.25163876941815033 1105 54-55 0.34858894838468046 1105 56-57 0.2615063682620402 1105 58-59 0.05573491407142939 1105 60-61 0.049042915796043474 1105 62-63 0.09222925126358916 1105 64-65 0.4017212387850648 1105 66-67 0.4505340223246179 1105 68-69 0.5343790819733485 1105 70-71 0.37893530374451956 1105 72-73 0.27361852014887234 1105 74-75 0.2561919902583085 1105 76-77 0.37455686036204483 1105 78-79 0.290798604569634 1105 80-81 0.36435608637621186 1105 82-83 0.2390561472958197 1105 84-85 0.1887363149013126 1105 86-87 0.3566498406067815 1105 88-89 0.39329246812971164 1105 90-91 0.25669747568607093 1105 92-93 0.0036024659049651575 1105 94-95 -0.26500145774707917 1105 96-97 -0.4739343568141372 1105 98-99 -0.17122769655738423 1105 100-101 -0.4099434303644678 1106 1 0.26292486087321976 1106 2 0.19751797358276235 1106 3 0.3195476839828686 1106 4 0.3575260336255255 1106 5 0.16806108009267007 1106 6 0.19065949815605876 1106 7 0.056412360681797225 1106 8 0.09866439017640971 1106 9 0.0954385556443853 1106 10-11 0.26492738890205203 1106 12-13 0.19301913013458716 1106 14-15 0.2046893214335057 1106 16-17 0.254088457286322 1106 18-19 0.19566307340557643 1106 20-21 0.31569856815593056 1106 22-23 0.2841840729749521 1106 24-25 0.41010802041652283 1106 26-27 0.44315488694521576 1106 28-29 0.4124707951470086 1106 30-31 0.21084238454803028 1106 32-33 0.2292860255274931 1106 34-35 0.2118957302489548 1106 36-37 0.15216409685655918 1106 38-39 0.4176321599926709 1106 40-41 0.3083471497352761 1106 42-43 0.4082479413814468 1106 44-45 0.5798669729172659 1106 46-47 0.24935448202522537 1106 48-49 0.317684826409959 1106 50-51 0.33912269018296826 1106 52-53 0.2793608014489948 1106 54-55 0.28413515364520237 1106 56-57 0.3594708521313734 1106 58-59 0.2889760561190826 1106 60-61 0.20639067369273079 1106 62-63 -0.005714152281278473 1106 64-65 -0.061213929379711374 1106 66-67 0.12681753301374954 1106 68-69 0.1601794316256644 1106 70-71 0.15864450769525007 1106 72-73 0.08390543741843715 1106 74-75 -0.003771976440400948 1106 76-77 -0.04018728071310562 1106 78-79 0.07172484591967532 1106 80-81 0.10843251520060448 1106 82-83 0.039098026873759295 1106 84-85 0.10651742805487174 1106 86-87 0.034938792830335075 1106 88-89 -0.11530689889841383 1106 90-91 0.036738343408440954 1106 92-93 -0.2906802690282255 1106 94-95 -0.08999682007569731 1106 96-97 -0.1503359173202945 1106 98-99 0.17118282449202482 1106 100-101 0.1769285867696233 1107 1 0.033440081581609604 1107 2 0.01073558696703003 1107 3 0.1512812106637469 1107 4 0.13788358229607667 1107 5 0.20124579553630895 1107 6 0.13538771138666306 1107 7 0.15911020824746203 1107 8 0.14973736083880596 1107 9 0.3315678532084192 1107 10-11 0.038829798892642486 1107 12-13 0.22624983776496066 1107 14-15 0.3358401000541775 1107 16-17 0.3391875298674094 1107 18-19 0.18326953179076355 1107 20-21 0.2541583168762642 1107 22-23 0.23238604901572302 1107 24-25 0.27900864482808885 1107 26-27 0.2620268338756446 1107 28-29 0.21361951764638576 1107 30-31 0.2847924099790049 1107 32-33 0.48131509258951155 1107 34-35 0.5090593650385316 1107 36-37 0.43923379936827445 1107 38-39 0.5115941975006493 1107 40-41 0.4735943712661097 1107 42-43 0.483767112007051 1107 44-45 0.5767638530237491 1107 46-47 0.8707765604391966 1107 48-49 0.4475992268341713 1107 50-51 0.32524387147876865 1107 52-53 0.42092204680920275 1107 54-55 0.6134797920383974 1107 56-57 0.26800862524380165 1107 58-59 0.28818336725352367 1107 60-61 0.33726279377021484 1107 62-63 0.6244139765401329 1107 64-65 0.614143456965472 1107 66-67 0.5142475978356771 1107 68-69 0.15295054203938463 1107 70-71 0.15178896644837891 1107 72-73 0.3721871693218617 1107 74-75 0.39848380504801284 1107 76-77 0.3685533991055223 1107 78-79 0.46265302235652683 1107 80-81 0.5731186320532231 1107 82-83 0.5088784547634226 1107 84-85 0.5094495652306321 1107 86-87 0.29736479951813877 1107 88-89 0.25995417439689916 1107 90-91 0.49018072331472595 1107 92-93 0.49492038893058776 1107 94-95 0.4451872027797137 