##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939118_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2171509 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49477483169538 34.0 31.0 34.0 31.0 34.0 2 32.71058282512299 34.0 31.0 34.0 31.0 34.0 3 32.82145549477345 34.0 31.0 34.0 31.0 34.0 4 36.26419738532053 37.0 37.0 37.0 35.0 37.0 5 36.22455490628867 37.0 37.0 37.0 35.0 37.0 6 36.26818769804776 37.0 37.0 37.0 35.0 37.0 7 36.22228597717071 37.0 37.0 37.0 35.0 37.0 8 36.19945346761169 37.0 37.0 37.0 35.0 37.0 9 37.948148960008915 39.0 38.0 39.0 35.0 39.0 10-11 37.85868766834491 39.0 38.0 39.0 35.0 39.0 12-13 37.774685944198254 39.0 38.0 39.0 35.0 39.0 14-15 39.19193703549007 40.5 38.5 41.0 36.0 41.0 16-17 39.29539895989379 41.0 39.0 41.0 36.0 41.0 18-19 39.32025057229788 41.0 39.0 41.0 36.0 41.0 20-21 39.28418532918813 41.0 39.0 41.0 36.0 41.0 22-23 39.20938411952241 41.0 39.0 41.0 36.0 41.0 24-25 39.130103536296645 41.0 39.0 41.0 35.5 41.0 26-27 38.98494180774752 40.0 38.5 41.0 35.0 41.0 28-29 38.835244984018026 40.0 38.0 41.0 35.0 41.0 30-31 38.65903480022418 40.0 38.0 41.0 35.0 41.0 32-33 38.56789817587678 40.0 38.0 41.0 34.0 41.0 34-35 38.458896555344694 40.0 38.0 41.0 34.0 41.0 36-37 38.3074412770106 40.0 38.0 41.0 34.0 41.0 38-39 38.17816412457881 40.0 38.0 41.0 34.0 41.0 40-41 38.00181555775269 40.0 38.0 41.0 33.0 41.0 42-43 37.80681866849274 40.0 37.5 41.0 33.0 41.0 44-45 37.555519687001066 40.0 37.0 41.0 33.0 41.0 46-47 37.254833390052724 40.0 37.0 41.0 32.0 41.0 48-49 36.91193336062618 40.0 36.5 41.0 31.0 41.0 50-51 36.64696485255185 39.5 36.0 41.0 31.0 41.0 52-53 36.383153143735534 39.0 36.0 41.0 30.0 41.0 54-55 36.12758501116045 39.0 35.0 40.0 30.0 41.0 56-57 35.857403308022214 39.0 35.0 40.0 29.0 41.0 58-59 35.50124107245239 38.0 35.0 40.0 28.0 41.0 60-61 35.104008318639245 38.0 34.0 40.0 28.0 41.0 62-63 34.69485597342677 37.5 34.0 40.0 26.5 41.0 64-65 34.2226016562676 37.0 34.0 39.5 26.0 41.0 66-67 33.77856780699504 36.0 33.0 39.0 26.0 40.5 68-69 33.26279122029888 36.0 33.0 39.0 24.5 40.0 70-71 32.72101428085262 35.0 32.0 38.0 23.5 40.0 72-73 32.12245424725387 35.0 31.5 37.0 21.5 39.5 74-75 31.562462094331636 35.0 31.0 37.0 20.0 39.0 76-77 29.560458648801365 32.5 28.5 35.0 17.5 37.0 78-79 30.553888563206506 34.0 30.0 36.0 18.0 37.5 80-81 30.54490633011422 34.0 30.5 35.5 17.5 37.0 82-83 30.141325456168957 34.0 30.0 35.0 11.5 37.0 84-85 29.642704911653603 34.0 29.5 35.0 7.0 36.0 86-87 28.926274079453506 34.0 29.0 35.0 2.0 36.0 88-89 28.214782209053702 33.0 28.0 35.0 2.0 35.0 90-91 27.618850762304 33.0 26.5 35.0 2.0 35.0 92-93 27.1298265399775 33.0 26.0 34.5 2.0 35.0 94-95 26.5404350154662 32.0 25.0 34.0 2.0 35.0 96-97 25.669384976069637 32.0 23.5 34.0 2.0 35.0 98-99 24.37671637557109 32.0 10.0 34.0 2.0 35.0 100-101 18.45469694115935 24.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06457795886848317 1101 2 0.05583758927921423 1101 3 0.08992377813900987 1101 4 0.020845653979534973 1101 5 0.12723714811114917 1101 6 0.11695880347453169 1101 7 0.09186016648813933 1101 8 0.07699739193591881 1101 9 0.0887070884718213 1101 10-11 0.1636854563137149 1101 12-13 0.19770837180404754 1101 14-15 0.17467800685687251 1101 16-17 0.24225532508412329 1101 18-19 0.22137379840039273 1101 20-21 0.23764367171797574 1101 22-23 0.20747226148404252 1101 24-25 0.19692762524194762 1101 26-27 0.20507514315733744 