##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939124_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1369904 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09397738819654 33.0 31.0 34.0 30.0 34.0 2 32.17233543372382 34.0 31.0 34.0 30.0 34.0 3 32.2300883857555 34.0 31.0 34.0 30.0 34.0 4 35.67763653511487 37.0 35.0 37.0 35.0 37.0 5 35.73169798759621 37.0 35.0 37.0 35.0 37.0 6 35.84422339083615 37.0 36.0 37.0 35.0 37.0 7 35.783176777350825 37.0 36.0 37.0 35.0 37.0 8 35.79157882596153 37.0 36.0 37.0 35.0 37.0 9 37.438406632873544 39.0 38.0 39.0 35.0 39.0 10-11 37.340486267650874 39.0 37.0 39.0 34.0 39.0 12-13 37.2607357887852 39.0 37.0 39.0 34.0 39.0 14-15 38.46043664373562 40.0 38.0 41.0 34.0 41.0 16-17 38.41556744122216 40.0 38.0 41.0 34.0 41.0 18-19 38.37467698466462 40.0 38.0 41.0 34.0 41.0 20-21 38.370025928824205 40.0 38.0 41.0 34.0 41.0 22-23 38.40401298193158 40.0 38.0 41.0 34.0 41.0 24-25 38.33608340438454 40.0 38.0 41.0 34.0 41.0 26-27 38.1888281952604 40.0 38.0 41.0 34.0 41.0 28-29 38.057777406300005 40.0 38.0 41.0 33.0 41.0 30-31 37.86283089909949 40.0 37.5 41.0 33.0 41.0 32-33 37.63167090540651 40.0 37.0 41.0 33.0 41.0 34-35 37.28143395449608 40.0 37.0 41.0 31.5 41.0 36-37 36.98545518518086 40.0 36.0 41.0 31.0 41.0 38-39 36.77262202314907 40.0 36.0 41.0 30.5 41.0 40-41 36.59598300318855 40.0 35.5 41.0 30.0 41.0 42-43 36.36371015779208 39.0 35.0 41.0 30.0 41.0 44-45 36.03461665926956 39.0 35.0 41.0 29.5 41.0 46-47 35.76331078674126 39.0 35.0 41.0 27.5 41.0 48-49 35.56385009460517 39.0 35.0 41.0 27.0 41.0 50-51 34.39192709854121 38.0 34.0 40.0 24.5 40.5 52-53 34.54896036510588 38.5 34.5 39.5 24.5 40.5 54-55 35.09221777584415 39.0 35.0 40.5 26.0 41.0 56-57 34.99336413354513 39.0 35.0 41.0 25.0 41.0 58-59 34.80417204417244 39.0 35.0 41.0 25.0 41.0 60-61 34.451005690909724 38.0 34.5 40.0 23.0 41.0 62-63 34.08790323993506 38.0 34.0 40.0 22.0 41.0 64-65 33.68540240775996 37.0 34.0 40.0 22.0 41.0 66-67 33.249239727747344 36.5 33.5 39.0 20.0 41.0 68-69 32.8149352071386 36.0 33.0 39.0 18.5 41.0 70-71 32.30801720412525 35.5 32.5 38.5 15.0 40.0 72-73 31.830522065779792 35.0 32.0 37.5 11.5 40.0 74-75 31.389743003889322 35.0 32.0 37.0 7.5 39.0 76-77 30.93980563601537 35.0 31.0 36.5 4.5 39.0 78-79 30.487873967810884 35.0 31.0 36.0 2.0 38.5 80-81 30.0147933723823 34.5 30.5 35.5 2.0 37.0 82-83 29.622844374496317 34.0 30.0 35.0 2.0 37.0 84-85 29.239964990247493 34.0 30.0 35.0 2.0 36.5 86-87 28.84998912332543 34.0 29.0 35.0 2.0 36.0 88-89 28.381900483537535 34.0 29.0 35.0 2.0 36.0 90-91 27.912916160548477 34.0 28.0 35.0 2.0 35.0 92-93 27.458064944696858 34.0 27.0 35.0 2.0 35.0 94-95 26.950266588023688 34.0 25.5 35.0 2.0 35.0 96-97 26.29048166878847 33.0 24.5 35.0 2.0 35.0 98-99 25.550133075018394 33.0 21.0 35.0 2.0 35.0 100-101 22.704339501162124 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2976174829809324 1101 2 0.4198179227983516 1101 3 0.30410348541565213 1101 4 0.3222927913216864 1101 5 0.2115977771694162 1101 6 0.32174922478707657 1101 7 0.23188579300808243 1101 8 0.28448092468969577 1101 9 0.35793565377376524 1101 10-11 0.3426545078876586 1101 12-13 0.40196382452806034 1101 14-15 0.6143438576727931 1101 16-17 0.4989726610402059 1101 18-19 0.6296023530431967 1101 20-21 0.5407599860808503 1101 22-23 0.6224014142571761 1101 24-25 0.5736256649749478 1101 26-27 0.4684655681871135 1101 28-29 0.41796001578640585 1101 30-31 0.5156059286200616 1101 