1107 96-97 0.2084085500901871 1107 98-99 0.1316322493191464 1107 100-101 0.1388172964994041 1108 1 -0.009627385969615432 1108 2 0.0992014547413902 1108 3 0.0707694016009981 1108 4 0.2529459895319306 1108 5 0.1802325371115785 1108 6 0.10647020050399192 1108 7 0.10775498008501216 1108 8 -0.07327013998299492 1108 9 0.1345741586801381 1108 10-11 0.11293403342032349 1108 12-13 0.15440929746672083 1108 14-15 0.17555859510841998 1108 16-17 0.22262097377062418 1108 18-19 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-1.6441234658733812 2316 76-77 -1.7489382229504677 2316 78-79 -1.663478537759378 2316 80-81 -1.5209401641036777 2316 82-83 -1.3548758417434534 2316 84-85 -1.0962660109021058 2316 86-87 -1.2666888349649703 2316 88-89 -1.7140184765527273 2316 90-91 -2.065798019974121 2316 92-93 -2.1782469235970296 2316 94-95 -1.9537632568829792 2316 96-97 -1.8073824568663426 2316 98-99 -1.937872425514957 2316 100-101 -2.038355201164155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2588.0 3 457.0 4 384.0 5 411.0 6 467.0 7 538.0 8 600.0 9 793.0 10 1029.0 11 1490.0 12 2318.0 13 3930.0 14 3284.0 15 3403.0 16 4240.0 17 6321.0 18 5184.0 19 3767.0 20 3935.0 21 4132.0 22 4507.0 23 5034.0 24 5759.0 25 6544.0 26 7519.0 27 8834.0 28 10142.0 29 12229.0 30 14580.0 31 17555.0 32 21829.0 33 28891.0 34 39856.0 35 62887.0 36 92540.0 37 132219.0 38 114728.0 39 16010.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.59685104939319 20.12537202954647 14.955356454423487 16.322420466636853 2 13.21994641830021 27.897087456284158 32.63885257650464 26.244113548910985 3 29.333835731258123 25.28240658858993 31.125550779357596 14.25820690079435 4 10.518689878148948 16.396980333406137 45.30976500373767 27.774564784707245 5 9.012812822050973 43.99289372894652 35.32247396674511 11.671819482257394 6 23.94259667786344 40.188321022006654 15.736565793215831 20.132516506914072 7 22.008228352146414 36.498860760661344 23.36824335751206 18.12466752968018 8 22.28265882801498 24.299871473990393 21.65678635779427 31.76068334020035 9 19.063168769832483 25.167423068408233 28.882401052812828 26.88700710894645 10-11 24.70970767066637 24.043415095354863 27.0327381181155 24.21413911586327 12-13 32.27835840858985 24.251970198726404 24.957336703349416 18.512334689334327 14-15 23.964856586123272 27.52762393779954 32.903444008439955 15.604075467637236 16-17 17.066856275342545 30.397601706478085 32.003535945577184 20.532006072602186 18-19 15.929389263096718 36.69679633339534 29.39695054357937 17.976863859928567 20-21 20.93843472752753 28.037655466015604 25.62584223970997 25.398067566746896 22-23 17.650441607006666 28.27061345240731 29.46281398381184 24.616130956774178 24-25 25.044193283236076 28.269783943094275 26.83291594164564 19.853106832024004 26-27 22.16245144264927 23.259701474175024 36.56895220695658 18.008894876219124 28-29 16.786969746363997 35.106688892237756 30.06879880424787 18.03754255715037 30-31 18.94110158155154 28.210767958128706 28.58924249739381 24.25888796292595 32-33 19.798272554996483 29.740191348936975 30.68817196034641 19.773364135720133 34-35 24.51241438785049 26.420909286379818 28.84305781512631 20.223618510643384 36-37 21.152056902527704 24.802664140273503 35.419074731667244 18.626204225531552 38-39 19.904197626170028 29.514464923284763 32.53312747273955 18.048209977805655 40-41 18.571670022543156 31.3887000216865 31.039940975676387 18.999688980093953 42-43 17.014167923044084 31.886202698644656 25.663216743052867 25.43641263525839 44-45 20.356692028859626 26.083220419460563 27.97947862743054 25.580608924249265 46-47 24.42176907511617 30.230674746214547 28.7260661150772 16.621490063592084 48-49 21.875364321753533 28.1229757221774 29.910400975899755 20.091258980169314 50-51 14.444253999725143 30.056654730415204 36.3915924318298 19.107498838029855 52-53 19.359570730954083 33.42060259800421 26.64332451890598 20.576502152135728 54-55 16.320759305452295 26.985329142762737 30.979881709796036 25.71402984198893 56-57 