##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939120_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1040689 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22995534689038 33.0 31.0 34.0 31.0 34.0 2 32.278716311981775 34.0 31.0 34.0 31.0 34.0 3 32.28176909720387 34.0 31.0 34.0 30.0 34.0 4 35.76948733002847 37.0 35.0 37.0 35.0 37.0 5 35.85164251760132 37.0 35.0 37.0 35.0 37.0 6 35.98970201472294 37.0 37.0 37.0 35.0 37.0 7 35.95080566816792 37.0 37.0 37.0 35.0 37.0 8 35.96867363832999 37.0 37.0 37.0 35.0 37.0 9 37.61501370726509 39.0 38.0 39.0 35.0 39.0 10-11 37.548712919998195 39.0 38.0 39.0 35.0 39.0 12-13 37.47300202077662 39.0 38.0 39.0 35.0 39.0 14-15 38.63589554612377 40.0 38.0 41.0 34.5 41.0 16-17 38.60650780396449 40.0 38.0 41.0 34.5 41.0 18-19 38.71338843785223 40.0 38.5 41.0 35.0 41.0 20-21 38.7671595452628 40.5 38.5 41.0 35.0 41.0 22-23 38.77109780155263 41.0 39.0 41.0 35.0 41.0 24-25 38.702022890604205 41.0 38.0 41.0 35.0 41.0 26-27 38.53761978842863 40.0 38.0 41.0 35.0 41.0 28-29 38.371879591309224 40.0 38.0 41.0 34.5 41.0 30-31 38.013685644798784 40.0 37.5 41.0 34.0 41.0 32-33 37.61114655771321 40.0 35.5 41.0 33.5 41.0 34-35 37.22059183867611 40.0 35.0 41.0 33.0 41.0 36-37 36.85556299720666 40.0 35.0 41.0 32.5 41.0 38-39 36.577283415122096 40.0 35.0 41.0 31.5 41.0 40-41 36.30459964504285 40.0 35.0 41.0 31.0 41.0 42-43 35.91492847526975 39.0 35.0 41.0 30.0 41.0 44-45 35.29102258215471 39.0 35.0 41.0 25.5 41.0 46-47 34.42605523840456 39.0 35.0 41.0 12.5 41.0 48-49 33.87866259756757 39.5 35.0 41.0 2.0 41.0 50-51 32.67574222462234 38.0 34.0 40.0 2.0 40.5 52-53 32.76687223560545 38.5 33.5 39.5 2.0 40.5 54-55 33.254522244397705 39.0 35.0 41.0 2.0 41.0 56-57 33.18435478802985 39.0 35.0 41.0 2.0 41.0 58-59 33.00958163293741 39.0 34.5 41.0 2.0 41.0 60-61 32.70379335228873 39.0 34.0 41.0 2.0 41.0 62-63 32.33499537325753 38.0 33.5 40.5 2.0 41.0 64-65 31.97011306932234 38.0 33.0 40.0 2.0 41.0 66-67 31.57575077664893 37.0 32.5 40.0 2.0 41.0 68-69 31.155599799748053 37.0 32.0 39.5 2.0 41.0 70-71 30.705780016892653 36.0 31.5 39.0 2.0 41.0 72-73 30.216053499172183 35.5 31.0 39.0 2.0 40.5 74-75 29.729890966465486 35.0 30.5 37.5 2.0 40.0 76-77 29.244325634267298 35.0 29.5 37.0 2.0 39.5 78-79 28.76802435694045 35.0 29.0 36.5 2.0 39.0 80-81 28.260948756064494 35.0 28.5 36.0 2.0 39.0 82-83 27.80532704775394 35.0 27.5 36.0 2.0 37.5 84-85 27.358059900700404 34.5 26.0 35.0 2.0 37.0 86-87 26.905000917661283 34.0 25.5 35.0 2.0 36.5 88-89 26.380589205805002 34.0 23.0 35.0 2.0 36.0 90-91 25.89119179697297 34.0 19.0 35.0 2.0 36.0 92-93 25.453020066513627 34.0 10.5 35.0 2.0 35.5 94-95 24.94589930325006 34.0 2.0 35.0 2.0 35.0 96-97 24.33587796161966 33.0 2.0 35.0 2.0 35.0 98-99 23.699343896207225 33.0 2.0 35.0 2.0 35.0 100-101 21.201411276567733 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2100504946038555 1101 2 0.43941242706996775 1101 3 0.4281038862944868 1101 4 0.4910519166572982 1101 5 0.13815718359703766 1101 6 0.27467174274482886 1101 7 0.36251822890836394 1101 8 0.34539566788187415 1101 9 0.2879551502153177 1101 10-11 0.47376736327416324 1101 12-13 0.5777352883225291 1101 14-15 0.7303797376686632 1101 16-17 0.7102994899844717 1101 18-19 0.750319061219713 1101 20-21 0.8102993870002351 1101 22-23 0.7223723135768623 1101 24-25 0.6899276979410089 1101 26-27 0.6080555540085371 1101 28-29 0.636398501252124 1101 30-31 0.6504330746223488 1101 32-33 0.5771543430095889 1101 34-35 0.6949593974150474 1101 36-37 0.8955632761016759 1101 38-39 0.7211984244137497 1101 40-41 0.6908872968673236 1101 42-43 