##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939123_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 987816 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38322521603214 33.0 31.0 34.0 31.0 34.0 2 32.55081513156296 34.0 31.0 34.0 31.0 34.0 3 32.61369020141403 34.0 31.0 34.0 31.0 34.0 4 36.0994719664391 37.0 35.0 37.0 35.0 37.0 5 36.09447002275728 37.0 35.0 37.0 35.0 37.0 6 36.19269378102805 37.0 36.0 37.0 35.0 37.0 7 36.145876357540274 37.0 36.0 37.0 35.0 37.0 8 36.16931088380832 37.0 37.0 37.0 35.0 37.0 9 37.83810142779627 39.0 38.0 39.0 35.0 39.0 10-11 37.781993306445735 39.0 38.0 39.0 35.0 39.0 12-13 37.71332515367234 39.0 37.5 39.0 35.0 39.0 14-15 38.8207960794318 40.0 38.0 41.0 35.0 41.0 16-17 38.811690638742434 40.0 38.0 41.0 35.5 41.0 18-19 39.013495428298384 40.0 38.5 41.0 35.0 41.0 20-21 39.121493780218174 41.0 39.0 41.0 35.0 41.0 22-23 39.064938207115496 40.5 38.5 41.0 35.0 41.0 24-25 38.96368908784632 40.5 38.0 41.0 35.0 41.0 26-27 38.67243950290337 40.0 38.0 41.0 34.5 41.0 28-29 38.4481543121391 40.0 38.0 41.0 34.0 41.0 30-31 38.141861945949444 40.0 37.0 41.0 34.0 41.0 32-33 37.79781710359014 40.0 35.5 41.0 33.5 41.0 34-35 37.636597301521746 40.0 35.0 41.0 33.0 41.0 36-37 37.409058468378724 40.0 35.0 41.0 33.0 41.0 38-39 37.15639653538716 40.0 35.0 41.0 33.0 41.0 40-41 36.885805656114094 39.0 35.0 41.0 33.0 41.0 42-43 36.66745881824146 39.0 35.0 41.0 32.0 41.0 44-45 36.29150570551601 39.5 35.0 41.0 31.0 41.0 46-47 35.716562598702595 39.0 35.0 41.0 29.0 41.0 48-49 35.218432886286514 39.0 35.0 41.0 26.0 41.0 50-51 34.934535379058445 39.0 35.0 41.0 24.0 41.0 52-53 34.62183341836941 38.0 35.0 40.0 23.0 41.0 54-55 34.44717032321809 38.0 35.0 40.0 23.0 41.0 56-57 34.252422009767 38.0 35.0 40.0 22.5 41.0 58-59 34.01917411744698 38.0 34.0 40.0 22.0 41.0 60-61 33.5992917709371 37.5 34.0 40.0 19.5 41.0 62-63 33.13556623905667 37.0 33.0 40.0 15.5 41.0 64-65 32.63129064522138 36.0 33.0 39.0 10.0 41.0 66-67 32.25265535281874 36.0 33.0 39.0 7.0 40.0 68-69 31.817805137798942 35.0 32.0 38.5 2.0 40.0 70-71 31.36332576107291 35.0 31.5 37.5 2.0 39.5 72-73 30.8384997813358 35.0 31.0 37.0 2.0 39.0 74-75 30.330636474809076 35.0 31.0 36.5 2.0 39.0 76-77 28.315014132186562 32.5 28.0 35.0 2.0 36.5 78-79 29.206861399288936 34.0 29.0 35.0 2.0 37.0 80-81 29.254021497930786 34.0 30.0 35.0 2.0 37.0 82-83 28.975843679389683 34.0 29.5 35.0 2.0 36.5 84-85 28.59020910776906 34.0 29.0 35.0 2.0 36.0 86-87 28.021644213092316 34.0 29.0 35.0 2.0 36.0 88-89 27.38319636450513 34.0 26.5 35.0 2.0 35.0 90-91 26.8496197672441 33.0 25.5 35.0 2.0 35.0 92-93 26.605388554143687 33.0 25.0 35.0 2.0 35.0 94-95 26.316430387845507 33.0 25.0 35.0 2.0 35.0 96-97 25.69170675510419 33.0 22.0 35.0 2.0 35.0 98-99 24.95973642864663 33.0 10.0 35.0 2.0 35.0 100-101 21.596153028499234 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22514442857936956 1101 2 0.2232002000941904 1101 3 0.24381499086745606 1101 4 0.10056053094670858 1101 5 0.14841910719631102 1101 6 0.17809434359435272 1101 7 0.13216811987922483 1101 8 0.09608562615444072 1101 9 0.24512121590917957 1101 10-11 0.14262754647668885 1101 12-13 0.24517625373044893 1101 14-15 0.2710609702911526 1101 16-17 0.23311432370223173 1101 18-19 0.37290899920387943 1101 20-21 0.31889808096274663 1101 22-23 0.3122216249220173 1101 24-25 0.21317290734099714 1101 26-27 0.19281055873224773 1101 28-29 0.26349127836362385 1101 30-31 0.2606670017459223 1101 32-33 0.4004377509857804 1101 34-35 0.4933140924668322 1101 36-37 0.4021127118957608 1101 38-39 0.5016938389629999 1101 40-41 0.5626204338379281 1101 42-43 0.5427796362834982 1101 44-45 0.6242406201119266 1101 46-47 