##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939119_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2208015 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49373894651984 34.0 31.0 34.0 31.0 34.0 2 32.698017450062615 34.0 31.0 34.0 31.0 34.0 3 32.79547376263295 34.0 31.0 34.0 31.0 34.0 4 36.24706942661169 37.0 35.0 37.0 35.0 37.0 5 36.20993924407217 37.0 37.0 37.0 35.0 37.0 6 36.250951193719246 37.0 37.0 37.0 35.0 37.0 7 36.20409191060749 37.0 37.0 37.0 35.0 37.0 8 36.19351544260343 37.0 37.0 37.0 35.0 37.0 9 37.93808330106453 39.0 38.0 39.0 35.0 39.0 10-11 37.87492997103733 39.0 38.0 39.0 35.0 39.0 12-13 37.81077076016241 39.0 38.0 39.0 35.0 39.0 14-15 39.17703865236423 40.5 39.0 41.0 36.0 41.0 16-17 39.12181303116147 40.0 38.0 41.0 36.0 41.0 18-19 39.143828733047556 40.5 38.5 41.0 36.0 41.0 20-21 39.21510066734148 41.0 39.0 41.0 36.0 41.0 22-23 39.174487039263774 41.0 39.0 41.0 36.0 41.0 24-25 39.11363147442386 41.0 39.0 41.0 35.0 41.0 26-27 38.978511921341116 40.0 38.5 41.0 35.0 41.0 28-29 38.832811144851824 40.0 38.0 41.0 35.0 41.0 30-31 38.657057583395044 40.0 38.0 41.0 34.5 41.0 32-33 38.523639332160336 40.0 38.0 41.0 34.0 41.0 34-35 38.47115214344105 40.0 38.0 41.0 34.0 41.0 36-37 38.367112542260806 40.0 38.0 41.0 34.0 41.0 38-39 38.23400610955994 40.0 38.0 41.0 34.0 41.0 40-41 38.08294395644957 40.0 38.0 41.0 33.0 41.0 42-43 37.92174011499016 40.0 38.0 41.0 33.0 41.0 44-45 37.7099560012047 40.0 37.5 41.0 33.0 41.0 46-47 37.43764331311155 40.0 37.0 41.0 32.5 41.0 48-49 37.13368160995283 40.0 37.0 41.0 31.5 41.0 50-51 36.882179015994005 40.0 36.5 41.0 31.0 41.0 52-53 36.660344245849785 39.5 36.0 41.0 31.0 41.0 54-55 36.420134600534865 39.0 35.5 41.0 30.0 41.0 56-57 36.14253956608084 39.0 35.0 40.5 30.0 41.0 58-59 35.84467111862918 38.5 35.0 40.0 29.0 41.0 60-61 35.47282083681497 38.0 35.0 40.0 28.0 41.0 62-63 35.090427601261766 38.0 34.0 40.0 28.0 41.0 64-65 34.681599536235034 37.0 34.0 40.0 27.5 41.0 66-67 34.316758038328544 36.5 34.0 39.0 26.5 41.0 68-69 33.84132105080808 36.0 33.5 39.0 26.0 40.0 70-71 33.38558026100367 35.5 33.0 38.5 26.0 40.0 72-73 32.908131058892266 35.0 32.5 38.0 25.0 40.0 74-75 32.41221911988822 35.0 32.0 37.0 23.5 39.0 76-77 30.42424372117037 33.5 29.5 35.5 20.0 37.5 78-79 31.40281950077332 34.5 31.0 36.0 21.5 38.0 80-81 31.423699340810636 35.0 31.5 36.0 22.0 37.5 82-83 31.129587208420233 35.0 31.0 35.0 20.5 37.0 84-85 30.749140291166498 34.0 31.0 35.0 19.0 36.5 86-87 30.198400599633608 34.0 30.5 35.0 15.0 36.0 88-89 29.656983534985045 34.0 30.0 35.0 7.0 36.0 90-91 29.198115275484994 34.0 29.0 35.0 4.0 35.0 92-93 28.910128554380293 34.0 29.0 35.0 2.0 35.0 94-95 28.5699386100185 34.0 29.0 35.0 2.0 35.0 96-97 27.996816371265595 34.0 29.0 35.0 2.0 35.0 98-99 27.21650419041537 33.5 27.0 35.0 2.0 35.0 100-101 23.53657040373367 29.5 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0979553193783147 1101 2 0.14589118220847297 1101 3 0.12152261079543791 1101 4 0.12043936653581255 1101 5 0.0883236834981389 1101 6 0.14437102537342383 1101 7 0.05908665760629361 1101 8 0.10967878957957566 1101 9 0.1335004308836858 1101 10-11 0.1984391129919203 1101 12-13 0.18651090356694766 1101 14-15 0.24562659144390153 1101 16-17 0.16056904585069987 1101 18-19 0.19488556736400398 1101 20-21 0.20968779208740074 1101 22-23 0.24420658134420137 1101 24-25 0.22119281672851088 1101 26-27 