0.7704243530652519 1101 44-45 0.6064357035757908 1101 46-47 1.0147490533007257 1101 48-49 0.8603512647002205 1101 50-51 0.9528985762104796 1101 52-53 0.8957508521391375 1101 54-55 0.7625149529539854 1101 56-57 0.7150476309103837 1101 58-59 0.6623321662751636 1101 60-61 0.7080363050532625 1101 62-63 0.6955937943446386 1101 64-65 0.4913757361973268 1101 66-67 0.4775554177928001 1101 68-69 0.623098312372413 1101 70-71 0.6374180510657474 1101 72-73 0.8055963777574142 1101 74-75 0.5633615095084821 1101 76-77 0.40140232668988673 1101 78-79 0.4741948454818967 1101 80-81 0.2711626314014026 1101 82-83 0.3257544319568062 1101 84-85 0.4007021543701548 1101 86-87 0.30698308631504645 1101 88-89 0.22211690618448188 1101 90-91 0.334439006532417 1101 92-93 0.4031510082602807 1101 94-95 0.29955540981509543 1101 96-97 0.496725888373998 1101 98-99 0.5584351789148023 1101 100-101 0.222540048285083 1102 1 0.3649834158879699 1102 2 0.3853269429523678 1102 3 0.36284398141991403 1102 4 0.287888312192365 1102 5 0.3197525866729194 1102 6 0.27644629065762416 1102 7 0.3161050154341396 1102 8 0.419231883681924 1102 9 0.49536398834918316 1102 10-11 0.520017608062517 1102 12-13 0.4966077327991627 1102 14-15 0.6282433053818153 1102 16-17 0.4449469008613889 1102 18-19 0.464713421296004 1102 20-21 0.36508838614447825 1102 22-23 0.5033692258903244 1102 24-25 0.5609490488508158 1102 26-27 0.5036214644468586 1102 28-29 0.5982906548799534 1102 30-31 0.44403826386722045 1102 32-33 0.41763415501213075 1102 34-35 0.32985857143877695 1102 36-37 0.341781597805884 1102 38-39 0.3305691909165063 1102 40-41 0.2141254242967605 1102 42-43 0.23253983415149548 1102 44-45 0.4196722482640851 1102 46-47 0.5925890676795262 1102 48-49 0.38761733421743116 1102 50-51 0.18019294728548374 1102 52-53 0.216439022624499 1102 54-55 0.271841053965062 1102 56-57 0.3438881254767594 1102 58-59 0.31723155490786326 1102 60-61 0.2864369853637001 1102 62-63 0.16942290122259607 1102 64-65 0.31499667237530105 1102 66-67 0.40781756264848923 1102 68-69 0.3462654847114628 1102 70-71 0.3161517776700187 1102 72-73 0.359443109396949 1102 74-75 0.059737300543726946 1102 76-77 0.014200130167086655 1102 78-79 0.0301112123666627 1102 80-81 -0.12290906408993152 1102 82-83 -0.31987854378433056 1102 84-85 -0.31763363355326035 1102 86-87 -0.2666143770069347 1102 88-89 -0.4299047103039264 1102 90-91 -0.21179514258325938 1102 92-93 -0.218382721563124 1102 94-95 -0.15877088166144304 1102 96-97 -0.2686796823383162 1102 98-99 -0.26784422586721846 1102 100-101 -0.5489007290286025 1103 1 0.2768436231782445 1103 2 0.3582182358928989 1103 3 0.4344171228499576 1103 4 0.42699058039141136 1103 5 0.43159227798637545 1103 6 0.3643651420785545 1103 7 0.4293006023949175 1103 8 0.43282108806970854 1103 9 0.43141726676282843 1103 10-11 0.407745240058496 1103 12-13 0.3617395150720384 1103 14-15 0.5262401043633815 1103 16-17 0.5462353305080185 1103 18-19 0.585223085504694 1103 20-21 0.5508954070799845 1103 22-23 0.6326856361730364 1103 24-25 0.7246625984698909 1103 26-27 0.6639860438759015 1103 28-29 0.7275182941091245 1103 30-31 0.7391329727178899 1103 32-33 0.8127915954072975 1103 34-35 0.8754862353923372 1103 36-37 0.8785426774197092 1103 38-39 0.7868925174506316 1103 40-41 0.8136969560117038 1103 42-43 0.5942055999563678 1103 44-45 0.7814761023744481 1103 46-47 0.7122971621529715 1103 48-49 0.8333801931977121 1103 50-51 0.709970046457876 1103 52-53 0.5891965671777086 1103 54-55 0.7227789365847457 1103 56-57 0.7316723079216558 1103 58-59 0.6460086317230989 1103 60-61 0.6741698778665537 1103 62-63 0.6593135221333668 