##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939126_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2536685 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.500629364702355 34.0 31.0 34.0 31.0 34.0 2 32.72606334645413 34.0 31.0 34.0 31.0 34.0 3 32.83494284863907 34.0 31.0 34.0 31.0 34.0 4 36.284393213978085 37.0 37.0 37.0 35.0 37.0 5 36.23942665328963 37.0 37.0 37.0 35.0 37.0 6 36.26856468185841 37.0 37.0 37.0 35.0 37.0 7 36.22281718069055 37.0 37.0 37.0 35.0 37.0 8 36.19591356435663 37.0 37.0 37.0 35.0 37.0 9 37.944684499652105 39.0 38.0 39.0 35.0 39.0 10-11 37.86263548686573 39.0 38.0 39.0 35.0 39.0 12-13 37.78107608946321 39.0 38.0 39.0 35.0 39.0 14-15 39.21508721027641 40.5 39.0 41.0 36.0 41.0 16-17 39.30522177566391 41.0 39.0 41.0 36.0 41.0 18-19 39.31985504703974 41.0 39.0 41.0 36.0 41.0 20-21 39.28075184739138 41.0 39.0 41.0 36.0 41.0 22-23 39.21137449072313 41.0 39.0 41.0 36.0 41.0 24-25 39.14773651438787 41.0 39.0 41.0 36.0 41.0 26-27 39.00809639352146 40.0 39.0 41.0 35.0 41.0 28-29 38.85825752901917 40.0 38.0 41.0 35.0 41.0 30-31 38.69359183343616 40.0 38.0 41.0 35.0 41.0 32-33 38.63509915499954 40.0 38.0 41.0 35.0 41.0 34-35 38.55254140738799 40.0 38.0 41.0 34.0 41.0 36-37 38.420261482998484 40.0 38.0 41.0 34.0 41.0 38-39 38.30394412392552 40.0 38.0 41.0 34.0 41.0 40-41 38.13024202847417 40.0 38.0 41.0 33.5 41.0 42-43 37.95924365855437 40.0 38.0 41.0 33.0 41.0 44-45 37.73048880724252 40.0 37.0 41.0 33.0 41.0 46-47 37.47098378395425 40.0 37.0 41.0 32.5 41.0 48-49 37.175810555902686 40.0 37.0 41.0 31.5 41.0 50-51 36.93368648452606 40.0 36.0 41.0 31.0 41.0 52-53 36.66384789597447 39.0 36.0 41.0 31.0 41.0 54-55 36.41325056126401 39.0 35.0 40.0 30.0 41.0 56-57 36.15415000285806 39.0 35.0 40.0 30.0 41.0 58-59 35.81299333579061 38.0 35.0 40.0 29.0 41.0 60-61 35.4234075180797 38.0 34.5 40.0 28.0 41.0 62-63 35.00862010852747 37.5 34.0 40.0 28.0 41.0 64-65 34.54642673410376 37.0 34.0 39.5 26.5 41.0 66-67 34.11154124378865 36.0 33.5 39.0 26.0 40.5 68-69 33.6108892117074 36.0 33.0 39.0 26.0 40.0 70-71 33.092590329504844 35.0 32.5 38.0 25.0 40.0 72-73 32.50023850024737 35.0 32.0 37.5 23.5 39.5 74-75 31.941575126592383 35.0 31.0 37.0 22.0 39.0 76-77 29.945526346393027 32.5 28.5 35.0 20.0 37.0 78-79 30.935765378831036 34.0 30.5 36.0 20.0 38.0 80-81 30.947436319448414 34.0 31.0 35.5 20.0 37.0 82-83 30.55141809093364 34.0 30.5 35.0 18.5 37.0 84-85 30.06602396434717 34.0 30.0 35.0 15.0 36.0 86-87 29.334772350528347 34.0 29.0 35.0 7.0 36.0 88-89 28.643698370116905 33.5 29.0 35.0 2.0 35.0 90-91 28.064632581499083 33.0 27.5 35.0 2.0 35.0 92-93 27.587743255469245 33.0 27.0 35.0 2.0 35.0 94-95 27.015502318971414 32.5 25.5 34.0 2.0 35.0 96-97 26.18207503099518 32.0 25.0 34.0 2.0 35.0 98-99 24.917697112570146 32.0 12.5 34.0 2.0 35.0 100-101 19.002048145512745 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1683774437606047 1101 2 0.18149179511915037 1101 3 0.21547695414925983 1101 4 0.1712485432197255 1101 5 0.1919395313920944 1101 6 0.19035667219060315 1101 7 0.15993073112089462 1101 8 0.17492577258898478 1101 9 0.28567169491903854 1101 10-11 0.2586761958402519 1101 12-13 0.23481702727472253 1101 14-15 0.33742446617767285 1101 16-17 0.30532452492678175 1101 18-19 0.3590358597561618 1101 20-21 0.3020684545963306 1101 22-23 0.2876225438381894 1101 24-25 0.32841396582609406 1101 26-27 0.3821693556741934 1101 28-29 