##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939117_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1780128 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.56986463894731 34.0 31.0 34.0 31.0 34.0 2 32.772846671700016 34.0 31.0 34.0 31.0 34.0 3 32.864805227489256 34.0 31.0 34.0 31.0 34.0 4 36.29991438817883 37.0 37.0 37.0 35.0 37.0 5 36.261811510183534 37.0 37.0 37.0 35.0 37.0 6 36.300092465260924 37.0 37.0 37.0 35.0 37.0 7 36.255097948012725 37.0 37.0 37.0 35.0 37.0 8 36.24452960685973 37.0 37.0 37.0 35.0 37.0 9 38.00562880871488 39.0 38.0 39.0 35.0 39.0 10-11 37.949562896600696 39.0 38.0 39.0 35.0 39.0 12-13 37.88537172607812 39.0 38.0 39.0 35.0 39.0 14-15 39.28447841952938 41.0 39.0 41.0 36.0 41.0 16-17 39.22914784779522 40.5 39.0 41.0 36.0 41.0 18-19 39.24922365133294 40.5 39.0 41.0 36.0 41.0 20-21 39.315089139657374 41.0 39.0 41.0 36.0 41.0 22-23 39.28389419187834 41.0 39.0 41.0 36.0 41.0 24-25 39.22839874435995 41.0 39.0 41.0 36.0 41.0 26-27 39.108027344101096 41.0 39.0 41.0 35.5 41.0 28-29 38.96548871766525 40.0 38.5 41.0 35.0 41.0 30-31 38.79676545731543 40.0 38.0 41.0 35.0 41.0 32-33 38.68271691698574 40.0 38.0 41.0 35.0 41.0 34-35 38.64092638282191 40.0 38.0 41.0 35.0 41.0 36-37 38.550644953621315 40.0 38.0 41.0 35.0 41.0 38-39 38.4385928989376 40.0 38.0 41.0 34.0 41.0 40-41 38.281519362652574 40.0 38.0 41.0 34.0 41.0 42-43 38.13365387208111 40.0 38.0 41.0 34.0 41.0 44-45 37.93154902344101 40.0 38.0 41.0 33.0 41.0 46-47 37.65335751136997 40.0 37.5 41.0 33.0 41.0 48-49 37.35818435528232 40.0 37.0 41.0 32.5 41.0 50-51 37.11747891162882 40.0 37.0 41.0 32.0 41.0 52-53 36.89223949064337 40.0 36.0 41.0 31.0 41.0 54-55 36.6566996867641 39.5 36.0 41.0 31.0 41.0 56-57 36.39770623236082 39.0 35.5 41.0 31.0 41.0 58-59 36.1118624615758 39.0 35.0 40.5 30.0 41.0 60-61 35.76403859722447 38.5 35.0 40.0 29.0 41.0 62-63 35.38719603309425 38.0 35.0 40.0 28.5 41.0 64-65 34.987091377698675 37.0 34.0 40.0 28.0 41.0 66-67 34.63098805254454 37.0 34.0 39.5 28.0 41.0 68-69 34.17726084865807 36.0 34.0 39.0 27.5 40.5 70-71 33.745655649481385 36.0 33.5 39.0 26.0 40.0 72-73 33.289692651314965 35.0 33.0 38.0 26.0 40.0 74-75 32.80946763378813 35.0 33.0 37.0 25.5 39.0 76-77 30.8132381491668 33.5 30.0 35.5 21.5 37.5 78-79 31.79105996872135 34.5 31.5 36.0 24.0 38.5 80-81 31.818036680508367 35.0 32.0 36.0 24.0 37.5 82-83 31.53794979911557 35.0 32.0 35.5 23.5 37.0 84-85 31.179857572039765 35.0 32.0 35.0 22.0 37.0 86-87 30.655905642740297 34.0 31.0 35.0 19.0 36.0 88-89 30.140744092559636 34.0 31.0 35.0 15.0 36.0 90-91 29.720481055294897 34.0 30.0 35.0 9.0 35.5 92-93 29.463021198475616 34.0 30.0 35.0 4.5 35.0 94-95 29.174859055079185 34.0 30.0 35.0 2.0 35.0 96-97 28.668967343921338 34.0 29.0 35.0 2.0 35.0 98-99 27.960844107839435 34.0 29.0 35.0 2.0 35.0 100-101 24.377122038415216 30.0 14.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15716958536899028 1101 2 0.1953222646643198 1101 3 0.23603160060726935 1101 4 0.1789125690676414 1101 5 0.22538817109349907 1101 6 0.1920375629966813 1101 7 0.19796929727155543 1101 8 0.288740840082383 1101 9 0.3141280693251005 1101 10-11 0.28974430815683405 1101 12-13 0.2639365308053172 1101 14-15 0.3604080342646796 1101 16-17 0.3318908022196396 1101 18-19 0.2947874733628666 1101 20-21 0.39016043978077164 1101 22-23 0.32973683612701166 1101 