##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939127_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2575482 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.870842040441364 33.0 31.0 34.0 30.0 34.0 2 31.99544007684775 34.0 31.0 34.0 30.0 34.0 3 32.055197823164754 34.0 31.0 34.0 30.0 34.0 4 35.49634437359686 37.0 35.0 37.0 33.0 37.0 5 35.5353126133283 37.0 35.0 37.0 33.0 37.0 6 35.60439871061028 37.0 35.0 37.0 33.0 37.0 7 35.518259106450756 37.0 35.0 37.0 33.0 37.0 8 35.50119433954499 37.0 35.0 37.0 33.0 37.0 9 37.14950094778376 39.0 37.0 39.0 34.0 39.0 10-11 37.0552292735884 39.0 37.0 39.0 33.0 39.0 12-13 36.95796747948539 39.0 37.0 39.0 33.0 39.0 14-15 38.248531537009384 40.0 38.0 41.0 33.0 41.0 16-17 38.16833237428955 40.0 38.0 41.0 33.0 41.0 18-19 38.07318008823203 40.0 38.0 41.0 33.0 41.0 20-21 37.9581938448803 40.0 38.0 41.0 33.0 41.0 22-23 37.8677303898843 40.0 38.0 41.0 32.5 41.0 24-25 37.913489203186046 40.0 38.0 41.0 33.0 41.0 26-27 37.83004249301684 40.0 38.0 41.0 32.5 41.0 28-29 37.70372477850748 40.0 38.0 41.0 32.0 41.0 30-31 37.551053356226134 40.0 38.0 41.0 32.0 41.0 32-33 37.36496294674162 40.0 37.0 41.0 31.5 41.0 34-35 37.059996730709045 40.0 37.0 41.0 30.5 41.0 36-37 36.818482520941714 40.0 37.0 41.0 30.0 41.0 38-39 36.58247019392874 40.0 36.0 41.0 30.0 41.0 40-41 36.43817506781255 40.0 36.0 41.0 30.0 41.0 42-43 36.25283713883459 39.0 36.0 41.0 29.5 41.0 44-45 36.011712176594514 39.0 35.0 41.0 28.5 41.0 46-47 35.69831647047038 39.0 35.0 41.0 27.0 41.0 48-49 35.73699039636075 39.0 35.0 41.0 27.0 41.0 50-51 34.76825231160614 38.0 34.0 40.0 25.5 40.5 52-53 34.94280546321038 38.5 34.5 39.5 26.0 40.5 54-55 35.45816200617981 39.0 35.0 40.5 27.0 41.0 56-57 35.34662502009333 39.0 35.0 41.0 26.0 41.0 58-59 35.15729812904924 39.0 35.0 41.0 26.0 41.0 60-61 34.86837900633745 38.0 34.5 40.5 26.0 41.0 62-63 34.49798659047122 38.0 34.0 40.0 25.5 41.0 64-65 34.124993302224595 37.0 34.0 40.0 23.5 41.0 66-67 33.73298260286812 37.0 34.0 40.0 23.0 41.0 68-69 33.33229061589248 36.0 33.0 39.0 22.0 41.0 70-71 32.935876274809914 36.0 33.0 39.0 21.5 40.5 72-73 32.482112668618925 35.0 32.5 38.5 20.0 40.0 74-75 32.01994558688432 35.0 32.0 37.5 18.5 39.5 76-77 31.574969462026914 35.0 31.5 37.0 17.5 39.0 78-79 31.148158286487735 35.0 31.0 36.5 14.0 39.0 80-81 30.72654807915567 35.0 31.0 36.0 9.5 37.5 82-83 30.29979961032537 34.5 31.0 35.5 7.0 37.0 84-85 29.915905449931316 34.0 30.0 35.0 2.0 37.0 86-87 29.51905953914646 34.0 30.0 35.0 2.0 36.0 88-89 29.118391625334596 34.0 29.0 35.0 2.0 36.0 90-91 28.743934533419377 34.0 29.0 35.0 2.0 35.5 92-93 28.335689979584405 34.0 29.0 35.0 2.0 35.0 94-95 27.875144730190307 34.0 28.5 35.0 2.0 35.0 96-97 27.295278708995056 34.0 27.0 35.0 2.0 35.0 98-99 26.572302194307706 33.5 25.0 35.0 2.0 35.0 100-101 24.04700226986638 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3142648706068165 1101 2 0.32039166268047126 1101 3 0.32731756584944094 1101 4 0.2912038940729147 1101 5 0.37164475164201605 1101 6 0.4204163809375032 1101 7 0.26789661358565553 1101 8 0.47806347142885386 1101 9 0.5115190474277185 1101 10-11 0.4944289914392783 1101 12-13 0.4870422991235728 1101 14-15 0.5345143585104992 1101 16-17 0.6247868583258978 1101 18-19 0.5461293292599478 1101 20-21 0.5899161649955218 1101 22-23 0.6571548004083425 1101 24-25 0.5921821674438945 1101 26-27 0.6703724976235534 1101 28-29 0.5687835118183315 1101 30-31 0.4873831505550541 