##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939117_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1780128 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2093422495461 33.0 31.0 34.0 31.0 34.0 2 32.29948576731561 34.0 31.0 34.0 31.0 34.0 3 32.36733594438153 34.0 31.0 34.0 30.0 34.0 4 35.78678555699366 37.0 35.0 37.0 35.0 37.0 5 35.83688588685757 37.0 35.0 37.0 35.0 37.0 6 35.92045178773661 37.0 37.0 37.0 35.0 37.0 7 35.850631527620486 37.0 37.0 37.0 35.0 37.0 8 35.84051540113969 37.0 37.0 37.0 35.0 37.0 9 37.54657811123695 39.0 38.0 39.0 35.0 39.0 10-11 37.46324871020511 39.0 38.0 39.0 35.0 39.0 12-13 37.37903678836578 39.0 38.0 39.0 35.0 39.0 14-15 38.73080559375506 40.5 38.0 41.0 35.0 41.0 16-17 38.676666228495925 40.0 38.0 41.0 35.0 41.0 18-19 38.60591092325945 40.0 38.0 41.0 34.0 41.0 20-21 38.50866538810692 40.0 38.0 41.0 34.0 41.0 22-23 38.437166597008755 40.0 38.0 41.0 34.0 41.0 24-25 38.480112385176795 40.0 38.0 41.0 34.0 41.0 26-27 38.41108364117636 40.0 38.0 41.0 34.0 41.0 28-29 38.29876531350555 40.0 38.0 41.0 34.0 41.0 30-31 38.14683241879236 40.0 38.0 41.0 34.0 41.0 32-33 37.9696791466681 40.0 38.0 41.0 33.5 41.0 34-35 37.67622637248557 40.0 38.0 41.0 33.0 41.0 36-37 37.441067720972875 40.0 37.5 41.0 32.0 41.0 38-39 37.229215539556705 40.0 37.0 41.0 31.5 41.0 40-41 37.09399183654209 40.0 37.0 41.0 31.0 41.0 42-43 36.91385226230923 40.0 37.0 41.0 31.0 41.0 44-45 36.67232412500674 40.0 36.5 41.0 30.0 41.0 46-47 36.33067144609826 40.0 36.0 41.0 30.0 41.0 48-49 36.32895331122256 40.0 36.0 41.0 30.0 41.0 50-51 35.33240924248144 38.5 35.0 40.0 27.5 40.5 52-53 35.49247357493394 38.5 35.0 40.0 28.0 40.5 54-55 35.98966843957288 39.0 36.0 41.0 29.0 41.0 56-57 35.88969023575832 39.0 35.0 41.0 28.0 41.0 58-59 35.708291763288926 39.0 35.0 41.0 28.0 41.0 60-61 35.428104889086626 39.0 35.0 41.0 28.0 41.0 62-63 35.07736747020439 38.0 35.0 40.5 27.0 41.0 64-65 34.713194500620176 38.0 34.5 40.0 26.0 41.0 66-67 34.33000632538784 37.0 34.0 40.0 26.0 41.0 68-69 33.940442204156106 36.5 34.0 39.0 26.0 41.0 70-71 33.539958081666036 36.0 34.0 39.0 25.0 41.0 72-73 33.092027371065456 35.5 33.5 39.0 24.0 40.0 74-75 32.64631897256827 35.0 33.0 37.5 22.5 39.5 76-77 32.21621254201945 35.0 33.0 37.0 21.5 39.0 78-79 31.799405155134913 35.0 32.0 36.5 20.0 39.0 80-81 31.383740101835375 35.0 32.0 36.0 19.0 38.0 82-83 30.982102691491846 35.0 31.5 35.5 17.5 37.0 84-85 30.621531148321917 35.0 31.0 35.0 13.0 37.0 86-87 30.253976399449925 34.5 31.0 35.0 7.0 36.0 88-89 29.88538464649733 34.0 31.0 35.0 2.0 36.0 90-91 29.545102936418054 34.0 30.5 35.0 2.0 36.0 92-93 29.17117111803196 34.0 30.0 35.0 2.0 35.0 94-95 28.7569385459922 34.0 29.5 35.0 2.0 35.0 96-97 28.237243333063688 34.0 29.0 35.0 2.0 35.0 98-99 27.59987680661166 34.0 29.0 35.0 2.0 35.0 100-101 25.114398234284277 31.5 22.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10600196383902016 1101 2 0.20933664415608177 1101 3 0.22622233150867999 1101 4 0.18381132988069027 1101 5 0.18239044950233563 1101 6 0.2897405666689892 1101 7 0.21175217571541083 1101 8 0.3760036643518916 1101 9 0.39189804027468256 1101 10-11 0.3582377636426415 1101 12-13 0.27694518570492477 1101 14-15 0.4074416269606971 1101 16-17 0.46659105169739945 1101 18-19 0.43461572087446854 1101 20-21 0.5581740181389918 1101 22-23 0.4639798485759812 1101 24-25 0.4340981974411733 