1101 28-29 0.12506582773539776 1101 30-31 0.12796637547129563 1101 32-33 0.16504449020086298 1101 34-35 0.14175189853965264 1101 36-37 0.19574729014117764 1101 38-39 0.13864558661554582 1101 40-41 0.20798783045695046 1101 42-43 0.20912819332036747 1101 44-45 0.09212162970222693 1101 46-47 0.05890841404660563 1101 48-49 0.06981203727047358 1101 50-51 -0.007094895933938972 1101 52-53 -0.017099438072612827 1101 54-55 0.056085886704728694 1101 56-57 -0.024403980006852066 1101 58-59 -0.14898331861565595 1101 60-61 -0.1647238203762882 1101 62-63 -0.23827715586127596 1101 64-65 -0.20374225700067683 1101 66-67 -0.26692140179238066 1101 68-69 -0.13719448006606427 1101 70-71 -0.428193186994001 1101 72-73 -0.29411649014407004 1101 74-75 -0.535484102973637 1101 76-77 -0.2799983024855166 1101 78-79 -0.05782224388282842 1101 80-81 -0.018129040987719947 1101 82-83 -0.12076834282536808 1101 84-85 -0.18114673395888303 1101 86-87 0.04160133970904312 1101 88-89 0.07114288163439397 1101 90-91 0.2232688251567403 1101 92-93 0.23697162951825135 1101 94-95 -0.06777281893879561 1101 96-97 -0.2002512117948818 1101 98-99 -0.47830956746886955 1101 100-101 -0.9803715601964349 1102 1 -0.024481853372364526 1102 2 -0.006469926285852523 1102 3 0.042806215955600635 1102 4 0.06300900432817969 1102 5 0.10911698695112193 1102 6 0.04953467497585251 1102 7 0.09528568364200396 1102 8 0.0987882092578829 1102 9 0.051703117611218374 1102 10-11 0.08827711957490436 1102 12-13 0.06534546656298801 1102 14-15 0.14450061230717637 1102 16-17 0.10228849851340271 1102 18-19 0.08999899623064778 1102 20-21 0.104909703725248 1102 22-23 0.12098389314829205 1102 24-25 0.13773116687168852 1102 26-27 0.17702377745074926 1102 28-29 0.11968660579599089 1102 30-31 0.05833976941946872 1102 32-33 -0.05595908709563702 1102 34-35 -0.06046444320369204 1102 36-37 0.15204839687252303 1102 38-39 0.026510143540072306 1102 40-41 0.10623585522912293 1102 42-43 0.16445992481796878 1102 44-45 0.27767355025594753 1102 46-47 0.2672637129904061 1102 48-49 0.033890926748846084 1102 50-51 -0.0707438278043 1102 52-53 0.02895972176863637 1102 54-55 -0.1645599050383595 1102 56-57 0.007172551720614706 1102 58-59 -0.18170700027372533 1102 60-61 -0.18486938319253454 1102 62-63 -0.35312072810404516 1102 64-65 -0.12894523042223938 1102 66-67 -0.1844803427695041 1102 68-69 -0.17159402366586818 1102 70-71 -0.09818367213444645 1102 72-73 -0.08534191125487212 1102 74-75 -0.22129374093379184 1102 76-77 -0.3579748686498476 1102 78-79 -0.28834319641977757 1102 80-81 -0.09007552297741128 1102 82-83 -0.16665503884908617 1102 84-85 -0.1884960126017141 1102 86-87 -0.2739618846320049 1102 88-89 -0.10168976249588013 1102 90-91 -0.0769347771847464 1102 92-93 -0.058492013040563506 1102 94-95 -0.11953907763675886 1102 96-97 -0.14608792964910933 1102 98-99 -0.3131208160230514 1102 100-101 -1.1036594305394232 1103 1 0.1552825206243469 1103 2 0.13369779051470942 1103 3 0.16816843191491415 1103 4 0.15123113117434173 1103 5 0.17083798805157357 1103 6 0.11144334569291914 1103 7 0.16496414847189556 1103 8 0.13496438370250274 1103 9 0.22155173622684998 1103 10-11 0.19649911586829205 1103 12-13 0.21009191403154404 1103 14-15 0.15826889853543236 1103 16-17 0.16932326961358513 1103 18-19 0.23308579152443087 1103 20-21 0.21655843842405176 1103 22-23 0.2473708124128251 1103 24-25 0.24694318838896123 1103 26-27 