##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939121_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1056915 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.200700150910905 33.0 31.0 34.0 31.0 34.0 2 32.24436212940492 34.0 31.0 34.0 30.0 34.0 3 32.24578608497372 34.0 31.0 34.0 30.0 34.0 4 35.735115879706505 37.0 35.0 37.0 35.0 37.0 5 35.82442391299206 37.0 35.0 37.0 35.0 37.0 6 35.96377381340978 37.0 37.0 37.0 35.0 37.0 7 35.92444236291471 37.0 37.0 37.0 35.0 37.0 8 35.93398617675026 37.0 37.0 37.0 35.0 37.0 9 37.5835332074954 39.0 38.0 39.0 35.0 39.0 10-11 37.519290576820275 39.0 38.0 39.0 35.0 39.0 12-13 37.443178968980476 39.0 38.0 39.0 35.0 39.0 14-15 38.616225997360246 40.0 38.0 41.0 34.5 41.0 16-17 38.603981398693364 40.0 38.0 41.0 34.5 41.0 18-19 38.73898421348926 40.0 38.5 41.0 35.0 41.0 20-21 38.736159956098646 40.0 38.5 41.0 35.0 41.0 22-23 38.63997814393778 40.0 38.0 41.0 35.0 41.0 24-25 38.62105230789609 41.0 38.0 41.0 35.0 41.0 26-27 38.47931385210731 40.0 38.0 41.0 34.5 41.0 28-29 38.3304031071562 40.0 38.0 41.0 34.0 41.0 30-31 37.99977481632865 40.0 37.5 41.0 34.0 41.0 32-33 37.59560986455865 40.0 35.5 41.0 33.5 41.0 34-35 37.19260915021549 40.0 35.0 41.0 33.0 41.0 36-37 36.84031686559468 40.0 35.0 41.0 32.5 41.0 38-39 36.51453096985094 39.5 35.0 41.0 31.5 41.0 40-41 36.269556208398974 39.0 35.0 41.0 30.5 41.0 42-43 35.885229181154585 39.0 35.0 41.0 30.0 41.0 44-45 35.26856133180057 39.0 35.0 41.0 25.5 41.0 46-47 34.38257286536761 39.0 35.0 41.0 14.5 41.0 48-49 33.77937014802515 39.0 35.0 41.0 2.0 41.0 50-51 32.67435981133771 38.0 34.0 40.0 2.0 40.5 52-53 32.78784197404711 38.5 33.5 40.0 2.0 40.5 54-55 33.2574804974856 39.0 35.0 41.0 2.0 41.0 56-57 33.16611506128686 39.0 34.5 41.0 2.0 41.0 58-59 33.001876215211254 39.0 34.0 41.0 2.0 41.0 60-61 32.70471135332548 39.0 34.0 41.0 2.0 41.0 62-63 32.35413491151133 38.0 33.5 40.5 2.0 41.0 64-65 31.987488587067077 38.0 33.0 40.0 2.0 41.0 66-67 31.588327348935344 37.0 32.5 40.0 2.0 41.0 68-69 31.170450320035194 37.0 32.0 39.5 2.0 41.0 70-71 30.74140966870562 36.0 31.5 39.0 2.0 41.0 72-73 30.251799340533534 35.5 31.0 39.0 2.0 40.5 74-75 29.755477971265428 35.0 30.5 37.5 2.0 40.0 76-77 29.271701130176034 35.0 29.5 37.0 2.0 39.5 78-79 28.80098115742515 35.0 29.0 37.0 2.0 39.0 80-81 28.320173334657945 35.0 29.0 36.0 2.0 39.0 82-83 27.820957219833197 35.0 27.5 36.0 2.0 37.5 84-85 27.36832857893019 34.5 26.0 35.0 2.0 37.0 86-87 26.948268309182858 34.0 25.5 35.0 2.0 36.5 88-89 26.449245208933547 34.0 23.5 35.0 2.0 36.0 90-91 25.99502514393305 34.0 19.5 35.0 2.0 36.0 92-93 25.570153228973002 34.0 12.0 35.0 2.0 35.5 94-95 25.088609774674406 34.0 2.0 35.0 2.0 35.0 96-97 24.513223863792263 33.5 2.0 35.0 2.0 35.0 98-99 23.899629109247194 33.0 2.0 35.0 2.0 35.0 100-101 21.75673303908072 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1259597870719702 1101 2 0.3338050473455425 1101 3 0.2370857729916267 1101 4 0.12508913909286434 1101 5 0.2491634320714624 1101 6 0.31162694722603845 1101 7 0.10188165188358056 1101 8 0.3649010824098582 1101 9 0.39141064124758884 1101 10-11 0.3428841163126677 1101 12-13 0.29102663601157985 1101 14-15 0.3507548374733531 1101 16-17 0.46999235858270794 1101 18-19 0.5143522627352013 1101 20-21 0.49944661717886873 1101 22-23 0.6060233404638993 1101 24-25 0.4773982857145356 1101 26-27 0.42380742381655523 1101 28-29 0.5280836184141791 1101 30-31 0.35910750661950175 1101 32-33 0.2904788900529738 1101 34-35 0.35432527643455103 1101 36-37 0.3789671406291788 1101 38-39 0.5742276410719711 1101 40-41 0.42778500459269964 1101 42-43 0.44013986292404894 1101 44-45 0.32001494215896287 1101 46-47 0.2618779141214418 1101 48-49 0.36126893183753594 1101 50-51 0.24304058194923783 1101 