1101 32-33 0.49706815974731455 1101 34-35 0.4523194241135471 1101 36-37 0.5470272312413016 1101 38-39 0.6008848156461326 1101 40-41 0.45753407033266313 1101 42-43 0.4126819916323612 1101 44-45 0.33213868491708354 1101 46-47 0.46499234319764327 1101 48-49 0.48124478378419866 1101 50-51 0.43824555286607847 1101 52-53 0.5015593353439343 1101 54-55 0.4659178057300295 1101 56-57 0.45400885583429584 1101 58-59 0.34591949387579746 1101 60-61 0.3493861187672067 1101 62-63 0.4467496281592318 1101 64-65 0.33650531857731636 1101 66-67 0.21849939486822478 1101 68-69 0.3214746264598247 1101 70-71 0.30547869966402175 1101 72-73 0.2063467204812568 1101 74-75 0.15197785138937547 1101 76-77 -0.006077131214862419 1101 78-79 0.1496391351869235 1101 80-81 0.1547461090684905 1101 82-83 0.21945323340996126 1101 84-85 0.10760375487509322 1101 86-87 0.08271991413880997 1101 88-89 0.14372994722160826 1101 90-91 -0.02189890466648947 1101 92-93 -0.24662938531429646 1101 94-95 -0.45994538705658883 1101 96-97 -0.3682934289961217 1101 98-99 -0.29055610907013474 1101 100-101 -0.5009830285045886 1102 1 0.147535960482589 1102 2 0.21406582424154408 1102 3 0.3451822854625419 1102 4 0.3308294478604523 1102 5 0.34021930085925334 1102 6 0.31207854717407457 1102 7 0.345673626627061 1102 8 0.3632592919533977 1102 9 0.5188238222895478 1102 10-11 0.31443596792063744 1102 12-13 0.3176937867291727 1102 14-15 0.48773849791811585 1102 16-17 0.5305808964845866 1102 18-19 0.44169191169994093 1102 20-21 0.511608290213978 1102 22-23 0.45582230811121605 1102 24-25 0.35515777752428335 1102 26-27 0.38697581935156933 1102 28-29 0.48155285816034876 1102 30-31 0.46646123435339604 1102 32-33 0.4962832078298973 1102 34-35 0.3170359087490624 1102 36-37 0.3990658967467908 1102 38-39 0.4239514328308758 1102 40-41 0.4226766912762585 1102 42-43 0.4370641878396455 1102 44-45 0.24394182378620854 1102 46-47 0.2830526311026418 1102 48-49 0.35337456197979833 1102 50-51 0.26476423783063296 1102 52-53 0.23242054755449004 1102 54-55 0.29303911164599583 1102 56-57 0.3578753672768613 1102 58-59 0.266193054849424 1102 60-61 0.2400101227904159 1102 62-63 0.16425570813927237 1102 64-65 0.23337296056963197 1102 66-67 0.3063153651446626 1102 68-69 0.17169008006992925 1102 70-71 0.15866218855385483 1102 72-73 0.1539442142663674 1102 74-75 0.12637545529651817 1102 76-77 0.150161833606699 1102 78-79 0.03692174094963718 1102 80-81 -0.03887911153731238 1102 82-83 -0.013789817580530439 1102 84-85 0.07159646867550506 1102 86-87 -0.08498497991761411 1102 88-89 0.05079668485233668 1102 90-91 -0.045625882571300735 1102 92-93 -0.20886448726125906 1102 94-95 -0.277054327706832 1102 96-97 -0.33275511954929016 1102 98-99 -0.2921568087517734 1102 100-101 -0.5031214334478804 1103 1 0.3286101426810042 1103 2 0.3451177761938382 1103 3 0.3724991004510798 1103 4 0.3610230958950993 1103 5 0.40064608361678467 1103 6 0.37777357553424906 1103 7 0.34928191260484454 1103 8 0.34282644950793895 1103 9 0.5181766582500771 1103 10-11 0.5914020537912776 1103 12-13 0.5086336610324125 1103 14-15 0.6521988458793686 1103 16-17 0.6190568963615704 1103 18-19 0.6341576237759128 1103 20-21 0.6711001496914903 1103 22-23 0.5739607841300725 1103 24-25 0.6805918748111282 1103 26-27 0.6248946962286581 1103 28-29 0.5860568465057057 1103 30-31 0.660646420846362 1103 32-33 0.6424485947861527 1103 34-35 0.47976700554118423 1103 36-37 0.4096953145573323 1103 38-39 0.3682199590168196 1103 