FastQCFastQC Report
Fri 27 May 2016
SRR939119_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939119_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208015
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT80240.3634033283288383No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59330.2687028847177216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47500.21512535014481332No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42430.19216354961356694No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30890.13989941191522703No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30460.13795196137707397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29420.13324184844758755No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG89300.025.6766911
TACATGG88850.025.3252012
TACCTGG19250.023.870292
ACATGGG90000.023.7909973
GTACCTG21450.022.1317671
GAGTACT60650.021.15936312-13
CATGGGG45800.020.1690674
GTACTTT65200.019.3574514-15
AGAGTAC111650.018.70419510-11
AGTACTT63900.018.60102812-13
CATGGGA47100.018.405474
GAGTACA76050.017.9778041
CAGTACG2403.1847303E-617.7578539
GTATCAA171000.017.5732061
AGTACAT68600.017.3327052
GTGTAGC11050.016.7550491
ATGGGGA28450.015.8182355
TCAACGC187500.015.765224
CAACGCA187400.015.7483555
ACTTTTT81700.015.73984516-17