0.42269553530214665 1101 30-31 0.46567060814567895 1101 32-33 0.5128484306266685 1101 34-35 0.5330452610465883 1101 36-37 0.5690111696912865 1101 38-39 0.48655454382591046 1101 40-41 0.5208611972213433 1101 42-43 0.5633365606770724 1101 44-45 0.5216208923821242 1101 46-47 0.5576611226915773 1101 48-49 0.49865428611490614 1101 50-51 0.41670831566754885 1101 52-53 0.49804942935595164 1101 54-55 0.3746954139977703 1101 56-57 0.47522099081643177 1101 58-59 0.4154748179869756 1101 60-61 0.3916709596445358 1101 62-63 0.34776506048051914 1101 64-65 0.3934595022488594 1101 66-67 0.40227267043939463 1101 68-69 0.49022970702080926 1101 70-71 0.49131550151829373 1101 72-73 0.49836161852151406 1101 74-75 0.40916966088469664 1101 76-77 0.43350696379747333 1101 78-79 0.5789543144027967 1101 80-81 0.6163132449368582 1101 82-83 0.558575167769412 1101 84-85 0.6391140418757608 1101 86-87 0.6329631008344236 1101 88-89 0.5787461062283157 1101 90-91 0.7751818192092124 1101 92-93 0.7528985605541756 1101 94-95 0.6305634287232706 1101 96-97 0.5588887834524563 1101 98-99 0.40181436193674 1101 100-101 -0.5941409442752068 1102 1 0.0496664699788667 1102 2 0.04236387565079269 1102 3 0.09827770422177906 1102 4 0.08584481910796882 1102 5 0.05124868215984435 1102 6 0.11099022932511105 1102 7 0.14272241043228462 1102 8 0.1620434107467119 1102 9 0.13597069563048336 1102 10-11 0.1710440741432393 1102 12-13 0.18350818332442032 1102 14-15 0.18283340702209472 1102 16-17 0.20091348066898718 1102 18-19 0.19032315124361077 1102 20-21 0.15428192931648965 1102 22-23 0.15510886036511806 1102 24-25 0.20684308974105647 1102 26-27 0.24600563136179687 1102 28-29 0.2676911713046195 1102 30-31 0.2508148959487997 1102 32-33 0.26320593147732296 1102 34-35 0.31323281679832604 1102 36-37 0.3829411208429363 1102 38-39 0.32351754013657086 1102 40-41 0.3523007479135103 1102 42-43 0.3061506995274357 1102 44-45 0.30482552122915507 1102 46-47 0.363396978783328 1102 48-49 0.35174515842115994 1102 50-51 0.26645951764790254 1102 52-53 0.3378271831679669 1102 54-55 0.2859414648726144 1102 56-57 0.4083998913064235 1102 58-59 0.27919541011956994 1102 60-61 0.16515785636577363 1102 62-63 -0.012721721706682843 1102 64-65 0.08356635330696349 1102 66-67 0.14508680928975792 1102 68-69 0.09293057839909835 1102 70-71 -0.0305193253555629 1102 72-73 0.022992497064443285 1102 74-75 0.044641582907917865 1102 76-77 0.11482228268279826 1102 78-79 0.1811570254317587 1102 80-81 0.31356028983336515 1102 82-83 0.3447118052006388 1102 84-85 0.21331819753838488 1102 86-87 0.3219889846902255 1102 88-89 0.18380934657172077 1102 90-91 0.27169427875654506 1102 92-93 0.07852153792074645 1102 94-95 -0.21903279700408262 1102 96-97 -0.2945894772353661 1102 98-99 -0.12830370008051162 1102 100-101 -0.4890199144052296 1103 1 0.04270573792484811 1103 2 0.06741980541754344 1103 3 0.07571923035733619 1103 4 0.06442008401718624 1103 5 0.0124455387858049 1103 6 0.010736425293323748 1103 7 0.0698412803683155 1103 8 0.03256539498138267 1103 9 0.11540994419286932 1103 10-11 0.022103711023774508 1103 12-13 0.0863155749377782 1103 14-15 -0.06911393823214951 1103 16-17 0.15542602347444046 1103 18-19 0.14166970580844662 1103 20-21 0.19090815290633856 1103 22-23 0.18453962860185413 1103 24-25 0.18222016136346042 1103 26-27 0.24037217122740628 1103 28-29 0.19399290129105395 1103 30-31 0.145747598507441 1103 32-33 0.16500052991370495 1103 