0.17418139871387694 1103 28-29 0.23597472380699713 1103 30-31 0.28011181121593154 1103 32-33 0.3662545766667762 1103 34-35 0.4001568955388777 1103 36-37 0.2769742129769668 1103 38-39 0.2919684082651983 1103 40-41 0.30734533624375615 1103 42-43 0.4038215054204315 1103 44-45 0.5988237256590452 1103 46-47 0.5173827064067424 1103 48-49 0.3682923449871751 1103 50-51 0.4078713553748443 1103 52-53 0.42691327979418503 1103 54-55 0.43387416880120355 1103 56-57 0.3463100439214486 1103 58-59 0.19166469381718088 1103 60-61 0.3070649030672783 1103 62-63 0.22631274900052745 1103 64-65 0.21145916768321626 1103 66-67 0.22999317323980506 1103 68-69 0.21781512710463602 1103 70-71 0.20890488127007956 1103 72-73 0.16391189803037065 1103 74-75 0.06173502365072281 1103 76-77 0.16227277908829763 1103 78-79 0.21534247165957865 1103 80-81 0.3422013120098626 1103 82-83 0.36698800374865215 1103 84-85 0.02024577413270734 1103 86-87 0.1438143446447846 1103 88-89 0.1378687352115584 1103 90-91 0.17844875695507767 1103 92-93 -0.045656363211399764 1103 94-95 -0.07611477621650664 1103 96-97 -0.45575734825951386 1103 98-99 -0.45937555604079705 1103 100-101 -0.67678040890193 1104 1 0.06215034159531996 1104 2 0.09526739140792273 1104 3 0.044612806920063974 1104 4 0.06849385537181973 1104 5 0.08065201916480902 1104 6 0.08191455066044995 1104 7 0.12816601568040653 1104 8 0.09257674184240017 1104 9 0.0990079409778275 1104 10-11 0.14593340260139342 1104 12-13 0.13853458635680482 1104 14-15 0.07094092354208215 1104 16-17 0.21122824636514537 1104 18-19 0.2199134050171594 1104 20-21 0.29950708779873736 1104 22-23 0.31547760934194 1104 24-25 0.3169402575900975 1104 26-27 0.2834968751653193 1104 28-29 0.2665987820254685 1104 30-31 0.2463686974639998 1104 32-33 0.19644786325081043 1104 34-35 0.29166791499719835 1104 36-37 0.27965710293064916 1104 38-39 0.33834810109728153 1104 40-41 0.3638140982589064 1104 42-43 0.32103029016455054 1104 44-45 0.3280923093285679 1104 46-47 0.44308389937207693 1104 48-49 0.31739582078709105 1104 50-51 0.33654320394899884 1104 52-53 0.15979248544406488 1104 54-55 0.1923154939686853 1104 56-57 0.3456128167016317 1104 58-59 0.18570836989455586 1104 60-61 0.1615383835888906 1104 62-63 0.1409006874479104 1104 64-65 0.10908886084744296 1104 66-67 0.16927076718944534 1104 68-69 0.08364100257784202 1104 70-71 0.029650925023737784 1104 72-73 -0.1734020606764517 1104 74-75 -0.3433869392989841 1104 76-77 -0.4023869413459096 1104 78-79 -0.03154212634136755 1104 80-81 -0.03076835249470733 1104 82-83 -0.06809054989811969 1104 84-85 -0.19909635584862073 1104 86-87 -0.1740228214454227 1104 88-89 -0.018201353129576603 1104 90-91 0.05652114689003085 1104 92-93 0.010785694788658873 1104 94-95 -0.048539891382553435 1104 96-97 -0.1359003754957513 1104 98-99 -0.5102581303822511 1104 100-101 -1.2131967141344049 1105 1 0.06473786874038723 1105 2 0.07097411668500797 1105 3 0.07407314735215209 1105 4 0.07446287153169351 1105 5 0.01678228354303002 1105 6 0.09159232995863675 1105 7 0.03224351625442523 1105 8 -0.025415372139697467 1105 9 0.08674263453457343 1105 10-11 0.06426678360851668 1105 12-13 0.04780157220593395 1105 14-15 -0.00404685521751702 1105 16-17 0.05202806824556916 1105 18-19 0.09324942869840669 1105 20-21 0.1035269675113284 1105 22-23 0.09253242054213473 1105 24-25 0.052941106956645 1105 26-27 0.12238446268859349 1105 28-29 0.13308730333655205 