##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939123_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 987816 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21065461583939 33.0 31.0 34.0 31.0 34.0 2 32.22762032605262 34.0 31.0 34.0 30.0 34.0 3 32.25611348672222 34.0 31.0 34.0 30.0 34.0 4 35.71596025980547 37.0 35.0 37.0 35.0 37.0 5 35.80209573442827 37.0 35.0 37.0 35.0 37.0 6 35.95128141273273 37.0 37.0 37.0 35.0 37.0 7 35.90912173927128 37.0 36.0 37.0 35.0 37.0 8 35.914414222891715 37.0 37.0 37.0 35.0 37.0 9 37.55550223928343 39.0 38.0 39.0 35.0 39.0 10-11 37.49238572770638 39.0 38.0 39.0 35.0 39.0 12-13 37.40234618592936 39.0 37.5 39.0 35.0 39.0 14-15 38.513504539306915 40.0 38.0 41.0 34.0 41.0 16-17 38.50827886974902 40.0 38.0 41.0 34.5 41.0 18-19 38.63983879588911 40.0 38.0 41.0 35.0 41.0 20-21 38.5946142803923 40.0 38.0 41.0 34.5 41.0 22-23 38.50804552669728 40.0 38.0 41.0 34.5 41.0 24-25 38.50063169659127 40.0 38.0 41.0 34.5 41.0 26-27 38.29235151080768 40.0 38.0 41.0 34.0 41.0 28-29 38.12063076524373 40.0 38.0 41.0 34.0 41.0 30-31 37.84943805324069 40.0 37.0 41.0 34.0 41.0 32-33 37.49228702511399 40.0 35.5 41.0 33.0 41.0 34-35 37.14753000558808 40.0 35.0 41.0 33.0 41.0 36-37 36.8110518558112 39.0 35.0 41.0 32.5 41.0 38-39 36.46536348874689 39.0 35.0 41.0 31.5 41.0 40-41 36.25369755096091 39.0 35.0 41.0 31.0 41.0 42-43 36.02378783093208 39.0 35.0 41.0 30.0 41.0 44-45 35.63964493387432 39.0 35.0 41.0 29.5 41.0 46-47 35.04065635705435 38.0 35.0 41.0 25.0 41.0 48-49 34.71301031771099 39.0 35.0 41.0 23.0 41.0 50-51 33.64027713663273 38.0 34.0 40.0 21.0 40.5 52-53 33.76710035067259 38.0 34.0 39.5 20.5 40.5 54-55 34.27273702794852 38.0 35.0 40.5 20.0 41.0 56-57 34.227098973897974 38.0 35.0 41.0 19.5 41.0 58-59 34.08872907505041 38.0 35.0 41.0 17.5 41.0 60-61 33.7443699028969 38.0 34.5 40.0 12.0 41.0 62-63 33.320524773844525 37.5 34.0 40.0 7.5 41.0 64-65 32.86725614891842 37.0 33.5 40.0 2.0 41.0 66-67 32.441561991302024 36.0 33.0 39.0 2.0 41.0 68-69 32.05619467593155 36.0 33.0 39.0 2.0 41.0 70-71 31.572928561594466 35.0 32.5 38.5 2.0 40.0 72-73 31.033724398066035 35.0 32.0 37.0 2.0 39.5 74-75 30.562530370028426 35.0 31.0 37.0 2.0 39.0 76-77 30.041533544708734 35.0 31.0 36.0 2.0 39.0 78-79 29.609291608963613 35.0 30.5 36.0 2.0 37.5 80-81 29.21320468589292 35.0 30.0 35.5 2.0 37.0 82-83 28.777245964835558 34.5 29.5 35.0 2.0 37.0 84-85 28.364136134664754 34.0 29.0 35.0 2.0 36.0 86-87 27.926257521643706 34.0 29.0 35.0 2.0 36.0 88-89 27.429578990419266 34.0 26.5 35.0 2.0 35.5 90-91 26.967894324449087 34.0 25.5 35.0 2.0 35.0 92-93 26.576763790017573 34.0 25.0 35.0 2.0 35.0 94-95 26.135633053119207 34.0 24.0 35.0 2.0 35.0 96-97 25.504569676943884 33.5 19.0 35.0 2.0 35.0 98-99 24.80629135385537 33.0 2.0 35.0 2.0 35.0 100-101 22.469306530770915 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28991720690277134 1101 2 0.3840473770663806 1101 3 0.4074696554428243 1101 4 0.31690948473482905 1101 5 0.36964875196502334 1101 6 0.3551445439965022 1101 7 0.31369779478266224 1101 8 0.4165062961359567 1101 9 0.5679491335096429 1101 10-11 0.48461242517920056 1101 12-13 0.365352128001426 1101 14-15 0.3850572713785425 1101 16-17 0.3637202240055615 1101 18-19 0.5439263125410747 1101 20-21 0.6073480239008759 1101 22-23 0.593831638071002 1101 24-25 0.5898388923965214 1101 26-27 0.7582366284855624 1101 28-29 0.6959027019788593 1101 30-31 0.5141830039014295 1101 32-33 0.5315984581272701 1101 34-35 0.45441976893813774 1101 36-37 0.5997648772308537 1101 38-39 0.7432627299048207 1101 40-41 0.7158784891436056 1101 42-43 0.8200916350272891 1101 44-45 0.724222291582052 1101 46-47 0.7807307838670141 1101 48-49 0.7671358585350561 1101 50-51 0.3907661367507558 1101 52-53 0.46908422438822583 1101 54-55 0.48735627433429585 1101 56-57 0.6371678015230771 1101 58-59 