FastQCFastQC Report
Fri 27 May 2016
SRR939129_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939129_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436693
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75520.5256516179865844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63690.4433097397982729No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36760.2558653797296987No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15710.10934834373105458No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15280.10635535914770936No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78100.027.6634031
GTATCAA108600.027.1960471
GAGTACT61800.023.3737212-13
GTACACG1903.2989556E-722.4923151
ATCAACG129050.022.4705753
TCAACGC130450.022.4110344
TATCAAC129800.022.3780212
CAACGCA131200.022.3190365
GTACATG80100.021.933831
AACGCAG133800.021.911586
TACATGG80350.021.8198492
GTACTTT66100.021.71178214-15
GTCGGGA3101.0913936E-1121.3994352
ACATGGG80850.020.8636743
AGTACTT64250.020.2710412-13
ACGCAGA146850.019.8353717
CGCAGAG147800.019.6117428
CATGGGG31450.019.4352384
GTAGCCC7500.018.9531233
GCAGAGT151950.018.5449289
Produced by FastQC (version 0.11.5)