##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939129_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1436693 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86876597853543 33.0 31.0 34.0 30.0 34.0 2 31.976593468472387 34.0 31.0 34.0 30.0 34.0 3 32.013548475561585 34.0 31.0 34.0 30.0 34.0 4 35.47074218361195 37.0 35.0 37.0 33.0 37.0 5 35.525967621475154 37.0 35.0 37.0 33.0 37.0 6 35.612162097260864 37.0 35.0 37.0 33.0 37.0 7 35.53603031406153 37.0 35.0 37.0 33.0 37.0 8 35.517862201597694 37.0 35.0 37.0 33.0 37.0 9 37.15699039391157 39.0 37.0 39.0 34.0 39.0 10-11 37.060512579931824 39.0 37.0 39.0 33.0 39.0 12-13 36.958018170896636 39.0 37.0 39.0 33.0 39.0 14-15 38.193271979469515 40.0 38.0 41.0 33.0 41.0 16-17 38.12647865619168 40.0 38.0 41.0 33.0 41.0 18-19 38.0816141653088 40.0 38.0 41.0 33.0 41.0 20-21 37.99507967255357 40.0 38.0 41.0 33.0 41.0 22-23 37.908512813802254 40.0 38.0 41.0 33.0 41.0 24-25 37.938315283780184 40.0 38.0 41.0 33.0 41.0 26-27 37.84873595124358 40.0 38.0 41.0 33.0 41.0 28-29 37.718301335079936 40.0 38.0 41.0 32.0 41.0 30-31 37.52709938727341 40.0 37.5 41.0 32.0 41.0 32-33 37.300394725943534 40.0 37.0 41.0 31.5 41.0 34-35 36.97229122714456 40.0 36.5 41.0 30.5 41.0 36-37 36.712668955719835 40.0 36.0 41.0 30.0 41.0 38-39 36.46431666333726 39.5 35.5 41.0 30.0 41.0 40-41 36.30343886968197 39.0 35.0 41.0 30.0 41.0 42-43 36.08517129268397 39.0 35.0 41.0 29.5 41.0 44-45 35.79110881726298 39.0 35.0 41.0 27.5 41.0 46-47 35.372842701955115 39.0 35.0 41.0 25.5 41.0 48-49 35.29359334248862 39.0 35.0 41.0 24.5 41.0 50-51 34.292913308549565 38.0 34.0 40.0 23.5 40.5 52-53 34.46168283690392 38.5 34.5 39.5 24.0 40.5 54-55 34.96173921638095 39.0 35.0 40.5 24.0 41.0 56-57 34.85715006615888 39.0 35.0 41.0 23.5 41.0 58-59 34.67226888416663 39.0 35.0 41.0 23.0 41.0 60-61 34.39085838101808 38.0 34.0 40.5 22.5 41.0 62-63 34.02953066521519 38.0 34.0 40.0 22.0 41.0 64-65 33.663493174951086 37.0 34.0 40.0 20.5 41.0 66-67 33.273201024853606 37.0 33.5 40.0 18.5 41.0 68-69 32.875118762324306 36.0 33.0 39.0 17.5 41.0 70-71 32.468421228474 36.0 32.5 39.0 14.0 41.0 72-73 32.01242819447161 35.0 32.0 38.5 10.0 40.0 74-75 31.554959897486796 35.0 31.5 37.5 7.5 39.5 76-77 31.102606471946338 35.0 31.0 37.0 6.0 39.0 78-79 30.672158909384258 35.0 31.0 36.5 2.0 39.0 80-81 30.244246683181444 35.0 31.0 36.0 2.0 37.5 82-83 29.811276313032778 34.5 30.0 35.5 2.0 37.0 84-85 29.429096543242018 34.0 30.0 35.0 2.0 37.0 86-87 29.032479451072707 34.0 29.0 35.0 2.0 36.0 88-89 28.618613719145287 34.0 29.0 35.0 2.0 36.0 90-91 28.226686564213786 34.0 29.0 35.0 2.0 36.0 92-93 27.803188642249946 34.0 27.5 35.0 2.0 35.0 94-95 27.33491601894072 34.0 26.5 35.0 2.0 35.0 96-97 26.765778771108373 34.0 25.5 35.0 2.0 35.0 98-99 26.04544951496249 33.0 24.5 35.0 2.0 35.0 100-101 23.520285475045817 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4600595582583864 1101 2 0.43123349272494593 1101 3 0.5455100846047358 1101 4 0.4329755583566879 1101 5 0.49590506269417745 1101 6 0.49061146586029736 1101 7 0.39194044426923824 1101 8 0.49749494583898723 1101 9 0.5530356588367766 1101 10-11 0.6618504150356515 1101 12-13 0.530292438332232 1101 14-15 0.670545335270269 1101 16-17 0.7755486712858328 1101 18-19 