0.6555897983819392 1101 60-61 0.8737304301127296 1101 62-63 0.8767547852196031 1101 64-65 0.9012325154078056 1101 66-67 0.4881248525402171 1101 68-69 0.7394249690180814 1101 70-71 0.6313047219188661 1101 72-73 0.652440363651305 1101 74-75 0.6557742940983253 1101 76-77 0.47112252635771057 1101 78-79 0.3408903323414698 1101 80-81 0.3223392825477198 1101 82-83 0.17962125438812393 1101 84-85 0.34281610401052376 1101 86-87 0.42011831204396444 1101 88-89 0.5473560010328313 1101 90-91 0.48147626463909887 1101 92-93 0.5868293356607026 1101 94-95 0.4660704688174384 1101 96-97 0.6157269699942169 1101 98-99 0.43505768075584683 1101 100-101 0.6458806238332571 1102 1 0.194060129173117 1102 2 0.1918887265079121 1102 3 0.3537541323062854 1102 4 0.19853702063635836 1102 5 0.321928214011983 1102 6 0.30995990757069336 1102 7 0.30516120941680924 1102 8 0.26591363600374507 1102 9 0.30528672183879735 1102 10-11 0.3108746772876998 1102 12-13 0.24929616720817904 1102 14-15 0.2851712444808854 1102 16-17 0.287896768341092 1102 18-19 0.3800529366577834 1102 20-21 0.40161232752522125 1102 22-23 0.5083347153447662 1102 24-25 0.4404030592531356 1102 26-27 0.565793045171219 1102 28-29 0.5515044799592559 1102 30-31 0.5071309181936527 1102 32-33 0.5300883145211586 1102 34-35 0.35457078329874037 1102 36-37 0.4779276308210001 1102 38-39 0.7298737397065054 1102 40-41 0.475059022537728 1102 42-43 0.4899030095429069 1102 44-45 0.49743006454762906 1102 46-47 0.3741972757749181 1102 48-49 0.3055675886467455 1102 50-51 0.2551808848701995 1102 52-53 0.3359095036223252 1102 54-55 0.41168953168556044 1102 56-57 0.36580354348797073 1102 58-59 0.17831603499009674 1102 60-61 0.07227442373024218 1102 62-63 0.12181405123283184 1102 64-65 0.20165136655892724 1102 66-67 0.4431026277852581 1102 68-69 0.32232330501079076 1102 70-71 0.25931646114840134 1102 72-73 0.2962858161245201 1102 74-75 0.2653195414199594 1102 76-77 0.18616982519518288 1102 78-79 0.36510961659038443 1102 80-81 0.46801400331361975 1102 82-83 0.5023731349443139 1102 84-85 0.5403115450864284 1102 86-87 0.48237042054635637 1102 88-89 0.44397099989310007 1102 90-91 0.2681157677163739 1102 92-93 0.17676836005094643 1102 94-95 -0.10027897271435648 1102 96-97 -0.17099801290981986 1102 98-99 -0.15574468988468126 1102 100-101 0.13131486135320003 1103 1 0.17977524243708132 1103 2 0.2912305486619644 1103 3 0.3357632022507815 1103 4 0.3077653029742393 1103 5 0.29156541623202514 1103 6 0.2385465078862694 1103 7 0.11065117399768098 1103 8 0.30354231997907277 1103 9 0.3620704224202882 1103 10-11 0.28666126325907726 1103 12-13 0.3687371022289767 1103 14-15 0.46002559573711466 1103 16-17 0.5211244051902284 1103 18-19 0.5318758906791246 1103 20-21 0.39739251377907436 1103 22-23 0.5380299563824096 1103 24-25 0.5294046481485211 1103 26-27 0.4763018227148308 1103 28-29 0.6423859858789953 1103 30-31 0.6744221549942395 1103 32-33 0.7181751851696276 1103 34-35 0.7543276216176054 1103 36-37 0.6994171650760421 1103 38-39 0.7691237314310087 1103 40-41 0.7237225298036094 1103 42-43 0.9015779425113735 1103 44-45 0.8852605327200678 1103 46-47 0.8105483609683972 1103 48-49 0.7816555406268009 1103 50-51 0.7750096648412921 1103 52-53 0.6641867382823179 1103 54-55 0.7663373265415103 1103 56-57 0.6386839129061457 1103 58-59 0.6713397911829304 1103 60-61 0.63254417734111 1103 62-63 0.5176379596947953 1103 64-65 0.5634153990430164 1103 66-67 0.6272579478263083 1103 68-69 0.6517280761105511 1103 70-71 0.7588826871908338 1103 72-73 0.49405639728507467 1103 74-75 0.3090541627883958 1103 76-77 0.3894346466099634 1103 78-79 0.6530789751842114 1103 80-81 0.7713040649936573 1103 82-83 0.6158157140304787 1103 84-85 0.6580960014966273 1103 86-87 0.3966128263987905 1103 88-89 0.5597548549505333 1103 90-91 0.5635572103009778 1103 92-93 0.5112577343130482 1103 94-95 0.8039142215170685 1103 