##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939128_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1413379 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50669353372309 34.0 31.0 34.0 31.0 34.0 2 32.72999174319132 34.0 31.0 34.0 31.0 34.0 3 32.820566175102364 34.0 31.0 34.0 31.0 34.0 4 36.2717530117541 37.0 37.0 37.0 35.0 37.0 5 36.243686229949645 37.0 37.0 37.0 35.0 37.0 6 36.28214583632557 37.0 37.0 37.0 35.0 37.0 7 36.23664919317466 37.0 37.0 37.0 35.0 37.0 8 36.21396030364113 37.0 37.0 37.0 35.0 37.0 9 37.95664432540741 39.0 38.0 39.0 35.0 39.0 10-11 37.884156337401365 39.0 38.0 39.0 35.0 39.0 12-13 37.7996821800805 39.0 38.0 39.0 35.0 39.0 14-15 39.201701737467445 40.5 39.0 41.0 36.0 41.0 16-17 39.29320054988789 41.0 39.0 41.0 36.0 41.0 18-19 39.34977242480609 41.0 39.0 41.0 36.0 41.0 20-21 39.32741501041122 41.0 39.0 41.0 36.0 41.0 22-23 39.26033993712939 41.0 39.0 41.0 36.0 41.0 24-25 39.19061023264107 41.0 39.0 41.0 36.0 41.0 26-27 39.05045285093382 40.5 39.0 41.0 35.0 41.0 28-29 38.9061928895222 40.0 38.0 41.0 35.0 41.0 30-31 38.708051060614316 40.0 38.0 41.0 35.0 41.0 32-33 38.586966765460645 40.0 38.0 41.0 35.0 41.0 34-35 38.481853416528764 40.0 38.0 41.0 34.0 41.0 36-37 38.334915121846294 40.0 38.0 41.0 34.0 41.0 38-39 38.20893475847596 40.0 38.0 41.0 34.0 41.0 40-41 38.01698801241564 40.0 38.0 41.0 33.0 41.0 42-43 37.82486332399165 40.0 37.5 41.0 33.0 41.0 44-45 37.539948591283725 40.0 37.0 41.0 33.0 41.0 46-47 37.18954859241576 40.0 37.0 41.0 32.0 41.0 48-49 36.81046768064334 40.0 36.5 41.0 31.0 41.0 50-51 36.54114607617631 40.0 36.0 41.0 30.5 41.0 52-53 36.27148981271124 39.0 36.0 41.0 30.0 41.0 54-55 36.03781823559003 39.0 35.0 41.0 30.0 41.0 56-57 35.809800838982326 39.0 35.0 40.0 29.0 41.0 58-59 35.484893294721374 38.5 35.0 40.0 28.0 41.0 60-61 35.122068461467165 38.0 34.5 40.0 28.0 41.0 62-63 34.73665449960697 38.0 34.0 40.0 26.5 41.0 64-65 34.29111972089581 37.0 34.0 40.0 26.0 41.0 66-67 33.869474146708 36.5 33.5 39.0 26.0 41.0 68-69 33.396492023724704 36.0 33.0 39.0 24.5 40.0 70-71 32.892765846952585 35.5 32.5 38.5 23.5 40.0 72-73 32.318345610059296 35.0 32.0 37.5 22.0 39.5 74-75 31.773178673236266 35.0 31.0 37.0 20.0 39.0 76-77 29.782723883685833 33.0 28.5 35.0 18.0 37.5 78-79 30.74802229267592 34.0 30.5 36.0 18.0 38.0 80-81 30.7580769206278 34.0 31.0 36.0 18.0 37.0 82-83 30.37395878953911 34.0 31.0 35.0 13.5 37.0 84-85 29.900058300003042 34.0 30.0 35.0 7.5 36.5 86-87 29.204515915405565 34.0 29.0 35.0 2.0 36.0 88-89 28.527463971093386 34.0 29.0 35.0 2.0 35.5 90-91 27.979746409137253 33.0 27.5 35.0 2.0 35.0 92-93 27.524200161457046 33.0 27.0 35.0 2.0 35.0 94-95 26.996451765591537 33.0 26.0 34.5 2.0 35.0 96-97 26.213761843072525 32.0 25.0 34.0 2.0 35.0 98-99 25.027292042686355 32.0 15.0 34.0 2.0 35.0 100-101 19.20789399021777 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14691073339980676 1101 2 0.19109447500797927 1101 3 0.21107684612938016 1101 4 0.18055601582339875 1101 5 0.18153221941594921 1101 6 0.1998926244169752 1101 7 0.15414753751151267 1101 8 0.17747408504803985 1101 9 0.2164794834216579 1101 10-11 0.19057322219340023 