##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939125_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1390086 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0617156060848 33.0 31.0 34.0 30.0 34.0 2 32.13335505860788 34.0 31.0 34.0 30.0 34.0 3 32.18694526813449 34.0 31.0 34.0 30.0 34.0 4 35.63776629647374 37.0 35.0 37.0 33.0 37.0 5 35.70172133234922 37.0 35.0 37.0 35.0 37.0 6 35.814697076296 37.0 36.0 37.0 35.0 37.0 7 35.74773287408117 37.0 35.0 37.0 35.0 37.0 8 35.75116287769246 37.0 36.0 37.0 35.0 37.0 9 37.39435329900452 39.0 37.0 39.0 35.0 39.0 10-11 37.30517860046069 39.0 37.0 39.0 34.0 39.0 12-13 37.227422619895464 39.0 37.0 39.0 34.0 39.0 14-15 38.444569616556095 40.0 38.0 41.0 34.0 41.0 16-17 38.41540523392078 40.0 38.0 41.0 34.0 41.0 18-19 38.408777586422715 40.0 38.0 41.0 34.0 41.0 20-21 38.32252141234427 40.0 38.0 41.0 34.0 41.0 22-23 38.23695692208971 40.0 38.0 41.0 34.0 41.0 24-25 38.25268580505091 40.0 38.0 41.0 34.0 41.0 26-27 38.1378630530773 40.0 38.0 41.0 33.5 41.0 28-29 38.02459092459027 40.0 38.0 41.0 33.0 41.0 30-31 37.86066149864109 40.0 37.5 41.0 33.0 41.0 32-33 37.62324525245201 40.0 37.0 41.0 33.0 41.0 34-35 37.27727421181136 40.0 36.5 41.0 31.5 41.0 36-37 36.985285442771165 40.0 36.0 41.0 31.0 41.0 38-39 36.71736892537584 39.5 35.5 41.0 30.5 41.0 40-41 36.58681477261119 39.0 35.5 41.0 30.0 41.0 42-43 36.36477815041659 39.0 35.0 41.0 30.0 41.0 44-45 36.04404727477293 39.0 35.0 41.0 29.5 41.0 46-47 35.57284441394273 39.0 35.0 41.0 27.5 41.0 48-49 35.46444248773098 39.0 35.0 41.0 26.0 41.0 50-51 34.41711735820661 38.0 34.0 40.0 24.5 40.5 52-53 34.59049548013576 38.5 34.5 39.5 25.0 40.5 54-55 35.117381586463 39.0 35.0 40.5 26.0 41.0 56-57 34.98893989292749 39.0 35.0 41.0 25.0 41.0 58-59 34.81516215543499 39.0 35.0 41.0 25.0 41.0 60-61 34.464079200855195 38.0 34.5 40.0 23.5 41.0 62-63 34.11902393089348 38.0 34.0 40.0 22.5 41.0 64-65 33.72542957773835 37.0 34.0 40.0 22.0 41.0 66-67 33.28774334825327 36.5 33.5 39.0 20.5 41.0 68-69 32.85945437908158 36.0 33.0 39.0 19.0 41.0 70-71 32.388356547724385 35.5 33.0 38.5 17.0 40.0 72-73 31.90898692598875 35.0 32.0 37.5 12.5 40.0 74-75 31.462595479704135 35.0 32.0 37.0 8.0 39.0 76-77 31.022810099519024 35.0 31.0 36.5 6.5 39.0 78-79 30.587319417647542 35.0 31.0 36.0 2.0 38.5 80-81 30.12926034792092 35.0 31.0 36.0 2.0 37.0 82-83 29.702708681333384 34.0 30.0 35.0 2.0 37.0 84-85 29.32287570697065 34.0 30.0 35.0 2.0 36.5 86-87 28.975278148258454 34.0 29.0 35.0 2.0 36.0 88-89 28.54141146662868 34.0 29.0 35.0 2.0 36.0 90-91 28.117899180338483 34.0 29.0 35.0 2.0 35.0 92-93 27.69054540510443 34.0 27.5 35.0 2.0 35.0 94-95 27.21251814635929 34.0 26.5 35.0 2.0 35.0 96-97 26.62373083391963 34.0 25.0 35.0 2.0 35.0 98-99 25.92356120412694 33.0 24.0 35.0 2.0 35.0 100-101 23.4465176974662 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30599513586567184 1101 2 0.38629869115226256 1101 3 0.28718361917250945 1101 4 0.30557352074782074 1101 5 0.3732162395502243 1101 6 0.37249150819788923 1101 7 0.15783476437406563 1101 8 0.2595205567052403 1101 9 0.4833994971344211 1101 10-11 0.4051428489244131 1101 12-13 0.49488255427612415 1101 14-15 0.5268876758150398 1101 16-17 0.47646561579464475 1101 18-19 0.4288151841038257 1101 20-21 0.49476086853870527 1101 22-23 0.5404228369746065 1101 24-25 0.5348878405236448 1101 26-27 0.4640797253326525 1101 28-29 0.43634027817936527 1101 30-31 0.3715722848797043 1101 32-33 