52-53 0.3936719701509688 1101 54-55 0.36945327030280595 1101 56-57 0.2519279968197097 1101 58-59 0.07783476058375527 1101 60-61 0.114668552905556 1101 62-63 0.12914611308671198 1101 64-65 0.1935409294150503 1101 66-67 0.09093250428274047 1101 68-69 0.20367724280407629 1101 70-71 0.13604417614086017 1101 72-73 -0.11187281449538133 1101 74-75 0.03833920106277944 1101 76-77 0.1168406733429812 1101 78-79 -0.01207555721842013 1101 80-81 -0.17121518674104053 1101 82-83 -0.11093717713115225 1101 84-85 0.11311842519225124 1101 86-87 -0.1639813281633593 1101 88-89 -0.11662533678928 1101 90-91 -0.32603951087481775 1101 92-93 -0.5303489702253543 1101 94-95 -0.5245670018953206 1101 96-97 -0.4123756567516885 1101 98-99 -0.8019333094043652 1101 100-101 -0.8051919315012732 1102 1 0.15069152592135993 1102 2 0.1827604448891762 1102 3 0.2636287594169673 1102 4 0.11495209966170705 1102 5 0.2516611696280222 1102 6 0.3471098172971452 1102 7 0.21458494858687516 1102 8 0.20217580768458276 1102 9 0.3326336535294274 1102 10-11 0.203181466021789 1102 12-13 0.23178940265670178 1102 14-15 0.3269177334009541 1102 16-17 0.3834506649821918 1102 18-19 0.3184828380422502 1102 20-21 0.35750220864622406 1102 22-23 0.33094512456215597 1102 24-25 0.3379057194572681 1102 26-27 0.22981905923995072 1102 28-29 0.1779795460159832 1102 30-31 0.44613918082765025 1102 32-33 0.26689065475886053 1102 34-35 0.31174285885213493 1102 36-37 0.23961096739065368 1102 38-39 0.2278727606582649 1102 40-41 0.050428831354174974 1102 42-43 -0.060085088595023706 1102 44-45 0.04607570492561308 1102 46-47 0.2114958843864656 1102 48-49 0.24818554463649178 1102 50-51 -0.002499818826457556 1102 52-53 0.05226925521883885 1102 54-55 0.08511778226143463 1102 56-57 0.15632101556567335 1102 58-59 0.18148957635622054 1102 60-61 0.00900016247245361 1102 62-63 -0.20513572272454184 1102 64-65 0.015524122692362141 1102 66-67 0.22636883266024554 1102 68-69 0.14367336432961153 1102 70-71 -0.04213746574666288 1102 72-73 -0.17611004785026552 1102 74-75 -0.23864528503935034 1102 76-77 -0.23956074876432965 1102 78-79 -0.29892688236386533 1102 80-81 -0.14333929792397626 1102 82-83 -0.22515049322682046 1102 84-85 -0.393995989804516 1102 86-87 -0.39810867140834816 1102 88-89 -0.4033066554705975 1102 90-91 -0.33117848954320905 1102 92-93 -0.21226493661190915 1102 94-95 -0.30582556685977025 1102 96-97 -0.2599794059436711 1102 98-99 -0.034828590593765085 1102 100-101 -0.24514991469455083 1103 1 0.08584060877496569 1103 2 0.059278467449040306 1103 3 0.19192221646034113 1103 4 0.1483113438913577 1103 5 0.041514694420527576 1103 6 0.17492818605307292 1103 7 0.12440509550911116 1103 8 0.0955305319942994 1103 9 0.2499886559880622 1103 10-11 0.24959259992565563 1103 12-13 0.19886104090416268 1103 14-15 0.11553200542066833 1103 16-17 0.20104457749442872 1103 18-19 0.21402922682489844 1103 20-21 0.13949554300606337 1103 22-23 0.3346988932316606 1103 24-25 0.3245489835348252 1103 26-27 0.1582279977541461 1103 28-29 0.12361819580166156 1103 30-31 0.07946315974793805 1103 32-33 0.1852011732582426 1103 34-35 0.11275400467667396 1103 36-37 -0.03789721565119919 1103 38-39 0.04820035551449564 1103 40-41 0.012492547324399084 1103 42-43 -0.13482767667137807 1103 44-45 -0.24930354615842276 1103 46-47 -0.23773883356555814 1103 48-49 -2.709772106115338E-4 1103 50-51 0.14509233042030445 1103 52-53 0.04987394600168216 1103 54-55 0.12641234196659923 1103 56-57 0.09159178857132844 1103 58-59 0.10420515418575604 1103 60-61 0.21385249519816796 1103 62-63 0.039661402407709545 1103 64-65 -0.03639038625062341 1103 66-67 0.031550349664581034 1103 68-69 0.07395856631042719 1103 70-71 0.11787701289795649 1103 72-73 0.0998254762369335 1103 74-75 0.1163664866202545 1103 76-77 