##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939118_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2171509 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.139479044295925 33.0 31.0 34.0 30.0 34.0 2 32.23020305234747 34.0 31.0 34.0 30.0 34.0 3 32.298437169728516 34.0 31.0 34.0 30.0 34.0 4 35.72518511320929 37.0 35.0 37.0 35.0 37.0 5 35.768517192422415 37.0 35.0 37.0 35.0 37.0 6 35.853031693628715 37.0 37.0 37.0 35.0 37.0 7 35.78300711624958 37.0 36.0 37.0 35.0 37.0 8 35.78044760578934 37.0 36.0 37.0 35.0 37.0 9 37.46816384366816 39.0 38.0 39.0 35.0 39.0 10-11 37.374963907586846 39.0 38.0 39.0 35.0 39.0 12-13 37.279194099586974 39.0 37.0 39.0 34.0 39.0 14-15 38.57981500422056 40.0 38.0 41.0 34.0 41.0 16-17 38.497909287965186 40.0 38.0 41.0 34.0 41.0 18-19 38.384160277484455 40.0 38.0 41.0 34.0 41.0 20-21 38.36604407349912 40.0 38.0 41.0 34.0 41.0 22-23 38.41051637363695 40.0 38.0 41.0 34.0 41.0 24-25 38.357199993184466 40.0 38.0 41.0 34.0 41.0 26-27 38.24092301712772 40.0 38.0 41.0 34.0 41.0 28-29 38.109917573447774 40.0 38.0 41.0 33.5 41.0 30-31 37.91806757420761 40.0 38.0 41.0 33.0 41.0 32-33 37.72891178438588 40.0 38.0 41.0 33.0 41.0 34-35 37.42413156012708 40.0 37.0 41.0 31.5 41.0 36-37 37.16507737246311 40.0 37.0 41.0 31.0 41.0 38-39 36.985586750964416 40.0 37.0 41.0 31.0 41.0 40-41 36.82005531637216 40.0 37.0 41.0 30.0 41.0 42-43 36.619090917882446 40.0 36.0 41.0 30.0 41.0 44-45 36.35696996880971 40.0 36.0 41.0 30.0 41.0 46-47 36.2400013078463 40.0 36.0 41.0 29.5 41.0 48-49 36.12734946067458 40.0 36.0 41.0 29.0 41.0 50-51 35.012113465797285 38.5 34.5 40.0 26.5 40.5 52-53 35.16087683725925 38.5 35.0 39.5 27.5 40.5 54-55 35.697473968562875 39.0 35.0 41.0 28.0 41.0 56-57 35.60268228222863 39.0 35.0 41.0 27.5 41.0 58-59 35.40145723549845 39.0 35.0 41.0 27.0 41.0 60-61 35.09919484561197 38.5 35.0 41.0 26.0 41.0 62-63 34.73234465065538 38.0 34.5 40.0 26.0 41.0 64-65 34.363399829335265 37.5 34.0 40.0 25.5 41.0 66-67 33.97580254099799 37.0 34.0 40.0 24.5 41.0 68-69 33.55741399183701 36.5 34.0 39.0 23.0 41.0 70-71 33.11766241816174 36.0 33.0 39.0 22.0 41.0 72-73 32.672849847732614 35.0 33.0 38.5 21.5 40.0 74-75 32.23220649787774 35.0 32.5 37.5 20.0 39.5 76-77 31.798800511533685 35.0 32.0 37.0 18.5 39.0 78-79 31.359739932001204 35.0 32.0 36.5 17.0 39.0 80-81 30.90183001774342 35.0 31.0 36.0 11.5 37.5 82-83 30.504830972379118 35.0 31.0 35.5 7.0 37.0 84-85 30.115936659714514 34.5 31.0 35.0 4.0 37.0 86-87 29.718715648887482 34.0 30.5 35.0 2.0 36.0 88-89 29.300146349842436 34.0 30.0 35.0 2.0 36.0 90-91 28.89088969928285 34.0 29.0 35.0 2.0 36.0 92-93 28.47951171282274 34.0 29.0 35.0 2.0 35.0 94-95 27.997906755164266 34.0 29.0 35.0 2.0 35.0 96-97 27.39920810827862 34.0 27.0 35.0 2.0 35.0 98-99 26.69987598485661 34.0 25.5 35.0 2.0 35.0 100-101 23.755627998778728 30.0 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05138820210881079 1101 2 0.16707385029569366 1101 3 0.20362321240055792 1101 4 0.24970830014556356 1101 5 0.03994445426720006 1101 6 0.09221835287910807 1101 7 0.10096322467550323 1101 8 0.17127768823289813 1101 9 0.21128097458414885 1101 10-11 0.22656162662320867 1101 12-13 0.22025983623697698 1101 14-15 0.27179859714956933 1101 16-17 0.27599583086115587 1101 18-19 0.42753684686243076 1101 20-21 0.3613037904003207 1101 22-23 0.28690563750487286 1101 24-25 0.38121309568859374 1101 26-27 0.2861264235542933 