0.5640855658084689 1101 34-35 0.5837564750562976 1101 36-37 0.41724118312759373 1101 38-39 0.5243467928632128 1101 40-41 0.4608537476661283 1101 42-43 0.36923624421887524 1101 44-45 0.24325576183868236 1101 46-47 0.2516796997510795 1101 48-49 0.3677670579879688 1101 50-51 0.4334437292323088 1101 52-53 0.497049431249323 1101 54-55 0.34027847979447756 1101 56-57 0.3697413810609902 1101 58-59 0.48070113480409304 1101 60-61 0.32369017649506304 1101 62-63 0.36546023360637037 1101 64-65 0.36362967101251087 1101 66-67 0.2429022036119548 1101 68-69 0.13037495756842077 1101 70-71 0.22666643372030393 1101 72-73 0.19227196663000257 1101 74-75 0.3509350469754864 1101 76-77 0.3598777170448493 1101 78-79 0.3094539632831008 1101 80-81 0.29813886457518635 1101 82-83 -0.030949235484381887 1101 84-85 -0.18588317250448583 1101 86-87 -0.08894875181932704 1101 88-89 -0.09337255111304188 1101 90-91 -0.25971307406003064 1101 92-93 -0.4105287149166834 1101 94-95 -0.5215173858134747 1101 96-97 -0.7967041463887767 1101 98-99 -0.7527937834671938 1101 100-101 -0.607267481189929 1102 1 0.21745237844857002 1102 2 0.31290427579450153 1102 3 0.3431740205686751 1102 4 0.4178597336623042 1102 5 0.35068133553626524 1102 6 0.29151855880871125 1102 7 0.39470212912100777 1102 8 0.4684210802654505 1102 9 0.4801796018464586 1102 10-11 0.3117418890640309 1102 12-13 0.49899468342098174 1102 14-15 0.541460101644013 1102 16-17 0.7069150049743982 1102 18-19 0.638762828082875 1102 20-21 0.6538424566713346 1102 22-23 0.6518495385452852 1102 24-25 0.5671762349389979 1102 26-27 0.6228886258562198 1102 28-29 0.5615497022631359 1102 30-31 0.6193493354905257 1102 32-33 0.539537136489102 1102 34-35 0.4646487089131952 1102 36-37 0.30876823373841233 1102 38-39 0.492348538063915 1102 40-41 0.4051753008947472 1102 42-43 0.40561495277035675 1102 44-45 0.6036113464809176 1102 46-47 0.4332002058592792 1102 48-49 0.49649306147836825 1102 50-51 0.49567322312411477 1102 52-53 0.5609608623138911 1102 54-55 0.4902762982936082 1102 56-57 0.5196083095077597 1102 58-59 0.48692931979536525 1102 60-61 0.1786050979610323 1102 62-63 0.10011773796937717 1102 64-65 0.15795776874374923 1102 66-67 0.20033675858577027 1102 68-69 0.05891989648640106 1102 70-71 -0.1570115767508966 1102 72-73 -0.27143004035080764 1102 74-75 -0.1887355463927527 1102 76-77 -0.285155005468237 1102 78-79 -0.2947999634184697 1102 80-81 -0.13397500977036358 1102 82-83 -0.23154260372172786 1102 84-85 -0.22582209048005453 1102 86-87 -0.2321337430933248 1102 88-89 -0.20333546559820803 1102 90-91 -0.1658518175155237 1102 92-93 -0.20436815645245687 1102 94-95 -0.4019209634749039 1102 96-97 -0.4938005687273481 1102 98-99 -0.7710813052822054 1102 100-101 -0.5714232403522317 1103 1 0.3878485419181317 1103 2 0.34331711983119817 1103 3 0.45706267778302134 1103 4 0.4287688245713994 1103 5 0.48918911980093327 1103 6 0.3958770519974806 1103 7 0.4657265939528159 1103 8 0.36352811390459294 1103 9 0.43005588764017944 1103 10-11 0.4140267792502996 1103 12-13 0.3824513834487817 1103 14-15 0.37343118866097313 1103 16-17 0.5327483383077336 1103 18-19 0.537094771368956 1103 20-21 0.5696240107441781 1103 22-23 0.4987559883645858 1103 24-25 0.49451533696847605 1103 26-27 0.5326669133179891 1103 28-29 0.5691796716821003 1103 30-31 0.5368121517262736 1103 32-33 0.5630695940926529 1103 34-35 0.37356968472636964 1103 36-37 0.310309796151536 1103 38-39 0.4442014710636357 1103 40-41 0.44108960448107126 