1101 12-13 0.1470726399880462 1101 14-15 0.14154122737934216 1101 16-17 0.16362977163289116 1101 18-19 0.1937417174648104 1101 20-21 0.19688837277304572 1101 22-23 0.23580058821252692 1101 24-25 0.15319811956413076 1101 26-27 0.16572157457724046 1101 28-29 0.2157612173500354 1101 30-31 0.21333794229589387 1101 32-33 0.19168284545222747 1101 34-35 0.12008708095859788 1101 36-37 0.29957814500068025 1101 38-39 0.24152369824035702 1101 40-41 0.26141577145109096 1101 42-43 0.24687707678609172 1101 44-45 0.3407626128236032 1101 46-47 0.2744711368902557 1101 48-49 0.4236736469839286 1101 50-51 0.38056220034572164 1101 52-53 0.36124692396447955 1101 54-55 0.3069989075822406 1101 56-57 0.29198690227438817 1101 58-59 0.12145203754617029 1101 60-61 0.15965252981001044 1101 62-63 -0.026062189733217167 1101 64-65 0.20199418859294838 1101 66-67 0.31209708362516864 1101 68-69 0.4243462284462609 1101 70-71 0.1761610262036939 1101 72-73 0.26742779242811565 1101 74-75 0.1304197970227925 1101 76-77 0.18077691300604215 1101 78-79 0.4925539330875104 1101 80-81 0.5834398840783663 1101 82-83 0.5563956648202755 1101 84-85 0.6009230166390509 1101 86-87 0.8472050499575055 1101 88-89 0.909023079242214 1101 90-91 1.0488813803377148 1101 92-93 0.8075179538592536 1101 94-95 0.3014972502378548 1101 96-97 0.11541026560588108 1101 98-99 0.2338521038206558 1101 100-101 -0.45610821674236846 1102 1 0.20663928659308795 1102 2 0.21615504782630524 1102 3 0.210664786140768 1102 4 0.11148377346619753 1102 5 0.140171774366749 1102 6 0.18168934026834904 1102 7 0.22212217415090407 1102 8 0.18687271554377105 1102 9 0.22472984008247465 1102 10-11 0.26034840836820194 1102 12-13 0.24983185471769076 1102 14-15 0.34914712221796407 1102 16-17 0.2770067039653199 1102 18-19 0.21247152271000402 1102 20-21 0.31646177164527955 1102 22-23 0.21530808190496487 1102 24-25 0.2585829194952751 1102 26-27 0.1648608270454801 1102 28-29 0.23665601629833333 1102 30-31 0.16467518378929213 1102 32-33 0.24924854154974696 1102 34-35 0.3020280483289426 1102 36-37 0.30164851752122956 1102 38-39 0.30706840186544326 1102 40-41 0.4167129399579892 1102 42-43 0.39634954843178605 1102 44-45 0.4849857454440425 1102 46-47 0.5728831492630349 1102 48-49 0.6509607477970007 1102 50-51 0.402061353943985 1102 52-53 0.48760405116770045 1102 54-55 0.22998794800745515 1102 56-57 0.28357141638851147 1102 58-59 0.21176581970134833 1102 60-61 0.23334564340243702 1102 62-63 0.1284252045886518 1102 64-65 0.3342550671292557 1102 66-67 0.3051586038762295 1102 68-69 0.36249297185985796 1102 70-71 0.3178492268201367 1102 72-73 0.2654886686446858 1102 74-75 0.23830015433679463 1102 76-77 0.4545031745353292 1102 78-79 0.6540338328012645 1102 80-81 0.540645470268803 1102 82-83 0.4432513174592003 1102 84-85 0.3286868861204226 1102 86-87 0.5643573064285263 1102 88-89 0.5669525480022664 1102 90-91 0.7437765980610571 1102 92-93 0.5236656996469335 1102 94-95 0.2672727685024867 1102 96-97 0.22722259090007668 1102 98-99 0.049287993085791015 1102 100-101 -0.30278313681798963 1103 1 0.08171649243359269 1103 2 0.06659348114831687 1103 3 0.11388715274016192 1103 4 0.12150211116379239 