40-41 0.32085343141474 1103 42-43 0.4260864139092959 1103 44-45 0.36546087288600404 1103 46-47 0.3567556737418087 1103 48-49 0.3437158902284665 1103 50-51 0.41791544640804545 1103 52-53 0.3005021475551004 1103 54-55 0.2993641956786064 1103 56-57 0.2218329054185375 1103 58-59 0.2855381149698033 1103 60-61 0.4060906589285196 1103 62-63 0.30494127298305074 1103 64-65 0.16997565880135568 1103 66-67 0.10822327459408854 1103 68-69 -0.09725944872858605 1103 70-71 0.083837287337019 1103 72-73 -0.030000821022966306 1103 74-75 0.009733676633835842 1103 76-77 -0.010158107284770068 1103 78-79 0.03351933628626114 1103 80-81 -0.038241344570280944 1103 82-83 0.005079503882495118 1103 84-85 0.06789212373498188 1103 86-87 -0.09851059443868948 1103 88-89 -0.10232433918936223 1103 90-91 -0.30376507618478144 1103 92-93 -0.2556666540173431 1103 94-95 -0.18888786170088423 1103 96-97 -0.29392537210083347 1103 98-99 -0.5206276411881134 1103 100-101 -0.5081929100487912 1104 1 0.2852161803524318 1104 2 0.2760199689180354 1104 3 0.2940951692172291 1104 4 0.18847703150581196 1104 5 0.18702334483472782 1104 6 0.3052335463153213 1104 7 0.20965960552776153 1104 8 0.3626805288753232 1104 9 0.4377430611634878 1104 10-11 0.4243539752318384 1104 12-13 0.3977993884417117 1104 14-15 0.5368104663552344 1104 16-17 0.5162961593313398 1104 18-19 0.5633107045129009 1104 20-21 0.44816965582425183 1104 22-23 0.4630256535121333 1104 24-25 0.5688523266659615 1104 26-27 0.5520159362767529 1104 28-29 0.6020560287673007 1104 30-31 0.46081350263488474 1104 32-33 0.35254673970527506 1104 34-35 0.5035398678267526 1104 36-37 0.42003149529492134 1104 38-39 0.5620685436606223 1104 40-41 0.39780810619789975 1104 42-43 0.5301297605689328 1104 44-45 0.537749491044039 1104 46-47 0.5046358337445085 1104 48-49 0.5368340017747215 1104 50-51 0.3576151232817608 1104 52-53 0.46782425547156237 1104 54-55 0.5176051990610233 1104 56-57 0.4870036110278164 1104 58-59 0.3866285778238705 1104 60-61 0.40854414597927047 1104 62-63 0.3514425324850521 1104 64-65 0.2942591182480001 1104 66-67 0.3357739360433314 1104 68-69 0.32011988456451235 1104 70-71 0.34317589791031367 1104 72-73 0.2672277852495881 1104 74-75 0.2419006997017803 1104 76-77 0.16410607767634744 1104 78-79 0.10314698802321942 1104 80-81 0.19510117744585642 1104 82-83 -0.058489869857115906 1104 84-85 0.04400807295038334 1104 86-87 -0.05206397769538995 1104 88-89 0.12222450464054546 1104 90-91 0.11103109545111067 1104 92-93 -0.08251054808324554 1104 94-95 -0.061943218349547635 1104 96-97 0.057556969045055695 1104 98-99 -0.21707216465160428 1104 100-101 -0.28994429473979366 1105 1 0.12986111180872584 1105 2 0.14209293263117928 1105 3 0.120733487432787 1105 4 0.17228255945377668 1105 5 0.1503606410802334 1105 6 0.16326536283877857 1105 7 0.16756781693339917 1105 8 0.23697810793436958 1105 9 0.22763375607795666 1105 10-11 0.21662818359295244 1105 12-13 0.31735829954247663 1105 14-15 0.34645281877345013 1105 16-17 0.2042763217058976 1105 18-19 0.33992197428028703 1105 20-21 0.3050817924000455 1105 22-23 0.31273876173528237 1105 24-25 0.17625012966099973 1105 26-27 0.20792193348383847 1105 28-29 0.39203085693626605 1105 30-31 0.21887352083492573 1105 32-33 0.2811506309076037 1105 34-35 0.22106259691033614 1105 36-37 0.24479267217499512 1105 38-39 0.3330827241765135 1105 40-41 0.3467151077621011 1105 42-43 0.15330608709611937 1105 44-45 0.3504510439185111 1105 46-47 0.3619746648321893 