##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939128_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1413379 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.906054922281992 33.0 31.0 34.0 30.0 34.0 2 32.02525225010418 34.0 31.0 34.0 30.0 34.0 3 32.06441655069164 34.0 31.0 34.0 30.0 34.0 4 35.51959028682328 37.0 35.0 37.0 33.0 37.0 5 35.56536569455185 37.0 35.0 37.0 33.0 37.0 6 35.65296640179315 37.0 35.0 37.0 33.0 37.0 7 35.5802604963 37.0 35.0 37.0 33.0 37.0 8 35.57671933713463 37.0 35.0 37.0 33.0 37.0 9 37.203610638052496 39.0 37.0 39.0 34.0 39.0 10-11 37.10551593026358 39.0 37.0 39.0 33.0 39.0 12-13 36.99766976868908 39.0 37.0 39.0 33.0 39.0 14-15 38.21596118238632 40.0 38.0 41.0 33.0 41.0 16-17 38.11596500301759 40.0 38.0 41.0 33.0 41.0 18-19 38.03483142172057 40.0 38.0 41.0 33.0 41.0 20-21 38.03873837095358 40.0 38.0 41.0 33.0 41.0 22-23 38.0794454282963 40.0 38.0 41.0 33.0 41.0 24-25 38.02057374561247 40.0 38.0 41.0 33.0 41.0 26-27 37.8921856770194 40.0 38.0 41.0 33.0 41.0 28-29 37.74444328096003 40.0 38.0 41.0 32.5 41.0 30-31 37.509676809971 40.0 37.5 41.0 32.0 41.0 32-33 37.27496340330513 40.0 37.0 41.0 31.5 41.0 34-35 36.94959632200563 40.0 36.0 41.0 30.5 41.0 36-37 36.67772267735689 40.0 36.0 41.0 30.0 41.0 38-39 36.48382740935021 40.0 36.0 41.0 30.0 41.0 40-41 36.28893736216542 39.5 35.0 41.0 30.0 41.0 42-43 36.052642992431615 39.0 35.0 41.0 29.0 41.0 44-45 35.74394376879803 39.0 35.0 41.0 27.5 41.0 46-47 35.525239868428784 39.0 35.0 41.0 26.0 41.0 48-49 35.35246490856309 39.0 35.0 41.0 25.0 41.0 50-51 34.23695555120035 38.0 34.0 40.0 23.5 40.5 52-53 34.384487812540016 38.5 34.5 39.5 23.5 40.5 54-55 34.91044298804496 39.0 35.0 40.5 24.0 41.0 56-57 34.82903771741338 39.0 35.0 41.0 23.0 41.0 58-59 34.629571756761635 39.0 35.0 41.0 23.0 41.0 60-61 34.33624632883324 38.0 34.0 40.0 22.5 41.0 62-63 33.95696023501128 38.0 34.0 40.0 21.5 41.0 64-65 33.589094644819255 37.0 34.0 40.0 20.0 41.0 66-67 33.20492132683448 37.0 33.0 40.0 18.5 41.0 68-69 32.796340542770196 36.0 33.0 39.0 16.0 41.0 70-71 32.364018426763096 36.0 32.5 39.0 12.5 40.5 72-73 31.90516556422587 35.0 32.0 38.5 8.0 40.0 74-75 31.451626209247486 35.0 31.5 37.0 7.0 39.5 76-77 30.99791669467284 35.0 31.0 37.0 3.5 39.0 78-79 30.55473867943418 35.0 31.0 36.0 2.0 39.0 80-81 30.095471915176326 35.0 30.5 36.0 2.0 37.5 82-83 29.681663587756717 34.0 30.0 35.5 2.0 37.0 84-85 29.285002465722215 34.0 29.5 35.0 2.0 37.0 86-87 28.850519570476145 34.0 29.0 35.0 2.0 36.0 88-89 28.407914649927584 34.0 29.0 35.0 2.0 36.0 90-91 27.973564061727252 34.0 28.0 35.0 2.0 35.5 92-93 27.535387889589416 34.0 27.0 35.0 2.0 35.0 94-95 27.02562157779336 34.0 26.0 35.0 2.0 35.0 96-97 26.387874731406086 33.0 25.0 35.0 2.0 35.0 98-99 25.62208827214781 33.0 22.0 35.0 2.0 35.0 100-101 22.68591651637671 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2837629074353032 1101 2 0.42560833062618997 1101 3 0.43575799843691954 1101 4 0.5241914193964234 1101 5 0.277707819720888 1101 6 0.3386882950401571 1101 7 0.325526606757073 1101 8 0.3955160988494413 1101 9 0.31769949158779553 1101 10-11 0.4727868053287736 1101 12-13 0.49900336142703594 