##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939126_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2536685 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91398932070793 33.0 31.0 34.0 30.0 34.0 2 32.04258865408988 34.0 31.0 34.0 30.0 34.0 3 32.1039928095132 34.0 31.0 34.0 30.0 34.0 4 35.543226297313225 37.0 35.0 37.0 33.0 37.0 5 35.57144974642102 37.0 35.0 37.0 33.0 37.0 6 35.64166816139962 37.0 36.0 37.0 33.0 37.0 7 35.56426399020769 37.0 35.0 37.0 33.0 37.0 8 35.55469835631937 37.0 35.0 37.0 33.0 37.0 9 37.20419996964542 39.0 37.0 39.0 34.0 39.0 10-11 37.10017779109349 39.0 37.0 39.0 33.5 39.0 12-13 36.99392829618183 39.0 37.0 39.0 33.0 39.0 14-15 38.26719340398985 40.0 38.0 41.0 33.0 41.0 16-17 38.162163611169696 40.0 38.0 41.0 33.0 41.0 18-19 38.03112704178879 40.0 38.0 41.0 33.0 41.0 20-21 38.00168408769713 40.0 38.0 41.0 33.0 41.0 22-23 38.04182328511423 40.0 38.0 41.0 33.0 41.0 24-25 37.99123245495598 40.0 38.0 41.0 33.0 41.0 26-27 37.87193443411381 40.0 38.0 41.0 33.0 41.0 28-29 37.727137977320794 40.0 38.0 41.0 32.0 41.0 30-31 37.52952928723906 40.0 37.5 41.0 32.0 41.0 32-33 37.344478522165744 40.0 37.0 41.0 31.5 41.0 34-35 37.04442116384179 40.0 37.0 41.0 30.5 41.0 36-37 36.79416778196741 40.0 37.0 41.0 30.0 41.0 38-39 36.608744877665146 40.0 36.0 41.0 30.0 41.0 40-41 36.42480402572649 40.0 36.0 41.0 30.0 41.0 42-43 36.22969919402685 39.0 36.0 41.0 29.5 41.0 44-45 35.9759238927971 39.0 35.0 41.0 28.5 41.0 46-47 35.90301062213085 39.0 35.0 41.0 28.0 41.0 48-49 35.8182665565492 39.0 35.0 41.0 27.0 41.0 50-51 34.71707563217349 38.0 34.0 40.0 25.5 40.5 52-53 34.87466693736116 38.5 34.5 39.5 26.0 40.5 54-55 35.41168099310714 39.0 35.0 40.5 26.5 41.0 56-57 35.325935817809466 39.0 35.0 41.0 26.0 41.0 58-59 35.121976319487835 39.0 35.0 41.0 26.0 41.0 60-61 34.82500251312244 38.0 34.5 40.5 26.0 41.0 62-63 34.43564573449206 38.0 34.0 40.0 24.5 41.0 64-65 34.059373552490754 37.0 34.0 40.0 23.5 41.0 66-67 33.663132789447644 37.0 34.0 40.0 22.5 41.0 68-69 33.244804932421644 36.0 33.0 39.0 22.0 41.0 70-71 32.82559817241794 36.0 33.0 39.0 20.5 40.5 72-73 32.37102990714259 35.0 32.0 38.5 19.5 40.0 74-75 31.913202072783967 35.0 32.0 37.0 18.0 39.5 76-77 31.463362419851105 35.0 31.5 37.0 15.5 39.0 78-79 31.01933803369358 35.0 31.0 36.0 11.5 39.0 80-81 30.57033608824115 35.0 31.0 36.0 7.5 37.5 82-83 30.1656169370655 34.0 30.5 35.5 4.5 37.0 84-85 29.770677281570237 34.0 30.0 35.0 2.0 37.0 86-87 29.337813327236134 34.0 29.5 35.0 2.0 36.0 88-89 28.91056595517378 34.0 29.0 35.0 2.0 36.0 90-91 28.485670077285906 34.0 29.0 35.0 2.0 35.5 92-93 28.055658467645763 34.0 28.5 35.0 2.0 35.0 94-95 27.550757780331416 34.0 27.0 35.0 2.0 35.0 96-97 26.912418372797568 33.5 25.5 35.0 2.0 35.0 98-99 26.14558508447048 33.0 24.5 35.0 2.0 35.0 100-101 23.220624555275883 30.0 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40763966740323454 1101 2 0.4674076826648772 1101 3 0.3783164834701154 1101 4 0.3901363379681726 1101 5 0.2668769396757469 1101 6 0.34862521693436577 1101 7 0.23589500284084863 1101 8 0.3369341390540086 1101 9 0.44030441103776496 1101 10-11 0.46682760910113785 1101 12-13 0.4618992511467255 1101 14-15 0.6460304854667882 1101 16-17 0.6743870123950728 1101 18-19 0.8349790236944301 1101 20-21 0.7565037862471939 1101 22-23 