1101 28-29 0.24969314702265422 1101 30-31 0.3152807738830532 1101 32-33 0.1654591327840791 1101 34-35 0.2209031213483712 1101 36-37 0.16630324775349692 1101 38-39 0.08496217374460713 1101 40-41 0.06899080776818778 1101 42-43 0.04332238090930218 1101 44-45 0.03665022466869772 1101 46-47 -0.03148082268295127 1101 48-49 -0.022090895771015084 1101 50-51 -0.09957438828448772 1101 52-53 -0.006679335418361632 1101 54-55 -0.013156643481899266 1101 56-57 -0.04415540428166054 1101 58-59 -0.15502811936362093 1101 60-61 -0.15384878838521132 1101 62-63 -0.13200003356137557 1101 64-65 -0.16651044360680345 1101 66-67 -0.15882236999513566 1101 68-69 -0.27828972690191023 1101 70-71 -0.21970103059587842 1101 72-73 -0.14530135546996092 1101 74-75 -0.1146952184723844 1101 76-77 -0.22446124303459314 1101 78-79 -0.34457243579377206 1101 80-81 -0.5345918985065232 1101 82-83 -0.524628154798048 1101 84-85 -0.4836561964568027 1101 86-87 -0.6189037430133837 1101 88-89 -0.7822532131170803 1101 90-91 -0.790480681979215 1101 92-93 -0.8745417007606804 1101 94-95 -1.0085383587463461 1101 96-97 -1.126647398272656 1101 98-99 -1.3513344880127285 1101 100-101 -1.3638775152320974 1102 1 0.19440403244242788 1102 2 0.23243484194308195 1102 3 0.24805598533363593 1102 4 0.1464315435163286 1102 5 0.19198514566542002 1102 6 0.20760503241115202 1102 7 0.28116890664551164 1102 8 0.27806717224463284 1102 9 0.34941387067372176 1102 10-11 0.3425266329599239 1102 12-13 0.32952041648688635 1102 14-15 0.3879008477884085 1102 16-17 0.4081208646131884 1102 18-19 0.37202921345174644 1102 20-21 0.40772227315477494 1102 22-23 0.43490866302894915 1102 24-25 0.5994290203881576 1102 26-27 0.5131736417436628 1102 28-29 0.430267655584629 1102 30-31 0.448668119790689 1102 32-33 0.41102426904167544 1102 34-35 0.32446750402014857 1102 36-37 0.33880745592654904 1102 38-39 0.3385226990408796 1102 40-41 0.33758014318711105 1102 42-43 0.34049584708542824 1102 44-45 0.22033690850535237 1102 46-47 0.45611212469294315 1102 48-49 0.42067180128356796 1102 50-51 0.28492698443805153 1102 52-53 0.4350063712164882 1102 54-55 0.3700557229285266 1102 56-57 0.45466809619156834 1102 58-59 0.4888350757396438 1102 60-61 0.3385082972501934 1102 62-63 0.25688491099025157 1102 64-65 0.35279418339896296 1102 66-67 0.3361021065059049 1102 68-69 0.22962488086729138 1102 70-71 0.17258329599301447 1102 72-73 0.1572138769704523 1102 74-75 0.14899776574424095 1102 76-77 0.06952345939684079 1102 78-79 -0.10465150640107268 1102 80-81 -0.05786948251484958 1102 82-83 -0.20072601138306112 1102 84-85 -0.2741600367815771 1102 86-87 -0.2184335345306252 1102 88-89 -0.3007673576657979 1102 90-91 -0.3509963348176548 1102 92-93 -0.37269589068107933 1102 94-95 -0.6179013830629074 1102 96-97 -0.5826060930486001 1102 98-99 -0.7933658210825669 1102 100-101 -1.0315995561079774 1103 1 0.19369143186919047 1103 2 0.2742413768711174 1103 3 0.3215835811988086 1103 4 0.28925056962525275 1103 5 0.2071752332282557 1103 6 0.29162852755921165 1103 7 0.28055503660971226 1103 8 0.3190068911318633 1103 9 0.3351012065648007 1103 10-11 0.25034603712793313 1103 12-13 0.35719937306556915 1103 14-15 0.3510629339565057 1103 16-17 0.41625389462890894 1103 18-19 0.29769518220502533 1103 20-21 0.3159994279963598 1103 22-23 0.341657534427398 1103 24-25 0.42269102294807936 1103 26-27 0.4849180770675403 1103 28-29 0.47063307824652156 1103 30-31 0.47395306482525257 