1101 14-15 0.6890920699807026 1101 16-17 0.7185937564960838 1101 18-19 0.8121647022555933 1101 20-21 0.782119982724133 1101 22-23 0.7518266527802169 1101 24-25 0.6774859555342374 1101 26-27 0.7286011336531928 1101 28-29 0.6132428247414623 1101 30-31 0.7617033594184406 1101 32-33 0.830652335747935 1101 34-35 0.9355354880396618 1101 36-37 0.7456448391494206 1101 38-39 0.633400369803077 1101 40-41 0.7467659823853694 1101 42-43 0.7632936287853411 1101 44-45 0.6365451987135202 1101 46-47 1.035785060167676 1101 48-49 1.1034896039619184 1101 50-51 1.0479272169915532 1101 52-53 1.0235187999509847 1101 54-55 1.001473567895637 1101 56-57 1.0585350677561038 1101 58-59 0.8658311685946032 1101 60-61 0.8857322659744398 1101 62-63 0.8358902110784001 1101 64-65 0.7023719067660821 1101 66-67 0.840040974954718 1101 68-69 0.8780077612585302 1101 70-71 0.7652549783987155 1101 72-73 0.8468065122157817 1101 74-75 0.8680150888103846 1101 76-77 0.5436825153717102 1101 78-79 0.6589588493998484 1101 80-81 0.524778677354842 1101 82-83 0.37192606890397784 1101 84-85 0.1833436253470211 1101 86-87 0.21236524379883548 1101 88-89 0.369408475823775 1101 90-91 0.04608531685391881 1101 92-93 0.15574842366909536 1101 94-95 0.18769092444841817 1101 96-97 0.2597610638538228 1101 98-99 0.38864063196198373 1101 100-101 0.3049762871178814 1102 1 0.38378726475907854 1102 2 0.4584785085323304 1102 3 0.4967434872102672 1102 4 0.2672228745298071 1102 5 0.43814871780410414 1102 6 0.39055657502850494 1102 7 0.4443183125694432 1102 8 0.4708675249737979 1102 9 0.5358702993047331 1102 10-11 0.5669660154067273 1102 12-13 0.5721900990779005 1102 14-15 0.7017676456895501 1102 16-17 0.7258231200740042 1102 18-19 0.7445142194738992 1102 20-21 0.8023289307136139 1102 22-23 0.8589640811962411 1102 24-25 0.8584071507401774 1102 26-27 0.7929142357567756 1102 28-29 0.7885435217027776 1102 30-31 0.7663614686674478 1102 32-33 0.9352639890486003 1102 34-35 0.9301515427514673 1102 36-37 1.0783845008695394 1102 38-39 1.02560181402756 1102 40-41 0.9493209374525122 1102 42-43 1.1766984591051468 1102 44-45 0.99104651150612 1102 46-47 1.2340951868929082 1102 48-49 1.2866475218319522 1102 50-51 1.204029581239162 1102 52-53 1.2715526633460783 1102 54-55 1.2586038844614862 1102 56-57 1.3478460485138228 1102 58-59 1.1022586756438812 1102 60-61 1.059177777298153 1102 62-63 1.158511510856009 1102 64-65 1.1760281486202615 1102 66-67 1.2204828375635444 1102 68-69 1.08665265772769 1102 70-71 1.012413138010615 1102 72-73 1.081996618380746 1102 74-75 0.9741190097290477 1102 76-77 0.8234318730159735 1102 78-79 0.9439767755355035 1102 80-81 1.1411749411000933 1102 82-83 0.9254062665218434 1102 84-85 1.0354253829324378 1102 86-87 0.9959934594692044 1102 88-89 1.0640390594655464 1102 90-91 1.1838085846910822 1102 92-93 1.1244416318827 1102 94-95 0.9430145868864344 1102 96-97 0.9879049442135788 1102 98-99 1.0915038689465355 1102 100-101 0.6954840121743651 1103 1 0.22227833763719929 1103 2 0.36301824190783805 1103 3 0.4072913476424773 1103 4 0.38463026795879074 1103 5 0.37940817795331583 1103 6 0.4330837386042461 1103 7 0.44632162066373127 1103 8 