##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939124_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1369904 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.426143729779604 34.0 31.0 34.0 31.0 34.0 2 32.619768976512226 34.0 31.0 34.0 31.0 34.0 3 32.72797509898504 34.0 31.0 34.0 31.0 34.0 4 36.186465620948624 37.0 35.0 37.0 35.0 37.0 5 36.15828116422757 37.0 35.0 37.0 35.0 37.0 6 36.223071835690675 37.0 37.0 37.0 35.0 37.0 7 36.17641820156741 37.0 37.0 37.0 35.0 37.0 8 36.16585322767143 37.0 37.0 37.0 35.0 37.0 9 37.87404664852428 39.0 38.0 39.0 35.0 39.0 10-11 37.78495135425548 39.0 38.0 39.0 35.0 39.0 12-13 37.72070342155363 39.0 37.5 39.0 35.0 39.0 14-15 39.02733220722036 40.0 38.0 41.0 36.0 41.0 16-17 39.15542512468027 40.5 38.5 41.0 36.0 41.0 18-19 39.245014249173664 41.0 39.0 41.0 36.0 41.0 20-21 39.22268932713533 41.0 39.0 41.0 36.0 41.0 22-23 39.152651207675866 41.0 39.0 41.0 35.5 41.0 24-25 39.05766681460891 40.5 39.0 41.0 35.0 41.0 26-27 38.873398427919035 40.0 38.0 41.0 35.0 41.0 28-29 38.72254296651444 40.0 38.0 41.0 35.0 41.0 30-31 38.53863701398054 40.0 38.0 41.0 34.0 41.0 32-33 38.39806402492437 40.0 38.0 41.0 34.0 41.0 34-35 38.24984998948831 40.0 38.0 41.0 34.0 41.0 36-37 38.06484578481412 40.0 37.5 41.0 33.5 41.0 38-39 37.90509261962882 40.0 37.0 41.0 33.0 41.0 40-41 37.73181989394877 40.0 37.0 41.0 33.0 41.0 42-43 37.51108836823602 40.0 37.0 41.0 33.0 41.0 44-45 37.18917347492963 40.0 37.0 41.0 32.5 41.0 46-47 36.78063280346652 40.0 36.0 41.0 31.0 41.0 48-49 36.358804339574164 40.0 36.0 41.0 30.0 41.0 50-51 36.07369239012369 39.0 35.0 41.0 30.0 41.0 52-53 35.81305405342272 39.0 35.0 41.0 29.0 41.0 54-55 35.59668633714479 39.0 35.0 40.0 28.0 41.0 56-57 35.36693702624417 38.5 35.0 40.0 28.0 41.0 58-59 35.05201313376703 38.0 35.0 40.0 27.0 41.0 60-61 34.63554197958397 38.0 34.0 40.0 26.0 41.0 62-63 34.2620942781392 37.0 34.0 40.0 26.0 41.0 64-65 33.804243217042945 36.5 33.5 39.5 25.0 41.0 66-67 33.360766520865695 36.0 33.0 39.0 23.5 40.0 68-69 32.862579786612784 35.5 33.0 39.0 22.5 40.0 70-71 32.32584254079118 35.0 32.0 37.5 21.5 40.0 72-73 31.74385321891169 35.0 31.5 37.0 19.0 39.0 74-75 31.22952374764947 35.0 31.0 36.5 18.0 39.0 76-77 29.23893937093402 32.5 28.5 34.5 11.5 36.5 78-79 30.210352696247327 34.0 30.0 35.0 11.0 37.0 80-81 30.224145268573565 34.0 30.0 35.0 8.5 37.0 82-83 29.859871202653615 34.0 30.0 35.0 4.5 36.5 84-85 29.416343043016155 34.0 29.5 35.0 2.0 36.0 86-87 28.77621351569161 34.0 29.0 35.0 2.0 35.5 88-89 28.09484679218398 33.0 28.0 35.0 2.0 35.0 90-91 27.52504190074633 33.0 26.5 35.0 2.0 35.0 92-93 27.11879153575725 33.0 26.0 35.0 2.0 35.0 94-95 26.605298984454386 32.5 25.0 34.0 2.0 35.0 96-97 25.8031227005688 32.0 24.5 34.0 2.0 35.0 98-99 24.661082090423854 32.0 11.0 34.0 2.0 35.0 100-101 18.941019589693877 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1915817102278794 1101 2 0.20656909091415798 1101 3 0.17934534895209708 1101 4 0.218099355351157 1101 5 0.16861205244638455 1101 6 0.15937078665297122 1101 7 0.18335000790537492 1101 8 0.1993968796441905 1101 9 0.3502386964909263 1101 10-11 0.2602640364642781 1101 12-13 0.3005793575557121 1101 14-15 0.3240269186754361 1101 16-17 0.34660549447990263 1101 18-19 0.3625241974043405 1101 20-21 0.32870271640376814 1101 22-23 0.26278331905713515 1101 24-25 0.2827984227252145 1101 26-27 0.1983091908490806 1101 28-29 0.17230011242962462 1101 30-31 0.20210063934534617 