1103 32-33 0.4128260333536673 1103 34-35 0.38593365522093137 1103 36-37 0.39870411796403715 1103 38-39 0.3722807912731483 1103 40-41 0.2887746194991294 1103 42-43 0.3901781142649483 1103 44-45 0.4326487697146888 1103 46-47 0.5570555316961219 1103 48-49 0.502356225847727 1103 50-51 0.6505230367396706 1103 52-53 0.6557749245339721 1103 54-55 0.4845989214824371 1103 56-57 0.6152964249473953 1103 58-59 0.6861450629726704 1103 60-61 0.7389846516944587 1103 62-63 0.41139684215412586 1103 64-65 0.42134676652164416 1103 66-67 0.3760439126056525 1103 68-69 0.5304271820775668 1103 70-71 0.47492516802191886 1103 72-73 0.3668702131046899 1103 74-75 0.393601720296644 1103 76-77 0.3194584549934021 1103 78-79 0.40230450873652046 1103 80-81 0.19760652650852606 1103 82-83 0.03293408467114389 1103 84-85 -0.048267422968120144 1103 86-87 -0.07761196590637454 1103 88-89 0.09959598703106565 1103 90-91 0.1169337835447557 1103 92-93 0.03455792078004194 1103 94-95 -0.0732885074040901 1103 96-97 -0.05300692271149643 1103 98-99 -0.14291149741938725 1103 100-101 -0.2275600900035606 1104 1 0.18036758975395628 1104 2 0.2712781611869062 1104 3 0.19282372894508626 1104 4 0.21556901062093203 1104 5 0.27993503871674363 1104 6 0.24668553731002874 1104 7 0.28820460765820144 1104 8 0.32421457264144493 1104 9 0.3543520114716401 1104 10-11 0.3661658676623958 1104 12-13 0.3674089424560236 1104 14-15 0.3611873427814274 1104 16-17 0.33345430945784216 1104 18-19 0.3956165469847903 1104 20-21 0.40667483763727574 1104 22-23 0.49673505711318455 1104 24-25 0.37474509313154414 1104 26-27 0.347255956460522 1104 28-29 0.3420056832218634 1104 30-31 0.47247663835920406 1104 32-33 0.2616783851271194 1104 34-35 0.38388116602143896 1104 36-37 0.41780439725349083 1104 38-39 0.14200967776354645 1104 40-41 0.27577095985335376 1104 42-43 0.4282361185742616 1104 44-45 0.2004637356013106 1104 46-47 0.3598491702417306 1104 48-49 0.3813188824747513 1104 50-51 0.35504855687675985 1104 52-53 0.3344740749742954 1104 54-55 0.3398242329562109 1104 56-57 0.4381624486451514 1104 58-59 0.4427827378474305 1104 60-61 0.4822885202692575 1104 62-63 0.4022598176084884 1104 64-65 0.4369696781945933 1104 66-67 0.38681519630353023 1104 68-69 0.22901524381512672 1104 70-71 0.3710720576418751 1104 72-73 0.24650405721541802 1104 74-75 0.2410430106559076 1104 76-77 0.10137128086542191 1104 78-79 0.10452365224708515 1104 80-81 0.1181739057871134 1104 82-83 0.09771324246636226 1104 84-85 0.025086912209520307 1104 86-87 -0.0886112301509705 1104 88-89 -0.05032633218474203 1104 90-91 -0.04483953842675348 1104 92-93 -0.004018353991256163 1104 94-95 -0.10816009829114037 1104 96-97 -0.001101491593107795 1104 98-99 -0.007688742091140455 1104 100-101 -0.3774724450346376 1105 1 0.09776179710505062 1105 2 0.1474788642335696 1105 3 0.17137954296988767 1105 4 0.14241654391463499 1105 5 0.23275370643275295 1105 6 0.18753267220546377 1105 7 0.1721282069313972 1105 8 0.16830556103248995 1105 9 0.18261478630781625 1105 10-11 0.2889874138445023 1105 12-13 0.20095667899468594 1105 14-15 0.2857300416178532 1105 16-17 0.21344482108522556 1105 18-19 0.33212717089554644 1105 20-21 0.27658865631040186 1105 22-23 0.26947358647727526 1105 24-25 0.2687408756761869 1105 26-27 0.3028775853115633 1105 28-29 0.35037797377222546 1105 30-31 0.30020585329558713 1105 32-33 0.34741299570569595 1105 34-35 0.29897208509221684 1105 36-37 0.34259731906151814 