1103 5 0.14863611051034553 1103 6 0.15504066982630604 1103 7 0.11081636353900848 1103 8 0.09763863941687134 1103 9 0.06694836001879878 1103 10-11 0.15046540180053825 1103 12-13 0.15593247901414742 1103 14-15 0.0664545299648509 1103 16-17 0.21160264858438893 1103 18-19 0.15134995773338744 1103 20-21 0.20077522226559097 1103 22-23 0.2906501988816217 1103 24-25 0.25638880589021085 1103 26-27 0.26266857057411386 1103 28-29 0.14120144089002196 1103 30-31 0.23146549961584384 1103 32-33 0.3076137606705558 1103 34-35 0.44550091231067057 1103 36-37 0.3554409942126995 1103 38-39 0.3381523269354929 1103 40-41 0.40557433128660847 1103 42-43 0.17961575536800467 1103 44-45 0.15063458302810773 1103 46-47 0.45786546755299184 1103 48-49 0.24718004293688267 1103 50-51 0.41461827359419345 1103 52-53 0.2247974668499424 1103 54-55 0.3242940958988001 1103 56-57 0.26470144030959375 1103 58-59 0.230832891582601 1103 60-61 0.2828053073570871 1103 62-63 0.23832109127714318 1103 64-65 0.23981622403968572 1103 66-67 0.18083009250069182 1103 68-69 0.3184600322987805 1103 70-71 0.22139778996777437 1103 72-73 -0.038810176517692696 1103 74-75 -0.06766741531620468 1103 76-77 0.01583681219895894 1103 78-79 -0.07313585350489049 1103 80-81 -0.10018607033143567 1103 82-83 -0.08355490980963154 1103 84-85 -0.2532103137413131 1103 86-87 -0.2915006623824148 1103 88-89 -0.11926823883532833 1103 90-91 0.0993338310276961 1103 92-93 0.16048762868165767 1103 94-95 0.28153885023194647 1103 96-97 0.11233640623748542 1103 98-99 -0.21290304127178317 1103 100-101 -0.8176488930098849 1104 1 0.012377199953803597 1104 2 0.06863551624820019 1104 3 0.1884856488262585 1104 4 0.005369391725288608 1104 5 0.04457579983846216 1104 6 0.07726353419793242 1104 7 0.06198090148040336 1104 8 0.12516758430118813 1104 9 0.10032728595971463 1104 10-11 0.09815912222153855 1104 12-13 0.18468150776318026 1104 14-15 0.19377991348857648 1104 16-17 0.18023365027324445 1104 18-19 0.26480491416354823 1104 20-21 0.17447920423403218 1104 22-23 0.241317071036093 1104 24-25 0.21320425857343395 1104 26-27 0.14826644377529163 1104 28-29 0.17534131387287033 1104 30-31 0.21128595464291067 1104 32-33 0.19080036286542423 1104 34-35 0.18128532991327262 1104 36-37 0.19241852540510962 1104 38-39 0.18707062626701543 1104 40-41 0.2966079991797841 1104 42-43 0.25231027847574694 1104 44-45 0.31680679465486605 1104 46-47 0.3883560054815476 1104 48-49 0.40870951438370895 1104 50-51 0.2943629549917688 1104 52-53 0.2885725347572432 1104 54-55 0.23504817194213956 1104 56-57 0.28948519649830473 1104 58-59 0.20092157241509057 1104 60-61 0.17742859041011627 1104 62-63 0.12813788605261323 1104 64-65 0.21971373699505392 1104 66-67 0.26025755209344226 1104 68-69 0.33385235347622455 1104 70-71 0.35537905204820674 1104 72-73 0.1858875316510975 1104 74-75 0.0626824918932094 1104 76-77 -0.06712246910742792 1104 78-79 0.23964548820206844 1104 80-81 0.44744536159751647 1104 82-83 0.22281457011356665 1104 84-85 -0.062247924431815704 1104 86-87 0.12879254303475918 1104 88-89 0.3821027501389693 1104 90-91 0.6822834800930444 1104 92-93 0.36438290695710407 1104 94-95 0.35801242257328525 