0.17882825878033515 1101 28-29 0.16611133820087787 1101 30-31 0.1569561258538954 1101 32-33 0.19747068539084012 1101 34-35 0.3153865557945963 1101 36-37 0.2833737012493174 1101 38-39 0.31781990363322876 1101 40-41 0.35639253020689665 1101 42-43 0.4312600108150946 1101 44-45 0.39014692960087416 1101 46-47 0.28716055968037324 1101 48-49 0.23058417530035058 1101 50-51 0.2985127372184948 1101 52-53 0.36056568818047907 1101 54-55 0.4155563392907027 1101 56-57 0.18564417080465034 1101 58-59 0.27092929639807295 1101 60-61 0.3135015034102864 1101 62-63 0.19002262261114566 1101 64-65 0.1368277350948901 1101 66-67 0.11317152845597889 1101 68-69 0.2709779056777748 1101 70-71 0.30598216915478105 1101 72-73 0.2353421686985513 1101 74-75 0.28493342898075724 1101 76-77 0.4651063621291982 1101 78-79 0.5164914001185323 1101 80-81 0.30153862519387076 1101 82-83 0.5440464155341367 1101 84-85 0.3097170080132905 1101 86-87 0.3865955420131719 1101 88-89 0.5912181614450738 1101 90-91 0.6768240123582707 1101 92-93 0.722605121511787 1101 94-95 0.46661546395123565 1101 96-97 0.2895993357294273 1101 98-99 0.3439631797279823 1101 100-101 -0.26845758634912187 1102 1 0.11067855780108715 1102 2 0.05823505048004307 1102 3 0.13551880914290138 1102 4 0.07223121068714278 1102 5 0.09096051012539874 1102 6 0.1002163046878124 1102 7 0.09222125052414754 1102 8 0.1309724433853603 1102 9 0.2625310730936903 1102 10-11 0.12511842356339997 1102 12-13 0.14576253670853845 1102 14-15 0.22065134565105637 1102 16-17 0.17208664307059962 1102 18-19 0.266160191704337 1102 20-21 0.23640881906366218 1102 22-23 0.18211552932750408 1102 24-25 0.11139319858997965 1102 26-27 0.2718690931508405 1102 28-29 0.23535990754552216 1102 30-31 0.2208904916903407 1102 32-33 0.18124872867412734 1102 34-35 0.19738213714211383 1102 36-37 0.20767128074371044 1102 38-39 0.2591238089672103 1102 40-41 0.23756266210919108 1102 42-43 0.1885228792923357 1102 44-45 0.23225404799913463 1102 46-47 0.10591059943552494 1102 48-49 0.09568103474946099 1102 50-51 0.10222499367844762 1102 52-53 0.1736883863572629 1102 54-55 0.23233399350358042 1102 56-57 0.06992408311008802 1102 58-59 0.17903659077913403 1102 60-61 0.10038235669364326 1102 62-63 0.1332696271930658 1102 64-65 -0.012206506281422946 1102 66-67 0.004473127991211356 1102 68-69 -0.04268559506538594 1102 70-71 -0.10957709570357821 1102 72-73 -0.1721893385835358 1102 74-75 -0.08036260377325277 1102 76-77 0.0035377988061355836 1102 78-79 0.09525072667688406 1102 80-81 -0.18096950605961482 1102 82-83 -0.20057577271657223 1102 84-85 -0.14393047405448556 1102 86-87 -0.10153781054398436 1102 88-89 -0.05974115684420411 1102 90-91 0.02007342875266005 1102 92-93 0.14968065841834388 1102 94-95 0.06301017535004405 1102 96-97 0.02575221919700965 1102 98-99 -0.0032412115729592017 1102 100-101 -0.21873489177681193 1103 1 0.09872179868337838 1103 2 0.11887770003402665 1103 3 0.1474944672476255 1103 4 0.10361612845554902 1103 5 0.12276120560812132 1103 6 0.1429658133141487 1103 7 0.12580475044855177 1103 8 0.11527998450115717 1103 9 0.21203507967894097 1103 10-11 0.14847547162562336 1103 12-13 0.16333964457556505 1103 14-15 0.23655904299847208 1103 16-17 0.24295574448676405 1103 18-19 0.17473109230406436 1103 20-21 0.17222365938821582 1103 22-23 0.1729117226515413 1103 24-25 0.12668247908454333 1103 26-27 0.21668300112536798 1103 28-29 