1101 32-33 0.2194288889233249 1101 34-35 0.01813711939420415 1101 36-37 0.06337454076009408 1101 38-39 -0.1080626770450337 1101 40-41 0.016687885294238924 1101 42-43 0.0675830156583217 1101 44-45 -0.08111498562411157 1101 46-47 -0.09902876045034503 1101 48-49 -0.028011046944513396 1101 50-51 -0.12124382715308712 1101 52-53 -0.05627684185309789 1101 54-55 -0.03688749825964521 1101 56-57 -0.09832524693037215 1101 58-59 -0.04530463277306751 1101 60-61 -0.09180001258956594 1101 62-63 -0.05019125824473747 1101 64-65 -0.16303097020509227 1101 66-67 -0.17074741695772389 1101 68-69 0.05673996861919761 1101 70-71 0.05578716444822618 1101 72-73 0.13433180447821158 1101 74-75 -0.052678808851048586 1101 76-77 0.11246537298278625 1101 78-79 0.35945233491341 1101 80-81 0.4853050627785329 1101 82-83 0.32700007114872776 1101 84-85 0.3253847813170978 1101 86-87 0.5708463518287914 1101 88-89 0.589449229768384 1101 90-91 0.9002877347741958 1101 92-93 0.8480675887867939 1101 94-95 0.5888233066127952 1101 96-97 0.30466390166445834 1101 98-99 0.07796710612361935 1101 100-101 -0.048452041551282576 1102 1 0.005201483527137896 1102 2 0.031776732910451244 1102 3 0.030250880033840133 1102 4 0.08659828197211539 1102 5 0.09710303953114874 1102 6 0.06555784366732098 1102 7 0.093190793225709 1102 8 0.08819712322608098 1102 9 0.1271647084927352 1102 10-11 0.10796176367568933 1102 12-13 0.14097001356952177 1102 14-15 0.2171707823004212 1102 16-17 0.22172391304762584 1102 18-19 0.2456145269721759 1102 20-21 0.16975243100442583 1102 22-23 0.18220743289312935 1102 24-25 0.16469512396354702 1102 26-27 0.08212988066373583 1102 28-29 0.1426621321551238 1102 30-31 0.13149773563341682 1102 32-33 0.12037122707819492 1102 34-35 0.10080619104658695 1102 36-37 0.20701578164022294 1102 38-39 0.11353825719941568 1102 40-41 0.07098159417534333 1102 42-43 0.2036215725127235 1102 44-45 0.14697786840789462 1102 46-47 0.08963504644163578 1102 48-49 0.019603430761193863 1102 50-51 -0.050063572702512715 1102 52-53 -0.10048232938301993 1102 54-55 -0.11113989071939301 1102 56-57 0.011320518714555305 1102 58-59 -0.08555923751135452 1102 60-61 -0.06358536761401723 1102 62-63 -0.19381669713536098 1102 64-65 0.05036718982665889 1102 66-67 -0.04994930086320437 1102 68-69 -0.08571303973273814 1102 70-71 -0.08240508795193335 1102 72-73 -0.20804874597060063 1102 74-75 -0.28038586309041236 1102 76-77 -0.1584697579232106 1102 78-79 -0.17031321752029172 1102 80-81 -0.16919484279494412 1102 82-83 -0.027285929444808232 1102 84-85 -0.07606244967330156 1102 86-87 0.040841842480563884 1102 88-89 -0.21761117622712334 1102 90-91 0.17850295787130932 1102 92-93 0.11197623016257197 1102 94-95 -0.055988052319762005 1102 96-97 0.2886191010971295 1102 98-99 0.0026937509758333533 1102 100-101 -0.40259775131113074 1103 1 0.17371225329721796 1103 2 0.19851841142304494 1103 3 0.1327787049725302 1103 4 0.1639666881709161 1103 5 0.16122702742824657 1103 6 0.09880071717061156 1103 7 0.08198803397808518 1103 8 0.1419523944793113 1103 9 0.2274815407053481 1103 10-11 0.20243249940078556 1103 12-13 0.1182109738994015 1103 14-15 0.09834013528239183 1103 16-17 0.2453422468339994 1103 18-19 0.3077180225241918 1103 20-21 0.27806099557476927 1103 22-23 0.22514280238959827 1103 24-25 0.28357738804386656 1103 26-27 0.26896953504479626 1103 28-29 0.27956060770646474 1103 30-31 0.40641280885537867 1103 32-33 0.3861749604400444 1103 34-35 0.34751978096500125 