##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939120_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1040689 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4096757052299 34.0 31.0 34.0 31.0 34.0 2 32.60053387707567 34.0 31.0 34.0 31.0 34.0 3 32.662779178025325 34.0 31.0 34.0 31.0 34.0 4 36.15095768284281 37.0 35.0 37.0 35.0 37.0 5 36.152850659514996 37.0 35.0 37.0 35.0 37.0 6 36.239787294763374 37.0 37.0 37.0 35.0 37.0 7 36.20110042481471 37.0 37.0 37.0 35.0 37.0 8 36.201827827525804 37.0 37.0 37.0 35.0 37.0 9 37.8763136729609 39.0 38.0 39.0 35.0 39.0 10-11 37.82081005948943 39.0 38.0 39.0 35.0 39.0 12-13 37.74734815108067 39.0 38.0 39.0 35.0 39.0 14-15 38.98220746063426 40.0 38.0 41.0 36.0 41.0 16-17 39.10732120739241 40.0 38.5 41.0 36.0 41.0 18-19 39.295551312639994 41.0 39.0 41.0 36.0 41.0 20-21 39.32080285272545 41.0 39.0 41.0 36.0 41.0 22-23 39.226278455907575 41.0 39.0 41.0 35.0 41.0 24-25 39.12955599607568 41.0 39.0 41.0 35.0 41.0 26-27 38.91660476857159 40.5 38.0 41.0 35.0 41.0 28-29 38.717245017483606 40.0 38.0 41.0 35.0 41.0 30-31 38.34794352587565 40.0 37.5 41.0 35.0 41.0 32-33 37.93898080982888 40.0 36.5 41.0 34.0 41.0 34-35 37.69427658022714 40.0 35.0 41.0 33.0 41.0 36-37 37.410615947703874 40.0 35.0 41.0 33.0 41.0 38-39 37.15311875113507 40.0 35.0 41.0 33.0 41.0 40-41 36.8412777496447 40.0 35.0 41.0 33.0 41.0 42-43 36.43908218497553 40.0 35.0 41.0 31.0 41.0 44-45 35.800681087241244 40.0 35.0 41.0 29.0 41.0 46-47 34.90852838840422 40.0 35.0 41.0 21.5 41.0 48-49 34.084896640591 39.0 35.0 41.0 4.5 41.0 50-51 33.65975714166288 39.0 35.0 41.0 2.0 41.0 52-53 33.35481445465456 39.0 34.0 41.0 2.0 41.0 54-55 33.126107799736516 39.0 34.0 40.0 2.0 41.0 56-57 32.93936276831984 38.5 34.0 40.0 2.0 41.0 58-59 32.65112584066902 38.0 33.5 40.0 2.0 41.0 60-61 32.27651872941868 38.0 33.0 40.0 2.0 41.0 62-63 31.886081240409 37.5 32.5 40.0 2.0 41.0 64-65 31.437823883984553 37.0 32.0 40.0 2.0 41.0 66-67 31.010673217454972 36.0 31.0 39.0 2.0 41.0 68-69 30.519954088108936 36.0 31.0 39.0 2.0 40.0 70-71 30.032058088439484 35.0 30.0 38.5 2.0 40.0 72-73 29.45971899385888 35.0 29.0 37.5 2.0 40.0 74-75 28.91396565160197 35.0 29.0 37.0 2.0 39.0 76-77 26.96172007199077 32.5 26.0 35.0 2.0 37.5 78-79 27.783768733982967 34.0 26.0 36.0 2.0 38.0 80-81 27.734703643451596 34.0 26.5 36.0 2.0 37.5 82-83 27.31818151244032 34.0 26.0 35.0 2.0 37.0 84-85 26.850872835208214 34.0 25.5 35.0 2.0 36.5 86-87 26.198747176149645 33.5 23.5 35.0 2.0 36.0 88-89 25.4928052472929 33.0 19.0 35.0 2.0 35.5 90-91 24.922970743420947 32.0 15.0 35.0 2.0 35.0 92-93 24.51522116597754 32.0 8.5 34.0 2.0 35.0 94-95 24.027791203712155 32.0 2.0 34.0 2.0 35.0 96-97 23.28733271899674 31.0 2.0 34.0 2.0 35.0 98-99 22.265840707454387 31.0 2.0 34.0 2.0 35.0 100-101 17.072849333470423 22.5 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19733596096797612 1101 2 0.17045965722683576 1101 3 0.258483758363937 1101 4 0.13625934563997788 1101 5 0.16028583188712986 1101 6 0.23829141196866033 1101 7 0.2413511288479384 1101 8 0.21858003473229815 1101 9 0.21600540079644048 1101 10-11 0.17822698712355134 1101 12-13 0.1439695841154034 1101 14-15 0.2330431494048426 1101 16-17 0.32043125428202046 1101 18-19 0.21297243663401844 1101 20-21 0.363339615324044 1101 22-23 0.2869599215971732 1101 24-25 0.25398904675844847 1101 26-27 0.22496772638479 1101 28-29 0.34225466575981045 1101 30-31 0.44395537736499335 1101 32-33 0.45086020702864005 1101 34-35 0.5047709220346377 1101 36-37 0.6576532952395127 1101 38-39 0.4466301375872632 