1103 64-65 0.578200215852732 1103 66-67 0.6014614237027232 1103 68-69 0.6748551559692366 1103 70-71 0.6602947950704596 1103 72-73 0.7347243821239822 1103 74-75 0.8338439020853201 1103 76-77 0.7871073361409167 1103 78-79 0.6911478215491371 1103 80-81 0.7616201797253588 1103 82-83 0.7697789051591606 1103 84-85 0.6762885083147232 1103 86-87 0.7080188012729565 1103 88-89 0.8634757308684833 1103 90-91 1.028187208416167 1103 92-93 1.136568007214347 1103 94-95 1.1360397598179155 1103 96-97 1.1100630039132184 1103 98-99 0.9004913366058993 1103 100-101 0.704898343281009 1104 1 0.2902561540743136 1104 2 0.3549243258662784 1104 3 0.42418193667134574 1104 4 0.2634356643435041 1104 5 0.30789016376741785 1104 6 0.3283978505549854 1104 7 0.40916488528011996 1104 8 0.38868795795274735 1104 9 0.46752368462313854 1104 10-11 0.5265334371315831 1104 12-13 0.47134537392022935 1104 14-15 0.5249823270758185 1104 16-17 0.46702356267604017 1104 18-19 0.5367939597194464 1104 20-21 0.5476591530127308 1104 22-23 0.5960070455854947 1104 24-25 0.5906632552464686 1104 26-27 0.5911571667633169 1104 28-29 0.6389769146777624 1104 30-31 0.5405545681334942 1104 32-33 0.3675077275447336 1104 34-35 0.4786615746851268 1104 36-37 0.278292124674536 1104 38-39 0.17108362940847854 1104 40-41 0.2071644104760324 1104 42-43 0.34718616237771016 1104 44-45 0.3338972766508661 1104 46-47 0.3478143611763329 1104 48-49 0.17983547974782965 1104 50-51 0.3181614542158613 1104 52-53 0.3510523871707889 1104 54-55 0.07016173204645781 1104 56-57 0.02664959648722487 1104 58-59 0.041473990518639425 1104 60-61 0.16347845757466217 1104 62-63 0.06441557318145641 1104 64-65 0.27391462332494854 1104 66-67 0.2178374709104176 1104 68-69 0.040769328806764094 1104 70-71 0.08215154257245061 1104 72-73 0.05217809441572996 1104 74-75 0.1786704077832333 1104 76-77 -0.06014134654575187 1104 78-79 0.08644083916178147 1104 80-81 0.01122097487624174 1104 82-83 -0.12606873771481375 1104 84-85 -0.15107576203658013 1104 86-87 -0.16805347423228412 1104 88-89 0.007061630727601909 1104 90-91 -0.12039721244225277 1104 92-93 -0.12038580284185585 1104 94-95 -0.11212402321519122 1104 96-97 0.06726473459471194 1104 98-99 -0.02448823313023496 1104 100-101 0.03514039803913249 1105 1 0.10165467191850297 1105 2 0.2514294536702124 1105 3 0.262773461926308 1105 4 0.17519144527272346 1105 5 0.27969079816423914 1105 6 0.28854654027424687 1105 7 0.26589812094100296 1105 8 0.3236089428324789 1105 9 0.437538003594824 1105 10-11 0.3169140041059677 1105 12-13 0.3210216561082717 1105 14-15 0.3017231252399526 1105 16-17 0.36532330332247653 1105 18-19 0.3500185020576154 1105 20-21 0.4952621476691945 1105 22-23 0.4531735249675961 1105 24-25 0.451537494847436 1105 26-27 0.4300479917162576 1105 28-29 0.22817711476405833 1105 30-31 0.44169321086566526 1105 32-33 0.297252902003855 1105 34-35 0.3550478360585174 1105 36-37 0.3179448896110699 1105 38-39 0.12591900864786965 1105 40-41 0.3960545242217677 1105 42-43 0.1611379140810527 1105 44-45 0.21158563987861356 1105 46-47 0.11335127520085564 1105 48-49 -0.17288951065086877 1105 50-51 -0.16558390515714194 1105 52-53 -0.12734350868950628 1105 54-55 0.059674389163987485 1105 56-57 -0.02065101657338886 1105 58-59 0.05256704486909314 1105 60-61 -0.10733976260560496 1105 62-63 -0.008832842011827324 1105 64-65 0.07177531205800847 1105 66-67 0.25534061207556746 1105 68-69 -0.004807792131650501 1105 70-71 -0.03343088871307742 1105 72-73 0.04035546000942247 1105 74-75 0.03267497847485501 1105 76-77 -0.0529575611217048 1105 78-79 -0.07500201059806955 1105 80-81 