0.8151351594731651 1101 20-21 0.7911621946143299 1101 22-23 0.7741722132022986 1101 24-25 0.7143639342835044 1101 26-27 0.6930887196686726 1101 28-29 0.6906149191193194 1101 30-31 0.6514004026473401 1101 32-33 0.7358773018591407 1101 34-35 0.6675445322152456 1101 36-37 0.5742129349922109 1101 38-39 0.6262675961850164 1101 40-41 0.3717832809341388 1101 42-43 0.5219538859006079 1101 44-45 0.6192720696701812 1101 46-47 0.6194618486763446 1101 48-49 0.7876313220255327 1101 50-51 0.6478957971488839 1101 52-53 0.7496700278546413 1101 54-55 0.6708866419134054 1101 56-57 0.61897206927393 1101 58-59 0.738162190722214 1101 60-61 0.6978828102406354 1101 62-63 0.7154147146763421 1101 64-65 0.6175540076081916 1101 66-67 0.4227251306743085 1101 68-69 0.5775999494237354 1101 70-71 0.5652737245523056 1101 72-73 0.5068717363367767 1101 74-75 0.3067499817413726 1101 76-77 0.28579820047245263 1101 78-79 0.23098095927660012 1101 80-81 0.12543569430802037 1101 82-83 -0.028821857875815482 1101 84-85 -0.11505946339878292 1101 86-87 0.05667487810395855 1101 88-89 0.02721030158668114 1101 90-91 -0.061230059313817975 1101 92-93 -0.18876202705188305 1101 94-95 -0.26132708948238914 1101 96-97 -0.19596632115920087 1101 98-99 -0.28804804415993246 1101 100-101 -0.45133186760415356 1102 1 0.21216522753834255 1102 2 0.350515198987285 1102 3 0.36594597504699067 1102 4 0.349016876798224 1102 5 0.36710314363859453 1102 6 0.44942706914358155 1102 7 0.404837887160312 1102 8 0.4757820162253381 1102 9 0.4995669285792488 1102 10-11 0.5052073868815796 1102 12-13 0.5113734690086815 1102 14-15 0.5775153162242432 1102 16-17 0.6663167131357923 1102 18-19 0.7131981535448872 1102 20-21 0.7156384290505571 1102 22-23 0.709359211554137 1102 24-25 0.5807474749783452 1102 26-27 0.6069643400630653 1102 28-29 0.7373930644518722 1102 30-31 0.7190802150312194 1102 32-33 0.7691466433610472 1102 34-35 0.5772461134778837 1102 36-37 0.6911740340198591 1102 38-39 0.613834064931595 1102 40-41 0.5982724023227988 1102 42-43 0.6426315232615778 1102 44-45 0.5268032904571598 1102 46-47 0.49350051419605023 1102 48-49 0.6466995123738357 1102 50-51 0.5164421218730411 1102 52-53 0.5372853355399556 1102 54-55 0.6175280615296472 1102 56-57 0.5954495345912889 1102 58-59 0.5645012984732674 1102 60-61 0.6558469375156051 1102 62-63 0.5071807493508231 1102 64-65 0.4849522093095118 1102 66-67 0.4361424343452711 1102 68-69 0.4666149181504977 1102 70-71 0.502477223238671 1102 72-73 0.4795854562320585 1102 74-75 0.4576442355313439 1102 76-77 0.3300446471191165 1102 78-79 0.4228766094639127 1102 80-81 0.13135115049168178 1102 82-83 0.2319990559135512 1102 84-85 0.2533941955585597 1102 86-87 0.13213162125776634 1102 88-89 0.1905882077366563 1102 90-91 0.10233317836979339 1102 92-93 0.0793130427097033 1102 94-95 0.0012288305196612725 1102 96-97 -0.04384643147225731 1102 98-99 -0.1408277840430472 1102 100-101 0.15589349787196483 1103 1 0.4078352327919532 1103 2 0.388763203461334 1103 3 0.5251406468655873 1103 4 0.3898459309782396 1103 5 0.5033852796563565 1103 6 0.358307371480592 1103 7 0.5025720542930259 1103 8 0.46003134257644973 1103 9 0.6074626019739284 1103 10-11 0.5732840557513725 1103 12-13 0.45755019380404605 1103 14-15 0.6108503442717605 1103 16-17 0.6428940683648321 