1101 40-41 0.6082256581264502 1101 42-43 0.5392640960604282 1101 44-45 0.4536679529007941 1101 46-47 0.24323950022515106 1101 48-49 0.4400587580241151 1101 50-51 0.4467622753246516 1101 52-53 0.42238848047168176 1101 54-55 0.5167759325926014 1101 56-57 0.5823880502550765 1101 58-59 0.6041839606905057 1101 60-61 0.7959387274913183 1101 62-63 0.6263611704286376 1101 64-65 0.5647375463247108 1101 66-67 0.5091759679969527 1101 68-69 0.7006050885795894 1101 70-71 0.4615087205960222 1101 72-73 0.49728892441940786 1101 74-75 0.2832598833696416 1101 76-77 0.2428178124060416 1101 78-79 0.45795163028586217 1101 80-81 0.6242378397906769 1101 82-83 0.6244130736405928 1101 84-85 0.5509244144787537 1101 86-87 0.8747113976997305 1101 88-89 0.7971086120958368 1101 90-91 0.6894896789983136 1101 92-93 0.5893913827050632 1101 94-95 0.7474646888123786 1101 96-97 0.8197201056704237 1101 98-99 0.90711925627771 1101 100-101 0.5501057900844586 1102 1 0.15850112671316907 1102 2 0.17038856800583346 1102 3 0.09346019161652208 1102 4 0.12507687704665926 1102 5 0.018136898627837184 1102 6 0.08432691365736389 1102 7 0.23823393357255185 1102 8 0.1906956231230268 1102 9 0.3485210739577127 1102 10-11 0.3527201746300932 1102 12-13 0.3013215508724727 1102 14-15 0.34194580009387465 1102 16-17 0.34036373293513833 1102 18-19 0.25278307104495923 1102 20-21 0.311291575106317 1102 22-23 0.21377558054060586 1102 24-25 0.2609021381738188 1102 26-27 0.28396775589352075 1102 28-29 0.3123301276385533 1102 30-31 0.2785656340641509 1102 32-33 0.4347356962700246 1102 34-35 0.35422340090236304 1102 36-37 0.3330988100461951 1102 38-39 0.3169204766962608 1102 40-41 0.23318441296521542 1102 42-43 0.34721790684506004 1102 44-45 0.6592217647584775 1102 46-47 0.5690581663767631 1102 48-49 0.6724983791319801 1102 50-51 0.6641303109282788 1102 52-53 0.4672947365913842 1102 54-55 0.3941282480892454 1102 56-57 0.6311069688404771 1102 58-59 0.37925565349922863 1102 60-61 0.4140340192387981 1102 62-63 0.2432620291327794 1102 64-65 0.18669177559683803 1102 66-67 0.3378241192748135 1102 68-69 0.4430823735615199 1102 70-71 0.30782321394448786 1102 72-73 0.2514188057674751 1102 74-75 0.08291033901130973 1102 76-77 0.07951715328824704 1102 78-79 0.15859290871149057 1102 80-81 0.320866601148186 1102 82-83 0.22857487807280208 1102 84-85 0.20856811530312314 1102 86-87 0.01428971790167921 1102 88-89 -0.2282796289422251 1102 90-91 0.24173019663514594 1102 92-93 0.0184550705992379 1102 94-95 -0.15967333962867514 1102 96-97 -0.22541819639706517 1102 98-99 -0.003732177980857898 1102 100-101 -0.5121375174689717 1103 1 0.015189137886487458 1103 2 0.07691593645042616 1103 3 0.1721876223981198 1103 4 0.17970855608209035 1103 5 0.17009716075889258 1103 6 0.21025424238512613 1103 7 0.1968008662893581 1103 8 0.15949292658719827 1103 9 0.13785355508893105 1103 10-11 0.11439554086000214 1103 12-13 0.2016323153161821 1103 14-15 0.09020818891634264 1103 16-17 0.31504228308683224 1103 18-19 0.2730564069822421 1103 20-21 0.27031617192134405 1103 22-23 0.28087926381600425 1103 24-25 0.1918461492840109 1103 26-27 0.16340022610918936 1103 28-29 0.23840488207151367 1103 30-31 0.13716649705030193 1103 32-33 0.3237948940690316 1103 34-35 0.3039566274474055 1103 36-37 0.4577793986443055 1103 38-39 0.5425561103177401 1103 40-41 0.5543561119642675 1103 42-43 0.4071317923474993 1103 44-45 0.54561454313464 1103 46-47 0.7413647539780115 1103 48-49 0.9436623846397296 1103 50-51 0.9865507227470616 1103 52-53 1.0072548619492636 1103 54-55 1.0292410793861109 1103 56-57 