32-33 0.5995871653077245 1101 34-35 0.7407060671467676 1101 36-37 0.6963253775715046 1101 38-39 0.5869727668019635 1101 40-41 0.39505825884184276 1101 42-43 0.465700111121258 1101 44-45 0.3702032246846372 1101 46-47 0.6355247432590616 1101 48-49 0.8354990064792105 1101 50-51 0.8795088016390551 1101 52-53 0.7067128155005022 1101 54-55 0.7818546020130483 1101 56-57 0.8786545738728648 1101 58-59 0.8370808105834371 1101 60-61 0.675521311123326 1101 62-63 0.5632535766288598 1101 64-65 0.506283245699997 1101 66-67 0.6429739286352003 1101 68-69 0.6297093490229528 1101 70-71 0.4525098608057405 1101 72-73 0.43640053611672514 1101 74-75 0.3618871389834091 1101 76-77 0.36240464952104645 1101 78-79 0.4560685184996167 1101 80-81 0.2673466209922175 1101 82-83 0.5041428365505709 1101 84-85 0.48219321748532096 1101 86-87 0.3084891725273735 1101 88-89 0.28166294886017695 1101 90-91 0.19629231565226135 1101 92-93 0.27829362467606344 1101 94-95 -0.022751380449264502 1101 96-97 -0.24336774308029874 1101 98-99 -0.5309803078054429 1101 100-101 -0.7102130614984077 1102 1 0.12753488867966922 1102 2 0.24830467032495562 1102 3 0.4322591619913183 1102 4 0.18649357679619527 1102 5 0.2958154437936926 1102 6 0.18305940242456842 1102 7 0.2910638583049874 1102 8 0.3134995440583097 1102 9 0.33814693793593165 1102 10-11 0.3152665767535012 1102 12-13 0.5041799572031067 1102 14-15 0.38857505234174283 1102 16-17 0.4877162487084874 1102 18-19 0.5626450839353154 1102 20-21 0.5938727853069352 1102 22-23 0.5561093703661868 1102 24-25 0.6083133444335189 1102 26-27 0.5339116275178029 1102 28-29 0.594398093185319 1102 30-31 0.6275645659489868 1102 32-33 0.5004146499778699 1102 34-35 0.4368935481169345 1102 36-37 0.33769267361984845 1102 38-39 0.36316764772819 1102 40-41 0.39992835492564893 1102 42-43 0.34138624506817195 1102 44-45 0.35383005106702115 1102 46-47 0.6445480646831001 1102 48-49 0.3725681613811034 1102 50-51 0.03642641160741533 1102 52-53 0.002038895335239488 1102 54-55 0.02778558971447609 1102 56-57 0.17540938480784263 1102 58-59 -0.012867351182691777 1102 60-61 -0.009035520153823029 1102 62-63 -0.03431330240113084 1102 64-65 -0.15020038767843147 1102 66-67 0.04198919489871855 1102 68-69 -0.06255075943265354 1102 70-71 -0.2883517715783128 1102 72-73 -0.3692307533641639 1102 74-75 -0.33127445370296726 1102 76-77 -0.35161287141658804 1102 78-79 -0.28431057508285207 1102 80-81 -0.3424479146027295 1102 82-83 -0.6583998962686195 1102 84-85 -0.4023566763330173 1102 86-87 -0.5344228643490752 1102 88-89 -0.5004904383540847 1102 90-91 -0.509397171399744 1102 92-93 -0.6560911190811858 1102 94-95 -0.7541965687434597 1102 96-97 -0.7006125698558066 1102 98-99 -0.9066413898177679 1102 100-101 -1.0603203994027588 1103 1 0.3160396575020741 1103 2 0.3042932150505777 1103 3 0.3658313449251622 1103 4 0.32554028763814813 1103 5 0.41737176877768434 1103 6 0.3521539277481196 1103 7 0.3737090879229328 1103 8 0.4997012264031966 1103 9 0.39564834533640436 1103 10-11 0.4252532528672077 1103 12-13 0.46693843242765354 1103 14-15 0.47328793521779744 1103 16-17 0.35739356408650735 1103 18-19 0.42768354793037133 1103 20-21 0.6032537221974721 1103 22-23 0.5509680881538017 1103 24-25 0.5929767640527572 1103 26-27 0.530340395229139 1103 28-29 0.37527697950449834 1103 30-31 0.48760880065734113 1103 32-33 0.43561964027089317 1103 34-35 0.4290993717098601 1103 36-37 0.2801073420749205 1103 38-39 0.4708545794763239 1103 40-41 