22.36899891094527 25.868793217759233 28.28941224233359 23.472795628961908 58-59 26.98926100428711 25.513430497513014 27.502710795719835 19.99459770248004 60-61 20.576791645646566 28.181595182886472 34.266317385824195 16.975295785642768 62-63 18.26653945493478 28.687866563538712 32.71773044389793 20.32786353762858 64-65 17.04262490010694 34.241526945067505 29.350608237751274 19.36523991707428 66-67 19.867552225828398 27.54098259383583 26.44940042517342 26.14206475516235 68-69 16.923575954866138 28.612195564617338 29.830516328587482 24.63371215192904 70-71 23.915696053552203 27.951263663364962 26.612149640972255 21.52089064211058 72-73 20.6709712690186 23.399673216745633 34.81662455754535 21.11273095669041 74-75 16.815854012614516 34.34752929693533 28.58420282066013 20.252413869790026 76-77 19.162582721127507 26.310182225804358 28.743796880452336 25.783438172615796 78-79 19.453663596817567 27.1894672170372 31.359002540884152 21.99786664526108 80-81 23.20663576030122 26.57453420463966 28.59410000262442 21.6247300324347 82-83 19.964782018294514 24.53169425357101 34.05807338649226 21.445450341642214 84-85 18.75357020450131 27.843195074430078 31.46596842472542 21.937266296343182 86-87 18.966631234498426 29.372418334255805 30.05628233957295 21.604668091672824 88-89 18.374419452061563 28.471321549445396 26.78016687665209 26.374092121840953 90-91 19.925981863780684 23.656616384369247 29.695897112783072 26.721504639066996 92-93 23.12763849517648 28.077411045019762 29.846279060608843 18.948671399194907 94-95 20.62116530072999 25.741922133115896 30.556984036532047 23.079928529622062 96-97 15.013968529497612 26.747301656919326 34.9250301531828 23.31369966040026 98-99 19.71568665302498 29.21485656924443 27.419559090382613 23.64989768734798 100-101 18.27022545292848 24.05909454447762 30.79983139665943 26.870848605934466 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2960.0 1 1770.0 2 730.0 3 895.0 4 721.0 5 708.0 6 1335.0 7 2311.5 8 3161.5 9 3231.5 10 2450.5 11 1824.0 12 1705.5 13 1579.5 14 1445.0 15 1487.0 16 1658.5 17 1549.0 18 1238.0 19 1112.5 20 1155.5 21 1238.5 22 1346.5 23 1543.5 24 1676.0 25 1985.0 26 2867.5 27 3677.0 28 3752.0 29 4384.5 30 6123.0 31 7509.5 32 9726.5 33 12732.5 34 14849.0 35 16397.5 36 18646.5 37 20147.0 38 18681.5 39 16953.5 40 15482.0 41 13587.5 42 11313.0 43 10543.0 44 11004.0 45 11400.5 46 18609.0 47 51339.5 48 119029.0 49 116275.5 50 46104.0 51 13356.0 52 4886.5 53 2506.0 54 1407.0 55 946.0 56 719.5 57 580.5 58 502.5 59 447.0 60 407.0 61 357.0 62 327.5 63 287.5 64 228.0 65 201.0 66 189.0 67 180.5 68 160.0 69 136.0 70 118.0 71 101.5 72 95.5 73 80.0 74 61.0 75 46.0 76 30.5 77 28.5 78 21.0 79 15.0 80 13.0 81 7.0 82 4.0 83 4.5 84 5.0 85 3.0 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3785330002734532 2 0.1093812890400563 3 0.11045666688174224 4 0.12228582314028766 5 0.12351482638792873 6 0.06897780727385572 7 0.0768127029775676 8 0.07404744567037519 9 0.07466194729419572 10-11 0.07205031539295843 12-13 0.07558369972992654 14-15 0.07012999781851922 16-17 0.07243437890784626 18-19 0.08034608731453573 20-21 0.07842576974009653 22-23 0.08940998626588871 24-25 0.10223770766314251 26-27 0.10323627280185088 28-29 0.09709125656364546 30-31 0.08603022733487574 32-33 0.08472441138425708 34-35 0.3303714355065183 36-37 0.5177944307717833 38-39 0.4987448804333465 40-41 0.4709386819554671 42-43 0.49751587718570545 44-45 0.509498658850206 46-47 0.5055043982953725 48-49 0.5110349129097574 50-51 0.511111725612735 52-53 0.5102667858799816 54-55 0.5053507728894174 56-57 0.4800025809068201 58-59 0.47032418033164647 60-61 0.4217017393468463 62-63 0.49083317202665705 64-65 0.5171799291479628 66-67 0.5274728313469568 68-69 0.5417599941007845 70-71 0.5363062921893771 72-73 0.5238626343070112 74-75 0.5263206408022933 76-77 0.503276829909023 78-79 0.480540269827663 80-81 0.48714616228373386 82-83 0.49982025827503246 84-85 0.4934448039278944 86-87 0.49428974366064765 88-89 0.5023550774732922 90-91 0.5207901261879084 92-93 0.5268583297231363 94-95 0.5212510024057738 96-97 0.5234017580891458 98-99 0.523709008901056 100-101 0.5008188234137408 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 650934.