##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939130_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 650934 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30132548000258 33.0 31.0 34.0 31.0 34.0 2 32.483620459217065 34.0 31.0 34.0 31.0 34.0 3 32.62604350056995 34.0 31.0 34.0 31.0 34.0 4 36.01251893433128 37.0 35.0 37.0 35.0 37.0 5 36.01095041893648 37.0 35.0 37.0 35.0 37.0 6 36.13775897402809 37.0 36.0 37.0 35.0 37.0 7 36.10375552667398 37.0 36.0 37.0 35.0 37.0 8 36.12423225703374 37.0 36.0 37.0 35.0 37.0 9 37.76171009656893 39.0 38.0 39.0 35.0 39.0 10-11 37.65787007592168 39.0 37.0 39.0 35.0 39.0 12-13 37.64770007404745 39.0 37.0 39.0 35.0 39.0 14-15 38.72966537314075 40.0 38.0 41.0 34.5 41.0 16-17 39.01226237990333 40.0 38.0 41.0 36.0 41.0 18-19 39.16650920062556 40.5 38.5 41.0 36.0 41.0 20-21 39.12674172804002 40.5 39.0 41.0 35.5 41.0 22-23 39.06530846445261 40.0 38.5 41.0 35.0 41.0 24-25 38.87725176438779 40.0 38.0 41.0 34.5 41.0 26-27 38.61787446960828 40.0 38.0 41.0 34.5 41.0 28-29 38.55703880885005 40.0 38.0 41.0 34.5 41.0 30-31 38.52028469860231 40.0 38.0 41.0 34.0 41.0 32-33 38.34246175495519 40.0 38.0 41.0 34.0 41.0 34-35 38.08730839071242 40.0 37.5 41.0 34.0 41.0 36-37 37.850428768508024 40.0 37.0 41.0 33.5 41.0 38-39 37.70177928945177 40.0 37.0 41.0 33.0 41.0 40-41 37.77615779787198 40.0 37.0 41.0 33.0 41.0 42-43 37.547105390100995 40.0 37.0 41.0 33.0 41.0 44-45 37.32535940663723 40.0 37.0 41.0 33.0 41.0 46-47 37.021005201756246 40.0 37.0 41.0 31.5 41.0 48-49 36.63686333791137 40.0 36.0 41.0 31.0 41.0 50-51 36.38568963980987 39.0 36.0 41.0 30.0 41.0 52-53 36.307607222852084 39.0 36.0 41.0 30.0 41.0 54-55 36.19629025369699 39.0 35.5 41.0 30.0 41.0 56-57 35.89673377024398 39.0 35.0 40.5 28.5 41.0 58-59 35.59612188025207 39.0 35.0 40.0 28.0 41.0 60-61 34.97053157462969 38.0 34.0 40.0 27.0 41.0 62-63 34.680585128446204 38.0 34.0 40.0 26.0 41.0 64-65 34.23146816727963 37.5 34.0 39.5 26.0 40.5 66-67 33.61555779848648 36.5 33.0 39.0 25.5 40.0 68-69 33.00205320355059 36.0 32.5 38.5 24.0 40.0 70-71 32.23683891147182 35.0 31.5 37.5 21.5 39.0 72-73 31.52673542939837 35.0 31.0 37.0 20.0 39.0 74-75 31.046517004796186 34.5 31.0 36.5 18.5 38.0 76-77 29.027677153136878 32.0 28.5 34.0 13.0 36.0 78-79 30.03811907197965 34.0 29.5 35.0 13.0 36.0 80-81 29.95354674974729 34.0 30.0 35.0 9.0 36.0 82-83 29.51818463930291 34.0 29.5 35.0 4.5 36.0 84-85 29.005933013177987 34.0 29.0 35.0 2.0 35.5 86-87 28.56691077743673 34.0 29.0 35.0 2.0 35.0 88-89 27.79150574405393 33.0 27.0 35.0 2.0 35.0 90-91 27.145549318364075 33.0 25.5 34.0 2.0 35.0 92-93 26.801584338811615 32.0 25.0 34.0 2.0 35.0 94-95 26.227026856793472 32.0 25.0 34.0 2.0 35.0 96-97 25.249463079206187 32.0 21.5 34.0 2.0 35.0 98-99 24.152215892855494 31.0 10.0 34.0 2.0 35.0 100-101 18.40692988843723 24.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1555412218821317 1101 2 0.1609235620819618 1101 3 0.2883599885813197 1101 4 0.19988373640246238 1101 5 0.28492206535195663 1101 6 0.14767647677837203 1101 7 0.1797991130951786 1101 8 0.2207761542735085 1101 9 0.17344712487241054 1101 10-11 0.3412944754823002 1101 12-13 0.31966592497016677 1101 14-15 0.2986206028570635 1101 16-17 0.22174367219194835 1101 18-19 0.27371743784404856 1101 20-21 0.20823303384581493 1101 22-23 0.317301893748656 1101 24-25 0.31333497796706666 1101 26-27 0.2703908452793584 1101 28-29 0.23502941109926923 1101 30-31 0.31393108124659364 1101 32-33 0.22799632197288844 1101 34-35 0.3692858961985195 1101 36-37 0.3393073848309811 1101 38-39 0.2927991827947878 1101 40-41 0.33225274019306994 1101 42-43 0.6113412234957778 1101 44-45 0.3560286401331254 1101 46-47 0.33364120549220644 1101 48-49 0.30855633695811235 1101 50-51 0.4830351369328838 1101 52-53 0.5584296285188586 