0.4096338480208459 1103 42-43 0.3323276363076815 1103 44-45 0.4114038708930252 1103 46-47 0.3882292450279081 1103 48-49 0.4182570597764439 1103 50-51 0.17517344641142785 1103 52-53 0.2414094939594733 1103 54-55 0.28246603182389407 1103 56-57 0.26126304189527616 1103 58-59 0.334523276347106 1103 60-61 0.16679001863175102 1103 62-63 0.21167096442120226 1103 64-65 0.2605262320204247 1103 66-67 0.17360861895627977 1103 68-69 0.04424754188324442 1103 70-71 0.15421737671787383 1103 72-73 0.10485636732469672 1103 74-75 -0.24827830233054726 1103 76-77 -0.19455069179515405 1103 78-79 -0.08058174364857962 1103 80-81 0.0713267111921354 1103 82-83 -0.07176047152778153 1103 84-85 0.17449555924279636 1103 86-87 0.07437587664021805 1103 88-89 0.014201485766605515 1103 90-91 0.21248155598344098 1103 92-93 -0.0249826668058013 1103 94-95 -0.2952789228379942 1103 96-97 -0.2178081071708604 1103 98-99 -0.29884602510423264 1103 100-101 -0.1630383533766775 1104 1 0.3830872303512507 1104 2 0.43067830219239767 1104 3 0.45526414351148503 1104 4 0.4925310363994555 1104 5 0.42215607978562275 1104 6 0.45307350780424827 1104 7 0.48654370903793165 1104 8 0.5339855547355512 1104 9 0.6693983886474513 1104 10-11 0.5995528266865477 1104 12-13 0.6397162294547911 1104 14-15 0.6152788572232737 1104 16-17 0.6630596879214465 1104 18-19 0.8006335496628125 1104 20-21 0.7585879102020456 1104 22-23 0.798170541291249 1104 24-25 0.8076380716997633 1104 26-27 0.7084080297532367 1104 28-29 0.7451217527278757 1104 30-31 0.8178697063778628 1104 32-33 0.6040895477585053 1104 34-35 0.7868087372897108 1104 36-37 0.8725961678259324 1104 38-39 0.3647449505653384 1104 40-41 0.5061793592643937 1104 42-43 0.5920095600109434 1104 44-45 0.530375839986263 1104 46-47 0.7706501590641537 1104 48-49 0.6046116559442822 1104 50-51 0.6193164075007829 1104 52-53 0.6965285127044964 1104 54-55 0.7089893678136363 1104 56-57 0.7278553290648091 1104 58-59 0.7515535247792471 1104 60-61 0.68262730770698 1104 62-63 0.7623653270558961 1104 64-65 0.6520514736960408 1104 66-67 0.6866168307299603 1104 68-69 0.6425871697547691 1104 70-71 0.6966490318926759 1104 72-73 0.7561667862291053 1104 74-75 0.5792817267744752 1104 76-77 0.6109778761685334 1104 78-79 0.7060891963742897 1104 80-81 0.5632074499052777 1104 82-83 0.5584519258250467 1104 84-85 0.3071271381901646 1104 86-87 0.3674319038049063 1104 88-89 0.3095079547039248 1104 90-91 0.43024358779107175 1104 92-93 0.45786927872623195 1104 94-95 0.47002754609932396 1104 96-97 0.6365531415731205 1104 98-99 0.6842997425406878 1104 100-101 0.4196296068464598 1105 1 0.1699222630140369 1105 2 0.26245927510579037 1105 3 0.20717738855533696 1105 4 0.21237477373553304 1105 5 0.1798601208937214 1105 6 0.15844641471125698 1105 7 0.16788912409037238 1105 8 0.2424700986722641 1105 9 0.3169612422241457 1105 10-11 0.18898629348073115 1105 12-13 0.20709656045931268 1105 14-15 0.26221276978751007 1105 16-17 0.2813998023627704 1105 18-19 0.3383009565779176 1105 20-21 0.3721522875018124 1105 22-23 0.31901539710489857 1105 24-25 0.3177037214703873 1105 26-27 0.3784934555041559 1105 28-29 0.3241277665710598 1105 30-31 0.49357669767319123 1105 32-33 0.4419303997466386 1105 34-35 0.4474221168750674 1105 36-37 0.342301311201787 1105 38-39 0.3627524395021311 1105 40-41 0.34802176739523816 1105 42-43 0.26879255915182654 1105 44-45 0.33162075415526715 1105 46-47 0.43247603317869476 1105 48-49 0.2395781826661718 1105 50-51 