1104 96-97 0.1278378928133499 1104 98-99 -0.05522325549282314 1104 100-101 -0.4581929219836667 1105 1 -0.0622121994196263 1105 2 -0.033384528263653124 1105 3 0.0036820538558188787 1105 4 -0.009197042931589294 1105 5 -0.044659985427266236 1105 6 -0.011645641765667847 1105 7 0.0671799999026419 1105 8 0.01431718100106849 1105 9 0.07573462616355897 1105 10-11 -0.03129355203388684 1105 12-13 0.022039998329219657 1105 14-15 0.02772148104312322 1105 16-17 0.1311124050752781 1105 18-19 0.04013768991734423 1105 20-21 0.06739614850916098 1105 22-23 0.0029285704052739447 1105 24-25 0.08039930273090334 1105 26-27 0.07643819717393541 1105 28-29 0.14222590908429567 1105 30-31 0.15932757464773317 1105 32-33 0.18160710485257425 1105 34-35 0.23876856708776018 1105 36-37 0.30251903147942016 1105 38-39 0.3924163019541851 1105 40-41 0.4585537627566083 1105 42-43 0.3007434327220935 1105 44-45 0.33673506535501474 1105 46-47 0.36320595515762477 1105 48-49 0.28225444442637837 1105 50-51 0.3514574930749248 1105 52-53 0.37835862267525044 1105 54-55 0.287315016278221 1105 56-57 0.3994958283115295 1105 58-59 0.38255064971545494 1105 60-61 0.27430198677829054 1105 62-63 0.3450132670259194 1105 64-65 0.3289115312482025 1105 66-67 0.23189839526710898 1105 68-69 0.36406059525783263 1105 70-71 0.29473405537950725 1105 72-73 0.08478777689158079 1105 74-75 0.05443957622475182 1105 76-77 -0.07970203050969715 1105 78-79 0.04982906417700761 1105 80-81 0.3054760662740996 1105 82-83 0.2822693707316297 1105 84-85 0.24130933851418845 1105 86-87 0.45535336409425753 1105 88-89 0.4637564932460094 1105 90-91 0.36544750770741175 1105 92-93 0.1578909800472772 1105 94-95 0.18085069143058163 1105 96-97 0.11062172678695603 1105 98-99 0.046670341320467656 1105 100-101 -0.8395603149213073 1106 1 0.07325398981050313 1106 2 0.007794372714172937 1106 3 0.07324547487220201 1106 4 0.007525171639407802 1106 5 -0.0010131048323032132 1106 6 0.04130023345813072 1106 7 0.07856686121650824 1106 8 0.10487495541230629 1106 9 0.07646179872931924 1106 10-11 0.11725485727613005 1106 12-13 0.1697180041135482 1106 14-15 0.16416549701888528 1106 16-17 0.13100498185533382 1106 18-19 0.016408176076275538 1106 20-21 0.18451775710300922 1106 22-23 0.1797265321185364 1106 24-25 0.22965147185360735 1106 26-27 0.29707722761050803 1106 28-29 0.2110759297426128 1106 30-31 0.25014839822574686 1106 32-33 0.2821703881468878 1106 34-35 0.14072008891670862 1106 36-37 0.27089253375183375 1106 38-39 0.18904899717522738 1106 40-41 0.22105479567218822 1106 42-43 0.20458725588694904 1106 44-45 0.2948124651621953 1106 46-47 0.3168996612587165 1106 48-49 0.12848636840379157 1106 50-51 0.16919747379383665 1106 52-53 0.2206654674624744 1106 54-55 0.15118087497536692 1106 56-57 0.2814804034389198 1106 58-59 0.16513293865637735 1106 60-61 0.1978138997379446 1106 62-63 0.14285378486092526 1106 64-65 0.18050497567734425 1106 66-67 0.22047159455095056 1106 68-69 -0.19346040735889147 1106 70-71 -0.05680705466043179 1106 72-73 0.10721719432719823 1106 74-75 0.024355565757876008 1106 76-77 0.09152094153272117 1106 78-79 0.22544399325013842 1106 80-81 0.5295333586580675 1106 