0.213728238028569 1103 78-79 0.22194608305130004 1103 80-81 0.17134150135681026 1103 82-83 0.05277491319345273 1103 84-85 0.18210133251544391 1103 86-87 0.22913142077814896 1103 88-89 0.2739288422917916 1103 90-91 0.338426538991893 1103 92-93 0.2141572239098224 1103 94-95 0.18945024759503326 1103 96-97 0.018330317844519328 1103 98-99 -0.3233578036806719 1103 100-101 -0.1138543119309432 1104 1 0.2768407133745896 1104 2 0.1680644746652078 1104 3 0.19310767879666457 1104 4 0.19561081019285353 1104 5 0.2049750421481349 1104 6 0.2600193740763004 1104 7 0.24474755706273754 1104 8 0.25643480170261057 1104 9 0.2568802078255885 1104 10-11 0.26318453878176484 1104 12-13 0.14810377573931532 1104 14-15 0.31811080680522963 1104 16-17 0.22220015079049915 1104 18-19 0.2348529668503616 1104 20-21 0.2147080786090072 1104 22-23 0.2366151101400078 1104 24-25 0.34263690251941625 1104 26-27 0.48095028095941217 1104 28-29 0.4117156008895293 1104 30-31 0.37165077058445206 1104 32-33 0.40683814525560535 1104 34-35 0.5370040434506649 1104 36-37 0.605418087272902 1104 38-39 0.6837867085105529 1104 40-41 0.6859511757709313 1104 42-43 0.6170223539270197 1104 44-45 0.8480462361661694 1104 46-47 1.0625432247144602 1104 48-49 0.9579955786846597 1104 50-51 1.0397190360252822 1104 52-53 0.8941100862517359 1104 54-55 0.8369112580981408 1104 56-57 0.8122140240455096 1104 58-59 0.8487332115303943 1104 60-61 0.992773387829665 1104 62-63 1.021236239827438 1104 64-65 1.0025417117652324 1104 66-67 0.9622452878846843 1104 68-69 0.9665894631138876 1104 70-71 0.8711691956996148 1104 72-73 0.9971868005883273 1104 74-75 0.9238250405245232 1104 76-77 0.8802291884423354 1104 78-79 0.8757898785506271 1104 80-81 0.8361971118037879 1104 82-83 0.7091122674002222 1104 84-85 0.8331515968398833 1104 86-87 0.6915248524030595 1104 88-89 0.832602014173009 1104 90-91 0.7812832868094297 1104 92-93 0.9304127259098394 1104 94-95 1.0059139869953029 1104 96-97 0.9799566856047512 1104 98-99 0.9913710248156313 1104 100-101 0.9377716109803416 1105 1 0.19361596650897184 1105 2 0.2364237437176584 1105 3 0.2631173852150255 1105 4 0.29425849028961437 1105 5 0.1927756554645086 1105 6 0.2993505660729454 1105 7 0.26832572835505175 1105 8 0.31492607111977833 1105 9 0.3317038822531586 1105 10-11 0.2846604221985629 1105 12-13 0.3606247091712973 1105 14-15 0.4265272294649307 1105 16-17 0.4686796986399173 1105 18-19 0.5377693935495884 1105 20-21 0.4105301637692662 1105 22-23 0.47646982548572936 1105 24-25 0.571410027397512 1105 26-27 0.6938059184725844 1105 28-29 0.5457738186249159 1105 30-31 0.46100002505996684 1105 32-33 0.39040302071683186 1105 34-35 0.44833400742958673 1105 36-37 0.6239474206231606 1105 38-39 0.6472693390999709 1105 40-41 0.426071923153593 1105 42-43 0.4419294158368885 1105 44-45 0.5096286708959852 1105 46-47 0.6853305710535551 1105 48-49 0.6183274897180056 1105 50-51 0.43180409241137596 1105 52-53 0.6320281345345293 1105 54-55 0.558998957492328 1105 56-57 0.5522176249997557 1105 58-59 0.5724119094622679 1105 60-61 0.6937689593484251 1105 62-63 0.7344756328819813 1105 64-65 0.5661966572488595 1105 66-67 0.5997959695845623 1105 68-69 0.6329345060887377 1105 70-71 0.5060324344578859 1105 72-73 0.7326251532902539 1105 74-75 0.7986462912328847 1105 76-77 0.6555533031788947 1105 78-79 0.546211963480058 1105 80-81 0.5124903679782129 1105 82-83 0.42262115494773056 1105 84-85 0.4853351947240867 1105 86-87 0.589657088214782 1105 88-89 0.5418988239814553 1105 90-91 0.43561937818133245 1105 92-93 0.5854788184243525 1105 94-95 0.600616198465957 1105 96-97 0.783696599758926 1105 98-99 0.6725336482954702 1105 100-101 0.5758768627182071 1106 1 0.2609274531507495 1106 2 0.24581092624030987 1106 3 0.2413162256665231 1106 4 0.2209703854185605 