FastQCFastQC Report
Fri 27 May 2016
SRR939118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939118_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2171509
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86610.39884706901974615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58820.2708715460078683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50200.23117564790198888No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38420.1769276572190122No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31600.1455209257709731No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28020.1290346943070464No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27250.12548877301452585No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23240.10702235173789287No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG953.5137855E-529.9730035
GTAAGCG3600.026.3657594-95
GTACATG89950.025.8592171
TACATGG89450.025.4131072
ACATGGG90700.023.964083
CATGGGG45300.022.0000514
TACCTGG18300.021.005672
GTACCTG21000.020.4277211
GAGTACT67350.020.22445912-13
GTACTTT71450.018.86464714-15
AGTACTT71350.018.7580512-13
AGAGTAC119450.017.89951310-11
GAGTACA77400.017.6125321
AGTACAT71150.017.4087752
CATGGGA48750.017.2306334
GTCGTAG3351.39807526E-817.0739151
GTATCAA177050.016.906761
TACCGGC2304.3068263E-516.506872
ATGGGGA25900.016.3077055
TACTTTT82200.016.13773514-15
Produced by FastQC (version 0.11.5)