1105 38-39 0.26298565558519016 1105 40-41 0.2919464189841605 1105 42-43 0.19138177952496704 1105 44-45 0.21850323990728526 1105 46-47 0.2656350675101322 1105 48-49 0.3000245055853483 1105 50-51 0.26530793063901115 1105 52-53 0.3257443598496721 1105 54-55 0.37901079956461814 1105 56-57 0.31401886962171943 1105 58-59 0.4067905728414871 1105 60-61 0.4844974917549152 1105 62-63 0.39056453507170374 1105 64-65 0.19628661844438255 1105 66-67 0.3840170025474592 1105 68-69 0.2680001631781366 1105 70-71 0.20285960055794305 1105 72-73 0.34995759807662097 1105 74-75 0.2759198666802902 1105 76-77 0.21832758513140504 1105 78-79 0.23951765694591742 1105 80-81 0.28162813212010107 1105 82-83 0.19699661521432432 1105 84-85 0.021727971608743957 1105 86-87 0.06488600309420534 1105 88-89 0.03823791108146324 1105 90-91 0.06423061909518069 1105 92-93 0.009311366441909996 1105 94-95 -0.02954949806266427 1105 96-97 0.006777568506532106 1105 98-99 0.15763184868246327 1105 100-101 -0.06431543256806904 1106 1 0.21991380938403893 1106 2 0.26295982449296673 1106 3 0.33742495130604766 1106 4 0.30104671912941683 1106 5 0.3299180663594825 1106 6 0.21522684035052464 1106 7 0.2748008359061771 1106 8 0.2697090954497625 1106 9 0.28294191591714224 1106 10-11 0.3911583850229974 1106 12-13 0.26515005091026467 1106 14-15 0.3808722665864579 1106 16-17 0.3141805858531228 1106 18-19 0.4322139920240815 1106 20-21 0.38360234171227603 1106 22-23 0.3304532477419855 1106 24-25 0.4278339492548753 1106 26-27 0.38824689625744924 1106 28-29 0.47004273790446405 1106 30-31 0.4597053472081427 1106 32-33 0.5941023864738639 1106 34-35 0.5161899108873911 1106 36-37 0.5552957950406352 1106 38-39 0.6160951587335646 1106 40-41 0.5881072418358428 1106 42-43 0.6288118741201316 1106 44-45 0.49930203858635736 1106 46-47 0.5643207905285834 1106 48-49 0.5251747260711497 1106 50-51 0.6550916087098102 1106 52-53 0.5479831932571955 1106 54-55 0.5625832232251398 1106 56-57 0.6613277468606285 1106 58-59 0.6770855653443064 1106 60-61 0.6868150843290479 1106 62-63 0.4631210850835501 1106 64-65 0.47427148233233396 1106 66-67 0.5415021133883613 1106 68-69 0.6191506538725449 1106 70-71 0.4805233148632766 1106 72-73 0.4748415966325652 1106 74-75 0.5500963394833462 1106 76-77 0.6792314993911077 1106 78-79 0.6321440625290471 1106 80-81 0.5313197177795566 1106 82-83 0.5475247401658763 1106 84-85 0.6721533286938559 1106 86-87 0.5820376056306209 1106 88-89 0.6254754266682916 1106 90-91 0.6269090509106938 1106 92-93 0.602174536786297 1106 94-95 0.9145206410061775 1106 96-97 0.9902782318465349 1106 98-99 0.9412213264932419 1106 100-101 0.8275147435326424 1107 1 -0.12493317449246888 1107 2 -0.027277916742590946 1107 3 -0.03787608130740239 1107 4 -0.06561883991162176 1107 5 0.016849333004799405 1107 6 -0.06069778548999949 1107 7 0.008545546110070745 1107 8 -0.02625271361867476 1107 9 0.011046219372630617 1107 10-11 0.06941953533698353 1107 12-13 -0.04053352967103763 1107 14-15 -0.10966707875024184 1107 16-17 -0.16489931189653362 1107 18-19 -0.06236088122936678 1107 20-21 -0.027407951900428884 1107 22-23 0.07740674702591832 1107 24-25 0.06598573685658948 1107 26-27 -0.009975388180336608 1107 28-29 0.020466687504203662 1107 30-31 -0.009005642865410834 1107 32-33 -0.014792059396356194 1107 34-35 0.011844012130509896 1107 36-37 0.1485655318098722 1107 38-39 0.027830240135799045 1107 40-41 0.07227396918624862 1107 