82-83 0.4767894092938114 1106 84-85 0.2973236616101822 1106 86-87 0.2942997902063631 1106 88-89 0.25911525925619827 1106 90-91 0.565312540072096 1106 92-93 0.4716177394468062 1106 94-95 -0.0625675705168085 1106 96-97 -0.03838529703896043 1106 98-99 0.10422302001486372 1106 100-101 -0.39539973097970105 1107 1 0.021387010443461918 1107 2 0.07194889589001008 1107 3 0.09085595209162989 1107 4 0.008766311063368448 1107 5 0.041786441927818885 1107 6 0.0478353200216759 1107 7 0.07642187715895687 1107 8 0.025449937625275254 1107 9 0.14167563835619035 1107 10-11 0.14308557745320627 1107 12-13 0.12114360162599525 1107 14-15 0.15605197624099532 1107 16-17 0.12266747625571384 1107 18-19 0.1873006897129983 1107 20-21 0.13871417861609814 1107 22-23 0.03935524433756399 1107 24-25 0.12923248848118618 1107 26-27 0.05805116631772478 1107 28-29 0.18393423957950006 1107 30-31 0.23951965965556354 1107 32-33 0.29847516561137866 1107 34-35 0.10475998456613667 1107 36-37 0.1505784279782958 1107 38-39 0.17497879769697988 1107 40-41 0.145558735169935 1107 42-43 0.2280753583402415 1107 44-45 0.29034857942080805 1107 46-47 0.17132665538240843 1107 48-49 0.14082266185025816 1107 50-51 0.03273669748879371 1107 52-53 0.15124466586031815 1107 54-55 0.047479855185649456 1107 56-57 0.0029315615722111943 1107 58-59 -0.20696777545993683 1107 60-61 -0.16099397398732407 1107 62-63 -0.16445339055871244 1107 64-65 -0.13312647706185743 1107 66-67 -0.2410544008639519 1107 68-69 -0.21866265247734162 1107 70-71 -0.1744465181509085 1107 72-73 -0.24110555462534222 1107 74-75 -0.45623680821772794 1107 76-77 -0.2873309386222225 1107 78-79 -0.17727808111282073 1107 80-81 -0.32332442415571094 1107 82-83 -0.33422405614664896 1107 84-85 -0.2401687624326101 1107 86-87 -0.3605488224053026 1107 88-89 -0.3411614278765285 1107 90-91 0.2109975383592726 1107 92-93 0.3296653823359499 1107 94-95 0.24620999937963006 1107 96-97 0.09809260751714888 1107 98-99 -0.13795332191773113 1107 100-101 -0.8319790874844841 1108 1 -0.15850173141452473 1108 2 -0.11178377623249958 1108 3 -0.14650931121501998 1108 4 -0.05865920636407651 1108 5 -0.09046942610409303 1108 6 -0.028840296972809654 1108 7 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-0.9587320600802798 2316 94-95 -0.9187132646328457 2316 96-97 -0.8645662880947889 2316 98-99 -0.8841906738270175 2316 100-101 0.503984286017662 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 4.0 4 11.0 5 29.0 6 50.0 7 142.0 8 306.0 9 575.0 10 993.0 11 1701.0 12 2551.0 13 3728.0 14 4820.0 15 6530.0 16 8967.0 17 13494.0 18 13702.0 19 8832.0 20 7754.0 21 8246.0 22 8570.0 23 9303.0 24 10690.0 25 12428.0 26 14297.0 27 17289.0 28 21088.0 29 25685.0 30 30993.0 31 38264.0 32 48093.0 33 62920.0 34 87780.0 35 132509.0 36 214793.0 37 343685.0 38 240269.0 39 12283.