96-97 0.6243427444636467 1103 98-99 0.5411142073777846 1103 100-101 0.07979299749063884 1104 1 0.14465792322884852 1104 2 0.15052727159505253 1104 3 0.1482519011031158 1104 4 0.07096903208706351 1104 5 0.12985529997645529 1104 6 0.2244479937877557 1104 7 0.1396206688762689 1104 8 0.3214832242836181 1104 9 0.4012495070539188 1104 10-11 0.3146635128522348 1104 12-13 0.17224072391442036 1104 14-15 0.22263967085118708 1104 16-17 0.2830291670949876 1104 18-19 0.23465637186870225 1104 20-21 0.31263257674640244 1104 22-23 0.36439579979370507 1104 24-25 0.39019046347979724 1104 26-27 0.5611096234317188 1104 28-29 0.5773738072304582 1104 30-31 0.39041899599107666 1104 32-33 0.354549457907531 1104 34-35 0.45410665094208724 1104 36-37 0.524067228362469 1104 38-39 0.5677326266308143 1104 40-41 0.455567568466833 1104 42-43 0.2906365702063667 1104 44-45 0.29069119951437017 1104 46-47 0.3693349368004277 1104 48-49 0.38140744719688513 1104 50-51 0.4727810785217983 1104 52-53 0.5650603834741403 1104 54-55 0.48940802120311133 1104 56-57 0.5118684167724723 1104 58-59 0.5617725054792828 1104 60-61 0.4989446687416077 1104 62-63 0.4918201554679058 1104 64-65 0.6244801532895892 1104 66-67 0.4998036044628762 1104 68-69 0.5134911083281324 1104 70-71 0.6451093802357164 1104 72-73 0.689319620957388 1104 74-75 0.6638576823646432 1104 76-77 0.6462186590757959 1104 78-79 0.5802470671250326 1104 80-81 0.6290658261645099 1104 82-83 0.5981254292947362 1104 84-85 0.8098398350586429 1104 86-87 0.7351699490468242 1104 88-89 0.7536925204791061 1104 90-91 0.3579374819539751 1104 92-93 0.5613404321230213 1104 94-95 0.5742390032054843 1104 96-97 0.8278863855072878 1104 98-99 0.5576983091891599 1104 100-101 0.6644040252834849 1105 1 0.36354101655577153 1105 2 0.36898321363275244 1105 3 0.4354804533483545 1105 4 0.39688045518980886 1105 5 0.4163347716617096 1105 6 0.41785107428761137 1105 7 0.45671849529000497 1105 8 0.36164045604262895 1105 9 0.43578738196998756 1105 10-11 0.44125123795220134 1105 12-13 0.5652449628585927 1105 14-15 0.6296440751442418 1105 16-17 0.4152130751409686 1105 18-19 0.6866845262744974 1105 20-21 0.7708732144920489 1105 22-23 0.655037178101928 1105 24-25 0.5895160404472009 1105 26-27 0.6495725301649315 1105 28-29 0.8329906587872458 1105 30-31 0.8317614786294456 1105 32-33 0.8756189144608655 1105 34-35 0.7084771226373618 1105 36-37 0.7578843324521003 1105 38-39 0.7702242589750554 1105 40-41 0.5651500374764424 1105 42-43 0.6247302578873786 1105 44-45 0.652581037071613 1105 46-47 0.8064253225142863 1105 48-49 0.8338583686411312 1105 50-51 0.7885285556986403 1105 52-53 0.7865107687871458 1105 54-55 0.8278151853194018 1105 56-57 0.6869534712373948 1105 58-59 0.6268491922884536 1105 60-61 0.6974647962105607 1105 62-63 0.6693603903635363 1105 64-65 0.7831236410487534 1105 66-67 0.6983713323687581 1105 68-69 0.6847938070223663 1105 70-71 0.489176612094937 1105 72-73 0.588523816810639 1105 74-75 0.6686246155937532 1105 76-77 0.6419715727235875 1105 78-79 0.6754999256565348 1105 80-81 0.5843621263579202 1105 82-83 0.48486081596060515 1105 84-85 0.5988546563315289 1105 86-87 0.6616285266455542 1105 88-89 0.7310228123950502 1105 90-91 0.6406565191224018 1105 92-93 0.696303955156619 1105 94-95 0.4040937467142882 1105 96-97 0.8535223523974267 1105 98-99 0.8184491183965861 1105 100-101 0.8605792049125043 1106 1 0.14405227556438405 1106 2 0.3051377644408362 1106 3 0.28709662818313575 1106 4 0.39093080057823926 1106 5 0.31490905120667634 1106 6 0.3028131236234728 1106 7 0.3860435623579832 1106 8 0.3772974418625381 1106 9 0.37529182668447447 1106 10-11 0.33985940201871756 1106 12-13 0.4974309351340409 1106 14-15 0.6138644039876837 1106 16-17 0.6692336488877046 1106 18-19 0.5997625494748746 1106 20-21 0.5439000837492074 1106 22-23 0.5609048088027109 1106 24-25 0.6297241147781492 1106 26-27 