1105 48-49 0.3823454271231057 1105 50-51 0.3012589476387859 1105 52-53 0.23503068545381467 1105 54-55 0.3261661377470091 1105 56-57 0.36967598722350914 1105 58-59 0.32849721672079824 1105 60-61 0.39703990629228514 1105 62-63 0.38259673589777066 1105 64-65 0.3165342491918395 1105 66-67 0.3464486375668443 1105 68-69 0.488216292362857 1105 70-71 0.29284901007540043 1105 72-73 0.34208279298670874 1105 74-75 0.35865301978765984 1105 76-77 0.4410431344697052 1105 78-79 0.18470723267538602 1105 80-81 0.049048730714524424 1105 82-83 -0.021866218610163912 1105 84-85 -0.0032360256653234387 1105 86-87 0.06081559171009232 1105 88-89 0.09121602736333756 1105 90-91 0.05296342400798437 1105 92-93 -0.05084892416990172 1105 94-95 -0.2873422353795867 1105 96-97 -0.09576920378823317 1105 98-99 -0.1109153926846993 1105 100-101 -0.0025068311713276614 1106 1 0.2206468845251912 1106 2 0.2703519581674314 1106 3 0.20987866568687252 1106 4 0.3529212277162088 1106 5 0.39298778841035187 1106 6 0.2710031054775115 1106 7 0.3487766887363293 1106 8 0.2668709781737775 1106 9 0.3616774260127187 1106 10-11 0.2780106954705204 1106 12-13 0.2663061934161135 1106 14-15 0.39450254132813 1106 16-17 0.4116533551967976 1106 18-19 0.4728438745289125 1106 20-21 0.4980458232353655 1106 22-23 0.4976118964846492 1106 24-25 0.481330431647244 1106 26-27 0.5128337433461425 1106 28-29 0.2817712732855995 1106 30-31 0.4080914353003635 1106 32-33 0.3269046797557138 1106 34-35 0.35528130724291174 1106 36-37 0.348042328642471 1106 38-39 0.4448917301686919 1106 40-41 0.461451695889771 1106 42-43 0.39382418652511575 1106 44-45 0.43663186896072403 1106 46-47 0.3533878629285496 1106 48-49 0.4217620061436378 1106 50-51 0.4228624083511576 1106 52-53 0.40682590984805245 1106 54-55 0.27467353427420704 1106 56-57 0.3513974881281783 1106 58-59 0.3904924959190481 1106 60-61 0.48523734783130834 1106 62-63 0.46365001063916367 1106 64-65 0.3794450274491581 1106 66-67 0.3913879300308949 1106 68-69 0.4420824436299995 1106 70-71 0.4183884871000316 1106 72-73 0.5065411802173685 1106 74-75 0.3708373426155518 1106 76-77 0.30351250869650315 1106 78-79 0.3965451518152854 1106 80-81 0.36330870524678005 1106 82-83 0.3475962084017681 1106 84-85 0.5143914238928211 1106 86-87 0.41524695975531856 1106 88-89 0.4873374847471723 1106 90-91 0.5381839698720938 1106 92-93 0.5347720234559965 1106 94-95 0.2936442973396183 1106 96-97 0.3952276752036923 1106 98-99 0.4830241294726427 1106 100-101 0.3196153535746227 1107 1 0.12310867239107992 1107 2 0.09335882218756097 1107 3 0.2114826105960077 1107 4 0.2618542729580611 1107 5 0.21929484149665512 1107 6 0.23637075842055566 1107 7 0.149723541552909 1107 8 0.26601573727028693 1107 9 0.4089493695452191 1107 10-11 0.31114342522568705 1107 12-13 0.3123637635991372 1107 14-15 0.4711273640325615 1107 16-17 0.4219366206067221 1107 18-19 0.345181004405255 1107 20-21 0.30514996869897715 1107 22-23 0.4473075789909515 1107 24-25 0.3944110516036119 1107 26-27 0.38829751024537273 1107 28-29 0.29859722979792735 1107 30-31 0.3078480696770214 1107 32-33 0.39732826947966515 1107 34-35 0.40645510329004964 1107 36-37 0.41819883969162674 1107 38-39 0.5087419441998478 1107 40-41 0.39813023872030584 1107 42-43 0.41821131706422676 1107 44-45 0.5652835193252841 1107 46-47 0.7468467919725228 1107 48-49 0.6118397878981412 1107 50-51 0.5752609859608384 1107 52-53 0.6650163634546757 1107 54-55 0.6948884442358008 1107 56-57 0.6748587295628781 