##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939129_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1436693 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.504192614566925 34.0 31.0 34.0 31.0 34.0 2 32.71859541321632 34.0 31.0 34.0 31.0 34.0 3 32.79599886684212 34.0 31.0 34.0 31.0 34.0 4 36.25610273036759 37.0 35.0 37.0 35.0 37.0 5 36.223710284660676 37.0 37.0 37.0 35.0 37.0 6 36.264661274190104 37.0 37.0 37.0 35.0 37.0 7 36.2170644667998 37.0 37.0 37.0 35.0 37.0 8 36.205255402511185 37.0 37.0 37.0 35.0 37.0 9 37.946921854564614 39.0 38.0 39.0 35.0 39.0 10-11 37.89481712516174 39.0 38.0 39.0 35.0 39.0 12-13 37.83272591987293 39.0 38.0 39.0 35.0 39.0 14-15 39.18680782881242 40.5 39.0 41.0 36.0 41.0 16-17 39.129960262909336 40.0 38.5 41.0 36.0 41.0 18-19 39.18218993201749 40.5 38.5 41.0 36.0 41.0 20-21 39.25546411098266 41.0 39.0 41.0 36.0 41.0 22-23 39.22233142362356 41.0 39.0 41.0 36.0 41.0 24-25 39.173391253385375 41.0 39.0 41.0 36.0 41.0 26-27 39.040179773967026 40.5 39.0 41.0 35.0 41.0 28-29 38.89691360645594 40.0 38.0 41.0 35.0 41.0 30-31 38.69937418780491 40.0 38.0 41.0 35.0 41.0 32-33 38.54382286264359 40.0 38.0 41.0 34.0 41.0 34-35 38.487037244560945 40.0 38.0 41.0 34.0 41.0 36-37 38.38293288823708 40.0 38.0 41.0 34.0 41.0 38-39 38.25183459514315 40.0 38.0 41.0 34.0 41.0 40-41 38.08713587384361 40.0 38.0 41.0 33.5 41.0 42-43 37.92268111558976 40.0 38.0 41.0 33.0 41.0 44-45 37.67399889886009 40.0 37.0 41.0 33.0 41.0 46-47 37.34177900219463 40.0 37.0 41.0 32.5 41.0 48-49 36.9982532802763 40.0 37.0 41.0 31.5 41.0 50-51 36.747112639930734 40.0 36.5 41.0 31.0 41.0 52-53 36.52057607296757 40.0 36.0 41.0 30.5 41.0 54-55 36.2908968721919 39.0 35.5 41.0 30.0 41.0 56-57 36.03934556651978 39.0 35.0 41.0 29.5 41.0 58-59 35.75334674840067 39.0 35.0 40.0 29.0 41.0 60-61 35.417124604908636 38.0 35.0 40.0 28.0 41.0 62-63 35.04776142154239 38.0 34.5 40.0 28.0 41.0 64-65 34.65408163052231 37.0 34.0 40.0 27.0 41.0 66-67 34.30397482273527 37.0 34.0 39.5 26.0 41.0 68-69 33.858595051274 36.0 34.0 39.0 26.0 40.5 70-71 33.43785867962049 36.0 33.0 39.0 26.0 40.0 72-73 32.98571023872184 35.0 33.0 38.0 25.0 40.0 74-75 32.49864584848677 35.0 32.5 37.0 23.5 39.5 76-77 30.522945751110363 33.5 29.5 35.5 20.0 38.0 78-79 31.47528699589961 34.5 31.5 36.0 21.0 39.0 80-81 31.497693661763506 35.0 32.0 36.0 21.0 37.5 82-83 31.20896113505112 35.0 32.0 35.5 20.0 37.0 84-85 30.83821247823996 34.5 31.0 35.0 19.0 37.0 86-87 30.300403774501582 34.0 31.0 35.0 14.0 36.0 88-89 29.767256470241033 34.0 30.0 35.0 7.0 36.0 90-91 29.332325347168812 34.0 30.0 35.0 2.0 35.5 92-93 29.070610422685988 34.0 30.0 35.0 2.0 35.0 94-95 28.760636057946968 34.0 29.0 35.0 2.0 35.0 96-97 28.248122598216877 34.0 29.0 35.0 2.0 35.0 98-99 27.536857561079508 34.0 29.0 35.0 2.0 35.0 100-101 24.01882274083607 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2440408615519445 1101 2 0.20609733092761218 1101 3 0.25434774571525764 1101 4 0.18064691869595606 1101 5 0.2616813306551933 1101 6 0.2502243015840975 1101 7 0.20280105806741489 1101 8 0.15752615649389412 1101 9 0.28570431543733577 1101 10-11 0.23316797007696266 