0.45985951473679876 1103 9 0.3911768192218972 1103 10-11 0.47184093146129413 1103 12-13 0.5314353000347509 1103 14-15 0.577216877345414 1103 16-17 0.6084432841128304 1103 18-19 0.617758554167736 1103 20-21 0.6460091945943418 1103 22-23 0.6875635208211222 1103 24-25 0.6818443577548692 1103 26-27 0.6894068889957694 1103 28-29 0.5991599163592696 1103 30-31 0.5399609951582605 1103 32-33 0.7479783457105285 1103 34-35 0.6082446738129832 1103 36-37 0.5170128219188257 1103 38-39 0.6154481452000269 1103 40-41 0.47134504544423805 1103 42-43 0.4679982669816667 1103 44-45 0.24075281479761657 1103 46-47 0.6998235816883849 1103 48-49 0.775351655871475 1103 50-51 0.7234186561409572 1103 52-53 0.6159772783952135 1103 54-55 0.7185299345957148 1103 56-57 0.7482985900611965 1103 58-59 0.6105753397045746 1103 60-61 0.6381892310204833 1103 62-63 0.5658722902291302 1103 64-65 0.5519530461334412 1103 66-67 0.5280613209323164 1103 68-69 0.5543343378226382 1103 70-71 0.4838330485207294 1103 72-73 0.3927409156323627 1103 74-75 0.21048510230177442 1103 76-77 0.44781519978942796 1103 78-79 0.4578079285339669 1103 80-81 0.4186765451244412 1103 82-83 0.5256061180709644 1103 84-85 0.41625191526243555 1103 86-87 0.3630526403294638 1103 88-89 0.26582652069787827 1103 90-91 0.2337549973977069 1103 92-93 -0.05665885762179457 1103 94-95 -0.27609364147391346 1103 96-97 -0.24164067507760834 1103 98-99 -0.059672205899548914 1103 100-101 -0.4882335381584504 1104 1 0.19245049789200408 1104 2 0.35142744519307456 1104 3 0.4321489510124792 1104 4 0.4065532485594545 1104 5 0.29813132808142484 1104 6 0.2572054387590015 1104 7 0.2625256186859062 1104 8 0.3357577212135183 1104 9 0.4799347096326798 1104 10-11 0.40652149424102646 1104 12-13 0.38130719122894874 1104 14-15 0.6089872190331178 1104 16-17 0.5441031247313433 1104 18-19 0.48873468547444077 1104 20-21 0.5059531991664414 1104 22-23 0.5703198829163298 1104 24-25 0.6074706139596486 1104 26-27 0.6755043544320785 1104 28-29 0.6397116511258787 1104 30-31 0.741104663244073 1104 32-33 0.703952582676493 1104 34-35 0.7635099778386163 1104 36-37 0.6311645104506809 1104 38-39 0.40790866906551315 1104 40-41 0.4901375477078176 1104 42-43 0.6403575881013168 1104 44-45 0.8807799135024084 1104 46-47 0.9789980344165556 1104 48-49 0.8932053357086289 1104 50-51 0.7954325008820717 1104 52-53 0.7668695449899516 1104 54-55 0.8087583471381734 1104 56-57 0.727560375108105 1104 58-59 0.8457657691098746 1104 60-61 0.8342053361274893 1104 62-63 0.8620958095931499 1104 64-65 0.98611667864418 1104 66-67 0.8827653863801856 1104 68-69 0.7921511582840779 1104 70-71 0.8889426313162616 1104 72-73 0.958814878400549 1104 74-75 1.0177022194362415 1104 76-77 0.867626793154745 1104 78-79 0.7606391652495006 1104 80-81 0.7715344310401804 1104 82-83 0.6681151242163317 1104 84-85 0.7312775691310662 1104 86-87 1.0059276716342467 1104 88-89 0.8501535802315487 1104 90-91 1.042144037191708 1104 92-93 1.0064547964009485 1104 94-95 0.81151480150643 1104 96-97 0.885835613555706 1104 98-99 1.0860314935231585 1104 100-101 0.9638621164165428 1105 1 0.07225033628844102 1105 2 0.23553557782139478 1105 3 0.2518753590189391 1105 4 