##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939127_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2575482 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49693533094 34.0 31.0 34.0 31.0 34.0 2 32.71404692403208 34.0 31.0 34.0 31.0 34.0 3 32.80854768156019 34.0 31.0 34.0 31.0 34.0 4 36.26824648745361 37.0 37.0 37.0 35.0 37.0 5 36.224118436859584 37.0 37.0 37.0 35.0 37.0 6 36.251760253032245 37.0 37.0 37.0 35.0 37.0 7 36.204099271515005 37.0 37.0 37.0 35.0 37.0 8 36.18826495389989 37.0 37.0 37.0 35.0 37.0 9 37.936508583636 39.0 38.0 39.0 35.0 39.0 10-11 37.87534974035928 39.0 38.0 39.0 35.0 39.0 12-13 37.811024499491744 39.0 38.0 39.0 35.0 39.0 14-15 39.19951663416789 40.5 39.0 41.0 36.0 41.0 16-17 39.13675595480768 40.0 38.5 41.0 36.0 41.0 18-19 39.14579329228471 40.5 38.5 41.0 36.0 41.0 20-21 39.20993390751711 41.0 39.0 41.0 36.0 41.0 22-23 39.17248014158126 41.0 39.0 41.0 36.0 41.0 24-25 39.127385281667664 41.0 39.0 41.0 36.0 41.0 26-27 39.0007319018343 40.0 39.0 41.0 35.0 41.0 28-29 38.856678478047996 40.0 38.0 41.0 35.0 41.0 30-31 38.690616940828946 40.0 38.0 41.0 35.0 41.0 32-33 38.5837171449849 40.0 38.0 41.0 34.0 41.0 34-35 38.56543047087885 40.0 38.0 41.0 34.0 41.0 36-37 38.4814809422081 40.0 38.0 41.0 34.0 41.0 38-39 38.3632019171557 40.0 38.0 41.0 34.0 41.0 40-41 38.210707743249614 40.0 38.0 41.0 34.0 41.0 42-43 38.0706153255973 40.0 38.0 41.0 33.0 41.0 44-45 37.88308576802323 40.0 38.0 41.0 33.0 41.0 46-47 37.6529127363344 40.0 37.0 41.0 33.0 41.0 48-49 37.392010893494884 40.0 37.0 41.0 32.5 41.0 50-51 37.16394678743629 40.0 37.0 41.0 32.0 41.0 52-53 36.943395255723004 40.0 36.0 41.0 31.0 41.0 54-55 36.70369876395952 39.0 36.0 41.0 31.0 41.0 56-57 36.43578522389207 39.0 35.0 41.0 30.5 41.0 58-59 36.14156340444235 39.0 35.0 40.0 30.0 41.0 60-61 35.79118141769192 38.0 35.0 40.0 29.0 41.0 62-63 35.40010879516922 38.0 34.5 40.0 28.5 41.0 64-65 34.994782530027386 37.0 34.0 40.0 28.0 41.0 66-67 34.6334288106071 37.0 34.0 39.5 28.0 41.0 68-69 34.171767847727146 36.0 34.0 39.0 26.5 40.5 70-71 33.73977550609944 36.0 33.0 39.0 26.0 40.0 72-73 33.271032179607545 35.0 33.0 38.0 26.0 40.0 74-75 32.77671383453661 35.0 32.5 37.0 25.5 39.0 76-77 30.787113247151407 33.5 29.5 35.5 21.5 37.5 78-79 31.766159887741402 34.5 31.5 36.0 23.5 38.5 80-81 31.801745847961662 35.0 32.0 36.0 24.0 37.5 82-83 31.509069952731178 35.0 32.0 35.5 23.5 37.0 84-85 31.13588213779013 34.5 31.5 35.0 22.0 37.0 86-87 30.58164355254667 34.0 31.0 35.0 19.0 36.0 88-89 30.05503862966233 34.0 30.5 35.0 16.5 36.0 90-91 29.61339741454221 34.0 30.0 35.0 9.0 35.0 92-93 29.336325782901994 34.0 30.0 35.0 4.5 35.0 94-95 29.01234448542059 34.0 29.5 35.0 2.0 35.0 96-97 28.465767766965563 34.0 29.0 35.0 2.0 35.0 98-99 27.6982040643266 34.0 29.0 35.0 2.0 35.0 100-101 24.053315068791008 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2549923980308719 1101 2 0.22853017459230074 1101 3 0.2399347038617421 1101 4 0.21104795728096093 1101 5 0.16752281432626148 1101 6 0.19498497678895177 1101 7 0.2112858008420062 1101 8 0.18146314626109472 1101 9 0.3268398296977395 1101 10-11 0.2856036322156257 1101 12-13 0.19835891038209041 1101 14-15 0.2645767853953416 1101 16-17 0.2944645051477295 1101 18-19 0.3144231590527866 1101 20-21 0.36671935985292947 1101 22-23 0.34734453278903743 1101 24-25 0.3097947269516155 1101 26-27 0.3147699332109326 1101 28-29 