0.802340403858679 1101 24-25 0.902786374881245 1101 26-27 0.7528124383109258 1101 28-29 0.7750414385799402 1101 30-31 0.7507990864178495 1101 32-33 0.7553922224114729 1101 34-35 0.7036973959479198 1101 36-37 0.6693118638351407 1101 38-39 0.669873568022723 1101 40-41 0.5457629777420507 1101 42-43 0.5631101974519552 1101 44-45 0.564807569029675 1101 46-47 0.694834433279361 1101 48-49 0.8109511140542693 1101 50-51 0.762953884521437 1101 52-53 0.7734016126947765 1101 54-55 0.6248794436719933 1101 56-57 0.6154585057768287 1101 58-59 0.5602269141783722 1101 60-61 0.47083776694599777 1101 62-63 0.49682440830805774 1101 64-65 0.5421893481648112 1101 66-67 0.5771332023990752 1101 68-69 0.457659109723771 1101 70-71 0.34968073253007503 1101 72-73 0.30970238508755443 1101 74-75 0.204818173585398 1101 76-77 0.2650020436948246 1101 78-79 0.212658478734717 1101 80-81 -0.0917547859594876 1101 82-83 -0.050153211094659866 1101 84-85 -0.07867951854231592 1101 86-87 0.05810101320015448 1101 88-89 0.21431704396801976 1101 90-91 0.10598491947906652 1101 92-93 -0.027201580968153394 1101 94-95 -0.326769002081857 1101 96-97 -0.3771922365104139 1101 98-99 -0.6018742472336562 1101 100-101 -1.023853893645967 1102 1 0.3608056974445937 1102 2 0.3384979547124658 1102 3 0.394913677302668 1102 4 0.19328389208132535 1102 5 0.3840552892319451 1102 6 0.3937459834596666 1102 7 0.4285057183775933 1102 8 0.47598054707439985 1102 9 0.4214303307586036 1102 10-11 0.5041187830415552 1102 12-13 0.5131205225413851 1102 14-15 0.5928277543734737 1102 16-17 0.6109472794516577 1102 18-19 0.5565377984645039 1102 20-21 0.5298963177921294 1102 22-23 0.5393699469514885 1102 24-25 0.5805775776641724 1102 26-27 0.6161721855334719 1102 28-29 0.5831858534573158 1102 30-31 0.6350016243917338 1102 32-33 0.6463951141868449 1102 34-35 0.6117082783532339 1102 36-37 0.7313984022404085 1102 38-39 0.7041852895361416 1102 40-41 0.7283343044391017 1102 42-43 0.518164367034224 1102 44-45 0.5423088418622868 1102 46-47 0.6469891988566161 1102 48-49 0.7728259107981614 1102 50-51 0.6375840524517429 1102 52-53 0.6357397497694492 1102 54-55 0.6893864540745298 1102 56-57 0.5815230954482047 1102 58-59 0.6305770413780323 1102 60-61 0.5524481781733428 1102 62-63 0.48868841628029713 1102 64-65 0.4555807176491058 1102 66-67 0.568600312320811 1102 68-69 0.5116535468809502 1102 70-71 0.46117846567580756 1102 72-73 0.454182680635526 1102 74-75 0.39274574293073883 1102 76-77 0.4172702355869795 1102 78-79 0.3911002055735544 1102 80-81 0.3895943497265577 1102 82-83 0.26291909332175223 1102 84-85 0.21081654006567163 1102 86-87 0.19635153508242453 1102 88-89 0.3192654367712713 1102 90-91 0.20354858410000531 1102 92-93 0.21588802477560165 1102 94-95 0.12071766557130559 1102 96-97 0.030278057791893076 1102 98-99 -0.030617984437402157 1102 100-101 -0.36154296885469606 1103 1 0.1590854028138473 1103 2 0.16648181786423777 1103 3 0.24603994531326379 1103 4 0.16417416474399005 1103 5 0.16501695816003092 1103 6 0.12808867745720676 1103 7 0.19558852673839056 1103 8 0.20994707805585477 1103 9 0.2229267044098293 1103 10-11 0.20701814580565525 1103 12-13 0.3121653753875435 1103 14-15 0.38237191667882797 1103 16-17 0.3541442410721274 1103 18-19 0.398123498212513 1103 20-21 0.3651596319033885 1103 22-23 0.3717980055222654 1103 