24-25 0.29473496540472155 1101 26-27 0.34217744831878605 1101 28-29 0.4244616810023203 1101 30-31 0.32156046548215755 1101 32-33 0.32542627199977403 1101 34-35 0.5604483835189669 1101 36-37 0.555652852024707 1101 38-39 0.47271934653880265 1101 40-41 0.5314828836275041 1101 42-43 0.5921654729474355 1101 44-45 0.5892286773323647 1101 46-47 0.6901799603230501 1101 48-49 0.5553638203059492 1101 50-51 0.4439716036390351 1101 52-53 0.45584823253665974 1101 54-55 0.5989390411074282 1101 56-57 0.47771235861576855 1101 58-59 0.3797756193360655 1101 60-61 0.3753857911593599 1101 62-63 0.35453238306948975 1101 64-65 0.3046582802264979 1101 66-67 0.34423255877635484 1101 68-69 0.4152210233889875 1101 70-71 0.3010019942526938 1101 72-73 0.21557825657232854 1101 74-75 0.14236550651769164 1101 76-77 0.17064717250691075 1101 78-79 0.403661929803075 1101 80-81 0.1705138401300168 1101 82-83 0.4018334469944911 1101 84-85 0.2451782729063261 1101 86-87 0.26098351034968914 1101 88-89 0.4879044727218407 1101 90-91 0.4582015511021531 1101 92-93 0.4794449854618108 1101 94-95 0.22812680010182973 1101 96-97 0.3586733669290503 1101 98-99 0.42138840453540993 1101 100-101 0.0646244528464841 1102 1 0.23195609479182622 1102 2 0.1568889755883589 1102 3 0.18975869160186676 1102 4 0.09191443186536219 1102 5 0.0798543989530387 1102 6 0.156348618642987 1102 7 0.11498049566034751 1102 8 0.09733795897739839 1102 9 0.19201511498773982 1102 10-11 0.19542542576899535 1102 12-13 0.20256806409887673 1102 14-15 0.21266167054221086 1102 16-17 0.1749810440510018 1102 18-19 0.2163848348702615 1102 20-21 0.20286327452352992 1102 22-23 0.2220007418870651 1102 24-25 0.2132479509825842 1102 26-27 0.12483810008196627 1102 28-29 0.16116577533532706 1102 30-31 0.28894052606877807 1102 32-33 0.23462609984470362 1102 34-35 0.26059174915076255 1102 36-37 0.22361061254635217 1102 38-39 0.165568346925987 1102 40-41 0.2920296671026108 1102 42-43 0.29182299069054807 1102 44-45 0.25852246761890285 1102 46-47 0.26698920883954 1102 48-49 0.17735609160640564 1102 50-51 0.1866103538197521 1102 52-53 0.2534027496965052 1102 54-55 0.2713761986874772 1102 56-57 0.22306450382425425 1102 58-59 0.26067724832857664 1102 60-61 0.24446463685623598 1102 62-63 0.3808099199889483 1102 64-65 0.3805654178491906 1102 66-67 0.3303683419407193 1102 68-69 0.22203739241237486 1102 70-71 0.19100553373453266 1102 72-73 0.24686175078867478 1102 74-75 0.09800794739754792 1102 76-77 0.24695333270086905 1102 78-79 0.2515790776786382 1102 80-81 0.2156596817373746 1102 82-83 0.2280257787818627 1102 84-85 0.22506678753701692 1102 86-87 0.41668996932244085 1102 88-89 0.3028026586127659 1102 90-91 0.48094833025676564 1102 92-93 0.3693423820316717 1102 94-95 0.3084518508260956 1102 96-97 0.09145910530983059 1102 98-99 0.17527456449672485 1102 100-101 -0.32171019768368936 1103 1 -0.013761826595420246 1103 2 0.01092129977555345 1103 3 0.0473001981025547 1103 4 0.024288618173962107 1103 5 0.11424727946479862 1103 6 0.1312590301951886 1103 7 0.14614594128751435 1103 8 0.07619203068995972 1103 9 0.11814423489076376 1103 10-11 0.08739357424224892 1103 12-13 0.09501530377792022 1103 14-15 0.1411770022646337 1103 16-17 0.17037441330009528 1103 18-19 0.175427158880602 1103 20-21 0.1902376939469832 1103 22-23 0.14551455434375526 1103 24-25 0.05352328373393078 1103 26-27 