0.12497441830445055 1105 82-83 0.05178344023871162 1105 84-85 0.13844839293072653 1105 86-87 0.14952899024291355 1105 88-89 -0.16152766607812907 1105 90-91 -0.08540107666805596 1105 92-93 -0.12868677624744507 1105 94-95 0.05271484093949752 1105 96-97 0.06602486337062885 1105 98-99 -0.0375365146165656 1105 100-101 0.08636983822423616 1106 1 0.06898996010070135 1106 2 0.14127281745494713 1106 3 0.23743943333495565 1106 4 0.1787049813595445 1106 5 0.20192460802019951 1106 6 0.15618566524236144 1106 7 0.1285745031613601 1106 8 0.17473004639658285 1106 9 0.3514377464847982 1106 10-11 0.23583969501862612 1106 12-13 0.23327580451877594 1106 14-15 0.2704918187643486 1106 16-17 0.46221416812864646 1106 18-19 0.3185134941682435 1106 20-21 0.3182721408746261 1106 22-23 0.28014739184374093 1106 24-25 0.29020546625156385 1106 26-27 0.16536687361494273 1106 28-29 0.21102591194510723 1106 30-31 0.24110337021067352 1106 32-33 0.11191189825894554 1106 34-35 0.06659927725164749 1106 36-37 0.022560116213270476 1106 38-39 0.020679404222597952 1106 40-41 0.2129894747072072 1106 42-43 -0.07383672480237635 1106 44-45 -0.3631619340546308 1106 46-47 -0.37656466178347614 1106 48-49 -0.18545494504041216 1106 50-51 -0.1640331185900692 1106 52-53 -0.15793316875799945 1106 54-55 -0.18229289759157297 1106 56-57 -0.47427301742665406 1106 58-59 -0.4031271860694403 1106 60-61 -0.3165599449528216 1106 62-63 -0.5022668297454871 1106 64-65 -0.40006335196306964 1106 66-67 -0.5722128245983917 1106 68-69 -0.6266777201905036 1106 70-71 -0.6231011151911225 1106 72-73 -0.516819354494384 1106 74-75 -0.5969517040834198 1106 76-77 -0.5026884872369948 1106 78-79 -0.6766959119662381 1106 80-81 -0.6467253069783361 1106 82-83 -0.5081092010871551 1106 84-85 -0.3692711156031159 1106 86-87 -0.3098001083369972 1106 88-89 -0.2578976516989293 1106 90-91 -0.10508613381239584 1106 92-93 0.059722504995711745 1106 94-95 -0.041008988126353785 1106 96-97 -0.04375987752160171 1106 98-99 -0.19232679725700663 1106 100-101 -0.32163996310804066 1107 1 0.1718814895977303 1107 2 0.2212402231104278 1107 3 0.1198560772194881 1107 4 0.2027343035540028 1107 5 0.22467813077372512 1107 6 0.2691320625924263 1107 7 0.13767358106777294 1107 8 0.12651563579684932 1107 9 0.11072596907577292 1107 10-11 0.07569401652432362 1107 12-13 0.0800855641725775 1107 14-15 0.09676375054086606 1107 16-17 0.028025392196759924 1107 18-19 0.08749471957148103 1107 20-21 0.11967816934487985 1107 22-23 0.09534879649469019 1107 24-25 0.10722520299607652 1107 26-27 0.11617230148458901 1107 28-29 0.06965377057296251 1107 30-31 0.20093476123472698 1107 32-33 3.7110552549535214E-4 1107 34-35 -0.062140966167497425 1107 36-37 0.1266739336298386 1107 38-39 0.18815192261254765 1107 40-41 0.39481102808839097 1107 42-43 0.34967782715254714 1107 44-45 0.6163261991032272 1107 46-47 0.48978528025453727 1107 48-49 0.2693601382150419 1107 50-51 0.41308175210279074 1107 52-53 0.2961756683783676 1107 54-55 0.356537923349336 1107 56-57 0.355059137593571 1107 58-59 0.4444476293952846 1107 60-61 0.46673375806565076 1107 62-63 0.381435680103813 1107 64-65 0.28032239723002306 1107 66-67 0.32285796955497403 1107 68-69 0.29235457957573985 1107 70-71 0.42275454993059824 1107 72-73 0.33703803147309586 1107 74-75 0.3167914321367782 1107 76-77 0.26810683172346117 1107 78-79 0.43714905508974056 1107 80-81 0.5382036233634366 1107 82-83 0.5537723699687298 1107 84-85 0.5966028388029514 1107 86-87 0.47660997853180476 1107 88-89 0.49328013158894635 1107 90-91 0.7990816189792085 1107 92-93 0.6167318785188449 1107 94-95 0.6871784441895166 1107 