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.650947715129337 #Duplication Level Percentage of deduplicated Percentage of total 1 75.36909655230266 11.04228692924321 2 8.50180353997148 2.491189582968473 3 3.5536028856639543 1.56190950234586 4 2.170539384279842 1.2720183613085199 5 1.4438805469339822 1.0577109200011061 6 1.1009982384028185 0.967840057517352 7 0.7444845231104773 0.7635182675970221 8 0.6931046053183457 0.8123711466907552 9 0.4687106786343428 0.6180350081575091 >10 4.753166680647597 14.448008553862604 >50 0.7256102675950004 7.314873704553765 >100 0.4026507843301736 10.521343177649346 >500 0.02621424377149568 2.7399091152098367 >1k 0.02726281352235551 10.142963802781848 >5k 0.009437127757738445 9.671487431905538 >10k+ 0.009437127757738445 24.57453443820725 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 42806 6.5760891273155195 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 27175 4.1747704068308 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 19488 2.9938519112536754 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14380 2.2091333376348445 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 11646 1.789121477753505 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 11417 1.7539412597897792 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 11388 1.74948612301708 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11340 1.7421121035312335 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10324 1.5860286910808163 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 9758 1.4990767113102095 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9675 1.4863258026159334 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 7198 1.1057956720650635 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7167 1.1010332844804542 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6507 0.9996405165500649 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6062 0.9312772109000299 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5953 0.9145320416509201 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5633 0.8653719117452768 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5002 0.7684342805875864 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4900 0.7527644891801627 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4746 0.7291061766630719 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4167 0.6401570666150486 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4063 0.6241800243957145 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3899 0.5989854578190723 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3543 0.5442948132990442 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3492 0.5364599175953323 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3418 0.5250916375546523 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3374 0.5183321196926263 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3088 0.4743952535894576 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2801 0.43030476208033386 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2519 0.3869823976009857 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2041 0.31354945355443103 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2026 0.31124507246510397 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1957 0.3006449194541997 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1784 0.27406772422396125 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1712 0.2630066949951915 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1701 0.26131681552968505 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1610 0.24733690358776772 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1457 0.22383221647663204 