1106 5 0.24449676540479715 1106 6 0.2834588108356826 1106 7 0.28913385646911394 1106 8 0.2705518760606509 1106 9 0.37437713154741203 1106 10-11 0.3822962268358978 1106 12-13 0.4865028264877651 1106 14-15 0.47289887039517 1106 16-17 0.5524938283064031 1106 18-19 0.4860130505071041 1106 20-21 0.6581744243511167 1106 22-23 0.6484436814397938 1106 24-25 0.5662519012348213 1106 26-27 0.641958511412092 1106 28-29 0.4687873221178762 1106 30-31 0.43327211567299884 1106 32-33 0.573898643139394 1106 34-35 0.5200366227014541 1106 36-37 0.4575550301059579 1106 38-39 0.6640495105605098 1106 40-41 0.6541753632052902 1106 42-43 0.37792116803868225 1106 44-45 0.21247702328771823 1106 46-47 0.21704957784865542 1106 48-49 -0.01815199702784298 1106 50-51 0.10185422098509633 1106 52-53 0.1298859619205146 1106 54-55 -0.053980592131061655 1106 56-57 0.029407714632768034 1106 58-59 0.09957608921397565 1106 60-61 0.22436755349344395 1106 62-63 0.15738126887742254 1106 64-65 -0.021089288104057857 1106 66-67 -0.04396755450621015 1106 68-69 -0.05269136389786411 1106 70-71 0.1418489968345682 1106 72-73 0.09043465170097065 1106 74-75 0.04578482128617978 1106 76-77 0.32532156693498493 1106 78-79 0.5334288519526567 1106 80-81 0.5122898103195119 1106 82-83 0.47581826084650913 1106 84-85 0.3664852682257589 1106 86-87 0.3040169081682116 1106 88-89 0.47181205298144135 1106 90-91 0.5940957037048413 1106 92-93 0.6791354507035123 1106 94-95 0.6577193002798651 1106 96-97 0.585129340308864 1106 98-99 0.7387268904780555 1106 100-101 0.5068774394569893 1107 1 0.11266532643057303 1107 2 0.1301976615340763 1107 3 0.15874174966801036 1107 4 0.18512412451561033 1107 5 0.16602251856320294 1107 6 0.2445171318713264 1107 7 0.1793005051391674 1107 8 0.16494772964017557 1107 9 0.15594319712708682 1107 10-11 0.4071795487713743 1107 12-13 0.43827639305725086 1107 14-15 0.445767471098236 1107 16-17 0.42902054128435907 1107 18-19 0.5043231082162478 1107 20-21 0.34743884264048575 1107 22-23 0.35124005572143346 1107 24-25 0.5221601904764412 1107 26-27 0.5280834199292812 1107 28-29 0.34805252026063016 1107 30-31 0.383904396804148 1107 32-33 0.5126246626477311 1107 34-35 0.5258510295929568 1107 36-37 0.481505527412466 1107 38-39 0.5113977090991781 1107 40-41 0.36885400362088916 1107 42-43 0.5613876763351229 1107 44-45 0.6690061958032842 1107 46-47 0.4374736381253257 1107 48-49 0.2771289901465721 1107 50-51 0.3159929629016105 1107 52-53 0.24080510912083497 1107 54-55 0.08359904289755349 1107 56-57 0.22400962947278202 1107 58-59 0.04880463424751014 1107 60-61 -0.011967015607559972 1107 62-63 0.07172337256192662 1107 64-65 0.19262353388540276 1107 66-67 0.12751170739255713 1107 68-69 0.2069503234649126 1107 70-71 0.3098984035461072 1107 72-73 0.12427878900316003 1107 74-75 0.3436453235117618 1107 76-77 0.20780665973753898 1107 78-79 0.13166788301481347 1107 80-81 0.3829811203532252 1107 82-83 0.33355650605641074 1107 84-85 0.3333399995362747 1107 86-87 0.2356882539552032 1107 88-89 0.385248326961932 1107 90-91 0.4719196727986521 1107 92-93 0.7044731872090466 1107 94-95 0.617003454858807 1107 96-97 0.6217584540354864 1107 98-99 0.7986031337443222 1107 100-101 0.6130004883237987 1108 1 0.013300488395479704 1108 2 0.11932667376115091 1108 3 0.11035415885600486 1108 4 0.12494942016805055 1108 5 0.09239552376200777 1108 6 0.08304787777591116 1108 7 0.16389065720490237 1108 8 0.10659135393394337 1108 9 0.2068703219680188 1108 10-11 0.21028212422643833 1108 12-13 0.22683233938175107 1108 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-1.5638605169518627 2316 52-53 -1.4707968943178997 2316 54-55 -1.4782683414188043 2316 56-57 -1.5188998420081212 2316 58-59 -1.4121432613942666 2316 60-61 -1.2417270514900487 2316 62-63 -1.4252714693308732 2316 64-65 -1.4959389240648022 2316 66-67 -1.4201297667795316 2316 68-69 -1.4526744055475724 2316 70-71 -1.3344613183646317 2316 72-73 -1.04667134929392 2316 74-75 -0.9824666597430785 2316 76-77 -1.2045952240929196 2316 78-79 -1.0518411249839872 2316 80-81 -1.007054014579868 2316 82-83 -1.0134719756659472 2316 84-85 -1.071877911804087 2316 86-87 -0.9829556871377179 2316 88-89 -1.2146619668259113 2316 90-91 -1.1187135035488076 2316 92-93 -1.287916735793118 2316 94-95 -1.2248893462176653 2316 96-97 -1.2102218105978402 2316 98-99 -1.373640269111327 2316 100-101 -1.1017487080580501 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3661.0 3 661.0 4 573.0 5 630.0 6 758.0 7 929.0 8 1127.0 9 1489.0 10 2009.0 11 3408.0 12 5945.0 13 10209.0 14 10256.0 15 12874.0 16 18560.0 17 30000.0 18 26703.0 19 10549.0 20 8862.0 21 8164.0 22 8170.0 23 8427.0 24 9048.0 25 9872.0 26 10989.0 27 12625.0 28 14060.0 29 16319.0 30 19488.0 31 22792.0 32 27474.0 33 34821.0 34 46586.0 35 72259.0 36 100978.0 37 181288.0 38 244705.0 39 59608.0 40 39.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.129407163105626 22.148172198583175 15.652086406883994 17.07033423142721 2 14.351021676343015 24.155912802048373 41.64987633410915 19.843189187499465 3 18.977695642660457 31.92932170012115 32.707374068520515 16.385608588697874 4 10.239944679158063 19.89182123032037 41.72034556580717 28.1478885247144 5 9.29603939852445 42.55891445620013 34.65703936915367 13.48800677612175 6 22.534448357529964 41.84314219399413 19.70869345767665 15.913715990799252 7 22.40215093682049 39.844892377395084 22.453954496208016 15.299002189576406 8 24.65108614118699 33.608909615778025 21.218489782620225 20.521514460414757 9 27.267097038126415 18.158556876173666 23.935143140966698 30.639202944733224 10-11 22.240304245161298 29.618637667668413 27.82752306957307 20.31353501759722 12-13 26.018728177213102 29.30018400509873 28.41728151643687 16.26380630125129 14-15 22.763623621775068 28.659312976918507 31.11562528854827 17.461438112758152 16-17 16.285307377417862 32.31267168980169 31.903646333922637 19.498374598857815 18-19 15.906050597246393 30.023463673923594 37.76931728048679 16.30116844834322 20-21 18.17204077012767 27.871805776431106 37.30091601130167 16.65523744213955 22-23 17.423368730454104 28.210641680760943 36.88687524776485 17.479114341020104 24-25 17.103028098227743 29.189652379287708 37.63228923013376 16.07503029235079 26-27 17.146271727885587 27.97724611484374 38.88865204688335 15.987830110387321 28-29 15.709417854287372 28.945057464295594 39.03110233684346 16.314422344573572 30-31 16.316584651962433 29.3180386566323 38.199449656864374 16.1659270345409 32-33 16.059769971234562 29.68153433335133 37.774595299593386 16.484100395820725 34-35 16.746683323421234 29.164825452938576 37.585156629342436 16.50333459429775 36-37 17.408780239208678 28.552107207018334 37.765097439100856 16.274015114672128 38-39 16.840323737399903 29.685449639766336 37.35570097873103 16.118525644102732 40-41 16.19608184141764 29.875947311225566 38.05695295197122 15.871017895385576 42-43 15.759653625002198 30.575702510686376 36.703049223636505 16.961594640674914 44-45 16.60444213736742 30.38948086964127 35.78671009549017 17.219366897501132 46-47 17.315625888390993 30.877071882300257 34.35923180266239 17.44807042664636 48-49 17.334143491114414 30.90395351291831 34.106976960900376 17.6549260350669 50-51 17.185191382874137 31.56567145163468 33.903453810068015 17.345683355423162 52-53 16.87879315148075 32.507409213953 33.49853717347877 17.115260461087484 54-55 16.446599745027225 31.604075381437184 33.7820866888429 18.167238184692696 56-57 17.1845126668273 32.36327207005808 32.72274411568879 17.729471147425834 58-59 17.16862918760091 