0.10787397083023365 1103 28-29 0.22664371704374986 1103 30-31 0.28262331463682955 1103 32-33 0.32095656666923844 1103 34-35 0.18427577563903697 1103 36-37 0.30537825813460984 1103 38-39 0.30812330174189384 1103 40-41 0.25546741177271315 1103 42-43 0.20256009984134948 1103 44-45 0.3219713053404192 1103 46-47 0.34269124626331404 1103 48-49 0.40767697821451776 1103 50-51 0.41894808578911835 1103 52-53 0.46225717510055375 1103 54-55 0.4266254627680155 1103 56-57 0.3644771958827633 1103 58-59 0.388010808346003 1103 60-61 0.4226658664164802 1103 62-63 0.3307097207963281 1103 64-65 0.3107939427466633 1103 66-67 0.31082407343100016 1103 68-69 0.2858781042325873 1103 70-71 0.28081343102251566 1103 72-73 0.2969631557477541 1103 74-75 0.1648060024158724 1103 76-77 0.2678665480620097 1103 78-79 0.20455639640772816 1103 80-81 0.17460253001497605 1103 82-83 0.1831463473891688 1103 84-85 0.22685011612766814 1103 86-87 0.3817548958269583 1103 88-89 0.28885937625923575 1103 90-91 0.4027493514605709 1103 92-93 0.13644437058171377 1103 94-95 0.051645701095626606 1103 96-97 0.056273232391184536 1103 98-99 -0.17669810961997356 1103 100-101 -0.2873392219292441 1104 1 -0.03702699546666821 1104 2 0.0772249097579163 1104 3 0.0727129875029604 1104 4 -0.020110990878180246 1104 5 0.08326961126137178 1104 6 0.0546883248862855 1104 7 0.06062452557857512 1104 8 0.04746294121506622 1104 9 0.0565660186060839 1104 10-11 0.06342464422631622 1104 12-13 0.08652028019219671 1104 14-15 0.09189879446515903 1104 16-17 -0.007153116468820997 1104 18-19 0.0027193028399281616 1104 20-21 0.07315544876232849 1104 22-23 0.06140341075840894 1104 24-25 0.051349932613099725 1104 26-27 0.08281807062878954 1104 28-29 0.012123649539240944 1104 30-31 0.08174694246955028 1104 32-33 -0.008069544185218547 1104 34-35 0.09192789646832722 1104 36-37 0.17544643045336983 1104 38-39 0.210385943908328 1104 40-41 0.28591377272067575 1104 42-43 0.2183184176970414 1104 44-45 0.17001114493866254 1104 46-47 0.10103168988228362 1104 48-49 0.07960997025414684 1104 50-51 0.03896499334125991 1104 52-53 -0.05453233058924667 1104 54-55 -0.024138166196067345 1104 56-57 0.14563929939594544 1104 58-59 0.1306642333823831 1104 60-61 0.09519321548201276 1104 62-63 -0.013282166407044826 1104 64-65 -0.07595884872791459 1104 66-67 0.18665491620019026 1104 68-69 -0.0015306405545132407 1104 70-71 0.11929818594158093 1104 72-73 0.09490337278038652 1104 74-75 0.02358296257627046 1104 76-77 0.0894046639070254 1104 78-79 0.2154052780894311 1104 80-81 0.07644012637702247 1104 82-83 0.28140401314131935 1104 84-85 0.13998520998343977 1104 86-87 0.046408291007992375 1104 88-89 0.1995300293998632 1104 90-91 0.24995402712155368 1104 92-93 0.39332799131200247 1104 94-95 0.1794012314050839 1104 96-97 0.24314135976979045 1104 98-99 0.2758069500750011 1104 100-101 -0.18894563579972257 1105 1 0.0070565027088065335 1105 2 0.0339262035127561 1105 3 0.019714191567295813 1105 4 -0.00642090229344916 1105 5 0.022447468764042355 1105 6 0.009386940212301909 1105 7 1.893036534283965E-5 1105 8 0.030982310678517422 1105 9 0.11257343957400678 1105 10-11 0.038001839493610134 1105 12-13 0.006462288522357085 1105 14-15 0.06439421779206356 1105 16-17 0.051668651294200174 1105 18-19 0.0732776262417758 1105 20-21 0.07651193166584846 1105 22-23 0.030074701195921705 1105 24-25 -0.09671081734696685 