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.309170896496546 20.0305983157425 16.098579729361948 21.561651058399008 2 15.462660758367006 21.41152514647522 39.632540174999065 23.49327392015871 3 18.911841763603395 25.490049024359358 30.84197515316133 24.75613405887593 4 12.963472642511315 18.113471333591345 37.46857707663691 31.454478947260426 5 13.614890273592575 37.83217381891198 33.73872117811288 14.814214729382567 6 30.175699511595973 36.756949126879626 17.93842981960253 15.128921541921878 7 27.394562958696856 33.96689776769005 20.817770746558427 17.820768527054668 8 26.170758161823542 34.84132706089449 19.968387813884316 19.019526963397645 9 26.4659373034409 17.206495922183645 20.779422929023283 35.548143845352165 10-11 24.797028963922628 27.44256848304666 26.7316126813827 21.028789871648016 12-13 26.048179575329762 24.79607380610579 27.547600466683036 21.608146151881414 14-15 23.03415285419599 25.826846752063247 26.651190614748543 24.487809778992215 16-17 21.6298415250052 29.166318792425916 27.188919061574445 22.014920620994435 18-19 21.520766899748757 27.434608834573034 30.016046651322824 21.028577614355385 20-21 22.795831832792196 26.238645119249686 29.951414305717012 21.01410874224111 22-23 22.334632112122794 26.29885543792571 29.83035689648707 21.53615555346443 24-25 21.970929241201404 26.628632518241744 29.967581236172318 21.432857004384527 26-27 21.86382958851848 26.704801136604843 29.959768829970596 21.47160044490608 28-29 21.551693336215315 26.93298362752794 30.048957161864287 21.46636587439246 30-31 21.866781662950984 26.655306184682242 29.91858517779025 21.55932697457653 32-33 21.56923994528005 26.806336731455872 29.805214951267477 21.819208371996602 34-35 22.06234129596955 26.770683223836322 29.63481222993558 21.53216325025854 36-37 22.06961466532908 26.526680077919735 29.789931652149605 21.613773604601583 38-39 21.861752097814875 26.773973826630176 29.77957684557287 21.584697229982076 40-41 22.057154461534978 26.61877732162139 29.78157562171946 21.542492595124173 42-43 21.87360583221623 26.7260969104269 29.403535883689656 21.996761373667216 44-45 21.991422333842653 26.907501230257942 29.205527546280162 21.89554888961924 46-47 22.04927094775907 27.112889445502837 28.762459691185395 22.075379915552702 48-49 22.218947879340188 27.185921630627476 28.580131326699664 22.014999163332675 50-51 22.1656630949395 27.362503126396266 28.47488110850072 21.996952670163513 52-53 22.402872831703316 27.382216659508877 28.18412471106476 22.030785797723045 54-55 21.951012431909632 27.27378855919042 28.313212521199198 22.461986487700752 56-57 22.117225540513576 27.599835288224384 28.11374593350116 22.169193237760883 58-59 22.281580462982486 27.234536472712506 28.237219444754974 22.246663619550038 60-61 22.129432337459587 27.232101636645172 28.62185087400595 22.01661515188929 62-63 22.173598960209034 27.208016918536735 28.532554669330768 22.085829451923463 64-65 21.87717790065412 27.2866497564289 28.464140036579888 22.372032306337093 66-67 21.8688797631716 27.464120553807142 28.25037641225349 22.41662327076777 68-69 21.923512748877556 27.349953531417682 28.311094334229214 22.41543938547555 70-71 22.008848311526226 27.337150668222275 28.16226668468498 22.49173433556652 72-73 21.98543348953112 27.261371507571567 28.156778896530938 22.596416106366373 74-75 21.890625232156413 27.633989184783413 27.857000846906598 22.618384736153573 76-77 21.856184264994955 27.32950817175903 28.136078299308092 22.67822926393792 78-79 