0.5973301927405288 1106 28-29 0.556143017617309 1106 30-31 0.6553236064839254 1106 32-33 0.5751140350897614 1106 34-35 0.6357299759477684 1106 36-37 0.5009485214202343 1106 38-39 0.6847645745451132 1106 40-41 0.49351991156623853 1106 42-43 0.3860733935843754 1106 44-45 0.4131313918075037 1106 46-47 0.3768724112496713 1106 48-49 0.24415727685848054 1106 50-51 0.23594445197929304 1106 52-53 0.33534011698916544 1106 54-55 0.3718177418481261 1106 56-57 0.3218086090656058 1106 58-59 0.5076522087119244 1106 60-61 0.45951132783767434 1106 62-63 0.3867260908150172 1106 64-65 0.5608067109396799 1106 66-67 0.34966922638732 1106 68-69 0.3898348890836729 1106 70-71 0.32575799103138436 1106 72-73 0.5011619254467519 1106 74-75 0.24347669213070944 1106 76-77 0.2555711838694954 1106 78-79 0.302216936153723 1106 80-81 0.37352036473669514 1106 82-83 0.4394362779585279 1106 84-85 0.3554621380338894 1106 86-87 0.21551952130815621 1106 88-89 0.4456497087598237 1106 90-91 0.5693605647005882 1106 92-93 0.5108019734963243 1106 94-95 0.32925452292688817 1106 96-97 0.4430020263582257 1106 98-99 0.24983529912026725 1106 100-101 0.11098874025055139 1107 1 0.03819128710119912 1107 2 0.1277947933540986 1107 3 -0.10528871578495824 1107 4 0.07324991466455089 1107 5 0.083006544441254 1107 6 0.04982209670422577 1107 7 0.04456798907782655 1107 8 0.10140191415993627 1107 9 0.10278790986350828 1107 10-11 0.1421801184408764 1107 12-13 0.11550973445037727 1107 14-15 0.28800270337523415 1107 16-17 0.16103057952437183 1107 18-19 0.25101860274364185 1107 20-21 0.2541933112096828 1107 22-23 0.29183701219253777 1107 24-25 0.24827471298353743 1107 26-27 0.23296792240866182 1107 28-29 0.10783170156546618 1107 30-31 0.23527126351283556 1107 32-33 0.24098250112023578 1107 34-35 0.13443475446933917 1107 36-37 0.14018705581704438 1107 38-39 0.2241019196526466 1107 40-41 0.08459490088399946 1107 42-43 0.08605380947954444 1107 44-45 0.07372105140015606 1107 46-47 0.06077160652100133 1107 48-49 0.1426448498537809 1107 50-51 -0.0117633175692049 1107 52-53 -0.10917900421863891 1107 54-55 -0.11567958759211905 1107 56-57 0.030067760183683845 1107 58-59 0.09121402326825745 1107 60-61 0.018041095677013175 1107 62-63 -0.17804833590482616 1107 64-65 -0.03969090336028103 1107 66-67 -0.2654897609364255 1107 68-69 -0.2885540512382221 1107 70-71 -0.1718474070600564 1107 72-73 -0.25279940484807994 1107 74-75 -0.17921950499220785 1107 76-77 -0.1809496108890869 1107 78-79 -0.26360791073418355 1107 80-81 -0.3459017261335511 1107 82-83 -0.13658120530596207 1107 84-85 -0.3052254420829037 1107 86-87 -0.24661639237934807 1107 88-89 -0.07380976994691224 1107 90-91 -0.07857954354609831 1107 92-93 -0.11242655520329237 1107 94-95 -0.2017907341589975 1107 96-97 -0.4154093466655624 1107 98-99 0.0376904835669265 1107 100-101 0.18672136380845217 1108 1 0.057833481477402415 1108 2 0.17165006279083883 1108 3 0.1650792080764063 1108 4 0.032254483336018325 1108 5 0.0991128787230835 1108 6 0.15692739707007775 1108 7 0.1469234617630022 1108 8 0.206040617965094 1108 9 0.1700918634875137 1108 10-11 0.39092362332768005 1108 12-13 0.3086581632515575 1108 14-15 0.4040487258916272 1108 16-17 0.36783973365738376 1108 18-19 0.28172292486591033 1108 20-21 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-0.7978955229275115 2316 62-63 -1.039195599789693 2316 64-65 -1.144044008508292 2316 66-67 -1.098214903936661 2316 68-69 -0.9831900596205401 2316 70-71 -0.9604014571958075 2316 72-73 -0.8069648633714195 2316 74-75 -0.9308603832904367 2316 76-77 -0.9688397313519594 2316 78-79 -0.6866719708865396 2316 80-81 -0.5580593822476345 2316 82-83 -0.5807806346229896 2316 84-85 -0.5134967207817986 2316 86-87 -0.54637734474775 2316 88-89 -0.5652857696305986 2316 90-91 -0.8740760245540855 2316 92-93 -0.907504579295864 2316 94-95 -0.8613126059464378 2316 96-97 -0.9614816177992829 2316 98-99 -1.039102126549846 2316 100-101 -1.11511869339164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3324.0 3 671.0 4 563.0 5 631.0 6 782.0 7 853.0 8 1125.0 9 1339.0 10 1783.0 11 2585.0 12 4324.0 13 7344.0 14 6857.0 15 8145.0 16 11152.0 17 16655.0 18 15333.0 19 7525.0 20 6859.0 21 6975.0 22 7574.0 23 8428.0 24 9166.0 25 9889.0 26 10860.0 27 12820.0 28 15083.0 29 17003.0 30 20343.0 31 24239.0 32 30267.0 33 39074.0 34 55384.0 35 101561.0 36 126160.0 37 181580.0 38 181485.0 39 32073.