1101 26-27 0.40787744479253973 1101 28-29 0.4775965289027724 1101 30-31 0.36801381352589857 1101 32-33 0.4279680817432947 1101 34-35 0.43201591671194706 1101 36-37 0.3802768277127342 1101 38-39 0.4704548209956556 1101 40-41 0.3293740450879383 1101 42-43 0.2972978218338085 1101 44-45 0.30858815047528054 1101 46-47 0.1942644256191599 1101 48-49 0.1282614580935899 1101 50-51 0.018804907047474728 1101 52-53 0.2012388755858936 1101 54-55 0.21225777559079972 1101 56-57 0.2848705282809334 1101 58-59 0.07111527012018115 1101 60-61 0.09137106315666443 1101 62-63 0.08857370249453567 1101 64-65 -0.02646968507555414 1101 66-67 0.00265001077914917 1101 68-69 -0.018814824773052408 1101 70-71 0.09492221400167722 1101 72-73 0.038034367963057036 1101 74-75 -0.001919163817213132 1101 76-77 -0.12893572246031226 1101 78-79 -0.05774251303453326 1101 80-81 -0.06470249154704533 1101 82-83 0.06710220048643123 1101 84-85 -5.477263881381589E-4 1101 86-87 -0.12019255529378725 1101 88-89 -0.13085534981727065 1101 90-91 -0.41419306478632834 1101 92-93 -0.5502434909415612 1101 94-95 -0.4902713862054213 1101 96-97 -0.5061494438402754 1101 98-99 -0.45872699390625016 1101 100-101 -0.6818359139967036 1102 1 0.11889531576155576 1102 2 0.21339250814692434 1102 3 0.18027262870883476 1102 4 0.22953088327452065 1102 5 0.20407309922823202 1102 6 0.19781872773369003 1102 7 0.2402051230402762 1102 8 0.20051183424037333 1102 9 0.3716502931631851 1102 10-11 0.24412831721428319 1102 12-13 0.2348045802042691 1102 14-15 0.3428915732037723 1102 16-17 0.35736957983793616 1102 18-19 0.3365965844083334 1102 20-21 0.4545108206076591 1102 22-23 0.43199967465947253 1102 24-25 0.2619497651989846 1102 26-27 0.25137584870225993 1102 28-29 0.2587072033485427 1102 30-31 0.3494826320202691 1102 32-33 0.3230238992057366 1102 34-35 0.3078112615455524 1102 36-37 0.38151232289953896 1102 38-39 0.24490736452047912 1102 40-41 0.37854625500413874 1102 42-43 0.3731006859891579 1102 44-45 0.3326080214254361 1102 46-47 0.34653671479981796 1102 48-49 0.39308968521200427 1102 50-51 0.4246798237533298 1102 52-53 0.3400218754380049 1102 54-55 0.2577502953196671 1102 56-57 0.3373092811204117 1102 58-59 0.25674032101061783 1102 60-61 0.1717499123431665 1102 62-63 0.09381707925991378 1102 64-65 0.24957832558609994 1102 66-67 0.3707380508201581 1102 68-69 0.3931970632330959 1102 70-71 0.08318542515919347 1102 72-73 0.025348524394857463 1102 74-75 -0.04337596801408239 1102 76-77 0.012844412571752173 1102 78-79 -0.06280455367044269 1102 80-81 -0.07787667559677658 1102 82-83 -0.2301457277534844 1102 84-85 -0.2268795187451822 1102 86-87 -0.3074016489371765 1102 88-89 -0.2859760494714685 1102 90-91 -0.36716448471677054 1102 92-93 -0.47684660202977724 1102 94-95 -0.43894453749085116 1102 96-97 -0.5721626495971712 1102 98-99 -0.6665726791449451 1102 100-101 -0.4393703990187099 1103 1 0.14791891823804093 1103 2 0.22929134273345397 1103 3 0.22449330969357106 1103 4 0.1749349838450911 1103 5 0.17894291664303807 1103 6 0.19985245382535055 1103 7 0.1090619439003575 1103 8 0.11713388754810694 1103 9 0.2861176418113587 1103 10-11 0.26027212335275607 1103 12-13 0.28408988223269205 1103 14-15 0.24464029629709927 1103 16-17 0.33033730066603084 1103 18-19 0.3486297317320961 1103 20-21 0.27542114212929647 1103 22-23 0.1767809164703138 1103 24-25 0.19062144463873665 1103 26-27 