0.40237690577668417 1101 30-31 0.3502886900967397 1101 32-33 0.34195255203793806 1101 34-35 0.4481435431485963 1101 36-37 0.4615966046377693 1101 38-39 0.48120950044663857 1101 40-41 0.45723187828589573 1101 42-43 0.44281380002239956 1101 44-45 0.47969479832429585 1101 46-47 0.46401161307453975 1101 48-49 0.40876449889861277 1101 50-51 0.41514797725784547 1101 52-53 0.4268204342507502 1101 54-55 0.4813333213975639 1101 56-57 0.3974346369126067 1101 58-59 0.46611672233061086 1101 60-61 0.35728928958153716 1101 62-63 0.3467337143865521 1101 64-65 0.33468825106697864 1101 66-67 0.2882064703048144 1101 68-69 0.32213509922315353 1101 70-71 0.370698418353129 1101 72-73 0.3179329279644776 1101 74-75 0.31788663388990557 1101 76-77 0.45474417500279074 1101 78-79 0.4758193777594073 1101 80-81 0.5370302289991926 1101 82-83 0.6466379396329707 1101 84-85 0.47349228699117774 1101 86-87 0.5530454438232582 1101 88-89 0.7424933098905484 1101 90-91 0.7077171365971004 1101 92-93 0.6891455043003312 1101 94-95 0.6709705193177093 1101 96-97 0.6464278613193493 1101 98-99 0.5543341738830136 1101 100-101 0.1700322260952909 1102 1 0.09637501619013733 1102 2 0.047639672109667686 1102 3 0.09972698147564074 1102 4 0.08740331671639723 1102 5 0.04419062940101526 1102 6 0.09599148282708825 1102 7 0.08011739272723162 1102 8 0.05440573928312631 1102 9 0.13926711728166907 1102 10-11 0.17561905164723868 1102 12-13 0.16191862479998065 1102 14-15 0.12334175266570924 1102 16-17 0.19388008796353517 1102 18-19 0.10999944808872186 1102 20-21 0.13062881732592757 1102 22-23 0.11573825661501047 1102 24-25 0.10895291514688665 1102 26-27 0.0716309545850109 1102 28-29 0.026929192822912285 1102 30-31 0.0024339581144587896 1102 32-33 -0.037952737534922676 1102 34-35 0.06546662736340636 1102 36-37 0.14829099995007766 1102 38-39 0.12156504289739445 1102 40-41 0.13344483381512617 1102 42-43 0.19180144766160367 1102 44-45 0.16915935338003152 1102 46-47 0.08926800071975549 1102 48-49 0.004193886857073892 1102 50-51 0.05922346247329813 1102 52-53 0.10170740860474581 1102 54-55 0.04401000645830777 1102 56-57 0.10173711978131195 1102 58-59 0.08328905354297689 1102 60-61 0.16618209383375415 1102 62-63 0.10461226964486059 1102 64-65 0.027871477947705614 1102 66-67 -0.01113678626505532 1102 68-69 -0.0317448646274201 1102 70-71 0.05885713173979923 1102 72-73 -0.026224444874088704 1102 74-75 0.08094397517007224 1102 76-77 0.12583964290999816 1102 78-79 0.10993265094536042 1102 80-81 0.08345873199254683 1102 82-83 0.05807680762848477 1102 84-85 0.23882363270515938 1102 86-87 0.28129155093493097 1102 88-89 0.30479848949779154 1102 90-91 0.4208252763597997 1102 92-93 0.3524382822734218 1102 94-95 0.49343695966586054 1102 96-97 0.18057605601792304 1102 98-99 0.195262128025945 1102 100-101 -0.24697316229801913 1103 1 0.07226820317972482 1103 2 0.07461344649411927 1103 3 0.11224729908529696 1103 4 0.0582384108143188 1103 5 0.13917794620081736 1103 6 0.10078816624901776 1103 7 0.12580425038037646 1103 8 0.1371996426903337 1103 9 0.2069894453712493 1103 10-11 0.21724124832967107 1103 12-13 0.1657751263781222 1103 14-15 0.22010027524089537 1103 16-17 0.21690851572130754 1103 18-19 0.2456292930389239 1103 20-21 0.2378666893123622 1103 22-23 0.24375751700656423 1103 24-25 0.1571039889380188 1103 26-27 0.20843410471150747 1103 28-29 0.22919538701616915 1103 30-31 0.2391459281622943 1103 32-33 