1.0234743679012155 1103 58-59 1.1349011637223612 1103 60-61 1.0724601018839977 1103 62-63 0.8527147654511822 1103 64-65 1.1032246281198361 1103 66-67 1.152717403317201 1103 68-69 1.0648328598296253 1103 70-71 0.8729930033795483 1103 72-73 0.9753263628801392 1103 74-75 0.8774717639774146 1103 76-77 0.8702245994042208 1103 78-79 0.8308271643431944 1103 80-81 1.0571139922655561 1103 82-83 1.055458172412628 1103 84-85 0.8035735270495756 1103 86-87 0.9581594519699053 1103 88-89 1.4545026005394952 1103 90-91 1.3801538594471765 1103 92-93 1.1864506728154929 1103 94-95 1.0940816759358505 1103 96-97 0.9214544980214043 1103 98-99 1.0034727437493736 1103 100-101 0.3711370954563584 1104 1 0.18550108169448265 1104 2 0.21942266715698366 1104 3 0.2978387587218734 1104 4 0.16813349554333 1104 5 0.2662986357487398 1104 6 0.19384861516348906 1104 7 0.23866661313456916 1104 8 0.1866752046493616 1104 9 0.17282816139900348 1104 10-11 0.28119861818417036 1104 12-13 0.24982643039747643 1104 14-15 0.17018201812435763 1104 16-17 0.23958003269954276 1104 18-19 0.4439417769078915 1104 20-21 0.360773985306551 1104 22-23 0.3143573219632927 1104 24-25 0.2670997101661996 1104 26-27 0.4232230468392615 1104 28-29 0.36048636247600996 1104 30-31 0.45450552411851675 1104 32-33 0.4148480679042521 1104 34-35 0.3081761663000009 1104 36-37 0.535248327095772 1104 38-39 0.5831697201322825 1104 40-41 0.5719706342470445 1104 42-43 0.5942404211885162 1104 44-45 0.6499047732069769 1104 46-47 0.5328143752034222 1104 48-49 0.687015028541488 1104 50-51 0.2353029725828648 1104 52-53 0.25297892052772397 1104 54-55 0.1436474235470655 1104 56-57 0.2627465992871123 1104 58-59 0.24814686835338762 1104 60-61 0.3425099617549492 1104 62-63 0.2703726550425287 1104 64-65 0.2702589559766899 1104 66-67 0.36391682274675574 1104 68-69 0.25535259070163363 1104 70-71 0.21131441279200658 1104 72-73 -0.017964238195769866 1104 74-75 0.0017372771906565276 1104 76-77 0.03646803790536168 1104 78-79 0.1374741444243135 1104 80-81 0.14309720184747476 1104 82-83 0.23934997473791597 1104 84-85 0.06842745692989993 1104 86-87 0.14297249625469632 1104 88-89 0.23119480294700878 1104 90-91 0.24916390662479415 1104 92-93 0.22591179013785023 1104 94-95 0.5412619235055267 1104 96-97 0.03805176863842519 1104 98-99 -0.16167209804768845 1104 100-101 -0.4527947652267912 1105 1 0.019297885055173936 1105 2 0.02049919779341991 1105 3 0.04924931967470059 1105 4 -0.0464981702461813 1105 5 0.13803123132314 1105 6 0.08173746770178525 1105 7 0.09503831846720345 1105 8 0.13343304807946055 1105 9 0.16914410808691116 1105 10-11 0.23538916955940437 1105 12-13 0.18123879307521662 1105 14-15 0.13708480558245384 1105 16-17 0.20933179767875032 1105 18-19 0.20686144842498777 1105 20-21 0.17833807692900194 1105 22-23 0.2580368235492827 1105 24-25 0.19309148861093206 1105 26-27 0.2718544471114157 1105 28-29 0.3496084483044939 1105 30-31 0.4466520185782201 1105 32-33 0.5075983772793933 1105 34-35 0.4658158355412354 1105 36-37 0.5243876004316661 1105 38-39 0.37317749599770167 1105 40-41 0.48098767477162596 1105 42-43 0.44934617769962415 1105 44-45 0.7323437634824117 1105 46-47 0.9535349229277941 1105 48-49 0.555872568997323 1105 50-51 0.4720822233497799 1105 52-53 0.5299153006727124 1105 54-55 0.544618339913832 1105 56-57 0.6071676529168712 1105 58-59 0.7071451804216267 1105 60-61 0.5896704669965445 1105 62-63 0.32958124737381667 1105 64-65 0.2608473139889149 1105 66-67 0.2791604823345395 1105 68-69 0.16015928458383044 1105 70-71 0.21791085960082057 1105 72-73 0.12594261262615092 