0.6144385352922512 1101 48-49 0.6720098433471051 1101 50-51 0.6315581749812225 1101 52-53 0.7071709434536544 1101 54-55 0.7284340716446636 1101 56-57 0.6092588246242272 1101 58-59 0.7598406107884159 1101 60-61 0.7594688942151961 1101 62-63 0.8722176369011478 1101 64-65 0.733475174393277 1101 66-67 0.9181091730074513 1101 68-69 0.8427605263362246 1101 70-71 0.747214932474435 1101 72-73 0.5898386016011621 1101 74-75 0.5587444235495873 1101 76-77 0.3851921359063475 1101 78-79 0.5972591738954485 1101 80-81 0.52091030369251 1101 82-83 0.7084471400550356 1101 84-85 0.5926496834670409 1101 86-87 0.5447440553492342 1101 88-89 0.7019210838654466 1101 90-91 0.7641016290942027 1101 92-93 0.9085446729508355 1101 94-95 0.8050140639542143 1101 96-97 0.7782767389495149 1101 98-99 1.0531367254764774 1101 100-101 0.7034571279789503 1102 1 0.12157707047587962 1102 2 0.07506935897269784 1102 3 0.11952732275712918 1102 4 0.07187919974087009 1102 5 -0.0041167943309261545 1102 6 0.09524729549770683 1102 7 0.15002770501713059 1102 8 0.13619276087064947 1102 9 0.262804142738446 1102 10-11 0.2678754379742969 1102 12-13 0.25529136656381723 1102 14-15 0.3141057617203771 1102 16-17 0.341063947590996 1102 18-19 0.25212885331831814 1102 20-21 0.23550396197482115 1102 22-23 0.25801031879026226 1102 24-25 0.34829371882948834 1102 26-27 0.21938623687221082 1102 28-29 0.16601578258062233 1102 30-31 0.38413691284690543 1102 32-33 0.3613022369670915 1102 34-35 0.5035289317647624 1102 36-37 0.6672597371977886 1102 38-39 0.6931971441829674 1102 40-41 0.781502927569413 1102 42-43 0.7057155833999786 1102 44-45 0.6947358332416229 1102 46-47 1.086842797886284 1102 48-49 1.0590539509377166 1102 50-51 1.0328603176755493 1102 52-53 1.1719618028108485 1102 54-55 1.19645891778098 1102 56-57 1.1373474956978527 1102 58-59 1.004136257015908 1102 60-61 0.9283542372742346 1102 62-63 1.165892129720845 1102 64-65 0.9555603123007259 1102 66-67 1.1557687353507333 1102 68-69 1.195470236844546 1102 70-71 1.0334042417974416 1102 72-73 1.04480213020841 1102 74-75 1.2350009770029722 1102 76-77 1.231499407390281 1102 78-79 1.060784931816574 1102 80-81 1.0114134949393758 1102 82-83 1.158025439576349 1102 84-85 1.1760608984203103 1102 86-87 1.1304290336943446 1102 88-89 1.068045428520108 1102 90-91 1.0114147132063493 1102 92-93 0.976683492189494 1102 94-95 1.1184400954107936 1102 96-97 0.8980197296037211 1102 98-99 0.5559832037076156 1102 100-101 0.19586367003502403 1103 1 0.11417601783268339 1103 2 0.103247425595967 1103 3 0.12392988705773433 1103 4 0.12929321732906374 1103 5 0.08799638136338928 1103 6 0.05006859993668655 1103 7 0.15810183140052203 1103 8 0.19289646498301494 1103 9 0.20273920767350972 1103 10-11 0.24626880543945617 1103 12-13 0.1229730688886832 1103 14-15 0.1754408476200311 1103 16-17 0.26343151205711024 1103 18-19 0.28667422510880414 1103 20-21 0.0366796342210165 1103 22-23 0.009767482611998446 1103 24-25 0.09289257791432703 1103 26-27 0.14314891217482284 1103 28-29 0.18606593209126743 1103 30-31 0.17833841016012997 1103 32-33 0.2124212220601649 1103 34-35 0.10997044055334015 1103 36-37 0.1389478833761686 1103 38-39 0.026274856614975306 1103 40-41 0.2556813086615577 1103 42-43 0.2350522195760334 1103 44-45 0.29116261164588764 1103 46-47 0.38425380871063197 1103 48-49 0.5316406781442353 1103 50-51 0.41758057829851936 1103 52-53 0.5712745228011329 1103 54-55 0.7211695512426743 1103 56-57 0.6374854206446159 1103 58-59 0.6538344772323939 1103 60-61 0.7221895438538084 1103 62-63 0.4950500151659014 1103 64-65 0.4637923627481513 1103 66-67 0.34263979759350605 1103 68-69 0.5056172372527961 1103 70-71 0.5011801468609391 1103 72-73 0.419338702387293 