1107 58-59 0.6140830053961679 1107 60-61 0.4727690941085285 1107 62-63 0.5213402317793268 1107 64-65 0.6211549102929368 1107 66-67 0.595826591043739 1107 68-69 0.6853918528018923 1107 70-71 0.6831498616322165 1107 72-73 0.6001176435163913 1107 74-75 0.6594782018233545 1107 76-77 0.7717019165722796 1107 78-79 0.7410255831473407 1107 80-81 0.7563950006249911 1107 82-83 0.8080366525575329 1107 84-85 0.7856488489500038 1107 86-87 0.8036819621830844 1107 88-89 0.7803149357463361 1107 90-91 0.7342670941819947 1107 92-93 0.6784446399874788 1107 94-95 0.6227232735838548 1107 96-97 0.7500277025007058 1107 98-99 0.8296298982738293 1107 100-101 0.8819482759799193 1108 1 0.02890940518054208 1108 2 0.03560487735643392 1108 3 0.10047105588954963 1108 4 0.06405784376318735 1108 5 0.10371518347133701 1108 6 0.1698438363525412 1108 7 0.21578208886722194 1108 8 0.23660543816544788 1108 9 0.12562416891503148 1108 10-11 0.2264417136599164 1108 12-13 0.32226779770196146 1108 14-15 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-1.9161152276026243 2316 98-99 -1.988329494320002 2316 100-101 -1.8082324084194816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11194.0 3 3168.0 4 3246.0 5 3478.0 6 3900.0 7 4471.0 8 5286.0 9 6402.0 10 7512.0 11 8868.0 12 10748.0 13 14764.0 14 12912.0 15 12622.0 16 14260.0 17 17785.0 18 17209.0 19 13335.0 20 13857.0 21 14978.0 22 16100.0 23 18549.0 24 20510.0 25 23341.0 26 26771.0 27 31320.0 28 36230.0 29 43190.0 30 51779.0 31 63155.0 32 78966.0 33 103310.0 34 142119.0 35 209537.0 36 337581.0 37 544616.0 38 535585.0 39 92788.0 40 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.08643772235893 19.071129922284435 15.318903411100843 23.523528944255794 2 15.215369891364602 19.85925523371033 40.555919669048045 24.369455205877024 3 19.06195484185944 23.846130523880454 29.42372994673193 27.66818468752818 4 13.317060920864607 16.84614523419679 36.77646852108417 33.06032532385444 5 14.683457981278703 36.37927655981002 33.98184478959147 14.95542066931981 6 32.30883660488338 35.377935534816054 17.793289229066538 14.519938631234028 7 28.841893025770215 31.84486183327653 20.87259389208371 18.44065124886955 8 25.894979857633405 35.28192511664694 19.937308948729758 18.885786076989895 9 25.956462213869646 17.50663263977703 20.47147216817273 36.06543297818059 10-11 25.060253652136876 26.455472531640456 27.20441707263237 21.2798567435903 12-13 25.687343566952325 23.598531858663126 27.694602662730688 23.01952191165386 14-15 22.881814219555526 25.233163612000048 25.663124754798567 26.221897413645863 16-17 22.644832219772397 28.14368860810453 26.402294062467696 22.809185109655377 18-19 22.603197847840086 27.132834546488343 28.225101562624488 22.038866043047083 20-21 23.346073089639013 26.344476376970334 28.148823881970753 22.1606266514199 22-23 23.080730995351157 26.14723111031381 27.947859235952155 22.824178658382877 24-25 22.81112485192769 26.375201528016817 28.134516330815796 22.679157289239697 26-27 22.769723305637008 26.577606893595764 27.97141016058945 22.681259640177785 28-29 22.74732411005655 26.608823735790576 28.075643128113253 22.56820902603962 30-31 22.90424660627944 26.454072678465018 27.897990335918376 22.743690379337167 32-33 22.817573336085204 26.448681988133142 27.879887849617273 22.85385682616438 34-35 23.07371530866424 26.53076806362702 27.74202495397991 22.653491673728826 36-37 23.14144646038062 26.35460200948539 27.77411344425437 