FastQCFastQC Report
Fri 27 May 2016
SRR939117_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939117_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1780128
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55220.31020241241079294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44870.2520605259846483No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29550.16599929892681875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28640.16088730698017223No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG81150.023.689441
TACATGG82200.022.8188932
GTATCAA117950.021.9324341
ACATGGG83550.021.7698783
GAGTACT49450.021.02043712-13
GTACTTT52850.019.53454614-15
ATCAACG134000.019.0172943
TCAACGC135200.018.918574
GGTATCA90350.018.7554861
AGTACTT52650.018.75345212-13
CAACGCA136000.018.7376275
TATCAAC136800.018.6626742
CATGGGG40400.018.5245174
AACGCAG137850.018.4814836
CTAGGCG1300.007688490718.217884
GTACCTG15500.018.0679361
TACCTGG14250.017.9494082
CATGGGA46250.017.6152134
ACGCAGA149600.016.9670647
AGAGTAC112200.016.83997210-11
Produced by FastQC (version 0.11.5)