1103 42-43 0.36712315404920304 1103 44-45 0.5212361032226482 1103 46-47 0.6813927969073745 1103 48-49 0.5407271454372236 1103 50-51 0.49149821617387346 1103 52-53 0.42270919425718034 1103 54-55 0.4399736155023959 1103 56-57 0.3828933386987501 1103 58-59 0.3717308905487755 1103 60-61 0.5420061960983702 1103 62-63 0.4435765230680673 1103 64-65 0.4581801763056035 1103 66-67 0.3501032306458214 1103 68-69 0.2411533549239806 1103 70-71 0.27402192338810494 1103 72-73 0.22708573668560916 1103 74-75 0.28028898794141455 1103 76-77 0.18926360726459635 1103 78-79 0.09292244414338668 1103 80-81 0.2726864442190724 1103 82-83 0.24931922558046793 1103 84-85 0.24804919114351875 1103 86-87 0.3965860233787346 1103 88-89 0.5013569864451632 1103 90-91 0.3429353662487209 1103 92-93 0.17850090109549654 1103 94-95 -0.0033412442644511486 1103 96-97 0.47091342904857925 1103 98-99 0.10731979285513304 1103 100-101 -0.13528166403864006 1104 1 0.1345848501771627 1104 2 0.20412398500290152 1104 3 0.2874430027980779 1104 4 0.14722968519703983 1104 5 0.3210434787780514 1104 6 0.3765091350068843 1104 7 0.30906669562612876 1104 8 0.2948615756881736 1104 9 0.28206705472744886 1104 10-11 0.39267551857399496 1104 12-13 0.3604210431409598 1104 14-15 0.42613969238175997 1104 16-17 0.5206320216679927 1104 18-19 0.4866626665319984 1104 20-21 0.5437138890892186 1104 22-23 0.4678821179744759 1104 24-25 0.5462358741420132 1104 26-27 0.5988027346338143 1104 28-29 0.5630548180412731 1104 30-31 0.5370061744781438 1104 32-33 0.45457281231031743 1104 34-35 0.33116122910706025 1104 36-37 0.42852859992043335 1104 38-39 0.548630175112919 1104 40-41 0.3116151231887656 1104 42-43 0.44742701523670547 1104 44-45 0.4486685624206004 1104 46-47 0.499718111082764 1104 48-49 0.33664384230766586 1104 50-51 0.3201656840826388 1104 52-53 0.3659063388890118 1104 54-55 0.2975386838617311 1104 56-57 0.3221184064382925 1104 58-59 0.3368249848465297 1104 60-61 0.36900830205365054 1104 62-63 0.3742137261223064 1104 64-65 0.29751081128548407 1104 66-67 0.3904336891188933 1104 68-69 0.3768352168956568 1104 70-71 0.3839833074709631 1104 72-73 0.3009642784429474 1104 74-75 0.307875250591632 1104 76-77 0.16681524224312483 1104 78-79 0.2020430629681762 1104 80-81 0.18201112377836992 1104 82-83 0.028532786692917256 1104 84-85 0.2079231976190279 1104 86-87 0.17236544915223106 1104 88-89 0.22865362971681336 1104 90-91 0.11414683622707145 1104 92-93 -0.13351193674325046 1104 94-95 -0.23115292362568596 1104 96-97 -0.12530231886197285 1104 98-99 -0.1811869864884521 1104 100-101 0.2973307984199778 1105 1 0.17387980427054117 1105 2 0.21999729905031273 1105 3 0.3432000831187949 1105 4 0.1975229253222892 1105 5 0.28064992682201506 1105 6 0.3725817771995139 1105 7 0.2211322280250485 1105 8 0.2134357822167985 1105 9 0.32911437864410686 1105 10-11 0.2579644899728777 1105 12-13 0.3835693292376092 1105 14-15 0.38623422620963765 1105 16-17 0.3960201907536032 1105 18-19 0.3575278641694837 1105 20-21 0.376747094468719 1105 22-23 0.4171108323170074 1105 24-25 0.42215585268826317 1105 26-27 0.43103952133870393 1105 28-29 0.4104096327629989 1105 30-31 0.5184815862256258 1105 32-33 0.7002231161736745 1105 34-35 0.3560490697792673 1105 36-37 0.48529456359674583 1105 38-39 0.6280595778928344 1105 40-41 0.5259621894272257 1105 42-43 0.44501217276501137 1105 44-45 0.5311110123456615 1105 46-47 0.49663478217577506 1105 48-49 0.55365157524475 1105 50-51 