1101 54-55 0.5253204339184521 1101 56-57 0.6707178898753838 1101 58-59 0.610795819167862 1101 60-61 0.806604802086909 1101 62-63 0.6923209331188502 1101 64-65 0.5314676845125419 1101 66-67 0.6473926366710856 1101 68-69 0.656436558280042 1101 70-71 0.6128444666057575 1101 72-73 0.5769942805931407 1101 74-75 0.2948410014209202 1101 76-77 0.42001498643165647 1101 78-79 0.4076138960048823 1101 80-81 0.5488780380348857 1101 82-83 0.3647103422598157 1101 84-85 0.15129530504740885 1101 86-87 0.39444919675723966 1101 88-89 0.6431621396965319 1101 90-91 0.9462307031168997 1101 92-93 0.9566547931180551 1101 94-95 0.8425493432922941 1101 96-97 0.785914773771907 1101 98-99 0.21068977002813938 1101 100-101 -0.8963863961002438 1102 1 0.011834432038220655 1102 2 -0.055773798261810725 1102 3 -0.04064603945559497 1102 4 0.033433435000610245 1102 5 0.00461331715080604 1102 6 -0.04868254867221822 1102 7 0.08728535125798942 1102 8 0.08019911314398342 1102 9 0.13557817177838416 1102 10-11 0.19708451060391496 1102 12-13 0.02002016019443431 1102 14-15 0.027363973525986296 1102 16-17 0.2207071316209479 1102 18-19 0.050076262024632 1102 20-21 -0.013380346584696667 1102 22-23 -0.07155177288781545 1102 24-25 -0.04804533178748471 1102 26-27 -0.20778645347578362 1102 28-29 -0.08905556510055845 1102 30-31 0.18689448029458333 1102 32-33 0.11759971941072678 1102 34-35 0.12702724410380029 1102 36-37 0.08621537424417625 1102 38-39 0.09553444228215113 1102 40-41 0.21388584508493125 1102 42-43 0.33210926986838274 1102 44-45 0.37244724150779973 1102 46-47 0.20892424144910393 1102 48-49 0.14637295984854148 1102 50-51 0.007137221083873158 1102 52-53 -0.013886687434812472 1102 54-55 0.16234505930463428 1102 56-57 0.16816679245064137 1102 58-59 -0.023612296039104308 1102 60-61 0.2242954299487181 1102 62-63 0.04157975629308197 1102 64-65 0.16806964286978143 1102 66-67 0.11174268547905797 1102 68-69 -0.0158606370223211 1102 70-71 -0.19173241350775783 1102 72-73 -0.01908699223842447 1102 74-75 0.04788269450427407 1102 76-77 -0.03135817823740439 1102 78-79 0.24296496122643063 1102 80-81 0.43211627128670926 1102 82-83 0.5305769606216764 1102 84-85 0.023363052534048023 1102 86-87 0.11888035738077818 1102 88-89 0.30406242046820253 1102 90-91 0.7118869943143586 1102 92-93 0.33076039949428804 1102 94-95 0.4962844115731464 1102 96-97 0.076905927954801 1102 98-99 0.2747942694192993 1102 100-101 -0.30969940116218453 1103 1 0.24599035448537165 1103 2 0.2399952804254184 1103 3 0.3652099653152945 1103 4 0.21167404069586127 1103 5 0.18174871052212893 1103 6 0.24073074887945722 1103 7 0.2942679595590718 1103 8 0.19961390159792103 1103 9 0.20132047904685635 1103 10-11 0.25403049456699023 1103 12-13 0.4148305239097567 1103 14-15 0.26600556710603485 1103 16-17 0.4451089881183634 1103 18-19 0.3630409535699144 1103 20-21 0.4753892701237845 1103 22-23 0.47592212011726076 1103 24-25 0.5812596484742656 1103 26-27 0.5755903104884581 1103 28-29 0.4119183830030835 1103 30-31 0.622466435774939 1103 32-33 0.6898744473210598 1103 34-35 0.7927734406825095 1103 36-37 0.865848677111245 1103 38-39 0.783111013077324 1103 40-41 0.8409463692343095 1103 42-43 0.8824952839087956 1103 44-45 1.0086865378019354 1103 46-47 0.9158112375403462 1103 48-49 0.9168793480406023 1103 50-51 0.7147696683026936 1103 52-53 0.7923170864925524 1103 54-55 0.7733346294710373 1103 56-57 0.6006969832213045 1103 58-59 0.6024888649200051 1103 60-61 0.7364479038741365 1103 62-63 0.6581034908087631 1103 64-65 0.7835124732504752 1103 66-67 0.7905229329688339 1103 68-69 0.7053001276160771 1103 70-71 0.6278084534194903 1103 72-73 0.7087488010836296 1103 74-75 0.6913350407306744 1103 76-77 0.8551355437676627 1103 78-79 0.8039801360185166 1103 80-81 0.8479759060804781 1103 82-83 0.6034869425363851 