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.46078446310037 19.680546879364545 14.558733157608723 17.29993549992636 2 13.856433977791921 24.245440208864583 40.31173606522153 21.586389748121963 3 21.478694100263297 32.08117382606246 30.032775188959 16.40735688471525 4 10.850297216352219 17.991293815974174 39.84564006668998 31.312768900983627 5 10.55076747783529 42.58523758259181 32.70088131717078 14.16311362240212 6 24.335113523875528 38.831457395491064 19.15665372811617 17.676775352517243 7 24.06929661724532 38.945756762672154 20.873695900649313 16.111250719433208 8 24.50070477412268 31.60843540144765 21.087186284315305 22.803673540114364 9 27.98205387485131 19.52593535261015 22.38736557124243 30.10464520129611 10-11 23.22478867074583 28.093644468356082 26.205891382386643 22.47567547851144 12-13 29.34664817814237 27.11221848612626 26.07439216105484 17.46674117467653 14-15 25.861414710684933 26.78186478280408 29.819076439203833 17.537644067307152 16-17 17.668907076032752 30.96673630375007 31.324529747057312 20.039826873159868 18-19 17.244681287647452 30.88199604912301 35.14953091568845 16.72379174754109 20-21 19.615036353511485 28.411831143778908 33.12924966696849 18.84388283574111 22-23 18.51288794971001 28.34036450613296 32.4352688695141 20.71147867464293 24-25 19.27870460987822 28.937569465949075 33.81656884205426 17.967157082118447 26-27 20.280481920871885 26.724541635919852 35.41024700167858 17.584729441529692 28-29 17.83549919572024 27.706246826211718 36.69436456830905 17.763889409759003 30-31 17.667602613353683 30.676754824027324 34.8006970699852 16.854945492633792 32-33 17.20751944465557 30.486787768234908 33.70687340072458 18.59881938638494 34-35 18.20551492992078 28.486491976437133 34.27102376599635 19.036969327645746 36-37 19.60628431393493 28.24212960278489 34.76667287204011 17.384913211240068 38-39 20.525559441732934 27.607532923742216 35.03183806471757 16.835069569807285 40-41 17.607938796551814 29.249324333942738 36.077415366344205 17.065321503161243 42-43 16.84954002370954 31.136394959662756 33.70813259644906 18.305932420178642 44-45 17.7096106614803 31.002486546253884 32.20166403805143 19.08623875421439 46-47 17.90403896547795 30.524127589967286 31.01012793176972 20.56170551278504 48-49 18.680011191087825 30.143245924154943 31.532975557646818 19.64376732711041 50-51 19.508781067400186 30.87828984846759 31.623337782190465 17.98959130194176 52-53 17.65645377546017 31.519390522540046 32.108603609743994 18.715552092255795 54-55 16.38799660672836 30.791397201974934 32.76455007618587 20.05605611511084 56-57 18.636008857679663 32.094088693746485 29.63941311516255 19.6304893334113 58-59 18.33457887860255 30.430246922994797 30.832634302825102 20.40253989557755 60-61 18.41103884818382 29.98525267123824 31.86393632731549 19.739772153262454 62-63 20.731617394665047 29.44349378198951 31.37894648519043 18.445942338155014 64-65 19.437091280515347 30.220622047147966 32.4794902680508 17.862796404285884 66-67 18.035744899054464 30.271706202629716 32.223314814654664 19.469234083661153 68-69 17.619507211617957 32.012623702053766 31.613439572141367 18.75442951418691 70-71 18.381565526170533 31.36572101553418 30.212726990611323 20.039986467683963 72-73 18.189078637533402 30.215935664408235 30.093238587315096 21.50174711074327 74-75 18.315487346035116 