1101 12-13 0.2955329566827416 1101 14-15 0.4004569969762457 1101 16-17 0.4254857888259025 1101 18-19 0.38029347908983624 1101 20-21 0.4136854277591553 1101 22-23 0.38866969042803845 1101 24-25 0.39949134353577875 1101 26-27 0.3495741135804664 1101 28-29 0.33131687291265877 1101 30-31 0.3616442742005219 1101 32-33 0.5768094133691832 1101 34-35 0.5794789530879498 1101 36-37 0.5335950448180427 1101 38-39 0.39776174604892134 1101 40-41 0.3917847713027669 1101 42-43 0.4915528016603403 1101 44-45 0.5700853595842048 1101 46-47 0.5631800577443116 1101 48-49 0.47921779108592943 1101 50-51 0.5592228923276892 1101 52-53 0.3262084237701899 1101 54-55 0.4567680978005271 1101 56-57 0.48957176499549604 1101 58-59 0.6498279974636887 1101 60-61 0.6131851735545979 1101 62-63 0.4592417552029815 1101 64-65 0.4137148730685638 1101 66-67 0.45948748163489483 1101 68-69 0.32995922612756345 1101 70-71 0.37144390392091964 1101 72-73 0.5251939290661269 1101 74-75 0.4896997854524656 1101 76-77 0.390692715510248 1101 78-79 0.5746061794399786 1101 80-81 0.5351191435752121 1101 82-83 0.6139883644830135 1101 84-85 0.6843481818138422 1101 86-87 0.7219009132876408 1101 88-89 0.8754791384153577 1101 90-91 0.6752581071882453 1101 92-93 0.6697182673200395 1101 94-95 0.5875657293846821 1101 96-97 0.7203963619804519 1101 98-99 0.9805736990190432 1101 100-101 0.5898604011713822 1102 1 0.0803628115660473 1102 2 0.12860893454659106 1102 3 0.17080285468787082 1102 4 0.12184437021252137 1102 5 0.11635459932064407 1102 6 0.13350739725013483 1102 7 0.15233143495482437 1102 8 0.13122889403879867 1102 9 0.20007648446902238 1102 10-11 0.19823006196876491 1102 12-13 0.19715338371457136 1102 14-15 0.2641992719149897 1102 16-17 0.19722319618927742 1102 18-19 0.2793046945616382 1102 20-21 0.14614245765231715 1102 22-23 0.29527192233256727 1102 24-25 0.24514864105233158 1102 26-27 0.14727541975982206 1102 28-29 0.07925957632858882 1102 30-31 0.22206864844433483 1102 32-33 0.1714782760396858 1102 34-35 0.1859460911261337 1102 36-37 0.27793194257797893 1102 38-39 0.29764483565380573 1102 40-41 0.31733860953048776 1102 42-43 0.3774882363502101 1102 44-45 0.35138249382251985 1102 46-47 0.1462744416160362 1102 48-49 0.17112568014809426 1102 50-51 0.09724499690129562 1102 52-53 0.12359501602450251 1102 54-55 0.25856709584464 1102 56-57 0.2603935879584185 1102 58-59 0.26600755691082156 1102 60-61 0.266490485692529 1102 62-63 0.10902401979113563 1102 64-65 0.03417104507487778 1102 66-67 0.08500530260230477 1102 68-69 0.12101598886356868 1102 70-71 0.16998677844797072 1102 72-73 -0.008314944700948956 1102 74-75 -0.007483524918406204 1102 76-77 -0.002950296325327173 1102 78-79 0.04149594331129336 1102 80-81 -0.17697472976058748 1102 82-83 -0.1548822321446508 1102 84-85 -0.10409062730866836 1102 86-87 0.07236925423238816 1102 88-89 0.29136309989437237 1102 90-91 0.3139746804897783 1102 92-93 0.28502828384834444 1102 94-95 0.13012903933593734 1102 96-97 0.00537623194532344 1102 98-99 -0.02463190242258051 1102 100-101 -0.3235746197279532 1103 1 0.039698144662118295 1103 2 0.029230894653018424 1103 3 0.1050733649663087 1103 