31.325435734764607 33.267176849253495 18.238758228380988 60-61 16.981172402656004 31.524873896894682 33.752647927729384 17.741305772719933 62-63 17.34577964514703 31.13770633611696 33.682989776470194 17.83352424226581 64-65 16.99061483749501 31.69503451685906 33.66002320141491 17.654327444231026 66-67 16.67592261519852 31.50509330756883 32.99186173895182 18.827122338280827 68-69 16.292419085009836 31.84114592992678 33.219061803635576 18.64737318142781 70-71 16.879184418746195 32.061444385270846 32.69457547169811 18.364795724284843 72-73 17.204608333931617 30.934423337785212 32.92217267416401 18.938795654119158 74-75 16.677246412990502 32.43341297861465 32.28881518728529 18.600525421109566 76-77 17.08155481681458 31.31130325982528 32.75855102185371 18.84859090150643 78-79 16.802019382996935 31.237318851772972 32.99971625596659 18.960945509263503 80-81 16.995083139851282 31.720093061499966 32.25037250740856 19.034451291240195 82-83 16.826819551470514 31.87322071056068 32.17967798931321 19.120281748655596 84-85 16.807559941437074 31.343810012739336 31.888274104918906 19.960355940904684 86-87 17.01857407501601 31.264608669486236 32.24952475030079 19.46729250519697 88-89 17.845314899423308 30.51284367467445 32.008015708125534 19.63382571777671 90-91 17.25033843912399 30.294664335434895 32.810692346354344 19.644304879086768 92-93 17.78760089977135 31.019431808942656 32.2507454113991 18.942221879886898 94-95 17.800887545688443 30.44862757553957 31.9672407865901 19.783244092181885 96-97 17.98619851486396 29.85573249482789 31.587931569815513 20.57013742049263 98-99 18.20405671469916 29.99920598280073 31.04740377771512 20.749333524784987 100-101 18.83278734037966 29.212500837886445 32.06492776111513 19.88978406061877 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5674.0 1 3422.0 2 1481.5 3 1732.0 4 1420.5 5 1379.0 6 2300.0 7 4460.0 8 6807.5 9 7410.0 10 6130.0 11 5033.5 12 5128.5 13 5491.5 14 5759.0 15 6018.5 16 6207.5 17 6476.0 18 6965.5 19 7379.0 20 7890.0 21 8637.0 22 9528.0 23 10511.5 24 11471.5 25 13401.5 26 17135.5 27 20695.0 28 21323.5 29 21971.0 30 24874.5 31 27825.5 32 31624.5 33 36030.5 34 41925.5 35 45292.0 36 46314.0 37 49621.0 38 47072.0 39 41210.0 40 37342.5 41 34153.0 42 31396.5 43 29742.0 44 28869.5 45 27043.5 46 25910.5 47 28584.0 48 49489.5 49 48307.5 50 24363.5 51 15549.0 52 9974.5 53 7403.0 54 5901.0 55 4771.0 56 3885.0 57 3204.0 58 2582.5 59 2090.5 60 1739.0 61 1511.5 62 1260.0 63 851.0 64 517.5 65 390.0 66 326.5 67 270.0 68 208.0 69 170.5 70 142.5 71 121.0 72 108.5 73 116.5 74 97.5 75 69.5 76 53.5 77 42.0 78 36.0 79 28.0 80 21.5 81 19.0 82 19.5 83 12.5 84 6.0 85 3.5 86 3.0 87 1.5 88 2.0 89 2.0 90 1.0 91 1.5 92 1.0 93 0.0 94 1.0 95 1.0 96 1.0 97 2.0 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3380593519819475 2 0.11807950497438298 3 0.11372721552821183 4 0.11874180989010469 5 0.13662404261459057 6 0.0864780989956619 7 0.0948988329241235 8 0.10095419215357905 9 0.0878027088271053 10-11 0.08747155636924445 12-13 0.09139807836959453 14-15 0.09295922567093853 16-17 0.08212580954949074 18-19 0.09721690012914946 20-21 0.10805031625059727 22-23 0.11604528273323778 24-25 0.12943330352961213 26-27 0.12858176863796994 28-29 0.12328332931219634 30-31 0.11448413543189376 32-33 0.1072933963469153 34-35 0.33006438549930694 36-37 0.48778757042903165 38-39 0.4626199836316071 40-41 0.4409531513887115 42-43 0.47227071240355184 44-45 0.4805495238500731 46-47 0.4876456479470913 48-49 0.49843175657455896 50-51 0.4871725730072901 52-53 0.4904840975858986 54-55 0.47667030934370314 56-57 0.4557130895105094 58-59 0.43636432447263973 60-61 0.3978560243728209 62-63 0.46645189064399695 64-65 0.49720176173107583 66-67 0.5051494207197362 68-69 0.5177332141184485 70-71 0.5104478600455098 72-73 0.5013175137073463 74-75 0.5087920977562056 76-77 0.4849491207902244 78-79 0.4646068983787722 80-81 0.46574227823429515 82-83 0.47832607163300733 84-85 0.47827876413902726 86-87 0.48248913110325803 88-89 0.4948363870320697 90-91 0.5105897825274501 92-93 0.5028313535147102 94-95 0.5074674879247622 96-97 0.5072782579488417 98-99 0.5015067436832669 100-101 0.4883552603567931 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1056915.