1103 74-75 0.2814796151872194 1103 76-77 0.45730461322947136 1103 78-79 0.5733023103598676 1103 80-81 0.6609965478253166 1103 82-83 0.5186646941196003 1103 84-85 0.49043598807152833 1103 86-87 0.7153576989118768 1103 88-89 0.8203411892589436 1103 90-91 0.9775313811603183 1103 92-93 0.9609297047704537 1103 94-95 0.9935849166048918 1103 96-97 0.836718297391073 1103 98-99 0.5820061930377385 1103 100-101 0.4010663369517964 1104 1 0.06096622598653312 1104 2 0.05045245234644824 1104 3 0.11060251009399735 1104 4 0.08449812707942783 1104 5 0.19450238559666388 1104 6 0.06065312188484029 1104 7 0.13978732444964947 1104 8 0.12744686544250783 1104 9 0.20987621481052088 1104 10-11 0.24367951307743851 1104 12-13 0.248829297017636 1104 14-15 0.2239998848154343 1104 16-17 0.20907565088307223 1104 18-19 0.33567267751634233 1104 20-21 0.3292144949376947 1104 22-23 0.18310714458802835 1104 24-25 0.14582781834956648 1104 26-27 0.30726617288298996 1104 28-29 0.3858682284844761 1104 30-31 0.4635866897265899 1104 32-33 0.44773504828307154 1104 34-35 0.5199840551124026 1104 36-37 0.5590095477560126 1104 38-39 0.657318976295457 1104 40-41 0.8061987682699225 1104 42-43 0.7347896340861766 1104 44-45 0.8415637359106469 1104 46-47 1.0234008512667643 1104 48-49 0.9961171823934691 1104 50-51 1.117888102469685 1104 52-53 1.2044352809618957 1104 54-55 0.8844272599549257 1104 56-57 1.1589017602427703 1104 58-59 1.0998043550204457 1104 60-61 1.158820685030399 1104 62-63 0.9277796013045716 1104 64-65 1.1295941592317718 1104 66-67 0.9261074151520319 1104 68-69 0.7994211503748936 1104 70-71 0.8017853565570547 1104 72-73 0.7348122337699152 1104 74-75 0.8987576074652104 1104 76-77 1.0032431137134274 1104 78-79 1.0103881565366244 1104 80-81 0.955007205472338 1104 82-83 1.0232323520963469 1104 84-85 0.7375821158994711 1104 86-87 0.8495973842879287 1104 88-89 0.8358476934018135 1104 90-91 0.5727728002653727 1104 92-93 0.504710350823828 1104 94-95 0.8283468479577323 1104 96-97 0.8723082704810494 1104 98-99 0.7216079405940299 1104 100-101 0.33239856942039125 1105 1 0.03859026270400534 1105 2 0.1317652723967484 1105 3 0.1582931373174361 1105 4 0.10548741447583154 1105 5 0.22739303486662266 1105 6 0.1452353403658364 1105 7 0.0025715491637967602 1105 8 0.11565072558741463 1105 9 0.13224240747704386 1105 10-11 0.21577250428889272 1105 12-13 0.11663912575627933 1105 14-15 0.19685385669610156 1105 16-17 0.2628228073832872 1105 18-19 0.21076474678249468 1105 20-21 0.0868055119836626 1105 22-23 0.017774542798242976 1105 24-25 0.07613514180204817 1105 26-27 0.2635883877563714 1105 28-29 0.20263097287513432 1105 30-31 0.14632789867747675 1105 32-33 0.08202840727054195 1105 34-35 0.1593556291335858 1105 36-37 -0.06452298087940278 1105 38-39 0.11279628341347347 1105 40-41 0.13003205650810656 1105 42-43 -0.03219933764417249 1105 44-45 -0.046453104622877106 1105 46-47 -0.31236699388651346 1105 48-49 -0.1648468266656451 1105 50-51 -0.3312351726407954 1105 52-53 -0.4587227324463967 1105 54-55 -0.3895608549675629 1105 56-57 -0.22725803692926405 1105 58-59 -0.3188966040030934 1105 60-61 -0.05641596429968132 1105 62-63 -0.22661304217316314 1105 64-65 -0.4672946511581557 1105 66-67 -0.28423014710433137 1105 68-69 -0.3311474744234282 1105 70-71 -0.14313342206583712 1105 72-73 -0.21808212332355126 1105 74-75 -0.2523675165464532 1105 76-77 -0.28809661311017365 1105 78-79 -0.11655970344794397 1105 80-81 -0.2687699643560002 1105 82-83 -0.14340111952878232 1105 84-85 0.008361585580498598 1105 86-87 0.006132047427154674 1105 88-89 0.21884707209084553 1105 90-91 0.15194923754803824 1105 92-93 0.17409650561042866 1105 94-95 0.10476446412785023 1105 