0.19208293746464022 1105 52-53 0.20484188094297195 1105 54-55 0.20503676532705128 1105 56-57 0.1945454459376066 1105 58-59 0.2337395810921663 1105 60-61 0.3514934308043465 1105 62-63 0.22406087421837384 1105 64-65 0.27646411087164324 1105 66-67 0.24575706188198865 1105 68-69 0.37817939552514446 1105 70-71 0.2771920608596261 1105 72-73 0.18920693795301347 1105 74-75 0.03115055691176849 1105 76-77 0.09263387137042756 1105 78-79 0.09770774689219408 1105 80-81 0.23583360282871624 1105 82-83 0.15748808993326335 1105 84-85 -0.048091289133108006 1105 86-87 -0.0659755624381475 1105 88-89 0.060637941249773064 1105 90-91 0.15094061004715442 1105 92-93 -0.02315777546319353 1105 94-95 -0.19344135536118046 1105 96-97 -0.15224619706622633 1105 98-99 -0.08808877442288932 1105 100-101 -0.5518942783231751 1106 1 0.15372453515804807 1106 2 0.20352275847069023 1106 3 0.4139428598176593 1106 4 0.2780959024072587 1106 5 0.218870725736096 1106 6 0.2741883604989752 1106 7 0.22532485772150324 1106 8 0.17514394902188712 1106 9 0.17989379172041708 1106 10-11 0.25179302591282493 1106 12-13 0.3232944711889303 1106 14-15 0.3153195795751458 1106 16-17 0.3545462103892163 1106 18-19 0.37198506187169045 1106 20-21 0.43167826683586696 1106 22-23 0.4637614069544753 1106 24-25 0.43211251842395626 1106 26-27 0.5010072121737039 1106 28-29 0.4160744991372596 1106 30-31 0.5074272423623256 1106 32-33 0.4265726714761442 1106 34-35 0.29718197330706886 1106 36-37 0.2782434182840561 1106 38-39 0.3233547521221851 1106 40-41 0.5051096144055833 1106 42-43 0.47805181728224255 1106 44-45 0.4346657831315923 1106 46-47 0.28025750161744867 1106 48-49 0.2854383196872945 1106 50-51 0.5180302076793382 1106 52-53 0.41562447649292267 1106 54-55 0.368640095334122 1106 56-57 0.3264717088477269 1106 58-59 0.44697664567764406 1106 60-61 0.5639191460079118 1106 62-63 0.6231938702920772 1106 64-65 0.49509625262066237 1106 66-67 0.5639742633422955 1106 68-69 0.4817282413791233 1106 70-71 0.3056167202727167 1106 72-73 0.4248365749427485 1106 74-75 0.4870155278927726 1106 76-77 0.4325792043436998 1106 78-79 0.3825310025672195 1106 80-81 0.32344927802563106 1106 82-83 0.48801604003558197 1106 84-85 0.40172422184327417 1106 86-87 0.5073244594457336 1106 88-89 0.5203216186192208 1106 90-91 0.37306533352697 1106 92-93 0.4029774278953404 1106 94-95 0.5191681478503867 1106 96-97 0.6977315837865383 1106 98-99 0.48437429576949853 1106 100-101 0.5291373362620959 1107 1 0.19526073853779025 1107 2 0.2514589338366946 1107 3 0.25037239213413187 1107 4 0.2706454152308382 1107 5 0.19096495694865467 1107 6 0.2333419954274376 1107 7 0.2546324806682563 1107 8 0.28545913275991097 1107 9 0.288797783386336 1107 10-11 0.23877685089699696 1107 12-13 0.3186888375094199 1107 14-15 0.38186533972502446 1107 16-17 0.38833632329041734 1107 18-19 0.3748696014979984 1107 20-21 0.440135205926417 1107 22-23 0.4418952250811188 1107 24-25 0.4906069550705965 1107 26-27 0.4554306106487118 1107 28-29 0.6548773203541671 1107 30-31 0.5673521305262312 1107 32-33 0.5897246839422579 1107 34-35 0.6950418404168985 1107 36-37 0.8175143023801326 1107 38-39 0.8619095350302999 1107 40-41 0.7617857843439282 1107 42-43 0.6211815938434952 1107 44-45 0.6017035345621977 1107 46-47 0.7108534647210192 1107 48-49 0.6988647792468399 1107 50-51 0.6890362802407495 1107 52-53 0.4911603121923491 1107 54-55 0.42024256871376764 1107 56-57 0.4418042698578546 1107 58-59 0.5427524073741097 1107 