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.2150296477697 #Duplication Level Percentage of deduplicated Percentage of total 1 77.20480883961886 25.643600145583324 2 11.469757611591348 7.619366782434773 3 4.043157694516175 4.028808080818887 4 1.9147448971678833 2.5439323410938792 5 1.1041729837814072 1.8337569196282884 6 0.6945367696653022 1.384143563753952 7 0.4583824973976975 1.0657631768758264 8 0.3465323684351867 0.9208066313189261 9 0.2658653199798101 0.7947652036898853 >10 1.8977891262773505 13.00744805944005 >50 0.324090491906623 7.617048749589278 >100 0.24107064330036176 15.768152230256941 >500 0.021682093361880643 4.952315307849498 >1k 0.011411628085200338 6.548063457254143 >5k 0.0014264535106500423 3.196329027765325 >10k+ 5.705814042600169E-4 3.0757003226470285 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18379 1.738928863721302 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14079 1.3320844154922582 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9710 0.9187115330939574 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6836 0.6467880576962196 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5997 0.567406082797576 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5733 0.5424277259760719 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5455 0.5161247593231244 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4232 0.4004106290477475 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3577 0.3384378119337884 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3091 0.29245492778511045 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2958 0.2798711343863981 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2709 0.2563120023842977 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2697 0.25517662252877477 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2477 0.23436132517752137 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2439 0.23076595563503213 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2377 0.2248998263814971 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2049 0.19386611033053747 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2040 0.19301457543889528 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1788 0.1691715984729141 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1705 0.16131855447221394 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1690 0.1598993296528103 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1597 0.15110013577250772 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1530 0.14476093157917144 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1522 0.1440040116754895 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1472 0.13927326227747738 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1471 0.13917864728951712 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1460 0.13813788242195446 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1452 0.13738096251827253 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1381 0.1306632983730953 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1374 0.1300009934573736 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1370 0.12962253350553263 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1351 0.127824848734288 