34-35 0.12179047493621198 1103 36-37 0.13942846900473427 1103 38-39 0.23297280036142354 1103 40-41 0.3166844907254287 1103 42-43 0.17432646033379484 1103 44-45 0.12399620595487448 1103 46-47 0.210564146193029 1103 48-49 0.18191775323926862 1103 50-51 0.11191436597121651 1103 52-53 0.1277026839110036 1103 54-55 0.064761346794171 1103 56-57 0.015201516298347428 1103 58-59 -0.0497165439089855 1103 60-61 0.01072628047896984 1103 62-63 -0.049150373900261 1103 64-65 -0.04171056970783127 1103 66-67 -0.1284785013324523 1103 68-69 -0.17012892118092537 1103 70-71 -0.16834955327441747 1103 72-73 -0.22557099634513378 1103 74-75 -0.3826506289225371 1103 76-77 -0.42409181161842113 1103 78-79 -0.4165364269491967 1103 80-81 -0.29993766277372913 1103 82-83 -0.34516955100534474 1103 84-85 -0.370394226542512 1103 86-87 -0.3203979039186784 1103 88-89 -0.24462579105185256 1103 90-91 0.013013958765586864 1103 92-93 -0.055940890198399984 1103 94-95 0.03604641327568814 1103 96-97 -0.2122365301202933 1103 98-99 -0.6621903746621491 1103 100-101 -1.0605247078083444 1104 1 0.01626111957286014 1104 2 -0.020877204953670514 1104 3 0.07146560991845519 1104 4 0.038836110387897804 1104 5 0.07702887211914344 1104 6 0.024603378116950125 1104 7 0.09375080101588651 1104 8 0.046834113757071805 1104 9 0.10982711677239365 1104 10-11 0.10544336826781375 1104 12-13 0.11599052592747938 1104 14-15 -0.09891131618066851 1104 16-17 -0.06556560524875721 1104 18-19 0.14223628868329996 1104 20-21 0.12007472076189885 1104 22-23 -0.025129630868207187 1104 24-25 -0.02864342793015595 1104 26-27 -0.06273197962948274 1104 28-29 -0.034693741028554825 1104 30-31 -0.009886907120836952 1104 32-33 0.007049793178104835 1104 34-35 0.02302383514616224 1104 36-37 -0.06548708702511874 1104 38-39 -0.0303905122610999 1104 40-41 0.021715655747875928 1104 42-43 0.10766888428877053 1104 44-45 0.13163677463254686 1104 46-47 0.12223769011259122 1104 48-49 0.19105698994324882 1104 50-51 0.11887827620331137 1104 52-53 0.09135580950387379 1104 54-55 -0.018981163324077954 1104 56-57 0.08625262930662814 1104 58-59 -0.020076670861499224 1104 60-61 -0.010728377081257179 1104 62-63 -0.022083640832534 1104 64-65 -0.08013554045973592 1104 66-67 0.09290866824321142 1104 68-69 0.07699192084778161 1104 70-71 0.0434681560470338 1104 72-73 -0.21595282221625212 1104 74-75 -0.23994358013356631 1104 76-77 -0.27085406814785884 1104 78-79 0.021573138756238563 1104 80-81 0.013888481063620617 1104 82-83 -0.04539128312448781 1104 84-85 -0.24628797298531424 1104 86-87 -0.14444319102422298 1104 88-89 -0.12032043311850771 1104 90-91 -0.05873584756464467 1104 92-93 -0.2889343133312643 1104 94-95 -0.23404376173854402 1104 96-97 -0.6203502063676822 1104 98-99 -1.004156537787427 1104 100-101 -1.1725493275832157 1105 1 -0.028528196825519103 1105 2 0.023044187523325377 1105 3 0.0031722703968810606 1105 4 5.159194101693743E-4 1105 5 0.01921456992767645 1105 6 -0.032301451622153365 1105 7 0.005527760842859664 1105 8 0.028212330226068616 1105 9 0.03794145679790972 1105 10-11 0.06997815491897796 1105 12-13 -0.010477554078526907 1105 14-15 0.051754666562707996 1105 16-17 0.07305609020701098 1105 18-19 0.08676517046489352 1105 20-21 0.09508280070662778 1105 22-23 0.06565678134530373 1105 24-25 0.08515129705303082 1105 26-27 0.13926845768316554 1105 28-29 0.1649812230903578 1105 30-31 0.14885174457763384 1105 32-33 0.01538362432794571 1105 