60-61 0.5724108104616974 1107 62-63 0.4144503740024348 1107 64-65 0.55125066668122 1107 66-67 0.5337915028801845 1107 68-69 0.4388733496259576 1107 70-71 0.48607713773577643 1107 72-73 0.5576380363261002 1107 74-75 0.5942953063507872 1107 76-77 0.5327502048718777 1107 78-79 0.5131412643497697 1107 80-81 0.48130786117244995 1107 82-83 0.5463917101037765 1107 84-85 0.5674313206996722 1107 86-87 0.5042379874550917 1107 88-89 0.5898152411165896 1107 90-91 0.7047946857968093 1107 92-93 0.774461193058503 1107 94-95 0.863034452283852 1107 96-97 0.9557243994298759 1107 98-99 0.8815186453109192 1107 100-101 0.6518512255319564 1108 1 0.07698485497486729 1108 2 0.2518589950358532 1108 3 0.25201343637318985 1108 4 0.280076886334065 1108 5 0.3664025593014202 1108 6 0.24917557903512488 1108 7 0.12882966664005124 1108 8 0.2729155708552611 1108 9 0.20349918246721188 1108 10-11 0.291097885842845 1108 12-13 0.3023866269729609 1108 14-15 0.2590788511403659 1108 16-17 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-2.263672586901393 2316 84-85 -2.1183784797873635 2316 86-87 -2.156837759116442 2316 88-89 -2.6085303392653465 2316 90-91 -2.690188652520785 2316 92-93 -2.7789491115512277 2316 94-95 -2.7612691748362366 2316 96-97 -2.8093010203163544 2316 98-99 -3.0086135800917333 2316 100-101 -2.7657877286270534 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4094.0 3 943.0 4 959.0 5 1061.0 6 1148.0 7 1504.0 8 1842.0 9 2394.0 10 2994.0 11 3953.0 12 5595.0 13 9113.0 14 7668.0 15 8465.0 16 11149.0 17 16893.0 18 13402.0 19 8315.0 20 8322.0 21 8502.0 22 9083.0 23 10410.0 24 11404.0 25 12903.0 26 15004.0 27 17131.0 28 19984.0 29 23795.0 30 28535.0 31 34939.0 32 43576.0 33 56601.0 34 79114.0 35 120167.0 36 185281.0 37 284269.0 38 259428.0 39 39961.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.18074452189958 19.360339307710852 14.265081217972817 21.193834952416754 2 14.909447270994624 22.09768674353649 39.230137374723576 23.762728610745306 3 21.42019851530082 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22-23 21.418217366621782 26.55925440733903 29.484146438583657 22.538381787455535 24-25 22.58798779246905 26.616633765668162 29.52193259844085 21.273445843421936 26-27 22.252275688406247 25.479087488591418 31.33719940342875 20.931437419573587 28-29 20.616569905189465 27.747472455768662 30.693062072387157 20.94289556665472 30-31 21.08485010550188 26.912331153155993 29.975392636494885 22.02742610484724 32-33 21.201750979656534 27.166377040730406 29.975973893847495 21.655898085765564 34-35 22.459886585649443 26.338239898633866 29.636154898295125 21.565718617421574 36-37 22.291150256755706 25.66705851447122 30.716575162132532 21.325216066640543 38-39 21.884226979711606 26.65392780225976 30.494735772499386 20.96710944552925 40-41 21.301589397173267 27.167286436215075 30.364490505423902 21.166633661187756 42-43 20.840439092834938 27.633465697018604 28.77837183496419 22.747723375182268 44-45 21.441437938037826 26.775080416994403 28.819880607177655 22.96360103779012 46-47 22.392117573126434 27.572242311695277 28.34183401134541 21.69380610383288 48-49 22.318063116310025 27.01931762066084 28.562115098591264 22.10050416443788 50-51 21.05605320622696 27.727927105590645 29.549626957665126 21.66639273051727 52-53 21.627766200986148 28.519862350316977 27.949709438835406 21.90266200986147 54-55 20.636142294137304 27.158831239675546 28.748489965446666 23.45653650074048 56-57 21.988141479260058 