0.27283768780156237 1103 28-29 0.3450294307689532 1103 30-31 0.22671752014925772 1103 32-33 0.2063195724333795 1103 34-35 0.05315780686387939 1103 36-37 0.026442235714590367 1103 38-39 0.09410878539567591 1103 40-41 0.14110511650337543 1103 42-43 0.2129646514174226 1103 44-45 0.06704800721347226 1103 46-47 0.0593297763373144 1103 48-49 0.02189508415189323 1103 50-51 0.162342622321205 1103 52-53 0.1281522300458775 1103 54-55 0.07355238724408508 1103 56-57 0.03595970691674211 1103 58-59 -0.07110597109674899 1103 60-61 0.06823948407100033 1103 62-63 -0.176053349076561 1103 64-65 -0.2824910429620431 1103 66-67 -0.3705874643234637 1103 68-69 -0.46633717329969926 1103 70-71 -0.38180956689078727 1103 72-73 -0.5705708001830061 1103 74-75 -0.5301905098790982 1103 76-77 -0.5758668401231333 1103 78-79 -0.4584498879169452 1103 80-81 -0.6627811226242422 1103 82-83 -0.5551564590749543 1103 84-85 -0.5263595520564941 1103 86-87 -0.5054307030857359 1103 88-89 -0.4483542067709365 1103 90-91 -0.6200386300293097 1103 92-93 -0.5968219722402743 1103 94-95 -0.7452600608497626 1103 96-97 -0.7186614655345096 1103 98-99 -0.88126261228118 1103 100-101 -0.9581982412915977 1104 1 0.08421193743031097 1104 2 0.1732528639103137 1104 3 0.12038772863003544 1104 4 0.04229005186812884 1104 5 0.16649284577587764 1104 6 0.14580092664598965 1104 7 0.18936770975275863 1104 8 0.20243178101795678 1104 9 0.332521487179541 1104 10-11 0.2952531573532795 1104 12-13 0.3005922266627863 1104 14-15 0.28577113812349353 1104 16-17 0.3841519492686416 1104 18-19 0.27312772985278144 1104 20-21 0.23782246236399374 1104 22-23 0.288850606697558 1104 24-25 0.2806341253028535 1104 26-27 0.33778798651213293 1104 28-29 0.31210723693550335 1104 30-31 0.1392621853137328 1104 32-33 0.12930869723185623 1104 34-35 0.09670673505706873 1104 36-37 0.11401561700503038 1104 38-39 0.26960104500516735 1104 40-41 0.2257683875176042 1104 42-43 0.10272890420104375 1104 44-45 0.1935596303699043 1104 46-47 0.10298201973063925 1104 48-49 0.272020005192374 1104 50-51 0.20298006532107138 1104 52-53 0.26074373667219675 1104 54-55 0.276599360697972 1104 56-57 0.23051970987084758 1104 58-59 0.1249236218334957 1104 60-61 0.21973001207059895 1104 62-63 0.24108216432534135 1104 64-65 0.15233277908753706 1104 66-67 0.18024106072831358 1104 68-69 0.06972317063384992 1104 70-71 0.08436177606539985 1104 72-73 0.0746575696209888 1104 74-75 -0.09993043055816031 1104 76-77 -0.2394574243764822 1104 78-79 -0.21219143021253828 1104 80-81 -0.1984934161116918 1104 82-83 -0.024034031741287265 1104 84-85 -0.06327470160033499 1104 86-87 -0.18425458327467226 1104 88-89 -0.055063291594681374 1104 90-91 -0.0961136973609662 1104 92-93 -0.20003758909880176 1104 94-95 -0.4448424395977888 1104 96-97 -0.4217164920743386 1104 98-99 -0.4176085461405954 1104 100-101 -0.6900760480866737 1105 1 0.11208203363258917 1105 2 0.09480745839864824 1105 3 0.14898118490898327 1105 4 0.21888512994802056 1105 5 0.19547793012181103 1105 6 0.12037735401510474 1105 7 0.1541780081887012 1105 8 0.21009694055648254 1105 9 0.1289946844617944 1105 10-11 0.07785657787889022 1105 12-13 0.18065344350493717 1105 14-15 0.30277240361967017 1105 16-17 0.1876637011822453 1105 18-19 0.18209724122382198 1105 20-21 0.131578375179906 1105 22-23 0.19888596072932785 1105 24-25 0.1029854909329515 1105 26-27 0.15798155828296245 1105 28-29 