FastQCFastQC Report
Fri 27 May 2016
SRR939118_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939118_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2171509
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT78730.36255893942875667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57590.26520728212501077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46720.2151499256968311No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT41180.18963771276103392No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31660.14579723132623443No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29970.13801462485304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28440.13096883319387578No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT21750.10016076378223622No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG88050.028.0435331
TACATGG87700.027.003882
ACATGGG86800.025.5377043
TACCTGG19750.022.3034292
CATGGGG46150.021.1422544
GAGTACA68300.020.579261
GAGTACT63500.020.5748112-13
ATTCGCG1850.020.56506380-81
GTACCTG22100.019.9824811
CATGGGA44650.019.5187534
AGTACAT64600.019.2098752
GTACTTT68300.019.1988214-15
AGTACTT68100.018.97649612-13
CGCGATC2250.018.94068122-23
AGAGTAC107000.018.8233210-11
CCGCGAT2400.018.74338222-23
CGCGGAA2101.8189894E-1218.11621382-83
TCGCGCT2500.018.07657460-61
GTATCAA149150.018.0517181
GTACACG3208.314601E-917.806971
Produced by FastQC (version 0.11.5)