0.2206649251255186 1105 5 0.21918827207302627 1105 6 0.24938609452924254 1105 7 0.31630388158938416 1105 8 0.3317493858719516 1105 9 0.4092419406832448 1105 10-11 0.2546586588073225 1105 12-13 0.32959688687557787 1105 14-15 0.36805530516497953 1105 16-17 0.34958914035302513 1105 18-19 0.24079853305676835 1105 20-21 0.26596198813204097 1105 22-23 0.34897091845493833 1105 24-25 0.37581219285478085 1105 26-27 0.30921542427599746 1105 28-29 0.30277956714873255 1105 30-31 0.4729703338279805 1105 32-33 0.35005836213016295 1105 34-35 0.42050897136029874 1105 36-37 0.44824033654257533 1105 38-39 0.46351106760257466 1105 40-41 0.24934654080422547 1105 42-43 0.22754526086828264 1105 44-45 0.06761886279574014 1105 46-47 0.3661715314325633 1105 48-49 0.32136886717170654 1105 50-51 0.23658016967981865 1105 52-53 0.23768961218301143 1105 54-55 0.33347972275304727 1105 56-57 0.4235662155175035 1105 58-59 0.3781304261275764 1105 60-61 0.3496489662538025 1105 62-63 0.4974056206303814 1105 64-65 0.541852329240271 1105 66-67 0.5181508959275476 1105 68-69 0.3935675993200505 1105 70-71 0.24010461762249236 1105 72-73 0.49354518473852593 1105 74-75 0.43965195222333975 1105 76-77 0.2781279059511945 1105 78-79 0.2810771959541789 1105 80-81 0.280869446562626 1105 82-83 0.25930801365965195 1105 84-85 0.03569218149515763 1105 86-87 0.16057122237634758 1105 88-89 0.15040789320378067 1105 90-91 0.22570526934797996 1105 92-93 0.27169727262112886 1105 94-95 0.010922822146326894 1105 96-97 -0.13580026810512535 1105 98-99 -0.3725126568532069 1105 100-101 -0.7511993354638733 1106 1 0.2188912948340942 1106 2 0.2639228523014978 1106 3 0.3457301999499407 1106 4 0.22842418693519306 1106 5 0.2958664155794324 1106 6 0.29103325056835416 1106 7 0.3426969403967135 1106 8 0.22555464685528648 1106 9 0.2148554925356052 1106 10-11 0.3668635892576688 1106 12-13 0.32025244396137253 1106 14-15 0.45371430530269663 1106 16-17 0.4504912199563833 1106 18-19 0.4951946217497891 1106 20-21 0.5327227648845536 1106 22-23 0.49668335475848124 1106 24-25 0.41598021378854355 1106 26-27 0.47854334054097336 1106 28-29 0.44506024474134165 1106 30-31 0.5340294165989476 1106 32-33 0.5706781114759778 1106 34-35 0.3975217795134469 1106 36-37 0.39480279350441805 1106 38-39 0.4656636636637188 1106 40-41 0.6702926914721772 1106 42-43 0.6513766890462165 1106 44-45 0.4158890735105203 1106 46-47 0.4499602657666699 1106 48-49 0.7546590475876229 1106 50-51 0.8546410428377911 1106 52-53 0.6379003269605974 1106 54-55 0.4423986044497852 1106 56-57 0.5347864331183843 1106 58-59 0.6643857628580605 1106 60-61 0.7221555080524453 1106 62-63 0.6844638770688931 1106 64-65 0.6696763204260563 1106 66-67 0.4931612250833126 1106 68-69 0.5157284587058015 1106 70-71 0.3914005823656339 1106 72-73 0.5851909635568724 1106 74-75 0.4917590999991326 1106 76-77 0.5663926077828982 1106 78-79 0.5106227131005774 1106 80-81 0.5537258024364746 1106 82-83 0.5347948612013091 1106 84-85 0.6339995771706839 1106 86-87 0.7332141090310813 1106 88-89 0.6410730908349613 1106 90-91 0.5765081890560104 1106 92-93 0.4470381347613248 1106 94-95 0.6031924819727941 1106 96-97 0.7866305265512601 1106 