4 0.09982155935957593 1103 5 0.18893242906965213 1103 6 0.15772825240291155 1103 7 0.09355874696865385 1103 8 0.2150477629865506 1103 9 0.17809095732817326 1103 10-11 0.17508196089600858 1103 12-13 0.22421966398639626 1103 14-15 0.2644483949003984 1103 16-17 0.2196518910898817 1103 18-19 0.2397771808768212 1103 20-21 0.2722003829832076 1103 22-23 0.25501652890466886 1103 24-25 0.10667778166276776 1103 26-27 0.18303219437977702 1103 28-29 0.26509854458659987 1103 30-31 0.2570289073966663 1103 32-33 0.3243109908023385 1103 34-35 0.2798696934106104 1103 36-37 0.3467415753334109 1103 38-39 0.345527290277829 1103 40-41 0.25123374061662673 1103 42-43 0.257008462764027 1103 44-45 0.3605347977864497 1103 46-47 0.39347053699454904 1103 48-49 0.41712191788261066 1103 50-51 0.29983875696365914 1103 52-53 0.47616674480244114 1103 54-55 0.5241745661447723 1103 56-57 0.5272941251828129 1103 58-59 0.5636205519791986 1103 60-61 0.5514482025735816 1103 62-63 0.4960526774080307 1103 64-65 0.40741150807078697 1103 66-67 0.30846692579955004 1103 68-69 0.4327586634614917 1103 70-71 0.43673140196721505 1103 72-73 0.3285239049270601 1103 74-75 0.24123611851373283 1103 76-77 0.29996715414664976 1103 78-79 0.4607936537524253 1103 80-81 0.3077152222790005 1103 82-83 0.5447135785218791 1103 84-85 0.41311072168382523 1103 86-87 0.4192806376275797 1103 88-89 0.35239738396236575 1103 90-91 0.4598429673032065 1103 92-93 0.5405971146650295 1103 94-95 0.37572108773697366 1103 96-97 0.4009140494923926 1103 98-99 0.18644898628554074 1103 100-101 -0.11509765936731853 1104 1 -0.0015586609061628565 1104 2 0.07775622760876644 1104 3 0.06305028225557407 1104 4 0.007915500495052186 1104 5 0.03559537540526492 1104 6 0.07244993554731849 1104 7 0.09462487168858758 1104 8 0.0037812678993347504 1104 9 0.14414487991752623 1104 10-11 0.10571194620903412 1104 12-13 0.08674815644717881 1104 14-15 0.07913620637400953 1104 16-17 0.07764269021144088 1104 18-19 0.08070554229309579 1104 20-21 0.2232455461363898 1104 22-23 0.1680549010654815 1104 24-25 0.15184926393705211 1104 26-27 0.03903983494026875 1104 28-29 0.13318310160344282 1104 30-31 0.2589234998677128 1104 32-33 0.3074826007894842 1104 34-35 0.380075422165163 1104 36-37 0.3660053940033663 1104 38-39 0.43035472522061724 1104 40-41 0.5982256863775319 1104 42-43 0.47551710975735517 1104 44-45 0.4516643069526225 1104 46-47 0.28639108016287906 1104 48-49 0.28115075877737183 1104 50-51 0.3565021752815696 1104 52-53 0.3023459370463826 1104 54-55 0.4116004024557611 1104 56-57 0.5028926403820364 1104 58-59 0.5999756825655993 1104 60-61 0.5003366842620522 1104 62-63 0.6750988844749912 1104 64-65 0.6020689460953506 1104 66-67 0.490193437045221 1104 68-69 0.503114565877766 1104 70-71 0.37399745838852994 1104 72-73 0.4779412844258175 1104 74-75 0.44626300762288196 1104 76-77 0.49388524578339954 1104 78-79 0.45134984324498717 1104 80-81 0.3296890522487672 1104 82-83 0.47419192702805546 1104 84-85 0.32607718595337687 1104 86-87 0.4114199112745851 1104 88-89 0.6895640464644828 1104 90-91 0.690746424712362 1104 92-93 0.5040992788426841 1104 94-95 0.41345857118288976 1104 96-97 