FastQCFastQC Report
Fri 27 May 2016
SRR939126_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939126_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2536685
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45240.17834299489294098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37780.14893453463871154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37150.045.375561
GTATCAA63900.040.499361
ATCAACG78200.032.463463
TCAACGC79500.031.9921864
CAACGCA80700.031.5158425
TATCAAC81900.031.2281762
AACGCAG83750.030.468586
ACGCAGA97450.025.942257
CGCAGAG99650.025.3220088
GTACCGT2002.1304004E-823.671096
GTACATG78100.021.8878941
GCAGAGT113050.021.6505139
GTGGTAT19750.021.157681
TACATGG78500.020.936142
ACATGGG79700.019.6700943
AGAGTAC97150.019.61423310-11
GCGTACA2201.383898E-619.3756642
GAGTACT57550.019.16758512-13
CAGAGTA104200.019.03682510-11
TGGTATC20650.018.8074782
Produced by FastQC (version 0.11.5)