22.72983808587962 38-39 22.830262424969614 26.602397071650017 27.80976803186734 22.757572471513026 40-41 23.063052909252292 26.406715795089546 27.717318393051304 22.812912902606854 42-43 23.010965822967275 26.389150859209554 27.609515351547483 22.99036796627569 44-45 22.964710206006846 26.564421737509665 27.559665362498276 22.911202693985214 46-47 23.104959495905696 26.6823123180242 27.30952346116143 22.903204724908676 48-49 23.148894060887766 26.58347158100644 27.335930432040968 22.931703926064824 50-51 23.089258239126785 26.785275003662655 27.230880487329546 22.894586269881014 52-53 23.147812342526763 26.791466269830046 27.042272425505832 23.018448962137363 54-55 23.007366559739552 26.716063157545676 27.11071491618624 23.16585536652853 56-57 22.93534987128481 26.87705749278415 27.13415633044699 23.053436305484045 58-59 23.022765268195894 26.646520825753296 27.326004240531827 23.004709665518984 60-61 23.097731599652597 26.606341791237938 27.39226345751739 22.903663151592074 62-63 22.870468126139897 26.669398554855857 27.384734570276652 23.075398748727594 64-65 22.898936403712003 26.504784378606335 27.34604290088742 23.250236316794247 66-67 22.888608006905585 26.68484638231333 27.18586821084984 23.24067739993124 68-69 22.826326641404926 26.62905245837531 27.317531801263033 23.227089098956732 70-71 22.87080006501627 26.480549461635228 27.280686674845082 23.367963798503418 72-73 22.869759784473693 26.58260547548892 27.207796439398642 23.339838300638753 74-75 22.832038852948262 26.746328999319996 27.016906456922147 23.404725690809595 76-77 22.899792728425467 26.630310419663978 27.096549537906313 23.373347314004242 78-79 22.771234635274144 26.55026187956466 27.19480898414446 23.483694501016743 80-81 22.79935198704598 26.675376014860763 27.10392587717947 23.42134612091379 82-83 22.883084770242185 26.618885228965834 27.105679270318067 23.39235073047391 84-85 22.880691380912587 26.48422802898544 27.15459629262903 23.48048429747294 86-87 22.817781491976433 26.422660981445784 27.289664969137426 23.469892557440357 88-89 22.8366610069 26.34314105929527 27.247103929977314 23.573094003827418 90-91 22.714194757043956 26.44173291079146 27.30825464983706 23.535817682327526 92-93 22.768591340635354 26.27031389109095 27.298010301548274 23.66308446672542 94-95 22.70858894269043 26.255510235301095 27.339059763418906 23.69684105858957 96-97 22.767742509516033 26.1310749817172 27.34829569977655 23.75288680899022 98-99 22.63992610843205 26.09100884466772 27.273699268810624 23.995365778089607 100-101 22.897525991650756 25.621730742631694 27.574082032816015 23.906661232901538 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2046.0 1 1146.0 2 234.5 3 232.5 4 214.5 5 221.0 6 363.0 7 667.0 8 1082.5 9 1435.0 10 1512.5 11 1535.0 12 1774.5 13 2012.0 14 2244.0 15 2467.0 16 2622.5 17 2809.5 18 2960.0 19 3095.5 20 3287.5 21 3598.5 22 4260.5 23 5240.5 24 6444.5 25 8181.0 26 10710.0 27 13969.5 28 17330.0 29 21096.5 30 25273.0 31 29931.5 32 34775.0 33 39009.0 34 43845.5 35 49069.0 36 55252.5 37 61473.5 38 66023.5 39 69963.5 40 74112.0 41 79390.5 42 84128.5 43 87206.5 44 91010.5 45 95523.5 46 97940.5 47 99293.5 48 101239.5 49 102017.0 50 100474.5 51 99049.0 52 97909.5 53 95689.5 54 93188.5 55 88043.0 56 79636.5 57 70861.0 58 64217.5 59 56719.5 60 47727.5 61 39502.0 62 31619.5 63 25094.5 64 19033.5 65 13600.5 66 10114.0 67 7991.0 68 6165.5 69 4639.0 70 3550.5 71 2782.0 72 2195.0 73 1685.5 74 1236.5 75 843.0 76 594.0 77 356.5 78 190.0 79 131.0 80 91.0 81 62.0 82 41.5 83 33.0 84 28.5 85 20.0 86 17.0 87 15.5 88 11.5 89 12.0 90 12.0 91 10.0 92 9.0 93 7.0 94 6.0 95 5.0 96 5.0 97 3.5 98 2.