0.2610835287594213 1103 30-31 0.1856008332160286 1103 32-33 0.21354709748155898 1103 34-35 0.2182770671738652 1103 36-37 0.3047270285556323 1103 38-39 0.20545698550894542 1103 40-41 0.20594885660916162 1103 42-43 0.17634668193651493 1103 44-45 0.20736618198875334 1103 46-47 0.3494345465519615 1103 48-49 0.3329116191901278 1103 50-51 0.2872292774488372 1103 52-53 0.3137834272630684 1103 54-55 0.26101740607266777 1103 56-57 0.2738851007995393 1103 58-59 0.2705927333698881 1103 60-61 0.24309595724465538 1103 62-63 0.28477125670571724 1103 64-65 0.22590393099914508 1103 66-67 0.19635664303480382 1103 68-69 0.17982582832232197 1103 70-71 0.12407108075417028 1103 72-73 0.2308956060634344 1103 74-75 0.06518479488618567 1103 76-77 0.0975697086957652 1103 78-79 0.27809422626530633 1103 80-81 0.22846759811159956 1103 82-83 0.22344924954223444 1103 84-85 0.1993759247225384 1103 86-87 0.42259304604210257 1103 88-89 0.4091007132315596 1103 90-91 0.45809735067044244 1103 92-93 0.5724562476467625 1103 94-95 0.3642904767258912 1103 96-97 0.2640033489364555 1103 98-99 0.19407471229352424 1103 100-101 -0.03294944987649728 1104 1 0.09797287042553648 1104 2 0.1646229134487811 1104 3 0.13753292679953688 1104 4 0.06210921328508334 1104 5 0.17989777674836205 1104 6 0.16048815203632216 1104 7 0.15672187964960216 1104 8 0.14535630764085283 1104 9 0.1704288371533238 1104 10-11 0.2434011350330323 1104 12-13 0.18002366042026097 1104 14-15 0.25206180827203895 1104 16-17 0.19647848846454252 1104 18-19 0.22673194254623041 1104 20-21 0.20852929760621208 1104 22-23 0.23477602757857596 1104 24-25 0.24779406144877925 1104 26-27 0.1282208170360235 1104 28-29 0.20196612183909224 1104 30-31 0.23753712067734511 1104 32-33 0.16450818897000374 1104 34-35 0.19487479734514324 1104 36-37 0.21316111293620565 1104 38-39 0.2819911958366035 1104 40-41 0.39274363238759236 1104 42-43 0.24395285294643543 1104 44-45 0.2542770019162006 1104 46-47 0.21523310868916923 1104 48-49 0.015296260399864536 1104 50-51 0.08393225488563161 1104 52-53 0.0214359543496343 1104 54-55 0.08856177560221568 1104 56-57 0.1648288795299777 1104 58-59 0.09551560567862083 1104 60-61 0.10951332880444653 1104 62-63 0.023556205035625055 1104 64-65 0.10013488895188516 1104 66-67 0.03765313320337782 1104 68-69 0.07505732065623505 1104 70-71 0.12197924665468918 1104 72-73 -0.08563037995921974 1104 74-75 -0.12460345905811465 1104 76-77 -0.16304937884342152 1104 78-79 0.03946547020136748 1104 80-81 -0.05909502778582265 1104 82-83 -0.04853661220203165 1104 84-85 -0.08174656378528766 1104 86-87 -0.0524283590029313 1104 88-89 0.18223902061396657 1104 90-91 0.24914686337044145 1104 92-93 0.3058335664438694 1104 94-95 0.35628469190072565 1104 96-97 0.38863111967302544 1104 98-99 0.48948643411335624 1104 100-101 0.2596786228152297 1105 1 0.043482807052534156 1105 2 0.06636108205863422 1105 3 0.035869320244337644 1105 4 0.04083863223703332 1105 5 0.03511814034747118 1105 6 0.07194879517673769 1105 7 0.06273740582197718 1105 8 0.07731155523003963 1105 9 0.07902865947554716 1105 10-11 0.0697841971144797 1105 12-13 0.08322994734852074 1105 14-15 0.11144640570572761 1105 16-17 0.08946083309965047 1105 18-19 0.07708324271019507 1105 20-21 0.1360884907233526 1105 22-23 0.12028687014402806 1105 24-25 0.10601457540520443 1105 26-27 0.11751485382661286 1105 28-29 0.15307390432469248 1105 30-31 0.061395531509518264 