0.2192123878220258 1103 34-35 0.28510421170449973 1103 36-37 0.302688507125076 1103 38-39 0.29986376652816205 1103 40-41 0.4197190521590457 1103 42-43 0.3606619036037628 1103 44-45 0.34654371338624657 1103 46-47 0.2980781642895636 1103 48-49 0.28024779226139174 1103 50-51 0.33057408566057234 1103 52-53 0.33104875166092285 1103 54-55 0.3131381745531385 1103 56-57 0.36741466389155875 1103 58-59 0.43304761522517055 1103 60-61 0.2949553031788881 1103 62-63 0.1659303504855032 1103 64-65 0.24766558524290616 1103 66-67 0.2576242315650745 1103 68-69 0.1578900505654346 1103 70-71 0.1035621377026672 1103 72-73 0.19052425159059538 1103 74-75 0.2464324957443509 1103 76-77 0.2228045213992047 1103 78-79 0.2844683691227914 1103 80-81 0.19071188608669232 1103 82-83 0.1880141149173049 1103 84-85 0.23726732563693176 1103 86-87 0.2584729911283503 1103 88-89 0.3284542430309969 1103 90-91 0.3953648103181635 1103 92-93 0.3087230063544908 1103 94-95 0.19862488402910827 1103 96-97 0.0938258901928215 1103 98-99 0.13100485174935628 1103 100-101 -0.17707232704065845 1104 1 0.056606664233029846 1104 2 0.04617528877608379 1104 3 0.06629627313352415 1104 4 0.044252583134195334 1104 5 0.10150978379057563 1104 6 0.07210153349892323 1104 7 0.04994542117080414 1104 8 0.024993864041896074 1104 9 0.0952311392641434 1104 10-11 0.16831667762873792 1104 12-13 0.09893914871586418 1104 14-15 0.061530795478013545 1104 16-17 0.08476492651305989 1104 18-19 0.11267068397715008 1104 20-21 0.15312691661535638 1104 22-23 0.14771811115004851 1104 24-25 0.15298729592864646 1104 26-27 0.12800567286081588 1104 28-29 0.02308398864544614 1104 30-31 0.1663677543830957 1104 32-33 0.20406465391975814 1104 34-35 0.18671326424256307 1104 36-37 0.21116759525976647 1104 38-39 0.3076037365915454 1104 40-41 0.3827033405988729 1104 42-43 0.2711331416673133 1104 44-45 0.3407456001653628 1104 46-47 0.41796709540651733 1104 48-49 0.35785383047652175 1104 50-51 0.3248713933765046 1104 52-53 0.2966083179768404 1104 54-55 0.27209602568451174 1104 56-57 0.3740745559435581 1104 58-59 0.35563269073820436 1104 60-61 0.4524754244912472 1104 62-63 0.3702192361483796 1104 64-65 0.31382083918493464 1104 66-67 0.3604135133199975 1104 68-69 0.2814286286249228 1104 70-71 0.2864658593366869 1104 72-73 0.14034507227589899 1104 74-75 0.06046252696955179 1104 76-77 0.12548468074945163 1104 78-79 0.26774931608995445 1104 80-81 0.19582492591501222 1104 82-83 0.25628765928586716 1104 84-85 0.16216289272274054 1104 86-87 0.10786857697212326 1104 88-89 0.3427869570797313 1104 90-91 0.4189748317457358 1104 92-93 0.38738927720097394 1104 94-95 0.3340731367929166 1104 96-97 0.43843004010645004 1104 98-99 0.2565945721687335 1104 100-101 -0.037038975928041395 1105 1 0.008461999067662873 1105 2 0.022471727187401314 1105 3 0.02003294286149071 1105 4 -0.06349197467609713 1105 5 0.026806792525761125 1105 6 0.07824561995330725 1105 7 -0.001824574761442932 1105 8 0.06719049158267154 1105 9 0.06260106558333689 1105 10-11 0.0633212590906993 1105 12-13 0.10357460273139907 1105 14-15 0.08576169696714686 1105 16-17 0.11311626069349501 1105 18-19 0.18026507048059415 1105 20-21 0.1146691080009532 1105 22-23 0.17706908468451132 1105 24-25 0.10732233394325164 1105 26-27 0.08692243493091922 1105 28-29 0.06148492213142731 1105 30-31 0.09485710903647515 1105 32-33 0.13520092987263865 1105 34-35 0.19727264292503577 1105 36-37 0.18463233372853693 