1103 84-85 0.4858263471687465 1103 86-87 0.8590225764915509 1103 88-89 0.967850997564053 1103 90-91 0.8169486074705965 1103 92-93 0.800603660291987 1103 94-95 1.008952219104149 1103 96-97 0.873349153748709 1103 98-99 1.2802846790284867 1103 100-101 0.1030892990951564 1104 1 0.18327552568040062 1104 2 0.08510598937565561 1104 3 0.0847634857748858 1104 4 0.04647296700730408 1104 5 0.14235988708559688 1104 6 0.14271415771916907 1104 7 0.14254872182936396 1104 8 0.0217994165190305 1104 9 0.28181675635972425 1104 10-11 0.26582102813191 1104 12-13 0.15577583138063744 1104 14-15 -0.1109514246226766 1104 16-17 0.1249093111965962 1104 18-19 0.2448501916639998 1104 20-21 0.2901081556253402 1104 22-23 0.13338024747809385 1104 24-25 0.07098430322995597 1104 26-27 0.3906037315954265 1104 28-29 0.222607899964693 1104 30-31 0.37011622383076315 1104 32-33 0.22909626603287592 1104 34-35 0.28652139107906294 1104 36-37 0.376212079392225 1104 38-39 0.20723924841431085 1104 40-41 0.29377512562604124 1104 42-43 0.12959243814828625 1104 44-45 0.12027605840728484 1104 46-47 -0.07881424677335502 1104 48-49 -0.08099513728119945 1104 50-51 -0.04740345790429501 1104 52-53 -0.05566914017233415 1104 54-55 -0.03135182674943593 1104 56-57 0.08035277130142049 1104 58-59 0.13497887500101768 1104 60-61 -0.005988300321021711 1104 62-63 0.10314375412966825 1104 64-65 0.3610360664634058 1104 66-67 -0.01873034336021817 1104 68-69 0.025202232482897102 1104 70-71 0.06345697831230268 1104 72-73 -0.02360031773871185 1104 74-75 -0.3416620564235693 1104 76-77 -0.27397429068510704 1104 78-79 -0.2294359911722843 1104 80-81 0.19339025998474213 1104 82-83 -0.07567268968884733 1104 84-85 0.05243390428436001 1104 86-87 0.09571381815649715 1104 88-89 0.5191214480517061 1104 90-91 0.5728939756704712 1104 92-93 -0.10079371378645163 1104 94-95 0.3017995585670583 1104 96-97 0.17099104703572365 1104 98-99 0.14564850641240312 1104 100-101 0.36994463049326143 1105 1 0.1146609833128025 1105 2 0.22567008216684314 1105 3 0.03963837244236146 1105 4 0.02044257945338046 1105 5 0.13751762863546446 1105 6 0.11652784771068525 1105 7 0.09803863595652018 1105 8 0.07455895521956535 1105 9 0.15598444807215373 1105 10-11 0.02679334900876995 1105 12-13 0.10639316880261163 1105 14-15 0.034053570670771194 1105 16-17 0.289336758168524 1105 18-19 0.2521742158493012 1105 20-21 0.2279860510186964 1105 22-23 0.10774977214560977 1105 24-25 0.10152056097555118 1105 26-27 0.2506705472546642 1105 28-29 0.1650104339933236 1105 30-31 0.1710465611430081 1105 32-33 0.14701175606250416 1105 34-35 0.14847371345740612 1105 36-37 0.1496625278136534 1105 38-39 0.16862534128634366 1105 40-41 0.010988098374255628 1105 42-43 -0.12281569753890409 1105 44-45 -0.027879147958969952 1105 46-47 0.030330027696358286 1105 48-49 -0.05914145029263551 1105 50-51 0.04624385705329104 1105 52-53 -0.05343713910975367 1105 54-55 -0.14674021803394766 1105 56-57 -0.07412884428620714 1105 58-59 -0.18162455644796438 1105 60-61 -0.43790071810080633 1105 62-63 -0.22909234711598003 1105 64-65 0.09992081666985797 1105 66-67 -0.07777586694204786 1105 68-69 0.09175080232748911 1105 70-71 0.039745215406831846 1105 72-73 -0.1962526741889974 1105 74-75 0.020490943928702876 1105 76-77 -0.0198004775726055 1105 78-79 -0.18652844163803195 1105 80-81 9.631826067817428E-4 1105 82-83 0.09734348582208696 1105 84-85 0.1244282102273182 1105 86-87 0.1130645224062512 1105 88-89 0.11215373554961516 1105 90-91 0.36630249827633676 1105 92-93 0.46608258195801966 1105 94-95 0.12715919082106453 1105 96-97 0.17598778421399075 1105 98-99 0.12362879088425771 1105 100-101 -0.6718021629903355 1106 1 -0.0113487642292327 1106 2 -0.006862760978677329 1106 3 -0.08756311610961376 1106 4 -0.08347498804582187 1106 5 -0.031656191287972035 1106 6 -0.016295060699526687 1106 7 -0.05542050475324345 1106 8 0.08887390088853664 1106 9 -8.644088121201321E-4 1106 10-11 -0.14987522894065108 1106 12-13 -0.050856214246564946 1106 14-15 -0.15092908724896148 1106 16-17 -0.19092924741917017 1106 18-19 -0.040779053287415934 1106 20-21 0.03664416188433961 1106 22-23 0.08409062131729428 1106 24-25 0.0047885734101313915 1106 26-27 -0.202654803662476 1106 28-29 -0.11863516921313533 1106 30-31 -0.06988468327070052 1106 32-33 -0.17561839386076628 1106 34-35 -0.29350480147590474 1106 36-37 -0.3546027245037706 1106 38-39 -0.22536311231726813 1106 40-41 -0.14809667825862505 1106 42-43 -0.0560458778178301 1106 44-45 -0.10761617793800582 1106 46-47 -0.5513829313628378 1106 48-49 -0.605696644203519 1106 50-51 -0.8827758797518257 1106 52-53 -0.6169296710534695 1106 54-55 -0.5759075171103518 1106 56-57 -0.8415004370030985 1106 58-59 -0.6792410744280772 1106 60-61 -0.6203284406949763 1106 62-63 -0.6558677320427009 1106 64-65 -0.3717233362102732 1106 66-67 -0.3955856493368799 1106 68-69 -0.6170624597262346 1106 70-71 -0.8193398422970404 1106 72-73 -1.1875872554775384 1106 74-75 -0.7448119662803592 1106 76-77 -0.9711611422492901 1106 78-79 -0.9418167923696572 1106 80-81 -1.1303939405753916 1106 82-83 -0.9291474552598125 1106 84-85 -0.9983042495373695 1106 86-87 -1.2547618431771674 1106 88-89 -0.94094057501135 1106 90-91 -0.7918997154388023 1106 92-93 -1.1825604072882 1106 94-95 -1.00680486582036 1106 96-97 -1.5078298344401269 1106 98-99 -1.20558685240292 1106 100-101 -1.6298087512944086 1107 1 0.019942155289854213 1107 2 0.13984690387355414 1107 3 0.11481873807892384 1107 4 0.07554435031599382 1107 5 0.054695492026063164 1107 6 0.0869059954975171 1107 7 -0.009422907168641359 1107 8 -0.014987278459344111 1107 9 0.07651609266504522 1107 10-11 0.06926401375741875 1107 12-13 0.051644137458396244 1107 14-15 0.0340136118972012 1107 16-17 0.0872693738973993 1107 18-19 0.14605473636055422 1107 20-21 0.01828516310719408 1107 22-23 0.0512521999855764 1107 24-25 0.16831785149512513 1107 26-27 0.21287919267979305 1107 28-29 0.15970142866364512 1107 30-31 0.01907232215096144 1107 32-33 0.12915018427725755 1107 34-35 -0.2325056347461043 1107 36-37 0.05163814469444361 1107 38-39 0.017505393315424556 1107 40-41 0.32225729925389857 1107 42-43 0.2899019399881766 1107 44-45 0.07820037275619995 1107 46-47 0.1321367154171682 1107 48-49 0.05618232600436812 1107 50-51 0.12350070102667843 1107 52-53 0.10978204992004947 1107 54-55 0.09130384133652569 1107 56-57 -0.10803868626992852 1107 58-59 0.14958682821999503 1107 60-61 0.32144274547057705 1107 62-63 -0.09615364111885327 1107 64-65 -0.2734704982218048 1107 66-67 -0.3282043903413552 1107 68-69 0.06795328583416449 1107 70-71 -0.0404134622609007 1107 72-73 -0.25244435504791696 1107 74-75 -0.40227839198403714 1107 76-77 -0.1355155742728371 1107 78-79 -0.17975060690751832 1107 80-81 0.1577931015318086 1107 82-83 0.019790395768790603 1107 84-85 -0.13213880752540064 1107 86-87 -0.14284662030444295 1107 88-89 -0.47403871692700505 1107 90-91 0.020067992635262044 1107 92-93 0.22531994809612854 1107 94-95 0.2195686773094856 1107 96-97 -0.07536530253237217 1107 98-99 -0.3189711198285501 1107 100-101 -1.2438423201798834 1108 1 -0.018094880640376232 1108 2 0.028833745061326965 1108 3 0.09409435592549897 1108 4 0.13634782480024654 1108 5 -0.004001177927818844 1108 6 0.148715556305973 1108 7 0.09552384994617569 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-0.18048506148282684 2316 62-63 -0.17532667841195604 2316 64-65 -0.3249953426872807 2316 66-67 -0.5010324792316325 2316 68-69 -0.29603172184969395 2316 70-71 0.018469700124576605 2316 72-73 -2.5650922555087163E-4 2316 74-75 -0.23361679053564544 2316 76-77 -0.4493570717838935 2316 78-79 -0.3606447886844606 2316 80-81 -0.5616943677229962 2316 82-83 -0.5069134453767852 2316 84-85 -0.34252100600739865 2316 86-87 -0.37972345572916666 2316 88-89 -0.6641740544496351 2316 90-91 -1.2714287363494918 2316 92-93 -1.3071800225761763 2316 94-95 -1.2146414888416643 2316 96-97 -0.8540008609902863 2316 98-99 -0.5938165628917709 2316 100-101 -0.020948928295442926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 2.0 5 15.0 6 48.0 7 92.0 8 171.0 9 311.0 10 537.0 11 858.0 12 1381.0 13 2004.0 14 2336.0 15 3139.0 16 3997.0 17 5892.0 18 5646.0 19 4025.0 20 3977.0 21 4246.0 22 4863.0 23 5226.0 24 5962.0 25 6821.0 26 7740.0 27 9072.0 28 10908.0 29 13420.0 30 16503.0 31 20064.0 32 24895.0 33 32936.0 34 45623.0 35 68721.0 36 106367.0 37 151475.0 38 78666.0 39 2993.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.17557321386975 20.455516629102895 15.352088992967014 16.016821164060342 2 13.415031324220275 28.661277487425764 32.33784070274406 25.585850485609907 3 29.470422500591457 25.260625501202888 31.26000485456283 14.008947143642828 4 10.715832941588548 16.996807664064253 44.827739832302505 27.45961956204469 5 8.948065395262807 44.44475169525636 35.032430323197126 11.57475258628371 6 23.73973398224705 40.96682612983805 15.280043752515615 20.01339613539929 7 22.187963756694227 36.52090688149644 23.030599108358143 18.260530253451197 8 22.517490252467994 24.784079491930058 21.13470797346582 31.56372228213613 9 18.768262220132918 25.632245358208362 28.15815428292269 27.441338138736032 10-11 24.927872871904064 24.30745666995425 26.916476939290312 23.848193518851375 12-13 32.627808656484376 24.305766790488743 24.682840349405623 18.383584203621258 14-15 23.69062442150122 28.052303586942077 32.65202659884581 15.605045392710895 16-17 17.011101176485045 30.653135091454892 31.806115018389303 20.529648713670763 18-19 15.916052933169874 36.840831789398 29.174616781424845 18.068498496007276 20-21 21.015110594929748 27.80420134760206 25.84186722463414 25.338820832834053 22-23 17.873701481256163 28.377608175329605 29.2121013804779 24.536588962936335 24-25 25.218455327268202 28.493672169528704 27.025781415627392 19.2620910875757 26-27 22.272911628878777 23.266207210169085 36.64139963190222 17.81948152904992 28-29 16.62296023867243 35.36533657790191 30.09583152823481 17.91587165519085 30-31 19.16323313884357 28.00921445184919 28.491060537627472 24.336491871679772 32-33 19.983131930426126 29.737039392626595 30.59588222461878 19.6839464523285 34-35 24.677729123453947 26.30590765921487 29.0832757163709 19.933087500960283 36-37 21.31127891645691 24.679679264361035 35.5224093897233 18.48663242945875 38-39 19.71018657094828 29.57169505745254 32.85481823853178 17.8633001330674 40-41 18.552435589076012 31.2921208918087 30.932146426489084 19.223297092626208 42-43 17.098691978487206 31.933793078018063 25.72232332644766 25.24519161704707 44-45 20.69035710801578 25.820102487365755 28.033313386884046 25.456227017734417 46-47 24.67807911383897 30.174797107071388 28.695455121582125 16.45166865750752 48-49 22.03426545279129 28.161919012840325 30.004701052974415 19.79911448139397 50-51 14.468057649779814 30.094610714968802 36.36509581284024 19.072235822411148 52-53 19.42501083059112 33.484577545496165 26.659308009721418 20.4311036141913 54-55 16.395901888670743 27.054893430055888 30.629372563116995 25.91983211815637 56-57 22.425315008894913 25.957239904506444 28.32391609594828 23.29352899065036 58-59 27.2247604943127 25.60368030676392 27.278837467968607 19.892721730954765 60-61 20.29975964843687 28.716519580318057 33.97252971364443 17.011191057600648 62-63 18.078308380439267 29.093376902000458 32.38069121475313 20.44762350280715 64-65 16.84070319257294 34.75750364485795 28.781165647847573 19.62062751472154 66-67 19.929039389360824 