98-99 0.5478647016373586 1106 100-101 0.49139259402242885 1107 1 0.19995008426201366 1107 2 0.25179605054123755 1107 3 0.1402807809929243 1107 4 0.10598881314405162 1107 5 0.08830890418256132 1107 6 0.07904347481668594 1107 7 0.10129400487799245 1107 8 0.1904865291183242 1107 9 0.20052046394459921 1107 10-11 0.19638742387781605 1107 12-13 0.10957100308019818 1107 14-15 0.09318438356562808 1107 16-17 0.14668743066022927 1107 18-19 0.17258928300567788 1107 20-21 0.2138197634879404 1107 22-23 0.20287324118499583 1107 24-25 0.2822191133642846 1107 26-27 0.26611481805026926 1107 28-29 0.3428994499904974 1107 30-31 0.49778471976898686 1107 32-33 0.4961465032649599 1107 34-35 0.38754391448406267 1107 36-37 0.41193763236710623 1107 38-39 0.3345811581958529 1107 40-41 0.4402695024553367 1107 42-43 0.6669186005590362 1107 44-45 0.6327261443160737 1107 46-47 0.8450312821307406 1107 48-49 0.7268875648081092 1107 50-51 0.792049143182993 1107 52-53 0.7022274502049513 1107 54-55 0.7103450589018152 1107 56-57 0.6691707383701697 1107 58-59 0.6811819893725897 1107 60-61 0.6308368962313224 1107 62-63 0.5808181947057207 1107 64-65 0.489620192464848 1107 66-67 0.6152764323351505 1107 68-69 0.6499915486422978 1107 70-71 0.6921146986796316 1107 72-73 0.5482308328551362 1107 74-75 0.66553574760513 1107 76-77 0.6624096306293517 1107 78-79 0.6500084676659128 1107 80-81 0.5077654345743454 1107 82-83 0.42192363929823173 1107 84-85 0.4369495890287034 1107 86-87 0.4765960163277114 1107 88-89 0.6024765533767926 1107 90-91 0.501734150214407 1107 92-93 0.6425439451911146 1107 94-95 0.5036154180185406 1107 96-97 0.6479351722599738 1107 98-99 0.5435325716734596 1107 100-101 0.6227847255673922 1108 1 -0.0780565192812901 1108 2 -0.01308055056106383 1108 3 -0.11781241376021612 1108 4 -0.0107243181431258 1108 5 0.0037501467792679932 1108 6 0.035460089023303 1108 7 -0.1010519231510969 1108 8 0.13635289087430635 1108 9 0.09457599023672003 1108 10-11 0.1022689455191923 1108 12-13 0.15274198860122112 1108 14-15 0.1528038447473321 1108 16-17 0.05075747446972656 1108 18-19 0.06384023049852061 1108 20-21 -0.032701813654036016 1108 22-23 0.10166815876917923 1108 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-1.891473376623587 2316 64-65 -2.270823062734216 2316 66-67 -2.31944543678571 2316 68-69 -2.3360805094584123 2316 70-71 -2.2822753540313343 2316 72-73 -2.5857944379029867 2316 74-75 -2.316326212361652 2316 76-77 -2.2785403108970677 2316 78-79 -2.074355526164698 2316 80-81 -2.1626007456598906 2316 82-83 -2.2191409189295968 2316 84-85 -2.228651205873078 2316 86-87 -2.3223400186547423 2316 88-89 -2.6640471072391314 2316 90-91 -2.8029801766614106 2316 92-93 -2.7507305332075553 2316 94-95 -2.528413700131999 2316 96-97 -2.583306247376985 2316 98-99 -2.6923348288790727 2316 100-101 -2.394046133231612 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5387.0 3 1584.0 4 1561.0 5 1713.0 6 2073.0 7 2414.0 8 3003.0 9 3472.0 10 4168.0 11 5175.0 12 6622.0 13 9702.0 14 8424.0 15 8874.0 16 11064.0 17 15794.0 18 13398.0 19 9048.0 20 8774.0 21 9335.0 22 9743.0 23 10616.0 24 12037.0 25 13231.0 26 15252.0 27 17892.0 28 20718.0 29 24141.0 30 28891.0 31 34850.0 32 43845.0 33 56555.0 34 77841.0 35 115677.0 36 181282.0 37 289789.0 38 282234.0 39 47175.