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33725725902957193 2 0.11741491495572479 3 0.1100376550874749 4 0.11764788105682743 5 0.13348957593180616 6 0.084877316168391 7 0.09299230202346591 8 0.09889410991806581 9 0.08631394045852388 10-11 0.08518793763652784 12-13 0.08916777519703108 14-15 0.09095384863881789 16-17 0.07982971731116739 18-19 0.0947007200982185 20-21 0.10467943476211443 22-23 0.11289148982598209 24-25 0.1265394205822444 26-27 0.12578228075366088 28-29 0.12088999263050568 30-31 0.11116365790947093 32-33 0.10431057176870193 34-35 0.3260166446513701 36-37 0.48486069791984565 38-39 0.4602633604117598 40-41 0.43945172204659166 42-43 0.47014500586686303 44-45 0.477211644266976 46-47 0.48416179961653777 48-49 0.4950723786848442 50-51 0.483501728996747 52-53 0.4871515312473549 54-55 0.47406660190209055 56-57 0.4535849988468178 58-59 0.4341323294047483 60-61 0.3941009876986133 62-63 0.46327250588433544 64-65 0.49367458207822845 66-67 0.5016148433574763 68-69 0.5141173574499841 70-71 0.5065653730059072 72-73 0.49796504110686857 74-75 0.5047404718806033 76-77 0.4822204154406826 78-79 0.46201060617002954 80-81 0.46410730107995324 82-83 0.47692043664059774 84-85 0.4761827106537728 86-87 0.47932775301865826 88-89 0.4913837487507193 90-91 0.5068371667905269 92-93 0.49922694082117447 94-95 0.5039056766849855 96-97 0.5033620891157461 98-99 0.4973049704870777 100-101 0.4851713193879825 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2575482.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.674509126267026 #Duplication Level Percentage of deduplicated Percentage of total 1 74.79227378620824 43.88399950843137 2 14.031157343365683 16.465425391907967 3 5.007523097694758 8.81441879087052 4 2.257759213845155 5.298916543906841 5 1.1518500766198874 3.379211891636248 6 0.700679599687451 2.466721893987028 7 0.43390385719236524 1.7821367080129131 8 0.31802599571867324 1.492801535054836 9 0.22054200922198847 1.1646174719538744 >10 1.0127326932851908 10.377159772654034 >50 0.05005750156375133 2.003181420031804 >100 0.022765420950131753 2.19019023505646 >500 4.641665933754026E-4 0.2013036872510918 >1k 2.6523805335011127E-4 0.4799151492450938 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4811 0.18679998540079099 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3961 0.15379645441125195 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.882768351710476E-5 3 0.0 0.0 0.0 0.0 3.882768351710476E-5 4 0.0 0.0 0.0 0.0 3.882768351710476E-5 5 0.0 0.0 0.0 0.0 3.882768351710476E-5 6 0.0 0.0 0.0 3.882768351710476E-5 3.882768351710476E-5 7 0.0 0.0 0.0 3.882768351710476E-5 3.882768351710476E-5 8 0.0 0.0 0.0 3.882768351710476E-5 3.882768351710476E-5 9 0.0 0.0 0.0 7.765536703420952E-5 3.882768351710476E-5 10-11 0.0 0.0 0.0 1.1648305055131428E-4 3.882768351710476E-5 12-13 0.0 0.0 0.0 3.1062146813683807E-4 3.882768351710476E-5 14-15 3.882768351710476E-5 0.0 0.0 3.1062146813683807E-4 3.882768351710476E-5 16-17 3.882768351710476E-5 0.0 0.0 3.8827683517104756E-4 3.882768351710476E-5 18-19 3.882768351710476E-5 0.0 0.0 5.047598857223619E-4 3.882768351710476E-5 20-21 3.882768351710476E-5 0.0 0.0 5.241737274809142E-4 5.824152527565714E-5 22-23 3.882768351710476E-5 0.0 0.0 7.959675121006475E-4 