1105 74-75 0.2017897879891528 1105 76-77 0.06328647618917316 1105 78-79 -0.0036056577652594513 1105 80-81 0.02117554369158725 1105 82-83 0.011236783740852019 1105 84-85 0.04570912817199613 1105 86-87 -0.21660379020060105 1105 88-89 -0.25529344553930855 1105 90-91 -0.05643293846005193 1105 92-93 -0.04048094322108753 1105 94-95 0.08354615509355412 1105 96-97 0.26727799890835513 1105 98-99 -0.10971663384274422 1105 100-101 -0.8416407841259392 1106 1 0.06567475553627844 1106 2 -0.07030533763652613 1106 3 0.05714433571304056 1106 4 -0.006936182801453583 1106 5 0.029671430780929597 1106 6 -0.09694158719876356 1106 7 0.04532849839907982 1106 8 0.018555203559927236 1106 9 -0.012843065756939609 1106 10-11 0.03550982391458746 1106 12-13 0.10839527804929361 1106 14-15 0.12180436603491529 1106 16-17 0.08147260548731339 1106 18-19 0.07655192316432391 1106 20-21 0.04745180353459233 1106 22-23 0.137210911192426 1106 24-25 0.04788301278355789 1106 26-27 0.01461970046299399 1106 28-29 0.03536085066927086 1106 30-31 0.05539500109969708 1106 32-33 0.04779185314061607 1106 34-35 -0.15844291331034555 1106 36-37 -0.36706467619976024 1106 38-39 -0.0991651172243877 1106 40-41 -0.04561440964804575 1106 42-43 -0.13976987395714957 1106 44-45 -0.08520743843362055 1106 46-47 -0.2552840187901637 1106 48-49 -0.31599566606309537 1106 50-51 -0.10442889062061766 1106 52-53 -0.14791781934282255 1106 54-55 -0.006761340944670735 1106 56-57 -0.08741091462586326 1106 58-59 -0.3051956495087751 1106 60-61 -0.311294203512098 1106 62-63 -0.423404637533892 1106 64-65 -0.13852142332770612 1106 66-67 -0.07055035353264927 1106 68-69 -0.23414005108358182 1106 70-71 -0.15262216402666695 1106 72-73 -0.1761227325988095 1106 74-75 -0.16012869526483442 1106 76-77 -0.25330535994663705 1106 78-79 -0.08279340226244969 1106 80-81 0.05051238491767762 1106 82-83 0.05385237603071502 1106 84-85 -0.19175647574311583 1106 86-87 -0.1859898638212023 1106 88-89 -0.24470148299294436 1106 90-91 0.2243373081082396 1106 92-93 0.12300232205370776 1106 94-95 -0.07694727494384779 1106 96-97 -0.08192775909219563 1106 98-99 0.11320216318028997 1106 100-101 -0.6252168934349456 1107 1 -0.03463232642217662 1107 2 0.007689029348668441 1107 3 -0.03280482304345611 1107 4 -0.05488886378165603 1107 5 -0.053523620304034125 1107 6 8.753546548732061E-4 1107 7 0.02800242143997167 1107 8 0.0842609940363559 1107 9 0.07893502143399189 1107 10-11 0.05186474623280901 1107 12-13 -0.009296690560113063 1107 14-15 -0.06964767174740416 1107 16-17 -0.033266162921187004 1107 18-19 0.12532529074502463 1107 20-21 0.06637527888008066 1107 22-23 0.0071959279664355336 1107 24-25 -0.13004650459549794 1107 26-27 0.016152461170065635 1107 28-29 0.05748812967728867 1107 30-31 -0.0016922555288374497 1107 32-33 -0.2217477994265593 1107 34-35 -0.383037126436669 1107 36-37 -0.21861047040610515 1107 38-39 -0.22186279726151525 1107 40-41 -0.24386929826989956 1107 42-43 -0.3143414109140039 1107 44-45 -0.36942037402269534 1107 46-47 -0.43682159023600065 1107 48-49 -0.5766635322355498 1107 50-51 -0.5420079658143706 1107 52-53 -0.7345243623713742 1107 54-55 -0.7398135533546863 1107 56-57 -0.8409707553416652 1107 58-59 -0.6177177693703442 1107 60-61 -0.7545820573504152 1107 62-63 -0.6298266558825176 1107 64-65 -0.6555163646345719 1107 66-67 -0.5645109690528933 1107 68-69 -0.45794960516836625 1107 70-71 -0.3274427149940067 1107 72-73 -0.5969685961052207 1107 74-75 -0.5413527209842748 1107 76-77 -0.585247393723531 1107 78-79 -0.41568433836439667 1107 80-81 -0.3000913945851842 1107 82-83 -0.43210694249741266 1107 84-85 -0.5365741233130557 1107 86-87 -0.29301820479449603 1107 88-89 -0.7078712512085872 1107 90-91 -0.3144558958225794 1107 92-93 -0.11151960283518747 1107 94-95 -0.04358098699040269 1107 96-97 -0.4848578592700008 1107 98-99 -0.4465214630571026 1107 100-101 -1.0864883271834422 1108 1 -0.14599520914573105 1108 2 0.035350132476779095 1108 3 0.004451234827165251 1108 4 -0.004368740474973265 1108 5 -0.12140063294174297 1108 6 0.09136882701526616 1108 7 -0.021882132064568793 1108 8 -0.009686662031334947 1108 9 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98.0 7 187.0 8 425.0 9 856.0 10 1724.0 11 3114.0 12 5247.0 13 7607.0 14 9215.0 15 13112.0 16 18867.0 17 30716.0 18 28941.0 19 13631.0 20 8965.0 21 8407.0 22 8505.0 23 8787.0 24 9652.0 25 10525.0 26 11516.0 27 13482.0 28 15595.0 29 18377.0 30 21884.0 31 26075.0 32 31836.0 33 40670.0 34 56397.0 35 85805.0 36 126542.0 37 224206.0 38 170044.0 39 9619.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.61520050647766 22.502837355816 16.016391037815968 16.865571099890367 2 14.489150937503902 24.440635002387843 41.28582122036459 19.78439283974367 3 18.87979982492368 31.81267410340649 33.00044489756306 16.30708117410677 4 10.293372948114182 20.310967061245 41.287935204465505 28.107724786175314 5 9.221198648203258 42.583711368141685 34.72295757906541 13.472132404589651 6 22.55793998014777 42.148230643352626 19.20237458068645 16.09145479581316 7 22.274185659692762 39.94593966112835 22.142542104317428 15.637332574861462 8 24.827205822296573 33.83700606040806 20.78651739376509 20.549270723530277 9 26.980010358522094 18.176419660436498 23.660670959335594 31.18289902170581 10-11 22.340151572660037 29.784066133109892 27.48496428808222 20.39081800614785 12-13 26.09199290085703 29.425841918190738 28.2618534451695 16.220311735782737 14-15 22.67183699904197 28.76726874037289 31.08454944406595 17.47634481651918 16-17 16.138774792452324 32.35596102298099 31.922103617448272 19.583160567118412 18-19 15.802319424919453 29.828363709042762 38.02375157227568 16.345565293762114 20-21 17.986257181540307 27.612572055628533 37.79342339546204 16.607747367369118 22-23 17.26899198511755 28.016198883624217 37.32993238133583 17.384876749922405 24-25 17.018773139718014 29.013326747952554 38.093993498537984 15.87390661379144 26-27 17.049190724866634 27.819586442557487 39.348495921072676 15.782726911503204 28-29 15.64035941573323 28.79635510704927 39.428205736776306 16.13507974044119 30-31 16.25245390313533 29.107350995350195 38.670246346410885 15.96994875510359 32-33 15.958369887641746 29.51640691887778 38.241443889577006 16.283779303903472 34-35 16.654029410690637 28.79367541376202 38.1067564632597 16.44553871228764 36-37 17.303852332924684 28.41454160645469 38.18471648269475 16.096889577925868 38-39 16.714881454093916 29.425372639437647 37.81325595901134 16.046489947457093 40-41 16.12554481763174 29.576672315648512 38.448040338247004 15.849742528472744 42-43 15.757376570260007 30.350560065808697 37.02997908882636 16.86208427510494 44-45 16.53217533857461 30.13641961102177 36.20191044150792 17.129494608895694 46-47 17.342951810239125 30.74792722911758 34.51821770532636 17.390903255316942 48-49 17.372645602449985 30.807594034164893 34.1713279758307 17.648432387554426 50-51 17.17320803124865 31.36669597428617 34.03484243551122 17.42525355895396 52-53 16.891021236892097 32.4267864847231 33.45447102832835 17.22772125005645 54-55 16.405237299519836 31.53170639835724 33.724100091381764 18.338956210741152 56-57 17.22663276601633 