1105 26-27 -2.0480316642590424E-5 1105 28-29 0.007301249028138557 1105 30-31 -0.007551167886632015 1105 32-33 0.05353201495522342 1105 34-35 0.10626839480352146 1105 36-37 0.0834959998160727 1105 38-39 0.10485062673566148 1105 40-41 0.164399262516838 1105 42-43 0.19022793471980748 1105 44-45 0.1615243343576438 1105 46-47 0.18011829036636584 1105 48-49 0.24220012149937276 1105 50-51 0.1264685256728484 1105 52-53 0.08838964481343936 1105 54-55 0.13128672721967405 1105 56-57 0.011694053607101296 1105 58-59 0.15353147300347558 1105 60-61 0.26600755613392835 1105 62-63 0.3537186383502373 1105 64-65 0.11543823448767654 1105 66-67 0.14269274593566905 1105 68-69 -0.04462969327533273 1105 70-71 0.19282827526349422 1105 72-73 0.17358989831760852 1105 74-75 0.16516418777315778 1105 76-77 0.20234251944388149 1105 78-79 0.4017234828049432 1105 80-81 0.20902081711678377 1105 82-83 0.21023597859204557 1105 84-85 0.31060757609271405 1105 86-87 0.24280592711026117 1105 88-89 0.3537262857427024 1105 90-91 0.24620514825287998 1105 92-93 0.33475271886173275 1105 94-95 0.06144208973094223 1105 96-97 -0.017100866029132078 1105 98-99 -0.08844785082436246 1105 100-101 -0.3075344539737195 1106 1 0.032444556787872614 1106 2 0.026907785177783694 1106 3 0.07856864246200246 1106 4 -0.011288318641774708 1106 5 0.02906662503556845 1106 6 0.04524855132603989 1106 7 0.0342713184179928 1106 8 0.025858507211417248 1106 9 -0.03998513199165643 1106 10-11 0.030118651756644965 1106 12-13 0.052174930126106744 1106 14-15 0.059722314525920694 1106 16-17 -0.011743474527428077 1106 18-19 -0.018505560492549478 1106 20-21 0.0053396194694173005 1106 22-23 0.10055125896251838 1106 24-25 0.22313390372818986 1106 26-27 0.13109861364851128 1106 28-29 0.09665144375343715 1106 30-31 0.12598928040409874 1106 32-33 0.1918188476862639 1106 34-35 0.14836690904732563 1106 36-37 0.26181467324907004 1106 38-39 0.25666044765140583 1106 40-41 0.2424265283559066 1106 42-43 0.27378596486463636 1106 44-45 0.253674555724281 1106 46-47 0.218913313878069 1106 48-49 0.2895105275220331 1106 50-51 0.22167408655190002 1106 52-53 0.2670291602274588 1106 54-55 0.29121624940861324 1106 56-57 0.30498372951372943 1106 58-59 0.32931474281295436 1106 60-61 0.24623584809440047 1106 62-63 0.3223482778470341 1106 64-65 0.4520800717090694 1106 66-67 0.4905167855940249 1106 68-69 0.3601864098409848 1106 70-71 0.44972172278219347 1106 72-73 0.2956953378782856 1106 74-75 0.280114779540412 1106 76-77 0.3690902918122241 1106 78-79 0.41182785671189137 1106 80-81 0.3764312212774179 1106 82-83 0.3942075224865249 1106 84-85 0.44973106874414626 1106 86-87 0.28146649061763895 1106 88-89 0.45217267290443885 1106 90-91 0.6761627656001217 1106 92-93 0.41629689264350134 1106 94-95 0.3456640407652998 1106 96-97 0.3827140768996671 1106 98-99 0.4572536902197797 1106 100-101 0.2316247395097477 1107 1 -0.041901164114641176 1107 2 -0.0016482979908190032 1107 3 -0.04441765415129595 1107 4 0.02563478014911169 1107 5 0.0717261196844774 1107 6 0.09633902494928037 1107 7 0.0330661676421542 1107 8 0.0642456560602298 1107 9 0.03712522712702793 1107 10-11 0.1327783088521599 1107 12-13 0.021043052923566563 1107 14-15 0.13482391980300434 1107 16-17 0.13470924995601763 1107 18-19 0.14076673961942987 1107 20-21 0.14403247252934648 1107 22-23 0.17390047145182308 1107 24-25 -0.004517757948313772 1107 26-27 0.08656895242076246 1107 28-29 0.18188057977099703 1107 30-31 0.24808212067136282 1107 32-33 0.21287395871344472 1107 34-35 0.21385015217202863 1107 36-37 0.26612712305560393 1107 38-39 0.3151315813967486 1107 40-41 0.36876937801461906 1107 42-43 0.40430013883885607 1107 44-45 0.16529192492176747 1107 46-47 0.32637580647913467 1107 48-49 0.20472355086662475 1107 50-51 0.2657473743392629 1107 52-53 0.1879907629257005 1107 54-55 0.22259575615979088 1107 56-57 0.3178048280792254 1107 58-59 0.22900599272792732 1107 60-61 0.26271808709600464 1107 62-63 0.20958494935664618 1107 64-65 0.30491613566836406 1107 66-67 0.3676370945674776 1107 68-69 0.317958921641214 1107 70-71 0.3516197083427244 1107 72-73 0.28599930594128153 1107 74-75 0.3581850965854301 1107 76-77 0.25934085555271835 1107 78-79 0.45954299440063195 1107 80-81 0.40293793895231644 1107 82-83 0.42414998592842323 1107 84-85 0.5803731694224616 1107 86-87 0.5040274817043979 1107 88-89 0.48383554745808866 1107 90-91 0.5329813096926976 1107 92-93 0.5494548851689274 1107 94-95 0.4764985391454033 1107 96-97 0.4906451264367462 1107 98-99 0.7158311249644704 1107 100-101 0.2984227848709331 1108 1 -0.068642100016163 1108 2 -0.059307567654755644 1108 3 -0.0419128594323368 1108 4 0.030394810753769264 1108 5 -0.016413851585021177 1108 6 -0.040601370603113196 1108 7 -0.04641322435492867 1108 8 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2316 82-83 -0.15904976378621427 2316 84-85 -0.27320563252220964 2316 86-87 -0.35209057059223525 2316 88-89 -0.6651702032198941 2316 90-91 -0.8153439776476006 2316 92-93 -0.7630274004816364 2316 94-95 -0.3993097385474478 2316 96-97 -0.8351606731831787 2316 98-99 -0.6125762640294816 2316 100-101 -0.1147753540962988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 12.0 5 28.0 6 59.0 7 153.0 8 326.0 9 584.0 10 1063.0 11 1679.0 12 2687.0 13 3955.0 14 4811.0 15 6298.0 16 8739.0 17 12760.0 18 12899.0 19 8401.0 20 7580.0 21 8096.0 22 8176.0 23 9059.0 24 10172.0 25 12086.0 26 14602.0 27 17176.0 28 21742.0 29 25994.0 30 32441.0 31 40347.0 32 51616.0 33 67585.0 34 94191.0 35 140691.0 36 237126.0 37 433173.0 38 435893.0 39 47919.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.23450538956452 19.047224434872266 14.519325031808606 23.198945143754603 2 15.622865322044257 20.483358500062916 39.67939384134175 24.21438233655108 3 19.71785174998652 24.226460119721725 29.648879181721767 26.406808948569992 4 13.286909705369501 16.66200408060544 36.24318026568876 33.8079059483363 5 14.24751478545363 37.15165426306422 33.66139962968955 14.93943132179259 6 32.442442341224904 35.36919817001923 17.282577432634056 14.905782056121808 7 29.033136942961406 32.51429110715634 20.26612693019828 18.186445019683976 8 26.395461449963147 34.86457153642884 19.66813622391199 19.071830789696023 9 26.53342905678693 17.19078628053713 20.318763594528033 35.95702106814791 10-11 25.38688229161049 26.462956596379588 26.77790585845512 21.3722552535548 12-13 26.207048032501035 23.688043781121358 27.570039907242627 22.534868279134983 14-15 22.94876785911906 25.022709232503782 26.079833134188675 25.948689774188495 16-17 22.61807790141655 28.472593612928744 26.4984023333573 22.410926152297403 18-19 22.37766676825115 26.9727589737252 28.80588609523756 21.843688162786094 20-21 23.179456758165706 26.087871209261355 