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15773920937537 19.478850638561756 15.661686933901986 21.701723218160883 2 15.202799382322524 20.90009491832774 40.15767775936079 23.73942793998895 3 18.943544793907577 25.50213533693981 30.528057597783132 25.026262271369486 4 12.799110770431843 17.676058882412992 37.760802063534 31.76402828362116 5 13.637500380793282 37.66148291885313 33.78674300952089 14.914273690832694 6 30.267189840867058 36.446654073480396 18.14473762464564 15.141418461006909 7 27.389045571346536 33.94877321530105 20.819758841976988 17.842422371375424 8 26.165188231428928 34.451010547304264 20.194235801923664 19.189565419343143 9 26.68248942160089 17.099125405717714 20.864936359671233 35.35344881301016 10-11 24.77678982345686 27.27171670398047 26.8798863734524 21.071607099110274 12-13 26.0621339933505 24.77231071740755 27.557156426829827 21.608398862412123 14-15 23.131626487790218 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21.602556990330207 40-41 22.01025980120302 26.62613938540733 29.757867269598854 21.605733543790794 42-43 21.811102473593735 26.702849408717288 29.39265766768539 22.093390450003593 44-45 21.99209379328986 26.886971119050116 29.20022121729976 21.920713870360263 46-47 22.101837003748834 27.039729255955336 28.761575741151507 22.096857999144323 48-49 22.14562273215834 27.157512784562776 28.577454639610128 22.119409843668752 50-51 22.079791838717686 27.329846432684395 28.462911313079147 22.12745041551877 52-53 22.290591906959374 27.42272844478825 28.17157032729601 22.115109320956368 54-55 21.996119999075344 27.274983951875186 28.344313629258977 22.384582419790494 56-57 22.109481546160552 27.608658863147355 28.13444361172775 22.147415978964343 58-59 22.157642304514813 27.23991593472143 28.327382501283303 22.27505925948045 60-61 22.175129700833594 27.146894321444893 28.647309662561106 22.030666315160413 62-63 22.13794908337849 27.22157506140916 28.597965212617215 22.04251064259513 64-65 21.952656997270477 27.15752759576622 28.56440126595155 22.325414141011752 66-67 21.88667002946121 27.37569695576977 28.29197546630316 22.44565754846586 68-69 21.923775626160296 27.33235326975254 28.417349117764886 22.326521986322277 70-71 22.02466653361097 27.214621326693354 28.26832281873154 22.492389320964136 72-73 21.963379605339213 27.175889943175253 28.290971178557772 22.569759272927765 74-75 21.817057085087644 27.528340492862053 28.11674276778511 22.537859654265198 76-77 21.94380588755772 27.242117062650966 28.182123378674035 22.631953671117284 78-79 21.93762874612562 27.3109951768866 28.156835446008223 22.594540630979562 80-81 21.846539817854836 27.555127463926883 28.02531713980747 22.573015578410814 82-83 21.932282009648393 27.39298467963503 28.058329901702443 22.61640340901413 84-85 21.86461012846015 27.266152105408015 27.995892233718816 22.873345532413023 86-87 21.851208624119288 27.277624754374326 28.04961656436264 22.821550057143753 88-89 21.903186273638152 27.0465532950623 28.09439541032066 22.95586502097889 90-91 21.789983612755943 