96-97 0.44662765185675113 1107 98-99 0.5573394180055367 1107 100-101 0.6695645441515126 1108 1 -0.022225212439778375 1108 2 0.08273513241123709 1108 3 0.07426699305408135 1108 4 0.16065774042073855 1108 5 0.16577585500397873 1108 6 0.16850938840945417 1108 7 0.0927200667045085 1108 8 0.13868357360311734 1108 9 0.13030581752154546 1108 10-11 0.06523628345135535 1108 12-13 0.1584233802687578 1108 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-0.9711625864881555 2316 98-99 -1.2279099469004144 2316 100-101 -1.1501327313425591 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3384.0 3 639.0 4 531.0 5 558.0 6 727.0 7 883.0 8 1136.0 9 1476.0 10 2044.0 11 3329.0 12 5883.0 13 10522.0 14 9840.0 15 12706.0 16 18440.0 17 32094.0 18 23600.0 19 10768.0 20 8792.0 21 7973.0 22 7946.0 23 8200.0 24 8882.0 25 9556.0 26 11084.0 27 12204.0 28 14127.0 29 15916.0 30 19108.0 31 22444.0 32 27236.0 33 34438.0 34 46309.0 35 71912.0 36 100742.0 37 180566.0 38 239842.0 39 54826.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.215539030875085 22.07244318729958 15.654797967129863 17.057219814695472 2 14.327772609063349 24.175344173843268 41.72890771647907 19.767975500614313 3 18.969329261604713 32.05408702646431 32.5722895391596 16.404294172771372 4 10.222827797038777 19.749950203659814 41.80785252200421 28.219369477297203 5 9.292763474425247 42.44131220482897 34.747139222576756 13.518785098169026 6 22.64740812686018 41.846646504180754 19.661501031364047 15.844444337595023 7 22.398710511333036 39.72457953129087 22.531429362795635 15.345280594580455 8 24.69614991796673 33.532181469520744 21.218150798886732 20.553517813625792 9 27.25570543958163 18.17470170920362 23.95548934885237 30.614103502362383 10-11 22.258546905803723 29.608693561571382 27.788671442996588 20.34408808962831 12-13 26.004218062076482 29.298653413014254 28.391375765281097 16.305752759628163 14-15 22.74513556281395 28.64285243759187 31.108885402336927 17.50312659725725 16-17 16.316529203392594 32.275508040315295 31.827079487639367 19.580883268652748 18-19 15.880674099489545 30.073166098825926 37.74827303481821 16.297886766866313 20-21 18.262731588342103 27.809691702828303 37.248647785676916 16.67892892315268 22-23 17.410856843862895 28.229948560897817 36.85855016370485 17.50064443153444 24-25 17.134468692176085 29.102584883761757 37.64653935525329 16.116407068808865 26-27 17.148928678965397 28.006966737244728 38.867265473862226 15.97683910992765 28-29 15.77462715855573 28.98058261766245 38.89002716471204 16.354763059069782 30-31 16.310219706496934 29.27008966679732 38.204788481098795 16.214902145606953 32-33 16.065566675162664 29.62648058824378 37.78552570200201 16.522427034591544 34-35 16.778098649606356 29.12786072769874 37.597205683127555 16.496834939567353 36-37 17.429472778132276 28.60007274557549 37.6803242539835 16.29013022230874 38-39 16.830214265948246 29.68047246976917 37.32487995321278 16.164433311069804 40-41 16.236140092368807 29.868505288080407 37.98413720717128 15.911217412379505 42-43 15.829227513452377 30.55352987823077 36.64609651711211 16.97114609120474 44-45 16.554451868545435 30.42714715851846 35.74767415913765 17.270726813798458 46-47 17.33175429157083 30.928144262079716 34.24268300575623 17.497418440593222 48-49 17.4053832127806 30.944416939369425 34.03910014128216 17.61109970656781 50-51 17.235832989589138 31.560112406766432 33.84114091515492 17.362913688489513 52-53 16.86406265924063 32.54355616113618 33.429966484300174 17.162414695323015 54-55 16.476972518094435 31.624427802403833 33.684580633133805 18.214019046367927 56-57 