1105 30-31 0.1646364947983372 1105 32-33 0.067576075079117 1105 34-35 0.08385541781575512 1105 36-37 0.13869227819882468 1105 38-39 0.13239088742155758 1105 40-41 0.15332168985893446 1105 42-43 0.11444494209820988 1105 44-45 0.11519057067834382 1105 46-47 0.1355458228316806 1105 48-49 0.03827389983744922 1105 50-51 -0.015655163339673095 1105 52-53 0.14643519152174633 1105 54-55 0.0038988848326866332 1105 56-57 -0.09995389452070214 1105 58-59 -0.08559311683190174 1105 60-61 -0.2849716244280387 1105 62-63 -0.265467860838811 1105 64-65 -0.18063983405808415 1105 66-67 -0.14086849439252092 1105 68-69 -0.3300714997317655 1105 70-71 -0.32271146587093114 1105 72-73 -0.4853401703050366 1105 74-75 -0.25287206494776626 1105 76-77 -0.5043497099200707 1105 78-79 -0.5949621821635098 1105 80-81 -0.32043932346034154 1105 82-83 -0.27217044704642746 1105 84-85 -0.5051939108532189 1105 86-87 -0.42812721261376296 1105 88-89 -0.33700590579609724 1105 90-91 -0.3710700975221535 1105 92-93 -0.3818667176725974 1105 94-95 -0.23595032586085907 1105 96-97 -0.5619674759681317 1105 98-99 -0.735835041218607 1105 100-101 -1.6170631615531796 1106 1 0.01128971889420427 1106 2 0.03906292229648756 1106 3 0.036354682580046926 1106 4 0.0529369901975727 1106 5 0.09771419318703778 1106 6 0.05191374532021342 1106 7 0.04085032506853992 1106 8 0.15949148340721564 1106 9 0.12309012281261289 1106 10-11 0.06822246053646097 1106 12-13 0.17898010258114994 1106 14-15 0.1169534482134722 1106 16-17 0.030228656060039327 1106 18-19 0.010224509705622609 1106 20-21 0.11868412039846277 1106 22-23 0.13378796037243745 1106 24-25 0.14718632791862518 1106 26-27 0.06116338553075451 1106 28-29 0.2055430788242063 1106 30-31 0.16530869409712778 1106 32-33 0.1933050292610332 1106 34-35 0.09673794572808703 1106 36-37 0.00882381663821974 1106 38-39 0.22434265739871506 1106 40-41 0.02977766025116324 1106 42-43 0.1460471684400062 1106 44-45 0.2313503747106509 1106 46-47 0.277025502922406 1106 48-49 0.10357246928217023 1106 50-51 0.0979354440317124 1106 52-53 0.2234601503813778 1106 54-55 0.14468200255283392 1106 56-57 0.13105010766754788 1106 58-59 0.2070660681568981 1106 60-61 0.18816365927992962 1106 62-63 0.19472287986501158 1106 64-65 0.14367584047785442 1106 66-67 0.12914964502184034 1106 68-69 -0.07534118786229982 1106 70-71 -0.11879034020841317 1106 72-73 -0.07680689367121829 1106 74-75 -0.31960694821543 1106 76-77 -0.22039146379545826 1106 78-79 -0.23207422793064936 1106 80-81 0.08498876893328955 1106 82-83 -0.06098248151796071 1106 84-85 -0.23721341016994657 1106 86-87 -0.21960921827042412 1106 88-89 -0.019546221142839215 1106 90-91 0.22404997919937486 1106 92-93 0.4107692116567776 1106 94-95 0.31368721807572797 1106 96-97 -0.141943688750775 1106 98-99 0.008652813790110514 1106 100-101 -0.42079536266811957 1107 1 -0.03340156109917558 1107 2 -0.05150597543455149 1107 3 -0.028453435420260575 1107 4 -0.05694112149398478 1107 5 -0.12440904972397959 1107 6 -0.05992227141294393 1107 7 -0.06380521396690142 1107 8 0.05610617886555502 1107 9 -0.09451330055183149 1107 10-11 0.028889614087077575 1107 12-13 -0.016546928079577583 1107 14-15 -0.08552193009371223 1107 16-17 -0.04024520267903853 1107 18-19 0.0017753293368016898 1107 20-21 -0.034519238959354936 1107 22-23 -0.017178113054661992 1107 24-25 -0.07087723141039959 1107 26-27 -0.14523416581535997 1107 28-29 -0.06669880964449248 