FastQCFastQC Report
Fri 27 May 2016
SRR939129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939129_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436693
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70700.49210234893606364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64130.44637232867425397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37280.25948480294676735No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16150.11241093260703575No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15980.11122765963222485No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71500.031.7651371
GTATCAA104350.029.7960781
ATCAACG123850.025.0574173
TCAACGC125650.024.6984584
TATCAAC126150.024.6757972
CAACGCA127200.024.43485
AACGCAG129650.023.9730556
GAGTACT64150.023.41407412-13
GTACTTT67350.022.301614-15
ACGCAGA141850.021.8777687
CGCAGAG142950.021.709428
TACCTGG11550.020.5419922
GCAGAGT151250.020.3940169
AGTACTT66550.020.10948612-13
GTACATG83350.019.4227921
ACTTTTT79100.018.77716316-17
TACATGG83150.018.7753582
ACCGTCC1559.691692E-418.3685198
AGAGTAC127400.018.27130310-11
GACCGGG1300.007608246518.2507726
Produced by FastQC (version 0.11.5)