1105 38-39 0.17927012019305266 1105 40-41 0.031186216205362882 1105 42-43 0.08276664777523024 1105 44-45 0.21365432528183703 1105 46-47 0.26149994773876983 1105 48-49 0.24642496999199892 1105 50-51 0.22749252429518663 1105 52-53 0.3105453454152851 1105 54-55 0.2450887267970714 1105 56-57 0.3074412804813278 1105 58-59 0.20576200957860635 1105 60-61 0.2602629138500916 1105 62-63 0.3071976623899886 1105 64-65 0.3274708700075806 1105 66-67 0.3530181740402014 1105 68-69 0.3800983272339167 1105 70-71 0.2345045323272359 1105 72-73 0.18731580333883358 1105 74-75 0.06467312115476176 1105 76-77 0.19826387734763884 1105 78-79 0.3664229461594388 1105 80-81 0.18566262474207207 1105 82-83 0.12327717748800637 1105 84-85 0.37114596542645373 1105 86-87 0.41697992012677076 1105 88-89 0.5043178258591112 1105 90-91 0.5527778959784619 1105 92-93 0.49450584972226963 1105 94-95 0.11266193118696677 1105 96-97 -0.041013139281371735 1105 98-99 -0.13271201255581389 1105 100-101 -0.19378316051168198 1106 1 0.04708119981557246 1106 2 0.10281350678660317 1106 3 0.11491239417658505 1106 4 0.09034964574968285 1106 5 0.09698313250407864 1106 6 0.1469234126410015 1106 7 0.0787902174870112 1106 8 0.09748079167494694 1106 9 0.1336045029234043 1106 10-11 0.17102449736324843 1106 12-13 0.11734260041029643 1106 14-15 0.13995672269683013 1106 16-17 0.09604768112491513 1106 18-19 0.11271257347265617 1106 20-21 0.16242793608156347 1106 22-23 0.152896635388295 1106 24-25 0.17578630030325115 1106 26-27 0.21028284789529295 1106 28-29 0.21835083766070795 1106 30-31 0.22443435247510735 1106 32-33 0.2294610655931706 1106 34-35 0.11640045314195646 1106 36-37 0.14297977103637294 1106 38-39 0.2975724953023615 1106 40-41 0.1464302955583534 1106 42-43 0.20971072954603898 1106 44-45 0.16768871681623665 1106 46-47 0.1917651125336377 1106 48-49 0.16329186272523089 1106 50-51 0.1749695751138205 1106 52-53 0.21990942742788633 1106 54-55 0.18920550569107064 1106 56-57 0.3159779629041495 1106 58-59 0.22593195239706176 1106 60-61 0.22679292307812204 1106 62-63 0.3434355581575872 1106 64-65 0.3711750572761261 1106 66-67 0.37928783397322263 1106 68-69 0.1588558275260752 1106 70-71 0.20762336435696227 1106 72-73 0.08760800746767927 1106 74-75 0.10301553140312336 1106 76-77 0.18032966634411807 1106 78-79 0.2539968912828954 1106 80-81 0.1981841836745204 1106 82-83 0.27265766040809325 1106 84-85 0.2893194906544352 1106 86-87 0.258316251562114 1106 88-89 0.37147324561354367 1106 90-91 0.49130144155645894 1106 92-93 0.47178988764987295 1106 94-95 0.38888399020605746 1106 96-97 0.40113235731957175 1106 98-99 0.4088178959295412 1106 100-101 0.1691196590743047 1107 1 0.010605322567684539 1107 2 0.006804614637786699 1107 3 0.04567519345566495 1107 4 0.018379798146540338 1107 5 0.06163841199651188 1107 6 0.11142060512371899 1107 7 0.049148903165360025 1107 8 0.07669038608357681 1107 9 0.11847577247501562 1107 10-11 0.11000634828719313 1107 12-13 0.07801943865046468 1107 14-15 0.10353779604870539 1107 16-17 0.12314340182739159 1107 18-19 0.16743449007635292 1107 20-21 0.1810949680175966 1107 22-23 0.18925149261167462 1107 24-25 0.11865527513845109 1107 26-27 0.20915905545665936 1107 28-29 0.1520215542023564 1107 30-31 0.23625773884614176 1107 32-33 0.22000702055407828 1107 34-35 0.29314344834092054 1107 36-37 0.27624883856358196 1107 38-39 0.20782031387346223 1107 40-41 0.28895383554871756 