34-35 0.07395773890061719 1105 36-37 0.09067584295431175 1105 38-39 0.09718205023784066 1105 40-41 0.14589157797209396 1105 42-43 0.07481768623592444 1105 44-45 0.18678584386886854 1105 46-47 0.09738755202595684 1105 48-49 0.06177749125212273 1105 50-51 -0.04171962438962851 1105 52-53 -0.016345450048461885 1105 54-55 -0.08037505335411765 1105 56-57 -0.10159593797748556 1105 58-59 -0.23522769689959944 1105 60-61 -0.2887423304948342 1105 62-63 -0.16521871300059843 1105 64-65 -0.28000379140827647 1105 66-67 -0.30359049465920407 1105 68-69 -0.2393624234053462 1105 70-71 -0.3388165268285448 1105 72-73 -0.5989575200722683 1105 74-75 -0.408408321705533 1105 76-77 -0.6626569067741315 1105 78-79 -0.5447656965982297 1105 80-81 -0.3200570708310728 1105 82-83 -0.4012650774512174 1105 84-85 -0.5730751150743956 1105 86-87 -0.5214158846403762 1105 88-89 -0.5482358380118129 1105 90-91 -0.3586501848330279 1105 92-93 -0.0461843405197051 1105 94-95 -0.011306682374335963 1105 96-97 -0.24317063170209963 1105 98-99 -0.3034695701638803 1105 100-101 -0.898648008599249 1106 1 0.047925809414003595 1106 2 0.018635651170598067 1106 3 0.022131141177645475 1106 4 0.07875615877787823 1106 5 0.03546822750506351 1106 6 0.0661893666479827 1106 7 0.07654566059369472 1106 8 0.050120374988651406 1106 9 0.038646191618049386 1106 10-11 0.01614830086988661 1106 12-13 0.10461195201278173 1106 14-15 0.03687801166486082 1106 16-17 0.059471143430819495 1106 18-19 0.02790312093021896 1106 20-21 0.11920373481594027 1106 22-23 0.09333037136205746 1106 24-25 0.11013141333244647 1106 26-27 0.1331325689615639 1106 28-29 0.19676802851751773 1106 30-31 0.08766436362315488 1106 32-33 0.0972766719226783 1106 34-35 0.03631587479080878 1106 36-37 -0.10816539837683337 1106 38-39 0.08881291153311821 1106 40-41 0.029800365255901795 1106 42-43 0.0022609992893265485 1106 44-45 0.07646898100637145 1106 46-47 -0.006412256327266164 1106 48-49 -0.13478299194429866 1106 50-51 0.05785012418036217 1106 52-53 -0.011625817845931863 1106 54-55 -0.10410546678876642 1106 56-57 -0.15312742469280494 1106 58-59 -0.13518451767610884 1106 60-61 -0.17472295010384897 1106 62-63 -0.22036008552026942 1106 64-65 -0.2455214945230466 1106 66-67 -0.27606328110950784 1106 68-69 -0.3959441550352878 1106 70-71 -0.41174153952184156 1106 72-73 -0.45031900464514507 1106 74-75 -0.5723011726642078 1106 76-77 -0.5571693667498892 1106 78-79 -0.3843646030669703 1106 80-81 -0.31251244618582064 1106 82-83 -0.4566267483881141 1106 84-85 -0.4222778219017762 1106 86-87 -0.48427808385324767 1106 88-89 -0.6144288172241517 1106 90-91 -0.3519311748221945 1106 92-93 -0.3173397754522469 1106 94-95 -0.31122105764398 1106 96-97 -0.39470824495126067 1106 98-99 -0.34343639883517696 1106 100-101 -0.9779950594986389 1107 1 0.013418255605188278 1107 2 -0.003845222870999976 1107 3 0.028884011121128594 1107 4 -0.0027401587928039817 1107 5 -0.017443075075576076 1107 6 0.03097010044655235 1107 7 0.052104953164167966 1107 8 0.06467583816855438 1107 9 0.0726677235422315 1107 10-11 0.08216382417794676 1107 12-13 0.04019935617304782 1107 14-15 0.010474846539189286 1107 16-17 0.0065855590708565614 1107 18-19 0.053679076388355895 1107 20-21 0.010783064504636286 1107 22-23 -0.01527767455703355 1107 24-25 0.024733274281892648 1107 26-27 -7.486149583542101E-4 1107 28-29 -0.051066917190794925 1107 30-31 -0.0015495195972263787 1107 32-33 -0.006870050001424488 