96-97 0.18317106597955046 1105 98-99 0.2861422329509047 1105 100-101 -0.20769415122016 1106 1 0.11058201824938152 1106 2 0.12383045219503686 1106 3 0.07039318311996112 1106 4 0.0767288031336335 1106 5 0.10840578479417928 1106 6 0.08772746513565721 1106 7 0.1182205896476205 1106 8 0.1400802971935846 1106 9 0.1850730503014688 1106 10-11 0.24323474057383976 1106 12-13 0.24928826438836893 1106 14-15 0.08874645912083423 1106 16-17 0.09829417613100588 1106 18-19 0.08149938657835065 1106 20-21 0.12588721808101155 1106 22-23 0.1413695035856719 1106 24-25 0.16565804773861714 1106 26-27 0.22475614184354242 1106 28-29 0.20507766901744162 1106 30-31 0.2871350279808027 1106 32-33 0.3626226249353621 1106 34-35 0.261235285334223 1106 36-37 0.20310164405399433 1106 38-39 0.22042462395992857 1106 40-41 0.2476685994456389 1106 42-43 0.2602883470848951 1106 44-45 0.38291811550032406 1106 46-47 0.32527835902661906 1106 48-49 0.3104577834987694 1106 50-51 0.3852910095784736 1106 52-53 0.4613623761242849 1106 54-55 0.3091140265534591 1106 56-57 0.5569069082479174 1106 58-59 0.40945118672610903 1106 60-61 0.3358779944406507 1106 62-63 0.3751024493627213 1106 64-65 0.47827687556154785 1106 66-67 0.5409391586602439 1106 68-69 0.42911018815216906 1106 70-71 0.37580685704914174 1106 72-73 0.3091791426956476 1106 74-75 0.20479894291830902 1106 76-77 0.20463515701723267 1106 78-79 0.25799395561301 1106 80-81 0.2191169034055669 1106 82-83 0.21360444688020408 1106 84-85 0.2904438011140975 1106 86-87 0.6108539139268139 1106 88-89 0.7801517663823567 1106 90-91 0.9620007887580684 1106 92-93 0.9926115924015413 1106 94-95 0.8350278987564295 1106 96-97 1.137520436428506 1106 98-99 1.3587398203729713 1106 100-101 0.823730237222648 1107 1 -0.03442047427305539 1107 2 -0.04184217278716318 1107 3 -0.0592802964413508 1107 4 -0.02868709203799824 1107 5 0.037226465608881654 1107 6 0.06693270655425465 1107 7 0.01892917816777384 1107 8 0.006311959349972085 1107 9 0.08801600714522806 1107 10-11 0.08372665767966225 1107 12-13 0.029888428182701432 1107 14-15 0.06022488099805656 1107 16-17 0.035380239369857236 1107 18-19 -0.1482800251863594 1107 20-21 -0.04379569745808709 1107 22-23 0.12701079400014947 1107 24-25 -0.06330239236962854 1107 26-27 0.017694291679752894 1107 28-29 0.021952936073418527 1107 30-31 -0.05943686348764032 1107 32-33 -0.13610885918371451 1107 34-35 -0.07188588686346975 1107 36-37 -0.06205378252341376 1107 38-39 -0.058248285881980166 1107 40-41 -0.16268961163540752 1107 42-43 0.034501422145424954 1107 44-45 -0.26151020667600733 1107 46-47 -0.4416796953815805 1107 48-49 -0.5645600202328822 1107 50-51 -0.4990381458124986 1107 52-53 -0.5421004496839998 1107 54-55 -0.6213178919766662 1107 56-57 -0.5706657309772609 1107 58-59 -0.5206291081036838 1107 60-61 -0.5707992951192438 1107 62-63 -0.782037737220385 1107 64-65 -0.6498521302780773 1107 66-67 -0.5772375611802332 1107 68-69 -0.6730698787898639 1107 70-71 -0.6702380439621081 1107 72-73 -0.9054047840954063 1107 74-75 -0.6277970191953521 1107 76-77 -0.7816381658712466 1107 78-79 -0.7627785483038103 1107 80-81 -0.796710614042091 1107 82-83 -0.6775568905361169 1107 84-85 -0.6890240453465175 1107 86-87 -0.7767911864862356 1107 88-89 -0.41596078880921183 1107 90-91 -0.2832500658210506 1107 92-93 -0.3059525366399072 1107 94-95 -0.5182470976828242 1107 96-97 -0.38062156613523257 1107 98-99 -0.3436930595586638 1107 100-101 -0.38552592286850995 1108 1 -0.052716147732340346 1108 2 0.0102700645363214 1108 3 -0.05045114001075035 1108 4 0.02114854183203363 1108 5 0.003524781297890911 1108 6 -0.019752753229063558 1108 7 -0.06945959191405393 1108 8 -0.006303803892102167 1108 9 0.019973068702995533 1108 10-11 0.04993826139822488 1108 12-13 -0.07594712173688833 1108 14-15 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257.0 8 434.0 9 738.0 10 1329.0 11 2196.0 12 3409.0 13 5145.0 14 5794.0 15 7994.0 16 11401.0 17 17280.0 18 15827.0 19 8555.0 20 6880.0 21 6990.0 22 7690.0 23 8509.0 24 9481.0 25 10318.0 26 11522.0 27 13285.0 28 15858.0 29 18467.0 30 21934.0 31 26453.0 32 32541.0 33 41147.0 34 59178.0 35 104634.0 36 128676.0 37 198243.0 38 170505.0 39 14974.