24-25 0.397242326161944 1103 26-27 0.4881137157072786 1103 28-29 0.3988527503042576 1103 30-31 0.5181087147516266 1103 32-33 0.3884617695464101 1103 34-35 0.38700872417607 1103 36-37 0.2892707693012113 1103 38-39 0.30005123174071 1103 40-41 0.22555073850254814 1103 42-43 0.27467443035398986 1103 44-45 0.20418735514005704 1103 46-47 0.34376374738022974 1103 48-49 0.46780515419158064 1103 50-51 0.41533662481719347 1103 52-53 0.40082231179697203 1103 54-55 0.4695223008148517 1103 56-57 0.4487281618370389 1103 58-59 0.37087807274716056 1103 60-61 0.34619383705426543 1103 62-63 0.19580199610842897 1103 64-65 0.13882339226304907 1103 66-67 0.21001378069323806 1103 68-69 0.280108855564805 1103 70-71 0.2918410012124113 1103 72-73 0.20635788416334577 1103 74-75 0.043107469201981274 1103 76-77 -3.4239121407608764E-4 1103 78-79 -0.04987824228455651 1103 80-81 -0.06658397133049476 1103 82-83 -0.2365854133655887 1103 84-85 -0.07978362469494016 1103 86-87 -0.10885790876974255 1103 88-89 -0.20755217969186646 1103 90-91 -0.07606097766906927 1103 92-93 -0.32542246821399345 1103 94-95 -0.28636845415474 1103 96-97 -0.32612196320700804 1103 98-99 -0.30263301076679383 1103 100-101 -0.568187303997167 1104 1 0.0966826329409578 1104 2 0.2055252473210203 1104 3 0.2166855375475052 1104 4 0.21729699089968335 1104 5 0.20990130964299425 1104 6 0.19390971868910611 1104 7 0.2995921550821592 1104 8 0.15408165087568904 1104 9 0.2892829065852567 1104 10-11 0.3133453172784968 1104 12-13 0.40014411519023696 1104 14-15 0.3533151279606841 1104 16-17 0.4437850824684446 1104 18-19 0.43139537103867553 1104 20-21 0.4900634886984321 1104 22-23 0.4854883829016359 1104 24-25 0.4239137159994186 1104 26-27 0.3818932602276135 1104 28-29 0.5132205766180462 1104 30-31 0.47483271162128204 1104 32-33 0.4242035247478313 1104 34-35 0.4900615920695728 1104 36-37 0.4479793872105233 1104 38-39 0.2273646961370872 1104 40-41 0.3249930486271353 1104 42-43 0.3222424914429709 1104 44-45 0.38304059298233284 1104 46-47 0.6615241541657824 1104 48-49 0.4978441252913086 1104 50-51 0.497812969438705 1104 52-53 0.42547242167491817 1104 54-55 0.41444341002878815 1104 56-57 0.5386125726066524 1104 58-59 0.4625391071808522 1104 60-61 0.5272773403426996 1104 62-63 0.5855859159844741 1104 64-65 0.5534002040634647 1104 66-67 0.4091122281731572 1104 68-69 0.2637610523332867 1104 70-71 0.2590648636989741 1104 72-73 0.4067078730965008 1104 74-75 0.25078321463295694 1104 76-77 0.19396865823890508 1104 78-79 0.10742261381485463 1104 80-81 0.12049766245183591 1104 82-83 0.08164839742300245 1104 84-85 0.0965787828178648 1104 86-87 0.259026209519007 1104 88-89 0.09939503146351214 1104 90-91 0.2395712355415789 1104 92-93 0.10841440650933265 1104 94-95 -0.038589925412562565 1104 96-97 0.0031737507625102523 1104 98-99 -0.028611537137503973 1104 100-101 -0.16661996225303355 1105 1 0.09141362822165533 1105 2 0.2092184713491676 1105 3 0.22454031604979718 1105 4 0.218656987284767 1105 5 0.17178598930190248 1105 6 0.15965534824317018 1105 7 0.18431968096478357 1105 8 0.18243842753682316 1105 9 0.22487770539846252 1105 10-11 0.18461561861830944 1105 12-13 0.16636010474987728 1105 14-15 0.18808743818154738 1105 16-17 0.1968391866974386 1105 18-19 0.29414889366481844 1105 20-21 0.3103964398618686 1105 22-23 0.2724197615776376 