1105 32-33 0.11994538490552031 1105 34-35 0.24979435143369955 1105 36-37 0.19783142485887595 1105 38-39 0.22791473368277337 1105 40-41 0.27024640593475624 1105 42-43 0.22584985485934084 1105 44-45 0.2500952184886174 1105 46-47 0.26992570385183257 1105 48-49 0.22358336794708578 1105 50-51 0.319863884517126 1105 52-53 0.22886016738082304 1105 54-55 0.11399137303826024 1105 56-57 0.16990498073136706 1105 58-59 0.24354547413729932 1105 60-61 0.2581456937638862 1105 62-63 0.13613210109650709 1105 64-65 0.15583338409799552 1105 66-67 0.0514041879576439 1105 68-69 0.12410938176108033 1105 70-71 0.1035806327879456 1105 72-73 0.22358775721313862 1105 74-75 0.07524632591194091 1105 76-77 0.20887345417315117 1105 78-79 0.33307822467639525 1105 80-81 0.15568898652705698 1105 82-83 0.21948077232994478 1105 84-85 0.24401514784074863 1105 86-87 0.411046194798665 1105 88-89 0.5150619532837695 1105 90-91 0.6136016330889476 1105 92-93 0.3053873561836191 1105 94-95 0.12783630359368914 1105 96-97 0.1486032015907348 1105 98-99 0.069533210893443 1105 100-101 -0.2011157049665826 1106 1 0.07384141590825521 1106 2 0.04510147625780547 1106 3 0.07092104067420024 1106 4 0.028717343030450593 1106 5 0.005232681500650926 1106 6 0.07526246403185155 1106 7 0.09920371990015298 1106 8 0.051533204964904655 1106 9 0.07574505391522734 1106 10-11 0.10509122429400719 1106 12-13 0.06354207372535825 1106 14-15 0.08776901723476982 1106 16-17 0.018020153761696633 1106 18-19 -0.039935819731262256 1106 20-21 0.08279598180264713 1106 22-23 0.16614414812080724 1106 24-25 0.2162803811824503 1106 26-27 0.2934726588360803 1106 28-29 0.2518315964811748 1106 30-31 0.21279994988883288 1106 32-33 0.22372376292138085 1106 34-35 0.1298753373247692 1106 36-37 0.19431112866604394 1106 38-39 0.26700464837239934 1106 40-41 0.32914999757345953 1106 42-43 0.4327139988301454 1106 44-45 0.50893960856218 1106 46-47 0.4861432326027497 1106 48-49 0.37718379438335603 1106 50-51 0.44560708385295555 1106 52-53 0.4827703613815757 1106 54-55 0.45475130375393746 1106 56-57 0.5105477802253731 1106 58-59 0.4368836792660957 1106 60-61 0.4707505395045004 1106 62-63 0.5701963579202953 1106 64-65 0.5009913435865556 1106 66-67 0.6388253585298358 1106 68-69 0.5268077937253395 1106 70-71 0.43962131998861764 1106 72-73 0.5188326690004956 1106 74-75 0.5152860094610787 1106 76-77 0.44655756294677573 1106 78-79 0.5557212045501778 1106 80-81 0.5076542009824152 1106 82-83 0.7244291906537548 1106 84-85 0.6485984127608191 1106 86-87 0.6741158653280799 1106 88-89 0.8100944563305674 1106 90-91 1.1254234456281154 1106 92-93 1.0358303735208914 1106 94-95 0.9193144001708689 1106 96-97 0.9075210154804374 1106 98-99 1.1747013225253973 1106 100-101 0.5798909991481622 1107 1 -0.005131584775270426 1107 2 -0.08597336994861848 1107 3 -0.06415243847823149 1107 4 0.013209824233456402 1107 5 0.016630494214254554 1107 6 0.031593632863021526 1107 7 0.02972320041963883 1107 8 -0.03477062278629006 1107 9 -0.04992992899659754 1107 10-11 0.009034320671311491 1107 12-13 -0.01136394131223284 1107 14-15 -0.004494311541719753 1107 16-17 0.027892846858073028 1107 18-19 -0.017486229847470725 1107 20-21 0.013370998890820829 1107 22-23 -0.02501699336927743 1107 24-25 -0.04162289974714639 1107 26-27 0.07765755288939857 1107 28-29 0.05896163749279992 1107 30-31 0.12049817271863361 1107 32-33 0.16875964808884447 1107 34-35 