27.10692756781214 28.250655471977172 22.85243334745475 92-93 21.906490085920012 27.043975265225072 28.19427107437899 22.855263574475927 94-95 21.66391052088843 27.01461569462877 28.17265934216035 23.148814442322447 96-97 21.685487422356825 26.816535515416938 28.280399735634365 23.21757732659187 98-99 21.59657062119963 26.805592411599445 28.04803435354815 23.549802613652776 100-101 21.91605203450949 26.067219539257035 28.719514363544548 23.297214062688923 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1616.0 1 931.0 2 313.5 3 413.5 4 377.0 5 384.0 6 739.0 7 1452.0 8 2401.5 9 2961.0 10 2692.0 11 2334.0 12 2344.5 13 2467.5 14 2618.0 15 2757.0 16 2825.0 17 2913.5 18 3023.0 19 3025.0 20 3102.0 21 3450.5 22 3964.5 23 4657.5 24 5483.0 25 6577.5 26 8248.5 27 10148.0 28 11949.0 29 14031.5 30 16372.0 31 18983.0 32 21997.0 33 24988.5 34 28131.5 35 30903.0 36 33878.0 37 37354.0 38 39322.5 39 39967.5 40 41327.0 41 44030.5 42 46414.0 43 48278.0 44 50516.5 45 52186.0 46 52509.5 47 54308.5 48 60463.0 49 60498.5 50 54511.5 51 50607.5 52 47274.5 53 45663.5 54 43837.5 55 40348.5 56 36477.5 57 32384.0 58 28722.5 59 25034.5 60 21020.5 61 17325.5 62 13936.0 63 10758.5 64 8063.0 65 6230.5 66 4717.5 67 3735.5 68 2889.5 69 2010.0 70 1462.5 71 1105.5 72 857.5 73 686.5 74 543.5 75 434.5 76 314.0 77 194.0 78 115.5 79 77.5 80 56.5 81 39.0 82 30.5 83 24.0 84 17.0 85 11.0 86 6.5 87 5.0 88 5.5 89 4.0 90 4.5 91 5.5 92 4.0 93 1.5 94 1.0 95 1.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39663812749446536 2 0.11596323420681927 3 0.1173075303934755 4 0.12876942419549178 5 0.1310335019835444 6 0.06898362010472775 7 0.07853519827307467 8 0.07605886319239213 9 0.07549284374537898 10-11 0.07308726109557309 12-13 0.0772970307327334 14-15 0.07128307410821867 16-17 0.07298113244925812 18-19 0.08260346304848169 20-21 0.08175443387796195 22-23 0.09144751690806217 24-25 0.10450134040480297 26-27 0.10634090360759571 28-29 0.09947791781256124 30-31 0.08964332992070775 32-33 0.08819290508773656 34-35 0.34566100104784353 36-37 0.543307916701748 38-39 0.5201011193742089 40-41 0.48953606923549875 42-43 0.5213039106991119 44-45 0.5321644088386767 46-47 0.5289097970183511 48-49 0.5364449309067136 50-51 0.5338978433951544 52-53 0.5335794574562095 54-55 0.5272117386773116 56-57 0.49562077829089013 58-59 0.4848664087976403 60-61 0.4317313332092807 62-63 0.5120353422542715 64-65 0.5404070670358057 66-67 0.5520104656995752 68-69 0.5680005150776968 70-71 0.5626587065465102 72-73 0.5477299436315383 74-75 0.5513736938216854 76-77 0.5257613138443404 78-79 0.49710657933929964 80-81 0.5050308515974837 82-83 0.5194643474963191 84-85 0.5138041530261876 86-87 0.5147946870584605 88-89 0.5265395905839835 90-91 0.5456781231361156 92-93 0.5538146526869298 94-95 0.5470931717536485 96-97 0.5483313392939898 98-99 0.5490388636027562 100-101 0.5227897117475214 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1413379.