32.13806635610288 32.779613102101685 17.8556877757791 58-59 17.145660278003195 31.218112535992386 33.25775713085253 18.37847005515189 60-61 16.928742678960088 31.538631548096653 33.64987772241788 17.882748050525386 62-63 17.30793068756072 31.122421034728287 33.48174605233699 18.087902225374012 64-65 16.827197726828295 31.68415636031155 33.448228312691796 18.040417600168354 66-67 16.48029634754533 31.468477038830756 32.79425754561965 19.256969068004267 68-69 16.165688991047407 31.723679993773114 32.96814438304577 19.14248663213371 70-71 16.75330815125009 31.97507992316614 32.507036217421756 18.764575708162013 72-73 16.90456995317525 30.823329544176982 32.69843344169103 19.57366706095673 74-75 16.280848553218107 32.42793956695997 31.86773378021676 19.423478099605166 76-77 16.636377932232424 31.10538411709742 32.70481203752373 19.553425913146434 78-79 16.25411149633239 31.08370513994104 32.820468464312576 19.841714899413997 80-81 16.343386628256145 31.551081160810117 32.1681735238357 19.93735868709804 82-83 16.022606177253724 31.825021692359577 32.079228280494945 20.073143849891753 84-85 15.892067659022052 31.23728606721124 31.706542492521784 21.164103781244926 86-87 16.040229108340696 31.230752730268645 31.995954195646288 20.73306396574437 88-89 16.739615839619894 30.49839917092686 31.77380352742059 20.988181462032657 90-91 15.94706072789328 30.370797808976864 32.40752489941568 21.274616563714183 92-93 16.1658286000909 31.308921301176433 31.857740400830604 20.667509697902062 94-95 15.96024364627665 30.762120095436774 31.595798552689615 21.681837705596966 96-97 15.935740648743286 30.283014426019683 31.28350544687222 22.497739478364814 98-99 16.07869797609753 30.426665065161252 30.665593658038553 22.82904330070266 100-101 16.376986784716664 29.70464759404587 31.73301533887646 22.185350282361014 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4043.0 1 2595.0 2 1349.5 3 1487.0 4 1203.0 5 1206.0 6 2148.5 7 4067.0 8 6107.0 9 6776.5 10 5690.0 11 4653.0 12 4721.0 13 5035.0 14 5325.5 15 5511.0 16 5842.5 17 6337.0 18 6787.5 19 7302.0 20 7888.0 21 8590.5 22 9501.5 23 10415.0 24 11322.0 25 13144.5 26 16676.0 27 20381.0 28 21214.0 29 22049.0 30 24958.5 31 27897.0 32 31491.5 33 35698.0 34 41441.5 35 44386.0 36 45492.5 37 48529.5 38 46217.5 39 40793.5 40 36979.0 41 33917.0 42 31095.5 43 29663.5 44 28812.0 45 26983.5 46 25836.0 47 28886.0 48 48703.5 49 47889.0 50 26182.0 51 16925.5 52 10187.0 53 7141.0 54 5534.0 55 4422.5 56 3496.0 57 2807.0 58 2314.5 59 1938.5 60 1611.0 61 1331.5 62 1079.0 63 715.0 64 422.0 65 327.0 66 259.5 67 176.0 68 126.0 69 99.0 70 83.5 71 81.0 72 69.5 73 78.5 74 75.0 75 60.0 76 48.0 77 24.5 78 16.0 79 15.5 80 11.5 81 6.5 82 4.5 83 2.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.43365501124735634 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.687214912428209E-4 16-17 0.002354209566931139 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.003411201617390018 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011050371436615549 36-37 0.02570412486343182 38-39 0.019794578399502636 40-41 0.008503981496873706 42-43 0.009320748081319203 44-45 0.0037955623630114284 46-47 0.0050927798794836885 48-49 0.002882705592160578 50-51 3.8436074562141047E-4 52-53 0.0 54-55 0.0 56-57 9.609018640535262E-5 58-59 0.0016815782620936705 60-61 0.0023061644737284626 62-63 9.609018640535261E-4 64-65 0.0027385703125525494 66-67 0.0018257135417016994 68-69 0.0038916525494167805 70-71 1.9218037281070523E-4 72-73 0.0 74-75 0.0 76-77 0.09354379646561076 78-79 0.015518565104464446 80-81 1.9218037281070523E-4 82-83 0.0 84-85 0.0 86-87 0.10507461883425308 88-89 0.4180884010496892 90-91 0.41986606949818817 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.441352796080289E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1040689.