0.09804861141909527 1107 36-37 0.1420426734370679 1107 38-39 0.09659709539905492 1107 40-41 0.17430122792206504 1107 42-43 0.03282079992379039 1107 44-45 -0.061015107774117894 1107 46-47 -0.07704029439656068 1107 48-49 0.010796234574748098 1107 50-51 -0.0671663269467686 1107 52-53 -0.10676313144139016 1107 54-55 -0.08247955749008895 1107 56-57 -0.19322016235835804 1107 58-59 -0.14400746905925388 1107 60-61 -0.11158883165066413 1107 62-63 -0.2415987548616627 1107 64-65 -0.1993418758256169 1107 66-67 0.028521189015137338 1107 68-69 -0.021573101695025798 1107 70-71 -0.061079295185678006 1107 72-73 0.05250491780432753 1107 74-75 0.21115067111521313 1107 76-77 0.07776057046557128 1107 78-79 0.2537304321646694 1107 80-81 0.1542194778108481 1107 82-83 0.11453513733864895 1107 84-85 0.13781577434659908 1107 86-87 0.12869122417975376 1107 88-89 0.17473475024717544 1107 90-91 0.23376247501978042 1107 92-93 0.3463139688729022 1107 94-95 0.17091641657551548 1107 96-97 0.23546445362124402 1107 98-99 0.13855128834904562 1107 100-101 -0.0385343255271664 1108 1 -0.04475498720399429 1108 2 -0.037044936987769006 1108 3 -0.019848827240188882 1108 4 0.021932424934107075 1108 5 0.06146114819727444 1108 6 -0.015389328684491943 1108 7 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-0.6412414639921629 2316 100-101 -0.1652043012977913 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 13.0 5 36.0 6 67.0 7 195.0 8 458.0 9 823.0 10 1549.0 11 2529.0 12 3768.0 13 5488.0 14 6674.0 15 9055.0 16 11477.0 17 16219.0 18 16081.0 19 11181.0 20 10363.0 21 10977.0 22 11490.0 23 12735.0 24 14408.0 25 17223.0 26 20577.0 27 24364.0 28 29932.0 29 36653.0 30 45092.0 31 55059.0 32 69201.0 33 90312.0 34 125788.0 35 184795.0 36 302726.0 37 524436.0 38 487716.0 39 48545.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.647542473632356 19.044647758795577 14.0278419068325 23.279967860739568 2 15.756188250532718 20.695873895784224 39.45281168832639 24.095126165356668 3 20.291302368869776 24.07972771924104 29.32067943379008 26.308290478099106 4 13.264583800381791 16.319137324701146 36.36827648362896 34.0480023912881 5 13.919425366222601 37.29222854011409 33.91630038745208 14.872045706211235 6 32.55793099231663 35.44799288048315 17.23851513689898 14.755560990301245 7 29.189792641807234 32.159835870680226 20.364444987919015 18.285926499593526 8 26.265038960333147 35.027705880621276 19.48283865825187 19.2244165007937 9 26.06920695738027 17.310163200884052 20.407922953421963 36.21270688831371 10-11 25.39405302953105 26.39825816400704 26.866710597527643 21.34097820893427 12-13 26.29130689782452 23.614536133133154 27.601374990659032 22.4927819783833 14-15 22.69621621034077 25.283333589976074 26.108334337259365 25.91211586242379 16-17 22.319372709436532 28.511434927616836 26.67755750525822 22.491634857688414 18-19 22.14091988115109 27.34657192992706 28.689158829871285 21.823349359050567 20-21 22.922715651841134 26.27196826108518 28.59708833499772 22.20822775207596 22-23 22.50387791749603 26.275749938292993 28.61497770622029 22.605394437990682 24-25 22.670825175509275 26.364049324208345 28.726161151049162 22.23896434923322 26-27 22.39950253420417 26.370012377767843 28.99418521180566 22.236299876222322 28-29 22.044709841192205 27.072823327740075 28.78524375966649 22.09722307140124 30-31 22.32770985897759 26.443660105971745 28.582932470752244 22.645697564298416 