1103 36-37 0.23987319823224595 1103 38-39 0.33075993725680775 1103 40-41 0.38584975899939167 1103 42-43 0.36009776850605846 1103 44-45 0.34593676340170987 1103 46-47 0.3924968597675971 1103 48-49 0.5381714620493057 1103 50-51 0.6977224638052277 1103 52-53 0.7301561476834095 1103 54-55 0.6208554095285095 1103 56-57 0.6657855060071043 1103 58-59 0.5822448612547362 1103 60-61 0.6246390351505724 1103 62-63 0.5044514947226588 1103 64-65 0.5351657793068796 1103 66-67 0.4779268835441286 1103 68-69 0.6390380471963368 1103 70-71 0.5387550110934072 1103 72-73 0.358476363687803 1103 74-75 0.5012182615213447 1103 76-77 0.5737672884222285 1103 78-79 0.5177771294206366 1103 80-81 0.5971454852073492 1103 82-83 0.2888206735286296 1103 84-85 0.2403646247013178 1103 86-87 0.12754291061954248 1103 88-89 0.5881003445526432 1103 90-91 0.7152283120237719 1103 92-93 0.8027413806987198 1103 94-95 0.6589810442850954 1103 96-97 0.36137167935082104 1103 98-99 0.5107864893835306 1103 100-101 0.04207135732533018 1104 1 0.16822110607431995 1104 2 0.14831502276707198 1104 3 0.19696289817442647 1104 4 0.11100594529991525 1104 5 0.07329244442696137 1104 6 0.0905404351290997 1104 7 0.16838282279344696 1104 8 0.1480582120185545 1104 9 0.17456306941190292 1104 10-11 0.2394135464885494 1104 12-13 0.28149637634560776 1104 14-15 0.13318092388986003 1104 16-17 0.21950669020048252 1104 18-19 0.42155503440874753 1104 20-21 0.3649051797679732 1104 22-23 0.2456566445988031 1104 24-25 0.34389165296885693 1104 26-27 0.26082290117901863 1104 28-29 0.2429267419360457 1104 30-31 0.37578220007536345 1104 32-33 0.3567695344326296 1104 34-35 0.5183582827494178 1104 36-37 0.4798413213049031 1104 38-39 0.515211619457709 1104 40-41 0.5252790927023128 1104 42-43 0.6415918262310143 1104 44-45 0.7335699015466091 1104 46-47 0.8688126492412849 1104 48-49 0.8114890879111911 1104 50-51 0.8174615580847089 1104 52-53 0.7033743313915366 1104 54-55 0.8530774392572127 1104 56-57 0.664048213466998 1104 58-59 0.7030636132037316 1104 60-61 0.7413061638016956 1104 62-63 0.6034057929105217 1104 64-65 0.6015882871894931 1104 66-67 0.5470589302507562 1104 68-69 0.7644806860515416 1104 70-71 0.6485588986925919 1104 72-73 0.5558943325731498 1104 74-75 0.5425023473118564 1104 76-77 0.39714936111548127 1104 78-79 0.6170051533976313 1104 80-81 0.8224065641720415 1104 82-83 0.8593061036939069 1104 84-85 0.7898871637678759 1104 86-87 0.8560949358438954 1104 88-89 0.9129896415278012 1104 90-91 1.0337571225292983 1104 92-93 0.9811575824935304 1104 94-95 0.8821146848380934 1104 96-97 0.4886915920357602 1104 98-99 0.29915383632230785 1104 100-101 -0.504061139816141 1105 1 0.0049214351336672735 1105 2 0.07455256146801759 1105 3 0.07217127540936019 1105 4 0.036019318401336875 1105 5 -0.09539861261539073 1105 6 -0.0241375567984079 1105 7 0.10765046312995707 1105 8 0.047633190540537385 1105 9 0.11863400358382847 1105 10-11 0.10735441306552218 1105 12-13 0.02439274696693161 1105 14-15 0.02329876928059349 1105 16-17 0.13197917403118709 1105 18-19 0.10479570160493523 1105 20-21 0.028827631989528868 1105 22-23 0.12714178829886436 1105 24-25 0.1431025589356878 1105 26-27 0.24613226559198864 1105 28-29 0.15734278317383144 1105 30-31 0.28424015990743356 1105 32-33 0.2929355878277775 1105 34-35 0.354599873905336 1105 36-37 0.321174416894209 1105 38-39 0.2771580946323624 1105 40-41 0.4504801755182797 1105 42-43 0.34429238222783454 1105 44-45 0.27314001712262836 