FastQCFastQC Report
Fri 27 May 2016
SRR939117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1780128
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56950.3199208146829891No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47590.26734032608891045No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30850.1733021445648852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29800.1674036923187546No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG78950.022.977951
TACATGG79800.022.3554632
GAGTACT51550.021.9990812-13
ACATGGG80150.021.6658763
GTATCAA121100.021.5464061
GTACTTT56950.020.07976514-15
AGTACTT54950.019.7743912-13
GGTATCA93800.019.6447531
CATGGGG37850.019.4296284
ATCAACG137500.018.8748423
TCAACGC137950.018.8476664
TATCAAC139000.018.7394242
CAACGCA138950.018.643735
CGTAAGA1559.701308E-418.3661562
AACGCAG141750.018.308936
CATGGGA43600.017.7378034
AGAGTAC111700.017.14058510-11
ACTTTTT67700.016.995516-17
ACGCAGA153650.016.8600487
TACTTTT65150.016.78772414-15
Produced by FastQC (version 0.11.5)