0.46261321243177633 1105 52-53 0.4694582560588856 1105 54-55 0.4756067580637193 1105 56-57 0.4468727441509017 1105 58-59 0.44304566569218196 1105 60-61 0.5809771241572363 1105 62-63 0.6803639956544814 1105 64-65 0.6892862905293669 1105 66-67 0.4791739678881086 1105 68-69 0.5990946492263092 1105 70-71 0.330934827953719 1105 72-73 0.5061484814348134 1105 74-75 0.3706553250320006 1105 76-77 0.4053725941040689 1105 78-79 0.3629219023928094 1105 80-81 0.2633041268241314 1105 82-83 0.31335982878295 1105 84-85 0.41325703274902637 1105 86-87 0.42805470919215693 1105 88-89 0.17354204041622623 1105 90-91 0.24057627657695946 1105 92-93 0.0772517312780927 1105 94-95 -0.07246059191649934 1105 96-97 -0.09927604319941352 1105 98-99 0.2521447706333255 1105 100-101 0.3645140090463208 1106 1 0.24653309593735884 1106 2 0.31651414126535116 1106 3 0.3205693867869073 1106 4 0.21005704307934536 1106 5 0.3164698258202705 1106 6 0.36972109991484103 1106 7 0.231884490854938 1106 8 0.3599009008932512 1106 9 0.40138289033674823 1106 10-11 0.35601505797284716 1106 12-13 0.3136340705659748 1106 14-15 0.30259612556030646 1106 16-17 0.3937348853929379 1106 18-19 0.35789586246255567 1106 20-21 0.5928951472543034 1106 22-23 0.5107172515497709 1106 24-25 0.48430441132165925 1106 26-27 0.46800447040031656 1106 28-29 0.40350037490888013 1106 30-31 0.44914567330815913 1106 32-33 0.45528638910494124 1106 34-35 0.422135629690473 1106 36-37 0.4649192053378144 1106 38-39 0.5872737996483721 1106 40-41 0.5599193218215035 1106 42-43 0.44965830105137883 1106 44-45 0.6686132149413027 1106 46-47 0.7303283822419502 1106 48-49 0.4560894740344281 1106 50-51 0.3157815938266495 1106 52-53 0.13450159475036116 1106 54-55 0.26769221757611916 1106 56-57 0.32949807034483314 1106 58-59 0.4611165395263086 1106 60-61 0.397160778080611 1106 62-63 0.5094021176405263 1106 64-65 0.44390694662117625 1106 66-67 0.40948474064855844 1106 68-69 0.3334247544834099 1106 70-71 0.33912818408617085 1106 72-73 0.1759410358823743 1106 74-75 0.10891953581950631 1106 76-77 0.18214506927017737 1106 78-79 0.26250571670111 1106 80-81 0.31070548350318106 1106 82-83 0.34591629014972014 1106 84-85 0.26282514419856895 1106 86-87 0.14362858874524065 1106 88-89 0.20150571526875538 1106 90-91 0.20489182971915199 1106 92-93 0.29504888390628636 1106 94-95 0.01863396926052019 1106 96-97 0.20275585877638846 1106 98-99 0.3466298307417084 1106 100-101 0.281822275342833 1107 1 0.06687516705427043 1107 2 0.1537513522562648 1107 3 0.08302784365712768 1107 4 0.1856858206188292 1107 5 0.13891846697055144 1107 6 0.11396108754434664 1107 7 0.13516939548782858 1107 8 0.12639209475820223 1107 9 0.21569434447514624 1107 10-11 0.3085903398386378 1107 12-13 0.3779645734759569 1107 14-15 0.35445360489239164 1107 16-17 0.4694050099719007 1107 18-19 0.19206117890746555 1107 20-21 0.2606946555718892 1107 22-23 0.32158592035438716 1107 24-25 0.31915212199547227 1107 26-27 0.2949738332525129 1107 28-29 0.14672461480686394 1107 30-31 0.12453611709971568 1107 32-33 0.19293606202633384 1107 34-35 0.1485336414469245 1107 36-37 0.1939431462821446 1107 38-39 0.350367028818539 1107 40-41 0.11304074317360602 1107 42-43 0.23007022513417752 1107 44-45 0.2377199171955624 1107 46-47 0.4293689964639782 1107 48-49 0.6401112523829227 1107 50-51 0.6847899147782783 1107 52-53 0.6924020167366791 1107 54-55 0.8046922153350877 1107 56-57 0.7058508132558856 1107 58-59 0.6484516836134588 1107 60-61 