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.74651235075787 20.085852952947462 15.049864191436239 17.11777050485843 2 14.263891251002212 24.68364553722556 39.687046980409306 21.36541623136293 3 21.390522121528704 31.802481433789286 30.482701231808353 16.32429521287365 4 11.02189071648971 18.54059865400034 39.33758918665015 31.099921442859802 5 10.49203495387805 42.6473148845534 32.810462677259736 14.050187484308818 6 24.340666682864015 39.16964292945245 18.72929776395604 17.760392623727494 7 23.830146505017126 39.02001992273865 20.754472492852923 16.395361079391304 8 24.558622253537095 32.06153777626602 20.698895340832706 22.680944629364173 9 27.473031414757404 19.64211958502393 22.18247122945974 30.702377770758925 10-11 23.203764668723732 28.27636928334832 25.96925945722685 22.550606590701104 12-13 29.36305951715704 27.180061873871246 26.05561157138576 17.401267037585946 14-15 25.64763443042742 26.995469254352955 29.76164866297195 17.595247652247682 16-17 17.47001673413977 31.077160278234178 31.406781325388412 20.046041662237638 18-19 17.146268711110526 30.7051772319831 35.30917463838136 16.83937941852502 20-21 19.407511115430403 28.265689156685053 33.5534654227103 18.773334305174245 22-23 18.36834997610891 28.116420466969554 32.84214874025122 20.67308081667031 24-25 19.163773471828744 28.73781454161316 34.21459567233056 17.883816314227534 26-27 20.191333793951728 26.57702324098188 35.839806034100945 17.39183693096545 28-29 17.727238676028733 27.545514549268287 37.09207990152012 17.635166873182857 30-31 17.512193936743383 30.447485588983845 35.23116343013567 16.80915704413709 32-33 17.125912113187074 30.22359427261757 34.208040768726164 18.442452845469195 34-35 18.154753769068076 28.20971923973713 34.685905087621784 18.949621903573007 36-37 19.472377138199114 27.966593842796637 35.23083816645866 17.330190852545588 38-39 20.404909665118474 27.285862841647905 35.52252271408653 16.786704779147087 40-41 17.523762853405366 28.982682580117363 36.47532186668503 17.01823269979224 42-43 16.797240038675906 30.82935521593204 34.17446681414809 18.198937931243957 44-45 17.625027840207334 30.711089513859363 32.65236186195307 19.011520783980238 46-47 17.855716525182007 30.324099500049854 31.33959000747622 20.480593967291913 48-49 18.697782056616322 29.801975487114383 31.8326859396623 19.667556516606986 50-51 19.4149404645004 30.647520687092918 31.825728325372992 18.11181052303369 52-53 17.635521190181166 31.32805097305571 32.264662649724244 18.771765187038884 54-55 16.35142577160119 30.583327259327646 32.89403087214623 20.171216096924933 56-57 18.607564566680434 31.811086275176752 29.83273200677049 19.74861715137232 58-59 18.363927561937622 30.26935474726859 30.943146568829928 20.423571121963864 60-61 18.28086376473816 29.896339929300357 31.8911268052682 19.93166950069328 62-63 20.585957431299658 29.363170299098574 31.448933352771096 18.601938916830672 64-65 19.306521237350523 30.09572904307402 32.45559775984321 18.14215195973225 66-67 17.89078480671779 30.16054923967278 32.242723601907535 19.705942351701896 68-69 17.5440168337846 31.798723495369142 31.6057140514216 19.051545619424655 70-71 18.262814127327356 