17.230386097999983 32.30847669738662 32.68874228460629 17.772394920007105 58-59 17.206769933677514 31.299356858231704 33.17813993861139 18.315733269479395 60-61 16.99115556649248 31.63286674483351 33.629161057471926 17.74681663120208 62-63 17.413526093681416 31.160304554653766 33.581607734494476 17.84456161717034 64-65 16.945415200469505 31.715939746844 33.61651769217951 17.72212736050699 66-67 16.660080578931606 31.594429039332955 32.831276992492825 18.914213389242615 68-69 16.311716122024777 31.815722059180217 33.09925505320152 18.77330676559349 70-71 16.9406117153239 32.03004707174878 32.617586368663154 18.41175484426416 72-73 17.225292959975423 30.90125106396957 32.81108248094999 19.062373495105014 74-75 16.69657759409865 32.49780800807716 32.16940776363685 18.636206634187342 76-77 17.14087422520287 31.271328956379534 32.65478314373724 18.93301367468035 78-79 16.796048159077916 31.254248875979172 32.87683667320736 19.07286629173555 80-81 17.02224745989576 31.80188297509679 32.171853105151285 19.004016459856167 82-83 16.855984233221598 31.82336456135488 32.17189690551378 19.14875429990974 84-85 16.857163138992966 31.264665105608074 31.933434967937774 19.94473678746118 86-87 17.01382332701525 31.238159626226896 32.287187164225955 19.4608298825319 88-89 17.8349055601384 30.52367420034735 32.00513493202687 19.63628530748738 90-91 17.29495613293542 30.290350598371685 32.67195396590919 19.742739302783708 92-93 17.814222063768806 31.06208716966931 32.178990526128146 18.944700240433736 94-95 17.821314895674675 30.449816459117407 31.892492721404793 19.83637592380312 96-97 17.95970105488728 29.845141175771552 31.50928056368863 20.685877205652538 98-99 18.238192841915694 29.933398677055322 31.049491347914742 20.778917133114245 100-101 18.987792327088552 29.086638883831267 31.964813774671192 19.960755014408985 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5425.0 1 3264.5 2 1413.5 3 1630.0 4 1372.0 5 1388.5 6 2300.5 7 4328.5 8 6559.0 9 7171.0 10 5955.0 11 5031.5 12 5169.5 13 5366.0 14 5600.5 15 5921.5 16 6157.5 17 6425.5 18 6746.0 19 7171.0 20 7737.5 21 8538.5 22 9469.5 23 10351.0 24 11161.5 25 13034.0 26 16694.0 27 20212.5 28 20771.5 29 21488.5 30 24553.0 31 27416.0 32 31049.0 33 35546.5 34 41433.0 35 44448.5 36 45461.5 37 48868.0 38 46284.0 39 40392.5 40 36572.0 41 33610.0 42 31136.5 43 29652.0 44 28420.0 45 26324.0 46 25658.5 47 28484.5 48 48944.0 49 47999.0 50 24394.0 51 15446.0 52 9914.0 53 7402.0 54 5888.0 55 4737.0 56 3853.0 57 3114.5 58 2504.5 59 2061.0 60 1719.0 61 1454.5 62 1221.0 63 849.0 64 510.0 65 389.5 66 336.0 67 272.5 68 208.5 69 161.0 70 140.5 71 116.0 72 92.5 73 94.5 74 90.0 75 86.5 76 68.5 77 39.0 78 32.5 79 27.5 80 18.5 81 15.0 82 12.5 83 8.5 84 5.0 85 3.0 86 2.0 87 1.5 88 0.5 89 0.5 90 1.5 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39637201892207946 2 0.126454685309444 3 0.12751167735990293 4 0.1399073114061934 5 0.14038776233822015 6 0.0772565098699035 7 0.08619289720560129 8 0.0833101916134407 9 0.08148447807173902 10-11 0.08061966639409084 12-13 0.08407891310468353 14-15 0.07884199794559181 16-17 0.0802833507416721 18-19 0.08950800863658596 20-21 0.08773034018808694 22-23 0.0973393588286222 24-25 0.11189702206903313 26-27 0.11319423958550537 28-29 0.10617965597791462 30-31 0.09671477261698741 32-33 0.09455274342286697 34-35 0.34438722807678374 36-37 0.535654744116638 38-39 0.5153797147851087 40-41 0.4860241628382735 42-43 0.5160043009967435 44-45 0.5258055000100894 46-47 0.5248445981460359 48-49 0.5310424151691812 50-51 0.5304178289575464 52-53 0.5287362506954527 54-55 0.5213853514354433 