1107 30-31 -0.07160300056771973 1107 32-33 -0.09341953558938343 1107 34-35 -0.15534221054610242 1107 36-37 0.0364323280105836 1107 38-39 -0.12519700632142872 1107 40-41 -0.04613122316558815 1107 42-43 -0.1097425910190637 1107 44-45 -0.17283458839951749 1107 46-47 -0.21771669249999803 1107 48-49 -0.3700818303908875 1107 50-51 -0.20938388749973313 1107 52-53 -0.1633522939574874 1107 54-55 -0.34000389485133553 1107 56-57 -0.37663657850747256 1107 58-59 -0.42795443823539614 1107 60-61 -0.49156273833740016 1107 62-63 -0.3616542039735293 1107 64-65 -0.42324607497920397 1107 66-67 -0.3540325916796405 1107 68-69 -0.5368027682178251 1107 70-71 -0.6826612183976408 1107 72-73 -0.7748269068166138 1107 74-75 -0.7422251583819737 1107 76-77 -0.7159249045449094 1107 78-79 -0.5075190397768701 1107 80-81 -0.5028384018774297 1107 82-83 -0.6188758341381195 1107 84-85 -0.6643211569271372 1107 86-87 -0.7105395318863721 1107 88-89 -0.8322788961694911 1107 90-91 -0.5302734724637226 1107 92-93 -0.4725935680216402 1107 94-95 -0.40939282484358586 1107 96-97 -0.6043313148286167 1107 98-99 -0.3883430698488688 1107 100-101 -1.026041478260403 1108 1 -0.09349226352664175 1108 2 -0.043034005201768366 1108 3 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98-99 -0.3467510426937679 2316 100-101 0.9901314409957997 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 15.0 5 35.0 6 84.0 7 185.0 8 496.0 9 904.0 10 1633.0 11 2652.0 12 4024.0 13 5659.0 14 7043.0 15 9692.0 16 12386.0 17 17314.0 18 17659.0 19 13004.0 20 12411.0 21 13499.0 22 14130.0 23 15707.0 24 17853.0 25 20729.0 26 24139.0 27 28698.0 28 35188.0 29 42141.0 30 51647.0 31 63424.0 32 79962.0 33 104187.0 34 144953.0 35 215152.0 36 345675.0 37 518304.0 38 317497.0 39 13422.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.653774112837205 19.02022449045661 13.996290866374068 23.32971053033211 2 15.778290580421265 20.66498457984747 39.40738905526065 24.149335784470615 3 20.277880496926333 24.049727631798902 29.265731802170748 26.40666006910402 4 13.166558370239311 16.312205015037932 36.43586096120256 34.0853756535202 5 13.953154235142476 37.32224918248094 33.8709625426374 14.853634039739186 6 32.63601486339683 35.51640817514456 17.104925653082717 14.742651308375882 7 29.10059318197622 32.20875437311105 20.372607251455094 18.31804519345764 8 26.231159990587187 35.0135781154948 19.454950451506303 19.300311442411704 9 26.071915888904908 17.337436777835137 20.410921621784667 36.17972571147529 10-11 25.42054856783923 26.36314194415036 26.8684127028716 21.347896785138815 12-13 26.30511777754548 23.60832950726891 27.5533971998274 22.533155515358214 14-15 22.678593344064826 25.276831742989437 26.095002487922464 25.94957242502327 16-17 22.31514513205095 28.462778502970476 26.66921027781622 22.552866087162354 18-19 22.179512537022074 27.35846993000488 28.639238575395858 21.822778957577185 20-21 22.991592482462657 26.2529651039899 28.54369933534699 22.211743078200456 22-23 22.53555016350381 26.22107483782015 28.625831161648417 22.617543837027615 24-25 22.69111019111595 26.384601675608987 28.686618383805918 22.23766974946915 26-27 22.446287872961545 26.40488763892298 28.906157535309568 22.242666952805905 28-29 22.006435159345486 27.107498567815547 28.759000657607526 22.12706561523144 30-31 22.340179110471105 26.460194270435906 28.565734703379082 22.63389191571391 32-33 22.176288971468452 