1103 18-19 0.6811485662077885 1103 20-21 0.7268498107510339 1103 22-23 0.6432704424250488 1103 24-25 0.589441358754847 1103 26-27 0.6516505637314438 1103 28-29 0.8112876871237589 1103 30-31 0.7667440515251869 1103 32-33 0.7499040111309725 1103 34-35 0.6158084874335117 1103 36-37 0.5891522399954212 1103 38-39 0.6326589501147097 1103 40-41 0.583190293420472 1103 42-43 0.5256233716863363 1103 44-45 0.5238095778467908 1103 46-47 0.5243070286773914 1103 48-49 0.464166931493601 1103 50-51 0.4051146522507949 1103 52-53 0.4398384224618468 1103 54-55 0.37279692793531893 1103 56-57 0.39417510952307566 1103 58-59 0.29909973594678974 1103 60-61 0.35282610947825077 1103 62-63 0.31388503867243145 1103 64-65 0.293887589857583 1103 66-67 0.24239847176455953 1103 68-69 0.02286417671304264 1103 70-71 0.19765915080658658 1103 72-73 0.1457072722877797 1103 74-75 0.14127753327568016 1103 76-77 0.07263111991048277 1103 78-79 0.20214432346394773 1103 80-81 0.2667418668473651 1103 82-83 0.3056301505294421 1103 84-85 0.25834530305427705 1103 86-87 0.2776084290941263 1103 88-89 0.5335703962905569 1103 90-91 0.39047356849299675 1103 92-93 0.12476019304780195 1103 94-95 -0.03308235289925676 1103 96-97 0.19675722572095466 1103 98-99 0.2625904544710309 1103 100-101 -0.018082471080873574 1104 1 0.09133889620749613 1104 2 0.03360596394697879 1104 3 0.15921443713331485 1104 4 0.14579276911329941 1104 5 0.17762476347430578 1104 6 0.21692313069188174 1104 7 0.16111229633665403 1104 8 0.27261716700459004 1104 9 0.36522351795274943 1104 10-11 0.3235332346409976 1104 12-13 0.26183523779557305 1104 14-15 0.4048814340233591 1104 16-17 0.5002107657103494 1104 18-19 0.4985055763942796 1104 20-21 0.4167541093992213 1104 22-23 0.5065130163388645 1104 24-25 0.4865991924116244 1104 26-27 0.4174898731935812 1104 28-29 0.44338387156333425 1104 30-31 0.28575660903067757 1104 32-33 0.3542018264082074 1104 34-35 0.1826375715379882 1104 36-37 0.31722796816693943 1104 38-39 0.3857241975028387 1104 40-41 0.091386960114896 1104 42-43 0.23296713860482043 1104 44-45 0.29503309478123896 1104 46-47 0.573470370645218 1104 48-49 0.5509361571372366 1104 50-51 0.45863716719458836 1104 52-53 0.4629827685303738 1104 54-55 0.6165421907600503 1104 56-57 0.44849860619005 1104 58-59 0.6116428043314741 1104 60-61 0.5073209079556946 1104 62-63 0.5836040426232003 1104 64-65 0.5631468275228784 1104 66-67 0.5395599146977332 1104 68-69 0.4990779347193808 1104 70-71 0.5530135123394757 1104 72-73 0.5076943732892119 1104 74-75 0.46847204273337084 1104 76-77 0.4889786432527785 1104 78-79 0.4287999401921141 1104 80-81 0.3788737553595958 1104 82-83 0.2521656616446286 1104 84-85 0.3495167353539692 1104 86-87 0.17692738640193895 1104 88-89 0.40743756936646847 1104 90-91 0.3573245858924885 1104 92-93 0.2533065153339713 1104 94-95 0.007198282222336871 1104 96-97 0.17382388350324973 1104 98-99 0.10375314121638013 1104 100-101 -0.06166948101748915 1105 1 0.24766839344977853 1105 2 0.2662851359278058 1105 3 0.26574080802939903 1105 4 0.32058350971961147 1105 5 0.3811112101631693 1105 6 0.2805707324152351 1105 7 0.16599135058010006 1105 8 0.28405920624579295 1105 9 0.39568104585974595 1105 10-11 0.34580080795264934 1105 12-13 0.26454261482172825 1105 14-15 0.40327698395286404 