32-33 22.171339415719547 26.590172621109907 28.738369078108615 22.500118885061923 34-35 22.853547106133828 26.5531264770314 28.439174558699538 22.154151858135233 36-37 22.769778860206344 26.19745925263502 28.720380070146444 22.312381817012188 38-39 22.258426324978053 26.731809892070203 28.756524212858984 22.253239570092763 40-41 22.463617621353297 26.662722887860717 28.546903978875722 22.326755511910264 42-43 22.28271799312974 26.735547446842432 28.103462488948157 22.878272071079675 44-45 22.505217514180586 26.599924454742595 28.17371604766054 22.721141983416278 46-47 22.785584933569417 27.03865498873066 27.80718497867849 22.368575099021438 48-49 22.75498188405797 26.902626811594203 27.823052536231884 22.519338768115944 50-51 22.359487385380568 27.1634888101907 28.016336856636066 22.460686947792667 52-53 22.81222465351774 27.291794185135192 27.396481045066075 22.499500116280995 54-55 22.285967260186183 26.931293492118485 27.707035504740684 23.075703742954644 56-57 22.588048541336903 27.117139149869907 27.613354075946038 22.68145823284715 58-59 22.958436475770405 26.722132406242196 27.55957615110583 22.75985496688157 60-61 22.544522139454237 26.930583600343876 28.123049509506842 22.401844750695044 62-63 22.32157233249898 27.02428932048465 28.04822400606548 22.60591434095089 64-65 22.126470359118176 27.272546110353247 27.700610154875715 22.900373375652862 66-67 22.25331489060272 27.091610369235063 27.4541241605206 23.200950579641617 68-69 22.215468773742593 26.961190493066905 27.738050944200786 23.08528978898971 70-71 22.514966507224706 26.930994760675148 27.532530937876963 23.02150779422318 72-73 22.482523895897447 26.723527693425996 27.798973285960464 22.994975124716092 74-75 22.170569493413765 27.458010928367788 27.318043582131462 23.053375996086984 76-77 22.28982213021635 26.878267062023625 27.470213199554657 23.36169760820537 78-79 22.37333410024033 26.991940909513957 27.545223546973546 23.089501443272166 80-81 22.438726683530085 27.051453054550972 27.46181386680314 23.0480063951158 82-83 22.459422591102726 26.979889510969645 27.530840098876723 23.029847799050906 84-85 22.273920240578075 26.990736023079553 27.462200211502186 23.273143524840183 86-87 22.227768706178338 27.0420109888391 27.426577285153282 23.303643019829284 88-89 22.16144678186616 26.949365681287556 27.20694007126778 23.6822474655785 90-91 22.208981119910813 26.767469294194434 27.362364083886348 23.661185502008404 92-93 22.409902106643298 26.778713006931564 27.3953981290888 23.41598675733634 94-95 22.188209772125642 26.62110538198337 27.496190016825068 23.694494829065928 96-97 21.958320029528785 26.49436258358752 27.793764988009595 23.753552398874103 98-99 22.034627554623988 26.71727548441869 27.155752890139706 24.092344070817624 100-101 22.0480924784284 25.876247058418585 27.865652836327605 24.210007626825412 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 411.0 1 293.0 2 267.5 3 359.5 4 316.5 5 313.5 6 513.0 7 985.5 8 1584.0 9 1901.5 10 1787.0 11 1654.0 12 1752.5 13 1949.0 14 2158.5 15 2327.5 16 2467.0 17 2602.5 18 2830.0 19 3109.5 20 3457.0 21 3977.5 22 4792.0 23 5907.5 24 7205.5 25 8901.0 26 11337.5 27 14479.5 28 17317.0 29 20123.5 30 23602.0 31 27666.5 32 32330.5 33 35780.0 34 39263.5 35 43004.0 36 47159.5 37 52100.5 38 55583.5 39 58071.5 40 60900.5 41 64951.5 42 68932.0 43 72411.5 44 76029.0 45 80256.0 46 82377.5 47 88343.0 48 103418.5 49 103260.5 50 90261.0 51 83662.5 52 79835.5 53 78336.5 54 76602.5 55 70808.5 56 62763.5 57 56020.5 58 50392.0 59 44145.0 60 36890.0 61 30051.0 62 24699.5 63 19576.5 64 14725.0 65 11039.5 66 8297.0 67 6751.5 68 5245.5 69 3732.0 70 2777.0 71 2148.5 72 1730.0 73 1474.0 74 1222.5 75 973.0 76 654.0 77 347.0 78 197.0 79 138.0 80 86.0 81 45.5 82 25.5 83 17.0 84 10.0 85 7.5 86 4.5 87 2.0 88 1.0 89 0.5 90 0.0 91 1.