27.382679166023326 27.854980861108903 22.774198493607717 58-59 22.82787469888001 26.689324849472097 27.988087585073618 22.49471286657428 60-61 21.78617336060782 27.040922357515427 29.54191839426048 21.630985887616266 62-63 21.62652876104653 27.03743412797302 29.11804934803795 22.2179877629425 64-65 21.0536395726269 28.210902556188937 28.76649052073668 21.968967350447482 66-67 21.38023349296061 27.1914320111459 27.94855772212085 23.47977677377264 68-69 20.719225967265672 27.56746619808973 28.60029520934394 23.11301262530066 70-71 21.975774563328194 27.44420432097474 27.904867069090606 22.675154046606462 72-73 21.616736608009383 26.379597112915302 29.002791905272623 23.00087437380269 74-75 20.64008571659218 28.6821073952577 28.02719740501824 22.650609483131884 76-77 21.339690030316103 26.885548639297063 28.1930223071347 23.58173902325213 78-79 21.28188709083521 26.95969269272353 28.771511729402942 22.986908487038317 80-81 21.689318429811934 27.485370035852814 28.201692692812014 22.62361884152324 82-83 21.197424714799745 26.917937086652678 28.815799464943453 23.068838733604128 84-85 20.962748639516168 26.97044569875829 28.455020034949285 23.611785626776257 86-87 21.151497189171028 27.197658901887657 28.397699103747943 23.253144805193376 88-89 21.14089023349214 26.783601660920063 27.987471565052886 24.08803654053491 90-91 21.13000386726263 26.046087203470115 28.63744669671928 24.186462232547967 92-93 21.579602446438294 26.857872326684934 28.584308038695642 22.978217188181127 94-95 21.11666711920328 26.357151191504446 28.677416490483154 23.84876519880912 96-97 20.21977304257754 26.117202260107575 29.326873506846994 24.33615119046789 98-99 21.010425461418308 26.643359542839868 27.910464245470397 24.435750750271435 100-101 21.071849774489323 25.219852276291387 29.015878372801524 24.692419576417766 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2725.0 1 1674.0 2 775.5 3 884.5 4 728.0 5 745.0 6 1320.5 7 2488.0 8 3639.0 9 3922.0 10 3289.5 11 2771.0 12 2757.5 13 2872.0 14 2939.5 15 3091.0 16 3312.0 17 3328.5 18 3255.0 19 3378.5 20 3545.0 21 3794.5 22 4252.0 23 4791.0 24 5676.0 25 6672.5 26 8062.5 27 9743.5 28 10800.5 29 12086.0 30 14183.0 31 16389.5 32 19186.0 33 22311.5 34 25746.0 35 28632.5 36 31595.0 37 35216.5 38 36434.5 39 35835.0 40 35421.5 41 36338.0 42 37276.0 43 38679.5 44 40540.5 45 42493.5 46 46579.5 47 60751.0 48 98939.5 49 98577.0 50 60972.5 51 45342.5 52 39019.0 53 37569.5 54 36590.0 55 33606.0 56 29814.0 57 26483.5 58 23701.0 59 20896.5 60 18347.5 61 16234.0 62 14046.0 63 11119.0 64 8324.5 65 6563.0 66 5056.5 67 4190.0 68 3324.5 69 2265.0 70 1686.0 71 1319.5 72 1067.0 73 875.0 74 679.5 75 558.5 76 402.0 77 253.0 78 148.0 79 100.0 80 74.0 81 52.5 82 39.5 83 26.0 84 17.5 85 13.0 86 10.5 87 7.5 88 7.5 89 6.5 90 3.5 91 1.5 92 1.5 93 0.5 94 2.0 95 3.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38272754879174015 2 0.1116866583351826 3 0.11285462338966817 4 0.12606722806853618 5 0.12781917565026454 6 0.06832595568740583 7 0.077377684859669 8 0.07474976348707646 9 0.07416578095983367 10-11 0.07241383337810534 12-13 0.07606372417337273 14-15 0.07051589016456628 16-17 0.07208534320653126 18-19 0.0810275756549364 20-21 0.07923912916525537 22-23 0.08913033322043006 24-25 0.10252543243906141 26-27 0.10343790513787827 28-29 0.09726958969387636 30-31 0.08818136161366052 32-33 0.08646591293988483 34-35 0.3326145481727187 36-37 0.5204744274051321 38-39 