21.817332010523977 27.439224599725154 28.067075492126126 22.676367897624747 80-81 21.807678919126968 27.718950937330195 27.86855726996921 22.60481287357363 82-83 21.853409453515297 27.54745896182128 27.910878823019164 22.68825276164426 84-85 21.76022850205076 27.45686047408374 27.834466197672388 22.948444826193114 86-87 21.80744822506162 27.383913647052154 27.8363600305975 22.97227809728872 88-89 21.76271752433022 27.189773048209272 27.932672922628214 23.114836504832294 90-91 21.60542906819517 27.332283160211084 28.010419308974715 23.051868462619023 92-93 21.800132873065188 27.256348084979333 27.89092663751195 23.052592404443537 94-95 21.547794328343635 27.30442436176001 27.79558773690567 23.352193572990686 96-97 21.493004174041136 27.1956167438517 27.862564769451353 23.44881431265581 98-99 21.378973904343994 27.156413327795768 27.691585472241304 23.77302729561893 100-101 21.770132402983073 26.31935131134742 28.39863037008238 23.51188591558713 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 602.0 1 427.0 2 282.0 3 353.0 4 324.0 5 324.0 6 612.5 7 1278.5 8 2098.5 9 2557.5 10 2307.0 11 1858.5 12 1803.5 13 1926.5 14 2083.5 15 2182.5 16 2180.0 17 2273.5 18 2455.5 19 2648.5 20 2907.5 21 3248.0 22 3725.5 23 4480.5 24 5357.0 25 6528.0 26 8311.5 27 10179.5 28 11929.0 29 14030.5 30 16419.5 31 19358.0 32 22515.0 33 25409.5 34 28404.0 35 30953.5 36 34115.0 37 37642.0 38 39574.5 39 40434.5 40 41711.5 41 44022.5 42 46447.5 43 48622.5 44 50886.0 45 52809.0 46 53267.5 47 55117.0 48 61155.5 49 61150.5 50 55503.0 51 51421.5 52 47606.0 53 46028.0 54 44449.0 55 40740.0 56 36649.5 57 32615.0 58 28815.0 59 25257.0 60 21156.5 61 16997.5 62 13738.0 63 10670.0 64 7888.5 65 5933.0 66 4404.5 67 3415.0 68 2558.0 69 1737.0 70 1172.5 71 846.5 72 723.0 73 633.0 74 470.0 75 380.0 76 266.5 77 133.0 78 76.5 79 50.0 80 26.5 81 11.0 82 8.5 83 5.0 84 3.0 85 1.0 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4324388575180472 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.844053859580481E-4 16-17 0.002334830218929247 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0033253642512022603 28-29 3.537621543832192E-5 30-31 0.0 32-33 3.537621543832192E-5 34-35 0.012346299187974351 36-37 0.027345814533822847 38-39 0.021331857909308118 40-41 0.008561044136073906 42-43 0.008879430075018803 44-45 0.004068264775407021 46-47 0.005058798807680035 48-49 0.0023702064343675685 50-51 7.429005242047603E-4 52-53 0.0 54-55 0.0 56-57 7.075243087664384E-5 58-59 0.0014858010484095207 60-61 0.0021579491417376374 62-63 0.0012735437557795891 64-65 0.0024055826498058904 66-67 0.0024763350806825345 68-69 0.004139017206283665 70-71 1.4150486175328769E-4 72-73 0.0 74-75 0.0 76-77 0.09993780861325943 78-79 0.015919296947244865 80-81 2.1225729262993153E-4 82-83 0.0 84-85 0.0 86-87 0.10605789388408912 88-89 0.4189605194360465 90-91 0.4239485658128499 92-93 0.0 94-95 0.0 96-97 3.537621543832192E-5 98-99 1.4150486175328769E-4 100-101 3.183859389448973E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1413379.