1105 24-25 0.34419070110015326 1105 26-27 0.4579952344912641 1105 28-29 0.2572846892563092 1105 30-31 0.3333915558292304 1105 32-33 0.3511239667782391 1105 34-35 0.34125983638566026 1105 36-37 0.3419169012834189 1105 38-39 0.298165882012583 1105 40-41 0.1933182866983003 1105 42-43 0.17158186980035595 1105 44-45 0.1382982053624957 1105 46-47 0.12407686527444639 1105 48-49 0.2655714665988995 1105 50-51 0.19848433242965058 1105 52-53 0.1372726639966757 1105 54-55 0.1841354990353139 1105 56-57 0.22777614132294843 1105 58-59 0.21757002158552297 1105 60-61 0.2825298788239152 1105 62-63 0.2230186916101573 1105 64-65 0.16424201559473062 1105 66-67 0.18552972236401644 1105 68-69 0.11777645937103642 1105 70-71 -0.022786871995698732 1105 72-73 0.16850105025330464 1105 74-75 0.07300968744221947 1105 76-77 0.05325220839047162 1105 78-79 0.017383405021426768 1105 80-81 0.1759595902117681 1105 82-83 0.061631961819777814 1105 84-85 0.047412989244747905 1105 86-87 0.07619754723618755 1105 88-89 0.24125837394135274 1105 90-91 0.12806732806498644 1105 92-93 0.06938722036731804 1105 94-95 0.06451667931041172 1105 96-97 0.08134683313505775 1105 98-99 0.06485571266375345 1105 100-101 -0.24471195797640632 1106 1 0.18825358408126291 1106 2 0.19327737705484083 1106 3 0.2484110527893364 1106 4 0.2473295324890472 1106 5 0.22875211507267323 1106 6 0.12890088211005946 1106 7 0.17289791423384315 1106 8 0.08976047924330288 1106 9 0.1900496445528077 1106 10-11 0.2961722791511008 1106 12-13 0.26850189695546334 1106 14-15 0.24721197483949453 1106 16-17 0.2475606502566663 1106 18-19 0.3444767182537163 1106 20-21 0.3908978104445282 1106 22-23 0.3319456412305044 1106 24-25 0.33539417246026204 1106 26-27 0.30728837189290914 1106 28-29 0.3188589223023186 1106 30-31 0.31707443176362204 1106 32-33 0.20662599770586354 1106 34-35 0.22022997705381187 1106 36-37 0.2398686579477598 1106 38-39 0.23044567164134833 1106 40-41 0.5007514231020878 1106 42-43 0.43939559235568737 1106 44-45 0.2335047245814721 1106 46-47 0.26027081299273647 1106 48-49 0.26281984581886775 1106 50-51 0.3222505879215092 1106 52-53 0.276001602660898 1106 54-55 0.28486672102568633 1106 56-57 0.252515231690424 1106 58-59 0.3855392186940918 1106 60-61 0.43222751420196914 1106 62-63 0.3498705923764547 1106 64-65 0.3154655981275809 1106 66-67 0.237681749916554 1106 68-69 0.1827872376682791 1106 70-71 0.24264960868847396 1106 72-73 0.3068376339512966 1106 74-75 0.2438119478763987 1106 76-77 0.3533793614819132 1106 78-79 0.3177712109445707 1106 80-81 0.1914369755978953 1106 82-83 0.32741934248076987 1106 84-85 0.3251986544762673 1106 86-87 0.2366272557158986 1106 88-89 0.37272241742433465 1106 90-91 0.3112108855927751 1106 92-93 0.23567804069185883 1106 94-95 0.3688777441973734 1106 96-97 0.261334492021394 1106 98-99 0.4696363534038106 1106 100-101 0.19688108022675266 1107 1 0.0369496178067763 1107 2 0.20629499561058395 1107 3 0.1306079556857327 1107 4 0.12228649383403933 1107 5 0.1433791693811557 1107 6 0.05083296081411248 1107 7 0.07314480915234611 1107 8 0.15664674802285106 1107 9 0.19069102548479577 1107 10-11 0.05028561987448654 1107 12-13 0.10592495223809095 1107 14-15 0.04095866821112537 1107 16-17 0.04864123541441501 1107 18-19 0.12834656516207588 1107 20-21 0.14515653784398097 1107 22-23 