0.5024804657844637 40-41 0.4740478164893307 42-43 0.5012395029140728 44-45 0.5115686938646796 46-47 0.5090502692159451 48-49 0.5149995912122309 50-51 0.5150725890281362 52-53 0.5127366589191652 54-55 0.5064953456592578 56-57 0.48036212756514324 58-59 0.47101840712925874 60-61 0.41955494691598827 62-63 0.4926987584531471 64-65 0.5192699634426938 66-67 0.5292706642217265 68-69 0.5460966607879092 70-71 0.5409503147665822 72-73 0.5266427428491339 74-75 0.5309861128955022 76-77 0.5060208598558731 78-79 0.48017963302537986 80-81 0.4884648851306369 82-83 0.5015314941776942 84-85 0.4953631787336923 86-87 0.49514418528597626 88-89 0.505473376236583 90-91 0.5243433116481155 92-93 0.5306941216318808 94-95 0.5266792417570866 96-97 0.5274457188240927 98-99 0.5278107079036195 100-101 0.5026994592321797 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1369904.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.890032325621554 #Duplication Level Percentage of deduplicated Percentage of total 1 68.79621611075815 26.066908523170724 2 14.338991574905746 10.866097085799877 3 6.104055406794548 6.938485700424914 4 3.1748141050821697 4.811752362776107 5 1.9052171684188772 3.609437004936021 6 1.2217101303331128 2.7774381798516585 7 0.8096735716210033 2.147499046134489 8 0.6005621709010295 1.820425605518837 9 0.412537327445894 1.4067947405205403 >10 2.3384513665983775 16.342378991843248 >50 0.1910712955006345 4.946468649366004 >100 0.09355560241299275 5.914216904169602 >500 0.00502571176346729 1.3485708194664203 >1k 0.006572084613764918 5.984891820100907 >5k 0.0013530762440104244 3.7200867665865363 >10k+ 1.9329660628720347E-4 1.298547799334146 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17730 1.294251276001822 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9850 0.7190284866676789 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8490 0.6197514570364054 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7815 0.5704779313002956 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7036 0.5136126327100293 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6442 0.4702519300622526 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6138 0.44806059402702675 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5022 0.3665950314766582 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4871 0.3555723612749507 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4401 0.32126338779943703 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4276 0.3121386608112685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4275 0.31206566299536315 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4105 0.29965603429145404 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4097 0.2990720517642112 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3900 0.28469148203085765 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3732 0.27242784895875916 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3407 0.24870355878952102 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2871 0.20957672946425443 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2560 0.18687440871769115 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2460 0.17957462712715636 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2435 0.17774968172952266 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2424 0.1769467057545638 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2388 0.1743187843819713 