FastQCFastQC Report
Fri 27 May 2016
SRR939128_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939128_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413379
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71440.5054553661827437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63320.44800439231090877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33600.23772816774552333No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15390.10888799111915487No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15210.10761444736337529No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC600.004010156731.5710853
GGTATCA70950.030.5142271
GTATCAA102050.029.4496861
TTGTGCG950.001252372324.9113039
ATCAACG122500.024.3548373
TATCAAC123400.024.292342
TCAACGC124150.023.9930084
CAACGCA124750.023.9147245
AACGCAG126750.023.5257036
GAGTACT63450.023.27534112-13
GTACATG79400.022.0645871
GTACTTT66200.021.95212414-15
TACATGG78250.021.4239562
ACGCAGA141200.021.1516727
CATAGCG1353.8748232E-421.0473922
CGCAGAG141800.020.8952778
AGTACTT65400.020.77194812-13
CATGGGG30900.020.2300154
ACATGGG81250.020.2249223
GCAGAGT148850.019.5240369
Produced by FastQC (version 0.11.5)