1105 46-47 0.3868428319259536 1105 48-49 0.5798617840905393 1105 50-51 0.4131604444700798 1105 52-53 0.5297704021311844 1105 54-55 0.5107753206337478 1105 56-57 0.5553993019564061 1105 58-59 0.39955580817544956 1105 60-61 0.319133075344709 1105 62-63 0.3771001729659673 1105 64-65 0.307515894133779 1105 66-67 0.33014203799743314 1105 68-69 0.3369077823865041 1105 70-71 0.4838784752920944 1105 72-73 0.28859310548978456 1105 74-75 0.15905905736680737 1105 76-77 -0.02595579198626652 1105 78-79 -0.1122247180584992 1105 80-81 0.2299853033722492 1105 82-83 0.35423761720479163 1105 84-85 0.3770211055097832 1105 86-87 0.46435319069468406 1105 88-89 0.587435184637819 1105 90-91 0.4479446053063647 1105 92-93 0.5708932003308185 1105 94-95 0.35744885027551376 1105 96-97 0.4104957449916249 1105 98-99 -0.09783146137103671 1105 100-101 -1.1737280280170133 1106 1 0.013005669170475187 1106 2 0.059569114580313 1106 3 0.10895813679174893 1106 4 0.05814520008087243 1106 5 0.07388385827569266 1106 6 0.017550318130652443 1106 7 0.13569718368745498 1106 8 0.1256227021277212 1106 9 0.1373108884167138 1106 10-11 0.14114549943857213 1106 12-13 0.18653247827678143 1106 14-15 0.1134606551223385 1106 16-17 -0.025253752733412682 1106 18-19 -0.04859078293584673 1106 20-21 0.09824707523682719 1106 22-23 0.11960373686626014 1106 24-25 0.2825424901264242 1106 26-27 0.19090168326745527 1106 28-29 0.157027326145176 1106 30-31 0.13135505392911284 1106 32-33 0.25612800076621056 1106 34-35 0.19373629581188823 1106 36-37 0.26584630196178693 1106 38-39 0.40389988985412373 1106 40-41 0.32498557851996424 1106 42-43 0.23361024526401053 1106 44-45 0.4525863001244588 1106 46-47 0.46236598769101533 1106 48-49 0.3034473631629666 1106 50-51 0.4896845286236484 1106 52-53 0.4106730298425134 1106 54-55 0.35775549774077575 1106 56-57 0.2699228825978892 1106 58-59 0.43456391862137167 1106 60-61 0.3400772885217336 1106 62-63 0.11907857315078729 1106 64-65 0.23466360787801221 1106 66-67 0.3269633893482933 1106 68-69 0.2514448347194502 1106 70-71 -0.009852616335528808 1106 72-73 0.04902071282594633 1106 74-75 0.19854726719846028 1106 76-77 0.3102735486262489 1106 78-79 0.359097342537293 1106 80-81 0.5317661268766329 1106 82-83 0.5833877363469675 1106 84-85 0.4535982230188971 1106 86-87 0.364631280542941 1106 88-89 0.31671636322185037 1106 90-91 0.5472562689261515 1106 92-93 0.2019425841380844 1106 94-95 0.23663063703631693 1106 96-97 -0.02198486887861506 1106 98-99 0.07467751010494439 1106 100-101 -0.30175938718876694 1107 1 0.06592887359197164 1107 2 0.04404250006977861 1107 3 -7.588369074369439E-4 1107 4 -0.036941598736710546 1107 5 -0.06905565831223015 1107 6 0.03873629141838819 1107 7 0.16078757176527603 1107 8 0.064203164296039 1107 9 0.10354266124863898 1107 10-11 0.029401906851703075 1107 12-13 0.1361281758334698 1107 14-15 0.1825267762992624 1107 16-17 0.16721988954866163 1107 18-19 0.13524324733649706 1107 20-21 0.15508753407851827 1107 22-23 0.11224312710054818 1107 24-25 0.13996831937686238 1107 26-27 0.07257427853605236 1107 28-29 0.15027368071155678 1107 30-31 0.16219176009708747 1107 32-33 0.11295328549960004 1107 34-35 0.09041229066436784 1107 36-37 0.1153468695318054 1107 38-39 0.14594646853041837 1107 40-41 0.1889680816058572 1107 42-43 0.17961463991922244 1107 44-45 0.516967123553286 1107 46-47 0.551702383081583 1107 48-49 0.5132629685661101 1107 50-51 0.5546711491454701 1107 52-53 0.5263432147623703 1107 