FastQCFastQC Report
Fri 27 May 2016
SRR939127_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939127_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2575482
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48110.18679998540079099No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39610.15379645441125195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44300.043.1899721
GTATCAA72000.036.3329541
ATCAACG86300.030.0304783
TCAACGC87900.029.5377464
CAACGCA88300.029.3502855
TATCAAC88900.029.205492
AACGCAG90550.028.6098586
ACGCAGA103600.024.7317247
CGCAGAG105200.024.0854578
GCAGAGT118100.021.2925539
GTACATG82900.021.0753561
GTGGTAT24050.020.7279241
GAGTACT57650.020.65999612-13
TACATGG82050.020.0949252
AGAGTAC102200.019.60074410-11
GTACTTT61400.019.24561114-15
ACATGGG82050.019.2287643
AGTACTT61100.018.98962612-13
CAGAGTA111700.018.8664410-11
TGGTATC24650.018.8362672
Produced by FastQC (version 0.11.5)