0.43400556130722023 1104 98-99 0.3578521341298284 1104 100-101 0.026461214871609684 1105 1 -0.03964156561782772 1105 2 0.0326763733657458 1105 3 0.05717665972206021 1105 4 0.03350881055290955 1105 5 0.07474332127998196 1105 6 0.05364427588678211 1105 7 0.05006058214787146 1105 8 0.042480412846138904 1105 9 -0.05221479878976254 1105 10-11 0.030022755952941793 1105 12-13 0.07279823669617969 1105 14-15 0.06848632461477422 1105 16-17 0.0926475835722087 1105 18-19 0.054409622990363005 1105 20-21 0.12104318937515046 1105 22-23 0.08158044695206712 1105 24-25 -0.011675884546107795 1105 26-27 -0.023427743564127468 1105 28-29 -0.013470417191875583 1105 30-31 -0.07246299827163938 1105 32-33 0.009244316639680505 1105 34-35 0.14693605702506574 1105 36-37 0.09366085689622139 1105 38-39 0.09594398706231999 1105 40-41 0.056497532570752185 1105 42-43 -0.031046140176009374 1105 44-45 0.004502800613508384 1105 46-47 -0.011656185410167552 1105 48-49 -0.005026861475570854 1105 50-51 -0.014717922098164138 1105 52-53 -0.1474416110857959 1105 54-55 -0.08407637821623126 1105 56-57 -0.1129451575579381 1105 58-59 0.12911409605696633 1105 60-61 0.12401207243246404 1105 62-63 0.08423471219478529 1105 64-65 -0.099841398633572 1105 66-67 -0.048903641191856195 1105 68-69 -0.03164207221647786 1105 70-71 0.014327758915598565 1105 72-73 -0.09876605338987332 1105 74-75 -0.09409516413064267 1105 76-77 0.03575355052929652 1105 78-79 0.12303500859925265 1105 80-81 -0.09501465700877176 1105 82-83 -0.027560357114328582 1105 84-85 0.06458997519020926 1105 86-87 0.2011317726009274 1105 88-89 0.4741997414515282 1105 90-91 0.3066356201611917 1105 92-93 0.20004380344486705 1105 94-95 0.061522342244611394 1105 96-97 0.0654697915553406 1105 98-99 0.02313142130466872 1105 100-101 -0.3415539564013166 1106 1 -0.0433399256477216 1106 2 -5.667304746594937E-4 1106 3 0.02328018740417548 1106 4 -0.06422232044975118 1106 5 -0.11398828083841295 1106 6 -0.03580628539075548 1106 7 -0.015664631478742308 1106 8 -0.013132565580008304 1106 9 -0.009658021221369495 1106 10-11 -0.076787506247328 1106 12-13 -0.05663878000407152 1106 14-15 -0.08308043979486968 1106 16-17 -0.1666681208270404 1106 18-19 -0.09917359797915992 1106 20-21 -0.12149003240664769 1106 22-23 -0.06566225684610316 1106 24-25 -0.13777473153096764 1106 26-27 -0.13474051161273337 1106 28-29 -0.11472780883526212 1106 30-31 -1.4571807150076665E-4 1106 32-33 0.08381234209618071 1106 34-35 -0.11035151445455682 1106 36-37 -0.03629702698187742 1106 38-39 0.04071556700578327 1106 40-41 0.0075611474092696085 1106 42-43 0.054064876621701785 1106 44-45 0.25225865503875156 1106 46-47 0.2414888167017324 1106 48-49 0.239639580463475 1106 50-51 0.13938032786935395 1106 52-53 0.12075624939896556 1106 54-55 0.11029135198066342 1106 56-57 0.1449896903854082 1106 58-59 -0.06368037158225093 1106 60-61 0.044964386179302096 1106 62-63 0.06179604862015253 1106 64-65 0.0354442051256072 1106 66-67 -0.0815553120281649 1106 68-69 -0.1392057134537268 1106 70-71 -0.022600505591519493 1106 72-73 -0.07036127765720579 1106 74-75 -0.07291602440421485 1106 76-77 0.04928086711906943 1106 78-79 