0.13960431740346024 1107 24-25 0.1467347530832086 1107 26-27 0.18777999929992717 1107 28-29 0.20360507821561669 1107 30-31 0.23700678759461624 1107 32-33 0.20930541641109812 1107 34-35 0.24923173219115569 1107 36-37 0.11400998429413534 1107 38-39 0.23029713579539646 1107 40-41 0.21246434057704278 1107 42-43 0.2678881267236264 1107 44-45 0.4184465489165774 1107 46-47 0.39560323936513697 1107 48-49 0.32458556437545383 1107 50-51 0.22761488389308937 1107 52-53 0.2723149744560871 1107 54-55 0.23399660538816391 1107 56-57 0.16394430398696613 1107 58-59 0.2952084298900175 1107 60-61 0.21138935214148802 1107 62-63 0.29212006541535374 1107 64-65 0.1809099669919476 1107 66-67 0.17345967885447777 1107 68-69 0.1791651674459942 1107 70-71 0.12195872280599218 1107 72-73 0.14502331320624506 1107 74-75 0.12108144963574219 1107 76-77 0.10925361302920678 1107 78-79 0.29423641471685613 1107 80-81 0.1199303497444717 1107 82-83 0.10655756164619845 1107 84-85 0.2461151258944021 1107 86-87 0.29325562847683884 1107 88-89 0.16293049357545897 1107 90-91 0.18341494760202792 1107 92-93 0.11170624465729162 1107 94-95 0.08978518799519009 1107 96-97 0.15969398445918515 1107 98-99 0.15075351092108846 1107 100-101 0.03520584781801617 1108 1 0.0997869230645172 1108 2 0.04256035447791362 1108 3 0.08217708700991011 1108 4 0.04251421928733379 1108 5 0.1306292652274621 1108 6 0.21627373316105292 1108 7 0.07864491237143767 1108 8 0.19183172468461152 1108 9 0.2719348423928878 1108 10-11 0.1470939044803643 1108 12-13 0.13205211816345042 1108 14-15 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-1.879895564287331 2316 88-89 -1.9234042956072308 2316 90-91 -2.0694445667302723 2316 92-93 -2.1408280186586452 2316 94-95 -2.0462086379283413 2316 96-97 -1.999946956635842 2316 98-99 -2.4557291934671035 2316 100-101 -2.192655450271314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10609.0 3 3192.0 4 3196.0 5 3438.0 6 3687.0 7 4440.0 8 5361.0 9 6422.0 10 7469.0 11 8739.0 12 10718.0 13 15550.0 14 13244.0 15 12547.0 16 14426.0 17 18533.0 18 16690.0 19 13645.0 20 14081.0 21 15301.0 22 16516.0 23 18184.0 24 20849.0 25 23210.0 26 26659.0 27 31202.0 28 36272.0 29 43167.0 30 51505.0 31 63420.0 32 78844.0 33 103094.0 34 141656.0 35 208652.0 36 336456.0 37 536487.0 38 515297.0 39 83901.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.13213638669755 19.1119937123893 15.272387938615065 23.48348196229808 2 15.216408948168331 19.834267160831576 40.5938898200634 24.355434070936685 3 19.015850488712218 23.779245576410467 29.50747532759071 27.6974286072866 4 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22.823085525064535 24-25 22.87364661399698 26.347407238882408 28.078170663921036 22.70077548319957 26-27 22.74025647036352 26.608070126419825 27.965186993525492 22.686486409691163 28-29 22.72532563560973 26.604816536162495 28.07412544777054 22.595732380457225 30-31 22.93291639333751 26.468653050353396 27.86390920704129 22.734521349267805 32-33 22.772293635755993 26.466040188164513 27.88260078015197 22.879065395927523 34-35 23.08529967807897 26.58426500961491 27.671106949890717 22.659328362415398 36-37 23.127870670302446 26.379779463562265 27.790119633101114 22.702230233034175 38-39 22.860098748976235 26.53606326717973 27.856288976861233 22.747549006982798 40-41 23.08564386644888 