0.15416747701118538 1105 30-31 0.24190866956875112 1105 32-33 0.1956881187895263 1105 34-35 0.1859987022278915 1105 36-37 0.08997502897932463 1105 38-39 0.1521164644004287 1105 40-41 0.08674733482136077 1105 42-43 0.03887509972634007 1105 44-45 0.03980030808610735 1105 46-47 -0.05456227263584168 1105 48-49 0.10852987729125374 1105 50-51 -0.07667309043325332 1105 52-53 -0.12077111140683172 1105 54-55 -0.009557892560991377 1105 56-57 -0.17896890066348448 1105 58-59 -0.11951909910616365 1105 60-61 0.04353654473391089 1105 62-63 -0.014548832959370372 1105 64-65 -0.03623383147136394 1105 66-67 -0.09068032492857725 1105 68-69 -0.12435310929882348 1105 70-71 -0.12206077065352616 1105 72-73 0.02198666620414258 1105 74-75 0.14850843246109235 1105 76-77 0.10926586812514927 1105 78-79 0.029053762744915446 1105 80-81 0.03880785184176716 1105 82-83 0.04288445433211052 1105 84-85 0.055019243676170504 1105 86-87 -0.03451367211741996 1105 88-89 -0.21760045786613702 1105 90-91 -0.16699760958713483 1105 92-93 -0.03881352869900212 1105 94-95 -0.20638471699946237 1105 96-97 -0.2075059221096005 1105 98-99 -0.2710267680570233 1105 100-101 -0.25443205796491597 1106 1 0.16386363764197398 1106 2 0.1329679595996609 1106 3 0.13381603006691734 1106 4 0.1969819354218103 1106 5 0.1568168294905874 1106 6 0.1719340821717097 1106 7 0.18308887857283906 1106 8 0.23029995904929734 1106 9 0.23126020125371838 1106 10-11 0.19198196836793358 1106 12-13 0.2907559553825365 1106 14-15 0.29549204788612826 1106 16-17 0.39057870813620355 1106 18-19 0.3946597087278292 1106 20-21 0.3345530028814636 1106 22-23 0.32258271922268733 1106 24-25 0.32268937813407916 1106 26-27 0.37909808121528954 1106 28-29 0.3261165777226225 1106 30-31 0.3698023348016548 1106 32-33 0.34745622308608404 1106 34-35 0.36233610481152567 1106 36-37 0.38952559954809374 1106 38-39 0.4905488935036644 1106 40-41 0.4700310379107506 1106 42-43 0.3585411376000138 1106 44-45 0.4751237927164951 1106 46-47 0.632148244822119 1106 48-49 0.5048243314516725 1106 50-51 0.390224503681587 1106 52-53 0.4083218439631864 1106 54-55 0.45253101074669644 1106 56-57 0.45834913967952673 1106 58-59 0.668701201883728 1106 60-61 0.5119020983367193 1106 62-63 0.5855073143873071 1106 64-65 0.49432135406553357 1106 66-67 0.3826342878480986 1106 68-69 0.43314635715815086 1106 70-71 0.3735142240110392 1106 72-73 0.41616574759345326 1106 74-75 0.3461843333610304 1106 76-77 0.4558187146667123 1106 78-79 0.4506057498883216 1106 80-81 0.37096470489757394 1106 82-83 0.48760313034329883 1106 84-85 0.43334780018804153 1106 86-87 0.4611993633771547 1106 88-89 0.5940454256882788 1106 90-91 0.6168383284387993 1106 92-93 0.4878278143684476 1106 94-95 0.2476595033330362 1106 96-97 0.4681298176818238 1106 98-99 0.44693844923464 1106 100-101 0.15173920863042056 1107 1 0.05512695445651872 1107 2 0.18284008366013182 1107 3 0.14973579768704326 1107 4 0.09880529707162111 1107 5 0.19374094357277727 1107 6 0.12653431629788514 1107 7 0.10437638977911945 1107 8 0.11937532310737708 1107 9 0.3174381269932809 1107 10-11 0.24128603694476425 1107 12-13 0.24770557358225176 1107 14-15 0.3791703264394215 1107 16-17 0.3911340724785646 1107 18-19 0.3193149411838405 1107 20-21 0.21625938266741684 1107 22-23 0.292174744718686 1107 24-25 0.31221715599646416 1107 26-27 0.24169007929841513 1107 28-29 0.16102361257453168 1107 30-31 