0.136549633493658 1106 80-81 0.02346790358645734 1106 82-83 0.011015289451400179 1106 84-85 0.13273321061811316 1106 86-87 0.19400665482199386 1106 88-89 0.23402590583842908 1106 90-91 0.4339795224615344 1106 92-93 0.35270662266639263 1106 94-95 0.24687947639887398 1106 96-97 0.38933495092242154 1106 98-99 0.49888647564542765 1106 100-101 -0.0052765727993921985 1107 1 -0.003938122724491677 1107 2 0.012110951825370364 1107 3 0.03474872659466399 1107 4 0.023168259376781464 1107 5 0.0875626748313536 1107 6 0.12344072312193788 1107 7 0.03768071465039213 1107 8 0.08198970393492999 1107 9 0.05424135237323924 1107 10-11 0.13503087634181554 1107 12-13 0.043355196867118195 1107 14-15 0.09090516492726408 1107 16-17 0.06454674425487639 1107 18-19 0.09505172726979794 1107 20-21 0.19263420466371173 1107 22-23 0.10984243115582615 1107 24-25 0.09168062704597446 1107 26-27 0.049783560825432005 1107 28-29 0.1021851368762654 1107 30-31 0.145139175921436 1107 32-33 0.10083194738047041 1107 34-35 0.09870877706487846 1107 36-37 0.07213033408461911 1107 38-39 0.13601576978445706 1107 40-41 0.12364014074367446 1107 42-43 0.30675370171004346 1107 44-45 0.25259419350646084 1107 46-47 0.3480886601003803 1107 48-49 0.21757585576061444 1107 50-51 0.2092338152609301 1107 52-53 0.3302938738769683 1107 54-55 0.21539093437706214 1107 56-57 0.2073340928910241 1107 58-59 0.28979146025199043 1107 60-61 0.3225761654115473 1107 62-63 0.3206698741100382 1107 64-65 0.1737129069405725 1107 66-67 0.16777662260080461 1107 68-69 0.10246128783121122 1107 70-71 0.20789509752444246 1107 72-73 0.22509753418025014 1107 74-75 0.07301309340818563 1107 76-77 0.07334627829800056 1107 78-79 0.3320619005993457 1107 80-81 0.27457114001434135 1107 82-83 0.2968237941092866 1107 84-85 0.4290289863971033 1107 86-87 0.35162139542591575 1107 88-89 0.2895011808946464 1107 90-91 0.4326900709677979 1107 92-93 0.2830581726182082 1107 94-95 0.258085005631127 1107 96-97 0.16806376958609093 1107 98-99 0.3202111122499929 1107 100-101 -0.045232252609860524 1108 1 0.013268672863304687 1108 2 0.08360918992490696 1108 3 0.03699243295923793 1108 4 0.08057471259667182 1108 5 0.12202608273512539 1108 6 0.07595658999405686 1108 7 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100-101 -0.445102388192506 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 7.0 5 24.0 6 49.0 7 144.0 8 277.0 9 554.0 10 1025.0 11 1602.0 12 2502.0 13 3579.0 14 4500.0 15 6257.0 16 8696.0 17 13228.0 18 13153.0 19 7941.0 20 6673.0 21 7049.0 22 7177.0 23 7857.0 24 8759.0 25 10381.0 26 12530.0 27 14530.0 28 18242.0 29 22572.0 30 27345.0 31 33682.0 32 42691.0 33 55414.0 34 76951.0 35 114146.0 36 186497.0 37 334976.0 38 345612.0 39 40066.