31.183489637746302 30.252496416336644 20.301199818589698 72-73 18.029167375300663 29.96139969387011 30.13815325931145 21.871279671517772 74-75 18.082416158474857 31.330480575329815 30.482397531524093 20.104705734671235 76-77 19.02616509835689 28.921353546615347 32.26585805422729 19.78662330080048 78-79 17.643759788056624 29.11604547833543 33.440253246825556 19.79994148678239 80-81 17.457199174304215 31.575625168940636 31.579624063423477 19.38755159333167 82-83 17.252441770060578 30.66061420842917 30.997015614370405 21.089928407139848 84-85 17.346246669420214 29.44799436332272 30.849267474914356 22.356491492342702 86-87 18.38715692464224 29.471215771003056 31.441324397809833 20.700302906544866 88-89 19.47050384864976 27.755658127493543 31.898489353517295 20.875348670339402 90-91 17.754974081627882 28.30701864029405 33.07719823464434 20.860809043433726 92-93 17.18194481563368 30.53630433198086 32.02701717728807 20.254733675097388 94-95 17.05444131295707 30.26854191468857 31.04161098828122 21.635405784073146 96-97 16.960395458263484 29.230899276788392 30.28473926318262 23.523966001765512 98-99 18.065134972337376 28.790994082780337 30.167797974276038 22.97607297060625 100-101 19.02815789966537 28.37312443909701 31.15672180072069 21.441995860516936 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6686.0 1 4428.0 2 3528.5 3 4628.5 4 3449.5 5 3110.0 6 5684.5 7 11089.5 8 15579.0 9 15044.5 10 10505.5 11 6853.5 12 5953.0 13 5568.5 14 5090.0 15 4478.5 16 3968.0 17 3692.0 18 3508.5 19 3471.5 20 3521.0 21 3636.0 22 3828.0 23 4069.5 24 4253.0 25 4797.5 26 6117.5 27 7122.5 28 7311.0 29 8403.5 30 10908.0 31 13379.0 32 17049.0 33 21357.0 34 23856.5 35 25953.5 36 30296.0 37 33792.5 38 32839.5 39 31137.0 40 30107.0 41 28556.0 42 25748.5 43 24406.5 44 25295.5 45 25557.5 46 26619.0 47 35611.0 48 86090.5 49 86961.0 50 43276.5 51 29867.5 52 14497.0 53 9565.5 54 8308.5 55 7901.0 56 7650.5 57 7450.0 58 7431.0 59 7410.0 60 6954.5 61 6381.0 62 5538.0 63 3790.5 64 2389.0 65 1861.0 66 1434.0 67 1061.5 68 752.5 69 514.5 70 449.0 71 392.5 72 295.0 73 302.0 74 267.0 75 168.0 76 124.5 77 79.5 78 56.0 79 36.0 80 20.0 81 15.5 82 11.5 83 5.5 84 2.0 85 0.0 86 1.0 87 1.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3383221166695012 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.617175668343092E-4 16-17 0.0015185014213173302 18-19 5.0616714043911006E-5 20-21 0.0 22-23 0.0 24-25 9.617175668343092E-4 26-27 0.0013666512791855971 28-29 0.0 30-31 0.0030370028426346604 32-33 0.0 34-35 0.010629509949221313 36-37 0.027687342582019325 38-39 0.02156272018270609 40-41 0.014172679932295082 42-43 0.012249244798626465 44-45 0.0036444034111615927 46-47 0.0017715849915368852 48-49 9.111008527903982E-4 50-51 2.0246685617564402E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.004859204548215457 60-61 0.011793694372231267 62-63 0.004859204548215457 64-65 0.002834535986459017 66-67 0.005112288118435012 68-69 0.004099953837556792 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.21203341512994323 78-79 5.061671404391102E-4 80-81 0.004353037407776347 82-83 8.098674247025761E-4 84-85 0.0 86-87 0.10558646549559836 88-89 0.394759752828462 90-91 0.38114385675064993 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0021259019898442625 100-101 0.0011641844230099532 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 987816.