54-55 0.5075338682166262 1107 56-57 0.5471614283346895 1107 58-59 0.6189555973738265 1107 60-61 0.5858502780253332 1107 62-63 0.7317956431471941 1107 64-65 0.6497577603019309 1107 66-67 0.7951275265105551 1107 68-69 0.5346599364589295 1107 70-71 0.6396584563863925 1107 72-73 0.42279430929387374 1107 74-75 0.5360617482585468 1107 76-77 0.5246142482110052 1107 78-79 0.8925355328497915 1107 80-81 0.8878896091010411 1107 82-83 0.6240721469933561 1107 84-85 0.41818389690979885 1107 86-87 0.5889901015147281 1107 88-89 0.5546005672669168 1107 90-91 0.7459151112000519 1107 92-93 0.82112499151037 1107 94-95 0.7789859504546044 1107 96-97 0.47794743125357897 1107 98-99 0.7931058810185121 1107 100-101 -0.19540435390786115 1108 1 0.03866181290847237 1108 2 0.0503613137126635 1108 3 0.0475550694691762 1108 4 0.033646536558769924 1108 5 0.09278269656358162 1108 6 0.08073478410565116 1108 7 0.019437903544073265 1108 8 0.06135910316487525 1108 9 0.09907172235146788 1108 10-11 0.06462599212254361 1108 12-13 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30.0 6 71.0 7 184.0 8 370.0 9 682.0 10 1277.0 11 1964.0 12 3355.0 13 4656.0 14 5561.0 15 7706.0 16 10642.0 17 16113.0 18 15194.0 19 8826.0 20 7669.0 21 8289.0 22 8605.0 23 9435.0 24 10779.0 25 12523.0 26 14663.0 27 17405.0 28 21177.0 29 25749.0 30 31425.0 31 38770.0 32 48681.0 33 64030.0 34 90500.0 35 137127.0 36 219077.0 37 324088.0 38 195385.0 39 7882.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.95833214219482 19.746144559502316 14.610688287479997 20.68483501082287 2 15.375529964143473 22.70648162206987 38.64913161798199 23.26885679580467 3 21.449824221259302 26.364986159614105 30.13430138170266 22.050888237423933 4 12.601174972844811 16.927609525922986 38.28144161926675 32.18977388196545 5 12.669792919795839 39.431303215407794 33.39489482474684 14.504009040049523 6 29.2516847895911 37.261151146357705 17.272816197339374 16.21434786671183 7 27.168692112731986 34.68578820121702 20.783792148938904 17.36172753711209 8 25.505217883880917 32.72565084852661 19.971399455728285 21.79773181186419 9 25.96692906948224 18.716931989394876 21.799337763814105 33.51680117730878 10-11 24.969194921687944 26.47623483105386 26.597082715285158 21.957487531973044 12-13 28.06944866209603 24.480109555122112 26.782387670960883 20.668054111820975 14-15 23.659816635782338 25.845675202469675 27.904370877653896 22.590137284094087 16-17 20.79564716548918 29.25705871961199 28.206408510029448 21.74088560486938 18-19 20.45701742603861 28.866183323794953 30.395597063735853 20.281202186430583 20-21 22.499386818346395 26.285126549013654 29.339464663217274 21.876021969422673 22-23 21.482563741692847 26.558685864118946 29.652150807647836 22.306599586540372 24-25 22.64842012594318 26.70308529031424 29.7148667771124 20.933627806630177 26-27 22.235788159674126 25.539574516234413 31.58774393593471 20.636893388156754 28-29 20.61845209591329 27.93254125836555 30.810589647157755 20.638416998563404 30-31 21.074469451874002 26.886300061902148 30.13879074738084 21.900439738843012 32-33 21.278060360434015 27.141828916478815 30.178757051589017 21.40135367149815 34-35 22.566631404422893 26.263545483549034 29.82576217186219 21.344060940165885 36-37 22.390426785257272 25.66910752926461 30.954754899401582 20.985710786076535 38-39 21.837564808263917 26.62189502612774 30.740382165233054 20.800158000375287 40-41 21.268918633313877 27.16672488767361 30.538067960523602 