26.61838532982947 28.669217735044555 22.53610796365752 34-35 22.872719166782577 26.534902079266924 28.464485369230385 22.12789338472011 36-37 22.757344583777552 26.234438455477626 28.717767654650423 22.2904493060944 38-39 22.29095628967767 26.77333817268661 28.71720136760861 22.218504170027114 40-41 22.479260283044805 26.68389737656915 28.519350237666817 22.317492102719225 42-43 22.297128480018827 26.716978924900747 28.08152603052586 22.904366564554564 44-45 22.550051279736834 26.58058793703484 28.138874830007786 22.73048595322054 46-47 22.836108316567994 27.035322231865283 27.77090943170391 22.357660019862813 48-49 22.789838311656762 26.936758970641705 27.796307549332113 22.477095168369424 50-51 22.36463303746197 27.18446803136364 27.9745686785904 22.47633025258399 52-53 22.77842274657853 27.3472502301395 27.332444857470083 22.541882165811884 54-55 22.292171020244446 26.918424008373897 27.73808443805667 23.051320533324983 56-57 22.58999276079112 27.103100702369044 27.601786408339272 22.705120128500564 58-59 22.93073252770805 26.799797004901265 27.571344165571794 22.698126301818895 60-61 22.560277528073332 26.933863730142356 28.13374988947508 22.372108852309232 62-63 22.3413833073065 26.99747683258906 28.046601460664792 22.614538399439642 64-65 22.108076468930353 27.24891823137046 27.723372097092998 22.919633202606192 66-67 22.280336895738515 27.12644826806504 27.43557023274063 23.15764460345581 68-69 22.19537327747095 26.982728944244688 27.686396956812576 23.135500821471787 70-71 22.492789569639886 26.924596605030516 27.57347777373041 23.00913605159919 72-73 22.479391059396946 26.686626672972576 27.81786766713838 23.0161146004921 74-75 22.154018242613777 27.49281720683635 27.263944105228205 23.08922044532166 76-77 22.293242457752395 26.85811784109342 27.456344181582757 23.392295519571423 78-79 22.393747259509272 26.946070827176825 27.546215267158697 23.113966646155205 80-81 22.42505655066719 27.096818610511423 27.405130269159073 23.072994569662317 82-83 22.453625566368824 26.936591098632334 27.51508282949783 23.094700505501013 84-85 22.266612756382774 26.975043621739538 27.45823296150281 23.30011066037488 86-87 22.25180858555183 26.922448358644502 27.432574939464104 23.393168116339563 88-89 22.14423665849175 26.863709437442672 27.192679242648065 23.799374661417517 90-91 22.20112592707696 26.711493788809822 27.37342569764608 23.71395458646714 92-93 22.42182740205083 26.762311369651243 27.341701093571334 23.47416013472659 94-95 22.164730186411887 26.529829378977794 27.493007740932168 23.81243269367815 96-97 21.954070177482613 26.396327714121316 27.778040012277177 23.871562096118897 98-99 22.010139506867585 26.590578151752748 27.161218988112395 24.238063353267272 100-101 22.300027999027403 25.243287601588577 28.065663245381998 24.39102115400202 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 415.0 1 294.0 2 256.0 3 367.0 4 319.5 5 278.0 6 451.5 7 913.5 8 1467.5 9 1740.0 10 1679.5 11 1627.0 12 1732.0 13 1846.5 14 2021.5 15 2269.5 16 2411.5 17 2567.0 18 2802.0 19 3053.0 20 3427.0 21 3930.5 22 4635.5 23 5727.5 24 7061.0 25 8701.0 26 11220.0 27 14249.0 28 16856.5 29 19790.0 30 23329.0 31 27175.5 32 31652.5 33 35142.0 34 38365.5 35 42054.0 36 46487.5 37 51329.5 38 54860.5 39 57198.0 40 59568.0 41 63883.5 42 68118.5 43 70908.0 44 74266.0 45 78794.5 46 81029.5 47 87082.5 48 101300.5 49 101103.0 50 88807.0 51 82376.0 52 78570.0 53 77000.0 54 75153.0 55 69596.0 56 62167.0 57 55519.5 58 49855.0 59 43568.5 60 36585.5 61 30103.0 62 24353.5 63 19068.0 64 14563.0 65 11042.5 66 8381.0 67 6712.0 68 5145.5 69 3658.5 70 2823.0 71 2182.5 72 1692.0 73 1422.0 74 1178.5 75 956.5 76 629.0 77 329.5 78 210.0 79 129.0 80 73.0 81 40.5 82 28.5 83 17.5 84 8.0 85 4.5 86 3.0 87 4.0 88 3.