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2303 0.16811397003001669 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2232 0.16293112510073698 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2189 0.159792219016807 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2110 0.15402539156028452 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1850 0.135045959424894 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1735 0.12665121059577897 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1717 0.1253372499094827 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1547 0.11292762120557352 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1529 0.11161366051927726 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1449 0.10577383524684941 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1412 0.10307291605835155 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1374 0.1002989990539483 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.649890795267406E-5 0.0 12-13 0.0 0.0 0.0 7.299781590534812E-5 0.0 14-15 0.0 0.0 0.0 1.0949672385802218E-4 0.0 16-17 0.0 0.0 0.0 2.554923556687184E-4 0.0 18-19 0.0 0.0 0.0 4.7448580338476276E-4 0.0 20-21 0.0 0.0 0.0 9.854705147221996E-4 0.0 22-23 0.0 0.0 0.0 0.0018614443055863768 0.0 24-25 0.0 0.0 0.0 0.003321400623693339 0.0 26-27 0.0 0.0 0.0 0.005036849297469019 0.0 28-29 0.0 0.0 0.0 0.011497156005092327 0.0 30-31 0.0 0.0 0.0 0.03022109578481412 0.0 32-33 0.0 0.0 0.0 0.06533304523528656 0.0 34-35 0.0 0.0 0.0 0.12132237003468857 0.0 36-37 0.0 0.0 0.0 0.20552535068150762 0.0 38-39 0.0 0.0 0.0 0.32078890199605226 0.0 40-41 0.0 0.0 0.0 0.4551048832618928 0.0 42-43 0.0 0.0 0.0 0.5933262476786694 0.0 44-45 0.0 0.0 0.0 0.7550529088169682 0.0 46-47 0.0 0.0 0.0 0.9606512573143811 0.0 48-49 0.0 0.0 0.0 1.163366192083533 0.0 50-51 0.0 0.0 0.0 1.3918493558672724 0.0 52-53 0.0 0.0 0.0 1.6094558450811152 0.0 54-55 0.0 0.0 0.0 1.8554584846821383 0.0 56-57 0.0 0.0 0.0 2.160662352982399 0.0 58-59 0.0 0.0 0.0 2.4884590453053645 0.0 60-61 0.0 0.0 0.0 2.861514383489646 0.0 62-63 0.0 0.0 0.0 3.225116504514185 0.0 64-65 0.0 0.0 0.0 3.5927700043214705 0.0 66-67 0.0 0.0 0.0 4.026924514418528 0.0 68-69 0.0 0.0 0.0 4.517287342762705 0.0 70-71 0.0 0.0 0.0 5.0434191009005005 0.0 72-73 0.0 0.0 0.0 5.576631647181117 0.0 74-75 0.0 0.0 0.0 6.140466777234025 0.0 76-77 0.0 0.0 0.0 6.6995935481610385 0.0 78-79 0.0 7.299781590534812E-5 0.0 7.395773718450344 0.0 80-81 0.0 7.299781590534812E-5 0.0 8.151556605426366 0.0 82-83 0.0 1.4599563181069624E-4 0.0 8.872264041859868 0.0 84-85 0.0 1.4599563181069624E-4 0.0 9.631587322907299 0.0 86-87 0.0 1.4599563181069624E-4 0.0 10.437848199581868 0.0 88-89 0.0 1.4599563181069624E-4 0.0 11.279586014786437 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 260 0.0 27.32775 5 GTACATG 10225 0.0 25.357817 1 GTGTACG 150 1.0627227E-6 25.326855 1 TACATGG 10260 0.0 24.930582 2 GTAAGCG 135 1.8189894E-12 24.660378 94-95 GAGTACT 6340 0.0 23.86342 12-13 ACATGGG 10325 0.0 23.811085 3 CATGGGG 2435 0.0 23.344477 4 ATGGGAT 2360 0.0 22.279064 5 GTAAGGT 215 4.553476E-8 22.032482 4 CATGGGA 7355 0.0 21.962084 4 GTACTTT 6970 0.0 21.742424 14-15 AGTACTT 6635 0.0 21.160934 12-13 ATGGGAG 2205 0.0 20.837667 5 CTTTGCG 325 2.3646862E-11 20.40472 2 CCCTATA 305 2.1645974E-10 20.189682 2 TAGGACA 585 0.0 19.433777 4 GTGTAGG 440 0.0 19.426851 1 GACAATG 550 0.0 18.939627 7 ACTTTTT 8130 0.0 18.874565 16-17 >>END_MODULE