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.2580994387928 20.039322919395 16.12819411413425 21.574383527677956 2 15.480621120865766 21.339910474958813 39.723239411620995 23.45622899255443 3 19.01206451204259 25.4500439551108 30.79600165101382 24.741889881832794 4 13.02247592213507 18.062174730439974 37.5674552601008 31.347894087324153 5 13.652882000538739 37.82499114285376 33.77931123768265 14.742815618924851 6 30.237218389732533 36.79763178354736 17.93967117540073 15.025478651319386 7 27.465505852676948 33.89234860892341 20.797205805276423 17.844939733123223 8 26.20044783401882 34.82100908127206 20.004830537908937 18.973712546800186 9 26.470373280860976 17.186970354835722 20.835696979104096 35.5069593851992 10-11 24.770845267569342 27.461294792972474 26.7497301093553 21.018129830102882 12-13 26.044046988465873 24.837178158451387 27.501004041921274 21.617770811161467 14-15 23.066348479253723 25.8521472604015 26.633323577039974 24.448180683304802 16-17 21.6039051458287 29.197549747906354 27.20397920605902 21.994565900205927 18-19 21.500112236960938 27.388846256244815 30.051907419298146 21.059134087496105 20-21 22.789489473394802 26.271792233970654 29.93704987774006 21.001668414894485 22-23 22.32662092736583 26.33513214026936 29.823942902206664 21.51430403015815 24-25 22.007855573273787 26.58783715590104 29.999993039541668 21.404314231283504 26-27 21.85256543219845 26.68670363157228 29.98535510392725 21.475375832302017 28-29 21.6013790002457 26.92273157870192 30.076989308084606 21.398900112967766 30-31 21.814747064703212 26.713462968067425 29.92144235347182 21.55034761375754 32-33 21.616413527455062 26.785715528648087 29.775707127409962 21.82216381648689 34-35 22.068322449550692 26.758895151617672 29.71662486486637 21.456157533965268 36-37 22.116619090719208 26.52896330850101 29.80571607602868 21.548701524751095 38-39 21.88637395258689 26.743182242222897 29.806299934001483 21.564143871188726 40-41 21.98895166081856 26.64135292207374 29.8221969912402 21.547498425867495 42-43 21.862896332040417 26.75890398371652 29.411617293679015 21.966582390564046 44-45 21.967730596420157 26.957585502434473 29.155016340039886 21.919667561105484 46-47 22.08001158226313 27.119966561783613 28.761668834280712 22.038353021672545 48-49 22.175954428407906 27.197028992795563 28.609586864757013 22.017429714039512 50-51 22.12517440243782 27.38016477441399 28.507372668384267 21.987288154763913 52-53 22.34458661126163 27.470039691861693 28.147533310015888 22.037840386860793 54-55 22.02753128190922 27.278687931242096 28.368203923872393 22.32557686297629 56-57 22.133712630325338 27.606837368874217 28.091457256351916 22.167992744448537 58-59 22.248587845903796 27.289360206315454 28.271725246844188 22.190326700936563 60-61 22.164367522444415 27.269901688003685 28.52088581733028 22.04484497222162 62-63 22.15890285093787 27.246112001503516 28.484842156919044 22.11014299063957 64-65 21.925462920265126 27.315192972625884 28.433858217019598 22.325485890089393 66-67 21.90705727784497 27.46580121986235 28.19507356587866 22.432067936414022 68-69 21.863408339000465 27.43631525350347 28.329784560855938 22.370491846640128 70-71 21.99137185188485 27.398720533892767 28.181873232485994 22.42803438173639 72-73 22.01228794182195 27.28554395406674 28.13687405729686 22.565294046814454 74-75 21.88637377644354 27.634783492367543 27.890892487121466 22.587950244067454 76-77 21.866749349016008 27.351930351513914 28.1383427304288 22.64297756904127 78-79 21.863421696474873 27.503349725235527 28.069409787114086 22.563818791175517 80-81 21.822828988101847 