42-43 0.09075527162269736 1107 44-45 0.04054117943650937 1107 46-47 0.17182021727340668 1107 48-49 0.14654822426631142 1107 50-51 0.13435756399803012 1107 52-53 0.21506109539541285 1107 54-55 0.0701374656551721 1107 56-57 0.10080696485808716 1107 58-59 0.10726414523156791 1107 60-61 -0.017361795470705488 1107 62-63 0.13034686702599885 1107 64-65 0.1203244112833346 1107 66-67 0.08134847393087341 1107 68-69 0.1437222678786796 1107 70-71 0.15782931482415563 1107 72-73 0.31993873893488 1107 74-75 0.1997464594014957 1107 76-77 0.1853219085079516 1107 78-79 0.1617340454456908 1107 80-81 0.12910715311012666 1107 82-83 0.09497400796642097 1107 84-85 0.18316159558732537 1107 86-87 0.07722202647556742 1107 88-89 -0.01886091822841962 1107 90-91 -0.05100343299632826 1107 92-93 0.03659709030641167 1107 94-95 0.16159786442911184 1107 96-97 0.28713816033162587 1107 98-99 0.0472950976592017 1107 100-101 0.19433572365877794 1108 1 0.05574351830664881 1108 2 -0.027713482999381256 1108 3 -0.018817877446785758 1108 4 0.1863991537022116 1108 5 0.19556873149193166 1108 6 0.19663420825143163 1108 7 0.09421325908429878 1108 8 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Count 2 7177.0 3 1923.0 4 1979.0 5 2222.0 6 2438.0 7 2877.0 8 3480.0 9 4397.0 10 5107.0 11 6296.0 12 8374.0 13 13180.0 14 11131.0 15 10695.0 16 12783.0 17 17506.0 18 14989.0 19 11270.0 20 11266.0 21 11634.0 22 12604.0 23 13872.0 24 15825.0 25 17806.0 26 20436.0 27 23913.0 28 27927.0 29 33783.0 30 40474.0 31 49951.0 32 63425.0 33 82797.0 34 115236.0 35 172150.0 36 283611.0 37 470979.0 38 479163.0 39 86801.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.4966629421307 18.44153826879808 13.642388147192058 23.419410641879164 2 15.513058712339504 20.20239275258754 40.10442359558332 24.180124939489637 3 20.30527249029858 23.94928015645826 29.079770384041037 26.665676969202124 4 12.941001255096236 15.891814577744498 36.704971232382775 34.462212934776495 5 14.017200977168958 37.118587328711854 33.91530649039016 14.94890520372903 6 32.674345032449416 35.062693750625 17.47420383383725 14.788757383088333 7 29.19637384262993 32.04844708474092 20.482212564349545 18.272966508279602 8 26.258674014663736 34.68458317030311 19.731104969345708 19.325637845687453 9 26.23411301569043 17.25214276694604 20.51991420636742 35.99383001099611 10-11 25.433193826168527 26.179241859246545 27.04983477352994 21.33772954105499 12-13 26.330383237476536 23.541322503538893 27.613118436512952 22.515175822471623 14-15 22.833281104546668 25.1192429283759 26.156783136860927 25.890692830216505 16-17 22.45458219676493 28.3305198512933 26.689736758546097 22.525161193395668 18-19 22.353287116615597 27.27291791443585 28.583210947292155 21.790584021656397 20-21 23.11012460980222 26.1987073985601 28.415570026358083 22.275597965279598 22-23 22.61819681935352 26.177289755488236 28.49007445169513 22.714438973463118 24-25 22.78068252223812 26.277332052397885 28.562962833203027 22.379022592160972 26-27 22.469664183129588 26.297264728579606 28.821861149962796 22.411209938328007 28-29 22.116383897178252 27.00864062057398 28.70076310999649 22.174212372251276 30-31 22.415913360755326 26.437880148307393 28.437974177109705 22.70823231382758 32-33 22.231614714635057 26.551480089593316 28.55276466913089 22.664140526640736 34-35 22.97449093694956 26.49047765149905 28.309210173683052 22.22582123786834 36-37 22.858448406049785 26.230283086521318 