0.42770492304848773 1107 62-63 0.5018050193286925 1107 64-65 0.6399417767397537 1107 66-67 0.4069484527386962 1107 68-69 0.6224724951870471 1107 70-71 0.7459656846184188 1107 72-73 0.5849898746916722 1107 74-75 0.5711626295192893 1107 76-77 0.5151142349115752 1107 78-79 0.5629149291352533 1107 80-81 0.4787167693400818 1107 82-83 0.4145243627950137 1107 84-85 0.42822338678131544 1107 86-87 0.41300118944040776 1107 88-89 0.46149399467165964 1107 90-91 0.570803604773328 1107 92-93 0.7375059065160556 1107 94-95 0.6455234393236928 1107 96-97 0.7136663228268745 1107 98-99 0.8408446067617739 1107 100-101 0.9359811824363717 1108 1 -0.27390125324621906 1108 2 -0.1066078298548021 1108 3 -0.03691218632788207 1108 4 -0.11995391270600209 1108 5 -0.2036442942020571 1108 6 -0.15419738641016778 1108 7 -0.010146244564488427 1108 8 -0.13101643501946114 1108 9 0.03926128440943444 1108 10-11 -0.0013557509457555739 1108 12-13 0.020911716925503754 1108 14-15 -0.04506472105102688 1108 16-17 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-1.4314970172261567 2316 74-75 -1.394893893826481 2316 76-77 -1.5262132400988087 2316 78-79 -1.2058335223581196 2316 80-81 -1.195113616651355 2316 82-83 -1.354249879525451 2316 84-85 -1.4609405337964674 2316 86-87 -1.3310931339562693 2316 88-89 -1.5165002992135506 2316 90-91 -1.577601676511577 2316 92-93 -1.795107017140559 2316 94-95 -1.9323940317208148 2316 96-97 -1.877367263594806 2316 98-99 -1.8727571372055003 2316 100-101 -1.4439775268722599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4295.0 3 964.0 4 982.0 5 1071.0 6 1208.0 7 1534.0 8 1973.0 9 2389.0 10 2965.0 11 3967.0 12 5741.0 13 8770.0 14 7762.0 15 8433.0 16 11205.0 17 15878.0 18 14509.0 19 8261.0 20 8237.0 21 8774.0 22 9184.0 23 10087.0 24 11333.0 25 12816.0 26 14898.0 27 17154.0 28 20123.0 29 23996.0 30 28755.0 31 35323.0 32 43668.0 33 57492.0 34 79407.0 35 120446.0 36 186948.0 37 287684.0 38 267782.0 39 44068.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.15909701149956 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20.469238069590038 28.814260470836615 30.271349590498588 20.44515186907476 20-21 22.521606781953246 26.275748014314864 29.12428215938555 22.07836304434634 22-23 21.434338152895048 26.48742391113339 29.53753237287794 22.54070556309362 24-25 22.557417811073243 26.52933187496601 29.598962191815158 21.314288122145587 26-27 22.221842066784266 25.5050164911429 31.31901771996534 20.954123722107497 28-29 20.649671023015784 27.789281868632486 30.63101638942808 20.93003071892365 30-31 21.048240329500253 26.886729606266208 30.037334527884585 22.02769553634895 32-33 21.152127527039223 27.094562918442694 29.983595353985326 21.769714200532757 34-35 22.509631637674318 26.35683756057214 29.5923316430152 21.54119915873834 36-37 22.350814985099117 25.67419030114418 30.69971788629788 21.275276827458825 38-39 21.898692506966032 26.618900271992374 30.531478229950093 20.9509289910915 40-41 21.270621727122144 27.182945179126563 30.420247000106578 21.126186093644726 42-43 20.81850565960275 27.62723128858101 28.841802529537592 22.712460522278644 44-45 21.5125367065733 26.813535125367068 28.73895640388525 22.934971764174385 46-47 22.44324804953292 27.562914649331592 28.333559238716933 21.660278062418563 48-49 22.336278835222394 27.045165768074714 28.59608186193796 22.022473534764934 50-51 21.018815672052057 27.70136658229367 29.59352408642407 21.686293659230206 52-53 21.6057535629033 