1107 42-43 0.298997732584553 1107 44-45 0.13843820143785024 1107 46-47 0.29309667647074633 1107 48-49 0.27896074015526295 1107 50-51 0.29045532690837916 1107 52-53 0.37564599751910066 1107 54-55 0.3213797470329851 1107 56-57 0.3254062328873033 1107 58-59 0.24804400466592824 1107 60-61 0.23098517455625966 1107 62-63 0.3151212910013399 1107 64-65 0.38546352090114055 1107 66-67 0.34459019010790826 1107 68-69 0.23171037323948696 1107 70-71 0.3351556408639027 1107 72-73 0.3547455966800612 1107 74-75 0.36119150188307003 1107 76-77 0.4507092666392829 1107 78-79 0.5155859701216627 1107 80-81 0.6034377783129159 1107 82-83 0.48212810329228617 1107 84-85 0.5126453762239862 1107 86-87 0.5656779021083267 1107 88-89 0.5722206731402224 1107 90-91 0.6341647213316826 1107 92-93 0.5863443007567568 1107 94-95 0.5661191662349587 1107 96-97 0.6435605050553157 1107 98-99 0.655324639420602 1107 100-101 0.20465953357678401 1108 1 -0.03933072837344298 1108 2 -0.04528182330405173 1108 3 0.015366692916153113 1108 4 -0.01913076731607788 1108 5 0.02762287179541545 1108 6 0.018056076057376913 1108 7 0.021239471705555957 1108 8 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-0.3890451945798219 2316 88-89 -0.6537632613302833 2316 90-91 -0.9078592441895879 2316 92-93 -0.8428437623496343 2316 94-95 -0.44374205583462256 2316 96-97 -0.6751827007028055 2316 98-99 -0.6028337315757462 2316 100-101 -0.1250249948264468 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 13.0 5 31.0 6 76.0 7 169.0 8 457.0 9 897.0 10 1477.0 11 2467.0 12 3582.0 13 5076.0 14 6219.0 15 8560.0 16 10928.0 17 15217.0 18 15091.0 19 11184.0 20 10679.0 21 11424.0 22 12721.0 23 13887.0 24 16338.0 25 19112.0 26 22746.0 27 27601.0 28 34111.0 29 42000.0 30 51714.0 31 64263.0 32 80266.0 33 104204.0 34 144968.0 35 214389.0 36 354452.0 37 618661.0 38 590207.0 39 60282.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.518692216910836 19.54088731291906 15.673791388851333 23.266629081318772 2 15.54936124577846 20.229339595462132 40.00637550563351 24.2149236531259 3 19.127681731031316 23.89921575844832 29.69677132280482 27.27633118771554 4 13.609452521896873 17.33795848699389 36.45515674347559 32.59743224763365 5 14.591326982677415 36.57649325446654 33.99484057741425 14.837339185441792 6 32.344780510987846 35.70030774821956 17.513498444174722 14.441413296617874 7 28.85168679105503 31.95122311085847 20.74768917041548 18.449400927671014 8 25.849297335411393 35.65717019183205 19.723453706917773 18.770078765838782 9 25.75665448253958 17.56343084517772 20.412101501777144 36.26781317050556 10-11 25.08097513397492 26.65419521472097 27.026572113491763 21.238257537812338 12-13 25.669311608467854 23.676810787262344 27.668471377396543 22.985406226873263 14-15 22.811058386919292 25.3504523663179 25.6002526930172 26.238236553745608 16-17 22.59299885262101 28.30545131210844 26.37742750253841 22.72412233273214 18-19 22.516930811397636 27.250918468853598 28.24195626294418 21.99019445680459 20-21 23.305734615889374 26.421675631978793 28.185151361958656 22.08743839017318 22-23 23.003364030499924 26.222741995478906 28.047546051574034 22.726347922447136 24-25 22.824872533246772 26.3983874045783 28.145152225288108 22.63158783688682 26-27 22.759425102297676 26.635486349081976 28.00767400020851 22.59741454841184 28-29 22.6925484239455 26.6132902501357 28.115610980779522 22.578550345139277 30-31 