28.540803727388425 22.37046478004047 38-39 22.353626225914493 26.73840553816188 28.530411566596992 22.377556669326633 40-41 22.5436531183972 26.629721047619313 28.426886637476667 22.399739196506825 42-43 22.32699875672208 26.665997043164797 28.019161106785816 22.98784309332731 44-45 22.52318290982552 26.59417223865065 28.040349743451102 22.842295108072726 46-47 22.882437870293607 26.926475700592377 27.73495923443222 22.456127194681798 48-49 22.860454172685802 26.8132337681306 27.754012440480892 22.57229961870271 50-51 22.401938397817357 27.150045517998993 27.970457395129795 22.477558689053854 52-53 22.85229536067143 27.262498712487083 27.367004255447192 22.518201671394298 54-55 22.30612040371422 26.85520766767247 27.74043583490276 23.098236093710543 56-57 22.62868201700396 27.11108015704509 27.576254981702135 22.683982844248806 58-59 22.98178377209066 26.66942757756675 27.60179027334175 22.74699837700084 60-61 22.675548873140198 26.852865253878473 28.13836947163929 22.333216401342042 62-63 22.388464162369264 26.970997951500642 28.076706257411114 22.56383162871898 64-65 22.18033782613889 27.177361037681603 27.728853922555352 22.913447213624156 66-67 22.28124736667139 27.051889653460897 27.48343144363357 23.18343153623414 68-69 22.315864004625208 26.909281696601088 27.76108772960779 23.013766569165913 70-71 22.51105423788618 26.870677108825376 27.637979439511128 22.980289213777315 72-73 22.544521993212985 26.666132681315613 27.890957722018495 22.89838760345291 74-75 22.199246684276446 27.461506585146207 27.430415527471485 22.908831203105866 76-77 22.429036998222728 26.902883281678502 27.382924904881584 23.285154815217187 78-79 22.547603836674988 26.997523927156582 27.5558146062151 22.899057629953333 80-81 22.623975141682738 27.101475870026064 27.388208266158752 22.886340722132452 82-83 22.647661234890197 26.99162437194361 27.542161648001446 22.818552745164748 84-85 22.481321588043965 27.024749112797124 27.488073239508324 23.005856059650583 86-87 22.495064288638503 27.0556824148895 27.473265234286316 22.97598806218568 88-89 22.437854505839084 26.982935578563016 27.20012627796845 23.379083637629446 90-91 22.51445770470309 26.872742104919578 27.393501891967347 23.21929829840999 92-93 22.802364689671524 26.8250966766924 27.397841257611898 22.97469737602418 94-95 22.554620144641323 26.688085922715203 27.557526536597045 23.19976739604643 96-97 22.402585151398913 26.552203173009904 27.845033517009217 23.20017815858197 98-99 22.49963481420898 26.830888768459243 27.15491447476097 23.514561942570804 100-101 22.70779904132362 25.960221248677023 27.777915356805032 23.554064353194317 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1862.0 1 1070.5 2 362.5 3 433.0 4 371.5 5 393.5 6 617.0 7 1101.5 8 1724.5 9 2058.5 10 2034.0 11 2007.0 12 2189.0 13 2462.5 14 2728.0 15 2960.5 16 3168.0 17 3323.5 18 3364.0 19 3445.5 20 3714.0 21 4173.5 22 4864.5 23 5856.0 24 7078.0 25 8725.5 26 11070.0 27 13935.0 28 16592.0 29 19373.0 30 22792.0 31 26791.0 32 31397.0 33 34766.5 34 38027.0 35 41693.5 36 46149.0 37 51171.5 38 54677.0 39 56983.0 40 59345.0 41 63582.0 42 67685.0 43 70857.0 44 74429.0 45 78853.0 46 81339.0 47 86894.0 48 100832.5 49 100835.0 50 88448.0 51 81957.5 52 77895.0 53 76251.5 54 74513.0 55 68812.5 56 61122.5 57 54751.0 58 49480.5 59 43180.5 60 36219.5 61 29962.0 62 24674.5 63 19587.0 64 14750.0 65 11160.5 66 8549.5 67 6869.5 68 5430.5 69 