56-57 0.4897716801080822 58-59 0.4796822105355202 60-61 0.427024788385387 62-63 0.5069237783814377 64-65 0.5339251207613418 66-67 0.5456961685959975 68-69 0.5621275904713128 70-71 0.5553532323297354 72-73 0.5429095531902423 74-75 0.5449754921979573 76-77 0.5206166299442004 78-79 0.4920778445818107 80-81 0.49966896930783355 82-83 0.5138422718026231 84-85 0.5074042293134644 86-87 0.5094221232279769 88-89 0.5214814416218486 90-91 0.5409877494621352 92-93 0.5462246646212269 94-95 0.5413240651145539 96-97 0.5428615080970396 98-99 0.5428615080970396 100-101 0.5186467811228908 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1040689.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.25574080505436 #Duplication Level Percentage of deduplicated Percentage of total 1 77.00095240908604 25.60723715058891 2 11.67477241700412 7.765064109157738 3 3.9952933624015725 3.9859932150054243 4 1.996667673924232 2.6560265055142014 5 1.0680927936255453 1.7760108550278775 6 0.6806687352361857 1.3581685819871256 7 0.4569739305890901 1.0637904613236375 8 0.3549652436792232 0.9443705710879364 9 0.2641242284399956 0.7905282193201915 >10 1.905763379005155 13.085379428166597 >50 0.3250770383458475 7.6109667151375735 >100 0.241270222398005 15.656474237014784 >500 0.021749118605558632 4.935587102862824 >1k 0.011599529922964605 6.53327114383917 >5k 0.0014499412403705756 3.211955373257571 >10k+ 5.799764961482302E-4 3.0191763307084245 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17932 1.7230892226207828 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13375 1.285206243171591 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9531 0.9158355666294157 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7011 0.6736882968879272 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5958 0.5725053306030908 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5560 0.5342614364137606 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5246 0.5040891178824798 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3984 0.3828233026389248 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3448 0.3313189627256558 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3198 0.30729641612431763 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2929 0.2814481559812778 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2655 0.2551194449062112 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2559 0.24589478701129733 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2480 0.23830366228527444 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2379 0.22859855345833385 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2361 0.2268689301030375 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2162 0.20774698300837233 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1923 0.18478142845749307 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1789 0.1719053434791758 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1684 0.16181587390661378 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1618 0.15547392160386053 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1548 0.14874760855548583 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1492 0.1433665581167861 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1483 0.1425017464391379 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1452 0.139522950660572 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1443 0.1386581389829238 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1433 0.13769723711887027 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1421 