7.765536703420952E-5 24-25 3.882768351710476E-5 0.0 0.0 0.0010095197714447236 7.765536703420952E-5 26-27 3.882768351710476E-5 0.0 0.0 0.0012424858725473523 7.765536703420952E-5 28-29 3.882768351710476E-5 0.0 0.0 0.002310247169267733 7.765536703420952E-5 30-31 3.882768351710476E-5 0.0 0.0 0.005960049419875581 7.765536703420952E-5 32-33 3.882768351710476E-5 0.0 0.0 0.013686758439779427 7.765536703420952E-5 34-35 3.882768351710476E-5 0.0 0.0 0.02508268355204967 7.765536703420952E-5 36-37 3.882768351710476E-5 0.0 0.0 0.0460690464930448 7.765536703420952E-5 38-39 3.882768351710476E-5 0.0 0.0 0.07717002099024571 7.765536703420952E-5 40-41 3.882768351710476E-5 0.0 0.0 0.11227024688970841 7.765536703420952E-5 42-43 3.882768351710476E-5 0.0 0.0 0.148807097079304 7.765536703420952E-5 44-45 3.882768351710476E-5 0.0 0.0 0.19505086814817577 7.765536703420952E-5 46-47 3.882768351710476E-5 0.0 0.0 0.2521275629183197 9.706920879276189E-5 48-49 3.882768351710476E-5 0.0 0.0 0.3190082477765327 1.5531073406841904E-4 50-51 3.882768351710476E-5 0.0 0.0 0.3869372800897075 1.5531073406841904E-4 52-53 3.882768351710476E-5 0.0 0.0 0.4576230779325967 1.5531073406841904E-4 54-55 3.882768351710476E-5 0.0 0.0 0.5414326328042673 1.5531073406841904E-4 56-57 3.882768351710476E-5 0.0 0.0 0.6404820534564015 1.747245758269714E-4 58-59 3.882768351710476E-5 0.0 0.0 0.7513350898977357 1.941384175855238E-4 60-61 3.882768351710476E-5 0.0 0.0 0.8743023635964065 1.941384175855238E-4 62-63 3.882768351710476E-5 3.882768351710476E-5 0.0 0.9993469183632423 1.941384175855238E-4 64-65 3.882768351710476E-5 3.882768351710476E-5 0.0 1.1401166849545055 1.941384175855238E-4 66-67 7.765536703420952E-5 1.1648305055131428E-4 0.0 1.3022805051636936 1.941384175855238E-4 68-69 7.765536703420952E-5 1.1648305055131428E-4 0.0 1.494574607782155 2.1355225934407618E-4 70-71 9.706920879276189E-5 1.1648305055131428E-4 0.0 1.7029045436931805 2.3296610110262855E-4 72-73 1.1648305055131428E-4 1.1648305055131428E-4 0.0 1.9194271208263152 2.3296610110262855E-4 74-75 1.1648305055131428E-4 1.1648305055131428E-4 0.0 2.1444335468079374 2.3296610110262855E-4 76-77 1.1648305055131428E-4 1.3589689230986666E-4 0.0 2.3963669713086717 2.3296610110262855E-4 78-79 1.1648305055131428E-4 1.5531073406841904E-4 0.0 2.6781977121175764 2.3296610110262855E-4 80-81 1.1648305055131428E-4 1.5531073406841904E-4 0.0 2.9935561576435012 2.717937846197333E-4 82-83 1.1648305055131428E-4 1.5531073406841904E-4 0.0 3.324911608778473 2.717937846197333E-4 84-85 1.1648305055131428E-4 1.5531073406841904E-4 0.0 3.672807653091732 2.717937846197333E-4 86-87 1.1648305055131428E-4 1.5531073406841904E-4 0.0 4.041825957238296 2.717937846197333E-4 88-89 1.1648305055131428E-4 1.5531073406841904E-4 0.0 4.445284416664531 2.717937846197333E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4430 0.0 43.189972 1 GTATCAA 7200 0.0 36.332954 1 ATCAACG 8630 0.0 30.030478 3 TCAACGC 8790 0.0 29.537746 4 CAACGCA 8830 0.0 29.350285 5 TATCAAC 8890 0.0 29.20549 2 AACGCAG 9055 0.0 28.609858 6 ACGCAGA 10360 0.0 24.731724 7 CGCAGAG 10520 0.0 24.085457 8 GCAGAGT 11810 0.0 21.292553 9 GTACATG 8290 0.0 21.075356 1 GTGGTAT 2405 0.0 20.727924 1 GAGTACT 5765 0.0 20.659996 12-13 TACATGG 8205 0.0 20.094925 2 AGAGTAC 10220 0.0 19.600744 10-11 GTACTTT 6140 0.0 19.245611 14-15 ACATGGG 8205 0.0 19.228764 3 AGTACTT 6110 0.0 18.989626 12-13 CAGAGTA 11170 0.0 18.86644 10-11 TGGTATC 2465 0.0 18.836267 2 >>END_MODULE