22.875421852027227 26.509537646489406 27.93222954341647 22.68281095806689 32-33 22.790763049402017 26.462774734981647 27.93106300102272 22.81539921459362 34-35 23.027913245773483 26.59025663348774 27.77426784181135 22.607562278927425 36-37 23.14126060170598 26.384045130376105 27.83592661459067 22.63876765332725 38-39 22.874866914917394 26.61266692311173 27.870768777885246 22.641697384085635 40-41 23.055797991369392 26.420647441156053 27.7928654295159 22.730689137958652 42-43 23.01507373777323 26.475041845606267 27.63524416457414 22.874640252046362 44-45 23.032122327208317 26.58952198291502 27.564372497617377 22.813983192259283 46-47 23.09861446325788 26.732270379240404 27.336608083048336 22.83250707445338 48-49 23.202836307752282 26.63876486578519 27.365062021905985 22.793336804556542 50-51 23.10358725224531 26.82685292572533 27.21045117397488 22.85910864805448 52-53 23.125498087247898 26.91464010071919 27.036889275872145 22.92297253616077 54-55 23.027029503603597 26.755710193276443 27.104246894367733 23.113013408752224 56-57 22.950849588543036 26.8802305743158 27.142550404157355 23.026369432983806 58-59 23.090156435895764 26.704843734953602 27.25391089739408 22.95108893175655 60-61 23.09195675903206 26.70122780646331 27.32171294126418 22.885102493240446 62-63 22.899632424389562 26.767223663536587 27.3263820196448 23.006761892429044 64-65 22.89573094803496 26.584436153314208 27.269561003679755 23.25027189497108 66-67 22.923848426840205 26.752394782926082 27.10952515929626 23.21423163093745 68-69 22.79825348196986 26.740383084635067 27.216637350946066 23.244726082449006 70-71 22.888807393879553 26.609140853855873 27.17736469860102 23.324687053663556 72-73 22.891812095755277 26.66023291950788 27.106809521479864 23.341145463256975 74-75 22.89538424263885 26.754739501188517 26.87394048958603 23.475935766586602 76-77 22.86046535155849 26.696720686338292 27.068676632185806 23.374137329917414 78-79 22.8036841402275 26.66466573894028 27.096703050935904 23.434947069896314 80-81 22.80426853086465 26.78348578826018 26.989569799868445 23.42267588100672 82-83 22.843619653519216 26.702860742758354 26.989643627807382 23.46387597591505 84-85 22.804139186373657 26.56112525733047 27.04012297503924 23.594612581256634 86-87 22.73386993958127 26.499740351815515 27.175700194969355 23.590689513633862 88-89 22.754400208180844 26.505573418347012 27.0757901077063 23.664236265765847 90-91 22.665209330898897 26.609429596493662 27.09859079165452 23.626770280952922 92-93 22.64348964582164 26.427266818405254 27.11853548190203 23.810708053871082 94-95 22.53574282406167 26.4314602082251 27.13024202848244 23.90255493923079 96-97 22.57790968836125 26.34361257426765 27.149131696513507 23.929346040857595 98-99 22.425289255331933 26.361301201984354 27.00099536022741 24.212414182456303 100-101 22.663764410973073 25.81831729223582 27.403621400071753 24.114296896719353 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 135.0 1 99.5 2 85.5 3 115.5 4 104.5 5 108.0 6 197.0 7 485.5 8 846.5 9 1051.0 10 1054.5 11 1054.5 12 1201.0 13 1342.5 14 1524.5 15 1738.0 16 1855.0 17 1947.0 18 2159.0 19 2414.5 20 2713.0 21 3227.5 22 3958.5 23 4904.5 24 6161.0 25 8073.0 26 10624.0 27 13915.5 28 17184.0 29 20915.0 30 25189.5 31 29841.5 32 35055.0 33 39450.5 34 44359.0 35 49515.5 36 55311.5 37 61994.0 38 67017.5 39 70523.5 40 74577.5 41 80040.0 42 84548.0 43 88095.5 44 92204.0 45 96529.0 46 99029.5 47 100288.0 48 102538.5 49 102986.5 50 101483.5 51 100309.0 52 98866.5 53 96571.5 54 94314.0 55 89011.0 56 79791.0 57 71116.0 58 64600.0 59 56979.5 60 47539.5 61 38923.5 62 31253.0 63 24612.5 64 18400.5 65 12992.5 66 9511.0 67 7491.5 68 5681.0 69 4099.5 70 3125.5 71 2425.5 72 1869.5 73 1445.5 74 1052.5 75 742.5 76 493.0 77 240.5 78 127.0 79 86.5 80 44.0 81 23.0 82 12.0 83 7.5 84 5.5 85 4.0 86 3.0 87 2.0 88 2.