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42730654121166434 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.51942128722469E-4 16-17 0.0025558263861674073 18-19 2.302546293844511E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0033156666631360956 28-29 4.605092587689022E-5 30-31 0.0 32-33 2.302546293844511E-5 34-35 0.012111393505622127 36-37 0.02636415506451965 38-39 0.019341388868293893 40-41 0.008841777768362922 42-43 0.00932531249007027 44-45 0.0039603796254125585 46-47 0.00527283101290393 48-49 0.003614997681335882 50-51 6.21687499338018E-4 52-53 0.0 54-55 0.0 56-57 4.605092587689022E-5 58-59 0.0012894259245529263 60-61 0.002440699071475182 62-63 0.001473629628060487 64-65 0.002993310181997864 66-67 0.0016578333315680478 68-69 0.004674168976504357 70-71 9.210185175378044E-5 72-73 0.0 74-75 0.0 76-77 0.0965227406379619 78-79 0.01680858794506493 80-81 2.302546293844511E-4 82-83 0.0 84-85 0.0 86-87 0.10782824294073845 88-89 0.41448135835495037 90-91 0.4237606199191438 92-93 0.0 94-95 2.302546293844511E-5 96-97 2.302546293844511E-5 98-99 1.3815277763067066E-4 100-101 2.302546293844511E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2171509.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.17795301951237 #Duplication Level Percentage of deduplicated Percentage of total 1 78.60846055501017 45.73279325105574 2 12.440715895945315 14.475507698468146 3 3.8172926134424987 6.662468109797678 4 1.6850928320290066 3.921410064612024 5 0.8998744252204263 2.617642601696733 6 0.5622096628550846 1.96249244116194 7 0.35541617984309287 1.4474170069300212 8 0.2589390102953394 1.205163326070503 9 0.19065236431080781 0.9982587864539819 >10 1.0710615101528957 11.238301827589964 >50 0.07179459560700767 2.8641308835933588 >100 0.03508710306689228 3.814435924587235 >500 0.0020577804129701016 0.8518817626814428 >1k 0.0011080356069838734 1.3073145779174686 >5k 2.3743620149654427E-4 0.9007817373837502 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8661 0.39884706901974615 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5882 0.2708715460078683 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5020 0.23117564790198888 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3842 0.1769276572190122 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3160 0.1455209257709731 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2802 0.1290346943070464 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2725 0.12548877301452585 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2324 0.10702235173789287 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.605092587689022E-5 2 0.0 0.0 0.0 0.0 4.605092587689022E-5 3 0.0 0.0 0.0 0.0 4.605092587689022E-5 4 0.0 0.0 0.0 0.0 4.605092587689022E-5 5 0.0 0.0 0.0 0.0 4.605092587689022E-5 6 0.0 0.0 0.0 0.0 4.605092587689022E-5 7 0.0 0.0 0.0 0.0 4.605092587689022E-5 8 0.0 0.0 0.0 0.0 4.605092587689022E-5 9 0.0 0.0 0.0 0.0 4.605092587689022E-5 10-11 0.0 0.0 0.0 0.0 4.605092587689022E-5 12-13 0.0 0.0 0.0 0.0 