1105 16-17 0.45726629250567186 1105 18-19 0.4146998187075681 1105 20-21 0.35181547643519195 1105 22-23 0.38022085912182746 1105 24-25 0.35401905336282624 1105 26-27 0.3766328276813553 1105 28-29 0.255413908903364 1105 30-31 0.2832593259453873 1105 32-33 0.29017033298205774 1105 34-35 0.41733793730809055 1105 36-37 0.3966752700891547 1105 38-39 0.33467269454961013 1105 40-41 0.4417457624151737 1105 42-43 0.22633977425317653 1105 44-45 0.31214581587662593 1105 46-47 0.3470664635867493 1105 48-49 0.5241744810273801 1105 50-51 0.32964849531150975 1105 52-53 0.2894345759476167 1105 54-55 0.44825853373722424 1105 56-57 0.3094754979760381 1105 58-59 0.44424005822086343 1105 60-61 0.5033654192581096 1105 62-63 0.46110549844439674 1105 64-65 0.375413452365045 1105 66-67 0.3550672319544219 1105 68-69 0.24457310202888038 1105 70-71 0.0879314962218487 1105 72-73 0.3878454501729962 1105 74-75 0.3441094115836201 1105 76-77 0.38391365719634507 1105 78-79 0.3352771390384781 1105 80-81 0.13603721692353332 1105 82-83 0.2186338208878844 1105 84-85 0.2613768959791365 1105 86-87 0.17108343665704595 1105 88-89 0.14570131912736173 1105 90-91 0.1509172240736305 1105 92-93 0.06355194638686257 1105 94-95 0.14575298427309846 1105 96-97 0.1465696920893862 1105 98-99 0.20625540509118778 1105 100-101 0.13748129436957157 1106 1 0.048023456319363333 1106 2 0.1489691959706967 1106 3 0.013913203896571247 1106 4 0.07254564582331824 1106 5 0.16009599637409622 1106 6 0.19241370872948238 1106 7 0.27581005225012944 1106 8 0.2653790613370859 1106 9 0.2685331033358622 1106 10-11 0.1333128904466392 1106 12-13 0.17922116971482893 1106 14-15 0.13095483014169673 1106 16-17 0.23717554180712597 1106 18-19 0.184160249047423 1106 20-21 0.2177541136110932 1106 22-23 0.22520220740631913 1106 24-25 0.3416944018260111 1106 26-27 0.4034290579432209 1106 28-29 0.2926917027008855 1106 30-31 0.2357121737587633 1106 32-33 0.2021887085262719 1106 34-35 0.3824799421492102 1106 36-37 0.39237938504138725 1106 38-39 0.5506838266894505 1106 40-41 0.5062160212039188 1106 42-43 0.4547067795004338 1106 44-45 0.37353453103030887 1106 46-47 0.3683339375129364 1106 48-49 0.4435080959471307 1106 50-51 0.3421193551789514 1106 52-53 0.24727479461029844 1106 54-55 0.08590580817025995 1106 56-57 0.28701272018021484 1106 58-59 0.2253276045201602 1106 60-61 0.22058099328825875 1106 62-63 0.11619420831832628 1106 64-65 0.03692949060664574 1106 66-67 -0.062133416641977135 1106 68-69 0.16722305694797512 1106 70-71 0.2326749513683808 1106 72-73 0.29736127722478756 1106 74-75 0.14359255498149892 1106 76-77 0.23928874248453624 1106 78-79 0.17590980482248142 1106 80-81 0.31871081900943565 1106 82-83 0.39506691636192315 1106 84-85 0.36900323419149217 1106 86-87 0.3261693543579973 1106 88-89 0.22012262858541476 1106 90-91 0.41812614765541056 1106 92-93 0.4430361761938677 1106 94-95 0.30299293651171055 1106 96-97 0.2580697895616062 1106 98-99 0.48746250716084916 1106 100-101 0.4037510324942062 1107 1 0.12614494282755828 1107 2 0.16531195215444328 1107 3 0.011624414947007722 1107 4 0.14399952873369415 1107 5 0.10085752062722975 1107 6 0.1947441576533535 1107 7 0.1890769973191908 1107 8 0.21622832249995128 1107 9 0.3328315092144365 1107 10-11 0.40583788643122176 1107 