3958.5 70 2972.5 71 2298.0 72 1887.0 73 1522.0 74 1129.0 75 878.5 76 651.5 77 420.5 78 262.5 79 187.5 80 137.0 81 95.0 82 61.0 83 44.0 84 25.5 85 18.5 86 17.5 87 15.0 88 15.5 89 16.5 90 14.5 91 9.0 92 5.5 93 5.0 94 5.5 95 4.0 96 4.5 97 4.5 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38572255514483245 2 0.11323922673127304 3 0.11494311098871798 4 0.126686097087325 5 0.12871233782590816 6 0.06981320362936556 7 0.07916154158237428 8 0.0767208425108991 9 0.07603007862274574 10-11 0.07407291427297791 12-13 0.07794119204663669 14-15 0.07225390270084076 16-17 0.07368148140302436 18-19 0.08305284481897152 20-21 0.08178644435735703 22-23 0.09124990962505797 24-25 0.10412114340764879 26-27 0.10550267118395548 28-29 0.0995851272087751 30-31 0.09030586564458172 32-33 0.0887631596277059 34-35 0.3378756431587435 36-37 0.5294935457324838 38-39 0.5102903096418204 40-41 0.4833044670779629 42-43 0.5107508189005895 44-45 0.5202373096312287 46-47 0.5171518975974772 48-49 0.5242207147195798 50-51 0.5238062563866878 52-53 0.522056321203366 54-55 0.5166223119498929 56-57 0.48818586522091323 58-59 0.4783079416203202 60-61 0.4275137703781104 62-63 0.502093244835734 64-65 0.5278817633267926 66-67 0.5385655781302311 68-69 0.554384071168943 70-71 0.5493875457113003 72-73 0.5364702610028327 74-75 0.5399010549806609 76-77 0.5146421221371866 78-79 0.4894062147566508 80-81 0.4965901591934456 82-83 0.5099679531606823 84-85 0.503175441593841 86-87 0.5056161406653161 88-89 0.5155631406547244 90-91 0.5336381290614038 92-93 0.5403155133135529 94-95 0.5348584785971414 96-97 0.535825548040556 98-99 0.5364702610028327 100-101 0.5127540341762341 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2171509.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.47048028509365 #Duplication Level Percentage of deduplicated Percentage of total 1 79.53160488504895 47.29782740358162 2 11.972246742178143 14.23990527697964 3 3.6701889920707935 6.548037062865417 4 1.584603122240151 3.76948434963523 5 0.8240663050945183 2.4503809475366762 6 0.5040825908420369 1.798682026843817 7 0.36640687565159624 1.5253275012332712 8 0.24204442738711943 1.1515598677633976 9 0.19370286206956883 1.0367642015887057 >10 1.009022723159015 10.875991200237108 >50 0.0652308961149029 2.645754522999336 >100 0.033230898234466046 3.598390531688826 >500 0.0022498199424038656 0.9214059697556558 >1k 0.0011636999702088443 1.511546862006598 >5k 1.5515999602784588E-4 0.6289422752845963 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7873 0.36255893942875667 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5759 0.26520728212501077 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4672 0.2151499256968311 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4118 0.18963771276103392 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3166 0.14579723132623443 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2997 0.13801462485304 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2844 0.13096883319387578 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2175 0.10016076378223622 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.1512731469222555E-4 14-15 0.0 2.302546293844511E-5 0.0 2.302546293844511E-5 2.0722916644600598E-4 16-17 0.0 4.605092587689022E-5 0.0 4.605092587689022E-5 2.302546293844511E-4 18-19 0.0 4.605092587689022E-5 0.0 9.210185175378044E-5 2.302546293844511E-4 20-21 0.0 