27.73598886020722 27.90168666513765 22.539495486553278 82-83 21.90987519537157 27.58951071755092 27.901374378734943 22.59923970834257 84-85 21.818822810440366 27.466584719212804 27.75258875765386 22.96200371269297 86-87 21.843509125951652 27.471609002061125 27.88264961278985 22.802232259197375 88-89 21.83163633834878 27.27158361309842 27.880328218876883 23.016451829675916 90-91 21.64037907796549 27.38608740680022 27.985074757241186 22.988458757993108 92-93 21.794774527334653 27.39437026560302 27.89684365414184 22.914011552920492 94-95 21.557667504470334 27.389254350094276 27.8347914272569 23.218286718178483 96-97 21.540022816287127 27.32591444379558 27.86019699406902 23.273865745848276 98-99 21.422040970310256 27.25784441778566 27.700846642715522 23.619267969188567 100-101 21.596023062838825 26.771623673550927 28.27101445795125 23.361338805659003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 701.0 1 470.5 2 286.5 3 373.5 4 335.0 5 308.0 6 625.5 7 1341.0 8 2210.5 9 2719.5 10 2432.5 11 1923.5 12 1829.5 13 1932.5 14 2089.0 15 2204.0 16 2283.0 17 2388.5 18 2485.0 19 2633.5 20 2872.5 21 3179.5 22 3788.5 23 4615.0 24 5495.0 25 6763.5 26 8398.5 27 10301.5 28 12293.5 29 14373.0 30 16766.5 31 19726.0 32 22802.5 33 25750.5 34 28725.5 35 31392.5 36 34740.0 37 38313.5 38 40473.5 39 41348.5 40 42643.5 41 45233.5 42 47487.0 43 49363.0 44 51909.5 45 53745.5 46 54014.5 47 55696.5 48 62191.0 49 62428.0 50 56148.5 51 52126.0 52 48371.5 53 46717.0 54 44964.5 55 41240.0 56 37199.0 57 33005.5 58 29190.0 59 25498.0 60 21419.5 61 17336.5 62 13745.5 63 10649.0 64 7986.0 65 5971.5 66 4446.0 67 3445.5 68 2558.0 69 1734.5 70 1177.5 71 878.5 72 723.5 73 615.0 74 494.5 75 386.0 76 269.0 77 143.0 78 83.5 79 56.5 80 28.5 81 9.5 82 6.5 83 6.5 84 4.0 85 2.0 86 2.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3450980828889679 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.396579505851285E-4 16-17 0.0017053051695804183 18-19 3.480214631796772E-5 20-21 0.0 22-23 0.0 24-25 4.1762575581561265E-4 26-27 0.0010440643895390316 28-29 0.0 30-31 0.002853775998073353 32-33 0.0 34-35 0.010579852480662188 36-37 0.02735448700592263 38-39 0.020672474912872826 40-41 0.013050804869237894 42-43 0.01009262243221064 44-45 0.002644963120165547 46-47 9.396579505851285E-4 48-49 5.916364874054513E-4 50-51 1.0440643895390316E-4 52-53 3.480214631796772E-5 54-55 0.0 56-57 0.0 58-59 0.0040370489728842555 60-61 0.010023018139574704 62-63 0.004211059704474095 64-65 0.0017749094622163538 66-67 0.0038630382412944172 68-69 0.0032017974612530303 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.21535568141558425 78-79 5.568343410874835E-4 80-81 0.0028885781443913207 82-83 5.220321947695158E-4 84-85 0.0 86-87 0.10823467504887962 88-89 0.4092384385529824 90-91 0.3963616444153344 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0012876794137648057 100-101 6.612407800413867E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1436693.