28.65333279404627 22.079339238526668 22-23 22.804034316633413 26.08298392025742 28.55946313972928 22.553518623379894 24-25 22.872381810536773 26.199379423336143 28.623431529979605 22.304807236147482 26-27 22.591601389581733 26.456263229564115 28.665881319293707 22.28625406156045 28-29 22.2537648978051 26.74403188984163 28.784194170306854 22.218009042046415 30-31 22.39934002854933 26.397256972591638 28.660642208174142 22.5427607906849 32-33 22.29645845691995 26.495229556526272 28.54918298010031 22.65912900645347 34-35 22.994902369146903 26.443155952334468 28.29672498215534 22.265216696363286 36-37 22.805512701898785 26.207641958142553 28.428630469554015 22.55821487040464 38-39 22.61218001844078 26.497302150694114 28.6073164167347 22.283201414130403 40-41 22.66759743187459 26.354972688034 28.59937159302713 22.378058287064285 42-43 22.52199284981321 26.46091597336672 28.147083617633832 22.870007559186238 44-45 22.60820739776987 26.63668481756688 28.011850049183266 22.743257735479983 46-47 22.688299074156777 26.76406530618665 27.692408377698648 22.855227241957927 48-49 22.96385990901147 26.811758457110486 27.576428370197537 22.64795326368051 50-51 22.62058342482964 26.952747459239557 27.641855789462156 22.784813326468655 52-53 22.980130069371455 26.981024387585826 27.38402934172674 22.654816201315974 54-55 22.41451176544608 26.723555834187206 27.652168832803035 23.209763567563684 56-57 22.66637567635586 27.228659961530855 27.36373451796725 22.741229844146037 58-59 22.93406664400819 26.66086160259093 27.486341714160517 22.91873003924036 60-61 22.87265689301134 26.5882283540876 27.924020551081348 22.61509420181971 62-63 22.705400312181453 26.677469706685947 27.831479317065856 22.785650664066743 64-65 22.47729395324668 26.6709642082201 27.682824580252372 23.168917258280842 66-67 22.43700389399065 26.990807283276514 27.42146922236195 23.150719600370888 68-69 22.324693655419402 26.85534918015519 27.6509954004424 23.168961763983006 70-71 22.423808849700695 26.950814772870263 27.450694556795916 23.174681820633122 72-73 22.550569397256826 26.670610203311224 27.52987425623326 23.248946143198694 74-75 22.363672724658002 27.00522097287386 27.278824893490807 23.352281408977333 76-77 22.430210413860372 26.703628791045762 27.476033032259515 23.390127762834346 78-79 22.366878796977613 26.83116526362179 27.463146372723358 23.338809566677238 80-81 22.266839843960668 27.08307117047477 27.401908021284626 23.248180964279936 82-83 22.450076174982247 26.888448591998703 27.30089455045525 23.360580682563796 84-85 22.26980307034101 26.755660267126856 27.18414069100649 23.790395971525644 86-87 22.39571646373183 26.55100196439347 27.529515110308033 23.523766461566666 88-89 22.325187058583627 26.570991725677306 27.26236600445573 23.841455211283336 90-91 22.119509229927985 26.649998209259458 27.46087936923068 23.769613191581882 92-93 22.305193783817792 26.515958402991245 27.424011082349136 23.754836730841827 94-95 22.0922034819968 26.443126561685453 27.327838222869367 24.13683173344838 96-97 22.087035314314477 26.285272744431857 27.472041336353342 24.155650604900323 98-99 21.930055921219868 26.315808692071514 27.19733542086413 24.55679996584449 100-101 22.008262406108383 25.690588957654885 27.959941823852557 24.341206812384172 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 226.0 1 175.5 