0.23321367296573925 1107 32-33 0.22516692395182503 1107 34-35 0.18024817855416586 1107 36-37 0.3289081399896787 1107 38-39 0.34312707347216787 1107 40-41 0.10174071739344015 1107 42-43 0.10121098822020258 1107 44-45 0.057832975721730406 1107 46-47 0.1705572224339349 1107 48-49 0.1733144612972808 1107 50-51 0.16801937219629082 1107 52-53 0.15994148741319236 1107 54-55 0.26323603917992955 1107 56-57 0.06334955087250904 1107 58-59 0.07022911376456165 1107 60-61 -0.09222313175366992 1107 62-63 -0.005097281430302303 1107 64-65 -0.03181985874053339 1107 66-67 -0.13313673959624595 1107 68-69 -0.12391453696828592 1107 70-71 0.06008110784851084 1107 72-73 -0.006641654692373322 1107 74-75 0.11546649195155823 1107 76-77 0.06621286090317824 1107 78-79 0.05399235555628579 1107 80-81 0.03563632415521312 1107 82-83 0.14475347163347152 1107 84-85 -0.042122761948288456 1107 86-87 0.016131191642664078 1107 88-89 0.0043794666796479476 1107 90-91 0.17560541959875664 1107 92-93 0.19356695151298098 1107 94-95 0.0719009030542388 1107 96-97 0.31198106126137404 1107 98-99 0.29991919220450924 1107 100-101 0.48751685577405013 1108 1 0.011777697362582273 1108 2 0.06738344853520317 1108 3 0.10995565934793916 1108 4 0.07354264379761588 1108 5 0.07532807199233105 1108 6 0.18309539557572663 1108 7 0.1730678696301169 1108 8 0.1869370478186667 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-0.6697181523260163 2316 94-95 -0.5179040726064379 2316 96-97 -0.6360340023244255 2316 98-99 -0.6482240342154633 2316 100-101 -0.576511127864471 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5366.0 3 1319.0 4 1277.0 5 1450.0 6 1737.0 7 2031.0 8 2460.0 9 2884.0 10 3461.0 11 4453.0 12 5837.0 13 9588.0 14 8016.0 15 7938.0 16 9547.0 17 13450.0 18 13074.0 19 8321.0 20 8238.0 21 8911.0 22 9396.0 23 10070.0 24 11630.0 25 13017.0 26 15098.0 27 17751.0 28 21336.0 29 25489.0 30 30910.0 31 37971.0 32 48142.0 33 63547.0 34 89139.0 35 134397.0 36 225539.0 37 392593.0 38 429426.0 39 85268.0 40 51.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93886357038778 18.625078562510392 14.171859542914156 23.26419832418767 2 15.439121604939313 20.20487486087373 40.251712399068424 24.104291135118537 3 19.710355857836824 24.317702012504906 29.520656490730417 26.451285638927846 4 13.068745514713989 16.297476541532344 36.651395134856664 33.98238280889701 5 14.223396487928024 37.00292616413744 33.75225143636296 15.021425911571567 6 32.29219084095219 35.09896316803582 17.62181412140284 14.987031869609147 7 28.92919014933883 32.46065791404709 20.46412612408972 18.146025812524353 8 26.24262231659259 34.747595505011425 19.924412484479873 19.08536969391611 9 26.58025652039951 17.297022555173477 20.478113902614005 35.64460702181301 10-11 25.314857920363437 26.374495383957992 26.988777564124188 21.321869131554386 12-13 26.2919358887964 23.70313018782364 27.57261933979888 22.432314583581086 14-15 23.04914442509274 24.95897485637334 26.15434787803647 25.837532840497445 16-17 22.60554503584697 28.424886657011523 26.566027912959782 22.403540394181725 18-19 22.40145838054308 26.9846509046559 28.80771092846367 21.806179786337353 20-21 23.288374567404972 26.09977831789282 28.546576299247228 22.065270815454987 22-23 22.911861621446516 26.105729835401014 28.44553026046649 22.536878282685976 24-25 22.91361580117474 26.22673268301326 28.498278109562573 22.36137340624943 26-27 