FastQCFastQC Report
Fri 27 May 2016
SRR939119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208015
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT87190.39487956377107947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62170.2815651161790114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52950.2398081534772182No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39070.17694626168753383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33050.14968195415339117No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29660.13432879758516134No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27320.12373104349381685No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23160.1048905917758711No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG86700.026.4161431
TACATGG86050.025.8042722
ACATGGG85100.025.0887833
TACCTGG19850.022.2289542
CATGGGA43750.021.0387694
GTGTAGC11000.020.7774351
GAGTACT70950.020.2301512-13
CATGGGG44700.019.1056424
GTACGAT1250.00607286818.978273
GTACCTG24000.018.649191
GTACTTT75800.018.62275514-15
TAGGACG1559.702626E-418.3660684
CGTGTCG1300.007615363718.2483377
GAGTACA74000.017.179991
GTGTAGG10150.016.8880631
AGTACAT66050.016.8089142
AGAGTAC119650.016.71517810-11
AGTACTT75400.016.64487312-13
GTAGCCC11350.016.3029173
ACTTTTT88000.016.22894516-17
Produced by FastQC (version 0.11.5)