27.709818880684317 26.122153951802396 26.238987778152456 68-69 17.008894518644382 28.97774104268432 29.202043611486445 24.811320827184858 70-71 23.758340905791453 28.339838094060298 26.36736317918804 21.534457820960206 72-73 20.751258960201802 23.70263344670888 34.292877618929104 21.253229974160206 74-75 16.423784899851597 34.878497666430086 27.762031173667378 20.935686260050943 76-77 19.19308645392443 26.44638964813341 28.18184963498599 26.17867426295617 78-79 19.389704724333832 27.622775445295066 30.491793203320363 22.49572662705074 80-81 23.08518932454594 26.655682424828996 27.983162616707567 22.275965633917497 82-83 19.729880448709086 24.998156495128537 33.42474044987664 21.847222606285737 84-85 18.202152599188242 28.280977794983826 30.874558711021393 22.64231089480654 86-87 18.496444455380377 29.66611406039505 28.964616794719582 22.87282468950499 88-89 17.840736958687987 28.92419395143501 25.83581937601614 27.399249713860858 90-91 19.796438287064284 23.847078813831892 28.604693674022048 27.75178922508178 92-93 22.611201750100623 28.314544946185023 28.846933790522538 20.227319513191812 94-95 20.126925310400132 26.310040649282417 29.673822538076056 23.889211502241395 96-97 14.057262333815718 27.451323790123116 33.98017310510743 24.511240770953737 98-99 18.7397791018591 29.651953694194567 26.27503423928865 25.333232964657682 100-101 17.745261982251588 24.47131533809625 29.158751727332294 28.62467095231987 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2307.0 1 1434.0 2 707.5 3 859.5 4 694.5 5 642.0 6 1178.5 7 2192.0 8 3068.5 9 3159.5 10 2442.5 11 1737.5 12 1522.0 13 1424.5 14 1328.0 15 1234.5 16 1111.5 17 1030.5 18 1000.0 19 979.0 20 1056.0 21 1200.0 22 1291.0 23 1395.0 24 1501.5 25 1812.5 26 2648.0 27 3455.5 28 3590.5 29 4154.0 30 5796.0 31 7285.5 32 9778.5 33 13037.5 34 14931.5 35 16399.5 36 18809.0 37 20300.5 38 18790.5 39 16782.5 40 15510.0 41 13794.0 42 11394.0 43 10816.0 44 11307.0 45 11544.0 46 17110.0 47 52023.5 48 117824.0 49 110201.0 50 47685.0 51 20836.0 52 9230.5 53 3449.0 54 1116.0 55 672.0 56 514.0 57 433.5 58 394.0 59 351.0 60 279.0 61 238.5 62 226.5 63 194.0 64 133.5 65 107.5 66 113.0 67 109.0 68 80.0 69 64.0 70 57.5 71 47.5 72 42.0 73 34.5 74 26.5 75 17.0 76 13.0 77 11.0 78 6.0 79 5.5 80 5.0 81 2.0 82 1.5 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41524947229673054 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.376889208429734E-4 16-17 0.0018435048714616228 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.003072508119102705 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01290453410023136 36-37 0.02765257307192434 38-39 0.020893055209898394 40-41 0.00837258462455487 42-43 0.010062464090061358 44-45 0.0037638224459008132 46-47 0.005607327317362436 48-49 0.002458006495282164 50-51 5.376889208429734E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0015362540595513525 60-61 0.0019203175744391904 62-63 0.0011521905446635144 64-65 0.0026116319012372993 66-67 0.0014594413565737847 68-69 0.004224698663766219 70-71 7.681270297756762E-5 72-73 0.0 74-75 0.0 76-77 0.09471006277134086 78-79 0.015592978704446226 80-81 2.3043810893270286E-4 82-83 0.0 84-85 0.0 86-87 0.10477252686140223 88-89 0.40680007496919807 90-91 0.4114856498508297 92-93 0.0 94-95 0.0 96-97 0.0 98-99 7.681270297756762E-5 100-101 3.840635148878381E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 650934.