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34475100194837316 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.70692087927619E-4 16-17 0.0018249011253039236 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.5237994286118095E-4 26-27 0.0012618997143059047 28-29 0.0 30-31 0.0032032838901611426 32-33 0.0 34-35 0.009823403929827505 36-37 0.027936518290556874 38-39 0.02063691378934118 40-41 0.013376136971642588 42-43 0.0093768855693808 44-45 0.002387902536301943 46-47 6.988983033078856E-4 48-49 8.153813538592E-4 50-51 3.882768351710476E-5 52-53 1.747245758269714E-4 54-55 0.0 56-57 0.0 58-59 0.004426355920949943 60-61 0.009279816360588038 62-63 0.003630388408849295 64-65 0.0015142796571670856 66-67 0.003572146883573638 68-69 0.0027955932132315425 70-71 1.941384175855238E-5 72-73 0.0 74-75 0.0 76-77 0.21564895425399982 78-79 3.8827683517104756E-4 80-81 0.002950903947299962 82-83 5.630014109980191E-4 84-85 0.0 86-87 0.10755268334238018 88-89 0.40398263315371646 90-91 0.3909947730172449 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0012618997143059047 100-101 5.435875692394665E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2575482.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.77147016259468 #Duplication Level Percentage of deduplicated Percentage of total 1 73.0611566209862 41.47789273152975 2 14.980180030770565 17.008936872943757 3 5.282969361918173 8.997658125001182 4 2.4129108467595826 5.479379845672509 5 1.2565144859842403 3.5667087324961146 6 0.7289972650659411 2.4831747889382525 7 0.49630154384861697 1.9723037801776004 8 0.3334881060488621 1.514608804970655 9 0.24127545430669503 1.2327806029625112 >10 1.1237676748795955 11.02267751026928 >50 0.057287900458949347 2.2299081480929064 >100 0.024397397761005177 2.308476919841039 >500 4.7937986188063494E-4 0.20422988257847102 >1k 2.7393134963703774E-4 0.5012632545259738 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4902 0.19033330460084755 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4225 0.1640469628597676 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.882768351710476E-5 7 0.0 0.0 0.0 0.0 3.882768351710476E-5 8 0.0 0.0 0.0 0.0 3.882768351710476E-5 9 0.0 0.0 0.0 0.0 3.882768351710476E-5 10-11 0.0 0.0 0.0 0.0 3.882768351710476E-5 12-13 3.882768351710476E-5 0.0 0.0 7.765536703420952E-5 3.882768351710476E-5 14-15 3.882768351710476E-5 0.0 0.0 7.765536703420952E-5 1.5531073406841904E-4 16-17 3.882768351710476E-5 0.0 0.0 2.1355225934407615E-4 2.3296610110262855E-4 18-19 3.882768351710476E-5 0.0 0.0 3.882768351710476E-4 2.3296610110262855E-4 20-21 3.882768351710476E-5 0.0 0.0 4.4651836044670473E-4 2.5237994286118095E-4 22-23 3.882768351710476E-5 0.0 0.0 7.571398285835428E-4 2.717937846197333E-4 24-25 3.882768351710476E-5 0.0 0.0 9.318644044105142E-4 2.717937846197333E-4 26-27 3.882768351710476E-5 0.0 0.0 0.0012230720307887999 2.717937846197333E-4 28-29 3.882768351710476E-5 0.0 0.0 0.002252005643992076 