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.32738171141398 #Duplication Level Percentage of deduplicated Percentage of total 1 77.80360657139722 46.93687872157357 2 13.405395398679728 16.17424810417169 3 3.9916262920562757 7.224130889105849 4 1.6549631498954793 3.9935837464827437 5 0.8759447630758902 2.642172704009665 6 0.5128845118674553 1.8564587832780126 7 0.3242824809446867 1.369417910719211 8 0.23599698895147694 1.1389664348176076 9 0.1677750023175896 0.9109283945801933 >10 0.9382584543819685 10.258891599261805 >50 0.05905065744865788 2.4442887558502133 >100 0.028693265462916017 3.208728838146286 >500 7.026754710399879E-4 0.30330453808564367 >1k 5.855628925333233E-4 0.6228587951928802 >5k 2.3422515701332933E-4 0.9151417847247479 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6830 0.4832391028874774 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6123 0.43321713425769026 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3533 0.2499683382871827 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1603 0.11341614669526008 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1551 0.1097370202896746 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 7.075243087664384E-5 0.0 24-25 0.0 0.0 0.0 3.1838593894489733E-4 0.0 26-27 0.0 0.0 0.0 9.197816013963699E-4 0.0 28-29 0.0 0.0 0.0 0.0024763350806825345 0.0 30-31 0.0 0.0 0.0 0.007145995518541028 0.0 32-33 0.0 0.0 0.0 0.016308435317066404 0.0 34-35 0.0 0.0 0.0 0.02957451610643713 0.0 36-37 0.0 0.0 0.0 0.05334733288098946 0.0 38-39 0.0 0.0 0.0 0.08918343912000956 0.0 40-41 0.0 0.0 0.0 0.13156414521511922 0.0 42-43 0.0 0.0 0.0 0.17656269125266472 0.0 44-45 0.0 0.0 0.0 0.22983927170277751 0.0 46-47 0.0 0.0 0.0 0.29648806158857605 0.0 48-49 0.0 0.0 0.0 0.3700705897002856 0.0 50-51 0.0 0.0 0.0 0.4520019046554392 0.0 52-53 0.0 0.0 0.0 0.5404070670358057 0.0 54-55 0.0 0.0 0.0 0.6433164777458842 0.0 56-57 0.0 0.0 0.0 0.7670271031336959 0.0 58-59 0.0 0.0 0.0 0.9035439185101802 0.0 60-61 0.0 0.0 0.0 1.0572181983742506 0.0 62-63 0.0 0.0 0.0 1.2193827699435182 0.0 64-65 0.0 0.0 0.0 1.383952924162592 0.0 66-67 0.0 0.0 0.0 1.5760457739926799 0.0 68-69 0.0 0.0 0.0 1.7948830426941393 0.0 70-71 0.0 0.0 0.0 2.039368067588382 0.0 72-73 0.0 0.0 0.0 2.2974021829955023 0.0 74-75 0.0 0.0 0.0 2.5641388474004496 0.0 76-77 0.0 0.0 0.0 2.8539408042711827 0.0 78-79 0.0 0.0 0.0 3.1872201299156133 0.0 80-81 0.0 0.0 0.0 3.564755101073385 0.0 82-83 0.0 0.0 0.0 3.9574310924387586 0.0 84-85 0.0 0.0 0.0 4.355590397197071 0.0 86-87 0.0 0.0 0.0 4.77557682688083 0.0 88-89 0.0 0.0 0.0 5.238403853460396 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7720 0.0 29.45484 1 GTATCAA 10660 0.0 26.429316 1 GAGTACT 6145 0.0 23.090528 12-13 GTACTTT 6375 0.0 21.922482 14-15 ATCAACG 12835 0.0 21.851244 3 TCAACGC 12960 0.0 21.640488 4 TATCAAC 13000 0.0 21.573902 2 CAACGCA 13135 0.0 21.388296 5 AGTACTT 6285 0.0 21.179327 12-13 AACGCAG 13420 0.0 20.969437 6 GTATAGG 435 0.0 20.821882 1 TACCTGG 1145 0.0 20.722832 2 GTACATG 8265 0.0 20.302776 1 GTAAGGT 445 0.0 20.261806 4 TACATGG 8365 0.0 19.912497 2 ATCGTAT 195 1.0413529E-5 19.468834 8 ACGCAGA 14625 0.0 19.20925 7 CGCAGAG 14655 0.0 19.169928 8 ACATGGG 8465 0.0 19.060598 3 ACTTTTT 7625 0.0 18.204157 16-17 >>END_MODULE