12-13 0.2265275763161796 1107 14-15 0.32154369136880945 1107 16-17 0.2536774089770546 1107 18-19 0.3110409710197999 1107 20-21 0.23500084104443886 1107 22-23 0.34635669062186736 1107 24-25 0.3097984212606377 1107 26-27 0.23632387403702637 1107 28-29 0.22182883930544506 1107 30-31 0.28839146222647827 1107 32-33 0.08093986295930478 1107 34-35 0.2174336644428223 1107 36-37 0.17450976570312804 1107 38-39 -0.00283819788491968 1107 40-41 0.03223543575579413 1107 42-43 -0.0031732297350330896 1107 44-45 0.3076279497582206 1107 46-47 0.29289399814576456 1107 48-49 0.24009498961883224 1107 50-51 0.34727264380727974 1107 52-53 0.37940115985282574 1107 54-55 0.42065097962865394 1107 56-57 0.5077839818249288 1107 58-59 0.38615919783850217 1107 60-61 0.19664633419735367 1107 62-63 0.23620078356728413 1107 64-65 0.26019031216141997 1107 66-67 0.24975147132782638 1107 68-69 0.33997617893641063 1107 70-71 0.3531282420044235 1107 72-73 0.2355935893031642 1107 74-75 0.2912437857074899 1107 76-77 0.38763489169751253 1107 78-79 0.3782510451854719 1107 80-81 0.32879504242736246 1107 82-83 0.23945712476218262 1107 84-85 0.24230395897466295 1107 86-87 0.2991380541652582 1107 88-89 0.3048346728353373 1107 90-91 0.06985578948001248 1107 92-93 0.07340830516913499 1107 94-95 -0.08724256782494777 1107 96-97 -0.07436676681487953 1107 98-99 0.024593339557249294 1107 100-101 0.25774235918871113 1108 1 -0.1682728974219181 1108 2 0.04608391608126894 1108 3 0.09592457538617793 1108 4 -0.08964385675254505 1108 5 -0.002908770541779404 1108 6 0.09098275047730198 1108 7 0.22680584291419592 1108 8 0.21568180368803525 1108 9 0.13804712529351804 1108 10-11 0.1667112420518393 1108 12-13 0.3009117658541882 1108 14-15 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-2.022518398184257 2316 98-99 -2.0791226679078605 2316 100-101 -1.8657496949940757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5882.0 3 1743.0 4 1587.0 5 1768.0 6 2053.0 7 2378.0 8 2964.0 9 3442.0 10 4153.0 11 5159.0 12 6527.0 13 9205.0 14 8434.0 15 8896.0 16 11029.0 17 15015.0 18 14251.0 19 9123.0 20 8854.0 21 9371.0 22 9923.0 23 10669.0 24 11893.0 25 13385.0 26 15335.0 27 17397.0 28 20389.0 29 24328.0 30 28441.0 31 35115.0 32 43710.0 33 56882.0 34 77990.0 35 115413.0 36 181993.0 37 292833.0 38 296533.0 39 52614.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18561029081555 19.41359169325905 15.700924919035536 21.69987309688986 2 15.16487866340891 20.994763522224716 40.150627136857864 23.689730677508514 3 18.915685234724886 25.527102294463727 30.482703546812928 25.074508923998458 4 12.750246178055205 17.635965899548204 37.79712976946026 31.816658152936334 5 13.62567690301319 37.70240872569718 33.684589857241335 14.987324514048286 6 30.167035775068427 36.51066118356658 18.162571641746332 15.159731399618654 7 27.41816707342477 33.88725821812778 20.816145103610104 17.878429604837347 8 26.213506999869708 34.42390511419337 20.244126247786955 19.11846163814997 9 26.73966262186312 17.134264348742043 20.82474700819689 35.30132602119795 10-11 24.788844349513806 27.290004277451974 26.885178977229184 21.03597239580503 12-13 26.088069698883466 24.739118606328873 27.58618047067159 21.586631224116076 14-15 23.09953590250295 25.736458249513277 26.69440174338749 