26.41239958541818 27.68327945076821 22.818677097364738 42-43 23.000273203095297 26.429754610525112 27.5655385300564 23.004433656323187 44-45 22.990797308976695 26.560817689392636 27.55142557898729 22.896959422643384 46-47 23.03478512065602 26.68858395160207 27.319552904822874 22.957078022919035 48-49 23.167892919332044 26.55745845099837 27.332977729307135 22.941670900362446 50-51 23.076529839892782 26.769196261919276 27.187416037035245 22.966857861152697 52-53 23.088502113863925 26.83914681412561 27.049354269406738 23.022996802603725 54-55 23.03361369844992 26.707333213399515 27.10408564859761 23.15496743955296 56-57 22.8979442921962 26.83575332773989 27.19841577318207 23.06788660688183 58-59 23.04350900166778 26.640611213866034 27.26817212363625 23.047707660829936 60-61 23.088708836123235 26.61538257495602 27.376725869499335 22.91918271942141 62-63 22.865545697434186 26.679626375285192 27.388012527058 23.066815400222627 64-65 22.89818462693245 26.527127555648732 27.318972941203977 23.255714876214835 66-67 22.873237594418566 26.693301545873325 27.188147478753034 23.24531338095507 68-69 22.811810250783303 26.61795742265295 27.330440614970886 23.23979171159286 70-71 22.838558869458417 26.49896115576797 27.295689177964334 23.366790796809283 72-73 22.897419154752622 26.549661478950064 27.214516226518292 23.338403139779025 74-75 22.842494835043425 26.716791930281758 26.992236463354818 23.44847677132 76-77 22.86635797338655 26.60992606537717 27.10365567946566 23.42006028177062 78-79 22.812352622281047 26.58888655384371 27.16933564971576 23.429425174159487 80-81 22.81118633445145 26.685978495254115 27.08678081299048 23.416054357303956 82-83 22.830822532640806 26.633248758368595 27.11488426265747 23.421044446333134 84-85 22.901705783371497 26.460977922243096 27.147918950626064 23.489397343759347 86-87 22.823862788786183 26.398641469992228 27.259014752737276 23.51848098848431 88-89 22.85293069326155 26.338902463838494 27.21493745055512 23.59322939234484 90-91 22.758959209054687 26.44495509199186 27.273401031564415 23.52268466738904 92-93 22.7468743361036 26.225493304753417 27.300866751434015 23.726765607708966 94-95 22.71349118181058 26.21629344740298 27.33408847795423 23.736126892832214 96-97 22.777442543683645 26.1235478005836 27.31318956249474 23.785820093238012 98-99 22.631627447036408 26.0919056192289 27.23687639400692 24.039590539727772 100-101 22.99174902256646 25.459573063528524 27.573472451768254 23.97520546213676 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1883.0 1 1012.0 2 158.0 3 186.0 4 194.0 5 196.0 6 300.0 7 606.0 8 1013.0 9 1303.5 10 1430.0 11 1549.5 12 1782.5 13 2075.0 14 2314.0 15 2543.0 16 2746.5 17 2899.5 18 3028.5 19 3111.0 20 3289.0 21 3672.0 22 4268.0 23 5183.5 24 6419.0 25 8196.5 26 10633.0 27 13737.5 28 16959.5 29 20681.5 30 24919.0 31 29549.5 32 34295.0 33 38664.5 34 43588.5 35 48453.5 36 54147.0 37 60460.5 38 65180.0 39 68749.5 40 72658.5 41 77838.0 42 82327.5 43 85675.0 44 90068.0 45 94086.5 46 96289.0 47 97875.5 48 99858.0 49 100555.0 50 99333.0 51 97932.5 52 96133.5 53 93993.5 54 91588.0 55 86320.0 56 77905.0 57 69509.5 58 63015.0 59 55775.0 60 47186.5 61 38931.0 62 31189.0 63 24940.5 64 19034.0 65 13574.5 66 10026.5 67 7903.5 68 6119.5 69 4563.0 70 3572.5 71 2816.5 72 2180.0 73 1715.5 74 1234.0 75 857.5 