2 162.5 3 217.0 4 191.0 5 177.5 6 350.5 7 736.0 8 1236.0 9 1616.5 10 1626.5 11 1595.5 12 1734.5 13 1836.5 14 2004.0 15 2131.0 16 2200.0 17 2274.0 18 2341.5 19 2501.5 20 2797.5 21 3223.5 22 3818.0 23 4765.0 24 5891.5 25 7125.0 26 8846.0 27 10942.0 28 12820.5 29 15066.0 30 17749.5 31 20600.5 32 23320.0 33 25985.0 34 29811.5 35 32892.0 36 36061.5 37 39975.0 38 42813.0 39 44061.5 40 46006.0 41 50924.0 42 55568.0 43 59175.0 44 62726.0 45 66098.0 46 67031.0 47 70141.0 48 82102.5 49 84179.0 50 76708.5 51 73323.5 52 68758.5 53 66630.5 54 64963.5 55 58763.5 56 51787.5 57 45697.0 58 40160.0 59 35286.0 60 30264.5 61 24738.0 62 19956.5 63 15621.5 64 11880.5 65 9247.5 66 6934.5 67 5623.5 68 4265.0 69 2910.0 70 2184.5 71 1642.0 72 1320.0 73 1080.5 74 840.5 75 688.5 76 487.0 77 260.5 78 147.5 79 87.0 80 47.5 81 30.0 82 23.5 83 13.0 84 8.5 85 4.5 86 0.5 87 0.5 88 2.0 89 3.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3503680634201586 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.426360351615165E-4 16-17 0.001797623541677902 18-19 2.8087867838717217E-5 20-21 0.0 22-23 0.0 24-25 5.336694889356271E-4 26-27 0.0013482176562584264 28-29 0.0 30-31 0.0028649625195491563 32-33 0.0 34-35 0.010308247496809218 36-37 0.027666549821136456 38-39 0.02117825235039278 40-41 0.012948507073648637 42-43 0.009802665875712309 44-45 0.0028649625195491563 46-47 0.001179690449226123 48-49 7.583724316453648E-4 50-51 8.426360351615165E-5 52-53 5.6175735677434434E-5 54-55 0.0 56-57 0.0 58-59 0.004100828704452714 60-61 0.009718402272196157 62-63 0.00412891657229143 64-65 0.0022751172949360943 66-67 0.004353619515001169 68-69 0.003679510686871955 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.22388839454241494 78-79 6.46020960290496E-4 80-81 0.003595247083355804 82-83 8.98811770838951E-4 84-85 0.0 86-87 0.11072237502022327 88-89 0.4131444480396916 90-91 0.39988697441981697 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.001938062880871488 100-101 9.549875065163854E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1780128.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.894529021323756 #Duplication Level Percentage of deduplicated Percentage of total 1 77.20221349566822 43.151813627440596 2 13.31228024525434 14.881672690167273 3 4.19172783620668 7.028839595710348 4 1.8345852505319833 4.1017311411180986 5 0.9215686055093075 2.5755321582890423 6 0.5749430641992731 1.9281703072497047 7 0.3734612533471014 1.4612108604484653 8 0.2538628805161346 1.1351636913956746 9 0.19122135017609884 0.9619404576223203 >10 0.993693584418591 10.162985951763522 >50 0.08435502658524671 3.2683614146206637 >100 0.06035443329852866 6.121912160288924 >500 0.00462661082934455 1.781432079977571 >1k 0.0010057849629009892 1.119073055542588 >5k 1.005784962900989E-4 0.3201608083650677 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5695 0.3199208146829891 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4759 0.26734032608891045 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3085 0.1733021445648852 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2980 0.1674036923187546 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.8087867838717217E-5 2.8087867838717217E-5 16-17 0.0 0.0 0.0 8.426360351615165E-5 5.6175735677434434E-5 18-19 0.0 0.0 0.0 1.4043933919358608E-4 5.6175735677434434E-5 20-21 