9.210185175378044E-5 14-15 0.0 0.0 0.0 0.0 9.210185175378044E-5 16-17 0.0 0.0 0.0 4.605092587689022E-5 9.210185175378044E-5 18-19 0.0 0.0 0.0 1.8420370350756088E-4 9.210185175378044E-5 20-21 0.0 0.0 0.0 2.302546293844511E-4 1.6117824056911578E-4 22-23 0.0 0.0 0.0 4.374837958304571E-4 1.8420370350756088E-4 24-25 0.0 0.0 0.0 6.216874993380179E-4 1.8420370350756088E-4 26-27 0.0 0.0 0.0 9.900949063531396E-4 2.763055552613413E-4 28-29 0.0 0.0 0.0 0.002555826386167407 2.763055552613413E-4 30-31 0.0 0.0 0.0 0.007022766196225758 2.993310181997864E-4 32-33 0.0 0.0 0.0 0.017522377296156726 3.223564811382315E-4 34-35 0.0 0.0 0.0 0.03534408561051324 3.223564811382315E-4 36-37 0.0 0.0 0.0 0.06767183557609018 3.223564811382315E-4 38-39 0.0 0.0 0.0 0.11353855774947283 3.223564811382315E-4 40-41 0.0 0.0 0.0 0.16209925908665357 3.223564811382315E-4 42-43 0.0 2.302546293844511E-5 0.0 0.21777482847181384 3.223564811382315E-4 44-45 0.0 4.605092587689022E-5 0.0 0.28270663395822904 3.223564811382315E-4 46-47 0.0 4.605092587689022E-5 0.0 0.36290432137283335 3.223564811382315E-4 48-49 0.0 4.605092587689022E-5 0.0 0.44618742082118934 3.223564811382315E-4 50-51 0.0 4.605092587689022E-5 0.0 0.539693825814215 3.6840740701512176E-4 52-53 0.0 4.605092587689022E-5 0.0 0.6322331613638259 3.6840740701512176E-4 54-55 0.0 4.605092587689022E-5 0.0 0.7434231218935772 3.6840740701512176E-4 56-57 0.0 4.605092587689022E-5 0.0 0.8753129736049908 3.9143286995356686E-4 58-59 0.0 4.605092587689022E-5 0.0 1.0199359063213644 4.1445833289201195E-4 60-61 0.0 4.605092587689022E-5 0.0 1.1862948760516305 4.1445833289201195E-4 62-63 0.0 4.605092587689022E-5 0.0 1.3518479545790507 4.1445833289201195E-4 64-65 0.0 4.605092587689022E-5 0.0 1.5220291511570987 4.1445833289201195E-4 66-67 0.0 4.605092587689022E-5 0.0 1.7253670143665074 4.1445833289201195E-4 68-69 0.0 4.605092587689022E-5 0.0 1.9629667664283224 4.1445833289201195E-4 70-71 0.0 4.605092587689022E-5 0.0 2.221588766152938 4.1445833289201195E-4 72-73 0.0 4.605092587689022E-5 0.0 2.4811087589321525 5.295856475842375E-4 74-75 0.0 9.210185175378044E-5 0.0 2.7529473743834356 5.986620363995728E-4 76-77 0.0 9.210185175378044E-5 0.0 3.0531303347119447 5.986620363995728E-4 78-79 0.0 9.210185175378044E-5 0.0 3.402472658414034 6.216874993380179E-4 80-81 0.0 9.210185175378044E-5 0.0 3.7866064566161137 6.44712962276463E-4 82-83 0.0 9.210185175378044E-5 0.0 4.164822710843013 6.44712962276463E-4 84-85 4.605092587689022E-5 9.210185175378044E-5 0.0 4.555679944223119 6.44712962276463E-4 86-87 4.605092587689022E-5 9.210185175378044E-5 0.0 4.978197189143586 6.44712962276463E-4 88-89 4.605092587689022E-5 9.210185175378044E-5 0.0 5.442068165501501 6.44712962276463E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGATCG 95 3.5137855E-5 29.973003 5 GTAAGCG 360 0.0 26.36575 94-95 GTACATG 8995 0.0 25.859217 1 TACATGG 8945 0.0 25.413107 2 ACATGGG 9070 0.0 23.96408 3 CATGGGG 4530 0.0 22.000051 4 TACCTGG 1830 0.0 21.00567 2 GTACCTG 2100 0.0 20.427721 1 GAGTACT 6735 0.0 20.224459 12-13 GTACTTT 7145 0.0 18.864647 14-15 AGTACTT 7135 0.0 18.75805 12-13 AGAGTAC 11945 0.0 17.899513 10-11 GAGTACA 7740 0.0 17.612532 1 AGTACAT 7115 0.0 17.408775 2 CATGGGA 4875 0.0 17.230633 4 GTCGTAG 335 1.39807526E-8 17.073915 1 GTATCAA 17705 0.0 16.90676 1 TACCGGC 230 4.3068263E-5 16.50687 2 ATGGGGA 2590 0.0 16.307705 5 TACTTTT 8220 0.0 16.137735 14-15 >>END_MODULE