76 585.5 77 349.5 78 217.0 79 133.0 80 87.5 81 65.5 82 51.0 83 36.0 84 28.0 85 25.5 86 18.5 87 15.0 88 13.0 89 11.0 90 6.0 91 4.5 92 4.5 93 3.0 94 4.0 95 3.5 96 1.0 97 1.5 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3867646160244571 2 0.11227251314215206 3 0.11365226663933439 4 0.1257546758860481 5 0.12843533982343097 6 0.07170776032499108 7 0.08105066257734012 8 0.07833057711146635 9 0.07793636182655711 10-11 0.07584702081653812 12-13 0.08010454589355794 14-15 0.07375767980651914 16-17 0.07521627636068334 18-19 0.08574182446776009 20-21 0.08351450810802287 22-23 0.09409918850783602 24-25 0.10708858214559552 26-27 0.10829093876456872 28-29 0.10259452789763018 30-31 0.0925420381324445 32-33 0.09094546622856209 34-35 0.33825642521637495 36-37 0.5293719953403753 38-39 0.509010775874813 40-41 0.48147483822390247 42-43 0.5093852803954768 44-45 0.5197334316243444 46-47 0.5166585524020523 48-49 0.5234784768309821 50-51 0.5223746740332362 52-53 0.5213694250567177 54-55 0.5152787989048699 56-57 0.48484537890987645 58-59 0.47579813812120936 60-61 0.42656064903604507 62-63 0.50098849482691 64-65 0.5283273248353658 66-67 0.5391879559346155 68-69 0.5530446231991752 70-71 0.5491221811143284 72-73 0.535245803085523 74-75 0.5389908482921608 76-77 0.513918756171933 78-79 0.4867179015131954 80-81 0.4926902630795704 82-83 0.5080843699552763 84-85 0.5023091160313559 86-87 0.5038268448782565 88-89 0.5141158638143877 90-91 0.5332550158967313 92-93 0.5401143618541522 94-95 0.5340828679950407 96-97 0.5350486954430684 98-99 0.5364284489402508 100-101 0.5118491259261595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2536685.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.95129157854988 #Duplication Level Percentage of deduplicated Percentage of total 1 74.78967557341784 44.089479717937046 2 14.239286259527914 16.78848632311738 3 4.870545301577355 8.613748086594684 4 2.1929959660529508 5.171197785014847 5 1.1544422528665417 3.4027930929666756 6 0.6846169889290886 2.4215433440392538 7 0.46910228759230194 1.9357930015212985 8 0.3016525658047186 1.4226246689737336 9 0.21882724138858972 1.1610133661185624 >10 1.005774921290235 10.194919183011628 >50 0.050978785370686924 2.058210438988685 >100 0.021365358468284034 2.076377017907486 >500 4.6868036307345863E-4 0.2013387489849869 >1k 2.6781735032273545E-4 0.46247522482372233 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4524 0.17834299489294098 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3778 0.14893453463871154 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.942152849092418E-5 0.0 8 0.0 0.0 0.0 3.942152849092418E-5 0.0 9 0.0 0.0 0.0 7.884305698184836E-5 0.0 10-11 0.0 0.0 0.0 7.884305698184836E-5 0.0 12-13 0.0 0.0 0.0 2.3652917094554507E-4 1.971076424546209E-5 14-15 0.0 0.0 0.0 2.7595069943646926E-4 7.884305698184836E-5 16-17 0.0 0.0 0.0 2.9566146368193135E-4 7.884305698184836E-5 18-19 0.0 0.0 0.0 3.5479375641831763E-4 7.884305698184836E-5 20-21 0.0 0.0 0.0 4.5334757764562805E-4 7.884305698184836E-5 22-23 0.0 0.0 0.0 7.095875128366352E-4 7.884305698184836E-5 24-25 0.0 0.0 0.0 8.081413340639456E-4 7.884305698184836E-5 26-27 0.0 0.0 0.0 0.0010446705050094908 7.884305698184836E-5 28-29 0.0 0.0 0.0 0.0028186392871010785 7.884305698184836E-5 30-31 0.0 0.0 1.971076424546209E-5 