1107 34-35 0.01542113264483902 1107 36-37 0.09696103029657621 1107 38-39 0.0479797251610492 1107 40-41 0.06342615149865338 1107 42-43 -0.0584145816275452 1107 44-45 -0.009648743361488243 1107 46-47 0.025801652086478555 1107 48-49 -0.025410747467937256 1107 50-51 -0.12906203077560008 1107 52-53 -0.0725816748820165 1107 54-55 -0.22815546720865143 1107 56-57 -0.25667790378651745 1107 58-59 -0.31375399676142024 1107 60-61 -0.2610028567204594 1107 62-63 -0.3327518590487699 1107 64-65 -0.31588917060363286 1107 66-67 -0.3483976137794116 1107 68-69 -0.23762803716395098 1107 70-71 -0.17358661715937274 1107 72-73 -0.3434524496690372 1107 74-75 -0.41589187715450393 1107 76-77 -0.47563368455571364 1107 78-79 -0.354734063677153 1107 80-81 -0.22095295626736444 1107 82-83 -0.3868332709487703 1107 84-85 -0.34088647666037986 1107 86-87 -0.42237957530010206 1107 88-89 -0.49594444970342977 1107 90-91 -0.20543179205949258 1107 92-93 -0.12431665634644062 1107 94-95 -0.21519342052912194 1107 96-97 -0.6027046171075625 1107 98-99 -0.7096171485895404 1107 100-101 -1.3006716019620477 1108 1 -0.15244846307317772 1108 2 -0.06892151116595358 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-0.5484451564662862 2316 96-97 -0.33686409561290986 2316 98-99 -0.018483977084720493 2316 100-101 1.0506624964891955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 18.0 5 33.0 6 64.0 7 190.0 8 461.0 9 956.0 10 1656.0 11 2605.0 12 3810.0 13 5437.0 14 7005.0 15 9019.0 16 11784.0 17 16356.0 18 16900.0 19 13309.0 20 13438.0 21 14433.0 22 15948.0 23 17423.0 24 19885.0 25 22994.0 26 27428.0 27 32699.0 28 39825.0 29 48286.0 30 59722.0 31 73070.0 32 91921.0 33 120166.0 34 167925.0 35 249233.0 36 403743.0 37 616300.0 38 395264.0 39 17376.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.52258340360337 19.505576075725365 15.666813145049229 23.30502737562204 2 15.54461827148424 20.199906570977475 39.98801585533876 24.267459302199523 3 19.118692309056897 23.87486029995841 29.734633980963345 27.271813410021345 4 13.616866106749557 17.29694463443431 36.468580056254524 32.61760920256161 5 14.581274379751527 36.569893384476195 33.99598294624677 14.852849289525501 6 32.39649384925602 35.64979490949803 17.518138830796886 14.43557241044907 7 28.910684613974535 31.896747132576568 20.743253498167885 18.44931475528101 8 25.849563505125783 35.56681259202463 19.759686362319325 18.82393754053026 9 25.770917555786387 17.553460520324755 20.387119409780876 36.288502514107975 10-11 25.10859645561037 26.6048405694834 27.028720554582065 21.257842420324163 12-13 25.665307280959205 23.667049712518505 27.67491824960529 22.992724756917 14-15 22.835807215569247 25.341555406847764 25.59976819981334 26.222869177769653 16-17 22.567144256075387 28.30846698845869 26.37568457022472 22.748704185241195 18-19 22.525816173470496 27.193896758959035 28.23555151703897 22.0447355505315 20-21 23.302577970855666 26.40712189333717 28.20123507648762 22.089065059319545 22-23 23.003033486617376 26.192629356818053 28.0510390529372 22.753298103627372 24-25 22.839215621344884 26.435013756153214 28.136428556504693 22.589342065997208 26-27 22.794296064475294 26.61210411813138 27.997107939191817 22.596491878201505 28-29 22.690129576618613 26.621186040282108 28.147666767388692 22.541017615710587 30-31 22.894348332567898 26.47427646712146 27.920967719681393 22.710407480629247 32-33 22.783006716050025 26.451358062068813 