21.02628851848891 42-43 20.892921999821137 27.550231881571573 29.040442847261133 22.516403271346157 44-45 21.63750118169398 26.688946388870942 28.923269644134 22.750282785301078 46-47 22.522131533196113 27.481974841247563 28.541127438867036 21.454766186689287 48-49 22.49368093513035 27.006360127385047 28.654518245444343 21.84544069204026 50-51 21.126869032836872 27.686359445591734 29.637278239979324 21.549493281592067 52-53 21.786563146030673 28.558205538490288 27.91082440813371 21.744406907345333 54-55 20.665718181712002 27.234426645954752 28.709274518506405 23.390580653826838 56-57 22.092788983753607 27.363523283383362 27.901882175685305 22.64180555717773 58-59 22.992151505186037 26.67882344481873 27.932071447107976 22.39695360288726 60-61 21.781871763603586 27.179287646952975 29.55955261399178 21.47928797545166 62-63 21.645487288723295 27.138024623946812 29.087018919822526 22.129469167507366 64-65 20.986750831015883 28.39838684186225 28.611328388057316 22.003533939064553 66-67 21.436888171870326 27.302043259579378 27.8355610386387 23.425507529911595 68-69 20.7450683805483 27.70620381739685 28.413917306768553 23.13481049528629 70-71 22.02069929089818 27.622377367140132 27.72253051678149 22.634392825180193 72-73 21.7139668181128 26.572701444772772 28.748583842371435 22.96474789474299 74-75 20.699224179212557 28.91104048166879 27.63007480816174 22.759660530956914 76-77 21.325780229070073 27.11964346581101 27.94335610200198 23.611220203116932 78-79 21.325317565599317 27.233180284686544 28.38681275037106 23.054689399343076 80-81 21.6634298088508 27.73614249522866 27.882540202796047 22.717887493124493 82-83 21.21999059788131 27.279977283079692 28.378010429927937 23.12202168911106 84-85 20.951176140809867 27.346441794461512 27.988421086441097 23.71396097828753 86-87 21.076986708387622 27.556145352027865 27.83591300274689 23.53095493683762 88-89 20.988635297429163 27.205356305122912 27.4333980343404 24.372610363107526 90-91 21.10306336445981 26.53961717290259 27.945879657151824 24.411439805485777 92-93 21.43781608054287 27.399766698980365 27.90947394853946 23.252943271937305 94-95 20.936759072168563 26.925207897779696 27.957980997208566 24.18005203284318 96-97 19.939462911269697 26.893782338032445 28.629487905721863 24.537266844976 98-99 20.774055935428912 27.336500958828413 27.08841733818721 24.801025767555466 100-101 20.923206073436017 25.938535659537195 28.038360464267463 25.099897802759326 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1705.0 1 1115.5 2 679.0 3 833.0 4 681.0 5 665.0 6 1164.5 7 2260.5 8 3433.0 9 3732.0 10 3064.5 11 2450.0 12 2415.5 13 2493.5 14 2593.5 15 2693.0 16 2732.0 17 2824.5 18 2989.5 19 3171.5 20 3320.0 21 3627.5 22 4115.0 23 4722.0 24 5571.5 25 6480.0 26 7998.5 27 9799.5 28 10911.0 29 12256.0 30 14383.5 31 16755.0 32 19663.0 33 22708.0 34 25916.5 35 28796.0 36 31915.5 37 35307.5 38 36523.0 39 36120.5 40 35524.0 41 36366.5 42 37787.5 43 39318.0 44 40804.0 45 42713.5 46 46097.5 47 61649.5 48 99986.5 49 98800.5 50 63006.5 51 47111.0 52 39467.0 53 37609.0 54 36555.0 55 33660.5 56 29945.5 57 26313.5 58 23618.5 59 21145.0 60 18494.5 61 15921.5 62 13425.0 63 10779.5 64 8198.0 65 6231.5 66 4562.5 67 3879.0 68 3080.5 69 1965.5 70 1414.5 71 1090.0 72 947.5 73 835.5 74 645.5 75 483.0 76 309.5 77 176.0 78 109.5 79 67.5 80 35.5 81 15.0 82 10.5 83 6.5 84 1.5 85 1.5 86 1.