FastQCFastQC Report
Fri 27 May 2016
SRR939128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939128_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413379
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68300.4832391028874774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61230.43321713425769026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35330.2499683382871827No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16030.11341614669526008No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15510.1097370202896746No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77200.029.454841
GTATCAA106600.026.4293161
GAGTACT61450.023.09052812-13
GTACTTT63750.021.92248214-15
ATCAACG128350.021.8512443
TCAACGC129600.021.6404884
TATCAAC130000.021.5739022
CAACGCA131350.021.3882965
AGTACTT62850.021.17932712-13
AACGCAG134200.020.9694376
GTATAGG4350.020.8218821
TACCTGG11450.020.7228322
GTACATG82650.020.3027761
GTAAGGT4450.020.2618064
TACATGG83650.019.9124972
ATCGTAT1951.0413529E-519.4688348
ACGCAGA146250.019.209257
CGCAGAG146550.019.1699288
ACATGGG84650.019.0605983
ACTTTTT76250.018.20415716-17
Produced by FastQC (version 0.11.5)