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.06691615432594 #Duplication Level Percentage of deduplicated Percentage of total 1 72.77943026441093 9.51002713024669 2 9.340794996296601 2.441107700627099 3 3.9385353351282086 1.543935329849109 4 2.3360805107163434 1.2210147265314149 5 1.5895223203263693 1.038507744256714 6 1.101614211646308 0.8636820322797704 7 0.8970455106575591 0.8205132932063773 8 0.7830043382672797 0.8185161629289605 9 0.6219358782933797 0.7314105577523988 >10 5.241191201194493 14.367816091954023 >50 0.8218018505237665 7.44668430286327 >100 0.4667458292674324 11.010025593992632 >500 0.028216372550172238 2.6349829629424795 >1k 0.032919101308534274 10.686029612833252 >5k 0.00940545751672408 8.243539283552556 >10k+ 0.011756821895905099 26.622207474183252 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 45749 7.0282086970414825 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 25568 3.927894379460898 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 18735 2.878171980569459 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13448 2.065954459284659 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 12876 1.9780807270783214 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 12371 1.9004998970709779 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11912 1.8299858357375711 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 11352 1.743955608402695 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 10661 1.6378004528876968 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10621 1.6316554366494915 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9862 1.5150537535295436 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7955 1.2220901043731007 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6622 1.0173074382349057 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6356 0.9764430802508396 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6288 0.9659965526458903 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5947 0.9136102892151893 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5499 0.8447861073472887 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5131 0.7882519579557989 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4849 0.7449295934764508 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4450 0.6836330565003518 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4372 0.6716502748358513 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4160 0.6390816887733626 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3928 0.6034405945917711 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3882 0.5963738259178349 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3854 0.5920723145510911 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3382 0.5195611229402675 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3276 0.503276829909023 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3098 0.47593150764900893 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2962 0.45503845243911056 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2273 0.34919054773602237 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2163 0.3322917530809575 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2037 0.31293495193061044 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2031 0.31201319949487966 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1914 0.29403902699812884 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1827 0.2806736166800321 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1731 0.2659255777083391 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1645 0.2527137927961975 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1638 0.2516384149545115 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1487 0.2284409786552861 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1487 0.2284409786552861 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1352 0.20770154885134282 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1276 0.19602601799875255 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1227 0.18849837310695094 