24.469604104596282 16-17 21.63054957241856 29.071497635693365 27.236826339796977 22.061126452091095 18-19 21.53708310443173 27.392354123346642 30.06183739736006 21.00872537486157 20-21 22.847633666933074 26.249675121746602 29.920189694060497 20.982501517259834 22-23 22.291132995314378 26.316733889512957 29.855584699392622 21.53654841578004 24-25 21.965684726859877 26.56056921703386 30.03203204877569 21.441714007330575 26-27 21.78701683199438 26.651575416409084 29.976958894696015 21.58444885690052 28-29 21.518970580651274 26.870784567072686 30.10312173759117 21.507123114684866 30-31 21.770909150456827 26.657291910196058 29.969558753487224 21.602240185859888 32-33 21.58462215954224 26.748660075977803 29.82985821750388 21.83685954697608 34-35 22.03300584479229 26.706989064278048 29.730156285953818 21.529848804975842 36-37 22.02800492291249 26.491851278799615 29.779493670266287 21.700650128021607 38-39 21.896904203054575 26.702188326802535 29.787705692125176 21.613201778017714 40-41 22.012936983244284 26.65026238920296 29.712448560390065 21.624352067162686 42-43 21.84563372322558 26.716530072081518 29.425120791529398 22.012715413163505 44-45 21.899592479350318 26.915107500051583 29.290918266108573 21.89438175448953 46-47 22.07434930456439 27.04880622336967 28.802809931287065 22.07403454077887 48-49 22.198461075148767 27.157102338607537 28.581057282435978 22.063379303807718 50-51 22.094530366374592 27.385519743546876 28.473333114166543 22.046616775911986 52-53 22.243448139821513 27.449016993635954 28.25414068609689 22.053394180445636 54-55 21.908462456304285 27.27836707059997 28.428723700389014 22.384446772706735 56-57 22.13402708483818 27.620989875526096 28.14021966311423 22.104763376521486 58-59 22.236212083185062 27.225162734086457 28.304825010608916 22.233800172119565 60-61 22.14956683373718 27.190332854075645 28.68614095067722 21.97395936150996 62-63 22.070943868069175 27.255034488690775 28.598357368362713 22.07566427487733 64-65 21.88785929965314 27.213439060057667 28.588746896279872 22.309954744009325 66-67 21.860002574485176 27.37080002126749 28.314544721885643 22.454652682361697 68-69 21.9106202163827 27.3063691072167 28.46640176822856 22.31660890817204 70-71 21.955243182811166 27.212016120653736 28.34274264497797 22.489998051557127 72-73 21.95028447944773 27.162173222904144 28.35563673656986 22.531905561078258 74-75 21.836006163630035 27.510253768751937 28.144142190257476 22.509597877360555 76-77 21.89093353393914 27.2493397949666 28.184940645372937 22.674786025721332 78-79 21.86047389838521 27.3131095848931 28.218688656886968 22.60772785983472 80-81 21.842044163967262 27.53922531231992 28.054630111388978 22.56410041232384 82-83 21.913267233106815 27.447389330147693 28.075402098831404 22.563941337914095 84-85 21.827041574984225 27.33970319369626 27.9925777441434 22.840677487176116 86-87 21.88132336679217 27.24091550985265 28.081991051375905 22.79577007197928 88-89 21.892630172537416 27.113557110828967 28.040880140184466 22.95293257644915 90-91 21.754756397716985 27.1294823197872 28.26109009905285 22.854671183442964 92-93 21.934408568300505 27.094626973217068 28.178501596762402 22.792462861720026 94-95 21.687040735194476 27.028163402513165 28.20436402788461 23.08043183440774 96-97 