0.13654415488200605 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1397 0.1342379904082776 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1364 0.13106701425690095 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1338 0.1285686694103618 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1326 0.12741558717349755 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1299 0.12482115214055305 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1292 0.12414852083571556 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1251 0.12020882319309613 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1216 0.11684566666890876 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1162 0.11165679660301975 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1159 0.11136852604380366 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1144 0.10992717324772337 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1090 0.10473830318183433 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1078 0.10358522094497012 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 1051 0.10099078591202558 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1051 0.10099078591202558 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.60901864053526E-5 0.0 14-15 0.0 0.0 0.0 3.363156524187341E-4 0.0 16-17 0.0 0.0 0.0 5.765411184321156E-4 0.0 18-19 0.0 0.0 0.0 6.726313048374682E-4 0.0 20-21 0.0 0.0 0.0 0.0010569920504588787 0.0 22-23 0.0 0.0 0.0 0.0030748859649712837 0.0 24-25 0.0 0.0 0.0 0.005188870065889041 0.0 26-27 0.0 0.0 0.0 0.009560973547332584 0.0 28-29 0.0 0.0 0.0 0.022581193805257863 0.0 30-31 0.0 0.0 0.0 0.055347947369483105 0.0 32-33 0.0 0.0 0.0 0.11059980455256085 0.0 34-35 0.0 0.0 0.0 0.19347759032717748 0.0 36-37 0.0 0.0 0.0 0.3151758114095565 0.0 38-39 0.0 0.0 0.0 0.49760303030011843 0.0 40-41 0.0 0.0 0.0 0.7062628700793416 0.0 42-43 0.0 0.0 0.0 0.9035360227695306 0.0 44-45 0.0 0.0 0.0 1.1218048811892891 0.0 46-47 0.0 0.0 0.0 1.3902328169126417 0.0 48-49 0.0 0.0 0.0 1.6967124664525137 0.0 50-51 0.0 0.0 0.0 2.0220738376210377 0.0 52-53 0.0 0.0 0.0 2.3194729645456036 0.0 54-55 0.0 0.0 0.0 2.6300844921009063 0.0 56-57 0.0 0.0 0.0 3.0008004312527565 0.0 58-59 0.0 0.0 0.0 3.4110574821104094 0.0 60-61 0.0 0.0 0.0 3.9082761516649067 0.0 62-63 0.0 0.0 0.0 4.375178367408515 0.0 64-65 0.0 0.0 0.0 4.838285020789112 0.0 66-67 0.0 0.0 0.0 5.347322783271467 0.0 68-69 0.0 0.0 0.0 5.919395708035734 0.0 70-71 0.0 0.0 0.0 6.572328524660105 0.0 72-73 0.0 0.0 0.0 7.223675853208787 0.0 74-75 0.0 0.0 0.0 7.890061295929908 0.0 76-77 0.0 0.0 0.0 8.560578616666458 0.0 78-79 0.0 0.0 0.0 9.329780558841307 0.0 80-81 0.0 0.0 0.0 10.15293714068276 0.0 82-83 0.0 0.0 0.0 10.968742823264204 0.0 84-85 0.0 0.0 0.0 11.823416986246611 0.0 86-87 0.0 0.0 0.0 12.692840992842243 0.0 88-89 0.0 0.0 0.0 13.571057251493961 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGGAT 30 1.4796551E-6 47.561337 94-95 ACCGAGC 45 9.786324E-4 42.07954 8 GGAACGC 50 0.0016428374 37.871586 8 CCCGTGC 35 2.0383885E-4 33.972385 88-89 GTAAGCG 35 2.0383885E-4 33.972385 94-95 GCTTATG 140 1.70985E-8 30.527946 1 TAGACCG 65 0.0059259385 29.147406 4 CCGAGCC 65 0.0059426758 29.130589 9 TAGGCAT 410 0.0 27.722914 5 CATGGGT 1320 0.0 27.629313 4 CATTCCG 395 0.0 27.563435 9 CCGTCAA 35 0.008340432 27.177908 86-87 CGGCACT 35 0.008342398 27.176592 48-49 GATTGGC 585 0.0 25.893854 9 TTAGGCA 495 0.0 25.8352 4 GGATATG 625 0.0 25.07362 1 ATGGGTA 620 0.0 24.443861 5 GAGTACT 12520 0.0 24.332018 12-13 GTACATG 12550 0.0 24.179108 1 CATGGGG 4000 0.0 24.037502 4 >>END_MODULE