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.914278788281067 #Duplication Level Percentage of deduplicated Percentage of total 1 70.31215439544636 16.81464462417366 2 12.846251212615249 6.144176657655495 3 5.333996072416039 3.8267600739406022 4 2.857839966753377 2.7337272678892863 5 1.7746732302616683 2.1220015193288426 6 1.154247491984522 1.6561797788395245 7 0.8138158332481225 1.3623273103028999 8 0.6049889230781553 1.157429901625035 9 0.46363002136687304 0.9978639827025699 >10 3.057486555637189 14.50768773406985 >50 0.4334191266149559 7.243490142405072 >100 0.30220825796878376 13.9174165551192 >500 0.02158630414062741 3.597755980386167 >1k 0.018623478082109922 10.261965298207596 >5k 0.002539565193014989 4.249510165556844 >10k+ 0.002539565193014989 9.407063007797356 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20487 2.0739692412352095 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19315 1.9553236635162823 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 15471 1.5661823659466945 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15000 1.5185014213173305 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12062 1.2210776095953093 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 10602 1.073276804587089 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8253 0.8354794820087951 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7705 0.7800035634166688 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7049 0.7135944345910574 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6896 0.6981057200936207 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6384 0.6462742049126559 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5696 0.5766256063882342 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4656 0.47134284117689934 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4641 0.469824339755582 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4473 0.45281712383682793 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4409 0.44633818443920736 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4162 0.4213335277015152 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4141 0.41920762571167103 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3877 0.392482000696486 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3324 0.3364999149639204 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3084 0.3122038922228431 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2995 0.30319411712302696 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2965 0.30015711428039227 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2943 0.2979299788624602 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2932 0.29681641115349416 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2892 0.2927670740299813 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2740 0.2773795929606323 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2552 0.2583477084801218 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2535 0.2566267402026288 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2438 0.2468070976781101 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2378 0.2407330919928408 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2240 0.2267628789167213 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2059 0.20843962843282554 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1889 0.1912299456578958 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1802 0.1824226374142553 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1758 0.1779683665783911 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1711 0.17321039545826347 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1693 0.1713881937526827 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1672 0.1692622917628384 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1629 0.16490925435506207 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1605 0.16247965208095436 