22.64294374383563 26.461076626739416 28.590661180290784 22.305318449134166 28-29 22.30489585849703 26.70855593392696 28.756964163331585 22.229584044244426 30-31 22.428567572157913 26.378033129484386 28.568275149962265 22.625124148395432 32-33 22.33697180326394 26.489157697022865 28.52102102102102 22.652849478692175 34-35 23.007277366496833 26.424536812235605 28.239045270839046 22.329140550428516 36-37 22.833116025096913 26.206808753038384 28.333672539305315 22.626402682559384 38-39 22.631720644037205 26.484300083363387 28.539853851619938 22.344125420979466 40-41 22.683863548257673 26.386104281652212 28.511327810453217 22.418704359636894 42-43 22.584499988287117 26.40335163459642 28.126500272218706 22.88564810489776 44-45 22.661704194748985 26.64411048932811 27.921045664263143 22.77313965165976 46-47 22.749780668821774 26.728510343285755 27.622461319238926 22.899247668653537 48-49 22.971451188214044 26.745974097247256 27.59311951249051 22.68945520204819 50-51 22.704436637330712 26.919036040374433 27.673163331957713 22.703363990337145 52-53 22.982421621932904 26.954811562643876 27.401815170571325 22.66095164485189 54-55 22.446995214765415 26.674055922384028 27.650775533152583 23.228173329697977 56-57 22.745460784291595 27.186758560905517 27.370629812652236 22.697150842150652 58-59 22.962931824202723 26.598906634533975 27.56023539774223 22.877926143521073 60-61 22.924616605097683 26.521234320213814 27.9309803160676 22.623168758620903 62-63 22.801141049035873 26.684777301838963 27.84902435679118 22.665057292333987 64-65 22.56847520709685 26.625185190203887 27.726191766714546 23.080147835984715 66-67 22.526874677441757 26.91324326735547 27.43151036872995 23.128371686472825 68-69 22.406030578128057 26.83711176261474 27.68041459801087 23.07644306124633 70-71 22.51235581354968 26.89904019221566 27.51360515887861 23.074998835356052 72-73 22.665282565777495 26.6490708559339 27.533086720959783 23.15255985732882 74-75 22.462052222150383 27.04506357733278 27.359188143562214 23.133696056954626 76-77 22.63189672668437 26.761965598331432 27.380989009158895 23.22514866582531 78-79 22.622897800776194 26.82565941719779 27.468313205954004 23.08312957607201 80-81 22.46979221787138 27.17039545630931 27.4185487000388 22.941263625780508 82-83 22.683857929342892 26.963279925823162 27.28951123166572 23.06335091316822 84-85 22.589243905351065 26.820124626612696 27.1788417577337 23.411789710302543 86-87 22.713727836873176 26.69779113206775 27.456125371002592 23.13235566005648 88-89 22.69861702758323 26.627963764372442 27.297684883284358 23.37573432475998 90-91 22.50038186247812 26.784130670657802 27.513269191730537 23.20221827513354 92-93 22.784631442184963 26.645460569165486 27.3902242845483 23.179683704101254 94-95 22.63720682832517 26.577717597528146 27.33015580109499 23.454919773051696 96-97 22.725375967764432 26.43875112015327 27.427930453657133 23.40794245842516 98-99 22.611144173209762 26.487879133499487 27.163258761048354 23.737717932242397 100-101 22.7668510146289 25.967290468856564 27.748621342209738 23.5172371743048 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1635.0 1 933.0 2 264.0 3 303.5 4 274.0 5 269.5 6 426.5 7 854.0 8 1432.5 9 1795.0 10 1871.0 11 1886.5 12 2000.5 13 2219.0 14 2399.5 15 2565.0 16 2739.5 17 2795.0 18 2822.5 19 2928.5 20 3105.0 21 3418.0 22 3940.0 23 4777.0 24 5816.0 25 7061.5 