27.953969048969235 22.811666172911927 34-35 23.062098864094693 26.579813601294124 27.792422382826622 22.565665151784565 36-37 23.11436818321622 26.428182743038303 27.840218137080935 22.617230936664537 38-39 22.867744673871343 26.581199681573743 27.894116566248318 22.6569390783066 40-41 23.061072662859253 26.41611083820168 27.8076992525786 22.71511724636047 42-43 23.004852443608282 26.46050352184585 27.62498354001295 22.90966049453292 44-45 23.01714424933592 26.57865231881807 27.585376295930686 22.818827135915328 46-47 23.088189145247792 26.71329302658574 27.34872354081216 22.84979428735431 48-49 23.19886194634062 26.611997935040506 27.361245504090792 22.827894614528084 50-51 23.14218122886255 26.845322077636396 27.203744592814754 22.808752100686295 52-53 23.147276859365668 26.870699357626194 27.018687775581125 22.963336007427017 54-55 23.039123895950816 26.75629808194553 27.03451551927023 23.17006250283342 56-57 22.932438569407935 26.892352378144064 27.143526745940765 23.031682306507236 58-59 23.064045669808483 26.729520078308223 27.233335554116206 22.97309869776709 60-61 23.11999667274364 26.68386668976823 27.325940437774687 22.870196199713437 62-63 22.894085237515736 26.71135941731092 27.37234852382253 23.02220682135081 64-65 22.900753879287063 26.57522057956811 27.281418174550083 23.242607366594747 66-67 22.891269647599792 26.779849509172198 27.085924530667228 23.242956312560782 68-69 22.800327686471224 26.724424429672972 27.225060918854627 23.250186965001177 70-71 22.84860245616075 26.579912937468524 27.196032613462663 23.37545199290806 72-73 22.893855563461763 26.659025460394176 27.060316909667538 23.386802066476523 74-75 22.838586580517486 26.77906401464904 26.88205275783158 23.500296647001896 76-77 22.854154709873363 26.65609437877977 27.054350543158197 23.43540036818867 78-79 22.783157153494198 26.62965447644514 27.072251246559187 23.514937123501472 80-81 22.773913031480365 26.75816171277654 26.994376303009076 23.47354895273402 82-83 22.782824828467074 26.70262567090514 27.03755097696403 23.47699852366376 84-85 22.81132659356601 26.510268322633674 27.02353662358551 23.65486846021481 86-87 22.764754782013657 26.50111947491723 27.12035771105807 23.61376803201104 88-89 22.722135304913486 26.44267997029328 27.09726495509947 23.737919769693764 90-91 22.62981173016128 26.561876787799406 27.070789087225904 23.73752239481341 92-93 22.623837804063175 26.392496506267037 27.077149902333165 23.90651578733662 94-95 22.48375783429118 26.368257463630187 27.099507250507504 24.048477451571134 96-97 22.598676066865245 26.278164722133308 27.096496843911183 24.026662367090264 98-99 22.39442011037257 26.301434827635006 26.98904592260517 24.315099139387257 100-101 22.864023618277358 25.29060135503914 27.537160291633217 24.30821473505029 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 141.0 1 107.0 2 89.0 3 112.0 4 104.5 5 103.0 6 181.0 7 433.0 8 762.5 9 959.0 10 1001.0 11 1014.0 12 1132.5 13 1325.0 14 1485.0 15 1624.0 16 1786.0 17 1965.0 18 2127.5 19 2335.5 20 2612.5 21 3129.0 22 3938.0 23 4944.0 24 6200.5 25 7973.5 26 10497.5 27 13774.0 28 16883.5 29 20540.5 30 24858.5 31 29592.5 32 34694.5 33 38907.0 34 43496.5 35 48462.0 36 54405.0 37 60678.5 38 65588.5 39 69500.5 40 73403.0 41 78579.0 42 83185.0 43 86380.0 44 90630.0 45 95050.0 46 97346.5 47 98709.5 48 100622.5 49 101547.5 50 100242.0 51 98938.0 52 97625.5 53 95207.0 54 92526.5 55 87260.0 56 78701.0 57 70231.5 58 63622.5 59 56192.0 60 47204.0 61 38732.5 62 31079.5 63 24544.5 64 18376.5 65 12979.5 66 9515.5 67 7379.0 68 5645.5 69 4145.5 70 3078.5 71 2420.5 72 1888.5 73 1464.0 74 1062.5 75 729.0 76 476.0 77 263.0 78 132.5 79 76.5 80 45.5 81 24.5 82 13.5 83 7.0 84 2.5 85 1.5 86 2.5 87 1.