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.073952505776404 #Duplication Level Percentage of deduplicated Percentage of total 1 77.59496038138823 46.61435964639119 2 13.450464757916341 16.160451620953715 3 4.038561690674916 7.27837089591759 4 1.7502501468455829 4.205777767793189 5 0.8909616260262789 2.6761793203186004 6 0.49543703873259043 1.7857716680654487 7 0.33195818693593243 1.3959428249124999 8 0.23579556098637322 1.1332137065414631 9 0.1780349195972416 0.9625735173828973 >10 0.9448865959602013 10.283883350280773 >50 0.05964284966940286 2.472676012043176 >100 0.02754189195517203 3.143266787663769 >500 6.943169131419797E-4 0.3153998484249807 >1k 5.785974276183164E-4 0.6348316984388194 >5k 2.314389710473266E-4 0.937301334871945 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7070 0.49210234893606364 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6413 0.44637232867425397 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3728 0.25948480294676735 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1615 0.11241093260703575 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1598 0.11122765963222485 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.3920858527187088E-4 0.0 22-23 0.0 0.0 0.0 1.7401073158983862E-4 0.0 24-25 0.0 0.0 0.0 2.7841717054374177E-4 0.0 26-27 0.0 0.0 0.0 7.656472189952899E-4 0.0 28-29 0.0 0.0 0.0 0.0029929845833452243 0.0 30-31 0.0 0.0 0.0 0.007691274336270867 0.0 32-33 0.0 0.0 0.0 0.01754028174425573 0.0 34-35 0.0 0.0 0.0 0.03160034885671469 0.0 36-37 0.0 0.0 0.0 0.05418694181707574 0.0 38-39 0.0 0.0 0.0 0.08992874608562859 0.0 40-41 0.0 0.0 0.0 0.13290939678831873 0.0 42-43 0.0 0.0 0.0 0.17794337412376895 0.0 44-45 0.0 0.0 0.0 0.23268715028193218 0.0 46-47 0.0 0.0 0.0 0.3016998064304622 0.0 48-49 0.0 0.0 0.0 0.37610679525827717 0.0 50-51 0.0 0.0 0.0 0.45862268417817864 0.0 52-53 0.0 0.0 0.0 0.5467069165089549 0.0 54-55 0.0 0.0 0.0 0.6441877283455826 0.0 56-57 0.0 0.0 0.0 0.7609141270960462 0.0 58-59 0.0 0.0 0.0 0.9004359316847789 0.0 60-61 0.0 0.0 0.0 1.0486234707066855 0.0 62-63 0.0 0.0 0.0 1.2019617273836514 0.0 64-65 0.0 0.0 0.0 1.3657754301023253 0.0 66-67 0.0 0.0 0.0 1.5575352563143274 0.0 68-69 0.0 0.0 0.0 1.779920971286141 0.0 70-71 0.0 0.0 0.0 2.02277034829292 0.0 72-73 0.0 0.0 0.0 2.280549846070107 0.0 74-75 0.0 0.0 0.0 2.5526678281302964 0.0 76-77 0.0 0.0 0.0 2.841108016813613 0.0 78-79 0.0 0.0 0.0 3.1793848790242594 0.0 80-81 0.0 0.0 0.0 3.5525682939918273 0.0 82-83 0.0 0.0 0.0 3.944022835776328 0.0 84-85 0.0 0.0 0.0 4.343725486238187 0.0 86-87 0.0 0.0 0.0 4.771896292388144 0.0 88-89 0.0 0.0 0.0 5.232502698906448 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7150 0.0 31.765137 1 GTATCAA 10435 0.0 29.796078 1 ATCAACG 12385 0.0 25.057417 3 TCAACGC 12565 0.0 24.698458 4 TATCAAC 12615 0.0 24.675797 2 CAACGCA 12720 0.0 24.4348 5 AACGCAG 12965 0.0 23.973055 6 GAGTACT 6415 0.0 23.414074 12-13 GTACTTT 6735 0.0 22.3016 14-15 ACGCAGA 14185 0.0 21.877768 7 CGCAGAG 14295 0.0 21.70942 8 TACCTGG 1155 0.0 20.541992 2 GCAGAGT 15125 0.0 20.394016 9 AGTACTT 6655 0.0 20.109486 12-13 GTACATG 8335 0.0 19.422792 1 ACTTTTT 7910 0.0 18.777163 16-17 TACATGG 8315 0.0 18.775358 2 ACCGTCC 155 9.691692E-4 18.368519 8 AGAGTAC 12740 0.0 18.271303 10-11 GACCGGG 130 0.0076082465 18.250772 6 >>END_MODULE