0.0059723615663750135 7.884305698184836E-5 32-33 0.0 0.0 3.942152849092418E-5 0.013620138093614304 7.884305698184836E-5 34-35 0.0 0.0 3.942152849092418E-5 0.026392713324673737 7.884305698184836E-5 36-37 0.0 0.0 3.942152849092418E-5 0.04933604290639161 7.884305698184836E-5 38-39 0.0 0.0 3.942152849092418E-5 0.08264723448122255 7.884305698184836E-5 40-41 0.0 0.0 3.942152849092418E-5 0.11678627815436288 7.884305698184836E-5 42-43 0.0 0.0 3.942152849092418E-5 0.15506458231905024 7.884305698184836E-5 44-45 3.942152849092418E-5 0.0 3.942152849092418E-5 0.20335595472043239 7.884305698184836E-5 46-47 3.942152849092418E-5 0.0 3.942152849092418E-5 0.26097051860991805 9.855382122731044E-5 48-49 3.942152849092418E-5 0.0 3.942152849092418E-5 0.3244983117730424 1.1826458547277254E-4 50-51 3.942152849092418E-5 0.0 3.942152849092418E-5 0.3954570630567059 1.1826458547277254E-4 52-53 3.942152849092418E-5 0.0 3.942152849092418E-5 0.46556825147781455 1.1826458547277254E-4 54-55 3.942152849092418E-5 0.0 3.942152849092418E-5 0.5458501942495817 1.1826458547277254E-4 56-57 3.942152849092418E-5 0.0 3.942152849092418E-5 0.645468396746147 1.1826458547277254E-4 58-59 3.942152849092418E-5 0.0 3.942152849092418E-5 0.7589629772715177 1.1826458547277254E-4 60-61 3.942152849092418E-5 0.0 3.942152849092418E-5 0.8823720722123558 1.1826458547277254E-4 62-63 3.942152849092418E-5 0.0 3.942152849092418E-5 1.0110833627352234 1.1826458547277254E-4 64-65 3.942152849092418E-5 0.0 3.942152849092418E-5 1.1487630509897762 1.1826458547277254E-4 66-67 3.942152849092418E-5 0.0 3.942152849092418E-5 1.312855163333248 1.1826458547277254E-4 68-69 3.942152849092418E-5 0.0 3.942152849092418E-5 1.5041481303354574 1.3797534971823463E-4 70-71 3.942152849092418E-5 0.0 3.942152849092418E-5 1.715664341453511 1.5768611396369672E-4 72-73 3.942152849092418E-5 0.0 3.942152849092418E-5 1.929230472053093 1.773968782091588E-4 74-75 3.942152849092418E-5 0.0 3.942152849092418E-5 2.1567518237384617 2.1681840670008298E-4 76-77 3.942152849092418E-5 0.0 3.942152849092418E-5 2.4196145757159444 2.3652917094554507E-4 78-79 3.942152849092418E-5 0.0 3.942152849092418E-5 2.7142510796571115 2.7595069943646926E-4 80-81 3.942152849092418E-5 0.0 3.942152849092418E-5 3.0299978121051687 3.1537222792739345E-4 82-83 3.942152849092418E-5 0.0 3.942152849092418E-5 3.3537471148368834 3.1537222792739345E-4 84-85 3.942152849092418E-5 0.0 3.942152849092418E-5 3.6963596189515053 3.1537222792739345E-4 86-87 3.942152849092418E-5 0.0 3.942152849092418E-5 4.063728842958428 3.1537222792739345E-4 88-89 3.942152849092418E-5 0.0 3.942152849092418E-5 4.467562980819455 3.1537222792739345E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3715 0.0 45.37556 1 GTATCAA 6390 0.0 40.49936 1 ATCAACG 7820 0.0 32.46346 3 TCAACGC 7950 0.0 31.992186 4 CAACGCA 8070 0.0 31.515842 5 TATCAAC 8190 0.0 31.228176 2 AACGCAG 8375 0.0 30.46858 6 ACGCAGA 9745 0.0 25.94225 7 CGCAGAG 9965 0.0 25.322008 8 GTACCGT 200 2.1304004E-8 23.67109 6 GTACATG 7810 0.0 21.887894 1 GCAGAGT 11305 0.0 21.650513 9 GTGGTAT 1975 0.0 21.15768 1 TACATGG 7850 0.0 20.93614 2 ACATGGG 7970 0.0 19.670094 3 AGAGTAC 9715 0.0 19.614233 10-11 GCGTACA 220 1.383898E-6 19.375664 2 GAGTACT 5755 0.0 19.167585 12-13 CAGAGTA 10420 0.0 19.036825 10-11 TGGTATC 2065 0.0 18.807478 2 >>END_MODULE