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.191017874327365 #Duplication Level Percentage of deduplicated Percentage of total 1 76.29348191173285 24.559648399152636 2 11.864025933641631 7.638301417826823 3 4.234946644690274 4.08981729408142 4 2.0030737370951 2.5792392989769635 5 1.1551699034187226 1.859304750441856 6 0.7068327361975315 1.3652199147056674 7 0.49254150820919407 1.1098788746227226 8 0.369242631278113 0.9509036914749923 9 0.27362761312600375 0.7927516246543851 >10 1.9740898870226433 13.068064231751347 >50 0.34066795872492894 7.701310025858669 >100 0.2543812090942986 16.13143583342895 >500 0.02298985105663075 4.965943711779413 >1k 0.012838488252404185 6.893865537950027 >5k 0.0014928474712097891 3.221116310996657 >10k+ 5.971389884839157E-4 3.073199082297491 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17743 1.7049281773901712 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14232 1.3675555329209783 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9493 0.9121841395460123 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6856 0.6587943179950975 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6422 0.6170911770951745 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5530 0.5313787308215999 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5213 0.5009181417311032 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3956 0.38013277741957496 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3359 0.3227669361355794 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3336 0.3205568618482563 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2968 0.2851956732510865 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2633 0.2530054608052934 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2599 0.24973839446751142 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2443 0.23474832538827642 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2357 0.2264845693574161 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2278 0.21889344463139324 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2024 0.19448653728443369 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1975 0.18977811815057138 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1697 0.16306504632988336 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1628 0.15643482346791404 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1605 0.15422474918059095 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1590 0.15278339638451066 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1579 0.15172640433405177 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1572 0.1510537730292143 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1559 0.1498046006059447 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1509 0.14500009128567706 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1486 0.14279001699835397 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1453 0.13961904084697735 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1446 0.13894640954213985 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1435 0.137889417491681 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1434 0.13779332730527563 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1378 0.1324122768665759 