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1115 0.1712923276399758 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1104 0.1696024481744693 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1039 0.15961679678738552 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 988 0.15178190108367362 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 878 0.13488310642860873 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 875 0.13442223021074334 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 841 0.12919896640826875 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 835 0.1282772139725379 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 818 0.12566558207130063 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 809 0.12428295341770441 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 798 0.12259307395219791 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 774 0.11890606420927466 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 772 0.1185988133973644 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 744 0.11429730203062062 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 738 0.1133755495948898 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 733 0.11260742256511413 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 732 0.11245379715915899 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 725 0.11137841931747304 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 658 0.10108551711847898 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 7.681270297756762E-5 0.0 18-19 0.0 0.0 0.0 3.840635148878381E-4 0.0 20-21 0.0 0.0 0.0 0.0010753778416859466 0.0 22-23 0.0 0.0 0.0 0.0029956954161251374 0.0 24-25 0.0 0.0 0.0 0.005760952723317571 0.0 26-27 0.0 0.0 0.0 0.009294337060285682 0.0 28-29 0.0 0.0 0.0 0.02803663658681218 0.0 30-31 0.0 0.0 0.0 0.06820968024408004 0.0 32-33 0.0 0.0 0.0 0.15116739945985308 0.0 34-35 0.0 0.0 0.0 0.28105768019491995 0.0 36-37 0.0 0.0 0.0 0.4434397342894979 0.0 38-39 0.0 0.0 0.0 0.682019989737823 0.0 40-41 0.0 0.0 0.0 0.9371917890293024 0.0 42-43 0.0 0.0 0.0 1.2070348145894974 0.0 44-45 0.0 0.0 0.0 1.5150537535295436 0.0 46-47 0.0 0.0 0.0 1.9230060190434055 0.0 48-49 0.0 0.0 0.0 2.2512266988665517 0.0 50-51 0.0 0.0 0.0 2.612400028267075 0.0 52-53 0.0 0.0 0.0 2.950606359477305 0.0 54-55 0.0 0.0 0.0 3.3342089981472776 0.0 56-57 0.0 0.0 0.0 3.9409525389670845 0.0 58-59 0.0 0.0 0.0 4.429097266389526 0.0 60-61 0.0 0.0 0.0 5.0097244881969605 0.0 62-63 0.0 0.0 0.0 5.53550744007841 0.0 64-65 0.0 0.0 0.0 6.029873996442035 0.0 66-67 0.0 0.0 0.0 6.6600454116700005 0.0 68-69 0.0 0.0 0.0 7.402977874869034 0.0 70-71 0.0 0.0 0.0 8.135386997760142 0.0 72-73 0.0 0.0 0.0 8.777080318434741 0.0 74-75 0.0 0.0 0.0 9.577084619946108 0.0 76-77 0.0 0.0 0.0 10.237673865553191 0.0 78-79 0.0 0.0 0.0 11.356911760639328 0.0 80-81 0.0 0.0 0.0 12.480527979795188 0.0 82-83 0.0 0.0 0.0 13.307877603566567 0.0 84-85 0.0 0.0 0.0 14.282477178945946 0.0 86-87 0.0 0.0 0.0 15.214829767687661 0.0 88-89 0.0 0.0 0.0 16.267010173074382 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGGGA 70 1.3042154E-9 54.23484 6 GCGAGCT 35 2.7999782E-4 54.23484 9 TTACTCG 35 7.980816E-8 47.455486 94-95 GGCGAGC 40 5.411404E-4 47.455482 8 GTAAGCG 640 0.0 45.231007 94-95 GGGTCCT 80 2.2772838E-7 41.52355 7 GGGGTCC 80 2.2772838E-7 41.52355 6 CATATAG 70 4.33515E-6 40.676132 3 AGCATCC 30 8.33729E-5 39.546238 94-95 CTCCGAC 150 0.0 37.96439 94-95 TGGGACC 50 0.0016227585 37.964386 6 AGGGGTC 90 5.7348734E-7 36.90982 5 GCTTATG 95 8.486222E-7 35.107433 1 GCTTTGC 190 0.0 35.107433 1 CATGGGG 475 0.0 33.968136 4 TGGGAAC 115 1.0437725E-7 33.01251 6 GCCTTAA 135 1.2065357E-8 31.636992 7 GCTTGTC 45 2.4287212E-5 31.63699 94-95 CTTATGT 90 2.4268691E-5 31.63699 2 GATGGAG 75 3.09396E-4 31.63699 7 >>END_MODULE