FastQCFastQC Report
Fri 27 May 2016
SRR939126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2536685
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46600.18370432276770668No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39530.15583330212462326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48000.047.5511931
GTATCAA72500.034.111161
ATCAACG89100.027.3215543
TCAACGC90650.026.9590854
TATCAAC91400.026.8417032
CAACGCA91800.026.5696725
AACGCAG94350.025.9018656
ACGCAGA107850.022.571637
CGCAGAG109300.022.098538
GAGTACT63750.020.54485912-13
GTACATG81850.020.259451
TACATGG82100.019.7673632
GTACTTT65850.019.3491914-15
GCAGAGT125950.018.9134719
GTGGTAT23850.018.7804761
ACATGGG81500.018.7483923
CAGAGTA118750.018.72173110-11
AGAGTAC109500.017.87639210-11
TGGTATC24300.017.575272
AGTACTT66250.017.51327112-13
Produced by FastQC (version 0.11.5)