26 8844.0 27 10903.5 28 12941.0 29 15251.0 30 17823.5 31 20687.0 32 23570.0 33 26201.0 34 29728.5 35 32752.5 36 35836.0 37 39848.5 38 42842.0 39 44619.0 40 46471.0 41 50523.5 42 55313.0 43 59212.0 44 62763.0 45 65593.5 46 66421.0 47 69856.5 48 81754.5 49 83715.5 50 75873.0 51 72362.5 52 68280.5 53 66064.0 54 64085.0 55 58374.0 56 51442.0 57 45220.5 58 39683.0 59 34680.0 60 29466.5 61 24328.5 62 20089.0 63 15773.0 64 11937.5 65 9299.0 66 7143.5 67 5803.0 68 4351.5 69 3017.0 70 2334.0 71 1744.5 72 1394.0 73 1146.0 74 874.5 75 708.5 76 517.0 77 308.0 78 184.5 79 118.5 80 78.5 81 54.0 82 38.5 83 27.5 84 20.0 85 22.0 86 16.0 87 10.0 88 11.5 89 8.5 90 7.0 91 7.5 92 6.0 93 6.5 94 6.5 95 4.0 96 4.5 97 6.5 98 4.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34059348541228496 2 0.11611524564525696 3 0.1093179816282874 4 0.11751963903719283 5 0.13336119649822933 6 0.08634210573621671 7 0.09488081755918676 8 0.10066691833396249 9 0.08740944471408797 10-11 0.0869600388286685 12-13 0.09125748260799223 14-15 0.09243717305721835 16-17 0.08179187114634452 18-19 0.09645373815815492 20-21 0.10707095220119003 22-23 0.11510408240306316 24-25 0.1283896438907764 26-27 0.12749083211993745 28-29 0.12282824605871039 30-31 0.11313793165435296 32-33 0.1071833036725449 34-35 0.33303784896367 36-37 0.49628453684229445 38-39 0.47002238041309385 40-41 0.4485632493843139 42-43 0.4816788455661615 44-45 0.48867272465800216 46-47 0.49625644897445575 48-49 0.5057782361717809 50-51 0.4948520555825199 52-53 0.4993742023045534 54-55 0.4851898290460012 56-57 0.46266335903934996 58-59 0.44342316956982863 60-61 0.4035945729745276 62-63 0.4732805730823851 64-65 0.5042334034406515 66-67 0.5128844667349763 68-69 0.5253835679232055 70-71 0.5168448561002355 72-73 0.5090926045767495 74-75 0.5153842869726222 76-77 0.49375662873681 78-79 0.47255028851857844 80-81 0.47415129698538533 82-83 0.4879143522263567 84-85 0.48740877060525983 86-87 0.49128489636700284 88-89 0.5023796041632962 90-91 0.5169572075715904 92-93 0.5110025795897823 94-95 0.5141203329198799 96-97 0.5148506174836867 98-99 0.5083904078807816 100-101 0.4965935033885204 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1780128.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.4189742718767 #Duplication Level Percentage of deduplicated Percentage of total 1 78.47138576062049 45.057464800675795 2 12.600330474357005 14.469961026484976 3 4.007827960646372 6.903761116753862 4 1.7196131890928015 3.9495370184839764 5 0.8731836900576537 2.5068655917021383 6 0.5029571990806385 1.7327571884319808 7 0.327224594335153 1.3152230394278808 8 0.24366346039844294 1.1192724770891715 9 0.17043081839318788 0.8807366498808011 >10 0.9432479413589016 9.90041976667648 >50 0.07939796067426133 3.1965247047291387 >100 0.05504657351304646 5.762876018928333 >500 0.004513057995088757 1.7391912658036741 >1k 0.0010792095205647027 1.1543344743815984 >5k 9.810995641497297E-5 0.3110748605502507 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5522 0.31020241241079294 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4487 0.2520605259846483 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2955 0.16599929892681875 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2864 0.16088730698017223 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1235147135486887E-4 0.0 10-11 0.0 0.0 0.0 