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4133866314719864 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.474836192901108E-4 16-17 0.002773917004403228 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.649890795267405E-5 26-27 0.003759387519125428 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012190635256193134 36-37 0.02733768205655287 38-39 0.020950373164834907 40-41 0.009015230264310491 42-43 0.009599212791553277 44-45 0.0035403940714093833 46-47 0.006022319812191219 48-49 0.0030294093600719465 50-51 8.029759749588292E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0017154486737756805 60-61 0.002591422464639858 62-63 0.001313960686296266 64-65 0.0032484028077879906 66-67 0.002481925740781836 68-69 0.005109847113374368 70-71 1.459956318106962E-4 72-73 0.0 74-75 0.0 76-77 0.0936926967145143 78-79 0.013650591574300097 80-81 4.014879874794146E-4 82-83 0.0 84-85 0.0 86-87 0.10562783961503872 88-89 0.4146275943423773 90-91 0.414992583421904 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.1899344771604434E-4 100-101 2.919912636213924E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1369904.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.198196116365914 #Duplication Level Percentage of deduplicated Percentage of total 1 67.16970674626516 24.31422217880093 2 14.87870876562622 10.77164835712861 3 6.394466610296109 6.9440446925715635 4 3.3647643188468 4.871935947958674 5 2.0575177873977424 3.7239216190567372 6 1.307106451681824 2.83889374097675 7 0.8960264743608753 2.2704179431059615 8 0.6107642379027984 1.7686850931574614 9 0.48918108687650597 1.5936725627255515 >10 2.49921801591017 16.607284999653967 >50 0.2174843990974402 5.413738926833539 >100 0.1017329394199466 6.372708523638633 >500 0.004844424115772997 1.2983172629839665 >1k 0.00666108315918787 5.766402070723994 >5k 0.001614808038590999 4.033485726180647 >10k+ 2.0185100482387488E-4 1.4106203545032618 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 19306 1.4092958338686508 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9208 0.6721638888564454 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8561 0.6249343019656852 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7319 0.5342710146112428 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6757 0.4932462420724372 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6639 0.48463249979560613 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6280 0.4584262838855861 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5239 0.38243555752811875 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5200 0.3795886427078102 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4632 0.33812588327357246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4570 0.3336000186874409 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4552 0.3322860580011446 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4549 0.33206706455342855 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4479 0.3269572174400542 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3869 0.28242854973779186 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3603 0.26301113070696924 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3320 0.24235274880575575 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3082 0.2249792686202829 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2494 0.18205655286793818 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2460 0.17957462712715636 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2445 