21.66527563137028 26.841011311810416 28.267947678416895 23.22576537840241 98-99 21.615555255519745 26.79636142064935 28.064778353209153 23.52330497062175 100-101 21.80760950005386 26.227516972875577 28.690699857446035 23.274173669624535 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1795.0 1 1046.0 2 379.0 3 465.0 4 400.0 5 411.5 6 758.5 7 1530.5 8 2497.0 9 3035.5 10 2737.5 11 2292.0 12 2269.0 13 2411.5 14 2570.5 15 2709.5 16 2779.0 17 2819.5 18 2949.0 19 3034.0 20 3161.5 21 3508.5 22 4000.0 23 4707.0 24 5594.0 25 6772.0 26 8410.0 27 10347.5 28 12147.5 29 14168.5 30 16591.0 31 19526.0 32 22756.0 33 25610.0 34 28585.0 35 31361.5 36 34598.5 37 38102.0 38 39939.0 39 40783.0 40 42259.5 41 44794.0 42 47107.5 43 49115.5 44 51322.5 45 52827.0 46 53343.5 47 55307.5 48 61511.5 49 61522.5 50 55467.5 51 51547.0 52 47981.0 53 46328.5 54 44689.5 55 41012.0 56 36974.5 57 33033.5 58 29129.0 59 25318.0 60 21341.5 61 17489.5 62 14025.0 63 10855.5 64 8270.5 65 6296.5 66 4754.0 67 3751.5 68 2817.5 69 1986.5 70 1386.5 71 1026.0 72 813.5 73 679.0 74 559.5 75 436.0 76 329.0 77 205.5 78 111.0 79 68.5 80 50.0 81 36.5 82 32.0 83 27.0 84 17.5 85 13.0 86 8.0 87 7.0 88 7.0 89 7.5 90 5.0 91 4.0 92 4.5 93 3.0 94 3.5 95 3.0 96 2.5 97 3.5 98 2.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34050419957499617 2 0.12243395074661044 3 0.11345499699657478 4 0.12222513786870264 5 0.14157513122149268 6 0.08714457438019117 7 0.0946618379848722 8 0.10106543290737827 9 0.08846705594027394 10-11 0.08763180442864273 12-13 0.09177325984048089 14-15 0.09233009418156836 16-17 0.08192425243249601 18-19 0.09720239466608385 20-21 0.10719061065934059 22-23 0.11589114723883252 24-25 0.1308560701555586 26-27 0.12988161005865553 28-29 0.12438287094041665 30-31 0.11369861202080055 32-33 0.10698179778143278 34-35 0.3295415234848364 36-37 0.4908146695222988 38-39 0.4645042469059152 40-41 0.4437273655540885 42-43 0.4770330195803836 44-45 0.4837150316734333 46-47 0.49067546093702685 48-49 0.5003504576134219 50-51 0.48973580298644176 52-53 0.4941208734225057 54-55 0.4815921007480373 56-57 0.45897070564135833 58-59 0.4379502092653058 60-61 0.3939950984657126 62-63 0.46854129587879945 64-65 0.4982623288343439 66-67 0.5067888546822459 68-69 0.5196656488198941 70-71 0.5113479358498998 72-73 0.5039350786841726 74-75 0.5109303100940842 76-77 0.48886574932849264 78-79 0.4680192636840299 80-81 0.4697593709999283 82-83 0.48340181235657165 84-85 0.48298418660075604 86-87 0.48555954542828567 88-89 0.4975314837616666 90-91 0.5119395723373052 92-93 0.5037958700989008 94-95 0.5082853469739186 96-97 0.5082505448276006 98-99 0.503517452928357 100-101 0.49133670171706834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1436693.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.800726038652954 #Duplication Level Percentage of deduplicated Percentage of total 1 79.00983062857016 48.828648970366345 2 12.53614625541179 15.494858806223782 3 3.896486613993423 7.224171052340581 4 1.6287503093080227 4.026318066036655 5 0.8245603990349734 2.5479215661541375 6 0.4791410623528521 1.7766759317002647 7 0.313053248960993 1.3542842652183982 8 0.21443393909782218 1.0601738498858961 9 0.15299451620105808 0.8509654963042056 >10 0.8627231319083204 9.630558561689494 >50 0.05459939032519379 2.310392719623096 >100 0.025702340738242953 2.9651572904073205 >500 7.890820485934796E-4 0.3419878939665332 >1k 5.636300347096284E-4 0.6180722337076965 >5k 2.2545201388385133E-4 0.9698132963756585 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7552 0.5256516179865844 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6369 0.4433097397982729 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3676 0.2558653797296987 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1571 0.10934834373105458 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1528 0.10635535914770936 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.3920858527187088E-4 0.0 22-23 0.0 0.0 0.0 1.7401073158983862E-4 0.0 24-25 0.0 0.0 0.0 2.7841717054374177E-4 0.0 26-27 0.0 0.0 0.0 7.656472189952899E-4 0.0 28-29 0.0 0.0 0.0 0.0028189738517553856 0.0 30-31 0.0 0.0 0.0 0.007378055019409157 0.0 32-33 0.0 0.0 0.0 0.01708785384212215 0.0 34-35 0.0 0.0 0.0 0.03097391022299127 6.960429263593544E-5 36-37 0.0 0.0 0.0 0.053490898890716385 6.960429263593544E-5 38-39 0.0 0.0 0.0 0.08853666023290988 6.960429263593544E-5 40-41 0.0 0.0 0.0 0.13103008088714846 6.960429263593544E-5 42-43 0.0 0.0 0.0 0.17568123461310103 6.960429263593544E-5 44-45 0.0 0.0 0.0 0.23007698930808462 6.960429263593544E-5 46-47 0.0 0.0 0.0 0.2988112282860709 6.960429263593544E-5 48-49 0.0 0.0 0.0 0.3723133613096187 6.960429263593544E-5 50-51 0.0 0.0 0.0 0.45409840515684285 6.960429263593544E-5 52-53 0.0 0.0 0.0 0.5414517924149418 6.960429263593544E-5 54-55 0.0 0.0 0.0 0.6379233420083483 6.960429263593544E-5 56-57 0.0 0.0 0.0 0.753710082808227 6.960429263593544E-5 58-59 0.0 0.0 0.0 0.8917353951052869 6.960429263593544E-5 60-61 0.0 0.0 0.0 1.0388440675913364 6.960429263593544E-5 62-63 0.0 0.0 0.0 1.1903726126597678 6.960429263593544E-5 64-65 0.0 0.0 0.0 1.3522373951846358 6.960429263593544E-5 66-67 0.0 0.0 0.0 1.5418046861786059 6.960429263593544E-5 68-69 0.0 0.0 0.0 1.762867919590337 6.960429263593544E-5 70-71 0.0 0.0 0.0 2.005682494450798 6.960429263593544E-5 72-73 0.0 0.0 0.0 2.261965499936312 6.960429263593544E-5 74-75 0.0 0.0 0.0 2.5313341124373823 6.960429263593544E-5 76-77 0.0 0.0 0.0 2.817477359463713 6.960429263593544E-5 78-79 0.0 0.0 0.0 3.1537356971879174 6.960429263593544E-5 80-81 0.0 0.0 0.0 3.52392612757214 6.960429263593544E-5 82-83 0.0 0.0 0.0 3.911865652578526 6.960429263593544E-5 84-85 0.0 0.0 0.0 4.307183232604321 6.960429263593544E-5 86-87 0.0 0.0 0.0 4.7310037704645325 6.960429263593544E-5 88-89 0.0 0.0 0.0 5.1872947108394065 6.960429263593544E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7810 0.0 27.663403 1 GTATCAA 10860 0.0 27.196047 1 GAGTACT 6180 0.0 23.37372 12-13 GTACACG 190 3.2989556E-7 22.492315 1 ATCAACG 12905 0.0 22.470575 3 TCAACGC 13045 0.0 22.411034 4 TATCAAC 12980 0.0 22.378021 2 CAACGCA 13120 0.0 22.319036 5 GTACATG 8010 0.0 21.93383 1 AACGCAG 13380 0.0 21.91158 6 TACATGG 8035 0.0 21.819849 2 GTACTTT 6610 0.0 21.711782 14-15 GTCGGGA 310 1.0913936E-11 21.399435 2 ACATGGG 8085 0.0 20.863674 3 AGTACTT 6425 0.0 20.27104 12-13 ACGCAGA 14685 0.0 19.835371 7 CGCAGAG 14780 0.0 19.611742 8 CATGGGG 3145 0.0 19.435238 4 GTAGCCC 750 0.0 18.953123 3 GCAGAGT 15195 0.0 18.544928 9 >>END_MODULE