28.517070272005274 27.951097462411855 21.92607870267957 54-55 20.64000387429923 27.139213112479005 28.811703274939354 23.40907973828241 56-57 21.981309262191363 27.415996826101125 27.873834629173423 22.72885928253409 58-59 22.89571935097565 26.679286383891075 27.92979439373879 22.495199871394483 60-61 21.739369079865103 27.06606862418331 29.603283480009257 21.591278815942328 62-63 21.61509563779489 27.06134752951958 29.16685336983561 22.156703462849926 64-65 21.016441857060492 28.252955539931644 28.77080987487416 21.959792728133703 66-67 21.35283021459873 27.239925297865003 27.970425705285184 23.436818782251084 68-69 20.70350886707522 27.61516351212105 28.62353345264566 23.057794168158072 70-71 21.97753848879184 27.421842942953255 27.905815662321746 22.69480290593316 72-73 21.566191977593192 26.46110222238931 29.011221479072063 22.961484320945434 74-75 20.586815787390176 28.70642616756342 28.10459997375348 22.602158071292923 76-77 21.248547475634638 26.912046148257783 28.245133704528985 23.594272671578594 78-79 21.21951924605756 26.99297515285547 28.809931630602893 22.977573970484077 80-81 21.706847735941444 27.484718666264346 28.215693112152252 22.59274048564196 82-83 21.230702507341313 26.901901965213465 28.807156087644003 23.06023943980122 84-85 20.954716387864377 26.997771870634775 28.49493746600431 23.55257427549654 86-87 21.0810531436655 27.229523471502475 28.426284208266438 23.26313917656558 88-89 21.13519410421724 26.809104224472357 28.02195736633059 24.03374430497981 90-91 21.148126362734846 26.029369816817344 28.623044449513873 24.19945937093394 92-93 21.601095203459998 26.908219231564534 28.60250216539105 22.88818339958442 94-95 21.141851512161896 26.3920938174112 28.667575996723233 23.79847867370367 96-97 20.155321245722373 26.16424054042134 29.387290149659922 24.293148064196362 98-99 21.021775271906222 26.654274676346013 27.9229354123536 24.401014639394166 100-101 20.997170222894194 25.32692076226511 29.004004483402124 24.671904531438575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2914.0 1 1766.5 2 758.0 3 914.0 4 788.5 5 770.5 6 1368.5 7 2592.5 8 3704.0 9 4019.5 10 3441.5 11 2838.5 12 2822.5 13 2867.0 14 2904.0 15 3075.5 16 3260.5 17 3299.5 18 3288.0 19 3378.0 20 3538.0 21 3818.5 22 4332.0 23 4990.5 24 5796.5 25 6712.5 26 8171.5 27 9837.5 28 10884.0 29 12270.0 30 14301.0 31 16703.0 32 19676.0 33 22700.0 34 26179.5 35 28910.0 36 31934.5 37 35682.5 38 36960.5 39 36445.5 40 35827.0 41 36761.5 42 38002.5 43 39485.5 44 41254.0 45 43208.5 46 47413.0 47 61912.0 48 100746.0 49 100325.0 50 61557.0 51 45692.0 52 39692.0 53 38088.5 54 37015.5 55 33995.0 56 30203.0 57 26707.5 58 23904.5 59 21339.5 60 18738.5 61 16456.5 62 14127.5 63 11174.5 64 8422.5 65 6525.5 66 5013.0 67 4236.5 68 3345.0 69 2289.0 70 1720.0 71 1311.0 72 1081.0 73 926.5 74 722.0 75 561.5 76 387.5 77 229.0 78 136.5 79 101.0 80 70.5 81 50.5 82 41.0 83 31.5 84 24.5 85 15.0 86 9.0 87 9.5 88 8.5 89 6.5 90 4.5 91 2.5 92 3.5 93 4.0 94 2.0 95 1.5 96 1.5 97 3.0 98 3.