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.02844499616715 #Duplication Level Percentage of deduplicated Percentage of total 1 79.18677980711709 49.11832815689363 2 12.569684544381667 15.593559727607015 3 3.832696432575085 7.132085995649691 4 1.5478496986559491 3.840428395816578 5 0.7766762163703247 2.4088008983478955 6 0.45021273082594854 1.6755597366366937 7 0.31347388405641186 1.3611008302449616 8 0.222296338501284 1.1030956964460998 9 0.15506453372669873 0.8656570711010555 >10 0.8640310274332789 9.709303751113994 >50 0.05377494048809328 2.2880947056277696 >100 0.025972474112015122 3.0266490536025956 >500 8.008924841118171E-4 0.3845086131849591 >1k 4.576528480638955E-4 0.5364526775683105 >5k 2.2882642403194775E-4 0.9563746901588241 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7144 0.5054553661827437 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6332 0.44800439231090877 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3360 0.23772816774552333 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1539 0.10888799111915487 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1521 0.10761444736337529 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 7.075243087664384E-5 0.0 20-21 0.0 0.0 0.0 1.0612864631496576E-4 0.0 22-23 0.0 0.0 0.0 2.1225729262993153E-4 0.0 24-25 0.0 0.0 0.0 4.5989080069818496E-4 0.0 26-27 0.0 0.0 0.0 0.0010612864631496578 0.0 28-29 0.0 0.0 0.0 0.0026178399424358224 0.0 30-31 0.0 0.0 0.0 0.007252124164855994 0.0 32-33 0.0 0.0 0.0 0.016096178024436473 0.0 34-35 0.0 0.0 0.0 0.028973120443985652 0.0 36-37 0.0 0.0 0.0 0.05256905614134638 0.0 38-39 0.0 0.0 0.0 0.08769763807160005 0.0 40-41 0.0 0.0 0.0 0.12933544364250493 0.0 42-43 0.0 0.0 0.0 0.17387409887935223 0.0 44-45 0.0 0.0 0.0 0.22679691717508185 0.0 46-47 0.0 0.0 0.0 0.2927028065366756 0.0 48-49 0.0 0.0 0.0 0.3645519000919074 0.0 50-51 0.0 0.0 0.0 0.44506816642952807 0.0 52-53 0.0 0.0 0.0 0.5315983893916636 0.0 54-55 0.0 0.0 0.0 0.6331988801305242 0.0 56-57 0.0 0.0 0.0 0.7555652093316796 0.0 58-59 0.0 0.0 0.0 0.8908792333832609 0.0 60-61 0.0 0.0 0.0 1.0422540592438405 0.0 62-63 0.0 0.0 0.0 1.203180463272767 0.0 64-65 0.0 0.0 0.0 1.3653450348420346 0.0 66-67 0.0 0.0 0.0 1.5550323020223167 0.0 68-69 0.0 0.0 0.0 1.7727021556143114 0.0 70-71 0.0 0.0 0.0 2.014250954627174 0.0 72-73 0.0 0.0 0.0 2.2702686257543094 0.0 74-75 0.0 0.0 0.0 2.5327247680912195 0.0 76-77 0.0 0.0 0.0 2.8200857660967085 0.0 78-79 0.0 0.0 0.0 3.152197676631675 0.0 80-81 0.0 0.0 0.0 3.526371907322806 0.0 82-83 0.0 0.0 0.0 3.915970167945045 0.0 84-85 0.0 0.0 0.0 4.312431414362319 0.0 86-87 0.0 0.0 0.0 4.730542904627846 0.0 88-89 0.0 0.0 0.0 5.190115319387086 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCGC 60 0.0040101567 31.571085 3 GGTATCA 7095 0.0 30.514227 1 GTATCAA 10205 0.0 29.449686 1 TTGTGCG 95 0.0012523723 24.911303 9 ATCAACG 12250 0.0 24.354837 3 TATCAAC 12340 0.0 24.29234 2 TCAACGC 12415 0.0 23.993008 4 CAACGCA 12475 0.0 23.914724 5 AACGCAG 12675 0.0 23.525703 6 GAGTACT 6345 0.0 23.275341 12-13 GTACATG 7940 0.0 22.064587 1 GTACTTT 6620 0.0 21.952124 14-15 TACATGG 7825 0.0 21.423956 2 ACGCAGA 14120 0.0 21.151672 7 CATAGCG 135 3.8748232E-4 21.047392 2 CGCAGAG 14180 0.0 20.895277 8 AGTACTT 6540 0.0 20.771948 12-13 CATGGGG 3090 0.0 20.230015 4 ACATGGG 8125 0.0 20.224922 3 GCAGAGT 14885 0.0 19.524036 9 >>END_MODULE