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1449 0.22260321322899096 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1428 0.2193770797039331 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1315 0.20201740883100286 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1259 0.19341438609751527 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1175 0.18050985199728392 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1100 0.16898794655064875 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 994 0.15270365351940443 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 959 0.1473267643109747 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 907 0.13933824320130767 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 900 0.1382628653596217 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 877 0.1347294810226536 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 876 0.13457585561669846 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 846 0.1299670934380444 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 778 0.11952056583309523 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 765 0.11752343555567846 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 738 0.1133755495948898 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 728 0.11183929553533845 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 725 0.11137841931747304 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 718 0.1103030414757871 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 717 0.11014941606983196 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 701 0.10769140957454981 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 697 0.10707690795072926 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 7.681270297756762E-5 0.0 18-19 0.0 0.0 0.0 3.840635148878381E-4 0.0 20-21 0.0 0.0 0.0 9.98565138708379E-4 0.0 22-23 0.0 0.0 0.0 0.002842070010170002 0.0 24-25 0.0 0.0 0.0 0.005760952723317571 0.0 26-27 0.0 0.0 0.0 0.00937114976326325 0.0 28-29 0.0 0.0 0.0 0.028190261992767314 0.0 30-31 0.0 0.0 0.0 0.06736474051132679 0.0 32-33 0.0 0.0 0.0 0.14909345647945874 0.0 34-35 0.0 0.0 0.0 0.27621847990733317 0.0 36-37 0.0 0.0 0.0 0.4367570291304495 0.0 38-39 0.0 0.0 0.0 0.6723415891626494 0.0 40-41 0.0 0.0 0.0 0.9232118770873852 0.0 42-43 0.0 0.0 0.0 1.1905200834493206 0.0 44-45 0.0 0.0 0.0 1.4958505777851516 0.0 46-47 0.0 0.0 0.0 1.9001158335560904 0.0 48-49 0.0 0.0 0.0 2.225110379854179 0.0 50-51 0.0 0.0 0.0 2.5841329535713298 0.0 52-53 0.0 0.0 0.0 2.91949721477139 0.0 54-55 0.0 0.0 0.0 3.3009490977579907 0.0 56-57 0.0 0.0 0.0 3.9036215653199866 0.0 58-59 0.0 0.0 0.0 4.388156095702483 0.0 60-61 0.0 0.0 0.0 4.965633996687837 0.0 62-63 0.0 0.0 0.0 5.487422688014453 0.0 64-65 0.0 0.0 0.0 5.97602829165476 0.0 66-67 0.0 0.0 0.0 6.60258950984278 0.0 68-69 0.0 0.0 0.0 7.343908906279285 0.0 70-71 0.0 0.0 0.0 8.074013648081065 0.0 72-73 0.0 0.0 0.0 8.710560517656168 0.0 74-75 0.0 0.0 0.0 9.50503430455315 0.0 76-77 0.0 0.0 0.0 10.163242356367927 0.0 78-79 0.0 0.0 0.0 11.28232662604811 0.0 80-81 0.0 0.0 0.0 12.406326908718857 0.0 82-83 0.0 0.0 0.0 13.236825853312318 0.0 84-85 0.0 0.0 0.0 14.212961682751246 0.0 86-87 0.0 0.0 0.0 15.148002716097178 0.0 88-89 0.0 0.0 0.0 16.202717940682156 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGACG 40 7.67239E-6 59.203304 8 CCAGGTA 25 0.00507817 56.83518 6 GGGACGG 25 0.00507817 56.83518 9 GGGATGC 70 1.3260433E-9 54.12874 7 GGATGCC 75 2.4447218E-9 50.520153 8 CTTATGT 140 0.0 40.621532 2 ATGGGAC 190 0.0 39.88433 7 ACCCTTG 100 3.1055606E-8 37.890118 7 GACTGGT 50 0.0016384431 37.890118 7 GTAAGCG 190 0.0 37.541615 94-95 GGATATG 65 1.3503613E-4 36.4328 9 CCTGTAC 55 0.0026078091 34.466755 3 TCCGGTG 110 7.1466275E-8 34.466755 2 GGACTGG 55 0.0026156877 34.445557 6 GGGAGCC 55 0.0026156877 34.445557 7 ACTGGTA 55 0.0026156877 34.445557 8 GGATGAC 115 1.0618896E-7 32.947926 8 GCTTATG 190 0.0 32.50095 1 CATGGGG 570 0.0 31.594524 4 TAGGCAT 60 0.0039940435 31.594524 5 >>END_MODULE