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33393617373313594 2 0.11805025012840932 3 0.1110003265984982 4 0.11826606411401884 5 0.13696994286684422 6 0.08352001243088557 7 0.09344745576892365 8 0.09841117743794268 9 0.08690109820543478 10-11 0.08531846231096493 12-13 0.08891536207112366 14-15 0.0907497809488046 16-17 0.08028280264674272 18-19 0.09524590564900301 20-21 0.10499350399903315 22-23 0.11351815643060932 24-25 0.12772591048323628 26-27 0.12697056153360295 28-29 0.12164714988856803 30-31 0.11215133452174902 32-33 0.10466978302061886 34-35 0.3271740021840375 36-37 0.4876317004847182 38-39 0.4609427042643405 40-41 0.43727510384249607 42-43 0.47137371356880076 44-45 0.4782797611083055 46-47 0.4865526305566706 48-49 0.49662394988511505 50-51 0.4851858086478103 52-53 0.48946611936239914 54-55 0.47551014829298327 56-57 0.453137431784796 58-59 0.4322394441782738 60-61 0.3926016088213248 62-63 0.46241743316600553 64-65 0.49482550000503567 66-67 0.5030624004557991 68-69 0.5151479836499324 70-71 0.5074146491655912 72-73 0.498889996734015 74-75 0.5068391452039658 76-77 0.4833154207725277 78-79 0.46227355717559915 80-81 0.4642518520436866 82-83 0.4782797611083055 84-85 0.4775603811562738 86-87 0.48090549793322146 88-89 0.49349464709377694 90-91 0.5084217810984356 92-93 0.500976198594907 94-95 0.5047529433430737 96-97 0.5047889123406754 98-99 0.4988540277364134 100-101 0.4864087545662642 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1390086.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.64132700024418 #Duplication Level Percentage of deduplicated Percentage of total 1 68.70968346943532 25.863236635562874 2 14.36336068539847 10.813119127630706 3 6.091483966118562 6.878746196562395 4 3.192365634222458 4.8065951496843775 5 1.8736895689867523 3.5264080881588464 6 1.200895618186589 2.7121982794393076 7 0.8401935742746719 2.2138200750943815 8 0.6056342322544478 1.8237500943065204 9 0.4422533575208584 1.498230292265868 >10 2.3810895859198298 16.491530406784477 >50 0.19428559520686053 5.064003632031008 >100 0.09219573113140207 5.915244057841392 >500 0.004993933058785122 1.3790374885219594 >1k 0.006338453497688808 5.972792544918534 >5k 0.0013445204389036867 3.765204693679701 >10k+ 1.920743484148124E-4 1.276083237517699 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17650 1.2697056153360295 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9896 0.7118984005306146 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8523 0.613127533116656 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8127 0.584640087016199 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7359 0.529391706700161 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6685 0.48090549793322146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6431 0.46263324715161513 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5057 0.36379044174245334 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4877 0.35084160260588193 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4639 0.3337203597475264 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4478 0.3221383425198153 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4385 0.31544810896592007 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4330 0.3114915192297455 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4142 0.2979671761315487 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3900 0.28055818129238047 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3866 0.27811228945547256 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3523 0.2534375571007837 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2896 0.2083324341083933 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2681 0.19286576513971077 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2510 0.18056436795996794 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2499 