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4224016777802526 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.898767485911732E-4 16-17 0.0022470271239826783 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.884305698184836E-5 26-27 0.003370540685974017 28-29 1.971076424546209E-5 30-31 0.0 32-33 1.971076424546209E-5 34-35 0.011589929376331708 36-37 0.02621531644646458 38-39 0.01821274616280697 40-41 0.009106373081403486 42-43 0.009106373081403486 44-45 0.0035873590926741006 46-47 0.005400749403256613 48-49 0.002897482344082927 50-51 5.519013988729385E-4 52-53 0.0 54-55 0.0 56-57 3.942152849092418E-5 58-59 0.0016557041966188154 60-61 0.0023850024737009125 62-63 0.0012614889117095738 64-65 0.002838350051346541 66-67 0.0020499194815280573 68-69 0.0044940542479653565 70-71 9.855382122731044E-5 72-73 0.0 74-75 0.0 76-77 0.09407947774359054 78-79 0.015906586746087907 80-81 3.3508299217285554E-4 82-83 0.0 84-85 0.0 86-87 0.10582709323388596 88-89 0.420115229127779 90-91 0.42218485937355243 92-93 0.0 94-95 1.971076424546209E-5 96-97 3.942152849092418E-5 98-99 1.5768611396369672E-4 100-101 2.1681840670008298E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2536685.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.05090466631908 #Duplication Level Percentage of deduplicated Percentage of total 1 73.17583028774341 41.74747317624794 2 14.992891438106657 17.107160402157888 3 5.215427479314143 8.926345677493561 4 2.3976088677684095 5.471430197687071 5 1.2271066281709864 3.500377162959561 6 0.757104796126266 2.591610812772753 7 0.47830862658523643 1.9101557899474635 8 0.3400336998714943 1.5519384155763503 9 0.2453380646142741 1.2597082681795364 >10 1.0902605105737035 10.798966583574824 >50 0.05578868318936428 2.1879135565913663 >100 0.02347272240659098 2.238416640350962 >500 5.521303530204553E-4 0.22411919958752374 >1k 2.760651764985479E-4 0.4843841168731888 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4660 0.18370432276770668 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3953 0.15583330212462326 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.942152849092418E-5 0.0 3 0.0 0.0 0.0 3.942152849092418E-5 0.0 4 0.0 0.0 0.0 7.884305698184836E-5 0.0 5 0.0 0.0 0.0 7.884305698184836E-5 0.0 6 0.0 0.0 0.0 1.1826458547277254E-4 0.0 7 0.0 0.0 0.0 1.1826458547277254E-4 0.0 8 0.0 0.0 0.0 1.1826458547277254E-4 0.0 9 0.0 0.0 0.0 1.5768611396369672E-4 0.0 10-11 0.0 0.0 0.0 1.5768611396369672E-4 0.0 12-13 0.0 0.0 0.0 3.3508299217285554E-4 0.0 14-15 0.0 0.0 0.0 3.9421528490924177E-4 0.0 16-17 1.971076424546209E-5 0.0 0.0 4.7305834189109014E-4 0.0 18-19 3.942152849092418E-5 0.0 0.0 5.519013988729385E-4 3.942152849092418E-5 20-21 3.942152849092418E-5 0.0 0.0 6.110336916093248E-4 3.942152849092418E-5 22-23 3.942152849092418E-5 0.0 0.0 9.264059195367182E-4 7.884305698184836E-5 24-25 3.942152849092418E-5 0.0 0.0 0.0010249597407640287 7.884305698184836E-5 26-27 3.942152849092418E-5 0.0 0.0 0.0012417781474641117 7.884305698184836E-5 28-29 5.913229273638627E-5 0.0 0.0 0.003134011515028472 7.884305698184836E-5 30-31 