4.605092587689022E-5 0.0 1.3815277763067066E-4 2.302546293844511E-4 22-23 0.0 4.605092587689022E-5 0.0 3.453819440766766E-4 2.302546293844511E-4 24-25 0.0 4.605092587689022E-5 0.0 5.756365734611278E-4 2.302546293844511E-4 26-27 0.0 4.605092587689022E-5 0.0 9.440439804762494E-4 2.302546293844511E-4 28-29 0.0 4.605092587689022E-5 0.0 0.0023946481455982913 2.302546293844511E-4 30-31 0.0 4.605092587689022E-5 0.0 0.006861587955656643 2.302546293844511E-4 32-33 0.0 4.605092587689022E-5 0.0 0.01708489350032627 2.763055552613413E-4 34-35 0.0 4.605092587689022E-5 0.0 0.034607270796483 2.763055552613413E-4 36-37 0.0 4.605092587689022E-5 0.0 0.06638240965153724 2.763055552613413E-4 38-39 0.0 4.605092587689022E-5 0.0 0.11211097904728923 2.763055552613413E-4 40-41 0.0 4.605092587689022E-5 0.0 0.16041840029214707 2.763055552613413E-4 42-43 0.0 9.210185175378044E-5 0.0 0.21558740949266156 2.763055552613413E-4 44-45 0.0 9.210185175378044E-5 0.0 0.2798745020168003 2.763055552613413E-4 46-47 0.0 9.210185175378044E-5 0.0 0.3595426037838204 2.763055552613413E-4 48-49 0.0 9.210185175378044E-5 0.0 0.44252637221397656 2.763055552613413E-4 50-51 0.0 1.1512731469222555E-4 0.0 0.5354341151706026 3.6840740701512176E-4 52-53 0.0 1.8420370350756088E-4 0.0 0.627535966924383 3.6840740701512176E-4 54-55 0.0 2.302546293844511E-4 0.0 0.7378049089365966 3.6840740701512176E-4 56-57 0.0 2.302546293844511E-4 0.0 0.868543487501088 4.374837958304571E-4 58-59 0.0 2.302546293844511E-4 0.0 1.0121072489222933 4.835347217073473E-4 60-61 0.0 2.302546293844511E-4 0.0 1.1775221746720828 5.065601846457924E-4 62-63 0.0 2.302546293844511E-4 0.0 1.3415785981085042 5.065601846457924E-4 64-65 0.0 2.302546293844511E-4 0.0 1.5109999544095833 5.065601846457924E-4 66-67 0.0 2.302546293844511E-4 0.0 1.712679984287424 5.295856475842375E-4 68-69 0.0 2.302546293844511E-4 0.0 1.9490593868135018 5.526111105226826E-4 70-71 0.0 2.302546293844511E-4 0.0 2.206438011539441 6.216874993380179E-4 72-73 0.0 2.302546293844511E-4 0.0 2.4651981640416873 6.677384252149082E-4 74-75 0.0 2.302546293844511E-4 0.0 2.734803309587941 7.137893510917984E-4 76-77 0.0 2.302546293844511E-4 0.0 3.0348020662129422 7.368148140302435E-4 78-79 0.0 2.302546293844511E-4 0.0 3.3843055681556007 7.368148140302435E-4 80-81 4.605092587689022E-5 2.302546293844511E-4 0.0 3.7680939844136034 8.058912028455789E-4 82-83 4.605092587689022E-5 2.302546293844511E-4 0.0 4.146448391418133 9.210185175378044E-4 84-85 4.605092587689022E-5 2.302546293844511E-4 0.0 4.537996388686393 9.440439804762495E-4 86-87 4.605092587689022E-5 2.302546293844511E-4 0.0 4.960905066476814 0.0010131203692915849 88-89 4.605092587689022E-5 2.302546293844511E-4 0.0 5.426756232647436 0.001059171295168475 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8805 0.0 28.043533 1 TACATGG 8770 0.0 27.00388 2 ACATGGG 8680 0.0 25.537704 3 TACCTGG 1975 0.0 22.303429 2 CATGGGG 4615 0.0 21.142254 4 GAGTACA 6830 0.0 20.57926 1 GAGTACT 6350 0.0 20.57481 12-13 ATTCGCG 185 0.0 20.565063 80-81 GTACCTG 2210 0.0 19.982481 1 CATGGGA 4465 0.0 19.518753 4 AGTACAT 6460 0.0 19.209875 2 GTACTTT 6830 0.0 19.19882 14-15 AGTACTT 6810 0.0 18.976496 12-13 CGCGATC 225 0.0 18.940681 22-23 AGAGTAC 10700 0.0 18.82332 10-11 CCGCGAT 240 0.0 18.743382 22-23 CGCGGAA 210 1.8189894E-12 18.116213 82-83 TCGCGCT 250 0.0 18.076574 60-61 GTATCAA 14915 0.0 18.051718 1 GTACACG 320 8.314601E-9 17.80697 1 >>END_MODULE