31.422266175851142 30.61695836818083 19.64528810993291 76-77 19.284945536517185 29.206678439622237 32.09851237584482 19.409863648015754 78-79 17.87704809657964 29.472656448643757 33.34825015510104 19.30204529967556 80-81 17.75304371528288 31.945572405614442 31.526691276420355 18.774692602682325 82-83 17.63197150240489 30.853864889703548 30.841556890673942 20.67260671721762 84-85 17.77948892958808 29.757444230889536 30.775251794463088 21.687815045059295 86-87 18.912166791191094 29.800111588807958 31.371102974497838 19.91661864550311 88-89 20.112252444903827 28.015754407846483 31.77306775534786 20.09892539190183 90-91 18.304460123632584 28.632883663171153 33.09524572625114 19.967410486945116 92-93 17.968228581660725 30.874019224821343 31.805552164216767 19.352200029301166 94-95 17.900896675291218 30.64669894684149 30.895413657993615 20.556990719873674 96-97 18.035853365341815 29.324031949470147 30.13986159841972 22.500253086768318 98-99 19.075163621454276 29.050400393118792 30.081218098521838 21.793217886905094 100-101 20.34650045929469 28.435529368919944 31.030569800077107 20.18740037170826 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7542.0 1 4838.5 2 3513.0 3 4619.0 4 3449.0 5 3164.0 6 5751.0 7 11140.5 8 15785.0 9 15169.0 10 10514.5 11 6914.0 12 6120.0 13 5872.5 14 5413.5 15 4960.5 16 4420.0 17 4029.5 18 3765.0 19 3647.0 20 3675.0 21 3780.0 22 3975.5 23 4209.0 24 4515.5 25 5116.0 26 6328.5 27 7255.0 28 7462.5 29 8622.5 30 11110.0 31 13593.5 32 17334.5 33 21549.0 34 24153.0 35 25970.5 36 30031.5 37 33681.0 38 32392.0 39 30475.5 40 29257.0 41 27604.5 42 25076.0 43 23901.5 44 24700.5 45 24799.5 46 26562.5 47 35120.5 48 85823.0 49 87298.0 50 41324.0 51 27686.0 52 14199.5 53 10043.5 54 8845.5 55 8253.0 56 7862.0 57 7544.5 58 7520.5 59 7546.5 60 7088.5 61 6573.5 62 5745.0 63 3935.5 64 2535.5 65 2034.5 66 1598.5 67 1165.0 68 818.5 69 579.0 70 497.0 71 434.5 72 358.5 73 359.5 74 297.5 75 188.0 76 138.0 77 96.5 78 83.5 79 59.0 80 36.0 81 28.0 82 25.0 83 25.0 84 16.5 85 13.5 86 8.5 87 2.5 88 2.0 89 2.0 90 3.0 91 2.5 92 2.0 93 2.0 94 3.0 95 2.5 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33619621467965694 2 0.1162159754448197 3 0.1107493703280773 4 0.11753201000996136 5 0.13545032678150587 6 0.08533977987803397 7 0.09394462126549884 8 0.09870259238562648 9 0.08726321501170259 10-11 0.0859471804465609 12-13 0.09085700170882026 14-15 0.09186933598969849 16-17 0.08037934190173068 18-19 0.09581743968512355 20-21 0.10604201592199357 22-23 0.11525425787798538 24-25 0.1287183038136657 26-27 0.12795905310300704 28-29 0.12340354883905505 30-31 0.11282465560387765 32-33 0.10538399863942273 34-35 0.32556670473043564 36-37 0.4856167545372822 38-39 0.4615231986523806 40-41 0.4395555447573233 42-43 0.4725070255999093 44-45 0.47837856442900295 46-47 0.4851105873968431 48-49 0.4951326967775375 50-51 0.4851105873968431 52-53 0.48693278910242394 54-55 0.4751897114442366 56-57 0.4543862419721892 58-59 0.4364173084866007 60-61 0.3957214703952963 62-63 0.4640034176405322 64-65 0.4928043279315176 66-67 0.4995363508993577 68-69 0.5123929962665111 70-71 0.5044967888756611 72-73 0.4968030483409866 74-75 0.5023202701717729 76-77 0.48308591883508667 78-79 0.4642565012107518 80-81 0.46582561934611305 82-83 0.47736623014812474 84-85 0.4780748641447395 86-87 0.4800995327064959 88-89 0.4912858265102003 90-91 0.5045980223037488 92-93 0.49806846619208434 94-95 0.5024215035998607 96-97 0.5012573191768508 98-99 0.49675243162694266 100-101 0.484604420256404 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 987816.