2.717937846197333E-4 30-31 3.882768351710476E-5 0.0 0.0 0.005960049419875581 2.717937846197333E-4 32-33 3.882768351710476E-5 0.0 0.0 0.013783827648572191 2.717937846197333E-4 34-35 3.882768351710476E-5 0.0 0.0 0.025276821969635196 2.717937846197333E-4 36-37 3.882768351710476E-5 0.0 0.0 0.04649615101173295 2.912076263782857E-4 38-39 3.882768351710476E-5 1.941384175855238E-5 0.0 0.07788833313531215 3.1062146813683807E-4 40-41 3.882768351710476E-5 3.882768351710476E-5 0.0 0.11366804349632419 3.1062146813683807E-4 42-43 3.882768351710476E-5 3.882768351710476E-5 0.0 0.15065141204636645 3.1062146813683807E-4 44-45 3.882768351710476E-5 3.882768351710476E-5 0.0 0.19778821983613165 3.1062146813683807E-4 46-47 3.882768351710476E-5 3.882768351710476E-5 0.0 0.25575795132716905 3.1062146813683807E-4 48-49 7.765536703420952E-5 3.882768351710476E-5 0.0 0.32331812064693133 3.1062146813683807E-4 50-51 7.765536703420952E-5 3.882768351710476E-5 0.0 0.3921013619974824 3.1062146813683807E-4 52-53 7.765536703420952E-5 3.882768351710476E-5 0.0 0.46364136887774793 3.1062146813683807E-4 54-55 7.765536703420952E-5 3.882768351710476E-5 0.0 0.5480915805274508 3.1062146813683807E-4 56-57 7.765536703420952E-5 3.882768351710476E-5 0.0 0.6480146240587199 3.6886299341249524E-4 58-59 7.765536703420952E-5 3.882768351710476E-5 0.0 0.7593335927022592 4.2710451868815236E-4 60-61 7.765536703420952E-5 5.824152527565714E-5 0.0 0.8834851107482017 4.2710451868815236E-4 62-63 7.765536703420952E-5 7.765536703420952E-5 0.0 1.008995597717243 4.2710451868815236E-4 64-65 7.765536703420952E-5 7.765536703420952E-5 0.0 1.1509107809722607 4.2710451868815236E-4 66-67 7.765536703420952E-5 7.765536703420952E-5 0.0 1.3147830192562013 4.2710451868815236E-4 68-69 7.765536703420952E-5 7.765536703420952E-5 0.0 1.5076012956021434 4.2710451868815236E-4 70-71 7.765536703420952E-5 7.765536703420952E-5 0.0 1.716804854392304 4.2710451868815236E-4 72-73 7.765536703420952E-5 7.765536703420952E-5 0.0 1.9348222973408473 4.2710451868815236E-4 74-75 7.765536703420952E-5 7.765536703420952E-5 0.0 2.1615953829224974 4.4651836044670473E-4 76-77 7.765536703420952E-5 7.765536703420952E-5 0.0 2.415722571541948 4.659322022052571E-4 78-79 7.765536703420952E-5 7.765536703420952E-5 0.0 2.699688834944294 4.659322022052571E-4 80-81 7.765536703420952E-5 7.765536703420952E-5 0.0 3.016949836962557 4.659322022052571E-4 82-83 7.765536703420952E-5 7.765536703420952E-5 0.0 3.350945570576692 4.659322022052571E-4 84-85 7.765536703420952E-5 7.765536703420952E-5 0.0 3.7021031403053875 4.659322022052571E-4 86-87 7.765536703420952E-5 7.765536703420952E-5 0.0 4.0742859006585945 4.659322022052571E-4 88-89 7.765536703420952E-5 7.765536703420952E-5 0.0 4.481394162335439 4.659322022052571E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4230 0.0 44.800926 1 GTATCAA 7000 0.0 36.52755 1 ATCAACG 8510 0.0 29.385319 3 TATCAAC 8670 0.0 28.95249 2 TCAACGC 8710 0.0 28.710571 4 CAACGCA 8835 0.0 28.304363 5 AACGCAG 9165 0.0 27.492321 6 ACGCAGA 10405 0.0 23.987946 7 CGCAGAG 10535 0.0 23.601852 8 GTACATG 7745 0.0 21.76337 1 TACATGG 7635 0.0 21.130959 2 GCAGAGT 11970 0.0 20.376368 9 ACATGGG 7740 0.0 20.169924 3 ATACGTC 550 0.0 19.843328 5 GTGGTAT 2380 0.0 19.406136 1 GTACTTT 6485 0.0 18.879234 14-15 GAGTACT 6595 0.0 18.852161 12-13 AGAGTAC 10270 0.0 18.782991 10-11 TGGTATC 2385 0.0 18.702032 2 AGTACTT 6685 0.0 18.491875 12-13 >>END_MODULE