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1367 0.13135528481611702 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1336 0.12837648903755108 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1300 0.12491724232695839 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1258 0.12088145449793358 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1207 0.1159808549912606 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1169 0.1123294279078572 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1143 0.10983108306131803 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1141 0.10963890268850733 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1092 0.10493048355464504 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1085 0.10425785224980758 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1074 0.1032008601993487 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 1067 0.10252822889451124 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1067 0.10252822889451124 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1049 0.10079860553921488 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1047 0.1006064251664042 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.60901864053526E-5 0.0 14-15 0.0 0.0 0.0 4.3240583882408676E-4 0.0 16-17 0.0 0.0 0.0 6.726313048374682E-4 0.0 18-19 0.0 0.0 0.0 7.687214912428208E-4 0.0 20-21 0.0 0.0 0.0 0.0010569920504588787 0.0 22-23 0.0 0.0 0.0 0.0029307506853632546 0.0 24-25 0.0 0.0 0.0 0.004996689693078336 0.0 26-27 0.0 0.0 0.0 0.009272702988116527 0.0 28-29 0.0 0.0 0.0 0.022292923246041804 0.0 30-31 0.0 0.0 0.0 0.055395992462685784 0.0 32-33 0.0 0.0 0.0 0.1108880751117769 0.0 34-35 0.0 0.0 0.0 0.19530330386887917 0.0 36-37 0.0 0.0 0.0 0.3190194188657707 0.0 38-39 0.0 0.0 0.0 0.5029840807388182 0.0 40-41 0.0 0.0 0.0 0.7139020398985672 0.0 42-43 0.0 0.0 0.0 0.9133852668760792 0.0 44-45 0.0 0.0 0.0 1.1340083348627688 0.0 46-47 0.0 0.0 0.0 1.404694389966647 0.0 48-49 0.0 0.0 0.0 1.7125193021161942 0.0 50-51 0.0 0.0 0.0 2.0404751083176627 0.0 52-53 0.0 0.0 0.0 2.3401803997159574 0.0 54-55 0.0 0.0 0.0 2.652713730999367 0.0 56-57 0.0 0.0 0.0 3.0281380892850795 0.0 58-59 0.0 0.0 0.0 3.4413258908280957 0.0 60-61 0.0 0.0 0.0 3.9419557619999828 0.0 62-63 0.0 0.0 0.0 4.413422261597845 0.0 64-65 0.0 0.0 0.0 4.879315530384197 0.0 66-67 0.0 0.0 0.0 5.393205847280023 0.0 68-69 0.0 0.0 0.0 5.970515687203381 0.0 70-71 0.0 0.0 0.0 6.6283491033344255 0.0 72-73 0.0 0.0 0.0 7.283972445178146 0.0 74-75 0.0 0.0 0.0 7.954537811007899 0.0 76-77 0.0 0.0 0.0 8.627313251124976 0.0 78-79 0.0 0.0 0.0 9.39680346385904 0.0 80-81 0.0 0.0 0.0 10.215924257871468 0.0 82-83 0.0 0.0 0.0 11.030528813122846 0.0 84-85 0.0 0.0 0.0 11.886548238714928 0.0 86-87 0.0 0.0 0.0 12.75616442568337 0.0 88-89 0.0 0.0 0.0 13.631642114022537 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 45 1.4881309E-5 52.942684 1 GTAAGCG 105 0.0 47.449234 94-95 CGACAAG 25 0.0015759409 38.19521 90-91 CATTCCG 280 0.0 35.58693 9 TCGTATC 85 3.6379788E-12 33.49358 94-95 GACATAT 340 0.0 32.23275 1 ACCGTGC 150 9.658834E-10 31.632826 7 GCTTTGC 170 1.03682396E-10 30.831326 1 TTGCGTT 155 1.371518E-9 30.612411 4 CTTTGCG 175 1.546141E-10 29.825235 2 CCGTGCA 160 1.9263098E-9 29.655775 8 CTCCGAC 305 0.0 28.780684 94-95 CGTGCAA 150 3.3707693E-8 28.469543 9 TAGGCAT 370 0.0 28.213058 5 TGACCGT 175 5.0167728E-9 27.113852 5 GTGTAGC 300 0.0 27.000767 1 GTCGGGA 90 9.002998E-4 26.36069 2 CATGGGG 3920 0.0 25.661324 4 CCTTTAG 410 0.0 25.567442 1 CTTTAGG 395 0.0 25.226177 2 >>END_MODULE