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34379295430511114 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.699223057814371E-4 16-17 0.0019474505381530467 18-19 6.793432109836211E-5 20-21 0.0 22-23 0.0 24-25 5.434745687868969E-4 26-27 0.0010869491375737938 28-29 0.0 30-31 0.0030796892231257484 32-33 0.0 34-35 0.011345031623426472 36-37 0.028668283503508807 38-39 0.021240797730087885 40-41 0.014424720846552221 42-43 0.01082420182833903 44-45 0.0030343996757268405 46-47 0.0015171998378634202 48-49 6.79343210983621E-4 50-51 6.793432109836211E-5 52-53 6.793432109836211E-5 54-55 0.0 56-57 0.0 58-59 0.004778047250584802 60-61 0.010733622733541212 62-63 0.005004494987579341 64-65 0.0023097669173443116 66-67 0.004483665192491899 68-69 0.004053414492202272 70-71 6.793432109836211E-5 72-73 0.0 74-75 0.0 76-77 0.2162122992823871 78-79 5.208297950874428E-4 80-81 0.0036231637919126457 82-83 5.88764116185805E-4 84-85 0.0 86-87 0.10930632264726461 88-89 0.4005634019696424 90-91 0.3910525970158717 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0015171998378634202 100-101 8.605014005792533E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2208015.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.51122083518487 #Duplication Level Percentage of deduplicated Percentage of total 1 78.10087896677595 44.91676897680297 2 12.64851564914016 14.54863153469983 3 3.932651854611676 6.785148278354145 4 1.7506778060928523 4.02734471669852 5 0.9304774635184323 2.675644744328562 6 0.5802688246445175 2.0023181110742407 7 0.3801648404492012 1.5304620864992782 8 0.26598598993611045 1.2237743205024727 9 0.2035417208204779 1.0535339569752047 >10 1.09290729741539 11.362638232170617 >50 0.07499290648059992 2.9362098099448617 >100 0.03522737282432908 3.7465195521524484 >500 0.0024465643830302967 0.9951921730659478 >1k 0.0010259786122384408 1.27755514436221 >5k 2.367642951319479E-4 0.9182583623687025 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8719 0.39487956377107947 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6217 0.2815651161790114 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5295 0.2398081534772182 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3907 0.17694626168753383 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3305 0.14968195415339117 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2966 0.13432879758516134 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2732 0.12373104349381685 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2316 0.1048905917758711 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.528954739890807E-5 0.0 10-11 0.0 0.0 0.0 4.528954739890807E-5 4.528954739890807E-5 12-13 0.0 0.0 0.0 4.528954739890807E-5 6.793432109836211E-5 14-15 0.0 0.0 0.0 4.528954739890807E-5 9.057909479781614E-5 16-17 0.0 0.0 0.0 4.528954739890807E-5 1.1322386849727018E-4 18-19 0.0 0.0 0.0 4.528954739890807E-5 2.2644773699454036E-4 20-21 0.0 4.528954739890807E-5 