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.080074866094137 #Duplication Level Percentage of deduplicated Percentage of total 1 71.70990077623112 17.984896801080584 2 12.353697668146424 6.1966332478040975 3 5.046911159498948 3.797307291682787 4 2.7255101156261525 2.7342399099280317 5 1.6633580025427623 2.0858571616444643 6 1.0948628437760486 1.6475545254004815 7 0.770175236020525 1.3521236805584542 8 0.5799321708081157 1.1635793808899226 9 0.4255951617965781 0.9606562668455061 >10 2.8933008191606753 14.342111762040577 >50 0.41204962231549414 7.24106384250796 >100 0.28184355863238514 13.508843497408861 >500 0.020218332756986018 3.497819209969983 >1k 0.017792132826147698 10.099864230260962 >5k 0.002426199930838322 4.094750606603307 >10k+ 0.002426199930838322 9.292698585374009 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20511 2.0763988435093172 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19300 1.953805162094965 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 14950 1.5134397499129393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14015 1.4187864946508257 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11539 1.1681325267053784 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 11315 1.1454562388137062 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8089 0.8188771998023923 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7210 0.7298930165131967 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6849 0.693347748973493 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 6829 0.6913230804117366 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6193 0.6269386201478818 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5206 0.5270212266252015 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4690 0.47478477773188527 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4610 0.4666861034848595 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4390 0.4444147493055387 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4260 0.4312544036541219 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4238 0.42902726823618975 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4214 0.426597665962082 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3853 0.39005239842237827 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3316 0.33569004753921783 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3088 0.3126088259351944 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3030 0.3067372871061007 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2885 0.29205844003336656 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2877 0.29124857260866394 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2808 0.28426346607060426 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2786 0.2820363306526722 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2722 0.2755573912550516 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2641 0.26735748357993794 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2490 0.2520712359386768 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2489 0.25197000251058904 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2365 0.23941705742769906 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2044 0.20692112701150822 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2020 0.2044915247374005 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1755 0.17766466629412767 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1731 0.17523506402001993 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1719 0.17402026288296607 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1679 0.1699709257594532 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1658 0.16784502376960891 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1650 0.16703515634490634 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1624 0.16440308721462296 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1562 0.15812661467317798 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1517 0.15357111040922602 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1507 