7.884305698184836E-5 0.0 0.0 0.006386287615529717 7.884305698184836E-5 32-33 7.884305698184836E-5 0.0 0.0 0.014191750256732704 7.884305698184836E-5 34-35 7.884305698184836E-5 0.0 0.0 0.027003747016283063 7.884305698184836E-5 36-37 7.884305698184836E-5 1.971076424546209E-5 0.0 0.049986498126491856 7.884305698184836E-5 38-39 7.884305698184836E-5 5.913229273638627E-5 0.0 0.08365248345774111 7.884305698184836E-5 40-41 7.884305698184836E-5 7.884305698184836E-5 0.0 0.11859966846494539 7.884305698184836E-5 42-43 7.884305698184836E-5 7.884305698184836E-5 0.0 0.15756784937822393 7.884305698184836E-5 44-45 7.884305698184836E-5 7.884305698184836E-5 0.0 0.20597748636507884 7.884305698184836E-5 46-47 7.884305698184836E-5 7.884305698184836E-5 0.0 0.2637497363685282 9.855382122731044E-5 48-49 7.884305698184836E-5 7.884305698184836E-5 0.0 0.32820393545118925 1.1826458547277254E-4 50-51 7.884305698184836E-5 7.884305698184836E-5 0.0 0.39971458813372573 1.1826458547277254E-4 52-53 7.884305698184836E-5 7.884305698184836E-5 0.0 0.470022884197289 1.1826458547277254E-4 54-55 7.884305698184836E-5 7.884305698184836E-5 0.0 0.5507187530182107 1.1826458547277254E-4 56-57 7.884305698184836E-5 7.884305698184836E-5 0.0 0.6513619152555401 1.3797534971823463E-4 58-59 7.884305698184836E-5 7.884305698184836E-5 0.0 0.7657040586434658 1.5768611396369672E-4 60-61 7.884305698184836E-5 7.884305698184836E-5 0.0 0.8902169563820498 1.9710764245462088E-4 62-63 7.884305698184836E-5 7.884305698184836E-5 0.0 1.0195787021250173 2.3652917094554507E-4 64-65 7.884305698184836E-5 7.884305698184836E-5 0.0 1.1585198792912799 2.3652917094554507E-4 66-67 7.884305698184836E-5 7.884305698184836E-5 0.0 1.323735505196743 2.3652917094554507E-4 68-69 7.884305698184836E-5 7.884305698184836E-5 0.0 1.5169404163307623 2.3652917094554507E-4 70-71 7.884305698184836E-5 7.884305698184836E-5 0.0 1.7305065469303442 2.3652917094554507E-4 72-73 7.884305698184836E-5 7.884305698184836E-5 0.0 1.9457480924907902 2.3652917094554507E-4 74-75 7.884305698184836E-5 7.884305698184836E-5 0.0 2.175989529642033 2.3652917094554507E-4 76-77 7.884305698184836E-5 7.884305698184836E-5 0.0 2.4410401764507617 2.3652917094554507E-4 78-79 7.884305698184836E-5 7.884305698184836E-5 0.0 2.7379039967516663 2.3652917094554507E-4 80-81 7.884305698184836E-5 7.884305698184836E-5 0.0 3.054754531997469 2.3652917094554507E-4 82-83 7.884305698184836E-5 7.884305698184836E-5 0.0 3.379154289949284 2.3652917094554507E-4 84-85 9.855382122731044E-5 7.884305698184836E-5 0.0 3.7222004308773062 2.9566146368193135E-4 86-87 1.1826458547277254E-4 7.884305698184836E-5 0.0 4.091836392772457 3.1537222792739345E-4 88-89 1.1826458547277254E-4 7.884305698184836E-5 0.0 4.499730948068049 3.1537222792739345E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4800 0.0 47.551193 1 GTATCAA 7250 0.0 34.11116 1 ATCAACG 8910 0.0 27.321554 3 TCAACGC 9065 0.0 26.959085 4 TATCAAC 9140 0.0 26.841703 2 CAACGCA 9180 0.0 26.569672 5 AACGCAG 9435 0.0 25.901865 6 ACGCAGA 10785 0.0 22.57163 7 CGCAGAG 10930 0.0 22.09853 8 GAGTACT 6375 0.0 20.544859 12-13 GTACATG 8185 0.0 20.25945 1 TACATGG 8210 0.0 19.767363 2 GTACTTT 6585 0.0 19.34919 14-15 GCAGAGT 12595 0.0 18.913471 9 GTGGTAT 2385 0.0 18.780476 1 ACATGGG 8150 0.0 18.748392 3 CAGAGTA 11875 0.0 18.721731 10-11 AGAGTAC 10950 0.0 17.876392 10-11 TGGTATC 2430 0.0 17.57527 2 AGTACTT 6625 0.0 17.513271 12-13 >>END_MODULE