FastQCFastQC Report
Fri 27 May 2016
SRR939127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2575482
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49020.19033330460084755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42250.1640469628597676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42300.044.8009261
GTATCAA70000.036.527551
ATCAACG85100.029.3853193
TATCAAC86700.028.952492
TCAACGC87100.028.7105714
CAACGCA88350.028.3043635
AACGCAG91650.027.4923216
ACGCAGA104050.023.9879467
CGCAGAG105350.023.6018528
GTACATG77450.021.763371
TACATGG76350.021.1309592
GCAGAGT119700.020.3763689
ACATGGG77400.020.1699243
ATACGTC5500.019.8433285
GTGGTAT23800.019.4061361
GTACTTT64850.018.87923414-15
GAGTACT65950.018.85216112-13
AGAGTAC102700.018.78299110-11
TGGTATC23850.018.7020322
AGTACTT66850.018.49187512-13
Produced by FastQC (version 0.11.5)