0.0 4.528954739890807E-5 2.7173728439344844E-4 22-23 0.0 4.528954739890807E-5 0.0 9.057909479781614E-5 2.7173728439344844E-4 24-25 0.0 4.528954739890807E-5 0.0 2.490925106939944E-4 2.943820580929025E-4 26-27 0.0 4.528954739890807E-5 0.0 6.56698437284167E-4 3.170268317923565E-4 28-29 0.0 4.528954739890807E-5 0.0 0.0023777012384426735 3.170268317923565E-4 30-31 0.0 4.528954739890807E-5 0.0 0.008174763305502906 3.170268317923565E-4 32-33 0.0 9.057909479781614E-5 0.0 0.018432845791355585 3.170268317923565E-4 34-35 0.0 9.057909479781614E-5 0.0 0.03580138721883683 3.6231637919126455E-4 36-37 0.0 9.057909479781614E-5 0.0 0.06677943763968994 3.6231637919126455E-4 38-39 0.0 9.057909479781614E-5 0.0 0.11157080001721004 4.076059265901726E-4 40-41 0.0 9.057909479781614E-5 0.0 0.16150252602450615 4.076059265901726E-4 42-43 0.0 9.057909479781614E-5 0.0 0.21279293845376956 4.076059265901726E-4 44-45 0.0 9.057909479781614E-5 0.0 0.2770361614391207 4.3025070028962666E-4 46-47 0.0 9.057909479781614E-5 0.0 0.3589875974574448 4.528954739890807E-4 48-49 0.0 9.057909479781614E-5 0.0 0.4449245136468729 4.528954739890807E-4 50-51 0.0 9.057909479781614E-5 0.0 0.5389909035944049 5.434745687868969E-4 52-53 0.0 9.057909479781614E-5 0.0 0.634800941116795 5.887641161858049E-4 54-55 0.0 9.057909479781614E-5 0.0 0.7384460703391961 5.887641161858049E-4 56-57 0.0 9.057909479781614E-5 0.0 0.8694007966431387 6.114088898852589E-4 58-59 0.0 9.057909479781614E-5 0.0 1.0154369422309177 6.34053663584713E-4 60-61 0.0 9.057909479781614E-5 0.0 1.1751052415857681 6.34053663584713E-4 62-63 0.0 9.057909479781614E-5 0.0 1.3383967047325314 6.79343210983621E-4 64-65 0.0 1.3586864219672422E-4 0.0 1.5058321614662944 7.246327583825291E-4 66-67 0.0 1.3586864219672422E-4 0.0 1.707393292165135 7.472775320819831E-4 68-69 0.0 1.3586864219672422E-4 0.0 1.9415402522174894 7.699223057814372E-4 70-71 0.0 1.3586864219672422E-4 0.0 2.198467854611495 7.699223057814372E-4 72-73 0.0 1.3586864219672422E-4 0.0 2.4594262267240032 8.152118531803453E-4 74-75 0.0 1.3586864219672422E-4 0.0 2.732182525933927 8.605014005792533E-4 76-77 0.0 1.3586864219672422E-4 0.0 3.0299386553080483 8.605014005792533E-4 78-79 0.0 1.3586864219672422E-4 0.0 3.381408187897274 8.605014005792533E-4 80-81 0.0 1.3586864219672422E-4 0.0 3.763538744075561 8.605014005792533E-4 82-83 4.528954739890807E-5 1.3586864219672422E-4 0.0 4.144582351116274 8.605014005792533E-4 84-85 4.528954739890807E-5 1.3586864219672422E-4 0.0 4.539303401471457 8.605014005792533E-4 86-87 4.528954739890807E-5 1.3586864219672422E-4 0.0 4.955582276388521 8.831461742787074E-4 88-89 4.528954739890807E-5 1.3586864219672422E-4 0.0 5.4204794804383125 9.284357216776155E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8670 0.0 26.416143 1 TACATGG 8605 0.0 25.804272 2 ACATGGG 8510 0.0 25.088783 3 TACCTGG 1985 0.0 22.228954 2 CATGGGA 4375 0.0 21.038769 4 GTGTAGC 1100 0.0 20.777435 1 GAGTACT 7095 0.0 20.23015 12-13 CATGGGG 4470 0.0 19.105642 4 GTACGAT 125 0.006072868 18.97827 3 GTACCTG 2400 0.0 18.64919 1 GTACTTT 7580 0.0 18.622755 14-15 TAGGACG 155 9.702626E-4 18.366068 4 CGTGTCG 130 0.0076153637 18.248337 7 GAGTACA 7400 0.0 17.17999 1 GTGTAGG 1015 0.0 16.888063 1 AGTACAT 6605 0.0 16.808914 2 AGAGTAC 11965 0.0 16.715178 10-11 AGTACTT 7540 0.0 16.644873 12-13 GTAGCCC 1135 0.0 16.302917 3 ACTTTTT 8800 0.0 16.228945 16-17 >>END_MODULE