1.1235147135486887E-4 0.0 12-13 0.0 0.0 0.0 1.1235147135486887E-4 0.0 14-15 0.0 0.0 0.0 1.685272070323033E-4 0.0 16-17 0.0 0.0 0.0 2.5279081054845493E-4 0.0 18-19 0.0 0.0 0.0 2.8087867838717215E-4 0.0 20-21 0.0 0.0 0.0 3.9323014974204103E-4 0.0 22-23 0.0 0.0 0.0 7.302845638066476E-4 0.0 24-25 0.0 0.0 0.0 0.0010954268457099713 0.0 26-27 0.0 0.0 0.0 0.0016571842024843157 0.0 28-29 2.8087867838717217E-5 0.0 0.0 0.004128916572291431 0.0 30-31 5.6175735677434434E-5 0.0 0.0 0.010926180589260998 0.0 32-33 1.1235147135486887E-4 0.0 0.0 0.023284842438296573 5.6175735677434434E-5 34-35 1.1235147135486887E-4 0.0 0.0 0.04252503190781787 5.6175735677434434E-5 36-37 1.685272070323033E-4 0.0 0.0 0.07645517625698826 5.6175735677434434E-5 38-39 1.685272070323033E-4 0.0 0.0 0.12496292401445289 5.6175735677434434E-5 40-41 1.685272070323033E-4 0.0 0.0 0.17965000269643533 5.6175735677434434E-5 42-43 1.685272070323033E-4 0.0 0.0 0.23714586816228944 8.426360351615165E-5 44-45 1.685272070323033E-4 0.0 0.0 0.30281530316921035 1.1235147135486887E-4 46-47 1.9661507487102052E-4 0.0 0.0 0.3868542037426522 1.1235147135486887E-4 48-49 2.2470294270973774E-4 0.0 0.0 0.4785330043682252 1.1235147135486887E-4 50-51 2.2470294270973774E-4 0.0 0.0 0.5771775962178001 1.1235147135486887E-4 52-53 2.2470294270973774E-4 0.0 0.0 0.6754570457854716 1.1235147135486887E-4 54-55 2.2470294270973774E-4 0.0 0.0 0.7869377932373403 1.1235147135486887E-4 56-57 2.2470294270973774E-4 0.0 0.0 0.9234448309335059 1.1235147135486887E-4 58-59 2.8087867838717215E-4 0.0 0.0 1.0744452084344496 1.4043933919358608E-4 60-61 3.0896654622588937E-4 0.0 0.0 1.2451632691581729 1.685272070323033E-4 62-63 3.370544140646066E-4 0.0 0.0 1.419757455643639 1.685272070323033E-4 64-65 3.370544140646066E-4 0.0 0.0 1.598958052454655 1.685272070323033E-4 66-67 3.370544140646066E-4 0.0 0.0 1.808437370795808 1.685272070323033E-4 68-69 3.370544140646066E-4 0.0 0.0 2.0525209423142607 1.685272070323033E-4 70-71 3.370544140646066E-4 0.0 0.0 2.3180074691258157 1.685272070323033E-4 72-73 3.370544140646066E-4 0.0 0.0 2.6013017041471174 1.685272070323033E-4 74-75 3.370544140646066E-4 0.0 0.0 2.898752224559133 1.685272070323033E-4 76-77 3.370544140646066E-4 0.0 0.0 3.2158361645904114 1.685272070323033E-4 78-79 3.370544140646066E-4 0.0 0.0 3.5813155009077997 1.685272070323033E-4 80-81 3.370544140646066E-4 0.0 0.0 3.9708942278308075 1.685272070323033E-4 82-83 3.370544140646066E-4 0.0 0.0 4.3801344622409175 1.685272070323033E-4 84-85 3.370544140646066E-4 0.0 0.0 4.804598321019612 1.685272070323033E-4 86-87 3.370544140646066E-4 0.0 0.0 5.2519818799547 1.685272070323033E-4 88-89 3.370544140646066E-4 0.0 0.0 5.728548733574215 1.685272070323033E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8115 0.0 23.68944 1 TACATGG 8220 0.0 22.818893 2 GTATCAA 11795 0.0 21.932434 1 ACATGGG 8355 0.0 21.769878 3 GAGTACT 4945 0.0 21.020437 12-13 GTACTTT 5285 0.0 19.534546 14-15 ATCAACG 13400 0.0 19.017294 3 TCAACGC 13520 0.0 18.91857 4 GGTATCA 9035 0.0 18.755486 1 AGTACTT 5265 0.0 18.753452 12-13 CAACGCA 13600 0.0 18.737627 5 TATCAAC 13680 0.0 18.662674 2 CATGGGG 4040 0.0 18.524517 4 AACGCAG 13785 0.0 18.481483 6 CTAGGCG 130 0.0076884907 18.21788 4 GTACCTG 1550 0.0 18.067936 1 TACCTGG 1425 0.0 17.949408 2 CATGGGA 4625 0.0 17.615213 4 ACGCAGA 14960 0.0 16.967064 7 AGAGTAC 11220 0.0 16.839972 10-11 >>END_MODULE