0.17977305001273303 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2477 0.1781904141182632 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2462 0.17711134419021557 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2291 0.16480994701047275 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2198 0.15811971345657752 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2148 0.1545228136964188 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2107 0.15157335589308865 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1859 0.13373273308270134 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1788 0.12862513542327597 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1756 0.1263231195767744 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1676 0.12056807996052044 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1527 0.10984931867524743 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1501 0.10797893079996489 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1408 0.10128869724606968 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.193799520317448E-5 0.0 12-13 0.0 0.0 0.0 7.193799520317448E-5 0.0 14-15 0.0 0.0 0.0 7.193799520317448E-5 0.0 16-17 0.0 0.0 0.0 2.1581398560952344E-4 0.0 18-19 0.0 0.0 0.0 4.316279712190469E-4 0.0 20-21 0.0 0.0 0.0 7.913179472349192E-4 0.0 22-23 0.0 0.0 0.0 0.0019063568728841236 0.0 24-25 0.0 0.0 0.0 0.0038486827433698344 0.0 26-27 0.0 0.0 0.0 0.006330543577879355 0.0 28-29 0.0 0.0 0.0 0.014063878062220612 0.0 30-31 0.0 0.0 0.0 0.0326958188198428 0.0 32-33 0.0 0.0 0.0 0.06837706444061735 0.0 34-35 0.0 0.0 0.0 0.12161118089096645 0.0 36-37 0.0 0.0 0.0 0.20570669728347743 0.0 38-39 0.0 0.0 0.0 0.3276415991528582 0.0 40-41 0.0 0.0 0.0 0.46090673526673887 0.0 42-43 0.0 0.0 0.0 0.5909346615964768 0.0 44-45 0.0 0.0 0.0 0.7455294132880987 0.0 46-47 0.0 0.0 0.0 0.9374599844901683 0.0 48-49 0.0 0.0 0.0 1.1436342787424663 0.0 50-51 0.0 0.0 0.0 1.3622898151625151 0.0 52-53 0.0 0.0 0.0 1.5809813205801655 0.0 54-55 0.0 0.0 0.0 1.8274049231486398 0.0 56-57 0.0 0.0 0.0 2.131091169898841 0.0 58-59 0.0 0.0 0.0 2.4561070322267833 0.0 60-61 0.0 0.0 0.0 2.8295731343240638 0.0 62-63 0.0 0.0 0.0 3.1882200094094895 0.0 64-65 0.0 0.0 0.0 3.552226265137553 0.0 66-67 0.0 0.0 0.0 3.9703658622560045 0.0 68-69 0.0 0.0 0.0 4.44706298746984 0.0 70-71 0.0 0.0 0.0 4.974728182285125 0.0 72-73 0.0 0.0 0.0 5.493616941685622 0.0 74-75 0.0 0.0 0.0 6.04692803179084 0.0 76-77 0.0 0.0 0.0 6.610382379219702 0.0 78-79 0.0 0.0 0.0 7.295735659520346 0.0 80-81 0.0 0.0 0.0 8.051696082112905 0.0 82-83 0.0 0.0 0.0 8.770068902211806 0.0 84-85 0.0 0.0 0.0 9.55106374713507 0.0 86-87 0.0 0.0 0.0 10.373315032307353 0.0 88-89 0.0 0.0 0.0 11.212975312318807 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10225 0.0 25.026608 1 TACATGG 10375 0.0 24.109192 2 CCCTATA 240 2.382876E-10 23.686825 2 TATCTCG 120 1.7422435E-4 23.686825 5 ACGTATA 120 1.7460767E-4 23.67915 9 ATGGGAG 1965 0.0 23.385464 5 ACATGGG 10430 0.0 23.30075 3 GAGTACT 6240 0.0 23.261728 12-13 CATGGGG 2635 0.0 22.83284 4 AGTACTT 6450 0.0 22.173964 12-13 ATGGGCG 130 2.995395E-4 21.864761 5 CATGGGA 7315 0.0 21.824907 4 GTACTTT 6790 0.0 21.413523 14-15 ATGGGAT 2410 0.0 21.033113 5 ATGGGGA 1370 0.0 20.401789 5 GTTCGCA 125 0.00613674 18.943317 9 ACTTTTT 7895 0.0 18.507427 16-17 TACTTTT 7590 0.0 18.501308 14-15 TAGGACA 515 0.0 18.397535 4 GCCGTAT 170 1.724402E-8 16.786629 92-93 >>END_MODULE