0.17847965988857611 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2432 0.1775306882818066 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2337 0.17059589577079853 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2202 0.16074119062357656 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2160 0.15767528235555192 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2131 0.15555834569429683 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2063 0.15059449421273316 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1924 0.14044779780188976 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1705 0.12446127611861853 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1654 0.12073838750744578 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1650 0.12044639624382439 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1633 0.11920543337343348 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1612 0.11767247923942115 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1547 0.11292762120557352 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.649890795267406E-5 0.0 12-13 0.0 0.0 0.0 7.299781590534812E-5 0.0 14-15 0.0 0.0 0.0 1.0949672385802218E-4 0.0 16-17 0.0 0.0 0.0 2.554923556687184E-4 0.0 18-19 0.0 0.0 0.0 4.7448580338476276E-4 0.0 20-21 0.0 0.0 0.0 9.854705147221996E-4 0.0 22-23 0.0 0.0 0.0 0.0017884464896810288 0.0 24-25 0.0 0.0 0.0 0.003248402807787991 0.0 26-27 0.0 0.0 0.0 0.005000350389516345 0.0 28-29 0.0 0.0 0.0 0.011716149452808373 0.0 30-31 0.0 0.0 0.0 0.03087807612796225 0.0 32-33 0.0 0.0 0.0 0.06704849390906224 0.0 34-35 0.0 0.0 0.0 0.12332980997208562 0.0 36-37 0.0 0.0 0.0 0.20829926768591084 0.0 38-39 0.0 0.0 0.0 0.3261177425571427 0.0 40-41 0.0 0.0 0.0 0.4623316670365223 0.0 42-43 0.0 0.0 0.0 0.6020494866793585 0.0 44-45 0.0 0.0 0.0 0.7657470888471016 0.0 46-47 0.0 0.0 0.0 0.9734623740057697 0.0 48-49 0.0 0.0 0.0 1.1775277683691705 0.0 50-51 0.0 0.0 0.0 1.4082008666300703 0.0 52-53 0.0 0.0 0.0 1.627960791413121 0.0 54-55 0.0 0.0 0.0 1.8756423807799671 0.0 56-57 0.0 0.0 0.0 2.183948656256205 0.0 58-59 0.0 0.0 0.0 2.515176245926722 0.0 60-61 0.0 0.0 0.0 2.8916989803665074 0.0 62-63 0.0 0.0 0.0 3.258586003106787 0.0 64-65 0.0 0.0 0.0 3.6296704002616242 0.0 66-67 0.0 0.0 0.0 4.069007755287961 0.0 68-69 0.0 0.0 0.0 4.56458992746937 0.0 70-71 0.0 0.0 0.0 5.096561510879594 0.0 72-73 0.0 0.0 0.0 5.6340809282986255 0.0 74-75 0.0 0.0 0.0 6.2041938705193935 0.0 76-77 0.0 0.0 0.0 6.7676275125848235 0.0 78-79 0.0 0.0 0.0 7.465778623903573 0.0 80-81 0.0 0.0 0.0 8.224116434436283 0.0 82-83 0.0 0.0 0.0 8.949386234363867 0.0 84-85 0.0 0.0 0.0 9.712870390917903 0.0 86-87 0.0 0.0 0.0 10.523511136546794 0.0 88-89 0.0 0.0 0.0 11.367730877492146 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 330 0.0 35.94709 94-95 CGTCAAG 80 4.4127143E-4 29.780264 1 GTCGTCC 95 0.0012342046 24.97377 8 GAGTACT 6760 0.0 24.288391 12-13 GTACATG 9565 0.0 23.911386 1 CGTATAG 145 2.2791259E-5 23.002686 1 TACATGG 9725 0.0 22.88342 2 GTACTTT 7320 0.0 22.20337 14-15 ACATGGG 9850 0.0 21.87043 3 ATGGGAG 2195 0.0 21.40121 5 AGTACTT 7045 0.0 21.318768 12-13 GTGTAGG 585 0.0 20.362574 1 CATGGGA 6765 0.0 20.130371 4 ACGTCAG 120 0.0047929753 19.7709 9 ATGGGAT 2350 0.0 19.58581 5 ACTTTTT 8520 0.0 19.214674 16-17 ATGGGTA 445 0.0 19.193323 5 CATGGGG 2585 0.0 18.906641 4 CATGGGT 950 0.0 18.480589 4 CGCTACG 65 0.007594063 18.257393 30-31 >>END_MODULE