0.15255877612834778 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1489 0.150736574422767 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1414 0.14314406731618035 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1379 0.13960089733310657 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1245 0.1260356179693384 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1207 0.1221887477020012 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1151 0.11651967572908314 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1124 0.11378637317071197 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1111 0.11247033860557028 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1104 0.11176170460895551 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1058 0.1071049669169157 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1038 0.10508029835515925 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1035 0.1047765980708958 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1016 0.10285316293722718 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.061671404391101E-5 0.0 14-15 0.0 0.0 0.0 1.0123342808782202E-4 0.0 16-17 0.0 0.0 0.0 1.5185014213173304E-4 0.0 18-19 0.0 0.0 0.0 3.037002842634661E-4 0.0 20-21 0.0 0.0 0.0 6.580172825708431E-4 0.0 22-23 0.0 0.0 0.0 0.0021765187038881736 0.0 24-25 0.0 0.0 0.0 0.004555504263951991 0.0 26-27 0.0 0.0 0.0 0.007896207390850118 0.0 28-29 0.0 0.0 0.0 0.019234351336686183 0.0 30-31 0.0 0.0 0.0 0.04697231063274942 0.0 32-33 0.0 0.0 0.0 0.08326449460223362 0.0 34-35 0.0 0.0 0.0 0.13909473019266747 0.0 36-37 0.0 0.0 0.0 0.22767397976951173 0.0 38-39 0.0 0.0 0.0 0.3488503931906347 0.0 40-41 0.0 0.0 0.0 0.4885525239518291 0.0 42-43 0.0 0.0 0.0 0.617776994905934 0.0 44-45 0.0 5.061671404391101E-5 0.0 0.7537841055419228 0.0 46-47 0.0 1.0123342808782202E-4 0.0 0.9317524721203139 0.0 48-49 0.0 1.0123342808782202E-4 0.0 1.1350798124347046 0.0 50-51 0.0 1.0123342808782202E-4 0.0 1.342912040299003 0.0 52-53 0.0 1.0123342808782202E-4 0.0 1.5440122451954617 0.0 54-55 0.0 1.0123342808782202E-4 0.0 1.7305854531613174 0.0 56-57 0.0 1.0123342808782202E-4 0.0 1.9530459113843062 0.0 58-59 0.0 1.0123342808782202E-4 0.0 2.23872664544814 0.0 60-61 0.0 1.0123342808782202E-4 0.0 2.6259951245981035 0.0 62-63 0.0 1.0123342808782202E-4 0.0 2.965835742688922 0.0 64-65 0.0 1.0123342808782202E-4 0.0 3.262145986701977 0.0 66-67 0.0 1.0123342808782202E-4 0.0 3.5816386857471434 0.0 68-69 0.0 1.0123342808782202E-4 0.0 3.949723430274464 0.0 70-71 0.0 1.0123342808782202E-4 0.0 4.399908484981008 0.0 72-73 0.0 1.0123342808782202E-4 0.0 4.8832981041003585 0.0 74-75 0.0 1.0123342808782202E-4 0.0 5.348313856021769 0.0 76-77 0.0 1.0123342808782202E-4 0.0 5.782099095378086 0.0 78-79 0.0 1.0123342808782202E-4 0.0 6.297427861059145 0.0 80-81 0.0 1.0123342808782202E-4 0.0 6.867220211051451 0.0 82-83 0.0 1.0123342808782202E-4 0.0 7.451742024830535 0.0 84-85 0.0 1.0123342808782202E-4 0.0 8.163261174145791 0.0 86-87 0.0 1.0123342808782202E-4 0.0 8.91932303181969 0.0 88-89 0.0 1.0123342808782202E-4 0.0 9.547425836390584 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGCA 30 1.3180124E-4 63.16856 4 TATCACG 40 5.4574484E-4 47.376415 2 CGCGGAA 30 8.237992E-5 39.62799 82-83 TCGCGGA 30 8.237992E-5 39.62799 82-83 GACATAT 200 0.0 37.97814 1 ATCACGC 50 0.0016365146 37.901134 3 TATAACG 50 0.0016365146 37.901134 2 GCTTATG 150 2.5465852E-11 34.813293 1 CATATAG 210 0.0 33.8403 3 CGATCGT 35 2.0861549E-4 33.838585 24-25 CGTCACT 85 1.6384058E-5 33.510124 1 GTCATAC 65 0.005860125 29.213955 1 CATGGGT 795 0.0 28.60463 4 CATGGGG 2470 0.0 28.003876 4 GGTAATC 120 5.514621E-6 27.620844 8 TACCTGG 470 0.0 27.21624 2 GTACAGG 175 4.989488E-9 27.12724 1 ATGGGGG 1090 0.0 26.948055 5 ATGGGAT 2555 0.0 25.959682 5 GGGCAAC 130 1.027207E-5 25.494873 7 >>END_MODULE