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1516 0.15346987698113818 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1502 0.15205260898790868 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1491 0.15093904127894264 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1478 0.14962300671380094 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1451 0.14688970415542976 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1390 0.1407144650420726 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1299 0.1315022230860808 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1248 0.1263393182536019 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1208 0.12228998113008901 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1161 0.11753201000996136 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1159 0.11732954315378573 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1129 0.11429254031115108 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1066 0.10791483434161826 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1052 0.10649756634838878 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1038 0.10508029835515925 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 995 0.10072726094738291 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.061671404391101E-5 0.0 14-15 0.0 0.0 0.0 1.0123342808782202E-4 0.0 16-17 0.0 0.0 0.0 2.5308357021955503E-4 0.0 18-19 0.0 0.0 0.0 4.555504263951991E-4 0.0 20-21 0.0 0.0 0.0 8.604841387464872E-4 0.0 22-23 0.0 0.0 0.0 0.0025308357021955503 0.0 24-25 0.0 0.0 0.0 0.004960437976303279 0.0 26-27 0.0 0.0 0.0 0.008402374531289229 0.0 28-29 0.0 0.0 0.0 0.019689901763081386 0.0 30-31 0.0 0.0 0.0 0.047731561343408085 0.0 32-33 0.0 0.0 0.0 0.08488422945163876 0.0 34-35 0.0 0.0 0.0 0.14187864946508255 0.0 36-37 0.0 0.0 0.0 0.23096406618236592 0.0 38-39 0.0 0.0 0.0 0.3533552807405428 0.0 40-41 0.0 0.0 0.0 0.4935129619281323 0.0 42-43 0.0 0.0 0.0 0.6237497671631154 0.0 44-45 0.0 0.0 0.0 0.7609210622221142 0.0 46-47 0.0 0.0 0.0 0.9411671809324813 0.0 48-49 0.0 0.0 0.0 1.14667103995076 0.0 50-51 0.0 0.0 0.0 1.3567810199470345 0.0 52-53 0.0 0.0 0.0 1.5593491095507666 0.0 54-55 0.0 0.0 0.0 1.7471877353677203 0.0 56-57 0.0 0.0 0.0 1.9710148448698948 0.0 58-59 0.0 0.0 0.0 2.2597325817763636 0.0 60-61 0.0 0.0 0.0 2.648722029203819 0.0 62-63 0.0 0.0 0.0 2.9909922495687455 0.0 64-65 0.0 0.0 0.0 3.2888716117171617 0.0 66-67 0.0 0.0 0.0 3.608465544190416 0.0 68-69 0.0 0.0 0.0 3.9778663232828784 0.0 70-71 0.0 0.0 0.0 4.430430363549487 0.0 72-73 0.0 0.0 0.0 4.916198968228901 0.0 74-75 0.0 0.0 0.0 5.3845554232772095 0.0 76-77 0.0 0.0 0.0 5.82122581533403 0.0 78-79 0.0 0.0 0.0 6.339844667427942 0.0 80-81 0.0 0.0 0.0 6.913382654259498 0.0 82-83 0.0 0.0 0.0 7.501751338305919 0.0 84-85 0.0 0.0 0.0 8.213928504903748 0.0 86-87 0.0 0.0 0.0 8.968927411582724 0.0 88-89 0.0 0.0 0.0 9.59733391643788 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATAGT 90 3.6379788E-12 52.713264 6 GAGTACG 55 8.087445E-7 51.912502 1 TATCACG 60 1.5046971E-6 47.44194 2 GATAGTC 100 1.2732926E-11 47.44194 7 TCACGCA 65 2.6108846E-6 43.79256 4 ATCACGC 65 2.6108846E-6 43.79256 3 CGTCACT 95 1.897206E-8 40.072815 1 CGACCGT 50 3.0648152E-8 37.954514 18-19 GGTAATC 95 8.781899E-7 34.957222 8 CTCTAGG 110 6.8997906E-8 34.608337 1 ACAACGC 55 0.0025949676 34.50323 3 CATGGGT 785 0.0 33.23958 4 TAGTCAA 150 9.658834E-10 31.631165 9 TATACGA 60 0.003974409 31.62796 2 GCTTATG 110 2.694458E-6 30.282293 1 CGTATCA 40 4.348905E-4 29.855223 86-87 TCGACCG 65 3.0707815E-7 29.19578 18-19 GACAATG 400 0.0 27.279116 7 CGGCTCG 70 0.008441216 27.109682 8 TATCGCG 70 0.008441216 27.109682 5 >>END_MODULE