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1344 0.12716254381856631 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1320 0.12489178410752047 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1268 0.11997180473358784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1254 0.11864719490214445 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1214 0.11486259538373474 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1194 0.11297029562452988 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1163 0.11003723099776234 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1158 0.10956415605796115 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1116 0.10559032656363093 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1081 0.10227880198502246 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 1074 0.10161649706930075 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 1060 0.10029188723785734 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.8922997592048555E-4 0.0 14-15 0.0 0.0 0.0 2.8384496388072833E-4 0.0 16-17 0.0 0.0 0.0 3.3115245786084974E-4 0.0 18-19 0.0 0.0 0.0 4.7307493980121393E-4 0.0 20-21 0.0 0.0 0.0 8.51534891642185E-4 0.0 22-23 0.0 0.0 0.0 0.002649219662886798 0.0 24-25 0.0 0.0 0.0 0.006197281711395902 0.0 26-27 0.0 0.0 0.0 0.01026572619368634 0.0 28-29 0.0 0.0 0.0 0.022187214676676933 0.0 30-31 0.0 0.0 0.0 0.055444382944702275 0.0 32-33 0.0 0.0 0.0 0.11174030078104671 0.0 34-35 0.0 0.0 0.0 0.19362957286063687 0.0 36-37 0.0 0.0 0.0 0.3165817497149723 0.0 38-39 0.0 0.0 0.0 0.4956879219237119 0.0 40-41 0.0 4.730749398012139E-5 0.0 0.7040301254121666 0.0 42-43 0.0 9.461498796024278E-5 0.0 0.9035731350203186 0.0 44-45 0.0 9.461498796024278E-5 0.0 1.1248775918593266 0.0 46-47 0.0 9.461498796024278E-5 0.0 1.39117147547343 0.0 48-49 0.0 9.461498796024278E-5 0.0 1.6834844807765998 0.0 50-51 0.0 9.461498796024278E-5 0.0 2.010237341697298 0.0 52-53 0.0 9.461498796024278E-5 0.0 2.3058618715790766 0.0 54-55 0.0 9.461498796024278E-5 0.0 2.6139755798716076 0.0 56-57 0.0 9.461498796024278E-5 0.0 2.9904013094714332 0.0 58-59 0.0 9.461498796024278E-5 0.0 3.416310677774466 0.0 60-61 0.0 9.461498796024278E-5 0.0 3.9115728322523573 0.0 62-63 0.0 9.461498796024278E-5 0.0 4.379538562703718 0.0 64-65 0.0 9.461498796024278E-5 0.0 4.838468561804875 0.0 66-67 0.0 9.461498796024278E-5 0.0 5.352511791392875 0.0 68-69 0.0 9.461498796024278E-5 0.0 5.925358235998164 0.0 70-71 0.0 9.461498796024278E-5 0.0 6.573376288537867 0.0 72-73 0.0 9.461498796024278E-5 0.0 7.226692780403344 0.0 74-75 0.0 9.461498796024278E-5 0.0 7.874426987979166 0.0 76-77 0.0 9.461498796024278E-5 0.0 8.53881343343599 0.0 78-79 0.0 9.461498796024278E-5 0.0 9.295023724708232 0.0 80-81 0.0 9.461498796024278E-5 0.0 10.117038740106821 0.0 82-83 0.0 9.461498796024278E-5 0.0 10.930869559046847 0.0 84-85 0.0 9.461498796024278E-5 0.0 11.782735603146895 0.0 86-87 0.0 9.461498796024278E-5 0.0 12.663601141056755 0.0 88-89 0.0 9.461498796024278E-5 0.0 13.541486306845869 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 365 0.0 35.03212 9 TAGGCAT 385 0.0 34.45867 5 GTACACG 70 2.0578153E-4 33.91565 1 GGGATCG 70 2.0884236E-4 33.830517 7 CGTGCAA 115 1.06381776E-7 32.94486 9 ATGGGCG 195 0.0 31.587118 5 TGGGCGT 90 2.4609793E-5 31.57515 6 ACCGTGC 120 1.5443402E-7 31.575148 7 CCTTTAG 410 0.0 31.268576 1 GGCATTC 450 0.0 30.522642 7 CCGTGCA 130 3.114401E-7 29.146292 8 ATGGGTA 550 0.0 28.428404 5 TTAGGCA 470 0.0 28.226786 4 TGACCGT 135 4.3185173E-7 28.077436 5 TTCGATA 85 6.501751E-4 27.870983 4 GTCAGGC 155 4.7000867E-8 27.511358 2 CATGGGT 1435 0.0 27.40485 4 GATCGCG 35 0.008348537 27.172516 92-93 CCGGATC 35 0.008348537 27.172516 92-93 TCGCGGG 35 0.008348537 27.172516 94-95 >>END_MODULE