5.6175735677434434E-5 0.0 0.0 3.370544140646066E-4 5.6175735677434434E-5 22-23 8.426360351615165E-5 0.0 0.0 6.46020960290496E-4 5.6175735677434434E-5 24-25 1.4043933919358608E-4 0.0 0.0 9.268996386776682E-4 5.6175735677434434E-5 26-27 1.685272070323033E-4 0.0 0.0 0.0014605691276132953 5.6175735677434434E-5 28-29 1.685272070323033E-4 0.0 0.0 0.0039323014974204104 5.6175735677434434E-5 30-31 1.685272070323033E-4 0.0 0.0 0.01067338977871254 5.6175735677434434E-5 32-33 1.685272070323033E-4 0.0 0.0 0.02269499721368351 5.6175735677434434E-5 34-35 1.685272070323033E-4 0.0 0.0 0.041907098815366084 5.6175735677434434E-5 36-37 1.685272070323033E-4 0.0 0.0 0.07637091265347211 5.6175735677434434E-5 38-39 1.685272070323033E-4 0.0 0.0 0.12504718761796904 5.6175735677434434E-5 40-41 1.685272070323033E-4 0.0 0.0 0.1801274964496935 5.6175735677434434E-5 42-43 1.685272070323033E-4 0.0 0.0 0.2377357133869025 5.6175735677434434E-5 44-45 1.685272070323033E-4 0.0 0.0 0.3042758722968236 5.6175735677434434E-5 46-47 1.685272070323033E-4 0.0 0.0 0.3892697603767819 5.6175735677434434E-5 48-49 1.685272070323033E-4 0.0 0.0 0.4812575275485808 5.6175735677434434E-5 50-51 1.685272070323033E-4 0.0 0.0 0.5803234374157364 5.6175735677434434E-5 52-53 1.685272070323033E-4 0.0 0.0 0.6797264016969566 5.6175735677434434E-5 54-55 1.685272070323033E-4 0.0 0.0 0.7922744881266965 5.6175735677434434E-5 56-57 1.685272070323033E-4 0.0 0.0 0.929399458915314 5.6175735677434434E-5 58-59 1.685272070323033E-4 0.0 0.0 1.0813829117906129 5.6175735677434434E-5 60-61 1.685272070323033E-4 0.0 0.0 1.253308750831401 5.6175735677434434E-5 62-63 1.685272070323033E-4 0.0 0.0 1.4292230671052868 5.6175735677434434E-5 64-65 1.685272070323033E-4 0.0 0.0 1.609491002894174 5.6175735677434434E-5 66-67 1.685272070323033E-4 0.0 0.0 1.8187456182926172 5.6175735677434434E-5 68-69 1.685272070323033E-4 0.0 0.0 2.0632224199608116 5.6175735677434434E-5 70-71 1.685272070323033E-4 0.0 0.0 2.3292426162613022 5.6175735677434434E-5 72-73 1.685272070323033E-4 0.0 0.0 2.6123402362077335 5.6175735677434434E-5 74-75 1.685272070323033E-4 0.0 0.0 2.9108861834654585 5.6175735677434434E-5 76-77 1.685272070323033E-4 0.0 0.0 3.230273328659512 5.6175735677434434E-5 78-79 1.685272070323033E-4 0.0 0.0 3.59729749770803 5.6175735677434434E-5 80-81 2.2470294270973774E-4 0.0 0.0 3.9876626849305215 5.6175735677434434E-5 82-83 2.2470294270973774E-4 0.0 0.0 4.397296149490374 5.6175735677434434E-5 84-85 2.2470294270973774E-4 0.0 0.0 4.8224060292293585 5.6175735677434434E-5 86-87 2.2470294270973774E-4 0.0 0.0 5.2718119146488345 5.6175735677434434E-5 88-89 2.2470294270973774E-4 0.0 0.0 5.75163696093764 5.6175735677434434E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7895 0.0 22.97795 1 TACATGG 7980 0.0 22.355463 2 GAGTACT 5155 0.0 21.99908 12-13 ACATGGG 8015 0.0 21.665876 3 GTATCAA 12110 0.0 21.546406 1 GTACTTT 5695 0.0 20.079765 14-15 AGTACTT 5495 0.0 19.77439 12-13 GGTATCA 9380 0.0 19.644753 1 CATGGGG 3785 0.0 19.429628 4 ATCAACG 13750 0.0 18.874842 3 TCAACGC 13795 0.0 18.847666 4 TATCAAC 13900 0.0 18.739424 2 CAACGCA 13895 0.0 18.64373 5 CGTAAGA 155 9.701308E-4 18.366156 2 AACGCAG 14175 0.0 18.30893 6 CATGGGA 4360 0.0 17.737803 4 AGAGTAC 11170 0.0 17.140585 10-11 ACTTTTT 6770 0.0 16.9955 16-17 ACGCAGA 15365 0.0 16.860048 7 TACTTTT 6515 0.0 16.787724 14-15 >>END_MODULE